BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 204 201 138.69 14.34 2 3 205 202 138.56 14.31 3 3 224 221 110.14 0.04 4 3 227 224 109.97 0.01 5 4 34 30 664.78 0.26 6 4 35 31 664.78 0.25 7 4 36 32 664.73 0.26 8 4 37 33 664.70 0.26 9 4 38 34 664.70 0.26 10 4 205 201 138.77 14.34 11 5 37 32 664.73 0.26 12 5 38 33 664.72 0.27 13 5 221 216 110.03 0.01 14 5 227 222 110.02 0.00 15 5 247 242 17.65 0.88 16 6 38 32 664.73 0.26 17 6 40 34 664.72 0.26 18 6 90 84 348.74 0.63 19 6 124 118 304.99 2.81 20 6 161 155 289.01 10.79 21 6 207 201 138.69 14.29 22 6 227 221 110.27 0.06 23 6 228 222 109.98 0.01 24 6 229 223 109.98 0.01 25 7 37 30 664.80 0.26 26 7 38 31 664.76 0.26 27 7 39 32 664.72 0.26 28 7 40 33 664.72 0.26 29 7 229 222 110.00 0.01 30 7 240 233 50.06 0.04 31 7 244 237 34.77 1.08 32 7 247 240 20.26 0.43 33 8 40 32 664.72 0.26 34 8 42 34 664.70 0.26 35 8 229 221 110.19 0.00 36 8 230 222 109.98 0.01 37 8 231 223 109.98 0.01 38 9 40 31 664.76 0.26 39 9 41 32 664.70 0.26 40 9 42 33 664.70 0.26 41 9 231 222 109.98 0.01 42 9 235 226 80.42 0.09 43 9 240 231 50.22 0.04 44 10 42 32 664.73 0.25 45 10 64 54 449.47 0.56 46 10 92 82 353.10 2.33 47 10 95 85 352.81 2.49 48 10 230 220 110.69 0.03 49 10 231 221 110.14 0.03 50 10 232 222 109.98 0.01 51 10 235 225 85.17 0.31 52 11 14 3 867.37 3.00 53 11 20 9 666.68 0.70 54 11 23 12 666.15 0.45 55 11 24 13 666.16 0.45 56 11 27 16 665.89 0.34 57 11 41 30 664.80 0.25 58 11 42 31 664.77 0.24 59 11 43 32 664.73 0.25 60 11 51 40 563.35 7.53 61 11 64 53 449.69 0.46 62 11 235 224 102.52 1.33 63 11 236 225 82.38 0.41 64 12 44 32 664.75 0.26 65 12 64 52 449.64 0.48 66 12 95 83 353.17 2.33 67 12 97 85 353.48 2.20 68 12 232 220 110.66 0.03 69 13 44 31 664.77 0.25 70 14 44 30 664.77 0.25 71 14 236 222 109.86 0.07 72 15 19 4 840.66 3.65 73 15 20 5 831.61 2.71 74 15 47 32 664.35 0.22 75 16 19 3 843.97 2.05 76 16 20 4 841.37 3.30 77 16 47 31 664.34 0.23 78 17 20 3 854.62 3.27 79 17 21 4 843.90 2.04 80 17 50 33 655.33 2.23 81 18 21 3 847.53 0.23 82 18 22 4 849.09 4.66 83 19 22 3 849.10 4.66 84 19 23 4 849.10 4.66 85 20 23 3 849.09 4.67 86 20 24 4 849.08 4.67 87 20 47 27 664.30 0.21 88 20 50 30 598.23 2.62 89 20 51 31 572.36 3.97 90 20 54 34 501.01 22.67 91 21 24 3 856.64 0.89 92 21 25 4 856.65 0.88 93 21 54 33 501.00 22.68 94 22 25 3 874.53 3.43 95 22 26 4 856.12 0.60 96 23 26 3 874.53 3.43 97 23 27 4 856.13 0.59 98 23 235 212 109.50 0.18 99 23 236 213 96.48 0.83 100 23 237 214 81.81 0.70 101 23 238 215 80.37 0.07 102 24 27 3 861.90 3.47 103 24 28 4 856.10 0.57 104 24 51 27 562.06 7.87 105 24 54 30 455.39 0.03 106 24 55 31 455.30 0.03 107 25 28 3 873.38 2.27 108 25 29 4 850.66 2.16 109 26 29 3 873.38 2.27 110 26 238 212 75.57 2.06 111 26 241 215 58.17 0.01 112 27 30 3 865.68 5.37 113 27 55 28 455.31 0.02 114 27 59 32 451.98 0.41 115 27 235 208 96.53 0.81 116 27 237 210 80.46 0.07 117 27 240 213 52.21 1.03 118 27 241 214 49.16 0.32 119 28 54 26 455.36 0.04 120 28 55 27 454.17 0.54 121 28 56 28 454.06 0.51 122 28 58 30 453.73 0.67 123 28 59 31 452.12 0.34 124 30 58 28 451.44 0.40 125 30 241 211 49.80 0.06 126 30 244 214 47.41 0.56 127 30 245 215 47.34 0.55 128 30 248 218 41.37 1.27 129 32 39 7 665.25 0.27 130 32 58 26 450.19 0.57 131 32 59 27 450.20 0.57 132 32 248 216 28.18 1.56 133 33 36 3 871.87 6.90 134 33 37 4 830.71 2.81 135 34 37 3 849.69 2.65 136 34 58 24 449.83 0.49 137 37 244 207 38.62 0.18 138 37 247 210 21.04 0.04 139 37 248 211 17.45 0.94 140 38 205 167 141.28 15.21 141 39 59 20 449.79 0.47 142 39 60 21 449.79 0.47 143 39 244 205 37.51 0.72 144 40 60 20 449.79 0.47 145 40 61 21 449.75 0.46 146 40 62 22 449.74 0.46 147 40 90 50 353.32 2.26 148 41 55 14 452.46 0.15 149 41 59 18 449.97 0.48 150 41 60 19 449.90 0.48 151 41 61 20 449.75 0.46 152 41 62 21 449.74 0.46 153 42 62 20 449.74 0.46 154 42 63 21 449.72 0.47 155 42 64 22 449.64 0.47 156 42 90 48 353.47 2.23 157 42 91 49 353.44 2.23 158 42 92 50 353.40 2.24 159 42 124 82 353.30 2.19 160 43 47 4 763.00 39.87 161 43 48 5 672.41 60.22 162 43 51 8 562.24 8.06 163 43 62 19 449.90 0.48 164 43 64 21 449.75 0.48 165 44 47 3 853.00 2.04 166 44 48 4 835.95 1.14 167 44 64 20 449.77 0.48 168 45 48 3 848.23 5.00 169 47 50 3 853.45 2.40 170 47 51 4 836.31 2.08 171 48 51 3 856.62 0.95 172 48 52 4 843.24 1.39 173 48 61 13 450.31 0.56 174 48 62 14 450.30 0.55 175 48 63 15 450.09 0.48 176 48 88 40 353.72 2.22 177 49 52 3 856.43 1.04 178 49 53 4 850.18 2.08 179 50 53 3 863.11 1.81 180 50 54 4 855.36 0.10 181 51 54 3 856.93 0.68 182 51 55 4 854.73 0.21 183 52 55 3 859.85 2.14 184 52 56 4 859.85 2.14 185 52 57 5 843.02 1.29 186 52 59 7 795.26 0.07 187 52 60 8 561.98 53.47 188 52 61 9 450.82 0.78 189 55 59 4 829.53 8.21 190 55 61 6 668.98 0.04 191 56 59 3 879.33 5.86 192 59 244 185 41.91 0.24 193 60 89 29 353.47 2.23 194 60 90 30 353.44 2.23 195 61 89 28 353.48 2.23 196 61 90 29 353.44 2.23 197 62 88 26 353.56 2.22 198 62 90 28 353.44 2.23 199 63 78 15 355.30 2.08 200 63 83 20 354.47 2.12 201 63 88 25 354.06 2.14 202 63 90 27 353.50 2.23 203 63 91 28 353.47 2.22 204 63 92 29 353.45 2.23 205 64 92 28 353.44 2.23 206 65 92 27 353.53 2.23 207 65 98 33 353.54 2.20 208 65 112 47 354.28 2.17 209 66 78 12 355.31 2.08 210 66 79 13 355.27 2.07 211 66 80 14 355.18 2.08 212 66 91 25 353.99 2.18 213 66 93 27 353.62 2.23 214 66 112 46 354.29 2.17 215 66 116 50 354.37 2.18 216 67 77 10 356.24 2.09 217 67 78 11 356.17 2.07 218 67 79 12 355.29 2.07 219 67 112 45 354.32 2.16 220 68 108 40 354.30 2.17 221 68 111 43 354.34 2.17 222 68 112 44 354.32 2.16 223 69 74 5 629.65 26.22 224 69 79 10 356.86 2.38 225 70 74 4 895.63 3.43 226 70 79 9 356.87 2.39 227 70 81 11 356.88 2.38 228 71 74 3 963.61 2.98 229 71 79 8 356.92 2.37 230 71 81 10 356.92 2.39 231 71 82 11 356.92 2.39 232 74 77 3 880.76 2.50 233 78 82 4 833.87 3.89 234 79 82 3 851.56 5.33 235 79 83 4 847.71 0.74 236 80 83 3 854.71 2.75 237 80 84 4 854.71 2.75 238 80 115 35 361.31 3.90 239 80 116 36 358.94 2.92 240 80 119 39 407.14 24.91 241 80 121 41 407.08 24.89 242 81 84 3 854.72 2.75 243 81 85 4 847.71 0.74 244 81 119 38 458.34 0.36 245 82 85 3 851.86 1.32 246 82 86 4 847.71 0.75 247 83 86 3 855.90 0.20 248 83 87 4 852.74 1.78 249 83 88 5 634.76 88.54 250 83 121 38 464.09 2.21 251 84 87 3 853.52 1.39 252 84 119 35 464.22 2.16 253 84 120 36 473.83 2.66 254 84 121 37 464.09 2.21 255 88 121 33 535.86 1.26 256 89 121 32 535.86 1.26 257 89 122 33 535.84 1.26 258 90 122 32 535.85 1.26 259 90 124 34 535.84 1.26 260 90 161 71 535.85 1.26 261 91 116 25 536.28 1.24 262 91 121 30 536.16 1.21 263 91 122 31 536.10 1.18 264 91 123 32 535.90 1.24 265 91 124 33 535.89 1.24 266 92 95 3 918.10 0.35 267 92 124 32 535.90 1.24 268 92 126 34 535.85 1.25 269 92 230 138 111.81 0.38 270 93 109 16 536.17 1.19 271 93 112 19 536.33 1.22 272 93 113 20 536.29 1.23 273 93 116 23 536.34 1.23 274 93 123 30 536.16 1.20 275 93 124 31 536.00 1.22 276 93 125 32 535.90 1.24 277 93 126 33 535.86 1.26 278 94 99 5 671.37 56.98 279 94 109 15 536.17 1.18 280 94 126 32 535.87 1.25 281 95 98 3 844.25 2.42 282 95 99 4 830.38 1.73 283 95 126 31 535.94 1.23 284 95 165 70 535.86 1.27 285 95 230 135 114.29 0.82 286 96 99 3 830.38 1.73 287 96 100 4 805.82 8.73 288 96 165 69 535.85 1.27 289 96 167 71 534.11 1.27 290 96 170 74 493.75 1.97 291 97 100 3 842.64 7.86 292 97 101 4 807.62 9.63 293 98 102 4 798.10 6.38 294 98 103 5 667.34 59.00 295 98 104 6 537.15 1.42 296 99 103 4 537.57 1.61 297 99 104 5 537.15 1.42 298 99 106 7 537.56 1.61 299 99 109 10 536.66 1.17 300 99 126 27 536.28 1.24 301 99 127 28 536.25 1.23 302 99 146 47 536.47 1.17 303 104 108 4 817.11 3.02 304 104 112 8 797.56 6.77 305 105 108 3 847.87 1.52 306 105 109 4 846.19 3.08 307 106 109 3 851.48 0.43 308 106 110 4 846.19 3.08 309 106 145 39 536.73 1.11 310 106 146 40 536.62 1.09 311 106 149 43 536.75 1.11 312 107 110 3 852.48 0.07 313 107 111 4 842.50 1.24 314 108 111 3 842.50 1.24 315 108 112 4 835.17 2.43 316 109 112 3 845.99 2.98 317 109 113 4 842.24 1.36 318 109 125 16 536.71 1.12 319 109 150 41 536.97 1.12 320 110 113 3 845.74 3.10 321 110 114 4 842.26 1.37 322 110 149 39 536.94 1.14 323 110 150 40 536.93 1.14 324 110 152 42 537.52 0.88 325 110 153 43 537.56 0.90 326 111 114 3 845.83 3.06 327 111 115 4 842.32 1.35 328 112 115 3 849.61 2.29 329 112 116 4 849.61 2.29 330 113 116 3 849.61 2.29 331 113 117 4 849.61 2.29 332 113 123 10 537.12 1.10 333 113 125 12 536.98 1.17 334 113 153 40 547.19 3.46 335 113 160 47 537.14 1.09 336 114 117 3 866.58 5.10 337 114 118 4 842.27 5.96 338 114 153 39 564.62 5.25 339 115 118 3 853.19 0.49 340 115 119 4 853.19 0.49 341 116 119 3 857.82 0.83 342 116 120 4 840.47 5.86 343 116 121 5 690.58 0.82 344 117 120 3 845.75 3.23 345 117 121 4 816.15 2.60 346 117 153 36 701.31 6.20 347 117 154 37 701.33 6.19 348 117 155 38 701.44 6.13 349 117 160 43 701.30 6.16 350 122 159 37 780.64 0.80 351 123 159 36 780.74 0.79 352 123 160 37 780.69 0.80 353 123 161 38 780.69 0.80 354 124 161 37 780.69 0.80 355 124 163 39 780.67 0.81 356 124 230 106 111.68 0.47 357 125 150 25 780.98 0.82 358 125 160 35 780.81 0.80 359 125 161 36 780.76 0.81 360 125 162 37 780.69 0.81 361 125 163 38 780.69 0.81 362 126 163 37 780.70 0.80 363 126 165 39 780.65 0.81 364 126 230 104 114.34 0.73 365 127 143 16 781.35 0.82 366 127 146 19 781.32 0.82 367 127 147 20 781.32 0.82 368 127 150 23 781.33 0.82 369 127 162 35 780.86 0.79 370 127 163 36 780.75 0.79 371 127 164 37 780.70 0.80 372 127 165 38 780.69 0.81 373 127 188 61 202.62 1.00 374 128 143 15 781.37 0.82 375 128 146 18 781.36 0.83 376 128 164 36 780.73 0.80 377 128 165 37 780.70 0.81 378 128 167 39 780.65 0.82 379 129 133 4 835.18 5.04 380 129 134 5 827.40 1.41 381 129 139 10 781.45 0.84 382 129 142 13 781.43 0.82 383 129 143 14 781.44 0.83 384 129 164 35 780.86 0.79 385 129 167 38 780.66 0.82 386 129 168 39 780.64 0.81 387 130 133 3 865.99 5.51 388 130 134 4 836.33 4.53 389 130 167 37 780.66 0.82 390 130 168 38 780.63 0.82 391 131 134 3 854.23 1.10 392 131 135 4 836.33 4.53 393 131 168 37 780.63 0.82 394 131 172 41 724.04 3.33 395 131 174 43 706.70 2.84 396 132 135 3 864.56 6.26 397 132 136 4 836.34 4.54 398 133 136 3 856.31 2.14 399 133 137 4 835.26 5.08 400 133 138 5 803.95 9.64 401 133 142 9 781.60 0.83 402 134 137 3 828.52 8.45 403 134 139 5 781.97 0.63 404 134 168 34 780.74 0.80 405 134 180 46 230.06 1.20 406 138 141 3 846.31 7.12 407 138 142 4 826.61 0.70 408 139 142 3 855.08 2.73 409 139 143 4 827.86 0.61 410 140 143 3 848.19 5.43 411 140 144 4 829.35 0.13 412 140 187 47 202.82 0.98 413 141 144 3 862.32 1.64 414 141 145 4 829.53 0.04 415 142 145 3 864.01 0.79 416 142 146 4 829.71 0.03 417 143 146 3 854.23 0.89 418 143 147 4 845.67 5.17 419 143 164 21 780.85 0.79 420 143 188 45 202.66 0.98 421 143 191 48 202.61 0.98 422 144 147 3 852.16 5.37 423 144 148 4 851.79 5.55 424 144 191 47 202.50 1.03 425 145 148 3 854.03 4.46 426 145 149 4 854.03 4.46 427 146 149 3 862.90 0.03 428 146 150 4 862.89 0.03 429 147 150 3 865.39 1.49 430 147 151 4 854.01 4.45 431 147 191 44 202.43 1.04 432 148 151 3 862.66 0.13 433 148 195 47 202.19 1.12 434 150 153 3 868.87 0.18 435 150 162 12 780.82 0.79 436 151 154 3 864.05 0.78 437 151 195 44 201.12 0.82 438 151 196 45 199.99 1.35 439 154 157 3 873.88 2.48 440 154 160 6 781.05 0.85 441 154 204 50 191.46 2.56 442 156 159 3 875.28 1.07 443 156 160 4 838.82 0.78 444 157 192 35 201.47 0.96 445 157 196 39 199.18 1.02 446 157 200 43 191.63 2.59 447 157 203 46 191.56 2.60 448 157 204 47 191.44 2.56 449 158 161 3 922.96 1.55 450 158 203 45 191.44 2.57 451 158 205 47 191.42 2.58 452 160 204 44 191.34 2.54 453 161 205 44 189.99 2.35 454 161 207 46 184.10 4.16 455 162 188 26 202.12 0.96 456 162 192 30 201.18 0.76 457 162 204 42 191.34 2.55 458 162 205 43 191.19 2.53 459 162 206 44 184.10 4.15 460 162 207 45 184.10 4.16 461 163 207 44 181.53 5.43 462 163 208 45 181.52 5.43 463 163 209 46 179.27 4.98 464 163 230 67 114.25 0.86 465 164 184 20 202.60 0.98 466 164 185 21 202.59 0.96 467 164 188 24 202.56 0.96 468 164 207 43 185.11 3.66 469 164 209 45 179.33 4.96 470 165 170 5 836.10 0.72 471 165 209 44 179.19 5.01 472 166 169 3 874.80 5.80 473 166 170 4 848.06 1.06 474 166 208 42 181.59 5.42 475 166 209 43 181.47 5.41 476 166 210 44 179.12 5.07 477 167 170 3 843.86 3.16 478 168 172 4 830.38 3.58 479 168 174 6 747.08 31.26 480 168 175 7 542.42 70.62 481 168 176 8 375.68 6.11 482 168 180 12 230.10 1.21 483 169 172 3 865.13 2.36 484 169 174 5 391.62 0.45 485 169 175 6 377.10 6.82 486 169 176 7 240.38 4.09 487 176 180 4 821.62 0.62 488 176 208 32 189.50 2.84 489 177 180 3 849.38 0.26 490 177 181 4 859.72 2.55 491 178 181 3 859.72 2.55 492 178 182 4 859.73 2.55 493 178 219 41 131.10 2.61 494 178 220 42 130.91 2.52 495 178 223 45 126.14 3.06 496 179 182 3 862.40 3.48 497 179 183 4 860.13 2.35 498 180 183 3 862.65 3.55 499 180 184 4 860.19 2.32 500 181 184 3 862.78 1.43 501 181 185 4 857.74 1.09 502 181 208 27 190.85 2.52 503 181 220 39 130.36 2.25 504 181 226 45 119.99 1.29 505 182 185 3 858.61 3.52 506 182 186 4 845.38 3.08 507 182 223 41 120.30 1.34 508 182 224 42 120.37 1.31 509 182 225 43 120.17 1.36 510 182 226 44 120.00 1.29 511 183 186 3 853.44 0.93 512 183 187 4 845.37 3.10 513 184 187 3 855.91 2.12 514 184 188 4 843.59 1.55 515 185 188 3 853.14 0.72 516 185 189 4 855.39 2.23 517 185 206 21 191.33 2.55 518 185 207 22 191.33 2.55 519 185 208 23 191.28 2.56 520 185 225 40 119.98 1.30 521 185 226 41 119.72 1.17 522 186 189 3 857.12 1.36 523 186 190 4 857.12 1.36 524 186 225 39 120.00 1.29 525 187 190 3 862.77 1.46 526 187 191 4 857.12 1.36 527 188 191 3 855.39 2.23 528 188 192 4 855.39 2.23 529 188 206 18 191.39 2.58 530 189 192 3 855.56 2.20 531 189 193 4 837.31 1.95 532 189 201 12 191.80 2.64 533 189 202 13 191.79 2.64 534 189 204 15 191.68 2.60 535 189 206 17 191.41 2.58 536 189 225 36 119.89 1.27 537 189 227 38 119.73 1.20 538 190 193 3 840.87 1.12 539 190 194 4 837.31 1.95 540 191 194 3 851.96 4.42 541 191 195 4 837.13 1.85 542 192 195 3 865.70 2.44 543 192 196 4 821.50 2.05 544 192 197 5 815.40 0.95 545 192 201 9 195.62 4.55 546 192 204 12 191.73 2.61 547 193 196 3 856.12 2.35 548 193 197 4 758.21 29.54 549 193 198 5 522.53 134.75 550 193 201 8 198.02 1.99 551 196 200 4 835.34 7.82 552 197 200 3 852.96 0.53 553 197 201 4 851.69 0.10 554 198 201 3 857.65 1.81 555 198 202 4 851.71 0.12 556 199 202 3 854.96 0.46 557 199 203 4 851.71 0.12 558 200 203 3 864.89 2.61 559 200 204 4 851.01 0.47 560 201 204 3 864.89 2.61 561 204 227 23 116.89 0.97 562 205 227 22 116.23 0.67 563 206 226 20 116.92 0.99 564 206 227 21 116.34 0.70 565 207 227 20 116.23 0.67 566 207 228 21 116.01 0.62 567 207 229 22 115.90 0.61 568 208 212 4 872.94 0.68 569 208 220 12 120.48 0.54 570 208 226 18 117.05 1.03 571 208 228 20 116.12 0.62 572 208 229 21 115.92 0.61 573 208 230 22 115.92 0.61 574 209 230 21 115.83 0.58 575 211 216 5 845.29 3.73 576 212 216 4 845.29 3.73 577 212 217 5 554.60 6.29 578 212 220 8 320.99 26.88 579 212 229 17 116.08 0.65 580 212 231 19 115.94 0.61 581 212 234 22 114.78 0.61 582 212 243 31 50.02 0.02 583 213 216 3 871.85 3.38 584 213 217 4 819.97 8.93 585 213 234 21 115.74 0.58 586 213 243 30 50.04 0.03 587 214 217 3 863.38 0.89 588 214 239 25 50.50 0.04 589 214 242 28 50.06 0.04 590 214 243 29 49.93 0.03 591 216 219 3 864.09 5.74 592 216 220 4 849.58 1.52 593 217 220 3 861.62 4.19 594 217 221 4 849.58 1.52 595 217 229 12 119.57 1.25 596 217 243 26 46.66 0.23 597 217 246 29 34.41 0.03 598 218 221 3 877.80 3.90 599 218 222 4 845.35 0.60 600 218 246 28 33.48 0.44 601 219 222 3 877.80 3.90 602 219 223 4 847.43 0.17 603 220 223 3 872.52 3.10 604 220 226 6 240.16 1.65 605 220 229 9 119.97 1.28 606 221 226 5 229.22 3.82 607 221 227 6 123.84 2.93 608 221 228 7 120.06 1.32 609 221 229 8 120.06 1.32 610 221 246 25 32.45 0.94 611 221 247 26 22.30 0.10 612 223 226 3 822.36 4.43 613 229 243 14 44.84 0.24 614 231 234 3 876.49 1.66 615 231 235 4 804.30 2.35 616 231 240 9 52.48 1.15 617 231 243 12 49.69 0.05 618 232 235 3 870.29 4.75 619 233 236 3 868.77 2.17 620 234 237 3 878.12 2.50 621 234 240 6 134.89 36.20 622 234 243 9 85.59 17.58 623 235 240 5 218.49 0.04 624 237 240 3 864.48 0.96 625 238 241 3 867.65 1.28 626 239 242 3 864.18 0.46 627 239 243 4 859.59 3.18 628 240 243 3 856.13 4.91 629 240 244 4 844.80 0.76 630 241 244 3 859.51 3.21 631 241 245 4 844.40 3.91 632 242 245 3 856.12 4.92 633 242 246 4 830.57 3.00 634 243 246 3 856.99 0.48 635 244 247 3 865.32 2.44 636 244 248 4 844.93 3.65 637 245 248 3 846.83 4.60