BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 6 4 838.33 0.82 2 3 6 3 844.81 4.05 3 3 7 4 840.86 6.49 4 4 7 3 861.07 0.70 5 4 8 4 840.86 6.48 6 4 223 219 58.28 0.83 7 5 8 3 861.07 0.70 8 5 9 4 840.86 6.48 9 5 208 203 58.12 0.92 10 5 223 218 58.12 0.92 11 5 225 220 58.11 0.92 12 5 236 231 54.45 2.06 13 6 9 3 845.19 8.64 14 6 10 4 833.70 3.39 15 6 236 230 54.45 2.06 16 6 239 233 47.71 0.55 17 6 240 234 43.48 0.48 18 6 243 237 34.71 0.35 19 7 10 3 859.62 0.01 20 7 11 4 830.95 4.76 21 8 11 3 865.17 1.81 22 8 12 4 818.95 1.26 23 8 180 172 146.95 42.01 24 8 206 198 67.49 2.64 25 8 223 215 59.13 0.83 26 9 12 3 840.13 0.29 27 9 15 6 650.13 2.75 28 9 86 77 503.50 30.01 29 9 91 82 507.12 28.21 30 9 208 199 59.91 0.55 31 9 236 227 57.77 1.02 32 9 240 231 53.97 0.62 33 10 244 234 32.27 0.49 34 12 15 3 861.64 0.39 35 12 91 79 622.55 1.98 36 12 179 167 313.20 0.70 37 12 180 168 275.52 0.56 38 13 93 80 622.66 1.99 39 13 179 166 316.59 0.29 40 14 93 79 622.64 1.98 41 14 174 160 316.84 0.28 42 14 177 163 316.73 0.28 43 14 178 164 316.67 0.27 44 14 179 165 316.63 0.28 45 15 86 71 622.79 1.94 46 15 91 76 622.79 1.95 47 15 92 77 622.72 1.97 48 15 93 78 622.71 1.97 49 15 180 165 316.60 0.28 50 15 182 167 316.60 0.28 51 16 92 76 622.78 1.94 52 16 93 77 622.75 1.96 53 16 94 78 622.65 1.99 54 16 174 158 316.97 0.32 55 16 175 159 316.88 0.29 56 16 178 162 316.76 0.29 57 16 180 164 316.70 0.27 58 16 181 165 316.62 0.27 59 16 182 166 316.62 0.27 60 17 83 66 622.79 1.94 61 17 92 75 622.82 1.94 62 17 94 77 622.75 1.96 63 17 95 78 622.64 1.98 64 17 96 79 622.64 1.98 65 17 181 164 316.69 0.27 66 17 182 165 316.64 0.28 67 18 96 78 622.65 1.98 68 18 98 80 622.64 1.98 69 18 115 97 556.56 0.40 70 18 181 163 316.75 0.29 71 18 182 164 316.70 0.28 72 18 183 165 316.61 0.28 73 18 184 166 316.61 0.28 74 18 186 168 313.23 0.71 75 18 193 175 211.97 1.45 76 18 196 178 211.33 1.61 77 18 197 179 211.27 1.59 78 19 23 4 813.84 6.09 79 19 83 64 623.11 1.97 80 19 95 76 622.82 1.94 81 19 96 77 622.71 1.97 82 19 97 78 622.65 1.99 83 19 98 79 622.65 1.99 84 19 104 85 558.28 0.32 85 19 184 165 316.62 0.28 86 20 23 3 876.25 3.54 87 20 98 78 622.66 1.99 88 20 99 79 622.64 1.99 89 20 184 164 316.70 0.28 90 20 185 165 316.62 0.28 91 20 186 166 313.61 0.90 92 21 24 3 849.34 4.78 93 21 25 4 833.60 3.70 94 21 31 10 623.95 2.48 95 21 99 78 622.65 1.99 96 21 185 164 316.69 0.28 97 22 25 3 862.46 1.79 98 22 31 9 630.84 3.69 99 22 185 163 316.67 0.28 100 23 77 54 624.33 2.25 101 23 79 56 623.94 2.17 102 23 80 57 623.95 2.17 103 23 83 60 623.86 2.12 104 23 104 81 563.10 0.11 105 23 184 161 316.73 0.29 106 23 185 162 316.63 0.28 107 23 229 206 106.33 0.50 108 24 27 3 849.43 1.12 109 24 28 4 836.72 1.71 110 24 29 5 704.18 10.08 111 24 77 53 624.36 2.23 112 24 80 56 624.27 2.25 113 24 99 75 622.80 1.96 114 24 101 77 622.63 2.00 115 24 103 79 571.21 3.89 116 25 28 3 840.30 3.50 117 25 29 4 729.04 17.05 118 25 31 6 636.87 3.33 119 26 77 51 624.52 2.21 120 26 228 202 142.87 4.88 121 26 229 203 138.47 4.43 122 26 230 204 129.74 2.69 123 27 74 47 624.75 2.17 124 27 75 48 624.60 2.23 125 27 76 49 624.57 2.22 126 27 77 50 624.53 2.21 127 27 80 53 624.54 2.22 128 27 103 76 619.81 0.90 129 28 70 42 625.04 2.21 130 29 70 41 625.04 2.21 131 29 71 42 625.04 2.21 132 29 74 45 624.77 2.18 133 29 103 74 622.56 2.03 134 30 36 6 635.24 2.44 135 30 37 7 634.73 2.19 136 30 70 40 625.11 2.21 137 31 34 3 845.50 3.06 138 31 36 5 707.34 8.71 139 31 37 6 635.25 2.43 140 32 44 12 630.73 3.00 141 32 45 13 631.38 3.32 142 32 48 16 630.91 3.08 143 33 43 10 633.10 2.47 144 33 187 154 316.75 0.29 145 33 189 156 316.70 0.27 146 33 190 157 316.64 0.26 147 34 37 3 866.81 3.64 148 34 38 4 871.46 1.31 149 35 38 3 886.48 2.21 150 37 44 7 638.33 0.61 151 38 41 3 854.34 4.61 152 38 42 4 752.51 18.95 153 38 43 5 677.04 6.30 154 39 42 3 846.43 8.56 155 39 47 8 683.95 2.91 156 39 59 20 632.72 1.48 157 39 62 23 632.46 1.57 158 39 63 24 628.03 2.96 159 39 66 27 628.07 2.97 160 42 46 4 831.12 3.15 161 42 47 5 807.97 8.31 162 42 59 17 634.42 1.35 163 43 46 3 865.58 2.57 164 43 47 4 827.76 1.57 165 44 47 3 865.58 2.57 166 44 48 4 827.76 1.57 167 44 63 19 633.44 0.86 168 45 49 4 827.75 1.57 169 47 52 5 712.21 7.08 170 47 59 12 634.70 1.24 171 47 60 13 633.88 0.89 172 47 63 16 633.52 0.85 173 48 63 15 633.94 0.86 174 48 64 16 633.65 0.78 175 48 158 110 367.79 8.96 176 49 52 3 819.00 3.10 177 49 53 4 818.73 3.27 178 49 158 109 367.87 8.92 179 50 53 3 841.56 0.20 180 50 54 4 831.82 0.94 181 50 158 108 368.85 8.85 182 50 159 109 368.82 8.86 183 50 161 111 367.80 8.96 184 51 54 3 845.04 1.62 185 51 55 4 837.31 1.81 186 52 55 3 844.61 1.84 187 52 56 4 839.12 4.58 188 52 60 8 756.54 3.08 189 53 56 3 874.09 3.08 190 53 57 4 806.81 7.53 191 53 60 7 766.62 1.99 192 53 61 8 779.27 5.70 193 53 125 72 546.06 3.26 194 53 151 98 371.11 8.57 195 53 152 99 371.04 8.59 196 53 153 100 370.75 8.62 197 53 158 105 369.28 8.88 198 54 57 3 874.09 3.08 199 56 59 3 861.98 6.52 200 56 60 4 833.19 1.65 201 57 60 3 849.00 1.74 202 57 61 4 822.69 3.95 203 57 142 85 426.07 2.55 204 58 61 3 858.37 5.21 205 58 62 4 826.26 0.89 206 58 139 81 428.58 2.89 207 58 142 84 426.65 2.27 208 59 62 3 852.98 5.07 209 59 63 4 837.66 1.51 210 60 63 3 848.91 4.12 211 60 64 4 837.66 1.52 212 60 158 98 369.23 8.87 213 61 64 3 853.65 8.52 214 61 65 4 834.10 3.29 215 61 68 7 816.44 3.54 216 61 122 61 557.16 0.45 217 61 137 76 429.04 2.85 218 61 139 78 428.12 2.90 219 61 142 81 425.85 2.44 220 61 143 82 423.29 3.69 221 61 152 91 370.75 8.54 222 61 153 92 370.50 8.61 223 61 154 93 370.41 8.59 224 62 65 3 848.17 3.45 225 62 66 4 847.88 3.59 226 62 139 77 428.13 2.90 227 63 66 3 848.18 3.45 228 63 67 4 845.50 4.77 229 64 67 3 851.71 1.68 230 64 68 4 837.10 1.22 231 64 119 55 557.21 0.45 232 64 154 90 369.22 8.89 233 64 156 92 369.20 8.88 234 64 158 94 369.01 8.79 235 65 68 3 866.85 1.32 236 65 69 4 825.69 7.66 237 65 137 72 428.75 2.73 238 65 139 74 428.12 2.91 239 66 69 3 852.95 2.30 240 66 70 4 836.61 2.25 241 67 70 3 852.76 2.21 242 67 71 4 829.02 5.80 243 67 100 33 623.06 1.97 244 67 119 52 557.13 0.43 245 68 71 3 839.21 1.20 246 68 72 4 820.53 1.56 247 68 119 51 557.12 0.44 248 68 137 69 428.43 2.91 249 69 73 4 829.08 1.49 250 69 136 67 428.38 2.90 251 70 73 3 842.69 0.78 252 70 74 4 821.26 1.95 253 71 74 3 821.50 1.83 254 71 101 30 622.87 1.94 255 71 102 31 622.87 1.95 256 71 119 48 557.05 0.47 257 72 119 47 557.12 0.43 258 72 120 48 556.92 0.41 259 72 133 61 429.16 2.77 260 72 134 62 428.94 2.71 261 72 135 63 428.42 2.92 262 72 136 64 428.41 2.92 263 74 103 29 622.15 2.06 264 74 106 32 586.22 0.06 265 75 103 28 615.60 1.20 266 75 106 31 571.30 3.85 267 76 80 4 804.11 16.05 268 76 103 27 574.93 5.61 269 76 106 30 562.60 0.28 270 76 107 31 558.50 0.42 271 77 80 3 843.33 0.87 272 77 81 4 843.32 0.87 273 78 81 3 843.32 0.87 274 78 82 4 830.71 5.44 275 78 230 152 100.17 3.68 276 78 231 153 100.17 3.68 277 78 234 156 99.97 3.62 278 79 82 3 843.36 0.85 279 79 83 4 843.35 0.84 280 79 229 150 101.23 3.04 281 79 230 151 100.05 3.63 282 79 233 154 99.73 3.50 283 80 83 3 851.60 1.58 284 80 84 4 843.35 0.84 285 80 103 23 561.25 1.09 286 80 104 24 558.54 0.39 287 80 107 27 557.84 0.54 288 81 84 3 849.41 3.84 289 81 85 4 834.75 3.48 290 81 107 26 557.83 0.54 291 82 85 3 834.75 3.48 292 82 86 4 834.76 3.48 293 82 233 151 98.34 2.81 294 82 234 152 97.16 2.23 295 82 237 155 92.94 2.51 296 83 86 3 858.16 5.33 297 83 87 4 846.05 9.13 298 83 92 9 641.63 4.36 299 83 95 12 622.90 1.95 300 83 104 21 558.34 0.29 301 83 182 99 316.74 0.29 302 83 184 101 316.63 0.27 303 83 233 150 98.34 2.81 304 84 87 3 867.16 1.43 305 84 88 4 836.08 2.82 306 84 107 23 557.79 0.56 307 84 108 24 557.62 0.57 308 84 109 25 557.40 0.50 309 85 88 3 856.00 7.02 310 85 89 4 836.09 2.83 311 85 237 152 79.89 1.07 312 85 241 156 72.90 0.18 313 86 89 3 838.39 1.57 314 86 91 5 745.90 0.66 315 86 92 6 653.10 4.63 316 86 182 96 316.67 0.27 317 86 237 151 78.53 1.73 318 86 240 154 72.88 0.19 319 87 90 3 853.28 5.88 320 87 92 5 688.18 10.38 321 87 109 22 557.40 0.50 322 87 110 23 557.39 0.51 323 87 111 24 557.22 0.47 324 89 241 152 62.46 2.27 325 89 244 155 58.61 2.26 326 90 93 3 907.00 1.56 327 91 240 149 62.47 2.27 328 91 244 153 41.20 0.44 329 92 95 3 918.34 0.56 330 93 111 18 556.94 0.49 331 94 111 17 556.93 0.49 332 94 113 19 556.80 0.46 333 94 174 80 316.86 0.28 334 95 104 9 558.25 0.32 335 95 109 14 557.28 0.43 336 95 111 16 557.13 0.43 337 95 112 17 556.82 0.47 338 95 113 18 556.67 0.40 339 96 113 17 556.64 0.39 340 96 115 19 556.55 0.39 341 96 171 75 317.14 0.35 342 96 174 78 317.12 0.36 343 96 175 79 317.12 0.35 344 96 196 100 211.28 1.59 345 97 104 7 558.31 0.30 346 97 112 15 557.18 0.43 347 97 113 16 557.07 0.42 348 97 114 17 556.64 0.39 349 97 115 18 556.56 0.40 350 98 115 17 556.62 0.38 351 98 116 18 556.50 0.42 352 98 186 88 316.24 0.29 353 98 193 95 212.00 1.44 354 99 114 15 557.18 0.45 355 100 116 16 557.09 0.45 356 100 117 17 556.61 0.40 357 100 118 18 555.08 0.66 358 100 119 19 543.68 0.12 359 100 156 56 360.37 7.61 360 102 105 3 877.43 2.16 361 102 106 4 844.13 4.24 362 103 106 3 844.12 4.24 363 103 107 4 845.20 3.70 364 104 107 3 859.21 5.54 365 104 108 4 849.76 1.41 366 104 109 5 787.96 6.53 367 104 112 8 684.14 6.71 368 105 108 3 848.05 0.55 369 105 109 4 801.43 0.21 370 105 112 7 670.41 2.27 371 105 114 9 662.25 3.71 372 106 120 14 540.90 0.52 373 109 112 3 904.85 4.67 374 113 167 54 317.17 0.35 375 113 170 57 317.13 0.36 376 113 171 58 317.13 0.36 377 113 174 61 317.10 0.35 378 114 117 3 856.42 4.08 379 114 167 53 317.28 0.33 380 115 163 48 317.80 0.41 381 115 164 49 317.75 0.40 382 115 167 52 317.30 0.34 383 115 171 56 317.21 0.34 384 115 193 78 211.99 1.44 385 115 196 81 211.96 1.44 386 116 156 40 359.57 7.62 387 116 157 41 359.37 7.53 388 116 163 47 317.85 0.40 389 116 164 48 317.82 0.40 390 116 193 77 212.05 1.45 391 117 155 38 361.59 8.11 392 117 156 39 359.58 7.62 393 117 157 40 359.57 7.62 394 117 164 47 317.75 0.38 395 118 121 3 836.40 0.41 396 118 155 37 366.37 9.03 397 118 156 38 361.55 8.15 398 119 156 37 366.51 8.98 399 120 132 12 429.20 2.81 400 120 133 13 429.16 2.80 401 120 134 14 429.11 2.81 402 121 130 9 429.67 2.90 403 121 131 10 429.47 2.87 404 121 132 11 429.19 2.81 405 121 154 33 369.18 8.89 406 121 155 34 368.42 9.19 407 122 130 8 429.67 2.90 408 122 131 9 429.56 2.91 409 122 133 11 429.32 2.80 410 122 152 30 370.44 8.59 411 122 153 31 369.48 8.97 412 122 154 32 369.19 8.89 413 122 155 33 369.15 8.89 414 123 129 6 429.78 2.93 415 123 130 7 429.67 2.90 416 123 152 29 370.56 8.59 417 123 153 30 370.32 8.59 418 123 155 32 369.19 8.89 419 124 127 3 867.43 2.66 420 124 129 5 429.78 2.93 421 124 145 21 372.41 8.05 422 124 148 24 372.11 8.22 423 124 150 26 371.06 8.55 424 124 151 27 370.93 8.54 425 124 152 28 370.57 8.59 426 125 150 25 371.56 8.30 427 125 151 26 371.04 8.56 428 125 153 28 370.57 8.59 429 126 148 22 372.12 8.23 430 126 150 24 371.51 8.28 431 127 145 18 409.79 10.38 432 127 147 20 372.15 8.23 433 127 148 21 372.12 8.23 434 129 145 16 372.43 8.04 435 129 147 18 372.04 8.22 436 131 152 21 370.78 8.54 437 138 141 3 877.77 0.38 438 143 152 9 370.89 8.58 439 144 150 6 390.20 17.23 440 144 152 8 371.68 8.25 441 145 148 3 866.48 3.76 442 145 149 4 390.22 17.25 443 145 150 5 394.12 15.29 444 145 152 7 371.80 8.19 445 153 156 3 1056.52 1.76 446 153 158 5 888.73 1.28 447 153 161 8 746.75 4.06 448 154 158 4 930.50 2.81 449 157 161 4 818.38 13.55 450 157 162 5 803.11 5.92 451 157 163 6 397.79 20.52 452 157 164 7 337.60 9.47 453 158 161 3 838.20 0.58 454 163 168 5 740.20 12.15 455 163 192 29 212.03 1.46 456 164 167 3 856.83 1.32 457 166 169 3 846.66 5.79 458 166 170 4 832.22 0.48 459 167 170 3 846.45 0.43 460 167 171 4 835.50 1.67 461 168 171 3 852.49 4.58 462 168 172 4 838.87 2.01 463 168 192 24 211.99 1.45 464 168 196 28 211.76 1.34 465 169 172 3 848.08 5.09 466 169 173 4 838.89 2.02 467 170 173 3 852.65 7.39 468 170 174 4 846.58 5.84 469 171 174 3 851.23 8.16 470 171 175 4 839.86 2.48 471 171 196 25 211.62 1.34 472 172 175 3 873.75 9.15 473 172 176 4 842.43 3.75 474 172 196 24 211.62 1.34 475 172 199 27 210.66 1.42 476 173 176 3 873.75 9.14 477 173 177 4 842.44 3.75 478 174 177 3 852.21 1.34 479 174 178 4 842.43 3.75 480 175 178 3 839.61 3.30 481 175 181 6 692.77 20.48 482 175 196 21 211.12 1.56 483 175 199 24 209.62 1.10 484 175 200 25 209.32 1.01 485 176 199 23 209.62 1.10 486 176 203 27 209.22 1.07 487 178 200 22 209.36 0.99 488 179 205 26 208.88 0.92 489 179 206 27 208.87 0.92 490 180 206 26 208.82 0.93 491 180 208 28 208.69 0.96 492 181 197 16 211.06 1.58 493 181 200 19 209.75 1.01 494 181 206 25 208.94 0.94 495 181 207 26 208.80 0.95 496 181 208 27 208.60 1.01 497 182 208 26 208.53 1.04 498 182 210 28 208.50 1.04 499 182 233 51 98.33 2.81 500 183 186 3 908.24 0.72 501 183 197 14 211.29 1.59 502 183 207 24 209.09 1.00 503 183 208 25 208.89 0.96 504 183 209 26 208.53 1.04 505 183 210 27 208.50 1.05 506 183 217 34 153.49 1.06 507 184 210 26 208.50 1.05 508 184 229 45 102.65 2.33 509 185 210 25 208.60 1.08 510 185 211 26 208.50 1.04 511 186 211 25 208.58 1.07 512 187 215 28 156.34 0.18 513 187 216 29 155.32 0.69 514 188 194 6 319.76 53.27 515 188 215 27 169.28 7.64 516 188 216 28 155.30 0.69 517 188 217 29 155.25 0.69 518 189 194 5 819.89 0.75 519 189 216 27 155.34 0.67 520 190 194 4 843.87 1.29 521 191 194 3 869.32 4.95 522 191 195 4 852.99 3.18 523 192 195 3 868.03 2.54 524 192 196 4 859.39 1.05 525 193 196 3 870.47 1.32 526 193 197 4 876.15 1.53 527 194 197 3 898.33 7.01 528 195 198 3 864.69 1.56 529 195 199 4 850.25 2.00 530 196 199 3 857.11 3.12 531 196 200 4 846.61 0.36 532 197 200 3 855.00 3.83 533 197 201 4 839.77 3.04 534 197 207 10 209.08 1.01 535 197 217 20 153.47 1.05 536 198 201 3 855.00 3.83 537 198 202 4 838.28 3.67 538 198 220 22 153.09 0.97 539 199 202 3 865.00 1.17 540 199 203 4 839.17 3.23 541 200 203 3 865.90 4.66 542 200 204 4 838.25 2.77 543 200 205 5 784.37 7.32 544 200 206 6 770.28 14.36 545 201 204 3 851.03 3.19 546 201 205 4 818.57 4.27 547 201 207 6 732.47 3.29 548 201 220 19 153.03 1.00 549 201 222 21 152.88 1.00 550 206 222 16 152.75 1.00 551 207 217 10 153.47 1.05 552 207 220 13 153.10 0.96 553 207 221 14 152.88 1.00 554 207 222 15 152.85 1.01 555 207 223 16 152.79 0.98 556 208 223 15 152.71 1.01 557 208 225 17 152.18 0.79 558 209 215 6 654.87 1.16 559 209 217 8 153.48 1.06 560 209 218 9 153.44 1.04 561 209 221 12 152.99 0.97 562 209 223 14 152.88 1.00 563 209 224 15 152.46 0.92 564 209 225 16 152.34 0.87 565 210 225 15 152.34 0.87 566 210 227 17 152.14 0.78 567 210 229 19 106.32 0.51 568 210 232 22 99.98 3.61 569 210 233 23 99.97 3.60 570 211 215 4 860.89 1.01 571 211 225 14 152.78 1.00 572 211 226 15 152.44 0.92 573 211 227 16 152.16 0.79 574 212 226 14 152.52 0.91 575 212 227 15 152.18 0.80 576 212 228 16 152.16 0.79 577 213 226 13 152.88 0.99 578 214 218 4 843.22 0.65 579 214 226 12 152.94 1.02 580 215 218 3 844.41 1.03 581 215 219 4 873.52 3.39 582 216 219 3 915.73 4.42 583 217 220 3 876.09 2.10 584 217 221 4 835.06 4.71 585 218 221 3 846.75 0.27 586 218 224 6 152.91 1.00 587 225 232 7 373.67 133.19 588 225 236 11 99.62 3.44 589 227 232 5 729.00 3.07 590 228 231 3 870.77 0.40 591 228 232 4 840.89 4.93 592 229 232 3 849.75 4.03 593 229 233 4 800.06 9.17 594 230 234 4 831.67 0.32 595 231 234 3 842.61 6.92 596 231 235 4 828.99 1.65 597 232 235 3 856.82 0.58 598 232 236 4 830.23 0.81 599 233 236 3 848.38 3.31 600 233 237 4 830.23 0.81 601 234 237 3 848.38 3.31 602 234 238 4 838.61 5.94 603 235 238 3 846.16 4.43 604 235 239 4 838.63 5.96 605 236 239 3 854.62 2.02 606 236 240 4 838.51 6.04 607 237 240 3 851.95 0.68 608 237 241 4 851.94 0.68 609 238 241 3 851.95 0.68 610 238 242 4 851.94 0.68 611 239 242 3 851.95 0.68 612 239 243 4 838.41 6.08 613 240 243 3 834.31 0.53 614 240 244 4 817.22 4.51 615 241 244 3 838.44 6.09 616 242 245 3 865.38 0.36 617 243 246 3 885.97 3.65