BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 232 231 31.66 1.15 2 1 235 234 28.98 2.48 3 1 236 235 28.96 2.48 4 1 239 238 27.96 2.34 5 2 5 3 849.59 6.45 6 2 6 4 839.20 1.25 7 3 6 3 859.56 2.87 8 3 7 4 834.32 3.69 9 4 7 3 844.78 4.04 10 4 8 4 834.34 3.68 11 4 223 219 60.90 2.00 12 5 8 3 841.82 0.07 13 5 9 4 834.33 3.68 14 5 208 203 60.68 2.00 15 5 223 218 60.68 2.00 16 5 225 220 60.67 2.00 17 5 236 231 50.04 1.59 18 6 9 3 840.40 6.70 19 6 10 4 831.24 4.62 20 6 236 230 58.55 1.79 21 6 239 233 38.59 0.07 22 6 240 234 38.59 0.07 23 7 10 3 839.85 6.76 24 7 11 4 819.22 1.39 25 8 12 4 800.49 7.98 26 8 180 172 62.62 1.67 27 8 206 198 62.63 1.67 28 8 208 200 61.72 1.99 29 8 223 215 61.45 1.85 30 9 12 3 840.27 0.72 31 9 15 6 633.13 5.33 32 9 91 82 343.94 6.03 33 9 208 199 62.05 1.84 34 9 236 227 60.48 1.93 35 9 240 231 53.76 0.71 36 10 240 230 53.30 1.28 37 10 243 233 42.70 1.82 38 10 244 234 42.62 1.79 39 12 15 3 861.55 0.43 40 12 91 79 611.00 0.83 41 12 179 167 312.58 1.42 42 12 180 168 283.07 1.27 43 13 93 80 611.04 0.83 44 13 179 166 312.69 1.44 45 14 93 79 611.04 0.83 46 14 174 160 313.32 1.41 47 14 177 163 313.14 1.42 48 14 178 164 312.99 1.48 49 14 179 165 312.81 1.41 50 15 86 71 611.29 0.84 51 15 91 76 611.18 0.84 52 15 92 77 611.12 0.86 53 15 93 78 611.10 0.85 54 15 179 164 313.11 1.42 55 15 180 165 312.71 1.44 56 15 182 167 312.72 1.44 57 16 92 76 611.18 0.84 58 16 94 78 611.08 0.84 59 16 174 158 313.43 1.41 60 16 175 159 313.39 1.41 61 16 178 162 313.20 1.42 62 16 180 164 313.12 1.43 63 16 181 165 312.81 1.42 64 16 182 166 312.80 1.42 65 17 83 66 611.34 0.88 66 17 92 75 611.25 0.83 67 17 94 77 611.18 0.85 68 17 95 78 611.07 0.84 69 17 96 79 611.03 0.83 70 17 181 164 313.04 1.46 71 17 182 165 312.82 1.41 72 18 96 78 611.07 0.84 73 18 98 80 611.03 0.83 74 18 115 97 560.14 1.96 75 18 181 163 313.20 1.42 76 18 182 164 313.07 1.45 77 18 183 165 312.73 1.44 78 18 184 166 312.72 1.44 79 18 193 175 213.70 1.60 80 18 196 178 213.40 1.62 81 18 197 179 213.27 1.60 82 19 23 4 832.56 3.27 83 19 83 64 611.44 0.84 84 19 95 76 611.24 0.84 85 19 96 77 611.15 0.84 86 19 97 78 611.05 0.84 87 19 98 79 611.05 0.84 88 19 104 85 561.32 1.85 89 19 184 165 312.74 1.44 90 20 23 3 876.88 3.85 91 20 98 78 611.07 0.85 92 20 99 79 611.03 0.83 93 20 184 164 313.07 1.46 94 20 185 165 312.69 1.44 95 20 186 166 311.08 1.78 96 21 24 3 859.50 2.38 97 21 25 4 832.75 4.13 98 21 31 10 613.87 0.91 99 21 32 11 613.87 0.91 100 21 99 78 611.06 0.84 101 21 185 164 313.06 1.47 102 22 25 3 843.35 4.92 103 22 31 9 613.88 0.90 104 22 185 163 313.03 1.47 105 23 77 54 612.56 0.97 106 23 79 56 612.06 0.75 107 23 80 57 612.06 0.75 108 23 83 60 612.06 0.75 109 23 104 81 584.67 2.71 110 23 184 161 313.18 1.42 111 23 185 162 312.94 1.48 112 23 229 206 100.80 1.83 113 24 27 3 841.13 2.53 114 24 29 5 652.89 9.10 115 24 77 53 612.59 0.98 116 24 80 56 612.54 0.96 117 24 99 75 611.15 0.83 118 24 101 77 610.84 0.78 119 24 103 79 601.65 1.75 120 25 28 3 858.92 0.73 121 25 29 4 658.84 9.55 122 25 31 6 623.48 3.81 123 26 77 51 612.92 0.93 124 26 228 202 135.57 3.10 125 26 229 203 134.34 2.49 126 26 230 204 128.66 2.25 127 27 74 47 613.06 0.93 128 27 75 48 613.00 0.95 129 27 76 49 612.99 0.94 130 27 77 50 612.92 0.93 131 27 80 53 612.93 0.93 132 27 103 76 609.88 1.01 133 29 67 38 613.47 0.93 134 29 70 41 613.31 0.90 135 29 71 42 613.27 0.91 136 29 74 45 613.14 0.91 137 29 103 74 610.91 0.81 138 30 36 6 646.93 1.63 139 30 38 8 646.88 1.62 140 30 67 37 613.58 0.97 141 31 37 6 646.93 1.62 142 31 38 7 646.92 1.62 143 32 37 5 646.94 1.64 144 32 38 6 646.90 1.64 145 32 39 7 646.92 1.64 146 32 40 8 646.93 1.64 147 33 37 4 646.95 1.64 148 33 39 6 646.95 1.64 149 33 40 7 646.94 1.64 150 33 159 126 406.35 6.38 151 33 187 154 313.14 1.46 152 33 189 156 313.14 1.46 153 33 190 157 312.94 1.46 154 34 37 3 840.96 13.98 155 34 40 6 671.00 9.54 156 37 40 3 851.16 5.34 157 37 41 4 814.92 1.67 158 38 41 3 854.63 3.60 159 38 42 4 807.51 4.85 160 38 63 25 620.91 1.33 161 38 67 29 613.61 0.98 162 39 42 3 830.94 0.82 163 39 44 5 685.82 2.13 164 39 48 9 671.38 9.39 165 39 63 24 621.16 1.40 166 39 159 120 406.50 6.34 167 42 47 5 814.94 7.98 168 42 63 21 621.13 1.36 169 42 66 24 621.03 1.32 170 43 46 3 856.52 1.96 171 43 47 4 832.86 0.97 172 44 47 3 845.67 7.38 173 44 48 4 790.40 0.01 174 44 49 5 790.44 0.00 175 44 159 115 406.81 6.41 176 45 49 4 823.38 3.76 177 45 52 7 681.40 5.29 178 46 52 6 679.02 6.40 179 46 59 13 624.83 2.19 180 46 60 14 624.81 2.18 181 47 52 5 692.50 0.26 182 47 59 12 624.74 2.23 183 47 60 13 624.57 2.29 184 47 63 16 624.55 2.28 185 47 64 17 624.54 2.29 186 48 64 16 624.57 2.29 187 48 158 110 407.61 6.03 188 48 159 111 407.55 6.05 189 49 52 3 843.44 7.75 190 49 53 4 829.11 1.50 191 49 158 109 408.87 5.42 192 50 53 3 837.74 2.02 193 50 54 4 833.10 3.91 194 50 158 108 409.48 5.25 195 50 159 109 409.40 5.23 196 50 161 111 407.63 5.98 197 51 54 3 855.11 1.22 198 51 55 4 833.12 3.90 199 52 55 3 834.38 1.14 200 52 56 4 825.71 0.21 201 52 60 8 761.59 4.12 202 53 56 3 874.09 3.08 203 53 57 4 794.00 11.69 204 53 60 7 763.14 3.35 205 53 61 8 763.54 3.54 206 53 125 72 558.54 2.37 207 53 152 99 411.44 4.86 208 53 153 100 411.15 4.90 209 53 154 101 410.74 5.11 210 53 158 105 409.50 5.24 211 54 57 3 875.92 2.17 212 56 59 3 855.32 0.86 213 56 60 4 832.94 0.90 214 57 60 3 839.09 3.21 215 57 61 4 823.16 3.99 216 57 142 85 476.20 2.60 217 57 151 94 411.56 4.83 218 58 61 3 833.02 0.93 219 58 62 4 827.78 1.69 220 58 139 81 476.53 2.48 221 58 140 82 476.51 2.48 222 58 142 84 476.18 2.61 223 59 62 3 849.33 6.89 224 59 63 4 831.55 2.00 225 60 63 3 863.91 3.39 226 60 64 4 845.30 4.88 227 60 158 98 409.43 5.22 228 61 64 3 845.93 4.56 229 61 65 4 845.49 4.78 230 61 68 7 828.85 2.91 231 61 122 61 560.31 1.93 232 61 137 76 476.96 2.46 233 61 139 78 476.52 2.49 234 61 142 81 475.08 2.25 235 61 143 82 466.56 0.32 236 61 152 91 411.37 4.87 237 61 153 92 410.42 5.25 238 61 154 93 410.00 5.07 239 62 65 3 853.02 5.58 240 62 66 4 848.47 3.29 241 62 139 77 476.51 2.48 242 63 66 3 848.47 3.29 243 63 67 4 839.50 1.20 244 64 67 3 839.50 1.20 245 64 68 4 839.50 1.20 246 64 119 55 560.32 1.93 247 64 154 90 409.44 5.23 248 64 156 92 409.39 5.24 249 64 158 94 409.18 5.24 250 65 68 3 839.52 1.21 251 65 69 4 826.14 7.89 252 65 136 71 476.75 2.45 253 65 137 72 476.70 2.46 254 65 139 74 476.51 2.48 255 66 69 3 839.53 1.20 256 66 70 4 837.02 2.46 257 67 70 3 839.53 1.20 258 67 71 4 837.04 2.46 259 67 119 52 560.24 1.97 260 68 71 3 852.66 4.94 261 68 72 4 834.12 3.94 262 68 119 51 560.23 1.97 263 68 122 54 560.15 1.96 264 68 133 65 477.04 2.44 265 68 136 68 476.69 2.47 266 68 137 69 476.69 2.47 267 69 72 3 848.35 7.10 268 69 73 4 834.12 3.94 269 69 136 67 476.69 2.47 270 70 73 3 843.12 0.57 271 70 74 4 828.10 1.54 272 71 74 3 828.10 1.54 273 71 101 30 611.19 0.82 274 71 102 31 611.16 0.83 275 71 106 35 609.89 1.00 276 71 119 48 560.21 1.97 277 72 106 34 609.88 0.99 278 72 119 47 560.23 1.97 279 72 120 48 560.13 1.95 280 72 133 61 476.90 2.44 281 72 135 63 476.69 2.46 282 72 136 64 476.69 2.47 283 74 103 29 609.90 0.99 284 74 106 32 608.52 1.56 285 75 103 28 609.34 1.06 286 76 80 4 810.00 7.53 287 76 103 27 599.86 1.80 288 76 106 30 563.82 0.70 289 76 107 31 561.33 1.85 290 77 80 3 836.64 2.29 291 77 81 4 816.39 4.00 292 78 81 3 842.40 3.39 293 78 82 4 816.38 4.00 294 78 230 152 102.74 7.57 295 78 234 156 92.12 2.23 296 79 82 3 843.49 2.85 297 79 83 4 836.81 6.21 298 79 229 150 97.62 0.43 299 79 230 151 92.11 2.25 300 79 233 154 92.12 2.23 301 80 83 3 856.38 3.56 302 80 84 4 845.43 1.90 303 80 103 23 573.79 4.20 304 80 104 24 563.56 0.74 305 80 107 27 560.78 1.88 306 81 84 3 845.43 1.91 307 81 85 4 845.46 1.88 308 81 107 26 560.71 1.91 309 82 85 3 845.47 1.89 310 82 86 4 845.46 1.88 311 82 233 151 92.10 2.23 312 82 234 152 92.00 2.26 313 82 237 155 88.34 0.78 314 83 86 3 850.11 9.36 315 83 87 4 832.39 4.66 316 83 92 9 675.29 15.71 317 83 95 12 611.22 0.82 318 83 182 99 313.15 1.41 319 83 184 101 312.99 1.42 320 83 233 150 92.06 2.24 321 84 87 3 843.60 2.44 322 84 88 4 832.40 4.65 323 84 107 23 560.69 1.91 324 84 108 24 560.58 1.90 325 84 109 25 560.42 1.96 326 85 88 3 833.34 4.17 327 85 89 4 832.38 4.63 328 85 237 152 87.52 1.19 329 85 241 156 79.33 1.09 330 86 89 3 850.61 5.96 331 86 90 4 832.33 4.61 332 86 91 5 767.93 4.78 333 86 92 6 706.91 0.09 334 86 182 96 313.10 1.42 335 86 233 147 92.06 2.24 336 86 237 151 87.17 1.03 337 86 240 154 78.95 1.06 338 87 90 3 836.79 5.86 339 87 92 5 712.63 2.77 340 87 109 22 560.41 1.96 341 87 110 23 560.36 1.94 342 87 111 24 560.33 1.95 343 89 237 148 87.19 1.02 344 89 241 152 76.76 1.09 345 89 244 155 63.26 3.83 346 90 93 3 913.67 1.78 347 91 240 149 77.81 1.59 348 91 244 153 59.97 5.48 349 92 95 3 931.63 0.49 350 93 111 18 560.27 1.94 351 94 111 17 560.27 1.94 352 94 113 19 560.16 1.96 353 94 174 80 313.33 1.43 354 95 104 9 561.13 1.88 355 95 109 14 560.45 1.95 356 95 111 16 560.31 1.94 357 95 112 17 560.19 1.95 358 95 113 18 560.16 1.96 359 96 113 17 560.16 1.96 360 96 115 19 560.14 1.96 361 96 171 75 313.56 1.38 362 96 174 78 313.52 1.41 363 96 175 79 313.49 1.41 364 96 196 100 213.41 1.62 365 97 104 7 563.51 0.76 366 97 112 15 560.36 1.96 367 97 113 16 560.28 1.94 368 97 114 17 560.15 1.96 369 97 115 18 560.14 1.96 370 98 115 17 560.15 1.96 371 98 116 18 559.99 1.89 372 98 186 88 312.34 1.56 373 98 193 95 213.79 1.64 374 99 114 15 560.34 1.96 375 100 116 16 560.27 1.94 376 100 117 17 560.15 1.95 377 100 118 18 553.09 0.34 378 100 119 19 545.05 1.11 379 100 156 56 388.77 1.92 380 101 114 13 560.49 1.95 381 102 105 3 844.44 5.04 382 102 106 4 833.49 2.14 383 102 120 18 521.06 0.95 384 103 106 3 834.04 1.86 385 103 107 4 820.04 4.58 386 104 107 3 849.75 1.41 387 104 108 4 833.48 2.14 388 104 109 5 751.39 1.78 389 104 112 8 627.88 0.50 390 105 108 3 834.28 4.03 391 105 109 4 787.99 6.44 392 105 112 7 662.10 4.17 393 105 114 9 627.88 0.50 394 109 112 3 897.61 2.37 395 113 167 54 313.64 1.41 396 113 170 57 313.55 1.38 397 113 171 58 313.50 1.40 398 113 174 61 313.49 1.41 399 114 117 3 849.90 7.33 400 114 118 4 835.84 0.29 401 114 167 53 313.70 1.43 402 115 157 42 384.84 0.00 403 115 163 48 314.17 1.30 404 115 167 52 313.72 1.43 405 115 168 53 313.70 1.43 406 115 171 56 313.64 1.41 407 115 193 78 213.81 1.61 408 115 196 81 213.79 1.62 409 116 156 40 388.63 1.84 410 116 157 41 384.19 0.32 411 116 163 47 314.38 1.22 412 116 190 74 234.61 10.59 413 116 193 77 213.88 1.60 414 117 155 38 394.72 1.05 415 117 156 39 388.63 1.84 416 117 157 40 388.63 1.84 417 118 121 3 838.98 1.65 418 118 155 37 401.39 4.36 419 118 156 38 398.69 3.02 420 119 156 37 401.98 4.07 421 120 132 12 477.04 2.44 422 120 133 13 476.95 2.46 423 120 134 14 476.95 2.46 424 121 130 9 477.28 2.52 425 121 131 10 477.21 2.50 426 121 132 11 477.04 2.44 427 121 154 33 409.46 5.24 428 121 155 34 409.06 5.22 429 122 130 8 477.28 2.52 430 122 131 9 477.24 2.52 431 122 133 11 477.19 2.52 432 122 153 31 409.53 5.23 433 122 154 32 409.47 5.24 434 122 155 33 409.31 5.26 435 123 129 6 477.31 2.51 436 123 130 7 477.28 2.51 437 123 152 29 410.05 5.07 438 123 153 30 409.57 5.25 439 123 155 32 409.48 5.24 440 124 127 3 855.62 3.24 441 124 129 5 477.32 2.50 442 124 145 21 411.55 4.83 443 124 148 24 411.73 4.88 444 124 150 26 411.46 4.88 445 124 151 27 411.19 4.90 446 124 152 28 410.07 5.07 447 125 151 26 411.37 4.84 448 125 153 28 410.10 5.07 449 126 148 22 411.75 4.88 450 126 150 24 411.54 4.85 451 127 145 18 428.24 1.16 452 127 147 20 411.78 4.88 453 127 148 21 411.76 4.88 454 129 147 18 411.76 4.88 455 131 152 21 410.12 5.08 456 137 143 6 669.63 28.65 457 139 142 3 932.48 0.64 458 139 143 4 892.57 0.43 459 142 151 9 411.55 4.87 460 143 151 8 411.56 4.87 461 143 152 9 411.48 4.88 462 144 150 6 411.73 4.88 463 144 151 7 411.63 4.91 464 144 152 8 411.51 4.88 465 145 148 3 868.16 0.22 466 145 149 4 678.03 29.49 467 145 150 5 411.75 4.88 468 145 152 7 411.48 4.89 469 153 158 5 879.58 0.77 470 153 161 8 780.56 12.10 471 154 158 4 917.15 6.01 472 157 161 4 796.40 4.17 473 157 163 6 314.40 1.22 474 157 164 7 314.44 1.24 475 158 161 3 842.25 2.60 476 163 168 5 809.64 11.40 477 163 192 29 214.08 1.51 478 164 167 3 852.53 0.76 479 164 168 4 832.41 0.10 480 165 168 3 851.57 3.33 481 165 169 4 814.12 9.25 482 166 169 3 837.68 0.53 483 166 170 4 838.53 3.19 484 167 170 3 844.26 2.76 485 167 171 4 825.36 9.77 486 168 171 3 843.25 2.25 487 168 172 4 830.90 7.01 488 168 192 24 213.87 1.57 489 168 196 28 213.66 1.56 490 169 172 3 841.40 1.77 491 169 173 4 839.87 2.52 492 170 173 3 847.25 5.46 493 170 174 4 838.10 0.89 494 171 174 3 839.63 1.65 495 171 175 4 837.37 1.25 496 171 196 25 213.41 1.62 497 172 175 3 873.75 9.15 498 172 176 4 841.61 0.88 499 172 196 24 213.41 1.62 500 172 199 27 211.92 1.05 501 173 176 3 862.82 3.68 502 173 177 4 846.63 1.64 503 174 177 3 865.08 2.56 504 174 178 4 835.28 1.46 505 175 178 3 854.20 8.00 506 175 181 6 649.14 13.56 507 175 196 21 213.40 1.62 508 175 199 24 211.49 1.27 509 175 200 25 211.38 1.31 510 176 199 23 211.48 1.27 511 176 203 27 210.78 1.48 512 178 200 22 211.31 1.28 513 179 205 26 210.63 1.47 514 179 206 27 210.63 1.47 515 180 200 20 211.50 1.38 516 180 206 26 210.53 1.53 517 180 208 28 210.50 1.52 518 181 197 16 213.16 1.61 519 181 200 19 211.53 1.38 520 181 206 25 210.74 1.48 521 181 207 26 210.52 1.52 522 181 208 27 210.50 1.52 523 182 208 26 210.48 1.52 524 182 210 28 210.42 1.51 525 182 233 51 92.06 2.24 526 182 236 54 89.72 2.30 527 183 186 3 909.87 4.29 528 183 197 14 213.25 1.61 529 183 207 24 210.76 1.48 530 183 208 25 210.63 1.50 531 183 209 26 210.44 1.51 532 183 210 27 210.44 1.51 533 183 217 34 156.50 0.83 534 184 210 26 210.41 1.51 535 185 210 25 210.47 1.53 536 185 211 26 210.41 1.51 537 186 211 25 210.42 1.51 538 186 215 29 170.56 0.13 539 187 215 28 167.88 0.51 540 187 216 29 167.87 0.51 541 188 194 6 419.53 104.55 542 188 215 27 172.66 6.33 543 188 216 28 163.41 1.73 544 188 217 29 163.23 1.64 545 189 194 5 832.28 0.82 546 189 216 27 163.43 1.72 547 190 193 3 904.89 0.99 548 190 194 4 852.61 2.93 549 191 194 3 870.37 4.42 550 191 195 4 856.96 2.29 551 192 195 3 860.96 0.29 552 192 196 4 856.97 2.29 553 193 196 3 871.21 6.02 554 193 197 4 881.06 1.09 555 194 197 3 891.54 3.46 556 195 198 3 857.87 0.65 557 195 199 4 853.54 2.81 558 196 199 3 863.12 0.12 559 196 200 4 855.00 3.83 560 197 200 3 860.85 0.91 561 197 201 4 846.01 0.07 562 197 207 10 210.75 1.49 563 197 217 20 156.46 0.84 564 198 201 3 851.23 2.69 565 198 202 4 831.24 1.26 566 198 217 19 156.45 0.84 567 198 220 22 156.03 0.74 568 199 202 3 846.68 6.18 569 199 203 4 831.13 1.30 570 200 203 3 851.08 3.98 571 200 204 4 829.61 1.17 572 200 205 5 756.95 11.01 573 200 206 6 758.23 10.37 574 201 204 3 835.76 1.91 575 201 205 4 800.62 3.73 576 201 207 6 730.38 2.27 577 201 220 19 156.02 0.73 578 201 222 21 155.86 0.74 579 206 222 16 155.85 0.74 580 207 217 10 156.47 0.83 581 207 220 13 156.03 0.74 582 207 221 14 155.92 0.76 583 207 222 15 155.86 0.73 584 207 223 16 155.85 0.73 585 208 223 15 155.76 0.78 586 208 225 17 155.52 0.86 587 209 215 6 437.61 135.43 588 209 217 8 157.38 0.39 589 209 218 9 156.43 0.80 590 209 221 12 156.03 0.73 591 209 223 14 155.88 0.73 592 209 224 15 155.52 0.86 593 209 225 16 155.50 0.87 594 209 226 17 155.46 0.90 595 210 225 15 155.44 0.89 596 210 227 17 155.29 0.83 597 210 229 19 92.97 1.88 598 210 232 22 92.11 2.22 599 210 233 23 92.11 2.23 600 210 236 26 92.00 2.25 601 211 215 4 862.34 3.56 602 211 225 14 155.85 0.73 603 211 226 15 155.44 0.89 604 211 227 16 155.33 0.85 605 212 226 14 155.61 0.85 606 212 227 15 155.41 0.88 607 213 226 13 155.94 0.72 608 214 218 4 830.32 6.02 609 214 226 12 155.94 0.72 610 215 218 3 849.59 0.60 611 215 219 4 876.43 1.92 612 216 219 3 900.35 3.27 613 217 220 3 878.11 3.12 614 217 221 4 839.38 2.56 615 218 221 3 846.67 0.48 616 218 224 6 399.35 120.95 617 218 226 8 155.97 0.73 618 221 224 3 857.56 0.55 619 225 232 7 378.36 140.90 620 227 232 5 788.19 6.07 621 228 231 3 862.44 4.57 622 228 232 4 806.59 6.20 623 229 232 3 847.51 2.90 624 229 233 4 795.87 2.23 625 230 234 4 809.51 4.75 626 231 234 3 845.86 2.09 627 231 235 4 828.86 1.62 628 232 235 3 848.24 4.08 629 232 236 4 828.85 1.62 630 233 236 3 830.53 0.88 631 233 237 4 828.85 1.62 632 234 237 3 849.94 6.25 633 234 238 4 820.45 0.64 634 235 238 3 835.12 1.19 635 235 239 4 820.46 0.64 636 236 239 3 841.03 1.87 637 236 240 4 820.44 0.63 638 237 240 3 835.60 4.59 639 237 241 4 820.72 0.78 640 238 241 3 838.28 6.04 641 238 242 4 820.75 0.81 642 239 242 3 836.36 7.00 643 239 243 4 827.79 2.71 644 240 243 3 832.58 2.25 645 240 244 4 831.03 1.09 646 241 244 3 837.95 3.48 647 241 245 4 824.53 2.16 648 242 245 3 833.05 1.60 649 242 246 4 824.53 2.16 650 243 246 3 863.52 1.40 651 243 247 4 863.52 1.40 652 244 247 3 856.83 1.96