BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 114 112 23.99 0.26 2 2 136 134 24.31 0.26 3 2 137 135 24.42 0.32 4 2 141 139 24.73 0.42 5 3 134 131 24.86 0.38 6 3 135 132 24.87 0.39 7 3 136 133 24.84 0.38 8 3 137 134 24.85 0.38 9 3 138 135 25.10 0.31 10 3 168 165 25.12 0.32 11 5 9 4 809.70 2.86 12 5 10 5 540.64 10.47 13 5 13 8 527.13 16.89 14 5 136 131 33.82 0.07 15 6 9 3 863.21 1.05 16 6 10 4 725.06 45.69 17 7 10 3 855.46 2.83 18 7 11 4 825.15 0.65 19 8 11 3 825.03 0.71 20 8 12 4 820.59 2.93 21 8 57 49 46.59 0.55 22 8 88 80 46.90 0.52 23 8 112 104 47.59 0.28 24 8 184 176 47.01 0.56 25 8 185 177 47.01 0.56 26 8 187 179 46.99 0.56 27 9 12 3 834.95 2.86 28 9 13 4 815.58 0.42 29 9 112 103 47.29 0.43 30 9 135 126 47.61 0.28 31 9 136 127 47.61 0.28 32 9 184 175 47.01 0.56 33 9 185 176 47.02 0.56 34 10 13 3 850.04 0.02 35 10 14 4 824.08 3.85 36 11 14 3 837.60 1.54 37 11 15 4 824.92 4.79 38 11 87 76 53.32 0.27 39 11 88 77 52.84 0.20 40 12 15 3 837.60 1.54 41 12 16 4 825.96 4.28 42 12 85 73 52.77 0.22 43 12 88 76 52.85 0.20 44 12 112 100 52.88 0.16 45 13 16 3 830.98 2.77 46 13 17 4 825.96 4.28 47 13 114 101 58.33 0.03 48 13 136 123 56.26 1.05 49 14 17 3 838.65 1.07 50 14 18 4 837.63 1.57 51 15 18 3 837.78 1.51 52 15 19 4 810.12 2.52 53 15 87 72 59.67 0.31 54 15 88 73 59.67 0.30 55 16 19 3 815.21 5.71 56 16 112 96 64.11 1.52 57 16 114 98 65.38 0.98 58 16 115 99 67.02 1.76 59 16 118 102 65.45 0.97 60 17 114 97 65.42 0.97 61 17 115 98 74.89 2.19 62 18 115 97 79.56 0.68 63 19 84 65 78.79 0.41 64 19 86 67 78.78 0.41 65 19 93 74 79.83 0.53 66 19 111 92 78.20 1.22 67 19 115 96 81.43 1.29 68 20 93 73 81.60 1.14 69 21 117 96 81.80 1.17 70 21 121 100 81.78 1.18 71 22 93 71 81.78 1.18 72 22 97 75 86.83 2.12 73 22 121 99 81.86 1.15 74 22 124 102 90.61 0.24 75 22 125 103 86.17 2.44 76 24 97 73 99.06 1.66 77 24 124 100 99.89 1.72 78 26 30 4 881.09 0.23 79 26 123 97 125.61 3.20 80 26 126 100 113.83 0.36 81 26 127 101 125.78 3.29 82 26 128 102 135.66 0.46 83 27 101 74 125.28 3.81 84 28 100 72 134.05 1.27 85 28 103 75 134.35 1.35 86 28 126 98 139.25 1.09 87 28 128 100 152.83 0.03 88 30 123 93 159.22 0.76 89 30 128 98 155.83 1.40 90 30 152 122 160.71 0.97 91 30 153 123 160.72 0.97 92 30 154 124 160.68 0.96 93 31 104 73 159.19 0.74 94 31 105 74 159.79 1.04 95 31 106 75 159.12 0.70 96 31 128 97 160.45 1.06 97 32 35 3 828.82 0.26 98 32 36 4 819.78 2.38 99 32 106 74 160.58 1.01 100 33 36 3 824.08 2.11 101 33 37 4 819.78 2.38 102 33 49 16 160.66 0.97 103 33 78 45 160.66 0.96 104 33 79 46 160.58 1.01 105 33 106 73 160.65 0.97 106 33 108 75 160.66 0.97 107 33 129 96 160.69 0.96 108 33 176 143 167.90 1.01 109 34 37 3 820.00 2.48 110 34 38 4 819.79 2.37 111 34 155 121 168.06 1.09 112 34 176 142 167.64 0.89 113 34 206 172 168.38 0.78 114 35 38 3 822.45 0.00 115 35 39 4 815.90 0.42 116 36 39 3 823.98 2.94 117 36 40 4 808.98 1.57 118 36 77 41 169.32 0.53 119 36 78 42 169.30 0.53 120 36 105 69 169.21 0.57 121 36 106 70 169.19 0.56 122 37 40 3 823.73 0.66 123 37 41 4 811.76 6.65 124 37 47 10 169.33 0.54 125 37 49 12 169.33 0.54 126 37 78 41 169.32 0.54 127 37 176 139 171.12 0.98 128 37 206 169 171.10 0.99 129 37 208 171 169.44 0.51 130 37 228 191 169.44 0.51 131 37 229 192 169.41 0.49 132 38 41 3 811.77 6.65 133 38 42 4 811.77 6.65 134 38 206 168 171.05 1.02 135 38 228 190 170.98 1.02 136 39 42 3 836.53 0.84 137 39 43 4 820.08 2.50 138 40 43 3 826.01 6.10 139 40 44 4 811.69 6.61 140 40 45 5 647.20 72.33 141 40 47 7 169.44 0.51 142 40 78 38 169.44 0.51 143 41 44 3 827.92 1.51 144 41 223 182 171.19 0.99 145 41 228 187 171.13 0.98 146 46 219 173 227.25 0.33 147 46 230 184 228.13 0.50 148 47 78 31 489.86 2.50 149 47 228 181 228.40 0.48 150 47 229 182 228.14 0.50 151 47 230 183 228.14 0.50 152 48 77 29 490.14 2.52 153 48 78 30 489.94 2.50 154 48 79 31 489.94 2.50 155 48 219 171 228.17 0.49 156 48 230 182 228.15 0.50 157 48 232 184 228.12 0.49 158 48 236 188 210.12 0.36 159 48 243 195 125.98 0.77 160 48 246 198 113.45 0.34 161 48 247 199 106.66 0.30 162 49 78 29 490.14 2.52 163 49 79 30 489.94 2.50 164 49 80 31 489.91 2.48 165 49 81 32 489.89 2.48 166 49 208 159 229.52 0.24 167 49 229 180 229.47 0.23 168 49 230 181 228.31 0.54 169 49 231 182 228.16 0.50 170 49 232 183 228.15 0.49 171 50 73 23 490.38 2.46 172 50 77 27 490.34 2.46 173 50 79 29 490.27 2.48 174 50 80 30 489.98 2.51 175 50 81 31 489.98 2.51 176 50 232 182 228.41 0.45 177 50 236 186 212.15 1.13 178 51 69 18 491.11 2.55 179 51 81 30 490.01 2.53 180 51 83 32 490.05 2.54 181 51 110 59 489.97 2.52 182 51 231 180 229.47 0.23 183 51 232 181 228.81 0.30 184 51 233 182 228.06 0.47 185 51 237 186 210.15 0.37 186 52 64 12 491.57 2.65 187 52 65 13 491.55 2.65 188 52 66 14 490.51 2.40 189 52 69 17 491.12 2.56 190 52 81 29 490.28 2.48 191 52 82 30 490.12 2.54 192 52 83 31 490.11 2.54 193 52 95 43 490.24 2.48 194 52 237 185 211.08 0.10 195 53 56 3 909.30 1.40 196 53 60 7 492.75 2.88 197 53 83 30 490.12 2.54 198 53 233 180 229.44 0.23 199 53 234 181 229.08 0.27 200 53 237 184 221.91 0.73 201 54 61 7 492.57 2.85 202 54 62 8 492.18 2.75 203 54 66 12 491.60 2.65 204 54 90 36 492.28 3.18 205 54 95 41 491.75 2.83 206 55 60 5 492.73 2.88 207 55 61 6 492.69 2.89 208 55 87 32 492.91 3.02 209 55 88 33 492.86 2.99 210 55 89 34 492.44 3.12 211 55 90 35 492.49 3.09 212 56 59 3 836.29 1.05 213 56 60 4 492.84 2.92 214 56 88 32 492.92 3.02 215 56 89 33 492.84 3.00 216 57 88 31 492.97 3.03 217 57 89 32 492.95 3.02 218 57 187 130 231.08 0.48 219 58 187 129 231.07 0.48 220 60 234 174 231.01 0.47 221 60 237 177 229.18 0.33 222 60 238 178 228.41 0.43 223 62 237 175 228.22 0.34 224 62 238 176 228.19 0.35 225 64 69 5 768.34 16.04 226 64 237 173 228.02 0.42 227 64 238 174 227.80 0.30 228 64 240 176 223.36 1.55 229 65 68 3 843.73 9.29 230 65 69 4 823.41 1.06 231 66 69 3 832.74 3.79 232 66 70 4 823.43 1.06 233 66 95 29 495.47 3.83 234 66 99 33 495.48 3.77 235 67 70 3 833.54 3.37 236 67 71 4 826.65 2.68 237 67 98 31 495.53 3.79 238 67 99 32 495.50 3.78 239 67 102 35 495.38 3.57 240 68 71 3 830.36 4.96 241 68 72 4 817.80 3.87 242 69 72 3 831.19 4.55 243 69 73 4 823.75 0.83 244 69 236 167 227.72 0.34 245 69 237 168 227.19 0.21 246 69 240 171 150.79 11.77 247 70 73 3 827.33 1.24 248 70 74 4 827.38 0.98 249 70 80 10 495.44 3.77 250 70 99 29 495.63 3.75 251 70 103 33 495.68 3.65 252 71 74 3 838.08 0.12 253 71 75 4 838.22 0.04 254 71 102 31 495.80 3.66 255 71 103 32 495.84 3.64 256 72 75 3 849.87 0.72 257 72 76 4 844.44 1.71 258 72 240 168 154.75 13.75 259 72 241 169 127.62 0.20 260 72 243 171 126.10 0.70 261 72 244 172 126.06 0.71 262 73 76 3 853.35 5.55 263 73 77 4 823.79 0.82 264 73 80 7 495.84 3.73 265 73 236 163 227.06 0.15 266 73 240 167 127.77 0.26 267 73 243 170 125.98 0.76 268 74 103 29 530.16 13.52 269 74 104 30 538.01 17.44 270 74 105 31 530.10 13.54 271 76 243 167 125.98 0.76 272 76 244 168 126.06 0.71 273 76 247 171 108.53 0.39 274 77 105 28 550.91 23.86 275 78 106 28 550.90 23.86 276 79 105 26 550.95 23.87 277 79 106 27 550.87 23.88 278 80 99 19 551.23 23.89 279 80 103 23 551.09 23.92 280 80 105 25 551.05 23.92 281 80 106 26 550.99 23.94 282 80 107 27 547.85 25.39 283 80 108 28 547.78 25.41 284 81 108 27 547.70 25.46 285 81 110 29 547.53 25.50 286 81 231 150 229.42 0.21 287 82 95 13 551.46 23.86 288 82 96 14 551.38 23.82 289 82 99 17 551.38 23.83 290 82 107 25 547.97 25.45 291 82 108 26 547.82 25.52 292 82 109 27 547.52 25.54 293 82 110 28 547.46 25.56 294 83 89 6 552.12 23.71 295 83 91 8 551.78 23.79 296 83 110 27 547.19 25.70 297 84 91 7 551.78 23.79 298 84 92 8 551.76 23.79 299 84 93 9 551.76 23.79 300 84 96 12 551.68 23.75 301 84 109 25 547.40 25.73 302 84 110 26 547.25 25.73 303 84 111 27 547.21 25.73 304 85 88 3 874.15 0.06 305 85 89 4 570.91 14.36 306 85 90 5 552.18 23.72 307 85 91 6 552.08 23.70 308 85 111 26 547.20 25.73 309 85 112 27 547.10 25.79 310 86 90 4 552.18 23.72 311 86 91 5 552.15 23.72 312 86 92 6 552.10 23.71 313 89 112 23 547.30 25.77 314 92 95 3 834.44 2.48 315 92 96 4 822.58 0.64 316 93 96 3 832.71 3.33 317 93 97 4 816.47 2.09 318 93 109 16 547.82 25.55 319 93 121 28 243.74 1.51 320 93 125 32 243.74 1.44 321 94 97 3 829.47 0.55 322 94 98 4 820.34 4.02 323 95 98 3 839.44 1.78 324 95 99 4 816.17 2.23 325 96 99 3 815.46 0.82 326 96 100 4 809.18 0.53 327 96 107 11 548.07 25.43 328 96 109 13 547.66 25.61 329 96 125 29 243.65 1.49 330 96 127 31 243.42 1.48 331 97 100 3 822.49 2.95 332 97 101 4 812.05 0.90 333 97 125 28 243.63 1.50 334 98 101 3 834.44 1.34 335 98 102 4 811.36 0.56 336 99 102 3 827.86 4.63 337 99 103 4 811.36 0.56 338 99 107 8 550.42 24.25 339 100 103 3 852.21 3.92 340 100 107 7 550.78 24.05 341 100 125 25 243.66 1.51 342 100 126 26 243.41 1.54 343 103 107 4 868.44 1.63 344 106 128 22 242.34 1.98 345 107 127 20 242.72 1.82 346 107 128 21 242.38 1.98 347 107 129 22 242.35 1.98 348 108 129 21 242.35 1.97 349 108 130 22 242.34 1.98 350 108 131 23 242.31 1.98 351 108 157 49 239.71 0.70 352 108 231 123 229.40 0.21 353 109 113 4 889.60 2.45 354 109 118 9 244.28 1.57 355 109 122 13 243.60 1.45 356 109 127 18 243.43 1.48 357 109 129 20 242.84 1.76 358 109 130 21 242.34 1.98 359 109 131 22 242.46 1.91 360 110 131 21 242.47 1.89 361 110 210 100 238.03 0.03 362 110 231 121 236.63 0.39 363 112 118 6 758.47 25.32 364 112 133 21 242.82 1.82 365 112 136 24 243.00 2.14 366 112 184 72 241.95 1.52 367 113 118 5 566.09 14.05 368 113 119 6 649.64 26.44 369 113 130 17 243.89 1.71 370 113 131 18 243.50 1.53 371 113 132 19 243.00 1.75 372 113 137 24 243.43 1.94 373 113 145 32 244.79 1.46 374 114 118 4 836.14 3.13 375 114 136 22 243.41 1.95 376 114 137 23 243.41 1.95 377 114 145 31 244.85 1.43 378 115 118 3 856.45 3.38 379 115 145 30 245.02 1.38 380 116 119 3 837.55 2.43 381 116 120 4 823.81 2.08 382 116 145 29 245.17 1.32 383 116 148 32 246.43 1.93 384 117 120 3 835.19 3.60 385 117 121 4 823.80 2.08 386 118 121 3 823.73 2.11 387 118 122 4 823.81 2.07 388 119 122 3 823.81 2.06 389 119 123 4 823.80 2.07 390 119 130 11 245.52 1.21 391 119 145 26 264.23 3.95 392 119 148 29 265.83 3.15 393 119 149 30 264.25 3.90 394 119 152 33 274.76 1.33 395 120 123 3 827.98 2.98 396 120 124 4 814.56 2.55 397 120 148 28 274.82 1.34 398 120 152 32 274.82 1.34 399 121 124 3 830.21 0.29 400 121 125 4 816.50 1.58 401 122 125 3 830.20 0.29 402 122 126 4 816.39 1.53 403 122 127 5 664.27 33.94 404 122 130 8 263.99 4.02 405 122 154 32 274.64 1.26 406 123 126 3 830.20 0.29 407 123 152 29 278.41 0.34 408 123 154 31 274.79 1.32 409 127 154 27 291.50 5.39 410 128 154 26 291.48 5.40 411 129 154 25 291.48 5.40 412 129 155 26 292.03 5.13 413 129 156 27 292.02 5.12 414 130 156 26 292.97 4.67 415 130 157 27 292.44 4.93 416 131 157 26 293.43 4.90 417 131 159 28 294.67 4.62 418 132 142 10 296.73 4.44 419 132 145 13 296.72 4.44 420 132 146 14 296.66 4.42 421 132 156 24 294.73 4.62 422 132 157 25 294.69 4.62 423 132 158 26 294.69 4.62 424 132 159 27 295.55 4.20 425 133 136 3 849.68 6.89 426 133 137 4 812.78 1.54 427 133 159 26 295.92 4.32 428 133 184 51 296.94 4.40 429 134 137 3 831.98 1.05 430 134 142 8 302.91 7.18 431 134 158 24 296.68 4.42 432 134 159 25 296.65 4.44 433 134 160 26 296.77 4.38 434 134 165 31 297.19 4.41 435 134 168 34 297.41 4.43 436 134 169 35 297.29 4.46 437 135 161 26 297.19 4.42 438 135 165 30 297.38 4.42 439 135 183 48 297.17 4.41 440 135 184 49 297.18 4.41 441 137 141 4 817.67 7.81 442 137 142 5 795.10 3.48 443 137 145 8 756.18 22.78 444 138 141 3 827.93 2.69 445 138 142 4 818.46 7.42 446 139 142 3 832.87 1.08 447 139 143 4 819.99 6.66 448 139 168 29 301.55 5.66 449 139 171 32 302.04 5.74 450 139 172 33 301.79 5.62 451 140 143 3 840.72 2.85 452 140 144 4 829.98 1.66 453 141 144 3 830.09 1.70 454 141 145 4 820.12 3.29 455 142 145 3 840.64 2.88 456 142 146 4 820.12 3.29 457 142 158 16 301.33 5.56 458 142 168 26 302.28 5.65 459 142 172 30 302.29 5.66 460 143 146 3 828.43 0.44 461 143 147 4 823.75 1.47 462 143 172 29 302.24 5.67 463 144 147 3 830.75 2.10 464 144 148 4 823.75 1.47 465 145 148 3 836.28 0.67 466 145 149 4 814.88 3.00 467 146 149 3 836.28 0.67 468 146 150 4 823.25 7.18 469 146 156 10 302.06 5.51 470 146 158 12 302.33 5.62 471 146 172 26 543.11 18.97 472 147 150 3 826.80 5.44 473 147 151 4 823.70 1.43 474 148 151 3 829.02 1.23 475 148 152 4 824.58 0.99 476 149 152 3 843.34 0.24 477 149 153 4 824.58 0.99 478 149 154 5 801.37 0.74 479 149 156 7 674.33 25.48 480 150 153 3 814.75 5.92 481 150 154 4 801.38 0.73 482 150 155 5 801.37 0.74 483 150 156 6 767.40 10.27 484 150 173 23 714.22 16.38 485 150 175 25 700.11 23.42 486 155 175 20 751.20 23.61 487 155 176 21 751.17 23.61 488 156 176 20 751.26 23.57 489 157 176 19 751.26 23.57 490 157 177 20 751.25 23.57 491 157 178 21 751.16 23.59 492 157 208 51 702.01 0.94 493 157 231 74 394.44 2.62 494 158 169 11 751.54 23.49 495 158 172 14 751.62 23.49 496 158 174 16 751.42 23.55 497 158 176 18 751.39 23.54 498 158 177 19 751.27 23.56 499 158 178 20 751.26 23.56 500 159 178 19 751.27 23.56 501 159 180 21 751.33 23.52 502 159 184 25 724.41 9.99 503 159 210 51 702.12 0.99 504 160 165 5 751.68 23.54 505 160 166 6 751.70 23.55 506 160 169 9 751.72 23.50 507 160 177 17 751.44 23.54 508 160 178 18 751.40 23.53 509 160 179 19 751.38 23.52 510 160 180 20 751.37 23.51 511 160 197 37 703.06 0.76 512 160 201 41 702.76 0.93 513 161 165 4 818.00 5.24 514 161 180 19 751.38 23.52 515 161 182 21 751.39 23.49 516 161 183 22 744.72 20.14 517 161 193 32 703.02 0.70 518 161 197 36 703.08 0.76 519 162 165 3 836.89 1.03 520 162 166 4 808.30 0.40 521 162 197 35 703.13 0.79 522 163 166 3 825.69 1.19 523 163 167 4 811.01 1.43 524 163 196 33 703.14 0.80 525 163 197 34 703.14 0.80 526 163 200 37 702.94 0.92 527 164 167 3 822.62 4.36 528 164 168 4 811.58 1.70 529 165 168 3 829.86 2.02 530 165 169 4 811.58 1.71 531 166 169 3 822.89 5.99 532 166 170 4 825.34 7.21 533 166 177 11 751.70 23.44 534 166 197 31 703.13 0.81 535 166 200 34 702.92 0.96 536 166 201 35 702.88 0.98 537 167 170 3 843.21 1.71 538 167 171 4 825.36 7.21 539 168 171 3 843.20 1.72 540 168 172 4 833.56 3.10 541 169 172 3 850.06 5.15 542 169 173 4 831.47 4.14 543 169 174 5 792.76 2.93 544 169 177 8 751.72 23.43 545 170 173 3 825.15 2.05 546 170 177 7 751.86 23.39 547 170 202 32 702.83 1.19 548 174 177 3 883.86 1.31 549 175 205 30 737.08 18.36 550 175 206 31 737.14 18.39 551 176 206 30 737.07 18.43 552 176 208 32 737.06 18.43 553 177 201 24 737.29 18.42 554 177 205 28 737.25 18.41 555 177 206 29 737.20 18.41 556 177 207 30 737.06 18.44 557 177 208 31 737.06 18.44 558 178 208 30 737.07 18.44 559 178 210 32 737.02 18.45 560 179 194 15 737.93 18.19 561 179 197 18 737.93 18.19 562 179 198 19 737.66 18.32 563 179 201 22 737.56 18.37 564 179 207 28 737.25 18.41 565 179 208 29 737.19 18.42 566 179 209 30 737.04 18.45 567 179 210 31 737.03 18.45 568 180 184 4 854.78 1.50 569 180 210 30 737.05 18.45 570 180 211 31 737.03 18.45 571 181 188 7 738.43 18.26 572 181 189 8 738.13 18.23 573 181 190 9 738.07 18.21 574 181 194 13 737.93 18.21 575 181 210 29 737.17 18.43 576 181 211 30 737.06 18.45 577 182 187 5 738.45 18.25 578 182 188 6 738.43 18.26 579 182 189 7 738.14 18.22 580 182 211 29 737.06 18.44 581 183 186 3 851.12 2.97 582 183 187 4 738.47 18.25 583 183 188 5 738.46 18.25 584 184 187 3 836.31 5.97 585 189 211 22 737.18 18.43 586 189 212 23 737.12 18.41 587 190 193 3 842.80 7.79 588 190 194 4 828.46 1.08 589 191 194 3 828.47 1.07 590 191 195 4 828.46 1.08 591 191 212 21 737.22 18.41 592 191 218 27 553.51 22.78 593 192 195 3 828.77 1.24 594 192 196 4 819.79 3.25 595 193 196 3 842.56 7.91 596 193 197 4 818.62 2.67 597 194 197 3 828.94 1.73 598 194 198 4 816.32 3.82 599 194 209 15 737.27 18.41 600 194 210 16 737.25 18.41 601 195 198 3 835.79 2.25 602 195 199 4 816.32 3.81 603 195 221 26 396.15 2.73 604 195 222 27 396.07 2.71 605 195 224 29 396.10 2.72 606 195 225 30 395.99 2.77 607 196 199 3 839.94 0.18 608 196 200 4 817.00 3.48 609 197 200 3 830.74 4.41 610 197 201 4 820.42 0.74 611 198 201 3 837.44 4.69 612 198 207 9 737.35 18.44 613 198 222 24 395.72 2.68 614 198 225 27 395.58 2.75 615 198 227 29 395.21 2.68 616 201 205 4 839.09 1.24 617 201 207 6 738.71 18.16 618 202 205 3 857.86 3.93 619 202 207 5 827.49 1.82 620 203 207 4 861.72 3.50 621 206 228 22 395.13 2.69 622 207 226 19 395.46 2.72 623 207 227 20 395.20 2.69 624 207 228 21 395.17 2.68 625 207 229 22 395.10 2.67 626 208 227 19 395.31 2.74 627 208 229 21 394.92 2.68 628 208 230 22 394.89 2.67 629 208 231 23 394.86 2.68 630 209 213 4 850.79 0.10 631 209 222 13 395.44 2.73 632 209 227 18 395.44 2.73 633 209 229 20 395.17 2.68 634 209 230 21 394.87 2.68 635 209 231 22 394.81 2.70 636 210 231 21 394.74 2.73 637 211 231 20 394.82 2.71 638 213 218 5 746.33 18.29 639 213 219 6 742.49 16.37 640 213 222 9 742.73 16.25 641 213 230 17 395.18 2.69 642 213 232 19 394.81 2.70 643 213 235 22 394.69 2.69 644 214 235 21 394.69 2.69 645 214 242 28 317.30 6.84 646 214 246 32 126.34 0.76 647 215 218 3 837.72 0.18 648 215 219 4 831.12 3.48 649 215 242 27 317.86 6.62 650 215 246 31 126.17 0.68 651 216 219 3 831.38 3.35 652 216 220 4 816.73 0.60 653 216 245 29 126.34 0.76 654 216 246 30 126.07 0.69 655 216 248 32 126.06 0.69 656 217 220 3 844.30 3.48 657 217 221 4 816.73 0.60 658 218 221 3 833.38 1.99 659 218 222 4 823.38 2.75 660 219 222 3 828.44 3.87 661 219 223 4 823.35 2.77 662 219 230 11 395.84 2.75 663 219 232 13 395.69 2.69 664 219 246 27 125.66 0.86 665 220 223 3 827.20 1.33 666 220 224 4 826.51 1.20 667 221 224 3 836.16 0.01 668 221 225 4 826.52 1.20 669 222 225 3 830.83 2.68 670 222 226 4 826.51 1.20 671 222 227 5 750.53 20.14 672 222 230 8 478.66 37.35 673 223 226 3 829.56 0.93 674 223 227 4 754.94 18.12 675 223 228 5 754.10 18.36 676 223 230 7 596.96 96.50 677 227 230 3 956.25 1.82 678 232 235 3 884.29 2.58 679 232 236 4 830.24 2.91 680 232 246 14 124.25 0.17 681 233 236 3 837.93 0.94 682 233 237 4 823.97 1.87 683 234 237 3 833.25 1.40 684 234 238 4 817.13 1.54 685 235 238 3 828.14 3.96 686 235 239 4 816.49 1.85 687 235 246 11 398.28 2.08 688 236 239 3 830.20 2.88 689 236 240 4 752.15 18.03 690 236 242 6 593.50 94.83 691 236 243 7 591.10 94.33 692 236 246 10 358.81 21.82 693 237 240 3 827.34 6.72 694 239 242 3 851.31 1.74 695 240 243 3 861.30 3.26 696 241 244 3 845.57 4.59 697 242 245 3 828.02 1.88 698 242 246 4 818.05 3.11 699 243 246 3 818.97 2.66 700 243 247 4 812.94 3.39 701 244 247 3 828.75 0.41 702 244 248 4 813.58 0.04 703 245 248 3 836.57 4.42