BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 51 50 39.53 0.75 2 2 17 15 39.52 0.76 3 2 20 18 37.19 0.12 4 2 21 19 34.99 0.55 5 2 24 22 35.00 0.54 6 2 48 46 35.02 0.54 7 2 49 47 35.26 0.54 8 2 50 48 35.25 0.54 9 2 51 49 39.55 0.76 10 3 17 14 39.96 0.77 11 3 51 48 39.53 0.75 12 3 53 50 46.55 1.98 13 4 9 5 749.26 12.46 14 4 13 9 51.14 0.94 15 4 14 10 43.24 1.54 16 4 17 13 42.07 1.26 17 4 51 47 42.23 1.18 18 4 52 48 42.28 1.17 19 4 53 49 47.14 1.69 20 4 129 125 63.00 0.35 21 4 132 128 62.72 0.23 22 5 9 4 809.09 11.30 23 5 53 48 54.06 2.31 24 6 9 3 841.72 0.74 25 6 53 47 62.93 0.37 26 6 54 48 63.01 0.35 27 6 55 49 63.22 0.44 28 6 129 123 63.01 0.35 29 6 132 126 63.02 0.35 30 6 133 127 63.18 0.43 31 6 136 130 63.19 0.43 32 7 55 48 65.53 0.70 33 9 13 4 822.27 2.17 34 9 129 120 77.70 0.74 35 10 13 3 848.59 4.65 36 10 14 4 829.02 1.39 37 10 176 166 92.73 0.75 38 11 14 3 845.02 1.35 39 11 15 4 829.04 1.39 40 11 172 161 77.67 0.70 41 11 175 164 91.66 0.38 42 11 176 165 101.24 0.06 43 12 15 3 848.92 0.60 44 12 16 4 835.75 1.78 45 13 16 3 829.22 1.49 46 13 17 4 829.09 1.42 47 14 17 3 835.70 1.28 48 14 18 4 822.75 4.67 49 14 129 115 102.08 0.34 50 14 157 143 99.67 0.70 51 14 161 147 96.80 0.12 52 14 168 154 97.73 0.58 53 14 171 157 101.28 0.07 54 14 172 158 101.37 0.03 55 15 18 3 830.81 2.35 56 15 19 4 827.56 2.28 57 15 168 153 99.99 0.55 58 15 172 157 101.45 0.01 59 16 19 3 831.53 1.98 60 16 20 4 827.58 2.27 61 17 20 3 830.85 2.44 62 17 21 4 827.58 2.27 63 17 50 33 107.52 0.23 64 17 52 35 111.27 1.82 65 17 158 141 102.44 0.26 66 18 21 3 842.26 3.27 67 18 22 4 827.71 2.21 68 18 157 139 104.53 0.45 69 18 158 140 103.19 0.62 70 18 161 143 102.11 0.32 71 18 162 144 101.43 0.00 72 18 168 150 102.41 0.23 73 19 22 3 845.43 4.98 74 19 23 4 827.65 2.18 75 20 23 3 842.75 3.02 76 20 24 4 829.43 1.28 77 21 24 3 853.26 2.23 78 21 25 4 831.42 2.27 79 21 48 27 118.02 0.42 80 21 50 29 118.25 0.36 81 21 69 48 107.65 0.29 82 21 158 137 112.32 2.35 83 21 162 141 110.43 1.68 84 22 25 3 855.49 1.12 85 22 161 139 112.71 2.25 86 22 162 140 111.58 1.99 87 22 164 142 111.60 1.99 88 22 165 143 112.38 2.35 89 22 168 146 117.00 0.13 90 22 238 216 107.54 0.24 91 22 239 217 107.52 0.25 92 24 48 24 119.00 0.52 93 25 37 12 118.89 0.53 94 25 48 23 119.00 0.52 95 25 66 41 118.78 0.47 96 25 69 44 118.83 0.50 97 25 239 214 118.75 0.49 98 26 37 11 118.92 0.53 99 26 38 12 118.98 0.53 100 26 66 40 119.00 0.52 101 27 30 3 949.62 3.14 102 27 35 8 119.02 0.52 103 27 36 9 118.95 0.53 104 27 37 10 118.94 0.56 105 27 38 11 118.98 0.53 106 28 38 10 119.10 0.57 107 29 35 6 339.17 110.20 108 29 36 7 339.00 110.26 109 30 33 3 828.43 1.26 110 30 34 4 747.41 19.22 111 30 35 5 339.18 110.20 112 30 36 6 339.13 110.21 113 31 34 3 848.17 8.71 114 31 36 5 339.14 110.21 115 31 199 168 150.84 2.36 116 31 200 169 140.66 2.03 117 31 203 172 156.24 0.57 118 31 240 209 123.59 0.65 119 32 199 167 156.72 0.48 120 32 202 170 157.09 0.66 121 32 203 171 157.09 0.65 122 32 207 175 157.11 0.65 123 32 228 196 157.30 0.56 124 34 199 165 157.81 0.45 125 34 233 199 157.97 0.41 126 34 237 203 157.91 0.43 127 34 238 204 157.89 0.42 128 34 239 205 157.77 0.46 129 34 240 206 157.72 0.45 130 35 238 203 157.96 0.42 131 35 239 204 157.84 0.44 132 35 240 205 157.77 0.46 133 36 200 164 157.89 0.42 134 36 240 204 157.84 0.44 135 36 242 206 156.95 0.70 136 37 66 29 157.99 0.45 137 37 67 30 157.96 0.43 138 37 68 31 157.93 0.42 139 37 69 32 157.97 0.43 140 37 192 155 158.03 0.43 141 37 239 202 158.02 0.42 142 37 240 203 157.91 0.42 143 37 241 204 157.87 0.42 144 37 242 205 157.72 0.46 145 38 41 3 898.72 1.54 146 38 67 29 158.00 0.45 147 38 242 204 157.87 0.42 148 39 242 203 157.88 0.42 149 39 244 205 157.47 0.52 150 39 245 206 157.08 0.67 151 40 46 6 758.35 29.22 152 40 67 27 158.17 0.46 153 41 45 4 826.70 3.56 154 41 46 5 736.26 40.26 155 41 66 25 494.70 27.56 156 41 67 26 353.58 98.12 157 43 46 3 863.61 1.37 158 43 47 4 861.55 1.74 159 43 94 51 496.76 0.04 160 44 47 3 863.61 1.37 161 44 94 50 496.78 0.04 162 45 66 21 548.83 3.49 163 46 65 19 548.82 3.49 164 46 66 20 548.82 3.49 165 46 67 21 548.82 3.49 166 47 64 17 548.86 3.48 167 47 65 18 548.83 3.49 168 47 66 19 548.83 3.49 169 47 72 25 547.00 3.73 170 47 90 43 538.67 0.23 171 47 91 44 538.63 0.21 172 47 94 47 538.62 0.21 173 47 95 48 538.48 0.14 174 48 62 14 549.36 3.44 175 48 63 15 549.08 3.48 176 48 64 16 548.90 3.50 177 48 66 18 548.84 3.49 178 49 61 12 549.53 3.38 179 49 62 13 549.36 3.44 180 49 63 14 549.25 3.43 181 49 95 46 539.00 0.19 182 49 96 47 538.89 0.11 183 50 61 11 549.59 3.39 184 50 62 12 549.38 3.44 185 50 64 14 549.33 3.46 186 50 71 21 548.71 3.50 187 50 158 108 455.35 1.07 188 51 60 9 549.77 3.31 189 51 61 10 549.63 3.37 190 52 59 7 549.78 3.33 191 52 60 8 549.77 3.32 192 52 62 10 549.72 3.33 193 52 136 84 535.19 1.24 194 52 154 102 515.59 2.39 195 52 158 106 475.20 1.18 196 53 57 4 651.92 54.31 197 53 58 5 550.82 3.77 198 53 59 6 549.83 3.32 199 53 136 83 535.41 1.15 200 54 57 3 809.96 1.41 201 54 58 4 572.97 14.84 202 54 133 79 535.15 1.25 203 54 136 82 535.42 1.15 204 54 137 83 535.47 1.14 205 55 129 74 535.41 1.15 206 55 130 75 535.41 1.15 207 55 133 78 535.42 1.15 208 58 140 82 535.70 1.14 209 59 140 81 535.63 1.16 210 60 75 15 546.91 3.74 211 60 76 16 546.79 3.79 212 60 136 76 535.20 1.26 213 60 139 79 535.52 1.17 214 60 140 80 535.56 1.15 215 60 154 94 515.88 2.29 216 61 74 13 547.03 3.69 217 61 75 14 546.79 3.79 218 61 96 35 539.22 0.07 219 62 71 9 548.71 3.48 220 62 73 11 547.09 3.71 221 62 74 12 546.81 3.79 222 62 154 92 507.05 1.62 223 63 71 8 548.60 3.52 224 63 72 9 547.38 3.86 225 63 73 10 547.06 3.71 226 63 95 32 539.00 0.18 227 63 96 33 538.95 0.12 228 64 69 5 548.79 3.51 229 64 70 6 548.68 3.48 230 64 71 7 547.20 3.76 231 65 68 3 858.25 0.57 232 65 69 4 548.77 3.52 233 65 70 5 547.69 3.85 234 65 72 7 547.07 3.71 235 67 241 174 173.88 5.15 236 68 163 95 433.83 0.01 237 68 192 124 301.35 4.61 238 68 241 173 173.88 5.15 239 69 159 90 447.58 2.67 240 69 162 93 433.84 0.01 241 69 163 94 433.81 0.01 242 69 192 123 303.92 3.36 243 69 239 170 189.20 0.06 244 70 85 15 539.10 0.30 245 70 109 39 534.12 1.64 246 70 110 40 534.12 1.64 247 71 83 12 539.10 0.30 248 71 154 83 501.28 1.20 249 71 155 84 483.55 1.29 250 72 82 10 539.09 0.29 251 72 83 11 539.09 0.30 252 72 87 15 539.11 0.29 253 72 88 16 538.99 0.24 254 72 91 19 539.02 0.21 255 73 81 8 539.13 0.29 256 73 82 9 539.09 0.29 257 74 80 6 539.25 0.26 258 74 81 7 539.16 0.27 259 74 96 22 663.01 61.80 260 74 105 31 536.61 1.03 261 74 139 65 535.56 1.16 262 74 147 73 535.56 1.15 263 74 151 77 535.12 1.07 264 75 78 3 895.46 3.27 265 75 79 4 781.46 8.23 266 75 80 5 539.26 0.26 267 75 96 21 663.02 61.80 268 75 99 24 667.56 64.22 269 76 79 3 852.88 3.06 270 76 81 5 807.15 2.06 271 76 139 63 535.57 1.16 272 76 140 64 535.56 1.16 273 78 99 21 667.81 64.29 274 78 100 22 667.74 64.32 275 78 101 23 667.72 64.31 276 79 99 20 667.78 64.32 277 79 101 22 666.51 64.92 278 79 103 24 665.60 65.36 279 80 96 16 667.94 64.25 280 80 99 19 667.80 64.34 281 80 101 21 667.09 64.66 282 80 102 22 665.57 65.40 283 80 103 23 665.51 65.43 284 81 103 22 665.47 65.45 285 81 105 24 665.22 65.55 286 81 142 61 572.27 19.25 287 82 88 6 668.72 64.00 288 82 91 9 668.33 64.19 289 82 92 10 668.10 64.28 290 82 95 13 668.09 64.28 291 82 96 14 668.05 64.28 292 82 102 20 665.67 65.41 293 82 103 21 665.48 65.47 294 82 104 22 665.25 65.56 295 82 105 23 665.21 65.56 296 83 88 5 757.81 19.47 297 83 104 21 665.45 65.47 298 83 105 22 665.18 65.58 299 83 107 24 664.95 65.59 300 83 108 25 656.09 61.19 301 83 109 26 638.88 52.59 302 83 114 31 616.11 41.20 303 83 151 68 569.66 18.27 304 84 104 20 665.28 65.58 305 84 105 21 665.24 65.58 306 84 107 23 664.97 65.63 307 84 108 24 664.50 65.41 308 85 88 3 855.06 2.78 309 85 89 4 831.81 1.20 310 85 108 23 664.91 65.61 311 85 109 24 664.91 65.61 312 86 89 3 845.66 8.12 313 86 90 4 826.46 1.04 314 87 90 3 845.23 8.34 315 87 91 4 845.33 8.29 316 88 91 3 845.33 8.29 317 88 92 4 847.06 7.42 318 89 92 3 867.43 0.28 319 89 93 4 850.10 8.94 320 89 102 13 665.71 65.42 321 90 93 3 867.42 0.28 322 90 94 4 850.31 0.07 323 91 94 3 868.28 0.16 324 91 95 4 833.15 0.48 325 92 97 5 820.28 0.03 326 92 102 10 665.68 65.41 327 93 97 4 830.45 1.77 328 94 97 3 897.53 0.80 329 96 99 3 840.16 1.27 330 103 142 39 654.72 21.94 331 105 142 37 664.09 21.21 332 105 151 46 657.58 18.39 333 106 113 7 794.40 1.45 334 106 142 36 664.75 21.23 335 106 144 38 664.70 21.21 336 107 113 6 769.08 14.10 337 107 114 7 769.07 14.10 338 107 117 10 698.00 7.03 339 107 144 37 664.85 21.18 340 107 147 40 664.78 21.21 341 107 148 41 664.77 21.21 342 108 113 5 797.24 2.85 343 108 114 6 799.41 3.94 344 109 114 5 800.99 3.18 345 110 113 3 854.40 0.54 346 110 114 4 813.31 0.71 347 111 114 3 854.87 0.29 348 111 115 4 816.12 0.64 349 111 122 11 665.35 21.14 350 111 152 41 664.97 21.30 351 111 155 44 664.48 21.54 352 111 156 45 664.45 21.55 353 111 160 49 596.20 21.82 354 111 189 78 341.31 1.97 355 112 115 3 855.93 0.24 356 112 116 4 836.46 9.50 357 113 116 3 844.24 5.61 358 113 117 4 827.29 4.92 359 114 117 3 838.43 8.51 360 114 118 4 816.12 0.66 361 114 148 34 665.03 21.27 362 114 151 37 665.02 21.28 363 114 152 38 665.00 21.28 364 114 155 41 664.48 21.55 365 115 118 3 826.09 2.33 366 115 119 4 817.54 1.94 367 115 120 5 782.35 10.34 368 115 121 6 665.41 21.18 369 115 122 7 665.42 21.18 370 115 152 37 665.03 21.28 371 116 119 3 827.27 4.05 372 117 145 28 665.33 21.25 373 117 148 31 665.29 21.25 374 118 148 30 665.29 21.25 375 118 149 31 665.28 21.25 376 118 152 34 665.29 21.25 377 120 124 4 824.07 5.98 378 120 125 5 743.97 13.35 379 121 124 3 854.75 0.21 380 121 125 4 839.06 8.04 381 122 125 3 855.66 0.24 382 122 152 30 665.64 21.14 383 122 156 34 665.30 21.27 384 122 181 59 421.06 0.45 385 122 186 64 362.48 2.78 386 122 189 67 360.29 3.89 387 122 190 68 353.44 1.54 388 123 180 57 421.68 0.25 389 123 181 58 421.19 0.48 390 123 186 63 366.29 4.25 391 124 180 56 421.28 0.46 392 125 149 24 666.96 21.30 393 125 152 27 666.90 21.28 394 125 153 28 666.88 21.28 395 125 180 55 421.26 0.46 396 125 181 56 421.26 0.46 397 126 131 5 806.36 3.42 398 126 153 27 666.92 21.28 399 126 178 52 422.11 0.26 400 126 179 53 422.09 0.26 401 126 180 54 421.20 0.47 402 127 131 4 820.45 3.63 403 127 153 26 666.92 21.27 404 127 156 29 666.90 21.28 405 127 157 30 666.84 21.25 406 127 171 44 433.78 0.24 407 127 174 47 432.07 0.01 408 127 178 51 422.10 0.25 409 127 179 52 422.09 0.26 410 127 181 54 419.13 0.53 411 128 131 3 827.96 4.29 412 128 132 4 823.54 2.08 413 128 157 29 666.85 21.25 414 128 171 43 433.75 0.25 415 128 174 46 431.46 0.29 416 128 178 50 422.12 0.24 417 129 132 3 836.60 2.79 418 129 133 4 823.54 2.08 419 129 171 42 433.75 0.24 420 129 174 45 431.47 0.29 421 129 175 46 430.95 0.26 422 130 133 3 841.74 3.46 423 130 134 4 824.12 2.34 424 130 174 44 431.49 0.28 425 130 175 45 431.43 0.28 426 130 176 46 430.69 0.39 427 130 177 47 423.11 0.07 428 130 178 48 422.24 0.26 429 131 134 3 826.17 3.37 430 131 135 4 824.05 2.38 431 131 153 22 667.04 21.32 432 131 178 47 422.12 0.24 433 132 135 3 826.55 1.12 434 132 136 4 826.00 0.85 435 132 153 21 667.10 21.30 436 132 154 22 667.03 21.32 437 132 157 25 666.98 21.30 438 133 136 3 849.57 1.08 439 133 137 4 830.62 3.15 440 134 137 3 844.33 3.71 441 134 138 4 830.61 3.15 442 135 138 3 838.79 0.95 443 135 139 4 839.34 1.22 444 135 150 15 667.23 21.34 445 135 153 18 667.24 21.34 446 136 139 3 849.01 2.42 447 136 140 4 842.97 0.60 448 137 140 3 847.14 1.48 449 137 141 4 854.55 5.18 450 138 141 3 865.41 1.62 451 138 146 8 667.54 21.24 452 138 147 9 667.52 21.25 453 138 150 12 667.44 21.27 454 139 147 8 667.53 21.25 455 139 150 11 667.49 21.25 456 139 151 12 667.47 21.26 457 139 154 15 667.46 21.25 458 143 146 3 836.32 2.20 459 143 147 4 832.63 3.98 460 144 147 3 838.90 0.90 461 144 148 4 836.32 2.19 462 145 148 3 848.99 4.12 463 145 149 4 846.21 2.73 464 146 149 3 853.17 2.02 465 146 150 4 832.71 4.01 466 147 150 3 845.21 2.24 467 147 151 4 839.40 0.63 468 148 151 3 849.68 2.55 469 148 152 4 839.40 0.63 470 149 152 3 849.69 2.54 471 149 153 4 835.50 1.32 472 150 153 3 843.83 5.48 473 150 154 4 822.32 5.28 474 151 154 3 836.49 1.80 475 151 155 4 820.27 6.30 476 152 156 4 833.96 7.71 477 153 156 3 846.27 1.55 478 153 157 4 833.96 7.71 479 154 157 3 851.76 1.19 480 154 158 4 833.95 7.71 481 155 158 3 851.76 1.19 482 155 159 4 808.22 1.07 483 155 160 5 807.85 0.89 484 156 160 4 745.85 31.89 485 156 161 5 745.86 31.89 486 156 167 11 433.83 0.21 487 156 181 25 402.66 0.51 488 156 186 30 344.88 0.30 489 156 189 33 339.74 1.19 490 157 161 4 771.66 18.98 491 157 171 14 433.58 0.18 492 158 161 3 842.73 2.17 493 158 162 4 814.33 4.19 494 159 162 3 837.63 6.94 495 159 163 4 814.36 4.18 496 160 163 3 832.73 0.73 497 160 164 4 814.35 4.19 498 160 167 7 675.86 18.73 499 160 189 29 335.03 3.54 500 161 164 3 837.35 3.58 501 161 167 6 518.26 40.76 502 161 168 7 433.69 0.20 503 162 238 76 230.78 4.35 504 162 239 77 228.03 3.79 505 163 192 29 309.89 0.64 506 163 237 74 231.90 4.50 507 163 238 75 230.78 4.34 508 163 239 76 227.99 3.75 509 164 167 3 854.25 2.54 510 164 168 4 836.53 1.08 511 164 188 24 346.54 1.23 512 164 189 25 345.51 0.10 513 164 192 28 329.91 2.76 514 164 195 31 318.37 2.13 515 164 236 72 231.97 4.54 516 164 237 73 231.89 4.50 517 164 238 74 230.96 4.43 518 165 168 3 846.61 1.29 519 165 169 4 835.23 1.73 520 165 235 70 231.99 4.54 521 165 236 71 231.97 4.54 522 165 238 73 231.83 4.50 523 166 169 3 854.36 2.58 524 166 170 4 835.23 1.73 525 166 182 16 409.52 0.28 526 166 184 18 369.03 2.87 527 166 185 19 362.13 2.71 528 166 188 22 361.94 2.71 529 166 218 52 286.38 6.52 530 166 236 70 231.99 4.53 531 167 170 3 834.52 1.97 532 167 171 4 830.02 0.99 533 167 185 18 362.01 2.66 534 167 188 21 356.50 5.36 535 167 189 22 347.38 0.81 536 168 171 3 845.82 4.42 537 168 172 4 829.88 0.95 538 169 172 3 843.86 3.84 539 169 173 4 824.81 3.35 540 170 173 3 840.17 1.90 541 170 174 4 824.59 3.24 542 170 182 12 409.73 0.18 543 170 185 15 363.65 3.44 544 171 174 3 837.51 3.23 545 171 175 4 817.70 6.69 546 172 175 3 837.59 3.26 547 174 177 3 832.79 1.64 548 174 178 4 500.22 32.20 549 174 179 5 576.56 77.44 550 174 180 6 681.62 24.91 551 174 181 7 493.12 35.74 552 181 185 4 779.50 20.33 553 181 186 5 774.12 17.64 554 182 185 3 838.78 3.17 555 182 186 4 820.79 5.29 556 183 186 3 838.24 3.44 557 183 187 4 820.79 5.28 558 183 216 33 286.43 6.38 559 184 187 3 830.22 7.88 560 184 188 4 820.78 5.28 561 184 217 33 286.40 6.36 562 184 218 34 286.88 6.27 563 185 188 3 846.72 0.36 564 185 189 4 820.75 5.26 565 186 189 3 841.86 2.75 566 186 190 4 838.11 3.42 567 187 190 3 841.86 2.75 568 187 191 4 828.40 1.44 569 187 215 28 286.41 6.37 570 187 216 29 286.76 6.22 571 187 217 30 287.03 6.19 572 188 191 3 838.12 3.42 573 188 192 4 819.79 5.74 574 188 215 27 287.11 6.02 575 188 217 29 287.23 6.09 576 188 232 44 232.12 4.54 577 188 236 48 232.09 4.53 578 191 194 3 844.26 0.91 579 191 195 4 812.72 0.70 580 191 213 22 289.43 4.88 581 191 215 24 289.54 4.93 582 192 195 3 838.50 3.21 583 192 196 4 821.41 3.62 584 192 237 45 232.09 4.53 585 192 239 47 232.06 4.53 586 192 241 49 222.19 3.54 587 193 196 3 843.62 0.65 588 193 197 4 836.02 2.09 589 193 241 48 222.16 3.56 590 193 246 53 169.97 3.95 591 194 197 3 836.07 2.09 592 194 198 4 836.02 2.09 593 194 213 19 289.81 4.92 594 195 198 3 835.25 2.94 595 195 199 4 831.66 4.26 596 195 209 14 290.41 4.61 597 195 213 18 289.83 4.92 598 195 232 37 234.41 3.65 599 195 237 42 232.11 4.52 600 196 200 4 831.71 4.25 601 196 240 44 229.59 3.79 602 196 241 45 228.46 4.33 603 196 246 50 182.37 6.57 604 197 200 3 849.59 4.58 605 197 201 4 831.69 4.24 606 197 243 46 221.99 3.64 607 197 246 49 183.54 6.61 608 198 201 3 837.57 1.29 609 198 202 4 783.10 23.07 610 198 209 11 290.44 4.63 611 198 210 12 290.51 4.67 612 198 211 13 290.52 4.67 613 198 213 15 290.46 4.73 614 199 202 3 837.57 1.30 615 199 209 10 290.51 4.67 616 199 228 29 288.22 5.50 617 199 233 34 240.26 1.51 618 199 237 38 232.72 4.49 619 199 240 41 232.16 4.54 620 200 203 3 864.52 5.51 621 200 240 40 232.17 4.54 622 200 241 41 232.16 4.54 623 200 242 42 232.10 4.54 624 200 243 43 230.87 4.31 625 200 246 46 228.82 4.15 626 201 205 4 835.50 2.32 627 201 210 9 290.55 4.68 628 202 205 3 820.27 9.94 629 202 206 4 807.35 3.48 630 202 207 5 290.76 4.73 631 202 208 6 290.58 4.66 632 202 209 7 290.56 4.67 633 202 228 26 290.30 4.74 634 205 208 3 827.72 1.48 635 205 210 5 738.34 28.23 636 207 227 20 290.45 4.74 637 207 228 21 290.36 4.74 638 208 226 18 290.56 4.74 639 208 227 19 290.44 4.75 640 209 213 4 855.81 8.90 641 209 225 16 290.62 4.75 642 209 226 17 290.50 4.77 643 209 227 18 290.48 4.77 644 209 228 19 290.49 4.77 645 209 233 24 242.30 2.04 646 212 224 12 291.36 4.54 647 212 225 13 290.84 4.68 648 212 226 14 290.62 4.74 649 213 224 11 291.38 4.54 650 213 225 12 290.97 4.74 651 214 222 8 293.86 3.84 652 214 223 9 293.23 4.08 653 214 224 10 291.38 4.54 654 215 222 7 293.93 3.87 655 215 223 8 293.61 3.90 656 215 225 10 293.22 4.08 657 215 232 17 239.55 0.85 658 216 219 3 886.12 4.15 659 216 220 4 813.31 2.87 660 216 221 5 293.98 3.88 661 216 222 6 293.94 3.88 662 216 223 7 293.92 3.88 663 217 220 3 877.00 5.68 664 217 223 6 301.60 0.07 665 219 222 3 869.49 0.14 666 219 223 4 813.62 2.93 667 225 230 5 297.37 4.36 668 225 232 7 300.73 3.51 669 225 233 8 299.44 2.86 670 227 230 3 871.93 1.40 671 228 233 5 549.63 122.91 672 228 234 6 549.63 122.91 673 229 234 5 549.64 122.92 674 230 233 3 835.80 2.02 675 230 234 4 741.19 27.20 676 231 234 3 758.61 23.73 677 231 235 4 718.92 38.30 678 231 236 5 718.91 38.30 679 232 236 4 718.91 38.30 680 232 237 5 549.57 122.89 681 233 237 4 549.57 122.89 682 235 238 3 979.00 1.02 683 237 240 3 931.22 0.98 684 241 245 4 833.44 5.07 685 241 246 5 755.00 35.73 686 242 245 3 844.85 0.74 687 242 246 4 833.44 5.07 688 243 246 3 823.77 3.21