BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 6 3 842.91 0.73 2 3 7 4 837.88 3.24 3 3 40 37 186.09 0.24 4 4 7 3 839.76 2.31 5 4 8 4 837.88 3.24 6 5 8 3 861.44 2.54 7 5 9 4 829.03 1.23 8 6 9 3 837.93 3.22 9 6 10 4 829.03 1.23 10 6 30 24 188.24 0.29 11 6 32 26 187.91 0.33 12 6 40 34 186.91 0.60 13 7 10 3 829.52 0.99 14 7 11 4 829.03 1.24 15 7 40 33 186.91 0.60 16 8 11 3 828.55 1.89 17 8 12 4 828.12 1.69 18 9 12 3 830.18 1.19 19 9 13 4 829.66 0.93 20 9 30 21 188.34 0.34 21 10 13 3 836.90 0.36 22 10 14 4 838.43 2.90 23 10 26 16 188.32 0.34 24 10 30 20 188.34 0.34 25 10 32 22 188.66 0.17 26 10 40 30 187.02 0.58 27 10 41 31 187.01 0.58 28 10 44 34 187.06 0.54 29 11 14 3 842.91 0.66 30 11 15 4 837.35 3.45 31 11 44 33 186.94 0.59 32 11 49 38 186.79 0.66 33 11 50 39 186.69 0.69 34 11 64 53 162.12 0.23 35 12 15 3 843.08 0.58 36 12 16 4 836.45 3.89 37 13 16 3 844.25 1.32 38 13 17 4 836.44 3.90 39 13 21 8 191.74 1.02 40 13 26 13 189.78 0.38 41 13 29 16 189.80 0.37 42 13 30 17 189.77 0.38 43 14 17 3 833.15 2.82 44 14 21 7 190.12 0.22 45 14 26 12 189.79 0.38 46 14 41 27 187.90 0.32 47 14 50 36 186.82 0.64 48 14 59 45 180.06 0.59 49 14 60 46 171.75 1.04 50 15 60 45 173.30 0.28 51 15 64 49 162.50 0.04 52 17 20 3 892.35 1.04 53 19 59 40 183.00 0.84 54 20 53 33 186.67 0.68 55 20 57 37 183.04 0.85 56 20 58 38 183.00 0.84 57 20 59 39 182.96 0.83 58 21 25 4 813.08 0.29 59 21 26 5 813.01 0.32 60 21 57 36 183.01 0.85 61 21 58 37 182.96 0.83 62 22 25 3 833.39 6.54 63 22 26 4 830.09 8.20 64 22 57 35 182.97 0.83 65 23 26 3 839.08 3.71 66 23 27 4 830.73 7.88 67 23 34 11 191.83 0.72 68 23 37 14 189.45 0.07 69 23 38 15 189.45 0.07 70 23 41 18 189.43 0.07 71 23 52 29 186.68 0.69 72 23 58 35 182.96 0.84 73 24 27 3 821.06 3.04 74 24 28 4 815.10 0.06 75 24 34 10 191.83 0.72 76 25 28 3 829.40 1.13 77 25 29 4 819.23 2.73 78 26 29 3 830.31 1.58 79 26 30 4 814.50 0.38 80 26 37 11 189.84 0.26 81 26 41 15 189.44 0.07 82 27 30 3 828.78 1.57 83 27 31 4 811.77 0.73 84 27 32 5 765.12 11.56 85 27 33 6 441.54 100.19 86 27 34 7 192.65 0.32 87 27 37 10 189.84 0.27 88 28 31 3 828.31 1.79 89 32 36 4 833.22 3.87 90 32 37 5 816.49 4.49 91 32 40 8 799.17 3.77 92 33 36 3 833.27 3.84 93 33 37 4 833.27 3.84 94 34 37 3 833.28 3.83 95 34 38 4 833.26 3.84 96 35 38 3 852.82 3.23 97 35 39 4 833.27 3.84 98 36 39 3 837.81 1.15 99 36 40 4 833.69 3.21 100 37 40 3 839.21 0.05 101 37 41 4 827.75 1.10 102 38 41 3 835.97 1.67 103 38 42 4 820.30 4.83 104 38 52 14 186.93 0.76 105 39 42 3 833.96 0.68 106 39 43 4 840.70 3.99 107 40 43 3 847.10 0.79 108 40 44 4 826.61 3.06 109 41 44 3 826.80 2.96 110 41 45 4 826.60 3.06 111 41 50 9 188.37 1.42 112 41 58 17 182.94 0.84 113 42 45 3 847.07 0.80 114 42 46 4 815.01 7.89 115 43 46 3 826.72 2.04 116 43 47 4 826.49 2.15 117 44 47 3 842.90 0.21 118 44 48 4 813.58 8.60 119 44 49 5 707.94 13.14 120 44 50 6 188.39 1.43 121 45 48 3 826.74 2.05 122 45 50 5 188.40 1.43 123 45 52 7 188.33 1.43 124 45 58 13 183.03 0.83 125 47 63 16 168.00 2.89 126 48 61 13 168.22 2.82 127 48 63 15 167.99 2.88 128 49 60 11 176.47 1.83 129 49 61 12 168.08 2.86 130 49 63 14 167.99 2.87 131 49 64 15 162.32 0.27 132 49 67 18 161.53 0.06 133 50 58 8 183.02 0.83 134 50 59 9 182.91 0.85 135 50 60 10 175.91 1.56 136 50 61 11 168.08 2.85 137 51 58 7 183.02 0.83 138 51 59 8 182.95 0.84 139 51 61 10 177.00 2.10 140 52 56 4 638.59 27.03 141 52 57 5 183.07 0.85 142 52 58 6 183.04 0.84 143 53 56 3 819.65 3.25 144 53 57 4 638.34 26.91 145 53 59 6 183.06 0.85 146 60 64 4 838.18 0.22 147 61 64 3 858.79 0.48 148 62 65 3 847.13 0.94 149 62 66 4 816.48 5.09 150 63 66 3 825.95 0.36 151 63 67 4 811.06 7.81 152 64 67 3 836.43 0.82 153 64 68 4 807.53 1.23 154 65 68 3 836.41 0.83 155 65 69 4 839.50 2.79 156 66 69 3 848.76 0.28 157 66 70 4 839.51 2.79 158 67 70 3 840.51 2.29 159 67 71 4 839.51 2.79 160 68 71 3 842.87 1.11 161 68 72 4 839.50 2.79 162 69 72 3 839.53 2.81 163 69 73 4 830.31 1.80 164 70 73 3 839.98 3.04 165 70 74 4 839.53 2.81 166 71 74 3 847.22 1.02 167 71 75 4 831.13 3.45 168 72 75 3 831.13 3.45 169 75 80 5 825.16 7.24 170 75 244 169 59.76 2.44 171 76 79 3 840.03 0.81 172 76 80 4 825.16 7.24 173 77 80 3 826.96 6.34 174 77 81 4 808.38 1.15 175 77 98 21 267.79 6.28 176 77 100 23 141.13 37.36 177 77 109 32 267.94 6.25 178 77 209 132 67.34 0.49 179 77 240 163 67.30 0.49 180 77 244 167 67.21 0.54 181 78 81 3 817.91 1.82 182 78 82 4 807.38 0.66 183 78 98 20 267.86 6.24 184 78 109 31 267.94 6.25 185 78 110 32 276.43 2.29 186 80 240 160 134.89 33.28 187 80 243 163 67.34 0.50 188 80 244 164 67.33 0.49 189 81 84 3 846.84 4.36 190 81 85 4 810.49 2.11 191 81 98 17 283.25 5.58 192 81 240 159 211.31 6.04 193 82 85 3 832.86 6.08 194 82 86 4 813.14 0.79 195 82 110 28 285.81 6.67 196 83 86 3 828.03 0.61 197 83 87 4 813.25 0.73 198 84 87 3 823.90 2.68 199 84 88 4 825.57 5.42 200 84 236 152 286.26 6.74 201 84 239 155 286.27 6.74 202 84 240 156 286.26 6.74 203 84 243 159 286.28 6.75 204 85 88 3 840.90 6.34 205 85 89 4 837.32 0.45 206 85 96 11 291.36 8.13 207 85 97 12 290.75 8.43 208 85 98 13 290.15 8.56 209 85 110 25 290.98 8.46 210 85 112 27 293.21 7.62 211 85 212 127 286.64 6.81 212 85 240 155 286.59 6.83 213 86 89 3 839.40 0.60 214 86 90 4 837.32 0.45 215 86 112 26 293.57 7.81 216 87 90 3 839.40 0.60 217 87 91 4 837.32 0.44 218 87 236 149 291.31 8.53 219 88 91 3 850.10 2.83 220 88 92 4 832.06 2.16 221 88 96 8 293.87 7.75 222 88 212 124 293.33 7.61 223 88 214 126 294.55 7.41 224 88 233 145 293.25 7.58 225 88 236 148 291.32 8.52 226 89 92 3 840.40 0.67 227 89 93 4 789.61 9.96 228 89 94 5 776.47 3.39 229 89 95 6 682.36 2.26 230 89 96 7 465.73 78.17 231 89 112 23 465.73 78.17 232 89 113 24 499.79 89.04 233 89 114 25 518.07 95.55 234 89 117 28 559.89 80.68 235 91 232 141 294.44 7.54 236 91 235 144 294.44 7.54 237 92 214 122 344.61 0.01 238 92 229 137 326.58 3.56 239 92 232 140 314.99 0.46 240 93 116 23 575.35 73.60 241 93 117 24 575.36 73.61 242 94 117 23 575.36 73.61 243 94 214 120 395.26 0.33 244 94 215 121 395.50 0.28 245 94 216 122 395.51 0.28 246 94 220 126 395.36 0.38 247 94 229 135 387.70 0.10 248 95 112 17 575.62 73.61 249 95 113 18 575.59 73.60 250 95 117 22 575.45 73.56 251 95 118 23 575.44 73.57 252 95 120 25 575.22 73.66 253 95 214 119 395.71 0.18 254 95 215 120 395.72 0.19 255 96 111 15 575.74 73.59 256 96 112 16 575.63 73.60 257 96 212 116 396.05 0.03 258 96 213 117 395.86 0.10 259 96 214 118 395.82 0.13 260 97 108 11 576.27 73.45 261 97 110 13 575.88 73.61 262 97 111 14 575.75 73.60 263 97 120 23 575.54 73.58 264 97 122 25 575.34 73.60 265 97 212 115 396.26 0.05 266 97 213 116 396.03 0.11 267 98 108 10 576.28 73.45 268 98 109 11 576.22 73.47 269 98 110 12 575.90 73.61 270 98 201 103 423.25 13.40 271 98 210 112 420.62 12.00 272 98 211 113 417.44 10.50 273 98 212 114 404.12 3.87 274 99 106 7 576.54 73.49 275 99 107 8 576.38 73.44 276 99 108 9 576.28 73.45 277 99 128 29 569.39 76.23 278 99 135 36 488.66 45.97 279 99 139 40 488.69 45.93 280 99 210 111 423.38 13.33 281 99 211 112 419.88 11.67 282 100 106 6 576.58 73.49 283 100 107 7 576.44 73.47 284 100 109 9 576.32 73.48 285 100 209 109 434.47 18.49 286 100 210 110 430.53 16.89 287 101 105 4 773.39 12.38 288 101 106 5 576.58 73.49 289 101 139 38 489.05 45.77 290 101 143 42 496.19 42.20 291 101 147 46 504.16 38.24 292 101 207 106 435.15 18.64 293 101 208 107 434.76 18.49 294 101 209 108 434.57 18.48 295 102 105 3 830.59 1.21 296 102 107 5 576.69 73.50 297 103 130 27 574.88 73.62 298 104 130 26 574.79 73.64 299 104 147 43 504.26 38.19 300 104 150 46 504.30 38.17 301 104 151 47 504.11 38.19 302 104 154 50 503.16 37.59 303 105 127 22 575.01 73.61 304 105 128 23 574.91 73.64 305 105 130 25 574.29 73.79 306 106 127 21 574.96 73.64 307 106 128 22 574.74 73.69 308 106 130 24 569.36 76.25 309 107 125 18 575.15 73.67 310 107 126 19 575.01 73.66 311 108 122 14 575.50 73.59 312 108 123 15 575.34 73.61 313 108 124 16 575.15 73.67 314 108 125 17 575.10 73.70 315 108 126 18 574.98 73.66 316 108 128 20 573.15 74.43 317 111 120 9 575.56 73.58 318 111 122 11 575.33 73.61 319 113 117 4 730.25 3.26 320 114 117 3 796.78 6.03 321 118 194 76 395.80 0.23 322 118 216 98 395.69 0.17 323 118 217 99 395.73 0.19 324 118 220 102 395.74 0.19 325 120 194 74 395.99 0.07 326 120 215 95 396.00 0.08 327 122 126 4 827.83 3.20 328 123 126 3 854.80 5.46 329 128 133 5 789.76 14.88 330 129 132 3 854.48 4.75 331 129 133 4 829.35 0.50 332 129 160 31 607.93 15.33 333 130 133 3 857.23 3.38 334 130 136 6 613.69 15.95 335 130 137 7 620.29 12.66 336 130 139 9 613.70 15.95 337 130 140 10 613.70 15.96 338 130 151 21 609.81 14.59 339 131 137 6 622.06 13.54 340 131 140 9 616.10 16.52 341 131 151 20 609.83 14.59 342 131 155 24 608.61 15.15 343 131 156 25 608.60 15.15 344 131 157 26 607.93 15.21 345 131 158 27 608.26 15.31 346 131 160 29 608.21 15.37 347 131 164 33 607.58 15.38 348 131 171 40 606.54 15.19 349 132 160 28 608.18 15.37 350 132 164 32 607.50 15.35 351 132 189 57 581.76 18.00 352 132 190 58 577.77 19.37 353 133 136 3 815.94 1.60 354 133 137 4 819.11 0.01 355 133 186 53 582.38 17.95 356 134 137 3 830.69 5.42 357 134 138 4 819.34 0.12 358 134 184 50 582.45 17.92 359 134 185 51 582.42 17.93 360 134 186 52 582.38 17.95 361 134 196 62 513.23 17.91 362 134 197 63 512.92 17.93 363 134 198 64 511.81 18.04 364 134 213 79 506.64 16.46 365 135 138 3 825.28 2.90 366 135 139 4 823.06 1.73 367 135 198 63 511.99 18.13 368 135 211 76 506.92 16.48 369 135 213 78 506.63 16.46 370 136 139 3 841.63 5.90 371 136 140 4 836.53 8.46 372 137 140 3 842.00 5.73 373 137 141 4 832.25 0.85 374 137 174 37 606.46 15.15 375 137 175 38 606.45 15.15 376 137 178 41 604.69 15.14 377 137 184 47 582.47 17.91 378 137 185 48 582.46 17.92 379 137 186 49 582.44 17.92 380 137 189 52 582.03 18.10 381 138 141 3 846.49 3.75 382 138 142 4 836.46 0.35 383 138 178 40 604.78 15.09 384 138 184 46 582.46 17.91 385 138 198 60 512.01 18.13 386 138 199 61 511.78 18.05 387 138 200 62 511.69 18.09 388 139 142 3 850.81 1.59 389 139 143 4 824.62 2.62 390 139 208 69 507.99 16.47 391 139 210 71 507.51 16.62 392 139 211 72 506.94 16.47 393 140 143 3 829.07 0.94 394 140 148 8 615.97 17.60 395 140 151 11 609.92 14.59 396 140 171 31 607.25 15.48 397 140 175 35 606.52 15.19 398 141 175 34 606.46 15.15 399 141 178 37 604.69 15.14 400 141 179 38 604.59 15.16 401 142 179 37 604.66 15.13 402 142 200 58 511.68 18.05 403 142 202 60 510.36 17.43 404 142 208 66 508.22 16.54 405 143 147 4 824.86 5.88 406 143 148 5 785.66 7.67 407 143 208 65 508.09 16.49 408 144 147 3 832.48 2.07 409 144 148 4 824.86 5.88 410 144 207 63 508.37 16.61 411 145 148 3 840.90 2.13 412 145 149 4 824.86 5.88 413 146 149 3 840.90 2.14 414 146 150 4 824.86 5.88 415 147 150 3 831.95 2.31 416 147 151 4 820.47 8.04 417 147 207 60 508.33 16.59 418 148 151 3 830.93 1.80 419 148 152 4 817.06 0.48 420 148 172 24 607.26 15.47 421 148 175 27 607.20 15.46 422 149 152 3 847.51 3.44 423 149 153 4 836.89 8.46 424 150 153 3 836.90 8.46 425 150 154 4 832.21 10.80 426 151 154 3 854.68 0.42 427 151 155 4 810.34 2.85 428 152 155 3 860.06 2.27 429 152 168 16 607.35 15.49 430 152 171 19 607.38 15.46 431 152 172 20 607.34 15.49 432 157 165 8 608.84 15.84 433 157 166 9 608.62 15.83 434 157 167 10 608.62 15.83 435 157 168 11 608.62 15.83 436 157 171 14 608.61 15.84 437 158 164 6 609.18 15.92 438 158 166 8 608.68 15.85 439 159 164 5 609.23 15.95 440 159 166 7 608.75 15.88 441 160 164 4 776.75 5.05 442 160 189 29 582.28 18.00 443 160 190 30 582.06 18.05 444 161 164 3 817.91 2.25 445 161 188 27 582.37 17.99 446 161 189 28 582.13 18.08 447 161 192 31 581.95 18.01 448 162 192 30 582.03 18.05 449 163 187 24 582.39 17.99 450 163 188 25 582.36 17.98 451 163 189 26 582.13 18.07 452 164 189 25 582.10 18.08 453 165 170 5 765.82 10.83 454 165 171 6 624.71 16.71 455 165 189 24 582.13 18.08 456 167 170 3 803.33 5.58 457 167 171 4 802.51 5.17 458 168 171 3 837.16 2.19 459 168 172 4 802.60 5.21 460 169 172 3 834.54 0.53 461 169 173 4 818.73 1.86 462 170 173 3 838.11 1.26 463 170 174 4 818.73 1.86 464 171 174 3 832.37 4.12 465 171 175 4 826.64 2.10 466 172 175 3 833.79 1.22 467 172 176 4 826.64 2.10 468 173 176 3 829.45 0.70 469 173 177 4 828.30 1.27 470 174 177 3 830.58 0.96 471 174 178 4 819.43 3.17 472 175 178 3 828.36 0.14 473 175 179 4 818.17 3.80 474 176 179 3 827.22 0.72 475 176 180 4 818.18 3.79 476 177 180 3 829.36 1.79 477 177 181 4 816.53 3.02 478 178 181 3 816.51 3.02 479 178 182 4 816.50 3.01 480 178 183 5 719.00 2.70 481 178 184 6 582.52 17.93 482 181 234 53 450.49 27.79 483 182 234 52 449.19 28.41 484 183 197 14 513.34 17.88 485 183 198 15 513.23 17.92 486 183 199 16 512.78 17.87 487 183 226 43 451.06 27.64 488 183 230 47 450.52 27.80 489 183 234 51 448.42 28.70 490 184 197 13 513.30 17.88 491 184 198 14 512.95 17.93 492 185 189 4 874.20 3.52 493 185 195 10 513.67 17.92 494 185 196 11 513.43 17.91 495 185 197 12 513.31 17.88 496 185 226 41 451.10 27.62 497 186 189 3 844.90 3.98 498 186 190 4 838.11 0.59 499 186 195 9 513.62 17.89 500 186 196 10 513.42 17.91 501 187 195 8 513.68 17.88 502 188 191 3 825.38 0.89 503 188 192 4 755.02 20.65 504 188 193 5 628.01 24.89 505 188 194 6 546.13 25.46 506 188 195 7 513.73 17.90 507 188 218 30 502.90 16.14 508 191 194 3 851.55 0.15 509 193 217 24 504.57 16.18 510 193 218 25 503.05 16.08 511 194 215 21 506.19 16.39 512 194 216 22 505.84 16.36 513 194 217 23 504.59 16.19 514 194 218 24 503.01 16.09 515 195 215 20 506.18 16.39 516 195 216 21 505.98 16.41 517 195 218 23 501.28 15.88 518 195 221 26 484.85 19.76 519 195 226 31 458.64 23.88 520 196 214 18 506.37 16.41 521 196 215 19 506.05 16.45 522 196 221 25 483.88 20.25 523 197 213 16 506.79 16.46 524 197 214 17 506.62 16.46 525 197 221 24 477.38 23.33 526 197 226 29 450.62 27.86 527 197 229 32 450.02 28.05 528 197 230 33 449.80 28.16 529 197 233 36 448.54 28.76 530 198 212 14 506.94 16.48 531 198 213 15 506.72 16.50 532 198 233 35 448.41 28.70 533 199 211 12 507.14 16.41 534 199 212 13 507.08 16.43 535 199 233 34 448.42 28.70 536 199 234 35 448.32 28.73 537 199 237 38 407.86 18.15 538 200 208 8 508.36 16.54 539 200 210 10 508.00 16.46 540 200 211 11 507.31 16.47 541 200 237 37 407.89 18.17 542 201 208 7 508.46 16.59 543 201 209 8 508.41 16.56 544 201 210 9 508.19 16.53 545 201 211 10 508.01 16.46 546 201 212 11 508.00 16.46 547 201 240 39 363.53 0.48 548 201 241 40 362.39 0.59 549 201 244 43 361.67 0.32 550 202 206 4 508.57 16.58 551 202 207 5 508.56 16.59 552 202 208 6 508.50 16.60 553 202 209 7 508.49 16.59 554 202 241 39 362.80 0.40 555 202 244 42 361.68 0.32 556 202 246 44 355.15 0.31 557 203 206 3 814.02 3.17 558 203 207 4 516.39 20.50 559 203 209 6 508.52 16.60 560 203 244 41 361.68 0.32 561 203 246 43 360.01 0.35 562 204 244 40 362.31 0.56 563 204 245 41 361.16 0.14 564 204 246 42 360.43 0.14 565 206 246 40 361.02 0.24 566 209 244 35 361.62 0.35 567 212 233 21 448.32 28.73 568 212 237 25 406.63 18.24 569 213 233 20 448.34 28.73 570 214 221 7 700.74 14.40 571 214 229 15 449.05 28.53 572 214 232 18 448.51 28.78 573 214 233 19 448.44 28.81 574 215 221 6 771.39 3.72 575 216 221 5 791.32 2.56 576 217 220 3 846.62 4.85 577 217 221 4 831.94 2.76 578 218 221 3 848.73 1.35 579 219 222 3 898.95 2.15 580 220 225 5 811.63 2.60 581 221 225 4 812.02 2.42 582 221 226 5 791.46 7.46 583 221 229 8 753.34 1.52 584 222 225 3 820.12 2.56 585 222 226 4 815.90 0.48 586 223 226 3 840.52 2.21 587 223 227 4 822.58 2.85 588 224 227 3 830.00 2.38 589 224 228 4 818.21 0.07 590 225 228 3 823.16 2.54 591 225 229 4 822.80 2.66 592 226 229 3 837.43 9.69 593 226 230 4 839.18 8.81 594 227 230 3 845.84 5.49 595 227 231 4 840.54 8.13 596 228 231 3 856.00 1.22 597 228 232 4 840.57 8.12 598 229 232 3 846.66 5.90 599 229 233 4 840.59 8.10 600 230 233 3 826.31 0.96 601 230 234 4 828.14 1.88 602 231 234 3 839.64 3.87 603 231 235 4 828.98 1.46 604 232 235 3 834.11 0.14 605 232 236 4 828.99 1.46 606 233 236 3 848.24 5.12 607 233 237 4 821.07 1.46 608 234 237 3 825.98 0.99 609 234 238 4 825.97 0.99 610 235 238 3 845.64 5.55 611 235 239 4 831.21 1.61 612 236 239 3 841.57 3.58 613 236 240 4 831.20 1.61 614 237 240 3 834.38 0.02 615 237 241 4 831.19 1.61 616 238 241 3 835.66 9.45 617 238 242 4 820.76 2.02 618 239 242 3 822.46 2.88 619 239 243 4 828.19 1.71 620 240 243 3 829.91 0.84 621 240 244 4 826.12 2.74 622 241 244 3 825.81 2.90 623 241 245 4 818.31 0.85 624 241 246 5 764.11 11.43 625 242 245 3 818.19 1.03 626 242 246 4 779.33 8.00