BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 232 231 31.62 1.14 2 1 235 234 27.85 0.12 3 1 236 235 27.39 0.11 4 2 5 3 859.10 3.35 5 2 6 4 838.59 0.70 6 2 223 221 62.04 2.28 7 3 6 3 865.23 5.70 8 3 7 4 840.63 6.59 9 4 7 3 845.51 4.16 10 4 8 4 840.65 6.58 11 4 223 219 63.95 1.67 12 5 8 3 840.67 6.59 13 5 9 4 840.66 6.59 14 5 208 203 63.97 1.67 15 5 223 218 63.82 1.74 16 5 225 220 63.28 1.99 17 5 236 231 55.13 0.81 18 6 9 3 840.67 6.59 19 6 10 4 833.68 3.10 20 6 236 230 58.02 2.31 21 6 239 233 44.44 0.87 22 7 10 3 840.43 6.47 23 7 11 4 825.66 0.91 24 8 12 4 800.74 7.85 25 8 180 172 65.61 1.33 26 8 206 198 65.59 1.32 27 8 208 200 64.31 1.73 28 8 223 215 64.01 1.74 29 9 12 3 834.13 3.79 30 9 15 6 683.32 5.23 31 9 91 82 378.95 20.92 32 9 208 199 64.52 1.62 33 9 236 227 62.85 2.12 34 9 240 231 45.41 1.24 35 10 240 230 44.59 0.87 36 10 243 233 39.64 0.07 37 10 244 234 37.99 0.78 38 11 247 236 32.11 0.71 39 12 15 3 861.80 0.56 40 12 91 79 611.07 0.84 41 12 179 167 312.41 1.05 42 12 180 168 277.72 1.41 43 13 93 80 611.10 0.84 44 13 179 166 312.94 1.31 45 14 93 79 611.05 0.82 46 14 174 160 313.46 1.33 47 14 177 163 313.30 1.33 48 14 178 164 313.24 1.35 49 14 179 165 313.16 1.33 50 15 86 71 611.31 0.82 51 15 91 76 611.26 0.82 52 15 92 77 611.19 0.80 53 15 93 78 611.13 0.83 54 15 179 164 313.26 1.34 55 15 180 165 313.05 1.28 56 15 182 167 312.97 1.31 57 16 92 76 611.22 0.81 58 16 94 78 611.11 0.81 59 16 174 158 313.59 1.32 60 16 175 159 313.51 1.34 61 16 178 162 313.36 1.34 62 16 180 164 313.30 1.34 63 16 181 165 313.07 1.28 64 16 182 166 313.06 1.29 65 17 83 66 611.34 0.83 66 17 92 75 611.28 0.82 67 17 94 77 611.23 0.82 68 17 95 78 611.09 0.83 69 17 96 79 611.07 0.83 70 17 181 164 313.26 1.34 71 17 182 165 313.16 1.32 72 18 96 78 611.09 0.83 73 18 98 80 611.06 0.83 74 18 115 97 561.07 1.49 75 18 181 163 313.33 1.34 76 18 182 164 313.28 1.34 77 18 183 165 313.03 1.28 78 18 184 166 312.97 1.31 79 18 193 175 213.62 1.03 80 18 196 178 213.21 1.22 81 18 197 179 213.11 1.24 82 19 23 4 825.38 0.19 83 19 83 64 611.48 0.85 84 19 95 76 611.27 0.83 85 19 96 77 611.20 0.81 86 19 97 78 611.05 0.82 87 19 98 79 611.05 0.82 88 19 104 85 562.54 1.31 89 19 184 165 313.08 1.36 90 20 23 3 876.50 3.42 91 20 98 78 611.09 0.83 92 20 99 79 611.04 0.82 93 20 184 164 313.29 1.35 94 20 185 165 312.94 1.31 95 20 186 166 310.82 1.91 96 21 24 3 859.75 2.50 97 21 25 4 832.72 3.71 98 21 31 10 612.51 1.41 99 21 32 11 612.51 1.41 100 21 99 78 611.05 0.82 101 21 185 164 313.28 1.35 102 22 25 3 833.11 3.95 103 22 31 9 612.90 1.46 104 22 185 163 313.27 1.34 105 23 77 54 611.93 0.87 106 23 79 56 611.79 0.91 107 23 80 57 611.79 0.91 108 23 83 60 611.79 0.91 109 23 104 81 585.54 3.04 110 23 184 161 313.30 1.35 111 23 185 162 313.22 1.34 112 23 229 206 100.79 2.08 113 24 27 3 845.44 3.88 114 24 29 5 638.68 3.08 115 24 77 53 611.93 0.87 116 24 99 75 611.18 0.80 117 24 101 77 611.00 0.80 118 24 103 79 599.28 0.32 119 25 28 3 859.17 0.60 120 25 29 4 676.54 24.14 121 25 31 6 618.01 3.30 122 26 77 51 612.04 0.82 123 26 228 202 142.47 1.50 124 26 229 203 134.33 2.50 125 26 230 204 119.23 2.46 126 27 74 47 612.21 0.80 127 27 75 48 612.14 0.78 128 27 76 49 612.09 0.80 129 27 77 50 612.06 0.81 130 27 103 76 609.94 1.31 131 29 67 38 612.80 0.84 132 29 70 41 612.71 0.83 133 29 71 42 612.73 0.82 134 29 74 45 612.35 0.77 135 29 103 74 610.98 0.83 136 30 36 6 639.36 3.31 137 30 38 8 639.37 3.27 138 30 67 37 613.00 0.91 139 31 37 6 639.39 3.29 140 31 38 7 639.40 3.28 141 32 37 5 639.42 3.28 142 32 38 6 639.40 3.28 143 32 39 7 639.40 3.27 144 32 40 8 639.48 3.26 145 33 37 4 639.48 3.26 146 33 39 6 639.48 3.26 147 33 40 7 639.49 3.26 148 33 159 126 403.78 2.27 149 33 187 154 313.35 1.36 150 33 189 156 313.31 1.36 151 33 190 157 313.19 1.33 152 34 37 3 812.45 4.94 153 34 40 6 666.52 8.03 154 37 40 3 844.48 8.69 155 37 41 4 809.20 4.78 156 38 41 3 862.96 0.55 157 38 42 4 807.77 4.95 158 38 63 25 618.34 0.01 159 38 67 29 613.11 0.90 160 39 42 3 831.19 0.94 161 39 44 5 685.62 4.50 162 39 48 9 683.46 3.39 163 39 63 24 618.44 0.04 164 39 159 120 403.81 2.26 165 42 47 5 815.18 8.12 166 42 63 21 618.41 0.01 167 42 66 24 618.41 0.01 168 43 46 3 855.44 2.50 169 43 47 4 833.11 0.85 170 44 47 3 833.13 0.84 171 44 48 4 779.50 5.63 172 44 49 5 811.08 10.16 173 44 159 115 404.04 2.28 174 45 49 4 833.11 0.85 175 45 52 7 738.67 7.25 176 46 49 3 904.43 5.48 177 46 52 6 712.63 5.75 178 46 59 13 623.53 1.67 179 46 60 14 623.42 1.73 180 47 52 5 759.48 5.28 181 47 59 12 623.39 1.71 182 47 60 13 619.85 0.05 183 47 63 16 618.73 0.07 184 47 64 17 618.54 0.03 185 48 158 110 404.09 2.29 186 48 159 111 404.05 2.28 187 49 52 3 851.78 3.58 188 49 53 4 828.45 1.17 189 49 158 109 404.11 2.29 190 49 159 110 404.10 2.29 191 50 53 3 844.57 5.19 192 50 54 4 829.37 1.62 193 50 158 108 404.38 2.33 194 50 159 109 404.34 2.33 195 50 161 111 404.08 2.29 196 51 54 3 859.43 0.95 197 51 55 4 836.77 2.08 198 52 55 3 845.08 6.23 199 52 56 4 829.72 2.01 200 52 60 8 763.60 3.36 201 53 56 3 874.34 2.96 202 53 57 4 797.16 3.02 203 53 60 7 763.62 3.37 204 53 61 8 768.83 0.78 205 53 125 72 558.83 2.21 206 53 152 99 409.69 3.79 207 53 153 100 408.52 3.62 208 53 158 105 404.47 2.31 209 54 57 3 876.17 2.04 210 56 59 3 855.57 0.74 211 56 60 4 833.19 0.77 212 57 60 3 849.25 1.86 213 57 61 4 828.06 1.79 214 57 142 85 476.45 2.73 215 57 151 94 409.92 3.73 216 58 61 3 837.47 1.29 217 58 62 4 828.08 1.79 218 58 139 81 476.72 2.62 219 58 140 82 476.68 2.65 220 58 142 84 476.37 2.76 221 59 62 3 846.58 5.28 222 59 63 4 833.17 1.64 223 60 63 3 849.16 3.99 224 60 64 4 836.77 0.16 225 60 158 98 404.45 2.32 226 61 64 3 846.18 4.44 227 61 65 4 839.18 1.36 228 61 68 7 826.31 4.17 229 61 122 61 561.57 1.30 230 61 137 76 477.12 2.54 231 61 139 78 476.68 2.65 232 61 142 81 475.31 2.37 233 61 143 82 462.01 1.45 234 61 152 91 409.46 3.76 235 61 153 92 408.30 3.74 236 61 154 93 406.96 3.27 237 62 65 3 853.27 5.46 238 62 66 4 839.18 1.36 239 62 139 77 476.69 2.62 240 63 66 3 848.72 3.17 241 63 67 4 827.56 4.77 242 64 67 3 839.75 1.32 243 64 68 4 838.57 1.94 244 64 119 55 561.61 1.28 245 64 154 90 404.39 2.33 246 64 156 92 404.22 2.29 247 64 158 94 404.06 2.29 248 65 68 3 838.57 1.94 249 65 69 4 838.58 1.95 250 65 136 71 477.04 2.56 251 65 137 72 476.97 2.58 252 65 139 74 476.70 2.63 253 66 69 3 839.28 1.59 254 66 70 4 834.37 4.05 255 67 70 3 838.56 1.96 256 67 71 4 834.36 4.05 257 67 119 52 561.53 1.31 258 68 71 3 855.88 3.58 259 68 72 4 834.36 4.05 260 68 119 51 561.29 1.44 261 68 122 54 561.18 1.43 262 68 133 65 477.23 2.52 263 68 136 68 476.94 2.58 264 68 137 69 476.95 2.59 265 69 72 3 847.45 7.80 266 69 73 4 825.84 0.16 267 69 136 67 476.94 2.59 268 70 73 3 843.37 0.44 269 70 74 4 830.62 0.28 270 71 74 3 832.24 1.08 271 71 101 30 611.26 0.83 272 71 102 31 611.25 0.83 273 71 106 35 609.87 1.26 274 71 119 48 561.25 1.45 275 72 106 34 609.80 1.23 276 72 119 47 561.26 1.45 277 72 120 48 561.21 1.45 278 72 133 61 477.12 2.54 279 72 135 63 476.94 2.59 280 72 136 64 476.94 2.58 281 74 103 29 610.12 1.14 282 74 106 32 608.26 1.44 283 75 103 28 609.76 1.23 284 76 80 4 825.47 0.18 285 76 103 27 607.92 1.28 286 76 106 30 562.74 1.34 287 76 107 31 562.42 1.34 288 77 80 3 845.60 1.79 289 77 81 4 845.62 1.79 290 78 81 3 845.62 1.79 291 78 82 4 845.62 1.79 292 78 230 152 94.69 0.96 293 78 234 156 94.62 0.97 294 79 82 3 845.68 1.77 295 79 83 4 845.69 1.77 296 79 229 150 96.06 0.29 297 79 230 151 94.66 0.97 298 79 233 154 94.59 0.98 299 80 83 3 845.71 1.77 300 80 84 4 845.69 1.77 301 80 103 23 579.99 1.35 302 80 104 24 562.62 1.35 303 80 107 27 561.98 1.30 304 81 84 3 845.70 1.77 305 81 85 4 845.71 1.76 306 81 107 26 561.98 1.30 307 82 85 3 845.71 1.76 308 82 86 4 837.08 2.56 309 82 233 151 94.58 0.98 310 82 234 152 94.52 1.00 311 82 237 155 87.43 1.31 312 83 86 3 858.96 5.19 313 83 87 4 837.12 2.54 314 83 92 9 625.95 5.69 315 83 95 12 612.14 0.40 316 83 182 99 313.28 1.33 317 83 184 101 313.19 1.34 318 83 233 150 94.58 0.98 319 84 87 3 840.52 4.24 320 84 88 4 837.13 2.54 321 84 107 23 561.91 1.29 322 84 108 24 561.83 1.28 323 84 109 25 561.75 1.30 324 85 88 3 843.90 2.60 325 85 89 4 837.13 2.54 326 85 237 152 86.02 1.61 327 85 241 156 76.22 2.67 328 86 89 3 850.86 6.08 329 86 90 4 840.53 4.25 330 86 91 5 779.77 1.32 331 86 92 6 712.88 2.65 332 86 182 96 313.24 1.35 333 86 233 147 94.58 0.98 334 86 237 151 85.09 1.76 335 86 240 154 76.28 2.65 336 87 90 3 834.14 7.44 337 87 92 5 714.73 1.74 338 87 109 22 561.75 1.30 339 87 110 23 561.72 1.29 340 87 111 24 561.68 1.30 341 89 237 148 85.95 1.58 342 89 241 152 75.06 2.20 343 89 244 155 51.50 2.29 344 90 93 3 913.92 1.90 345 91 240 149 75.14 2.16 346 91 244 153 45.15 1.72 347 92 95 3 929.83 1.65 348 93 111 18 561.15 1.49 349 94 111 17 561.20 1.49 350 94 113 19 561.12 1.48 351 94 174 80 313.45 1.32 352 95 104 9 562.35 1.30 353 95 109 14 561.74 1.31 354 95 111 16 561.59 1.34 355 95 112 17 561.19 1.45 356 95 113 18 561.12 1.48 357 96 113 17 561.12 1.48 358 96 115 19 561.07 1.49 359 96 171 75 313.75 1.29 360 96 174 78 313.75 1.29 361 96 175 79 313.74 1.30 362 96 196 100 213.22 1.21 363 97 104 7 562.63 1.36 364 97 112 15 561.64 1.34 365 97 113 16 561.33 1.42 366 97 114 17 561.11 1.48 367 97 115 18 561.09 1.49 368 98 115 17 561.08 1.49 369 98 116 18 560.60 1.32 370 98 186 88 312.30 1.58 371 98 193 95 213.77 1.03 372 99 104 5 818.31 8.80 373 99 114 15 561.63 1.34 374 100 114 14 561.67 1.34 375 100 116 16 561.41 1.36 376 100 117 17 561.11 1.46 377 100 118 18 546.62 1.10 378 100 119 19 543.22 0.59 379 100 156 56 392.32 2.94 380 101 114 13 561.76 1.32 381 102 105 3 852.33 1.10 382 102 106 4 836.89 0.44 383 102 120 18 518.79 2.37 384 103 106 3 841.49 2.73 385 103 107 4 836.89 0.43 386 104 107 3 841.49 2.73 387 104 108 4 841.49 2.73 388 104 109 5 797.91 1.49 389 104 112 8 620.61 1.67 390 105 108 3 841.49 2.73 391 105 109 4 818.50 8.75 392 105 112 7 656.07 6.04 393 105 114 9 620.60 1.67 394 109 112 3 892.31 0.53 395 113 167 54 313.86 1.32 396 113 170 57 313.75 1.29 397 113 171 58 313.72 1.29 398 113 174 61 313.70 1.27 399 114 117 3 850.33 7.12 400 114 118 4 822.22 7.11 401 114 167 53 313.95 1.31 402 115 157 42 389.69 1.70 403 115 163 48 314.22 1.28 404 115 167 52 313.98 1.32 405 115 168 53 313.96 1.31 406 115 171 56 313.91 1.30 407 115 193 78 213.78 1.03 408 115 196 81 213.69 0.98 409 116 156 40 392.34 2.94 410 116 157 41 385.12 0.42 411 116 163 47 314.29 1.27 412 116 190 74 234.43 10.67 413 116 193 77 213.85 1.00 414 117 155 38 397.56 0.35 415 117 156 39 392.34 2.94 416 117 157 40 392.34 2.94 417 118 121 3 847.64 5.97 418 118 155 37 401.93 1.41 419 118 156 38 397.55 0.34 420 119 156 37 401.68 1.53 421 120 132 12 477.20 2.51 422 120 133 13 477.13 2.54 423 120 134 14 477.12 2.54 424 121 130 9 477.33 2.52 425 121 131 10 477.24 2.51 426 121 132 11 477.19 2.52 427 121 154 33 404.18 2.28 428 121 155 34 404.05 2.30 429 122 130 8 477.33 2.51 430 122 131 9 477.24 2.52 431 122 133 11 477.22 2.51 432 122 152 30 407.29 3.24 433 122 153 31 404.44 2.33 434 122 155 33 404.14 2.27 435 123 129 6 477.38 2.50 436 123 130 7 477.31 2.50 437 123 152 29 407.29 3.24 438 123 153 30 406.84 3.25 439 123 155 32 404.17 2.27 440 124 127 3 855.87 3.37 441 124 129 5 477.40 2.52 442 124 145 21 410.60 3.83 443 124 148 24 410.41 3.93 444 124 150 26 409.84 3.77 445 124 151 27 409.51 3.79 446 124 152 28 407.69 3.27 447 125 151 26 409.77 3.79 448 125 152 27 408.67 3.64 449 125 153 28 408.47 3.65 450 126 148 22 410.58 3.86 451 126 150 24 409.88 3.75 452 127 145 18 414.30 5.64 453 127 147 20 410.59 3.87 454 127 148 21 410.57 3.87 455 129 145 16 410.63 3.83 456 129 147 18 410.44 3.82 457 131 152 21 408.88 3.75 458 137 143 6 599.95 63.73 459 139 143 4 881.86 2.80 460 142 151 9 409.94 3.75 461 143 151 8 409.92 3.74 462 143 152 9 409.84 3.73 463 144 149 5 410.05 3.72 464 144 150 6 410.00 3.73 465 144 151 7 409.94 3.73 466 144 152 8 409.91 3.74 467 145 148 3 845.49 2.22 468 145 149 4 410.44 3.94 469 145 150 5 410.00 3.73 470 145 152 7 409.91 3.73 471 153 158 5 879.83 0.65 472 153 161 8 761.18 2.15 473 154 158 4 923.32 3.18 474 157 161 4 784.23 2.16 475 157 163 6 314.31 1.27 476 157 164 7 314.37 1.23 477 158 161 3 842.71 1.43 478 163 168 5 794.12 9.85 479 163 192 29 214.00 0.94 480 164 167 3 838.00 6.50 481 164 168 4 835.07 5.02 482 165 168 3 850.25 4.00 483 165 169 4 835.06 5.03 484 166 169 3 844.66 2.71 485 166 170 4 835.64 4.73 486 167 170 3 846.81 1.49 487 167 171 4 836.18 4.46 488 168 171 3 848.76 4.76 489 168 172 4 836.16 4.46 490 168 192 24 213.77 1.01 491 168 196 28 213.60 1.02 492 169 172 3 840.88 2.02 493 169 173 4 844.39 0.27 494 170 173 3 851.01 3.57 495 170 174 4 844.39 0.27 496 171 174 3 843.39 0.24 497 171 175 4 841.86 1.00 498 171 196 25 213.22 1.22 499 172 175 3 874.00 9.03 500 172 176 4 839.12 1.90 501 172 196 24 213.22 1.22 502 172 199 27 211.75 0.83 503 173 176 3 863.07 3.56 504 173 177 4 844.31 2.80 505 174 177 3 861.48 4.36 506 174 178 4 835.53 1.58 507 175 178 3 854.45 7.87 508 175 181 6 691.70 11.19 509 175 196 21 213.20 1.22 510 175 199 24 211.49 0.70 511 175 200 25 211.10 0.86 512 176 199 23 211.73 0.83 513 176 203 27 210.87 0.94 514 178 200 22 211.12 0.88 515 179 205 26 210.65 0.97 516 179 206 27 210.63 0.96 517 180 206 26 210.56 0.98 518 180 208 28 210.52 0.98 519 181 197 16 212.92 1.18 520 181 200 19 211.18 0.88 521 181 206 25 210.77 0.91 522 181 207 26 210.53 0.98 523 181 208 27 210.52 0.98 524 182 208 26 210.51 0.97 525 182 210 28 210.50 0.97 526 182 233 51 94.53 1.00 527 182 236 54 87.12 0.75 528 183 186 3 906.40 2.31 529 183 197 14 213.09 1.24 530 183 207 24 210.81 0.92 531 183 208 25 210.59 1.00 532 183 209 26 210.53 0.97 533 183 210 27 210.51 0.98 534 183 217 34 155.28 0.13 535 184 210 26 210.49 0.97 536 185 210 25 210.56 0.98 537 185 211 26 210.41 1.01 538 186 211 25 210.50 1.01 539 186 215 29 178.32 3.50 540 187 215 28 177.35 3.98 541 187 216 29 172.91 6.20 542 188 194 6 420.10 104.27 543 188 215 27 175.23 5.05 544 188 216 28 172.90 6.21 545 188 217 29 158.72 0.69 546 189 194 5 844.05 1.20 547 189 216 27 172.91 6.20 548 190 193 3 903.94 1.72 549 190 194 4 846.40 0.03 550 191 194 3 869.57 5.07 551 191 195 4 856.12 1.61 552 192 195 3 860.92 0.29 553 192 196 4 857.20 2.16 554 193 196 3 871.41 5.92 555 193 197 4 881.31 0.97 556 194 197 3 901.63 5.61 557 195 198 3 858.12 0.78 558 195 199 4 853.80 2.94 559 196 199 3 855.60 4.12 560 196 200 4 853.50 3.08 561 197 200 3 861.10 1.03 562 197 201 4 841.56 2.17 563 197 207 10 210.88 0.93 564 197 217 20 155.28 0.12 565 198 201 3 841.69 2.23 566 198 202 4 837.61 4.15 567 198 217 19 155.28 0.12 568 198 220 22 155.03 0.06 569 199 202 3 851.26 2.67 570 199 203 4 837.61 4.16 571 200 203 3 852.49 3.28 572 200 204 4 836.56 4.68 573 200 205 5 777.64 1.22 574 200 206 6 777.64 1.22 575 201 204 3 842.75 1.58 576 201 205 4 792.95 6.43 577 201 207 6 687.16 10.06 578 201 220 19 154.99 0.07 579 201 222 21 154.91 0.10 580 206 222 16 154.62 0.03 581 207 217 10 155.24 0.11 582 207 220 13 155.00 0.07 583 207 221 14 154.81 0.04 584 207 222 15 154.73 0.02 585 207 223 16 154.61 0.03 586 208 223 15 154.58 0.04 587 208 225 17 154.16 0.20 588 209 215 6 660.78 11.00 589 209 217 8 156.02 0.01 590 209 218 9 155.19 0.09 591 209 221 12 154.99 0.07 592 209 223 14 154.75 0.01 593 209 224 15 154.48 0.05 594 209 225 16 154.16 0.20 595 209 226 17 154.47 0.04 596 210 225 15 154.12 0.23 597 210 227 17 153.60 0.27 598 210 229 19 97.84 0.62 599 210 232 22 94.62 0.96 600 210 233 23 94.64 0.96 601 211 215 4 856.45 0.61 602 211 225 14 154.63 0.06 603 211 226 15 154.06 0.21 604 211 227 16 154.02 0.20 605 212 226 14 154.50 0.05 606 212 227 15 154.05 0.21 607 213 226 13 154.72 0.03 608 214 218 4 819.88 0.54 609 214 226 12 154.83 0.06 610 215 218 3 849.86 0.73 611 215 219 4 864.77 0.18 612 216 219 3 900.60 3.15 613 217 220 3 878.36 3.24 614 217 221 4 840.53 1.85 615 218 221 3 847.28 0.17 616 218 224 6 154.97 0.08 617 218 226 8 403.87 124.38 618 221 224 3 852.39 3.12 619 225 232 7 372.41 137.93 620 227 232 5 788.61 11.68 621 228 231 3 862.69 4.44 622 228 232 4 822.60 0.73 623 229 232 3 850.54 1.64 624 229 233 4 819.76 0.69 625 230 233 3 852.07 0.41 626 230 234 4 824.97 1.90 627 231 234 3 849.99 4.16 628 231 235 4 826.85 0.61 629 232 235 3 849.31 3.80 630 232 236 4 818.80 3.41 631 233 236 3 837.16 2.29 632 233 237 4 818.79 3.40 633 234 237 3 852.34 5.29 634 234 238 4 829.36 1.95 635 235 238 3 835.27 1.01 636 235 239 4 829.35 1.96 637 236 239 3 841.28 2.00 638 236 240 4 814.00 1.88 639 237 240 3 843.71 3.57 640 237 241 4 814.81 1.54 641 238 241 3 843.71 3.57 642 238 242 4 814.83 1.54 643 239 242 3 831.89 2.83 644 239 243 4 825.94 3.89 645 240 243 3 832.82 2.38 646 240 244 4 831.28 1.22 647 241 244 3 847.96 8.33 648 241 245 4 824.78 2.04 649 242 245 3 834.59 1.80 650 242 246 4 824.00 2.43 651 243 246 3 863.77 1.27 652 243 247 4 845.05 8.10 653 244 247 3 847.80 6.72