BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 51 50 34.34 0.08 2 2 17 15 34.63 0.06 3 2 20 18 29.47 0.65 4 2 21 19 27.02 0.59 5 2 24 22 26.87 0.53 6 2 48 46 29.05 1.29 7 2 49 47 29.26 1.20 8 2 50 48 29.26 1.20 9 2 51 49 35.31 0.56 10 3 17 14 35.90 0.27 11 3 51 48 34.25 0.13 12 3 53 50 46.46 1.93 13 4 9 5 778.83 26.44 14 4 13 9 52.01 1.96 15 4 14 10 37.70 0.11 16 4 17 13 36.22 0.27 17 4 51 47 37.33 0.29 18 4 52 48 37.74 0.09 19 4 53 49 49.42 0.53 20 4 129 125 60.01 1.60 21 4 132 128 58.52 0.87 22 5 9 4 780.64 27.34 23 5 53 48 54.37 0.78 24 6 9 3 839.26 1.97 25 6 53 47 59.66 1.51 26 6 54 48 60.30 1.76 27 6 55 49 60.59 1.73 28 6 129 123 60.02 1.60 29 6 132 126 60.25 1.73 30 6 133 127 60.25 1.73 31 6 136 130 60.31 1.76 32 7 55 48 60.59 1.74 33 9 13 4 824.90 3.46 34 9 129 120 77.72 0.73 35 10 13 3 856.10 0.89 36 10 14 4 832.80 0.45 37 10 176 166 92.87 0.69 38 11 14 3 842.74 0.21 39 11 15 4 829.16 1.45 40 11 172 161 78.43 0.39 41 11 175 164 92.06 0.29 42 11 176 165 101.18 0.04 43 12 15 3 848.92 0.60 44 12 16 4 829.16 1.45 45 13 16 3 829.11 1.43 46 13 17 4 829.10 1.43 47 14 17 3 829.06 1.48 48 14 18 4 824.94 3.50 49 14 129 115 102.21 0.27 50 14 157 143 99.68 0.70 51 14 161 147 92.97 0.66 52 14 168 154 95.18 0.45 53 14 171 157 101.27 0.08 54 14 172 158 101.53 0.05 55 15 18 3 835.89 1.43 56 15 19 4 828.96 1.50 57 15 168 153 99.07 1.01 58 15 172 157 101.56 0.05 59 16 19 3 829.51 1.77 60 16 20 4 816.49 4.74 61 17 20 3 830.93 2.40 62 17 21 4 827.09 0.56 63 17 50 33 110.69 1.82 64 17 52 35 111.42 1.96 65 17 158 141 102.98 0.52 66 18 21 3 843.07 2.87 67 18 22 4 825.60 1.31 68 18 157 139 104.52 0.45 69 18 158 140 102.98 0.51 70 18 161 143 102.46 0.39 71 18 162 144 101.53 0.06 72 18 168 150 102.98 0.51 73 19 22 3 846.36 4.51 74 19 23 4 825.70 1.27 75 20 23 3 830.38 2.79 76 20 24 4 825.71 1.27 77 21 24 3 851.44 3.15 78 21 25 4 831.42 2.27 79 21 48 27 115.06 0.58 80 21 50 29 115.65 0.78 81 21 158 137 111.89 1.84 82 21 162 141 110.70 1.81 83 22 25 3 855.49 1.12 84 22 161 139 112.39 2.10 85 22 162 140 110.96 1.70 86 22 164 142 111.59 1.99 87 22 165 143 112.10 2.23 88 22 168 146 112.81 2.12 89 22 238 216 110.69 1.81 90 22 239 217 106.84 0.10 91 24 48 24 116.61 0.62 92 25 37 12 116.61 0.59 93 25 48 23 116.66 0.60 94 25 66 41 116.51 0.61 95 25 69 44 116.31 0.53 96 25 239 214 116.37 0.51 97 26 37 11 116.67 0.61 98 26 38 12 116.72 0.62 99 26 66 40 116.64 0.60 100 27 30 3 927.46 0.18 101 27 35 8 116.75 0.65 102 27 36 9 116.70 0.64 103 27 37 10 116.71 0.62 104 27 38 11 116.72 0.62 105 28 38 10 116.91 0.63 106 29 35 6 119.38 0.34 107 29 36 7 119.17 0.26 108 30 33 3 813.30 11.60 109 30 34 4 454.32 165.76 110 30 35 5 119.38 0.34 111 30 36 6 119.38 0.34 112 31 34 3 831.21 2.66 113 31 36 5 119.50 0.29 114 31 199 168 149.47 1.68 115 31 200 169 144.44 0.14 116 31 203 172 152.36 0.70 117 31 240 209 120.16 0.62 118 32 199 167 152.30 0.68 119 32 202 170 152.39 0.70 120 32 203 171 152.38 0.70 121 32 207 175 152.42 0.71 122 32 228 196 152.43 0.73 123 34 199 165 152.60 0.65 124 34 233 199 152.75 0.64 125 34 237 203 152.68 0.64 126 34 238 204 152.66 0.63 127 34 239 205 152.60 0.64 128 34 240 206 152.47 0.67 129 35 238 203 152.80 0.59 130 35 239 204 152.62 0.64 131 35 240 205 152.53 0.68 132 36 200 164 152.72 0.61 133 36 240 204 152.69 0.61 134 36 242 206 152.33 0.69 135 37 67 30 152.75 0.58 136 37 68 31 152.75 0.58 137 37 69 32 152.78 0.57 138 37 192 155 152.90 0.62 139 37 239 202 152.85 0.60 140 37 240 203 152.77 0.59 141 37 242 205 152.47 0.67 142 38 41 3 908.04 3.12 143 38 67 29 361.73 103.84 144 38 242 204 152.72 0.60 145 39 242 203 152.73 0.60 146 39 244 205 152.46 0.67 147 39 245 206 151.12 0.69 148 40 46 6 695.56 60.61 149 40 67 27 362.11 103.95 150 41 45 4 824.00 2.52 151 41 46 5 677.66 53.83 152 41 66 25 470.85 71.61 153 41 67 26 389.47 117.58 154 43 46 3 859.30 3.52 155 43 47 4 861.55 1.74 156 43 94 51 533.38 18.26 157 44 47 3 852.43 0.10 158 44 94 50 533.39 18.27 159 45 66 21 642.23 48.36 160 46 65 19 642.29 48.35 161 46 66 20 642.23 48.36 162 46 67 21 642.14 48.38 163 47 64 17 642.33 48.34 164 47 65 18 642.30 48.35 165 47 66 19 642.29 48.34 166 47 72 25 641.15 48.12 167 47 90 43 605.31 33.05 168 47 91 44 605.19 33.07 169 47 94 47 607.10 31.96 170 47 95 48 604.57 32.48 171 48 62 14 642.79 48.47 172 48 63 15 642.41 48.38 173 48 64 16 642.34 48.35 174 48 66 18 642.30 48.34 175 49 61 12 642.79 48.47 176 49 62 13 642.78 48.47 177 49 63 14 642.52 48.39 178 49 95 46 607.43 32.00 179 49 96 47 607.40 31.99 180 50 60 10 643.24 48.41 181 50 61 11 642.82 48.47 182 50 62 12 642.79 48.47 183 50 64 14 642.75 48.48 184 50 71 21 642.06 48.30 185 50 158 108 444.18 2.26 186 51 60 9 643.25 48.41 187 51 61 10 643.03 48.36 188 52 59 7 643.29 48.40 189 52 60 8 643.25 48.41 190 52 62 10 643.21 48.39 191 52 132 80 544.86 1.34 192 52 136 84 545.52 1.19 193 52 154 102 511.94 0.80 194 52 158 106 469.94 2.98 195 53 57 4 691.37 24.59 196 53 58 5 643.46 48.41 197 53 59 6 643.29 48.40 198 53 136 83 545.62 1.15 199 54 57 3 807.70 0.28 200 54 58 4 643.57 48.45 201 54 133 79 545.57 1.18 202 54 136 82 545.62 1.15 203 54 137 83 545.65 1.16 204 55 129 74 545.91 1.29 205 55 130 75 545.91 1.29 206 55 133 78 545.91 1.29 207 58 136 78 545.86 1.27 208 58 140 82 545.64 1.14 209 59 140 81 545.61 1.14 210 60 75 15 641.14 48.11 211 60 136 76 545.49 1.19 212 60 139 79 545.58 1.16 213 60 140 80 545.56 1.12 214 61 74 13 641.19 48.13 215 61 75 14 640.87 48.08 216 61 96 35 607.78 31.94 217 62 71 9 642.02 48.33 218 62 73 11 641.20 48.13 219 62 74 12 640.80 48.12 220 62 154 92 506.33 0.15 221 63 71 8 641.98 48.34 222 63 72 9 641.26 48.16 223 63 73 10 641.18 48.12 224 63 95 32 607.41 32.01 225 63 96 33 607.43 32.00 226 64 69 5 642.07 48.34 227 64 70 6 642.00 48.35 228 64 71 7 641.31 48.19 229 65 68 3 861.96 1.28 230 65 69 4 642.66 48.04 231 65 70 5 641.58 48.15 232 65 72 7 641.18 48.13 233 67 241 174 156.16 1.15 234 68 163 95 426.09 3.06 235 68 192 124 290.67 0.69 236 68 241 173 156.77 0.84 237 69 159 90 443.56 2.33 238 69 163 94 425.64 3.25 239 69 192 123 293.21 1.95 240 69 239 170 177.00 4.58 241 70 85 15 605.98 33.19 242 70 109 39 531.22 0.00 243 70 110 40 528.60 1.17 244 71 83 12 605.98 33.23 245 71 154 83 502.59 1.86 246 71 155 84 489.45 1.58 247 71 158 87 453.02 0.13 248 72 82 10 605.98 33.23 249 72 83 11 605.97 33.22 250 72 87 15 605.90 33.12 251 72 88 16 605.76 33.07 252 72 91 19 605.82 33.02 253 73 81 8 606.12 33.27 254 73 82 9 606.09 33.28 255 73 96 23 710.94 19.55 256 74 80 6 628.34 22.21 257 74 81 7 606.19 33.26 258 74 96 22 720.01 24.08 259 74 139 65 545.59 1.15 260 74 151 77 537.37 0.93 261 75 78 3 895.46 3.27 262 75 79 4 761.23 8.12 263 75 80 5 628.35 22.21 264 75 96 21 720.00 24.09 265 75 99 24 727.45 28.08 266 76 79 3 852.88 3.06 267 76 81 5 802.64 4.33 268 76 139 63 545.62 1.15 269 76 140 64 545.58 1.12 270 78 99 21 727.94 28.33 271 78 100 22 727.92 28.32 272 78 101 23 727.89 28.34 273 79 99 20 727.96 28.34 274 79 101 22 727.64 28.45 275 79 103 24 718.49 32.95 276 80 96 16 728.27 28.21 277 80 99 19 728.02 28.34 278 80 101 21 727.71 28.48 279 80 102 22 673.32 55.63 280 80 103 23 672.31 56.13 281 81 103 22 672.25 56.17 282 81 105 24 672.04 56.18 283 81 142 61 569.43 11.92 284 82 88 6 728.57 28.20 285 82 92 10 728.42 28.21 286 82 95 13 728.43 28.21 287 82 96 14 728.41 28.21 288 82 102 20 672.38 56.17 289 82 103 21 672.32 56.18 290 82 104 22 672.13 56.22 291 82 105 23 672.12 56.22 292 83 88 5 781.30 1.85 293 83 104 21 672.19 56.25 294 83 105 22 672.13 56.23 295 83 107 24 669.09 57.60 296 83 108 25 661.33 61.29 297 83 109 26 653.28 58.80 298 83 114 31 617.65 39.45 299 83 151 68 569.54 14.66 300 84 104 20 672.20 56.25 301 84 105 21 672.18 56.25 302 84 107 23 668.17 58.10 303 84 108 24 661.45 61.34 304 85 88 3 840.21 3.33 305 85 89 4 826.46 1.04 306 85 108 23 661.51 61.38 307 85 109 24 660.00 62.14 308 86 89 3 845.65 8.12 309 86 90 4 831.39 1.42 310 87 90 3 845.32 8.29 311 87 91 4 831.46 1.44 312 88 91 3 847.06 7.42 313 88 92 4 833.28 0.54 314 89 92 3 867.42 0.28 315 89 93 4 850.10 8.94 316 89 102 13 718.51 33.13 317 90 93 3 864.39 1.24 318 90 94 4 854.29 3.81 319 91 94 3 868.28 0.15 320 91 95 4 833.28 0.54 321 92 97 5 816.49 8.81 322 92 102 10 672.38 56.20 323 93 97 4 829.82 2.14 324 94 97 3 897.53 0.80 325 96 99 3 846.19 4.28 326 103 142 39 590.71 21.06 327 105 142 37 591.70 21.25 328 105 151 46 590.32 21.25 329 106 113 7 796.61 2.98 330 106 142 36 592.16 21.41 331 106 144 38 591.90 21.50 332 107 113 6 794.07 4.25 333 107 114 7 734.26 25.64 334 107 117 10 623.07 36.27 335 107 144 37 592.18 21.43 336 107 147 40 592.15 21.41 337 107 148 41 592.04 21.43 338 107 151 44 591.95 21.47 339 108 113 5 798.64 1.97 340 108 114 6 799.07 4.19 341 109 114 5 812.81 0.42 342 110 113 3 850.34 1.50 343 110 114 4 816.50 2.25 344 111 114 3 843.65 5.32 345 111 115 4 818.53 1.23 346 111 122 11 592.90 21.25 347 111 152 41 592.13 21.38 348 111 155 44 591.34 21.78 349 111 156 45 591.23 21.82 350 111 160 49 546.13 20.30 351 112 115 3 854.58 0.92 352 112 116 4 826.58 5.25 353 113 116 3 844.23 5.60 354 113 117 4 818.53 1.22 355 114 117 3 838.55 8.44 356 114 118 4 814.88 0.62 357 114 148 34 592.22 21.39 358 114 151 37 592.21 21.39 359 114 152 38 592.15 21.38 360 114 155 41 591.38 21.79 361 115 118 3 818.51 0.18 362 115 119 4 813.38 1.36 363 115 120 5 793.88 4.41 364 115 121 6 595.31 20.84 365 115 122 7 595.30 20.84 366 115 152 37 592.84 21.71 367 116 119 3 821.51 1.24 368 117 145 28 593.28 21.80 369 117 148 31 593.25 21.80 370 118 145 27 593.28 21.80 371 118 148 30 593.25 21.80 372 118 149 31 593.25 21.80 373 118 152 34 593.25 21.80 374 120 124 4 814.56 0.91 375 120 125 5 814.24 1.06 376 121 124 3 854.75 0.21 377 121 125 4 834.28 3.94 378 122 125 3 855.64 0.25 379 122 152 30 593.04 21.98 380 122 156 34 590.38 23.21 381 122 181 59 411.25 1.73 382 122 186 64 359.86 3.90 383 122 189 67 352.63 7.66 384 122 190 68 340.06 1.68 385 123 180 57 411.43 1.77 386 123 181 58 411.39 1.78 387 123 186 63 365.11 3.66 388 124 180 56 411.44 1.77 389 125 152 27 591.88 23.19 390 125 153 28 591.41 23.39 391 125 180 55 411.44 1.76 392 125 181 56 411.44 1.77 393 126 131 5 800.88 5.92 394 126 153 27 591.11 23.62 395 126 178 52 412.30 1.59 396 126 179 53 412.24 1.60 397 126 180 54 411.44 1.77 398 127 131 4 800.94 5.88 399 127 153 26 589.47 24.44 400 127 156 29 588.72 24.79 401 127 157 30 588.33 24.82 402 127 171 44 425.31 3.16 403 127 174 47 424.47 2.79 404 127 178 51 412.29 1.59 405 127 179 52 412.12 1.54 406 127 181 54 410.90 1.81 407 128 131 3 827.88 4.32 408 128 132 4 825.32 3.88 409 128 157 29 585.93 26.16 410 128 171 43 425.25 3.17 411 128 174 46 424.19 2.91 412 128 178 50 412.29 1.59 413 129 132 3 837.62 2.27 414 129 133 4 825.46 3.81 415 129 171 42 425.23 3.16 416 129 174 45 424.20 2.92 417 129 175 46 421.89 1.83 418 130 133 3 841.74 3.46 419 130 134 4 826.21 3.45 420 130 174 44 424.45 3.00 421 130 175 45 422.15 1.93 422 130 176 46 421.26 1.58 423 130 177 47 419.37 1.84 424 130 178 48 415.23 0.14 425 131 134 3 827.06 3.02 426 131 135 4 826.21 3.46 427 131 153 22 589.54 24.59 428 131 178 47 412.30 1.59 429 132 135 3 830.63 3.15 430 132 136 4 828.71 2.21 431 132 153 21 589.50 24.61 432 132 154 22 588.73 25.00 433 132 157 25 574.50 32.02 434 133 136 3 844.33 3.71 435 133 137 4 830.61 3.15 436 134 137 3 844.33 3.71 437 134 138 4 830.61 3.15 438 135 138 3 840.31 0.18 439 135 139 4 828.78 2.27 440 135 150 15 589.69 24.54 441 135 153 18 589.51 24.62 442 136 139 3 842.98 0.60 443 136 140 4 842.97 0.60 444 137 140 3 845.89 0.85 445 137 141 4 854.55 5.18 446 138 141 3 861.93 3.36 447 138 146 8 594.57 22.22 448 138 147 9 594.57 22.23 449 138 150 12 594.50 22.21 450 139 147 8 594.57 22.22 451 139 150 11 594.51 22.21 452 139 151 12 594.52 22.22 453 139 154 15 594.37 22.29 454 143 146 3 832.40 0.21 455 143 147 4 827.57 1.52 456 144 147 3 838.86 0.88 457 144 148 4 836.28 2.17 458 145 148 3 846.12 2.75 459 145 149 4 836.30 2.17 460 146 149 3 850.37 0.62 461 146 150 4 832.63 4.00 462 147 150 3 845.16 2.26 463 147 151 4 832.63 4.00 464 148 151 3 850.28 2.83 465 148 152 4 839.39 0.62 466 149 152 3 849.69 2.54 467 149 153 4 836.47 1.82 468 150 153 3 843.81 5.48 469 150 154 4 823.02 4.90 470 151 154 3 836.46 1.81 471 151 155 4 823.03 4.90 472 152 155 3 856.40 2.31 473 152 156 4 831.28 9.02 474 153 156 3 846.25 1.54 475 153 157 4 820.40 3.60 476 154 157 3 851.73 1.21 477 154 158 4 818.50 4.55 478 155 158 3 851.75 1.20 479 155 159 4 808.22 1.07 480 155 160 5 808.22 1.07 481 156 160 4 802.53 1.77 482 156 161 5 802.53 1.77 483 156 167 11 425.81 3.32 484 156 181 25 399.05 0.38 485 156 186 30 342.61 0.82 486 156 189 33 331.29 2.44 487 157 161 4 808.41 0.60 488 157 171 14 425.13 3.15 489 158 161 3 842.73 2.17 490 158 162 4 808.52 0.56 491 159 162 3 826.99 1.61 492 159 163 4 815.07 3.81 493 160 163 3 832.21 0.99 494 160 164 4 808.51 0.55 495 160 167 7 425.84 3.31 496 160 189 29 330.45 2.81 497 161 164 3 837.35 3.59 498 161 167 6 425.80 3.33 499 161 168 7 425.69 3.35 500 162 238 76 209.59 12.39 501 162 239 77 209.53 12.39 502 163 192 29 309.87 0.64 503 163 237 74 210.37 12.71 504 163 238 75 209.83 12.51 505 163 239 76 209.54 12.38 506 164 167 3 854.26 2.54 507 164 168 4 829.86 0.93 508 164 188 24 334.76 2.92 509 164 189 25 334.78 2.93 510 164 192 28 319.00 2.69 511 164 195 31 311.26 1.24 512 164 236 72 210.46 12.69 513 164 237 73 210.28 12.67 514 164 238 74 209.78 12.43 515 165 168 3 841.43 3.88 516 165 169 4 829.90 0.94 517 165 235 70 210.50 12.71 518 165 236 71 210.50 12.71 519 165 238 73 210.21 12.61 520 166 169 3 841.44 3.88 521 166 170 4 832.72 0.48 522 166 182 16 407.75 0.60 523 166 184 18 364.47 3.98 524 166 185 19 363.58 4.48 525 166 188 22 357.74 4.32 526 166 236 70 210.56 12.67 527 167 170 3 834.91 0.62 528 167 171 4 834.92 0.63 529 167 185 18 363.50 4.45 530 167 188 21 343.69 0.21 531 167 189 22 342.35 0.85 532 168 171 3 844.25 3.64 533 168 172 4 832.12 2.04 534 169 172 3 840.10 1.95 535 169 173 4 822.27 6.96 536 170 173 3 832.18 2.10 537 170 174 4 821.62 7.29 538 170 182 12 407.97 0.70 539 170 185 15 367.98 2.27 540 171 174 3 837.61 3.18 541 171 175 4 819.04 6.02 542 172 175 3 832.80 1.64 543 174 177 3 832.79 1.64 544 174 178 4 589.75 18.38 545 174 179 5 606.27 98.53 546 174 180 6 714.93 44.21 547 181 185 4 809.26 5.45 548 181 186 5 791.19 9.10 549 182 185 3 838.78 3.17 550 182 186 4 837.20 3.96 551 183 186 3 838.75 3.19 552 183 187 4 804.01 2.71 553 183 216 33 277.22 1.09 554 183 221 38 277.48 0.97 555 184 187 3 845.55 0.21 556 184 188 4 820.32 6.02 557 184 217 33 277.28 1.14 558 184 218 34 277.79 1.06 559 185 188 3 846.72 0.37 560 185 189 4 819.60 5.66 561 186 189 3 839.19 4.13 562 186 190 4 838.06 3.45 563 187 190 3 840.65 2.15 564 187 191 4 838.06 3.45 565 187 215 28 280.33 0.32 566 187 216 29 280.53 0.42 567 187 217 30 280.56 0.40 568 188 191 3 838.06 3.45 569 188 192 4 819.32 5.80 570 188 215 27 280.56 0.42 571 188 217 29 280.58 0.42 572 188 232 44 212.97 12.10 573 188 236 48 212.62 11.92 574 191 194 3 836.36 2.25 575 191 195 4 825.20 7.51 576 191 213 22 280.72 0.36 577 191 215 24 280.70 0.37 578 192 195 3 836.15 2.04 579 192 196 4 835.78 2.22 580 192 237 45 212.83 11.93 581 192 239 47 212.63 11.90 582 192 240 48 210.58 11.89 583 192 241 49 207.91 10.80 584 193 196 3 835.81 2.22 585 193 197 4 835.81 2.21 586 193 241 48 208.38 12.34 587 193 246 53 112.67 26.34 588 194 197 3 844.61 0.27 589 194 198 4 836.03 2.09 590 194 213 19 281.65 0.24 591 195 198 3 836.25 2.44 592 195 199 4 835.79 2.21 593 195 209 14 282.15 0.40 594 195 213 18 281.65 0.23 595 195 232 37 225.44 6.46 596 195 233 38 214.09 12.11 597 195 237 42 213.37 11.88 598 196 200 4 808.46 11.43 599 196 240 44 210.61 11.88 600 196 241 45 209.93 11.58 601 196 246 50 178.48 1.89 602 197 200 3 849.59 4.58 603 197 201 4 810.24 12.37 604 197 243 46 206.19 11.54 605 197 246 49 178.50 1.90 606 198 201 3 830.28 2.35 607 198 202 4 815.18 9.89 608 198 209 11 282.28 0.33 609 198 210 12 282.47 0.38 610 198 211 13 282.48 0.37 611 198 213 15 282.41 0.34 612 199 202 3 837.00 1.01 613 199 209 10 282.49 0.39 614 199 233 34 226.50 6.98 615 199 237 38 214.00 11.90 616 199 240 41 213.44 11.86 617 200 203 3 847.25 0.32 618 200 240 40 213.47 11.86 619 200 241 41 213.46 11.85 620 200 242 42 213.27 11.83 621 200 243 43 210.98 11.96 622 201 205 4 819.61 5.62 623 201 210 9 282.66 0.34 624 202 205 3 813.08 8.08 625 202 206 4 787.80 9.18 626 202 207 5 471.03 89.83 627 202 208 6 290.68 2.78 628 202 228 26 282.44 0.38 629 205 208 3 821.25 4.71 630 205 210 5 742.62 30.37 631 207 227 20 282.93 0.31 632 207 228 21 282.74 0.27 633 208 226 18 283.32 0.48 634 208 227 19 283.29 0.48 635 209 212 3 855.81 8.90 636 209 213 4 837.06 0.47 637 209 225 16 283.46 0.53 638 209 226 17 283.33 0.48 639 209 227 18 283.31 0.48 640 209 228 19 283.31 0.48 641 209 233 24 262.64 10.83 642 212 224 12 284.11 0.56 643 212 225 13 283.92 0.58 644 212 226 14 283.68 0.48 645 213 224 11 284.16 0.58 646 213 225 12 283.93 0.59 647 214 222 8 286.86 0.43 648 214 223 9 285.55 0.96 649 214 224 10 284.18 0.59 650 215 222 7 286.93 0.44 651 215 223 8 285.95 0.79 652 215 225 10 285.54 0.96 653 215 232 17 283.91 0.61 654 216 219 3 881.12 1.65 655 216 220 4 786.24 10.66 656 216 221 5 286.95 0.43 657 216 222 6 286.93 0.44 658 216 223 7 286.88 0.45 659 217 220 3 877.00 5.68 660 217 223 6 297.38 2.05 661 218 223 5 686.35 1.62 662 219 222 3 869.49 0.14 663 219 223 4 744.74 30.82 664 225 230 5 295.20 3.05 665 225 232 7 295.72 2.69 666 225 233 8 298.44 2.12 667 227 230 3 871.93 1.40 668 228 233 5 302.08 1.33 669 228 234 6 304.28 0.24 670 229 234 5 305.14 0.67 671 230 233 3 823.92 7.95 672 230 234 4 746.44 29.82 673 231 234 3 780.67 12.71 674 231 235 4 560.57 2.94 675 231 236 5 456.68 49.01 676 232 236 4 305.14 0.67 677 232 237 5 305.13 0.67 678 233 237 4 514.99 105.59 679 237 240 3 931.22 0.98 680 241 245 4 825.11 0.90 681 241 246 5 768.85 28.80 682 242 245 3 844.85 0.74 683 242 246 4 833.44 5.07 684 243 246 3 823.17 3.52