BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 204 201 106.46 0.10 2 3 205 202 106.31 0.04 3 3 224 221 106.40 0.08 4 3 227 224 106.09 0.03 5 4 34 30 736.12 35.95 6 4 35 31 736.11 35.95 7 4 36 32 736.09 35.95 8 4 37 33 736.07 35.95 9 4 38 34 736.06 35.95 10 4 205 201 106.47 0.09 11 5 37 32 736.09 35.95 12 5 38 33 736.09 35.95 13 5 221 216 106.34 0.04 14 5 227 222 106.36 0.05 15 5 247 242 17.55 0.93 16 6 38 32 736.09 35.95 17 6 40 34 736.08 35.95 18 6 90 84 286.93 82.43 19 6 124 118 212.52 51.06 20 6 161 155 195.72 42.68 21 6 205 199 109.47 0.41 22 6 207 201 106.45 0.08 23 6 227 221 107.92 0.62 24 6 228 222 106.34 0.04 25 6 229 223 106.34 0.04 26 7 37 30 736.12 35.95 27 7 38 31 736.10 35.95 28 7 39 32 736.08 35.95 29 7 40 33 736.08 35.95 30 7 229 222 106.38 0.06 31 7 240 233 47.34 1.41 32 7 243 236 44.56 0.31 33 7 244 237 37.22 0.14 34 7 247 240 20.43 2.33 35 8 40 32 736.07 35.95 36 8 42 34 736.06 35.95 37 8 229 221 108.59 0.29 38 8 230 222 106.33 0.04 39 8 231 223 106.12 0.04 40 9 40 31 736.11 35.95 41 9 41 32 736.07 35.95 42 9 42 33 736.07 35.95 43 9 231 222 106.34 0.04 44 9 235 226 80.24 0.13 45 9 240 231 50.26 0.06 46 10 42 32 736.09 35.95 47 10 64 54 563.09 1.32 48 10 92 82 287.31 82.09 49 10 95 85 279.08 80.44 50 10 230 220 109.61 0.34 51 10 231 221 108.84 0.11 52 10 232 222 106.12 0.04 53 10 235 225 85.17 0.31 54 11 14 3 867.37 3.00 55 11 20 9 737.20 35.97 56 11 23 12 737.19 35.97 57 11 24 13 737.19 35.97 58 11 27 16 737.12 35.99 59 11 41 30 736.14 35.95 60 11 42 31 736.10 35.95 61 11 43 32 736.08 35.95 62 11 51 40 574.57 2.31 63 11 64 53 563.18 1.31 64 11 235 224 101.14 0.38 65 11 236 225 84.48 0.65 66 12 44 32 736.08 35.95 67 12 64 52 563.09 1.32 68 12 95 83 282.18 79.18 69 12 97 85 258.55 65.64 70 12 232 220 109.66 0.36 71 13 44 31 736.10 35.95 72 14 44 30 736.10 35.95 73 14 236 222 105.71 0.06 74 15 19 4 837.88 0.95 75 15 20 5 837.88 0.95 76 15 47 32 735.85 35.94 77 16 19 3 843.88 2.03 78 16 20 4 843.87 2.04 79 16 47 31 735.86 35.95 80 17 20 3 854.63 3.27 81 17 21 4 843.94 2.08 82 17 50 33 698.58 23.86 83 18 21 3 847.59 0.26 84 18 22 4 843.96 2.09 85 19 22 3 856.51 0.94 86 19 23 4 848.49 4.39 87 20 23 3 849.05 4.67 88 20 24 4 848.49 4.40 89 20 47 27 735.14 35.63 90 20 50 30 663.88 30.21 91 20 51 31 581.39 5.73 92 20 54 34 573.50 2.62 93 21 24 3 856.71 0.85 94 21 25 4 856.13 0.57 95 21 54 33 571.12 1.43 96 22 25 3 863.71 4.35 97 22 26 4 856.14 0.57 98 23 26 3 863.05 2.31 99 23 27 4 856.12 0.56 100 23 235 212 103.14 0.76 101 23 236 213 90.55 2.13 102 23 237 214 81.67 0.78 103 23 238 215 80.27 0.16 104 24 27 3 861.91 3.46 105 24 28 4 851.03 1.97 106 24 54 30 568.76 0.92 107 24 55 31 567.53 0.35 108 25 28 3 873.39 2.27 109 25 29 4 861.91 3.46 110 26 29 3 876.84 0.55 111 26 238 212 76.29 1.70 112 26 241 215 59.98 1.02 113 27 30 3 871.05 2.68 114 27 41 14 736.19 35.91 115 27 55 28 567.47 0.32 116 27 59 32 566.43 0.40 117 27 235 208 93.86 3.56 118 27 237 210 80.33 0.14 119 27 240 213 54.59 1.24 120 27 241 214 47.23 1.45 121 28 54 26 567.60 0.33 122 28 55 27 567.47 0.32 123 28 56 28 567.43 0.32 124 28 58 30 566.93 0.42 125 28 59 31 566.71 0.52 126 30 58 28 565.19 0.84 127 30 241 211 46.52 1.03 128 30 244 214 45.87 0.70 129 30 245 215 45.08 0.42 130 31 248 217 37.09 0.10 131 32 39 7 736.51 35.88 132 32 58 26 564.44 1.02 133 32 59 27 564.44 1.02 134 32 248 216 31.98 0.25 135 33 36 3 858.77 0.35 136 33 37 4 833.67 1.32 137 34 37 3 849.44 2.52 138 34 58 24 563.09 1.28 139 37 244 207 39.94 0.04 140 37 247 210 25.00 0.05 141 37 248 211 17.53 0.92 142 38 205 167 109.56 0.41 143 39 59 20 563.25 1.32 144 39 60 21 563.25 1.32 145 39 244 205 37.23 0.14 146 40 60 20 563.25 1.32 147 40 61 21 563.23 1.33 148 40 62 22 563.23 1.33 149 40 90 50 285.43 83.22 150 41 51 10 567.85 0.79 151 41 55 14 566.83 0.57 152 41 59 18 564.39 1.02 153 41 60 19 564.04 0.94 154 41 61 20 563.24 1.33 155 41 62 21 563.23 1.33 156 42 62 20 563.22 1.33 157 42 63 21 563.19 1.31 158 42 64 22 563.14 1.29 159 42 90 48 287.32 82.28 160 42 91 49 287.47 82.21 161 42 92 50 287.42 82.19 162 42 124 82 238.18 57.76 163 43 47 4 824.71 4.00 164 43 48 5 737.78 36.59 165 43 51 8 573.50 2.71 166 43 62 19 564.07 0.95 167 43 64 21 563.20 1.32 168 44 47 3 853.00 2.04 169 44 48 4 847.65 4.71 170 44 64 20 563.18 1.32 171 45 48 3 847.66 4.71 172 47 50 3 852.88 2.68 173 47 51 4 842.91 1.28 174 48 51 3 851.12 1.80 175 48 52 4 843.20 1.36 176 48 61 13 565.16 0.81 177 48 62 14 564.67 0.93 178 48 63 15 564.54 0.94 179 48 88 40 289.07 81.47 180 49 52 3 856.39 1.06 181 49 53 4 850.20 2.04 182 50 53 3 860.94 2.90 183 50 54 4 854.71 0.21 184 51 54 3 856.91 0.69 185 51 55 4 854.70 0.22 186 52 55 3 856.29 1.01 187 52 56 4 854.90 0.31 188 52 57 5 846.38 0.11 189 52 59 7 802.94 3.77 190 52 60 8 738.29 36.08 191 52 61 9 565.62 1.03 192 55 59 4 819.71 3.29 193 55 61 6 738.19 36.09 194 56 59 3 872.67 2.53 195 59 244 185 41.21 0.59 196 60 89 29 285.46 83.20 197 60 90 30 285.45 83.20 198 61 89 28 286.07 82.90 199 61 90 29 286.06 82.89 200 62 88 26 287.16 82.43 201 62 90 28 285.45 83.20 202 63 78 15 295.06 79.06 203 63 83 20 293.73 79.28 204 63 88 25 288.72 81.67 205 63 90 27 287.35 82.31 206 63 91 28 287.31 82.28 207 63 92 29 287.43 82.21 208 64 92 28 287.42 82.19 209 65 92 27 291.58 80.15 210 65 98 33 262.46 63.70 211 65 112 47 251.61 57.56 212 66 78 12 295.69 78.77 213 66 79 13 295.11 79.05 214 66 80 14 295.07 79.06 215 66 91 25 293.58 79.23 216 66 93 27 293.61 79.16 217 66 112 46 251.58 57.55 218 66 116 50 251.30 57.42 219 67 77 10 296.00 78.75 220 67 78 11 295.78 78.78 221 67 79 12 295.12 79.05 222 67 112 45 251.69 57.52 223 68 108 40 251.81 57.58 224 68 111 43 251.71 57.52 225 68 112 44 251.71 57.52 226 69 74 5 647.00 32.90 227 69 79 10 296.38 78.62 228 70 74 4 888.45 0.19 229 70 79 9 296.38 78.61 230 70 81 11 296.42 78.64 231 71 74 3 961.65 2.00 232 71 79 8 296.43 78.64 233 71 81 10 296.43 78.63 234 71 82 11 296.43 78.63 235 74 77 3 880.76 2.50 236 78 82 4 846.73 2.01 237 79 82 3 856.49 2.88 238 79 83 4 825.81 0.68 239 80 83 3 855.49 2.37 240 80 84 4 855.49 2.37 241 80 115 35 339.01 13.58 242 80 116 36 338.08 14.04 243 80 119 39 355.05 5.46 244 80 121 41 339.39 12.94 245 81 84 3 855.49 2.37 246 81 85 4 855.49 2.37 247 81 119 38 355.06 5.46 248 82 85 3 849.36 1.62 249 82 86 4 831.50 3.46 250 83 86 3 845.17 0.49 251 83 87 4 831.50 3.46 252 83 88 5 483.67 6.41 253 83 121 38 379.91 7.85 254 84 87 3 843.45 2.51 255 84 119 35 380.85 7.45 256 84 120 36 388.78 11.42 257 84 121 37 380.51 7.55 258 88 121 33 409.31 26.07 259 89 122 33 407.75 26.84 260 90 122 32 407.64 26.91 261 90 124 34 407.64 26.91 262 90 161 71 407.66 26.91 263 91 116 25 413.76 23.91 264 91 121 30 409.39 26.11 265 91 122 31 407.85 26.87 266 91 123 32 407.69 26.93 267 91 124 33 407.67 26.92 268 92 95 3 917.72 0.17 269 92 124 32 407.62 26.96 270 92 126 34 407.60 26.95 271 92 230 138 156.96 23.11 272 93 109 16 414.16 23.75 273 93 112 19 414.06 23.84 274 93 113 20 414.10 23.79 275 93 116 23 414.06 23.84 276 93 123 30 407.94 26.84 277 93 124 31 407.81 26.88 278 93 125 32 407.62 26.96 279 93 126 33 407.61 26.95 280 94 99 5 799.02 12.15 281 94 109 15 414.16 23.75 282 94 126 32 407.62 26.95 283 95 98 3 849.93 0.42 284 95 99 4 830.38 1.73 285 95 126 31 407.62 26.96 286 95 165 70 407.40 27.02 287 95 230 135 157.52 23.01 288 96 99 3 855.47 10.62 289 96 100 4 827.68 0.39 290 96 165 69 407.13 27.17 291 96 167 71 400.91 30.02 292 96 170 74 368.88 26.09 293 97 100 3 830.37 1.73 294 97 101 4 825.89 1.27 295 97 102 5 744.60 39.37 296 98 102 4 668.50 77.31 297 98 103 5 668.50 77.31 298 98 104 6 474.63 3.81 299 99 103 4 580.19 55.73 300 99 104 5 474.52 3.87 301 99 106 7 474.51 3.87 302 99 109 10 414.79 23.90 303 99 126 27 407.85 26.86 304 99 127 28 407.63 26.96 305 99 146 47 407.98 26.89 306 104 108 4 825.87 1.47 307 104 112 8 750.53 30.23 308 105 108 3 848.60 1.88 309 105 109 4 838.77 3.10 310 106 109 3 842.45 4.95 311 106 110 4 838.80 3.12 312 106 146 40 412.23 24.77 313 106 149 43 413.21 24.48 314 107 110 3 852.48 0.07 315 107 111 4 838.80 3.12 316 108 111 3 842.53 1.26 317 108 112 4 838.78 3.11 318 109 112 3 845.98 2.98 319 109 113 4 842.09 1.46 320 109 125 16 412.85 24.54 321 109 150 41 414.99 23.68 322 110 113 3 844.38 2.14 323 110 114 4 833.01 3.08 324 110 149 39 414.97 23.68 325 110 150 40 414.97 23.68 326 110 152 42 462.71 3.30 327 110 153 43 462.69 3.31 328 111 114 3 845.78 3.08 329 111 115 4 833.24 3.19 330 112 115 3 842.28 1.33 331 112 116 4 835.01 2.32 332 113 116 3 849.56 2.31 333 113 117 4 835.01 2.32 334 113 123 10 415.00 23.69 335 113 125 12 414.95 23.70 336 113 150 37 415.41 23.73 337 113 153 40 470.46 7.16 338 113 160 47 462.47 3.29 339 114 117 3 870.07 3.35 340 114 118 4 845.92 3.14 341 114 153 39 467.36 5.61 342 115 118 3 853.20 0.50 343 115 119 4 853.19 0.50 344 116 119 3 857.82 0.83 345 116 120 4 845.74 3.22 346 116 121 5 732.47 25.05 347 117 120 3 840.06 6.07 348 117 121 4 766.41 22.05 349 117 153 36 639.64 26.27 350 117 154 37 638.13 25.51 351 117 155 38 683.09 25.05 352 117 160 43 582.28 48.33 353 122 159 37 718.66 27.60 354 123 159 36 718.80 27.62 355 123 160 37 718.80 27.64 356 123 161 38 718.79 27.64 357 124 161 37 718.81 27.64 358 124 163 39 718.79 27.64 359 125 150 25 719.19 27.60 360 125 160 35 718.89 27.64 361 125 161 36 718.86 27.65 362 125 162 37 718.81 27.64 363 125 163 38 718.83 27.64 364 126 163 37 718.84 27.65 365 126 165 39 718.79 27.65 366 126 230 104 158.21 22.66 367 127 143 16 719.56 27.64 368 127 146 19 719.37 27.57 369 127 147 20 719.37 27.57 370 127 150 23 719.37 27.57 371 127 162 35 718.90 27.64 372 127 163 36 718.87 27.66 373 127 164 37 718.84 27.66 374 127 165 38 718.83 27.66 375 127 188 61 248.25 21.98 376 128 143 15 719.58 27.64 377 128 146 18 719.49 27.59 378 128 164 36 718.85 27.66 379 128 165 37 718.84 27.66 380 128 167 39 718.73 27.68 381 129 133 4 774.85 24.86 382 129 134 5 769.19 27.69 383 129 139 10 719.63 27.62 384 129 142 13 719.61 27.63 385 129 143 14 719.60 27.62 386 129 164 35 718.90 27.64 387 129 167 38 718.79 27.67 388 129 168 39 718.75 27.69 389 130 133 3 852.75 1.10 390 130 134 4 826.00 0.70 391 130 167 37 718.77 27.69 392 130 168 38 718.71 27.68 393 131 134 3 839.97 2.62 394 131 135 4 826.00 0.70 395 131 168 37 718.73 27.71 396 131 172 41 683.10 23.80 397 131 174 43 642.70 29.21 398 132 136 4 832.46 3.58 399 133 136 3 850.38 5.11 400 133 137 4 825.48 0.09 401 133 138 5 725.65 30.45 402 133 139 6 719.99 27.62 403 133 142 9 719.96 27.62 404 134 137 3 832.46 3.58 405 134 138 4 758.89 22.57 406 134 139 5 726.19 30.18 407 134 164 30 719.07 27.64 408 134 168 34 718.87 27.66 409 134 180 46 306.37 11.78 410 138 141 3 855.65 2.46 411 138 142 4 842.86 8.84 412 139 142 3 855.51 2.52 413 139 143 4 842.88 8.84 414 139 184 45 248.52 22.03 415 140 143 3 855.51 2.52 416 140 144 4 828.21 0.43 417 140 187 47 248.38 21.96 418 141 144 3 862.32 1.64 419 141 145 4 846.35 8.29 420 142 145 3 864.01 0.79 421 142 146 4 845.69 8.62 422 143 146 3 862.68 0.12 423 143 147 4 849.10 6.93 424 143 164 21 718.93 27.64 425 143 184 41 248.39 21.96 426 143 188 45 248.27 21.98 427 143 191 48 248.25 21.98 428 144 147 3 862.70 0.13 429 144 148 4 851.83 5.57 430 144 191 47 248.21 21.96 431 145 148 3 853.68 4.65 432 145 149 4 854.04 4.46 433 146 149 3 862.96 0.06 434 146 150 4 862.97 0.06 435 147 150 3 865.38 1.50 436 147 151 4 837.39 3.85 437 147 191 44 248.20 21.96 438 148 151 3 862.74 0.18 439 148 195 47 247.41 21.94 440 150 153 3 861.36 0.93 441 150 154 4 852.25 2.95 442 150 162 12 718.90 27.66 443 151 154 3 863.00 1.30 444 151 195 44 246.96 22.12 445 151 196 45 246.53 22.23 446 154 157 3 880.25 0.70 447 154 160 6 718.96 27.67 448 154 204 50 226.62 31.53 449 156 159 3 865.85 3.65 450 157 192 35 247.47 21.78 451 157 196 39 246.59 22.19 452 157 200 43 226.82 31.45 453 157 203 46 226.62 31.54 454 157 204 47 226.56 31.54 455 158 161 3 923.47 1.29 456 158 203 45 226.62 31.54 457 158 205 47 226.56 31.54 458 160 204 44 226.53 31.55 459 161 205 44 226.05 31.49 460 161 207 46 225.27 31.64 461 162 188 26 248.19 21.98 462 162 192 30 247.97 22.00 463 162 204 42 226.62 31.55 464 162 205 43 226.29 31.58 465 162 206 44 224.90 31.67 466 162 207 45 224.38 31.77 467 163 207 44 220.50 33.70 468 163 209 46 216.00 35.47 469 163 230 67 157.56 22.98 470 164 184 20 248.29 22.00 471 164 185 21 248.26 21.99 472 164 188 24 248.22 21.98 473 164 207 43 220.82 33.87 474 164 209 45 216.25 35.59 475 165 170 5 835.03 5.90 476 166 169 3 874.80 5.80 477 166 170 4 835.04 5.90 478 166 208 42 225.09 31.73 479 166 209 43 216.64 35.64 480 166 210 44 215.75 36.02 481 167 170 3 848.50 0.83 482 167 172 5 830.35 3.56 483 168 172 4 830.35 3.56 484 168 174 6 683.02 9.04 485 168 175 7 675.13 5.10 486 168 176 8 534.52 65.20 487 168 180 12 279.32 25.21 488 169 172 3 865.13 2.36 489 169 174 5 492.99 1.51 490 169 175 6 443.20 19.57 491 169 176 7 290.28 27.20 492 170 210 40 214.30 36.72 493 176 180 4 787.99 33.32 494 176 208 32 226.90 31.72 495 177 180 3 853.56 0.53 496 177 181 4 844.49 5.07 497 178 181 3 846.08 4.28 498 178 182 4 850.70 1.97 499 178 219 41 166.86 22.33 500 178 220 42 167.12 22.73 501 178 223 45 166.25 22.45 502 179 182 3 850.70 1.97 503 179 183 4 850.70 1.97 504 180 184 4 854.05 0.76 505 181 184 3 857.75 1.09 506 181 185 4 846.10 2.71 507 181 208 27 226.90 31.71 508 181 220 39 171.19 20.14 509 181 226 45 165.37 22.57 510 182 185 3 858.59 3.52 511 182 186 4 844.58 3.48 512 182 223 41 165.43 22.55 513 182 224 42 165.43 22.55 514 182 225 43 165.37 22.57 515 182 226 44 165.34 22.55 516 183 186 3 853.49 0.97 517 183 187 4 844.59 3.47 518 184 187 3 855.91 2.12 519 184 188 4 843.59 1.55 520 185 188 3 853.14 0.72 521 185 189 4 848.50 1.22 522 185 206 21 227.03 31.71 523 185 207 22 227.03 31.71 524 185 208 23 226.94 31.70 525 185 225 40 165.08 22.48 526 185 226 41 164.92 22.50 527 186 189 3 857.12 1.36 528 186 190 4 857.12 1.36 529 186 225 39 165.13 22.50 530 187 190 3 862.77 1.46 531 187 191 4 857.12 1.36 532 188 191 3 855.39 2.23 533 188 192 4 855.39 2.23 534 188 206 18 227.03 31.71 535 189 192 3 846.78 2.29 536 189 193 4 835.91 2.21 537 189 201 12 231.13 30.06 538 189 204 15 230.69 29.93 539 189 206 17 227.05 31.71 540 189 225 36 164.98 22.50 541 189 227 38 164.94 22.50 542 190 193 3 844.14 5.37 543 190 194 4 834.51 1.51 544 191 194 3 850.82 5.00 545 191 195 4 837.12 1.85 546 192 195 3 838.18 2.39 547 192 196 4 769.62 24.57 548 192 197 5 768.02 25.37 549 192 200 8 294.01 32.59 550 192 201 9 235.59 27.84 551 192 204 12 230.75 29.89 552 193 197 4 416.66 25.99 553 193 198 5 284.38 24.84 554 193 201 8 241.65 30.82 555 196 200 4 817.73 0.48 556 197 200 3 851.94 1.04 557 197 201 4 850.99 0.45 558 198 201 3 857.65 1.81 559 198 202 4 850.99 0.45 560 199 202 3 854.96 0.46 561 199 203 4 851.67 0.10 562 200 203 3 862.08 4.02 563 200 204 4 850.56 0.24 564 201 204 3 857.68 1.81 565 204 227 23 162.38 23.58 566 205 227 22 162.19 23.67 567 206 225 19 163.37 23.23 568 206 226 20 162.58 23.62 569 206 227 21 162.24 23.66 570 207 227 20 161.97 23.56 571 207 228 21 159.80 23.27 572 207 229 22 159.63 23.27 573 208 212 4 871.60 0.00 574 208 220 12 163.69 23.75 575 208 226 18 162.59 23.63 576 208 228 20 161.30 23.59 577 208 229 21 159.74 23.21 578 208 230 22 159.72 23.23 579 209 230 21 159.69 23.23 580 211 216 5 845.29 3.73 581 212 216 4 845.29 3.73 582 212 217 5 743.21 25.39 583 212 220 8 379.73 60.86 584 212 229 17 159.86 23.23 585 212 231 19 159.77 23.21 586 212 234 22 158.27 22.64 587 212 243 31 47.24 1.39 588 213 216 3 859.47 2.81 589 213 217 4 819.65 3.86 590 213 234 21 158.41 22.58 591 213 243 30 47.27 1.40 592 214 217 3 863.34 0.87 593 214 239 25 112.42 33.93 594 214 242 28 47.29 1.42 595 214 243 29 47.14 1.34 596 216 219 3 868.58 3.49 597 216 220 4 840.81 1.35 598 217 220 3 847.73 2.44 599 217 221 4 840.81 1.35 600 217 229 12 164.67 22.88 601 217 243 26 46.11 0.92 602 217 246 29 36.29 0.78 603 218 221 3 877.80 3.91 604 218 222 4 843.18 0.17 605 218 246 28 36.16 0.71 606 219 222 3 877.80 3.90 607 219 223 4 845.30 1.23 608 220 223 3 872.52 3.10 609 220 226 6 358.87 18.55 610 220 229 9 165.34 22.58 611 221 226 5 372.00 11.98 612 221 227 6 352.37 21.80 613 221 228 7 165.44 22.62 614 221 229 8 165.46 22.63 615 221 246 25 36.11 0.70 616 221 247 26 30.03 1.85 617 223 226 3 845.86 3.72 618 229 243 14 44.68 0.28 619 231 234 3 875.88 1.96 620 231 235 4 755.79 34.04 621 231 240 9 111.48 30.63 622 231 243 12 46.85 1.26 623 232 235 3 870.57 4.62 624 233 236 3 859.11 2.66 625 234 237 3 878.12 2.50 626 234 240 6 117.66 27.58 627 234 243 9 129.22 39.51 628 235 240 5 252.38 22.07 629 237 240 3 856.20 3.18 630 238 241 3 870.41 0.61 631 239 242 3 864.18 0.45 632 239 243 4 856.25 4.86 633 240 243 3 856.24 4.86 634 240 244 4 844.79 0.76 635 241 244 3 839.93 1.67 636 241 245 4 839.92 1.68 637 242 245 3 855.89 5.03 638 242 246 4 833.50 1.54 639 243 246 3 833.06 1.32 640 243 247 4 857.91 2.85 641 244 247 3 865.32 2.44 642 244 248 4 857.91 2.85 643 245 248 3 871.00 0.41