BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 203 200 140.65 14.22 2 3 204 201 140.65 14.23 3 3 205 202 140.16 14.05 4 3 227 224 113.16 0.57 5 4 35 31 797.55 3.41 6 4 36 32 797.52 3.39 7 4 37 33 797.51 3.39 8 4 38 34 797.51 3.39 9 4 205 201 140.47 14.14 10 5 37 32 797.52 3.39 11 5 38 33 797.52 3.39 12 5 221 216 113.33 0.58 13 5 226 221 113.38 0.59 14 5 227 222 113.30 0.59 15 5 228 223 113.25 0.60 16 5 247 242 18.03 0.04 17 6 37 31 797.54 3.39 18 6 38 32 797.53 3.38 19 6 40 34 797.52 3.39 20 6 90 84 265.43 39.05 21 6 124 118 231.22 30.47 22 6 161 155 230.90 30.46 23 6 205 199 141.28 14.31 24 6 207 201 140.30 14.05 25 6 227 221 113.56 0.50 26 6 228 222 113.30 0.59 27 6 229 223 113.29 0.59 28 7 37 30 797.61 3.38 29 7 38 31 797.56 3.39 30 7 39 32 797.53 3.39 31 7 40 33 797.53 3.39 32 7 228 221 113.38 0.59 33 7 229 222 113.33 0.60 34 7 231 224 113.23 0.60 35 7 240 233 47.99 0.72 36 7 243 236 44.55 0.39 37 7 244 237 34.17 0.17 38 7 247 240 18.05 0.04 39 8 40 32 797.53 3.38 40 8 42 34 797.52 3.39 41 8 229 221 113.59 0.52 42 8 230 222 113.28 0.59 43 8 231 223 113.24 0.60 44 8 240 232 48.00 0.72 45 9 40 31 797.58 3.39 46 9 41 32 797.52 3.39 47 9 42 33 797.52 3.39 48 9 231 222 113.28 0.59 49 9 235 226 82.00 0.21 50 9 240 231 48.00 0.72 51 10 64 54 373.75 39.36 52 10 92 82 269.01 38.30 53 10 95 85 262.56 41.23 54 10 230 220 113.78 0.56 55 10 231 221 113.55 0.54 56 10 235 225 88.01 0.18 57 11 14 3 865.50 2.99 58 11 20 9 799.02 3.20 59 11 23 12 798.74 3.19 60 11 24 13 798.74 3.19 61 11 27 16 798.72 3.19 62 11 41 30 797.66 3.36 63 11 42 31 797.58 3.38 64 11 43 32 797.54 3.39 65 11 51 40 694.19 1.12 66 11 235 224 103.05 0.98 67 11 236 225 85.36 0.25 68 12 44 32 797.54 3.39 69 12 64 52 373.72 39.40 70 12 95 83 267.09 39.25 71 12 97 85 267.13 39.27 72 13 44 31 797.63 3.35 73 14 44 30 797.66 3.36 74 14 236 222 113.11 0.58 75 15 19 4 831.73 3.81 76 15 20 5 831.08 4.13 77 15 47 32 797.59 3.38 78 16 19 3 859.56 3.72 79 16 20 4 833.90 2.73 80 16 47 31 797.64 3.36 81 17 20 3 863.87 2.35 82 17 21 4 838.82 1.52 83 17 47 30 797.64 3.36 84 17 50 33 743.32 2.43 85 18 21 3 841.91 3.30 86 18 22 4 838.57 1.64 87 19 22 3 866.91 2.31 88 19 23 4 838.22 3.70 89 20 23 3 855.97 1.69 90 20 24 4 837.06 4.28 91 20 47 27 797.44 3.30 92 20 50 30 732.32 3.75 93 20 51 31 707.36 7.40 94 20 54 34 684.50 3.83 95 21 24 3 867.60 1.97 96 21 25 4 845.10 0.27 97 21 50 29 732.32 3.75 98 21 54 33 679.53 1.38 99 22 25 3 865.26 2.96 100 22 26 4 845.09 0.26 101 23 26 3 854.57 2.50 102 23 27 4 842.77 0.90 103 23 235 212 107.78 0.46 104 23 236 213 94.70 2.25 105 23 237 214 82.08 0.24 106 23 238 215 81.55 0.45 107 24 27 3 842.95 0.97 108 24 28 4 841.78 3.56 109 24 51 27 707.14 7.50 110 24 54 30 448.00 6.17 111 24 55 31 430.27 14.23 112 25 28 3 854.68 2.44 113 25 29 4 852.14 2.87 114 26 29 3 867.50 0.04 115 26 238 212 79.39 0.56 116 26 241 215 64.38 1.39 117 27 30 3 870.66 0.26 118 27 55 28 443.91 7.42 119 27 59 32 428.76 13.72 120 27 235 208 99.82 0.26 121 27 237 210 81.64 0.43 122 27 238 211 74.80 1.74 123 27 240 213 59.38 3.76 124 27 241 214 47.98 0.72 125 28 54 26 447.78 6.34 126 28 55 27 430.23 14.24 127 28 56 28 430.15 14.27 128 28 58 30 429.50 14.04 129 28 59 31 428.80 13.71 130 30 58 28 428.03 13.97 131 30 241 211 47.97 0.72 132 30 244 214 47.45 0.68 133 30 245 215 45.86 0.18 134 30 248 218 40.34 0.10 135 31 58 27 425.56 14.31 136 32 39 7 798.45 3.39 137 32 58 26 418.05 17.89 138 32 59 27 418.08 17.87 139 32 248 216 29.07 0.03 140 33 36 3 860.15 2.71 141 33 37 4 821.02 2.13 142 34 37 3 855.96 1.41 143 34 58 24 374.43 39.41 144 37 244 207 40.74 0.19 145 37 247 210 25.43 1.54 146 37 248 211 17.91 0.01 147 38 205 167 141.31 14.32 148 39 59 20 374.45 39.43 149 39 60 21 374.43 39.43 150 39 244 205 36.49 0.85 151 40 60 20 374.45 39.42 152 40 61 21 374.36 39.47 153 40 62 22 374.35 39.47 154 40 90 50 270.24 37.94 155 41 51 10 429.36 13.64 156 41 55 14 427.50 13.63 157 41 59 18 417.89 17.87 158 41 60 19 374.69 39.43 159 41 61 20 374.36 39.47 160 41 62 21 374.36 39.47 161 42 62 20 374.35 39.46 162 42 63 21 374.34 39.47 163 42 64 22 374.23 39.45 164 42 90 48 271.31 37.52 165 42 91 49 271.26 37.55 166 42 92 50 271.23 37.55 167 42 124 82 270.99 37.66 168 43 47 4 778.29 19.27 169 43 48 5 754.36 31.21 170 43 51 8 694.10 1.15 171 43 62 19 374.71 39.42 172 43 64 21 374.32 39.45 173 44 47 3 838.22 6.73 174 44 48 4 837.98 6.83 175 45 48 3 855.11 5.51 176 47 50 3 856.26 0.28 177 47 51 4 826.68 0.58 178 48 51 3 854.57 5.25 179 48 52 4 833.18 2.66 180 48 61 13 418.96 17.39 181 48 62 14 417.93 17.90 182 48 88 40 273.64 37.37 183 49 52 3 852.44 1.64 184 49 53 4 835.89 2.98 185 50 53 3 866.78 5.46 186 50 54 4 845.94 1.15 187 51 54 3 859.52 2.77 188 51 55 4 843.92 0.14 189 52 55 3 855.09 2.65 190 52 56 4 851.72 0.96 191 52 57 5 843.01 0.58 192 52 59 7 800.35 3.37 193 52 60 8 428.09 13.97 194 52 61 9 419.93 17.18 195 52 89 37 272.34 37.09 196 55 59 4 827.18 7.87 197 55 61 6 633.60 41.90 198 56 59 3 851.73 6.65 199 59 244 185 40.65 0.01 200 60 89 29 271.17 37.60 201 60 90 30 270.37 37.98 202 61 89 28 271.25 37.56 203 61 90 29 270.98 37.68 204 62 88 26 271.44 37.58 205 62 90 28 271.16 37.59 206 63 66 3 930.77 1.41 207 63 78 15 275.54 37.03 208 63 83 20 274.03 37.49 209 63 88 25 273.66 37.47 210 63 90 27 271.31 37.54 211 63 91 28 271.24 37.56 212 63 92 29 271.25 37.56 213 64 92 28 271.27 37.55 214 65 92 27 271.35 37.52 215 65 98 33 272.43 37.23 216 65 104 39 273.22 36.85 217 65 112 47 274.14 37.30 218 66 78 12 275.67 37.05 219 66 80 14 274.51 37.32 220 66 91 25 274.05 37.34 221 66 93 27 273.50 37.21 222 66 112 46 274.14 37.30 223 66 116 50 274.25 37.36 224 66 121 55 274.26 37.37 225 67 77 10 276.12 36.83 226 67 78 11 275.69 37.05 227 67 79 12 275.37 36.93 228 67 112 45 274.53 37.20 229 68 108 40 274.53 37.20 230 68 111 43 274.54 37.20 231 68 112 44 274.54 37.20 232 69 74 5 564.46 77.48 233 69 79 10 276.53 36.99 234 70 79 9 276.53 36.99 235 70 81 11 276.55 36.98 236 71 79 8 276.65 36.95 237 71 81 10 276.66 36.95 238 71 82 11 276.66 36.95 239 74 77 3 869.60 0.83 240 78 82 4 849.27 2.60 241 79 82 3 855.75 0.56 242 79 83 4 849.28 2.60 243 80 83 3 849.29 2.60 244 80 84 4 845.90 4.29 245 80 115 35 403.33 21.50 246 80 116 36 293.53 30.01 247 80 119 39 404.48 21.70 248 80 121 41 404.39 21.74 249 81 84 3 849.29 2.61 250 81 85 4 845.90 4.30 251 81 119 38 404.50 21.70 252 82 85 3 854.27 0.12 253 82 86 4 845.90 4.30 254 83 86 3 844.59 4.96 255 83 87 4 844.41 5.04 256 83 88 5 670.14 64.73 257 83 121 38 422.19 15.14 258 84 87 3 823.73 5.29 259 84 119 35 456.17 1.87 260 84 120 36 456.20 1.86 261 84 121 37 427.59 11.42 262 88 121 33 532.45 1.82 263 89 122 33 532.41 1.81 264 90 122 32 532.45 1.82 265 90 124 34 532.41 1.81 266 90 161 71 532.47 1.82 267 91 116 25 532.77 1.81 268 91 121 30 532.71 1.80 269 91 122 31 532.66 1.79 270 91 123 32 532.47 1.82 271 91 124 33 532.47 1.82 272 92 95 3 900.84 1.75 273 92 124 32 532.48 1.82 274 92 126 34 532.44 1.80 275 92 230 138 116.03 0.53 276 93 109 16 532.74 1.80 277 93 112 19 532.92 1.78 278 93 113 20 532.84 1.79 279 93 116 23 532.96 1.79 280 93 123 30 532.72 1.80 281 93 124 31 532.62 1.77 282 93 125 32 532.48 1.82 283 93 126 33 532.47 1.81 284 94 99 5 737.74 33.84 285 94 104 10 532.86 1.79 286 94 109 15 532.84 1.79 287 94 126 32 532.51 1.80 288 95 98 3 850.64 1.06 289 95 99 4 825.02 2.46 290 95 126 31 532.60 1.76 291 95 165 70 532.54 1.78 292 95 230 135 116.48 0.61 293 96 99 3 841.50 3.52 294 96 100 4 825.02 2.46 295 96 165 69 532.38 1.85 296 96 167 71 531.55 1.52 297 96 170 74 495.29 0.40 298 97 100 3 839.87 1.03 299 97 101 4 825.00 2.46 300 98 102 4 824.91 2.48 301 98 104 6 533.27 1.77 302 99 102 3 871.88 2.38 303 99 103 4 688.12 8.96 304 99 104 5 533.26 1.77 305 99 106 7 533.59 1.93 306 99 109 10 533.29 1.75 307 99 126 27 532.89 1.78 308 99 127 28 532.86 1.76 309 99 146 47 532.95 1.77 310 104 108 4 823.13 0.98 311 104 109 5 823.20 0.95 312 104 112 8 822.91 1.03 313 105 108 3 843.49 4.77 314 105 109 4 837.16 7.93 315 106 109 3 852.76 0.14 316 106 110 4 843.49 4.76 317 106 145 39 533.26 1.70 318 106 146 40 533.06 1.77 319 106 149 43 533.26 1.70 320 107 110 3 843.48 4.78 321 107 111 4 827.49 1.13 322 108 111 3 841.37 5.82 323 108 112 4 841.38 5.80 324 109 112 3 847.82 2.60 325 109 113 4 841.39 5.80 326 109 125 16 533.12 1.74 327 109 150 41 533.40 1.72 328 110 113 3 856.89 1.95 329 110 114 4 841.42 5.79 330 110 149 39 533.38 1.71 331 110 150 40 533.38 1.71 332 110 152 42 533.97 1.57 333 110 153 43 533.98 1.58 334 111 114 3 860.57 6.41 335 111 115 4 841.41 5.79 336 112 115 3 860.37 6.51 337 112 116 4 833.76 1.96 338 113 116 3 860.38 6.50 339 113 117 4 833.80 1.94 340 113 123 10 533.45 1.70 341 113 125 12 533.31 1.67 342 113 153 40 534.23 1.58 343 113 160 47 533.87 1.55 344 114 118 4 846.46 4.39 345 114 153 39 534.12 1.65 346 115 118 3 848.84 3.21 347 115 119 4 847.53 3.87 348 116 119 3 880.07 2.21 349 116 120 4 847.52 3.87 350 116 121 5 622.22 24.75 351 117 120 3 847.51 3.87 352 117 121 4 829.72 1.82 353 117 153 36 584.16 2.96 354 117 154 37 580.89 4.02 355 117 160 43 581.41 4.29 356 122 159 37 664.17 1.50 357 123 159 36 664.25 1.50 358 123 160 37 664.20 1.50 359 123 161 38 664.20 1.49 360 124 161 37 664.20 1.49 361 124 163 39 664.18 1.51 362 124 207 83 274.88 42.76 363 124 230 106 116.00 0.56 364 125 150 25 664.36 1.55 365 125 160 35 664.28 1.49 366 125 161 36 664.27 1.50 367 125 162 37 664.23 1.49 368 125 163 38 664.22 1.50 369 126 163 37 664.23 1.50 370 126 165 39 664.19 1.51 371 126 230 104 116.31 0.66 372 127 143 16 664.70 1.67 373 127 146 19 664.60 1.62 374 127 147 20 664.60 1.62 375 127 162 35 664.35 1.51 376 127 163 36 664.28 1.50 377 127 164 37 664.25 1.50 378 127 165 38 664.25 1.50 379 127 188 61 300.76 34.18 380 128 143 15 664.70 1.67 381 128 146 18 664.76 1.62 382 128 164 36 664.27 1.50 383 128 165 37 664.25 1.50 384 128 167 39 664.12 1.53 385 129 133 4 828.08 6.26 386 129 134 5 746.31 34.62 387 129 139 10 664.94 1.58 388 129 142 13 664.91 1.57 389 129 164 35 664.36 1.51 390 129 167 38 664.15 1.54 391 129 168 39 664.13 1.53 392 130 133 3 833.22 8.83 393 130 134 4 833.22 8.83 394 130 167 37 664.16 1.54 395 130 168 38 664.10 1.54 396 131 134 3 854.22 1.68 397 131 135 4 837.51 1.54 398 131 168 37 664.00 1.61 399 131 172 41 629.53 2.44 400 131 174 43 603.18 4.25 401 132 135 3 846.04 2.41 402 132 136 4 840.50 0.04 403 133 136 3 839.81 0.70 404 133 137 4 839.39 0.51 405 133 138 5 686.08 8.81 406 133 142 9 665.00 1.59 407 134 137 3 837.65 0.37 408 134 139 5 665.04 1.59 409 134 168 34 664.25 1.51 410 138 141 3 847.95 5.09 411 138 142 4 839.76 3.02 412 139 142 3 851.98 3.08 413 139 143 4 851.98 3.08 414 140 143 3 854.00 4.09 415 140 144 4 841.21 2.30 416 140 187 47 300.90 34.17 417 141 144 3 861.11 3.23 418 141 145 4 845.00 0.41 419 142 145 3 857.38 3.05 420 142 146 4 841.25 2.28 421 143 146 3 852.71 2.85 422 143 147 4 841.06 2.18 423 143 164 21 664.35 1.50 424 143 184 41 300.92 34.18 425 143 188 45 300.81 34.16 426 143 191 48 300.78 34.16 427 144 147 3 858.16 5.58 428 144 148 4 843.38 1.24 429 144 191 47 300.78 34.16 430 145 148 3 850.13 1.82 431 145 149 4 843.73 1.40 432 146 149 3 860.83 3.04 433 146 150 4 860.82 3.03 434 147 150 3 891.62 3.62 435 147 151 4 843.06 1.21 436 147 188 41 300.76 34.16 437 147 191 44 300.65 34.22 438 148 151 3 874.95 4.73 439 148 191 43 300.73 34.18 440 148 195 47 298.46 34.30 441 150 153 3 884.25 2.16 442 150 162 12 664.34 1.49 443 151 154 3 871.93 4.00 444 151 192 41 299.30 33.91 445 151 195 44 295.62 34.92 446 151 196 45 294.88 34.59 447 154 157 3 881.00 4.83 448 154 160 6 664.50 1.44 449 154 162 8 664.50 1.44 450 154 204 50 277.53 42.20 451 156 159 3 878.56 0.38 452 157 192 35 297.56 34.69 453 157 196 39 294.37 34.77 454 157 200 43 277.60 42.19 455 157 203 46 277.53 42.20 456 157 204 47 277.47 42.23 457 158 161 3 932.41 0.86 458 158 205 47 277.43 42.22 459 160 204 44 277.44 42.21 460 161 205 44 276.99 42.27 461 161 207 46 274.94 42.79 462 162 188 26 300.25 34.31 463 162 192 30 297.64 34.74 464 162 204 42 277.47 42.23 465 162 205 43 277.35 42.24 466 162 206 44 274.93 42.80 467 162 207 45 274.94 42.79 468 163 207 44 274.92 42.79 469 163 209 46 274.34 42.74 470 163 230 67 116.37 0.73 471 164 184 20 300.82 34.19 472 164 185 21 300.76 34.20 473 164 188 24 300.75 34.19 474 164 207 43 274.94 42.79 475 164 208 44 274.81 42.79 476 164 209 45 274.51 42.66 477 165 170 5 838.78 3.39 478 165 209 44 274.27 42.69 479 166 169 3 871.36 4.84 480 166 170 4 838.78 3.39 481 166 176 10 301.48 34.34 482 166 208 42 275.00 42.81 483 166 209 43 274.88 42.78 484 166 210 44 274.22 42.68 485 167 170 3 849.51 1.97 486 168 172 4 842.13 1.72 487 168 174 6 622.09 19.91 488 168 176 8 303.92 34.52 489 168 180 12 302.56 33.87 490 169 172 3 846.14 3.72 491 169 174 5 553.10 1.70 492 169 175 6 307.36 31.44 493 169 176 7 302.53 33.85 494 170 209 39 274.90 42.77 495 170 210 40 273.97 42.59 496 176 180 4 830.60 1.88 497 176 208 32 276.41 42.56 498 177 180 3 845.38 4.04 499 177 181 4 848.38 2.54 500 178 181 3 848.48 2.49 501 178 182 4 848.48 2.49 502 178 219 41 128.39 5.75 503 178 220 42 127.94 5.98 504 178 223 45 123.94 3.98 505 179 182 3 848.49 2.49 506 179 183 4 851.22 1.12 507 180 183 3 866.28 5.54 508 180 184 4 853.41 0.02 509 181 184 3 862.25 3.32 510 181 185 4 843.94 0.22 511 181 208 27 277.00 42.41 512 181 220 39 129.09 6.60 513 181 226 45 118.11 1.28 514 182 185 3 841.16 1.61 515 182 186 4 839.51 2.44 516 182 223 41 118.41 1.44 517 182 224 42 118.41 1.43 518 182 225 43 118.25 1.36 519 183 186 3 851.54 2.39 520 183 187 4 839.45 2.48 521 184 187 3 845.41 5.45 522 184 188 4 842.29 0.95 523 185 188 3 850.37 4.70 524 185 189 4 842.28 0.94 525 185 206 21 277.56 42.29 526 185 207 22 277.56 42.29 527 185 208 23 277.42 42.24 528 185 225 40 117.98 1.31 529 185 226 41 117.88 1.27 530 186 189 3 851.73 5.54 531 186 190 4 842.99 1.50 532 186 225 39 117.98 1.32 533 187 190 3 842.13 1.92 534 187 191 4 843.31 1.33 535 188 191 3 847.84 0.93 536 188 192 4 836.54 2.05 537 188 206 18 277.57 42.24 538 189 192 3 848.09 0.67 539 189 193 4 831.22 0.10 540 189 201 12 279.02 42.70 541 189 202 13 279.00 42.68 542 189 204 15 278.02 42.34 543 189 206 17 277.66 42.24 544 189 225 36 117.95 1.30 545 189 227 38 117.88 1.27 546 190 193 3 854.69 2.67 547 190 194 4 829.14 1.36 548 191 194 3 846.59 5.38 549 191 195 4 838.38 1.28 550 192 195 3 864.10 4.71 551 192 196 4 832.66 4.14 552 192 200 8 309.50 32.34 553 192 201 9 279.08 42.68 554 192 204 12 278.41 42.34 555 193 196 3 846.59 5.38 556 193 197 4 426.04 19.35 557 193 198 5 337.15 29.94 558 193 201 8 279.13 42.70 559 196 200 4 835.83 2.54 560 197 200 3 857.63 1.51 561 197 201 4 835.84 2.54 562 198 201 3 856.08 1.33 563 198 202 4 837.96 1.48 564 199 202 3 860.41 0.83 565 199 203 4 837.96 1.48 566 200 203 3 860.41 0.83 567 200 204 4 835.78 2.60 568 201 204 3 854.35 3.86 569 204 227 23 117.19 0.94 570 205 227 22 116.78 0.90 571 206 226 20 117.31 0.98 572 206 227 21 117.00 0.83 573 207 228 21 116.88 0.81 574 207 229 22 116.66 0.86 575 207 230 23 116.62 0.85 576 208 212 4 855.86 2.94 577 208 220 12 118.40 1.39 578 208 228 20 116.98 0.81 579 208 229 21 116.71 0.84 580 209 229 20 116.88 0.76 581 209 230 21 116.67 0.83 582 211 216 5 828.52 3.20 583 212 216 4 828.52 3.20 584 212 217 5 684.68 7.08 585 212 220 8 334.64 46.30 586 212 229 17 117.12 0.73 587 212 231 19 116.89 0.75 588 212 234 22 116.43 0.77 589 212 243 31 47.98 0.69 590 213 216 3 865.96 1.93 591 213 217 4 828.51 3.20 592 213 234 21 116.52 0.82 593 213 243 30 47.98 0.69 594 214 217 3 881.78 4.29 595 214 239 25 49.83 0.07 596 214 243 29 47.98 0.70 597 216 219 3 849.42 9.96 598 216 220 4 846.38 1.78 599 217 220 3 865.75 4.02 600 217 221 4 843.70 0.44 601 217 229 12 118.28 1.07 602 217 243 26 46.05 0.30 603 217 246 29 35.80 0.78 604 218 221 3 857.09 3.55 605 218 222 4 843.70 0.44 606 218 246 28 35.79 0.78 607 219 222 3 872.26 3.25 608 219 223 4 847.28 1.35 609 220 223 3 847.28 1.35 610 220 226 6 127.81 5.76 611 220 229 9 118.55 1.20 612 221 226 5 168.21 23.58 613 221 227 6 118.65 1.18 614 221 228 7 118.58 1.19 615 221 229 8 118.58 1.19 616 221 246 25 34.16 0.17 617 221 247 26 30.87 0.90 618 223 226 3 837.27 3.35 619 229 243 14 45.08 0.55 620 229 246 17 38.00 0.09 621 231 234 3 897.16 0.87 622 231 235 4 837.18 1.95 623 231 240 9 53.59 2.08 624 231 243 12 47.29 0.74 625 232 235 3 869.82 4.26 626 232 236 4 859.28 1.01 627 233 236 3 859.28 1.01 628 234 237 3 855.77 2.75 629 234 240 6 92.69 21.50 630 234 243 9 85.09 16.62 631 235 240 5 171.80 24.09 632 237 240 3 875.07 1.18 633 238 241 3 886.25 1.57 634 239 242 3 875.08 1.18 635 239 243 4 836.92 3.58 636 240 243 3 851.78 1.28 637 240 244 4 836.88 3.60 638 241 244 3 845.78 0.85 639 241 245 4 836.24 2.04 640 242 245 3 841.20 4.50 641 242 246 4 841.18 4.50 642 243 246 3 849.72 0.24 643 243 247 4 841.19 4.50 644 244 247 3 855.93 2.04 645 244 248 4 841.19 4.50 646 245 248 3 850.67 2.17