BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 17 15 35.55 2.15 2 2 20 18 31.65 3.81 3 2 21 19 24.11 0.05 4 2 24 22 24.11 0.05 5 2 49 47 29.21 2.60 6 2 50 48 24.12 0.05 7 2 51 49 35.65 1.92 8 3 17 14 36.20 1.95 9 3 51 48 35.65 1.92 10 4 9 5 801.61 5.01 11 4 13 9 45.39 2.05 12 4 14 10 36.65 2.15 13 4 17 13 36.47 2.05 14 4 51 47 36.48 2.06 15 4 52 48 36.66 2.15 16 4 53 49 57.04 0.16 17 4 129 125 60.95 1.02 18 4 132 128 59.83 0.47 19 5 9 4 802.99 4.33 20 5 53 48 57.68 0.04 21 6 9 3 807.27 2.18 22 6 53 47 59.94 0.51 23 6 54 48 60.93 1.01 24 6 55 49 61.29 0.90 25 6 129 123 60.96 1.02 26 6 132 126 60.96 1.02 27 6 133 127 60.97 1.02 28 6 136 130 61.09 0.96 29 7 55 48 61.47 0.82 30 9 13 4 817.97 1.49 31 9 129 120 78.71 0.69 32 9 175 166 85.48 0.06 33 10 13 3 841.18 4.71 34 10 14 4 835.78 7.42 35 10 175 165 89.39 0.59 36 11 14 3 847.91 1.38 37 11 15 4 841.38 4.64 38 11 172 161 79.46 0.31 39 11 173 162 87.17 0.79 40 11 175 164 92.70 0.21 41 12 15 3 847.23 1.72 42 12 16 4 841.38 4.64 43 13 16 3 854.06 1.69 44 13 17 4 841.38 4.63 45 14 17 3 841.38 4.57 46 14 18 4 830.75 0.75 47 14 129 115 98.89 1.02 48 14 157 143 93.17 0.18 49 14 161 147 93.08 0.15 50 14 168 154 93.09 0.14 51 14 171 157 95.94 0.38 52 14 172 158 94.97 0.85 53 15 18 3 846.26 2.21 54 15 19 4 831.11 0.57 55 15 168 153 93.40 0.07 56 16 19 3 851.80 0.69 57 16 20 4 831.12 0.57 58 17 20 3 847.90 8.89 59 17 21 4 831.12 0.57 60 17 50 33 105.78 0.36 61 17 52 35 115.19 0.48 62 17 158 141 99.73 1.14 63 18 21 3 841.64 5.76 64 18 22 4 831.12 0.57 65 18 157 139 104.78 0.79 66 18 158 140 99.74 1.14 67 18 161 143 98.66 0.61 68 18 162 144 98.60 0.58 69 18 168 150 99.39 0.96 70 19 22 3 845.39 2.33 71 19 23 4 826.15 1.90 72 20 23 3 835.08 2.56 73 20 24 4 826.14 1.90 74 21 24 3 840.59 5.33 75 21 25 4 835.08 2.56 76 21 50 29 118.88 0.28 77 21 64 43 118.65 0.30 78 21 158 137 118.12 0.24 79 21 162 141 112.03 0.09 80 22 25 3 848.44 1.01 81 22 161 139 118.72 0.30 82 22 162 140 114.52 0.61 83 22 164 142 118.45 0.40 84 22 165 143 118.49 0.42 85 22 168 146 118.73 0.30 86 22 238 216 109.02 1.59 87 22 239 217 105.90 0.32 88 25 37 12 118.95 0.25 89 25 64 39 119.11 0.30 90 25 66 41 118.98 0.27 91 25 69 44 119.00 0.27 92 25 239 214 119.01 0.27 93 26 37 11 119.03 0.29 94 26 38 12 119.36 0.46 95 26 41 15 119.53 0.52 96 26 66 40 119.12 0.29 97 27 30 3 936.55 0.85 98 27 35 8 119.18 0.34 99 27 36 9 119.09 0.30 100 27 38 11 119.37 0.46 101 28 38 10 119.50 0.47 102 29 35 6 122.56 1.21 103 29 36 7 121.34 1.28 104 30 33 3 834.84 9.06 105 30 34 4 512.70 91.75 106 30 35 5 122.58 1.21 107 30 36 6 122.53 1.22 108 31 34 3 815.00 5.37 109 31 36 5 410.32 142.62 110 31 199 168 159.07 0.36 111 31 200 169 151.55 3.38 112 31 203 172 164.47 1.04 113 31 240 209 125.47 0.14 114 32 199 167 164.53 1.02 115 32 202 170 164.65 1.05 116 32 203 171 164.61 1.04 117 32 207 175 164.75 1.00 118 32 228 196 164.75 1.00 119 34 233 199 164.94 1.01 120 34 237 203 164.87 1.02 121 34 238 204 164.74 1.02 122 34 239 205 164.72 1.02 123 34 240 206 164.69 1.01 124 35 238 203 164.84 1.03 125 35 239 204 164.73 1.02 126 35 240 205 164.72 1.02 127 36 200 164 164.88 1.03 128 36 240 204 164.74 1.02 129 36 241 205 164.73 1.02 130 36 242 206 163.91 1.25 131 37 66 29 164.85 1.03 132 37 67 30 164.84 1.02 133 37 68 31 164.84 1.03 134 37 69 32 164.85 1.02 135 37 192 155 164.94 1.01 136 37 239 202 164.91 1.02 137 37 240 203 164.83 1.03 138 37 241 204 164.74 1.02 139 37 242 205 164.67 0.99 140 38 41 3 919.39 4.57 141 38 67 29 164.87 1.03 142 38 242 204 164.80 1.02 143 39 242 203 164.82 1.03 144 39 244 205 164.53 0.92 145 39 245 206 163.48 1.14 146 41 45 4 810.78 7.07 147 41 46 5 735.14 6.61 148 41 66 25 710.46 11.63 149 41 67 26 692.24 20.73 150 42 45 3 845.11 0.93 151 42 46 4 810.79 7.07 152 43 46 3 826.36 0.05 153 43 47 4 836.71 5.13 154 43 94 51 640.96 7.53 155 44 94 50 653.51 1.39 156 45 66 21 717.72 13.82 157 46 65 19 717.74 13.83 158 46 66 20 717.69 13.84 159 46 67 21 717.69 13.84 160 46 90 44 686.03 0.81 161 46 94 48 684.79 0.73 162 47 64 17 717.83 13.79 163 47 65 18 717.75 13.83 164 47 66 19 717.73 13.83 165 47 72 25 717.06 13.81 166 47 90 43 686.64 0.51 167 47 91 44 685.95 0.79 168 47 94 47 685.77 0.71 169 47 95 48 683.75 0.21 170 48 62 14 718.04 13.74 171 48 63 15 717.96 13.73 172 48 64 16 717.84 13.79 173 48 66 18 717.75 13.84 174 49 62 13 718.03 13.74 175 49 63 14 717.96 13.73 176 49 95 46 686.95 0.92 177 49 96 47 686.31 0.68 178 50 61 11 718.31 13.76 179 50 62 12 718.06 13.73 180 50 64 14 718.03 13.73 181 50 158 108 462.12 3.17 182 51 59 8 718.88 13.75 183 51 60 9 718.70 13.78 184 51 61 10 718.32 13.76 185 52 59 7 718.88 13.75 186 52 60 8 718.79 13.73 187 52 62 10 718.70 13.78 188 52 132 80 536.38 1.54 189 52 136 84 538.54 1.54 190 52 154 102 506.05 1.73 191 52 158 106 467.94 0.48 192 53 57 4 727.77 9.35 193 53 58 5 719.04 13.69 194 53 59 6 718.89 13.75 195 53 136 83 539.15 1.40 196 54 57 3 812.31 0.42 197 54 58 4 727.75 9.35 198 54 133 79 538.45 1.50 199 54 136 82 539.05 1.46 200 54 137 83 539.20 1.41 201 55 130 75 539.01 1.45 202 55 133 78 539.04 1.45 203 58 136 78 539.31 1.39 204 58 140 82 539.51 1.34 205 58 141 83 539.64 1.33 206 59 140 81 539.42 1.37 207 60 74 14 717.11 13.85 208 60 75 15 716.95 13.89 209 60 76 16 716.65 13.97 210 60 136 76 539.00 1.46 211 60 139 79 539.36 1.40 212 60 140 80 539.40 1.38 213 60 154 94 506.12 1.69 214 61 74 13 716.96 13.89 215 61 75 14 716.76 13.92 216 61 96 35 687.34 0.83 217 62 71 9 717.58 13.88 218 62 73 11 717.11 13.84 219 62 74 12 716.79 13.90 220 62 96 34 687.33 0.83 221 62 154 92 505.48 1.77 222 63 71 8 717.35 13.78 223 63 72 9 717.12 13.84 224 63 73 10 717.06 13.81 225 63 95 32 687.19 0.80 226 63 96 33 687.04 0.86 227 64 70 6 717.55 13.87 228 64 71 7 717.24 13.75 229 65 68 3 859.53 1.30 230 65 69 4 725.43 9.93 231 65 70 5 717.31 13.75 232 65 72 7 717.24 13.75 233 67 241 174 169.83 0.26 234 67 245 178 164.25 0.99 235 68 163 95 444.84 1.14 236 68 192 124 283.38 1.21 237 68 241 173 167.07 1.13 238 69 159 90 457.81 1.23 239 69 162 93 444.86 1.14 240 69 163 94 444.85 1.15 241 69 192 123 283.83 1.09 242 69 239 170 193.47 1.19 243 70 85 15 688.03 0.75 244 70 109 39 533.67 0.17 245 70 110 40 532.38 0.82 246 70 163 93 444.86 1.14 247 71 83 12 688.15 0.76 248 71 154 83 505.13 1.85 249 71 155 84 494.11 1.61 250 71 158 87 462.11 3.17 251 71 159 88 458.08 1.16 252 72 82 10 688.18 0.76 253 72 83 11 688.12 0.74 254 72 87 15 687.90 0.71 255 72 88 16 687.52 0.84 256 72 91 19 687.44 0.88 257 73 81 8 688.27 0.79 258 73 82 9 688.17 0.76 259 73 96 23 687.36 0.83 260 74 80 6 688.40 0.81 261 74 81 7 688.28 0.79 262 74 96 22 687.37 0.82 263 74 139 65 539.29 1.39 264 74 142 68 539.64 1.33 265 74 151 77 535.83 0.21 266 75 78 3 909.52 0.64 267 75 79 4 814.48 10.54 268 75 80 5 688.38 0.82 269 75 96 21 687.34 0.83 270 75 98 23 687.33 0.83 271 75 99 24 685.81 0.81 272 76 79 3 858.56 3.06 273 76 81 5 796.44 0.79 274 76 140 64 539.40 1.36 275 78 99 21 686.62 0.91 276 78 100 22 686.26 0.73 277 78 101 23 685.80 0.81 278 79 101 22 679.63 0.69 279 79 103 24 677.01 2.00 280 80 96 16 687.38 0.84 281 80 99 19 686.18 0.69 282 80 101 21 679.69 0.67 283 80 102 22 657.42 0.85 284 80 103 23 654.92 2.10 285 81 101 20 679.69 0.67 286 81 103 22 614.64 6.42 287 81 105 24 572.69 2.48 288 81 142 61 539.65 1.34 289 82 88 6 692.22 1.42 290 82 91 9 687.94 0.71 291 82 92 10 687.83 0.71 292 82 95 13 687.62 0.80 293 82 96 14 687.53 0.83 294 82 102 20 657.14 0.99 295 82 103 21 606.26 10.60 296 82 104 22 572.70 2.48 297 82 105 23 559.99 8.79 298 83 88 5 757.93 3.58 299 83 105 22 572.58 2.50 300 83 107 24 542.91 0.51 301 83 108 25 535.92 0.15 302 83 109 26 535.88 0.18 303 83 114 31 538.73 1.55 304 83 151 68 535.87 0.21 305 84 104 20 572.70 2.48 306 84 105 21 572.64 2.50 307 84 107 23 542.88 0.52 308 84 108 24 538.71 1.54 309 85 88 3 842.89 0.24 310 85 89 4 825.84 1.55 311 85 108 23 535.92 0.17 312 85 109 24 535.92 0.17 313 86 89 3 840.80 0.81 314 86 90 4 833.99 4.21 315 87 90 3 843.37 0.48 316 87 91 4 834.00 4.22 317 88 91 3 840.34 1.05 318 88 92 4 850.89 4.22 319 89 92 3 852.17 3.59 320 89 93 4 849.78 4.78 321 90 93 3 855.72 1.81 322 90 94 4 850.88 4.24 323 91 94 3 853.75 2.80 324 91 95 4 842.26 1.02 325 92 102 10 639.79 5.02 326 93 97 4 846.18 4.19 327 94 97 3 888.62 1.11 328 96 99 3 856.56 3.97 329 105 142 37 541.54 0.54 330 105 151 46 539.63 1.20 331 106 113 7 770.99 6.37 332 107 113 6 770.98 6.37 333 107 114 7 698.75 3.83 334 107 117 10 626.11 39.44 335 107 147 40 542.74 0.46 336 107 148 41 542.65 0.43 337 107 151 44 542.65 0.43 338 108 113 5 772.63 5.55 339 109 114 5 811.45 2.79 340 110 113 3 855.33 3.31 341 110 114 4 823.74 2.94 342 111 114 3 836.83 5.86 343 111 115 4 823.38 3.12 344 111 122 11 545.84 1.16 345 111 152 41 542.81 0.50 346 111 155 44 542.15 0.17 347 111 156 45 541.87 0.04 348 111 160 49 505.30 1.72 349 111 189 78 300.39 1.03 350 112 115 3 846.39 1.07 351 112 116 4 827.35 1.13 352 113 116 3 843.06 2.75 353 113 117 4 824.61 2.50 354 114 117 3 834.53 3.12 355 114 118 4 821.04 1.98 356 114 148 34 543.70 0.89 357 114 151 37 543.04 0.58 358 114 152 38 543.03 0.58 359 114 155 41 542.54 0.34 360 115 118 3 827.82 0.27 361 115 119 4 819.03 0.97 362 115 120 5 783.80 11.20 363 115 121 6 626.75 39.29 364 115 122 7 626.76 39.30 365 115 152 37 543.47 0.79 366 116 119 3 799.72 10.63 367 117 148 31 543.83 0.95 368 118 148 30 543.83 0.95 369 118 149 31 543.83 0.95 370 118 152 34 543.83 0.95 371 120 124 4 821.80 2.00 372 120 125 5 821.78 2.00 373 120 126 6 834.83 4.53 374 121 124 3 851.74 3.93 375 121 125 4 851.74 3.93 376 122 125 3 851.73 3.93 377 122 152 30 543.95 0.90 378 122 156 34 543.94 0.90 379 122 181 59 427.31 0.55 380 122 186 64 381.79 3.90 381 122 189 67 351.94 8.79 382 122 190 68 347.76 6.71 383 123 180 57 429.75 0.81 384 123 181 58 427.32 0.55 385 123 183 60 426.83 0.60 386 123 186 63 387.84 0.92 387 124 180 56 429.80 0.82 388 125 149 24 544.50 0.96 389 125 152 27 544.44 0.97 390 125 153 28 544.38 0.95 391 125 180 55 429.78 0.81 392 125 181 56 429.76 0.82 393 126 131 5 779.52 11.35 394 126 153 27 544.51 0.95 395 126 178 52 430.47 0.76 396 126 179 53 430.32 0.80 397 126 180 54 429.78 0.81 398 127 131 4 809.43 4.46 399 127 153 26 544.51 0.95 400 127 156 29 544.30 1.03 401 127 171 44 442.54 0.24 402 127 178 51 430.46 0.76 403 127 179 52 430.32 0.80 404 127 181 54 427.29 0.56 405 128 131 3 851.14 5.50 406 128 132 4 837.44 2.45 407 128 171 43 442.43 0.29 408 128 174 46 436.89 0.28 409 128 178 50 430.46 0.76 410 128 179 51 430.45 0.76 411 129 132 3 845.26 8.44 412 129 133 4 835.36 3.48 413 129 171 42 442.43 0.29 414 129 174 45 436.89 0.28 415 129 175 46 433.53 0.12 416 130 133 3 835.52 3.44 417 130 134 4 822.86 2.77 418 130 174 44 439.77 1.16 419 130 175 45 433.62 0.10 420 130 176 46 433.35 0.14 421 130 177 47 430.57 0.74 422 130 178 48 430.48 0.75 423 131 134 3 844.19 6.88 424 131 135 4 822.86 2.77 425 131 153 22 544.57 0.95 426 131 178 47 430.46 0.76 427 132 135 3 832.93 0.08 428 132 136 4 822.41 2.58 429 132 153 21 544.59 0.96 430 132 154 22 544.57 0.96 431 132 157 25 544.32 1.05 432 133 136 3 839.55 2.63 433 133 137 4 825.93 0.81 434 134 137 3 838.73 3.85 435 134 138 4 824.26 3.38 436 135 138 3 829.34 0.85 437 135 139 4 827.52 1.75 438 135 150 15 544.67 0.92 439 135 153 18 544.66 0.92 440 136 139 3 847.34 0.45 441 136 140 4 820.73 1.66 442 137 140 3 841.25 3.50 443 137 141 4 820.73 1.65 444 138 141 3 840.50 0.59 445 138 142 4 820.70 1.67 446 138 143 5 817.09 0.20 447 138 146 8 544.97 0.97 448 138 147 9 544.86 0.95 449 138 150 12 544.81 0.95 450 139 142 3 817.45 0.02 451 139 147 8 544.91 0.97 452 139 150 11 544.83 0.96 453 139 151 12 544.83 0.95 454 139 154 15 544.82 0.95 455 142 147 5 771.87 16.41 456 143 146 3 861.40 0.74 457 143 147 4 811.25 2.00 458 144 147 3 838.86 9.75 459 146 149 3 869.08 5.17 460 146 150 4 831.20 5.69 461 147 150 3 831.20 5.69 462 147 151 4 831.22 5.67 463 148 151 3 842.18 0.20 464 148 152 4 833.01 4.78 465 149 152 3 833.11 4.74 466 149 153 4 822.40 0.53 467 150 153 3 846.20 1.82 468 150 154 4 820.65 0.35 469 151 154 3 822.40 0.52 470 151 155 4 820.62 0.36 471 152 155 3 831.38 1.79 472 152 156 4 827.30 0.26 473 153 156 3 832.89 2.54 474 153 157 4 823.28 2.27 475 154 157 3 837.75 4.97 476 154 158 4 831.05 4.40 477 155 158 3 852.01 2.16 478 155 159 4 808.47 5.19 479 155 160 5 793.19 2.42 480 156 160 4 793.18 2.42 481 156 161 5 793.18 2.42 482 156 167 11 443.12 0.51 483 156 171 15 442.61 0.28 484 156 189 33 295.88 0.92 485 157 161 4 808.50 5.25 486 157 171 14 442.41 0.28 487 158 161 3 835.28 3.31 488 158 162 4 819.08 6.44 489 159 162 3 824.64 3.68 490 159 163 4 822.19 4.88 491 160 163 3 829.65 1.40 492 160 164 4 825.01 3.48 493 160 167 7 664.16 58.25 494 160 189 29 290.69 1.66 495 161 164 3 842.30 2.60 496 161 167 6 443.12 0.51 497 161 168 7 442.34 0.29 498 161 171 10 442.61 0.28 499 162 238 76 194.41 1.03 500 162 239 77 193.94 1.18 501 163 192 29 284.04 1.10 502 163 237 74 194.47 1.05 503 163 238 75 194.33 0.99 504 163 239 76 193.72 1.10 505 164 167 3 839.73 0.06 506 164 168 4 827.36 3.75 507 164 188 24 300.29 1.03 508 164 191 27 298.09 0.07 509 164 192 28 287.31 0.00 510 164 195 31 284.59 1.16 511 164 236 72 194.90 0.85 512 164 237 73 194.49 1.04 513 165 168 3 835.73 0.63 514 165 169 4 834.62 0.11 515 165 236 71 194.94 0.87 516 165 238 73 194.84 0.87 517 166 169 3 854.02 9.80 518 166 170 4 821.45 1.38 519 166 184 18 350.63 5.06 520 166 185 19 345.63 5.69 521 166 188 22 316.85 2.73 522 166 236 70 194.97 0.86 523 167 170 3 846.78 4.67 524 167 171 4 821.45 1.38 525 167 185 18 332.91 0.64 526 167 188 21 300.36 1.03 527 167 189 22 300.68 1.10 528 168 171 3 849.24 2.27 529 168 172 4 831.25 0.30 530 169 172 3 839.41 0.48 531 169 173 4 835.94 1.26 532 170 173 3 860.06 0.48 533 170 174 4 843.57 3.90 534 170 179 9 430.41 0.78 535 170 185 15 350.67 5.04 536 171 174 3 865.03 2.98 537 171 179 8 603.09 85.57 538 172 175 3 878.58 2.52 539 173 177 4 873.68 0.09 540 174 177 3 850.33 3.41 541 174 178 4 797.88 4.63 542 174 179 5 764.06 5.70 543 179 185 6 834.46 1.00 544 181 185 4 834.46 1.00 545 181 186 5 812.81 2.30 546 182 185 3 847.12 5.08 547 182 186 4 834.47 0.99 548 183 186 3 834.47 0.99 549 183 187 4 824.02 4.22 550 183 216 33 243.69 4.27 551 184 187 3 835.09 0.69 552 184 188 4 820.96 6.37 553 184 216 32 243.71 4.26 554 184 217 33 243.75 4.26 555 184 218 34 243.77 4.25 556 185 188 3 840.28 2.43 557 185 189 4 820.92 6.38 558 186 189 3 833.49 2.66 559 186 190 4 818.31 7.69 560 187 190 3 830.93 1.38 561 187 191 4 818.31 7.69 562 187 215 28 243.81 4.24 563 187 216 29 243.75 4.26 564 188 191 3 835.19 1.53 565 188 192 4 819.62 6.96 566 188 215 27 243.96 4.26 567 188 232 44 196.02 0.94 568 188 236 48 195.16 0.89 569 191 194 3 843.45 2.33 570 191 195 4 821.56 6.55 571 191 213 22 257.97 3.17 572 191 215 24 261.94 5.75 573 192 195 3 835.61 0.48 574 192 196 4 821.61 6.52 575 192 237 45 195.03 0.94 576 192 239 47 195.00 0.93 577 192 240 48 193.72 1.06 578 192 241 49 193.00 0.88 579 193 196 3 833.54 0.56 580 193 197 4 823.22 5.72 581 193 241 48 192.33 1.05 582 193 246 53 174.47 2.31 583 194 197 3 829.64 3.41 584 194 198 4 824.12 6.17 585 194 213 19 263.85 5.05 586 195 198 3 849.99 6.50 587 195 199 4 832.84 1.81 588 195 209 14 264.48 4.71 589 195 213 18 263.79 5.02 590 195 215 20 264.37 4.83 591 195 225 30 263.83 5.04 592 195 232 37 219.28 12.39 593 195 237 42 195.22 0.88 594 196 199 3 845.12 3.35 595 196 200 4 836.62 0.08 596 196 240 44 194.66 0.79 597 196 241 45 193.72 1.07 598 196 246 50 175.40 2.25 599 197 200 3 844.41 3.71 600 197 201 4 836.61 0.09 601 197 246 49 177.78 2.71 602 198 201 3 844.08 3.65 603 198 202 4 836.25 0.24 604 198 209 11 264.48 4.67 605 198 210 12 264.58 4.73 606 198 211 13 264.60 4.74 607 198 213 15 264.50 4.79 608 199 202 3 836.27 0.25 609 199 203 4 836.26 0.24 610 199 209 10 264.54 4.69 611 199 233 34 243.52 1.68 612 199 237 38 196.12 0.75 613 199 240 41 195.11 0.88 614 200 203 3 847.43 4.38 615 200 204 4 837.70 0.48 616 200 241 41 195.11 0.88 617 200 242 42 194.90 0.79 618 200 243 43 194.62 0.74 619 200 246 46 193.72 0.57 620 201 204 3 837.70 0.48 621 201 205 4 835.31 0.69 622 201 210 9 264.69 4.73 623 202 205 3 834.99 0.84 624 202 206 4 820.44 8.12 625 202 207 5 337.36 17.84 626 202 208 6 264.62 4.71 627 202 209 7 264.62 4.71 628 202 228 26 264.48 4.67 629 205 208 3 832.99 0.18 630 207 227 20 265.19 4.48 631 207 228 21 264.62 4.73 632 208 226 18 265.64 4.36 633 208 227 19 265.49 4.41 634 209 212 3 880.40 0.18 635 209 213 4 858.20 1.07 636 209 225 16 265.97 4.31 637 209 226 17 265.66 4.36 638 209 227 18 265.63 4.36 639 209 228 19 265.63 4.36 640 209 233 24 257.99 0.53 641 212 224 12 266.73 4.02 642 212 225 13 266.57 4.04 643 212 226 14 266.52 4.03 644 213 224 11 267.27 3.79 645 213 225 12 266.61 4.05 646 214 222 8 268.47 3.28 647 214 223 9 267.42 3.77 648 214 224 10 267.28 3.79 649 215 222 7 268.49 3.29 650 215 223 8 267.69 3.68 651 215 225 10 267.33 3.79 652 215 232 17 258.60 0.01 653 216 220 4 317.83 2.73 654 216 221 5 268.55 3.31 655 216 222 6 268.49 3.29 656 217 220 3 827.71 6.17 657 217 221 4 521.78 97.78 658 217 223 6 268.57 3.30 659 225 230 5 309.79 0.21 660 225 232 7 309.51 0.06 661 227 230 3 854.47 1.08 662 227 233 6 312.98 0.45 663 228 233 5 312.98 0.45 664 228 234 6 312.98 0.45 665 229 234 5 595.03 73.32 666 230 233 3 839.37 2.60 667 230 234 4 814.02 5.51 668 231 234 3 830.85 2.89 669 231 235 4 744.81 19.38 670 231 236 5 687.60 27.04 671 232 236 4 472.85 80.34 672 232 237 5 472.84 80.34 673 233 237 4 565.61 8.26 674 237 240 3 936.18 0.24 675 241 245 4 808.10 2.50 676 241 246 5 794.70 9.21 677 242 245 3 837.38 2.83 678 242 246 4 808.29 2.41 679 243 246 3 837.39 2.82