BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 232 231 33.66 0.14 2 1 235 234 24.97 0.94 3 1 236 235 23.77 0.34 4 2 5 3 862.72 9.90 5 2 6 4 833.50 3.24 6 3 6 3 864.97 5.57 7 3 7 4 833.68 3.35 8 4 7 3 833.71 3.33 9 4 8 4 834.33 3.67 10 4 223 219 80.00 0.98 11 5 8 3 850.69 1.56 12 5 9 4 840.39 6.71 13 5 208 203 79.97 0.99 14 5 223 218 76.34 2.81 15 5 225 220 75.92 2.60 16 5 236 231 59.53 0.59 17 6 9 3 840.44 6.70 18 6 10 4 833.43 3.23 19 6 236 230 60.38 1.01 20 6 239 233 44.67 1.24 21 7 10 3 840.21 6.58 22 7 11 4 818.01 0.79 23 8 12 4 800.49 7.98 24 8 180 172 158.42 36.28 25 8 206 198 83.36 1.21 26 8 208 200 83.09 1.19 27 8 223 215 83.17 1.28 28 9 12 3 840.27 0.72 29 9 15 6 663.59 4.38 30 9 86 77 410.17 28.23 31 9 91 82 399.39 33.62 32 9 180 171 158.45 36.27 33 9 182 173 83.09 1.19 34 9 208 199 83.22 1.25 35 9 236 227 65.05 0.97 36 9 240 231 53.96 0.95 37 10 240 230 53.29 1.29 38 10 243 233 40.60 0.27 39 10 244 234 39.87 0.42 40 12 15 3 861.55 0.43 41 12 91 79 627.63 1.27 42 12 179 167 313.56 0.70 43 12 180 168 275.45 0.85 44 13 93 80 628.19 1.05 45 13 179 166 314.18 0.69 46 14 93 79 628.18 1.05 47 14 174 160 314.60 0.78 48 14 177 163 314.49 0.74 49 14 178 164 314.38 0.78 50 14 179 165 314.31 0.75 51 15 86 71 628.36 1.05 52 15 91 76 628.31 1.07 53 15 92 77 628.23 1.04 54 15 93 78 628.21 1.05 55 15 179 164 314.45 0.75 56 15 180 165 314.20 0.70 57 15 182 167 314.20 0.70 58 16 92 76 628.27 1.05 59 16 94 78 628.21 1.05 60 16 174 158 314.72 0.77 61 16 175 159 314.66 0.77 62 16 178 162 314.54 0.76 63 16 180 164 314.48 0.76 64 16 181 165 314.31 0.75 65 16 182 166 314.30 0.74 66 17 83 66 628.35 1.04 67 17 92 75 628.35 1.06 68 17 94 77 628.28 1.06 69 17 95 78 628.20 1.05 70 17 96 79 628.19 1.05 71 17 181 164 314.42 0.77 72 17 182 165 314.31 0.75 73 18 96 78 628.19 1.05 74 18 97 79 628.19 1.05 75 18 98 80 628.19 1.05 76 18 115 97 560.83 1.62 77 18 181 163 314.51 0.76 78 18 182 164 314.48 0.76 79 18 183 165 314.31 0.75 80 18 184 166 314.31 0.76 81 18 193 175 213.91 1.43 82 18 196 178 213.52 1.55 83 18 197 179 213.41 1.54 84 19 23 4 825.89 0.07 85 19 83 64 628.56 1.08 86 19 95 76 628.31 1.06 87 19 96 77 628.26 1.06 88 19 97 78 628.20 1.05 89 19 98 79 628.19 1.05 90 19 104 85 562.58 1.44 91 19 184 165 314.31 0.76 92 20 23 3 876.88 3.85 93 20 98 78 628.21 1.05 94 20 99 79 628.16 1.04 95 20 184 164 314.45 0.77 96 20 185 165 314.18 0.70 97 20 186 166 311.57 1.54 98 21 24 3 858.61 1.93 99 21 25 4 837.26 1.87 100 21 31 10 630.54 1.11 101 21 32 11 630.56 1.10 102 21 99 78 628.21 1.05 103 21 185 164 314.44 0.77 104 22 25 3 844.63 4.28 105 22 31 9 631.53 1.58 106 22 185 163 314.42 0.76 107 23 77 54 629.77 1.11 108 23 79 56 629.10 0.99 109 23 80 57 629.10 0.99 110 23 83 60 629.09 0.98 111 23 104 81 563.98 1.01 112 23 184 161 314.48 0.76 113 23 185 162 314.35 0.78 114 23 229 206 98.31 4.44 115 24 27 3 844.65 4.27 116 24 28 4 826.62 4.73 117 24 29 5 678.69 7.87 118 24 77 53 629.77 1.11 119 24 80 56 629.32 0.99 120 24 101 77 628.19 1.05 121 24 103 79 600.41 1.14 122 25 28 3 858.92 0.73 123 25 29 4 719.29 12.19 124 25 31 6 638.01 1.39 125 26 77 51 629.95 1.05 126 26 228 202 134.19 2.41 127 26 229 203 134.17 2.40 128 26 230 204 126.59 1.22 129 27 74 47 630.06 1.05 130 27 75 48 630.04 1.05 131 27 76 49 630.00 1.03 132 27 77 50 629.96 1.05 133 27 80 53 629.96 1.05 134 27 103 76 627.62 0.92 135 29 67 38 630.54 1.06 136 29 71 42 630.25 1.13 137 29 74 45 630.12 1.06 138 29 103 74 628.15 1.06 139 30 36 6 647.31 0.67 140 30 38 8 647.28 0.66 141 30 67 37 630.57 1.06 142 31 37 6 647.30 0.67 143 31 38 7 647.33 0.66 144 32 37 5 647.32 0.67 145 32 38 6 647.34 0.66 146 32 39 7 647.34 0.65 147 32 40 8 647.37 0.67 148 33 37 4 647.37 0.66 149 33 39 6 655.16 4.56 150 33 40 7 647.38 0.66 151 33 159 126 414.52 1.89 152 33 187 154 314.50 0.78 153 33 189 156 314.47 0.79 154 33 190 157 314.38 0.75 155 34 37 3 811.66 5.08 156 34 40 6 686.25 4.70 157 37 40 3 860.12 0.86 158 37 41 4 826.06 3.26 159 38 41 3 862.71 0.42 160 38 42 4 796.98 0.41 161 38 63 25 634.31 0.59 162 38 67 29 630.68 1.06 163 39 42 3 830.94 0.82 164 39 44 5 750.35 6.54 165 39 48 9 675.06 10.46 166 39 63 24 634.45 0.64 167 39 159 120 414.76 1.79 168 42 47 5 791.23 3.98 169 42 63 21 634.46 0.64 170 42 66 24 634.41 0.61 171 43 46 3 856.52 1.96 172 43 47 4 827.73 1.59 173 44 47 3 845.69 7.38 174 44 48 4 791.21 3.98 175 44 49 5 791.21 3.98 176 44 159 115 415.48 2.08 177 45 49 4 815.06 7.92 178 45 52 7 763.89 0.01 179 46 49 3 891.32 1.07 180 46 52 6 763.88 0.01 181 46 59 13 664.28 4.59 182 46 60 14 637.27 0.09 183 47 52 5 773.72 4.27 184 47 59 12 646.27 4.41 185 47 60 13 634.86 0.69 186 47 63 16 634.78 0.71 187 47 64 17 634.80 0.70 188 47 158 111 415.54 2.09 189 48 158 110 415.52 2.10 190 48 159 111 415.50 2.08 191 49 52 3 853.66 2.64 192 49 53 4 833.74 0.03 193 49 158 109 415.54 2.10 194 50 53 3 844.32 5.32 195 50 54 4 837.31 1.81 196 50 158 108 415.73 2.07 197 50 159 109 415.71 2.06 198 50 161 111 415.46 2.06 199 51 54 3 859.18 0.82 200 51 55 4 837.80 3.76 201 52 55 3 846.81 0.74 202 52 56 4 829.53 2.12 203 52 60 8 760.13 4.83 204 53 56 3 874.09 3.08 205 53 57 4 786.55 2.19 206 53 60 7 758.68 3.14 207 53 61 8 775.99 3.08 208 53 125 72 559.73 1.85 209 53 151 98 421.01 0.68 210 53 152 99 420.97 0.66 211 53 153 100 420.24 0.35 212 53 158 105 415.75 2.06 213 54 57 3 875.91 2.17 214 56 59 3 855.32 0.86 215 56 60 4 832.98 0.90 216 57 60 3 849.55 2.02 217 57 61 4 832.98 0.90 218 57 142 85 472.43 4.49 219 57 151 94 421.01 0.68 220 58 61 3 835.22 0.17 221 58 62 4 835.15 0.20 222 58 139 81 473.08 4.21 223 58 140 82 472.94 4.27 224 58 142 84 472.36 4.45 225 59 62 3 849.33 6.89 226 59 63 4 831.35 2.10 227 60 63 3 848.91 4.12 228 60 64 4 847.31 3.87 229 60 158 98 415.75 2.04 230 61 64 3 847.32 3.87 231 61 65 4 845.50 4.77 232 61 68 7 828.85 2.91 233 61 122 61 561.10 1.59 234 61 137 76 473.50 4.19 235 61 139 78 472.50 4.49 236 61 142 81 471.51 4.12 237 61 143 82 463.47 0.47 238 61 151 90 420.97 0.66 239 61 152 91 420.63 0.51 240 61 153 92 419.98 0.47 241 61 154 93 416.70 1.73 242 62 65 3 851.88 6.15 243 62 66 4 847.30 3.88 244 62 139 77 472.50 4.48 245 63 66 3 848.46 3.29 246 63 67 4 838.33 0.61 247 64 67 3 838.33 0.61 248 64 68 4 837.11 1.22 249 64 119 55 561.24 1.53 250 64 154 90 415.75 2.06 251 64 156 92 415.69 2.07 252 64 158 94 415.49 2.08 253 65 68 3 838.18 0.68 254 65 69 4 837.10 1.22 255 65 136 71 473.11 4.26 256 65 137 72 472.64 4.49 257 65 139 74 472.50 4.49 258 66 69 3 840.75 0.60 259 66 70 4 835.82 1.85 260 67 70 3 835.32 0.34 261 67 71 4 828.86 1.64 262 67 119 52 560.98 1.60 263 68 71 3 848.35 7.10 264 68 72 4 828.82 1.62 265 68 119 51 560.98 1.60 266 68 122 54 560.86 1.61 267 68 133 65 473.55 4.19 268 68 136 68 472.60 4.51 269 68 137 69 472.61 4.50 270 69 72 3 848.35 7.10 271 69 73 4 828.57 1.76 272 69 136 67 472.59 4.51 273 70 73 3 841.77 1.24 274 70 74 4 828.12 1.53 275 71 74 3 828.12 1.53 276 71 101 30 628.32 1.05 277 71 102 31 628.32 1.05 278 71 106 35 627.40 0.80 279 71 119 48 560.90 1.62 280 72 106 34 627.23 0.64 281 72 119 47 561.00 1.59 282 72 120 48 560.89 1.62 283 72 133 61 473.40 4.19 284 72 135 63 472.60 4.51 285 72 136 64 472.59 4.51 286 74 103 29 627.74 0.85 287 74 106 32 624.15 0.18 288 75 103 28 625.34 0.21 289 76 80 4 804.63 15.50 290 76 103 27 607.62 2.10 291 76 106 30 562.70 1.44 292 76 107 31 562.54 1.47 293 77 80 3 848.70 3.50 294 77 81 4 824.59 8.58 295 78 81 3 845.47 1.86 296 78 82 4 845.47 1.86 297 78 230 152 69.71 0.28 298 78 234 156 69.21 0.48 299 79 82 3 845.47 1.86 300 79 83 4 845.47 1.86 301 79 229 150 89.66 7.43 302 79 230 151 69.68 0.27 303 79 233 154 69.19 0.49 304 80 83 3 845.48 1.86 305 80 84 4 845.47 1.87 306 80 103 23 562.69 1.45 307 80 104 24 562.63 1.47 308 80 107 27 561.81 1.51 309 81 84 3 845.39 1.92 310 81 85 4 845.39 1.92 311 81 107 26 561.79 1.51 312 82 85 3 845.39 1.92 313 82 86 4 845.38 1.93 314 82 233 151 69.09 0.53 315 82 234 152 68.53 0.80 316 82 237 155 68.17 0.62 317 83 86 3 859.02 4.90 318 83 87 4 843.65 2.78 319 83 92 9 671.78 17.48 320 83 95 12 628.36 1.07 321 83 104 21 562.57 1.45 322 83 182 99 314.48 0.76 323 83 184 101 314.41 0.74 324 83 233 150 69.09 0.53 325 84 87 3 855.87 3.33 326 84 88 4 825.99 6.05 327 84 107 23 561.81 1.52 328 84 108 24 561.59 1.52 329 84 109 25 561.38 1.57 330 85 88 3 833.61 3.87 331 85 89 4 828.69 4.70 332 85 237 152 66.98 0.06 333 85 241 156 65.06 0.82 334 86 89 3 850.61 5.96 335 86 90 4 830.32 5.51 336 86 91 5 780.19 3.35 337 86 92 6 684.72 11.18 338 86 182 96 314.44 0.74 339 86 233 147 69.08 0.53 340 86 237 151 66.89 0.10 341 86 240 154 59.86 0.95 342 87 90 3 830.31 5.52 343 87 92 5 740.84 11.33 344 87 109 22 561.38 1.56 345 87 110 23 561.36 1.55 346 87 111 24 561.26 1.54 347 89 237 148 66.94 0.07 348 89 241 152 55.84 0.69 349 89 244 155 51.35 2.12 350 90 93 3 913.67 1.78 351 91 240 149 59.50 0.79 352 91 244 153 46.35 0.83 353 92 95 3 931.63 0.49 354 93 111 18 560.96 1.66 355 94 111 17 560.96 1.66 356 94 113 19 560.88 1.63 357 94 174 80 314.60 0.78 358 95 104 9 562.19 1.39 359 95 111 16 561.11 1.61 360 95 112 17 560.89 1.63 361 95 113 18 560.88 1.63 362 96 113 17 560.90 1.62 363 96 115 19 560.82 1.62 364 96 171 75 314.97 0.83 365 96 174 78 314.97 0.83 366 96 175 79 314.95 0.83 367 96 196 100 213.53 1.55 368 97 104 7 562.63 1.46 369 97 112 15 561.11 1.62 370 97 113 16 560.96 1.65 371 97 114 17 560.85 1.61 372 97 115 18 560.83 1.61 373 98 115 17 560.84 1.61 374 98 116 18 560.61 1.58 375 98 186 88 313.63 0.91 376 98 193 95 214.22 1.42 377 99 114 15 561.09 1.61 378 100 114 14 561.15 1.60 379 100 116 16 561.00 1.61 380 100 117 17 560.84 1.60 381 100 118 18 544.77 1.25 382 100 119 19 538.29 1.88 383 100 156 56 384.94 0.00 384 101 114 13 561.52 1.54 385 102 105 3 846.37 4.08 386 102 106 4 833.49 2.14 387 102 120 18 517.44 2.79 388 103 106 3 837.99 0.11 389 103 107 4 820.05 4.58 390 104 107 3 849.76 1.40 391 104 108 4 833.48 2.14 392 104 109 5 797.47 1.71 393 104 112 8 594.01 17.80 394 105 108 3 836.60 5.19 395 105 109 4 797.56 1.66 396 105 112 7 656.85 1.01 397 105 114 9 598.98 20.27 398 109 112 3 892.06 0.41 399 113 167 54 314.98 0.83 400 113 170 57 314.97 0.83 401 113 171 58 314.95 0.83 402 113 174 61 314.95 0.83 403 114 117 3 849.91 7.33 404 114 118 4 835.85 0.29 405 114 167 53 315.17 0.81 406 115 157 42 384.86 0.01 407 115 163 48 315.60 0.76 408 115 167 52 315.17 0.81 409 115 168 53 315.16 0.80 410 115 171 56 315.12 0.79 411 115 193 78 214.16 1.39 412 115 196 81 214.18 1.40 413 116 156 40 384.93 0.01 414 116 157 41 384.26 0.24 415 116 163 47 315.63 0.76 416 116 190 74 243.56 6.33 417 116 193 77 214.27 1.44 418 117 155 38 397.36 0.64 419 117 156 39 384.92 0.01 420 117 157 40 384.92 0.01 421 118 121 3 847.38 5.85 422 118 155 37 398.03 0.30 423 118 156 38 392.73 0.04 424 119 156 37 401.68 1.52 425 120 132 12 473.51 4.19 426 120 133 13 473.48 4.21 427 120 134 14 473.36 4.26 428 121 130 9 473.81 4.23 429 121 131 10 473.76 4.22 430 121 132 11 473.54 4.17 431 121 154 33 415.43 2.06 432 121 155 34 415.39 2.06 433 122 130 8 473.82 4.22 434 122 131 9 473.79 4.24 435 122 133 11 473.67 4.22 436 122 153 31 415.75 2.06 437 122 155 33 415.42 2.05 438 123 129 6 473.84 4.23 439 123 130 7 473.82 4.22 440 123 152 29 419.66 0.32 441 123 153 30 416.51 1.75 442 123 155 32 415.43 2.05 443 124 127 3 855.62 3.24 444 124 129 5 473.85 4.23 445 124 145 21 421.56 0.38 446 124 148 24 421.65 0.42 447 124 150 26 420.50 0.42 448 124 151 27 420.42 0.40 449 124 152 28 419.67 0.32 450 125 151 26 420.44 0.40 451 125 152 27 420.41 0.40 452 125 153 28 420.03 0.21 453 126 148 22 421.75 0.41 454 126 150 24 421.13 0.59 455 127 145 18 423.35 1.10 456 127 147 20 421.79 0.41 457 127 148 21 421.73 0.42 458 129 145 16 421.72 0.36 459 129 147 18 421.63 0.40 460 131 152 21 419.99 0.26 461 137 143 6 468.45 7.08 462 139 143 4 882.33 1.53 463 142 151 9 421.01 0.68 464 143 151 8 421.48 0.45 465 143 152 9 420.62 0.64 466 144 149 5 421.71 0.48 467 144 150 6 421.63 0.51 468 144 152 8 420.74 0.70 469 145 148 3 850.66 0.37 470 145 149 4 421.85 0.42 471 145 150 5 421.84 0.41 472 145 152 7 420.85 0.77 473 153 158 5 879.58 0.77 474 153 161 8 744.99 10.25 475 154 158 4 923.07 3.06 476 157 161 4 801.51 6.73 477 157 163 6 315.67 0.76 478 157 164 7 315.67 0.76 479 158 161 3 838.19 0.58 480 162 165 3 952.21 2.18 481 163 168 5 814.00 9.27 482 163 192 29 214.40 1.39 483 164 167 3 851.57 3.33 484 164 168 4 843.42 7.41 485 165 168 3 849.11 5.19 486 165 169 4 827.70 2.46 487 166 169 3 849.12 5.19 488 166 170 4 844.91 0.01 489 167 170 3 854.22 0.29 490 167 171 4 844.92 0.00 491 168 171 3 851.53 3.31 492 168 172 4 840.62 2.15 493 168 192 24 214.19 1.40 494 168 196 28 213.99 1.40 495 169 172 3 842.17 2.92 496 169 173 4 840.62 2.15 497 170 173 3 855.81 5.87 498 170 174 4 847.24 5.46 499 171 174 3 848.11 1.57 500 171 175 4 841.61 0.87 501 171 196 25 213.54 1.55 502 172 175 3 873.75 9.15 503 172 176 4 837.37 1.25 504 172 196 24 213.62 1.52 505 172 199 27 212.28 0.95 506 173 176 3 862.82 3.68 507 173 177 4 837.37 1.25 508 174 177 3 865.08 2.56 509 174 178 4 829.57 1.40 510 175 178 3 861.23 4.48 511 175 181 6 678.57 13.35 512 175 196 21 213.50 1.54 513 175 199 24 211.49 1.27 514 175 200 25 211.46 1.27 515 176 199 23 211.52 1.26 516 176 203 27 210.75 1.47 517 178 200 22 211.33 1.28 518 179 205 26 210.63 1.47 519 179 206 27 210.63 1.47 520 180 200 20 211.47 1.28 521 180 206 26 210.56 1.51 522 180 208 28 210.53 1.50 523 181 197 16 213.24 1.54 524 181 200 19 211.60 1.33 525 181 206 25 210.74 1.48 526 181 207 26 210.55 1.50 527 181 208 27 210.51 1.52 528 182 208 26 210.50 1.51 529 182 210 28 210.42 1.51 530 182 233 51 68.52 0.80 531 182 236 54 66.81 0.14 532 183 186 3 909.87 4.29 533 183 197 14 213.39 1.53 534 183 207 24 210.75 1.48 535 183 208 25 210.67 1.51 536 183 209 26 210.44 1.51 537 183 210 27 210.44 1.51 538 183 217 34 155.37 0.18 539 184 210 26 210.42 1.51 540 185 210 25 210.44 1.51 541 185 211 26 210.41 1.51 542 186 211 25 210.42 1.51 543 186 215 29 168.78 1.02 544 187 215 28 165.29 0.72 545 187 216 29 162.02 2.37 546 188 194 6 214.09 1.52 547 188 215 27 162.02 2.37 548 188 216 28 162.00 2.37 549 188 217 29 161.88 2.35 550 189 194 5 820.38 5.13 551 189 216 27 162.00 2.36 552 190 193 3 904.89 0.99 553 190 194 4 851.12 0.64 554 191 194 3 869.32 4.95 555 191 195 4 855.86 1.74 556 192 195 3 863.93 1.22 557 192 196 4 856.95 2.28 558 193 196 3 881.06 1.09 559 193 197 4 881.06 1.09 560 194 197 3 891.54 3.46 561 195 198 3 870.19 4.34 562 195 199 4 848.20 0.71 563 196 199 3 863.12 0.12 564 196 200 4 856.15 3.26 565 197 200 3 860.85 0.91 566 197 201 4 840.32 2.93 567 197 207 10 210.75 1.49 568 197 217 20 155.36 0.20 569 198 201 3 850.97 2.81 570 198 202 4 830.22 0.89 571 198 217 19 155.37 0.20 572 198 220 22 154.75 0.01 573 199 202 3 852.19 3.43 574 199 203 4 835.74 3.68 575 200 203 3 851.78 3.63 576 200 204 4 833.41 4.84 577 200 205 5 733.09 6.55 578 200 206 6 719.88 0.04 579 201 204 3 830.81 4.39 580 201 205 4 792.73 8.04 581 201 207 6 684.89 7.62 582 201 220 19 154.74 0.00 583 201 222 21 154.62 0.01 584 206 222 16 154.41 0.07 585 207 217 10 155.35 0.19 586 207 220 13 154.77 0.03 587 207 221 14 154.53 0.07 588 207 222 15 154.46 0.10 589 207 223 16 154.40 0.07 590 208 223 15 154.36 0.06 591 208 225 17 154.00 0.10 592 209 215 6 437.51 135.47 593 209 217 8 156.18 0.21 594 209 218 9 155.24 0.16 595 209 221 12 154.76 0.04 596 209 223 14 154.52 0.06 597 209 224 15 154.05 0.09 598 209 225 16 153.94 0.14 599 209 226 17 153.88 0.10 600 210 225 15 153.88 0.10 601 210 227 17 153.28 0.11 602 210 229 19 82.70 3.39 603 210 232 22 69.05 0.55 604 211 215 4 856.20 0.49 605 211 225 14 154.38 0.06 606 211 226 15 153.87 0.10 607 211 227 16 153.59 0.01 608 212 226 14 154.34 0.07 609 212 227 15 153.78 0.06 610 213 226 13 154.50 0.04 611 214 218 4 834.67 4.53 612 214 226 12 154.63 0.01 613 215 218 3 849.59 0.60 614 215 219 4 872.34 3.97 615 216 219 3 900.35 3.27 616 217 220 3 878.11 3.12 617 217 221 4 832.45 0.70 618 218 221 3 845.72 0.95 619 218 224 6 154.66 0.01 620 218 226 8 154.66 0.01 621 221 224 3 857.56 0.55 622 225 232 7 69.05 0.55 623 227 232 5 742.62 1.50 624 228 231 3 862.44 4.57 625 228 232 4 801.81 5.20 626 229 232 3 847.51 2.90 627 229 233 4 801.80 5.20 628 230 234 4 813.18 7.79 629 231 234 3 851.46 0.71 630 231 235 4 819.18 3.21 631 232 235 3 848.24 4.08 632 232 236 4 819.22 4.77 633 233 236 3 833.02 2.13 634 233 237 4 817.57 3.95 635 234 237 3 849.94 6.25 636 234 238 4 818.18 3.64 637 235 238 3 835.12 1.19 638 235 239 4 820.57 2.44 639 236 239 3 843.88 3.29 640 236 240 4 823.56 0.93 641 237 240 3 838.91 5.72 642 237 241 4 810.88 5.34 643 238 241 3 843.27 3.54 644 238 242 4 814.51 1.65 645 239 242 3 839.00 0.97 646 239 243 4 827.61 4.73 647 240 243 3 840.55 2.18 648 240 244 4 820.05 4.41 649 241 244 3 837.17 3.87 650 241 245 4 824.53 2.16 651 242 245 3 836.98 0.36 652 242 246 4 824.53 2.16 653 243 246 3 863.52 1.40 654 243 247 4 863.52 1.40 655 244 247 3 863.52 1.40