BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 114 112 27.03 1.11 2 2 136 134 27.24 1.09 3 2 137 135 27.23 1.09 4 2 138 136 27.60 1.04 5 2 141 139 27.59 1.04 6 3 134 131 27.61 1.05 7 3 135 132 27.61 1.05 8 3 136 133 27.59 1.05 9 3 137 134 27.60 1.04 10 3 138 135 27.61 1.05 11 3 168 165 27.78 1.11 12 5 9 4 810.59 2.90 13 5 10 5 784.78 1.98 14 5 13 8 608.35 89.98 15 5 136 131 34.32 0.32 16 6 9 3 863.71 0.80 17 6 10 4 810.56 2.90 18 7 10 3 850.85 5.63 19 7 11 4 825.32 0.73 20 8 11 3 824.49 1.47 21 8 12 4 817.82 0.27 22 8 57 49 48.80 0.07 23 8 88 80 48.87 0.06 24 8 89 81 48.88 0.05 25 8 112 104 48.88 0.05 26 8 184 176 48.84 0.04 27 8 185 177 48.84 0.04 28 8 187 179 48.84 0.04 29 9 12 3 835.45 2.61 30 9 13 4 817.80 0.26 31 9 112 103 48.88 0.04 32 9 135 126 48.88 0.04 33 9 136 127 48.88 0.04 34 9 184 175 48.85 0.04 35 9 185 176 48.84 0.04 36 10 13 3 829.40 1.74 37 10 14 4 815.61 0.84 38 11 14 3 828.99 5.85 39 11 15 4 831.22 6.96 40 11 87 76 55.54 0.00 41 11 88 77 55.37 0.02 42 12 15 3 836.46 2.12 43 12 16 4 822.88 8.90 44 12 85 73 54.87 0.23 45 12 88 76 55.47 0.03 46 12 112 100 54.12 0.62 47 13 16 3 839.10 0.80 48 13 17 4 811.05 2.99 49 13 114 101 61.00 0.11 50 13 136 123 57.02 0.70 51 14 17 3 849.65 4.42 52 14 18 4 822.88 8.95 53 15 18 3 838.28 1.26 54 15 19 4 809.98 2.58 55 15 85 70 65.53 0.80 56 15 87 72 65.59 0.78 57 15 88 73 64.75 0.46 58 16 19 3 810.16 2.50 59 16 112 96 65.63 0.75 60 16 114 98 66.54 0.83 61 16 115 99 68.27 1.53 62 16 118 102 67.77 1.30 63 17 114 97 68.16 1.48 64 17 115 98 69.27 2.04 65 18 115 97 71.34 2.66 66 19 84 65 70.06 2.37 67 19 85 66 70.05 2.37 68 19 86 67 70.05 2.36 69 19 93 74 78.16 1.33 70 19 111 92 70.09 2.35 71 19 115 96 80.36 1.75 72 21 117 96 80.47 1.75 73 21 121 100 80.47 1.73 74 22 93 71 80.53 1.76 75 22 97 75 81.23 1.60 76 22 121 99 80.51 1.72 77 22 124 102 85.02 3.52 78 22 125 103 82.31 2.48 79 24 97 73 98.08 1.93 80 24 124 100 105.40 4.33 81 26 29 3 950.20 1.49 82 26 30 4 899.19 2.83 83 26 123 97 136.16 0.21 84 26 124 98 136.11 0.21 85 26 126 100 131.31 0.59 86 26 127 101 136.21 0.19 87 26 128 102 137.75 0.65 88 28 100 72 136.64 0.24 89 28 103 75 138.98 1.42 90 28 107 79 143.97 0.64 91 28 126 98 138.07 0.87 92 28 128 100 145.23 0.01 93 30 123 93 158.66 0.48 94 30 128 98 149.52 2.12 95 30 152 122 159.45 0.43 96 30 153 123 159.44 0.43 97 30 154 124 159.42 0.44 98 31 104 73 158.66 0.48 99 31 106 75 158.66 0.48 100 31 128 97 159.26 0.47 101 32 35 3 827.94 0.18 102 32 36 4 820.47 2.24 103 32 106 74 159.40 0.42 104 33 36 3 833.40 2.92 105 33 37 4 816.38 0.19 106 33 49 16 159.34 0.46 107 33 78 45 159.42 0.43 108 33 79 46 159.33 0.46 109 33 106 73 159.42 0.43 110 33 108 75 159.43 0.43 111 33 129 96 159.47 0.44 112 33 176 143 168.14 1.14 113 34 37 3 830.82 3.10 114 34 38 4 819.23 1.61 115 34 155 121 168.19 1.16 116 34 176 142 167.50 0.83 117 34 206 172 169.53 0.70 118 35 38 3 829.73 3.64 119 35 39 4 814.08 1.34 120 36 39 3 829.73 3.64 121 36 40 4 811.76 0.18 122 36 77 41 169.90 0.74 123 36 78 42 169.88 0.73 124 36 105 69 169.88 0.73 125 36 106 70 169.84 0.73 126 37 40 3 824.24 0.40 127 37 41 4 805.40 3.37 128 37 47 10 169.91 0.73 129 37 49 12 169.91 0.73 130 37 78 41 169.91 0.73 131 37 176 139 170.24 0.65 132 37 206 169 170.22 0.64 133 37 208 171 169.96 0.74 134 37 228 191 169.96 0.74 135 37 229 192 169.93 0.73 136 38 41 3 815.74 0.41 137 38 42 4 805.10 3.21 138 38 206 168 170.23 0.62 139 39 42 3 826.51 5.85 140 39 43 4 799.51 0.42 141 40 43 3 826.51 5.85 142 40 44 4 799.19 0.58 143 40 45 5 752.14 1.48 144 40 47 7 169.95 0.75 145 40 78 38 169.96 0.74 146 41 44 3 808.53 1.95 147 41 223 182 171.03 1.02 148 41 228 187 171.03 1.02 149 46 219 173 227.00 0.17 150 46 230 184 227.53 0.24 151 46 246 200 116.02 0.47 152 46 247 201 108.20 0.23 153 47 78 31 750.72 1.33 154 47 228 181 228.18 0.38 155 47 229 182 227.56 0.24 156 47 230 183 227.56 0.24 157 48 77 29 750.77 1.32 158 48 78 30 750.74 1.33 159 48 79 31 750.72 1.33 160 48 219 171 227.56 0.24 161 48 230 182 227.56 0.24 162 48 232 184 227.52 0.24 163 48 236 188 210.75 0.01 164 48 243 195 124.45 1.51 165 48 246 198 115.61 0.29 166 48 247 199 108.11 0.27 167 49 78 29 750.77 1.33 168 49 79 30 750.72 1.33 169 49 80 31 750.72 1.33 170 49 81 32 750.72 1.33 171 49 208 159 285.94 1.27 172 49 229 180 229.54 0.06 173 49 230 181 227.83 0.14 174 49 231 182 227.57 0.24 175 49 232 183 227.59 0.23 176 50 73 23 750.87 1.33 177 50 77 27 750.82 1.32 178 50 79 29 750.80 1.33 179 50 80 30 750.74 1.34 180 50 81 31 750.73 1.34 181 50 232 182 227.81 0.14 182 50 236 186 212.61 0.91 183 51 69 18 751.13 1.35 184 51 81 30 750.74 1.34 185 51 83 32 750.73 1.33 186 51 110 59 620.71 23.46 187 51 231 180 229.58 0.06 188 51 232 181 228.54 0.14 189 51 233 182 227.53 0.24 190 51 237 186 210.77 0.01 191 52 64 12 751.29 1.31 192 52 66 14 751.23 1.30 193 52 69 17 751.21 1.32 194 52 81 29 750.79 1.32 195 52 82 30 750.75 1.32 196 52 83 31 750.74 1.32 197 52 95 43 623.00 23.09 198 52 237 185 212.59 0.91 199 53 56 3 904.10 0.96 200 53 60 7 751.58 1.30 201 53 83 30 750.74 1.32 202 53 89 36 629.27 22.45 203 53 233 180 229.56 0.06 204 53 234 181 229.51 0.07 205 53 237 184 221.68 1.61 206 54 61 7 751.54 1.30 207 54 62 8 751.39 1.28 208 54 66 12 751.32 1.31 209 54 90 36 629.27 22.45 210 54 95 41 624.12 22.75 211 54 237 183 226.83 0.09 212 55 60 5 751.59 1.30 213 55 61 6 751.55 1.30 214 55 88 33 732.41 1.16 215 55 89 34 631.11 21.76 216 55 90 35 629.52 22.55 217 56 59 3 817.22 8.48 218 56 60 4 751.60 1.30 219 56 88 32 737.75 3.83 220 56 89 33 706.64 4.28 221 57 88 31 737.78 3.83 222 57 89 32 737.78 3.83 223 57 187 130 317.35 7.06 224 58 182 124 326.85 11.81 225 58 187 129 317.32 7.04 226 60 234 174 229.71 0.04 227 60 237 177 229.49 0.06 228 60 238 178 228.27 0.32 229 62 237 175 227.79 0.09 230 62 238 176 227.63 0.07 231 64 69 5 789.96 3.30 232 64 237 173 227.60 0.04 233 64 238 174 227.36 0.16 234 64 240 176 221.08 1.33 235 65 68 3 840.65 6.73 236 65 69 4 802.35 9.48 237 66 69 3 829.08 1.97 238 66 70 4 811.92 4.69 239 66 95 29 623.03 23.12 240 66 99 33 622.90 23.07 241 67 70 3 829.90 1.55 242 67 71 4 827.15 2.93 243 67 98 31 622.91 23.07 244 67 99 32 622.90 23.08 245 67 102 35 622.52 23.09 246 68 71 3 836.21 2.54 247 68 72 4 818.25 0.93 248 69 72 3 829.88 5.71 249 69 73 4 824.25 1.08 250 69 236 167 227.61 0.04 251 69 237 168 226.40 0.18 252 69 240 171 152.25 15.00 253 70 73 3 830.12 0.34 254 70 74 4 827.97 0.73 255 70 80 10 751.01 1.26 256 70 99 29 622.62 23.19 257 70 103 33 621.50 23.54 258 71 74 3 838.58 0.14 259 71 75 4 836.35 3.44 260 71 102 31 621.77 23.45 261 71 103 32 621.50 23.54 262 72 75 3 842.22 0.52 263 72 76 4 840.33 1.47 264 72 240 168 145.37 11.94 265 72 241 169 124.53 1.53 266 72 243 171 124.48 1.51 267 72 244 172 124.47 1.51 268 73 76 3 840.43 1.42 269 73 77 4 818.76 3.85 270 73 80 7 751.07 1.28 271 73 236 163 226.50 0.10 272 73 240 167 124.55 1.53 273 73 243 170 124.45 1.50 274 74 103 29 621.48 23.55 275 74 104 30 621.47 23.54 276 74 105 31 621.26 23.55 277 76 243 167 124.45 1.52 278 76 244 168 124.42 1.50 279 76 247 171 109.03 0.14 280 77 105 28 621.19 23.56 281 78 106 28 621.14 23.55 282 79 105 26 621.18 23.56 283 79 106 27 621.12 23.55 284 80 99 19 622.50 23.12 285 80 103 23 621.49 23.54 286 80 105 25 621.28 23.54 287 80 106 26 621.18 23.54 288 80 107 27 621.04 23.54 289 80 108 28 621.04 23.54 290 81 108 27 620.91 23.54 291 81 110 29 620.74 23.48 292 81 231 150 229.55 0.06 293 82 95 13 622.93 23.06 294 82 96 14 622.83 23.05 295 82 99 17 622.83 23.05 296 82 107 25 621.22 23.52 297 82 108 26 621.16 23.54 298 82 109 27 620.87 23.52 299 82 110 28 620.75 23.47 300 83 89 6 629.27 22.46 301 83 91 8 625.84 21.91 302 83 110 27 620.74 23.48 303 84 91 7 627.24 21.46 304 84 92 8 625.85 21.91 305 84 93 9 623.28 23.18 306 84 96 12 623.24 23.18 307 84 109 25 621.12 23.48 308 84 110 26 620.95 23.55 309 84 111 27 620.77 23.49 310 85 88 3 868.83 2.60 311 85 89 4 764.74 0.72 312 85 90 5 680.55 3.15 313 85 91 6 629.26 22.46 314 85 111 26 620.75 23.49 315 85 112 27 620.75 23.49 316 86 90 4 680.54 3.15 317 86 91 5 629.34 22.46 318 86 92 6 629.32 22.47 319 89 112 23 620.74 23.49 320 92 95 3 834.94 2.23 321 92 96 4 818.38 2.73 322 93 96 3 830.72 4.33 323 93 97 4 808.15 1.86 324 93 109 16 621.32 23.46 325 93 121 28 442.18 68.28 326 93 125 32 441.70 68.44 327 94 97 3 829.49 0.56 328 94 98 4 807.30 2.31 329 95 98 3 829.97 0.80 330 95 99 4 807.30 2.31 331 96 99 3 836.82 3.09 332 96 100 4 804.83 0.76 333 96 107 11 621.45 23.53 334 96 109 13 621.24 23.50 335 96 125 29 441.98 68.28 336 96 127 31 441.80 68.26 337 97 100 3 808.38 2.48 338 97 101 4 806.22 1.45 339 97 125 28 441.73 68.41 340 98 101 3 841.20 1.53 341 98 102 4 808.94 0.09 342 99 102 3 821.53 1.01 343 99 103 4 808.94 0.09 344 99 107 8 621.46 23.53 345 100 107 7 621.47 23.53 346 100 125 25 442.04 68.25 347 100 126 26 441.67 68.36 348 103 107 4 854.02 5.28 349 106 128 22 441.24 68.18 350 107 127 20 441.36 68.19 351 107 128 21 441.29 68.20 352 107 129 22 441.30 68.19 353 108 129 21 441.29 68.20 354 108 130 22 441.23 68.17 355 108 131 23 441.21 68.17 356 108 157 49 396.53 45.86 357 108 231 123 229.56 0.06 358 109 113 4 893.61 0.95 359 109 118 9 442.50 68.11 360 109 122 13 441.82 68.15 361 109 127 18 441.80 68.19 362 109 129 20 441.38 68.19 363 109 130 21 441.26 68.16 364 109 131 22 441.26 68.16 365 110 131 21 441.25 68.14 366 110 210 100 299.78 1.38 367 110 231 121 232.53 1.02 368 111 131 20 441.49 68.05 369 112 118 6 807.62 2.93 370 112 133 21 441.76 67.89 371 112 136 24 434.81 64.27 372 112 184 72 327.56 10.87 373 113 118 5 784.52 8.62 374 113 119 6 762.62 2.35 375 113 130 17 442.26 68.00 376 113 131 18 442.25 67.99 377 113 132 19 441.92 67.88 378 113 137 24 437.38 65.48 379 113 145 32 399.19 46.33 380 114 118 4 836.64 2.88 381 114 136 22 441.92 67.73 382 114 137 23 433.90 63.73 383 114 145 31 399.46 46.23 384 115 118 3 839.20 2.31 385 115 145 30 399.53 46.19 386 116 119 3 837.45 2.48 387 116 120 4 818.22 0.57 388 116 145 29 399.73 46.09 389 116 148 32 406.69 42.52 390 117 120 3 819.50 0.07 391 117 121 4 818.22 0.57 392 118 121 3 820.81 0.58 393 118 122 4 816.64 1.08 394 119 122 3 816.65 1.08 395 119 123 4 816.64 1.08 396 119 130 11 450.22 64.09 397 119 132 13 488.84 44.77 398 119 145 26 446.97 22.39 399 119 148 29 447.12 22.22 400 119 149 30 446.83 22.32 401 119 152 33 447.44 21.61 402 119 154 35 446.65 21.91 403 120 123 3 824.57 3.04 404 120 124 4 815.49 0.51 405 120 148 28 447.96 21.89 406 120 152 32 470.11 10.27 407 121 124 3 826.88 6.19 408 121 125 4 807.80 0.90 409 122 125 3 830.70 0.04 410 122 126 4 811.87 1.18 411 122 127 5 524.54 37.43 412 122 130 8 489.79 44.38 413 122 154 32 535.74 22.66 414 123 126 3 822.64 4.07 415 123 152 29 535.75 22.64 416 123 154 31 535.73 22.64 417 127 154 27 578.58 44.12 418 128 154 26 578.56 44.13 419 129 154 25 578.56 44.14 420 129 155 26 578.56 44.13 421 129 156 27 577.64 44.56 422 130 149 19 578.81 44.11 423 130 154 24 578.71 44.16 424 130 156 26 577.66 44.57 425 130 157 27 577.54 44.62 426 131 157 26 577.54 44.62 427 131 159 28 577.51 44.63 428 132 142 10 579.47 43.85 429 132 145 13 579.45 43.85 430 132 146 14 579.35 43.84 431 132 156 24 578.63 44.20 432 132 157 25 578.29 44.32 433 132 158 26 577.53 44.62 434 132 159 27 577.52 44.63 435 133 136 3 838.52 0.81 436 133 137 4 814.88 0.45 437 133 159 26 577.66 44.56 438 133 184 51 372.31 10.90 439 134 137 3 829.27 4.21 440 134 142 8 579.62 43.83 441 134 158 24 578.31 44.34 442 134 159 25 577.99 44.47 443 134 160 26 577.76 44.51 444 134 165 31 456.66 21.97 445 134 168 34 453.70 23.41 446 134 169 35 453.69 23.41 447 135 161 26 577.88 44.46 448 135 165 30 466.71 17.71 449 135 183 48 430.60 21.95 450 135 184 49 399.82 24.55 451 137 141 4 809.09 2.57 452 137 142 5 777.29 11.31 453 137 145 8 627.74 20.60 454 138 141 3 828.41 2.94 455 138 142 4 816.94 6.49 456 139 142 3 833.37 1.33 457 139 143 4 826.23 1.84 458 139 168 29 464.56 17.98 459 139 171 32 453.82 23.35 460 139 172 33 453.54 23.34 461 140 143 3 841.22 2.60 462 140 144 4 826.23 1.84 463 141 144 3 838.16 4.12 464 141 145 4 811.53 1.34 465 142 145 3 841.14 2.63 466 142 146 4 811.82 1.50 467 142 158 16 578.66 44.19 468 142 168 26 456.60 21.95 469 142 172 30 452.74 23.43 470 143 146 3 823.70 1.93 471 143 147 4 811.87 1.50 472 143 172 29 452.63 23.47 473 144 147 3 823.19 2.17 474 144 148 4 811.85 1.52 475 145 148 3 827.31 5.66 476 145 149 4 815.40 0.26 477 146 149 3 836.77 0.92 478 146 150 4 827.30 5.66 479 146 156 10 578.84 44.21 480 146 158 12 578.73 44.17 481 146 172 26 452.62 23.47 482 147 150 3 819.76 1.89 483 147 151 4 815.50 0.24 484 148 151 3 822.54 0.51 485 148 152 4 825.06 0.76 486 149 152 3 842.30 0.76 487 149 153 4 815.50 0.24 488 149 154 5 782.06 10.90 489 149 156 7 583.78 46.70 490 150 153 3 809.25 2.88 491 150 154 4 790.00 6.92 492 150 155 5 782.06 10.89 493 150 156 6 762.20 0.96 494 150 173 23 452.44 23.43 495 150 175 25 451.10 23.91 496 155 175 20 451.10 23.91 497 155 176 21 450.98 23.92 498 156 176 20 451.00 23.93 499 157 176 19 450.99 23.94 500 157 177 20 450.95 23.93 501 157 178 21 450.93 23.93 502 157 208 51 348.58 21.05 503 157 231 74 232.40 0.96 504 158 169 11 452.99 23.62 505 158 172 14 452.59 23.48 506 158 174 16 451.12 23.91 507 158 176 18 451.09 23.91 508 158 177 19 450.95 23.93 509 158 178 20 450.94 23.93 510 159 178 19 450.92 23.94 511 159 180 21 450.91 23.94 512 159 184 25 404.73 22.11 513 159 210 51 347.82 21.22 514 160 165 5 501.29 0.35 515 160 166 6 453.84 23.38 516 160 169 9 453.81 23.36 517 160 177 17 451.10 23.92 518 160 178 18 451.05 23.91 519 160 179 19 450.94 23.93 520 160 180 20 450.92 23.94 521 160 197 37 361.41 15.81 522 160 201 41 359.43 16.11 523 161 165 4 810.59 2.18 524 161 180 19 450.93 23.94 525 161 182 21 450.32 24.19 526 161 183 22 437.56 25.43 527 161 193 32 361.87 15.71 528 161 197 36 361.75 15.68 529 162 165 3 819.88 4.10 530 162 166 4 812.07 1.44 531 162 197 35 361.82 15.69 532 163 166 3 819.34 4.37 533 163 167 4 808.36 0.42 534 163 196 33 361.87 15.71 535 163 197 34 361.85 15.70 536 163 200 37 361.26 15.85 537 164 167 3 824.48 7.63 538 164 168 4 812.05 1.44 539 165 168 3 824.80 5.03 540 165 169 4 822.56 6.15 541 166 169 3 822.57 6.15 542 166 170 4 822.58 6.13 543 166 177 11 451.22 23.93 544 166 197 31 361.70 15.68 545 166 200 34 360.60 15.69 546 166 201 35 359.47 16.10 547 167 170 3 837.48 1.31 548 167 171 4 822.58 6.13 549 168 171 3 823.03 2.66 550 168 172 4 810.28 0.01 551 169 172 3 847.84 6.75 552 169 173 4 813.27 1.49 553 169 174 5 716.77 24.31 554 169 177 8 451.12 23.91 555 170 173 3 820.27 1.96 556 170 177 7 505.44 3.12 557 170 202 32 359.13 16.12 558 174 177 3 882.98 1.76 559 174 205 31 348.84 21.07 560 175 205 30 348.80 21.08 561 175 206 31 348.79 21.07 562 176 205 29 348.81 21.09 563 176 206 30 348.76 21.07 564 176 208 32 348.68 21.08 565 177 201 24 358.92 16.23 566 177 205 28 348.82 21.09 567 177 206 29 348.78 21.08 568 177 207 30 348.69 21.09 569 177 208 31 348.67 21.08 570 178 208 30 348.60 21.07 571 178 210 32 347.82 21.22 572 179 194 15 361.10 15.89 573 179 197 18 360.12 16.38 574 179 198 19 360.96 15.85 575 179 201 22 359.28 16.19 576 179 207 28 348.77 21.09 577 179 208 29 348.70 21.11 578 179 209 30 348.39 21.16 579 179 210 31 347.94 21.29 580 180 184 4 845.50 3.64 581 180 210 30 347.36 21.46 582 180 211 31 347.36 21.46 583 181 189 8 362.16 15.72 584 181 190 9 362.02 15.73 585 181 191 10 361.84 15.72 586 181 194 13 361.37 15.83 587 181 210 29 347.56 21.56 588 181 211 30 347.48 21.52 589 182 187 5 362.80 15.63 590 182 188 6 362.76 15.63 591 182 189 7 362.22 15.69 592 182 211 29 347.31 21.46 593 183 186 3 839.73 2.00 594 183 187 4 364.10 14.99 595 183 188 5 362.76 15.63 596 184 187 3 836.81 5.72 597 189 212 23 347.19 21.54 598 190 193 3 838.12 5.42 599 190 194 4 809.50 2.03 600 191 194 3 823.50 1.89 601 191 195 4 819.37 0.17 602 191 212 21 347.94 21.46 603 191 218 27 320.65 15.54 604 192 195 3 823.10 1.69 605 192 196 4 816.17 1.44 606 193 196 3 823.11 1.69 607 193 197 4 816.17 1.44 608 194 197 3 829.11 1.31 609 194 198 4 814.54 2.23 610 194 209 15 348.72 21.12 611 194 210 16 348.72 21.12 612 195 198 3 833.03 4.13 613 195 199 4 818.11 2.40 614 195 222 27 241.69 3.22 615 195 225 30 233.38 0.93 616 196 199 3 840.44 0.43 617 196 200 4 818.12 2.39 618 197 200 3 831.24 4.17 619 197 201 4 815.75 3.57 620 198 201 3 842.60 0.64 621 198 207 9 348.88 21.13 622 198 222 24 233.32 0.93 623 198 225 27 233.21 0.98 624 198 227 29 233.14 0.98 625 201 205 4 821.09 4.48 626 201 207 6 348.94 21.10 627 202 205 3 842.38 2.85 628 202 207 5 821.08 4.48 629 203 207 4 852.68 0.40 630 206 228 22 232.73 1.00 631 207 226 19 233.17 0.99 632 207 227 20 232.90 0.94 633 207 228 21 232.87 0.94 634 207 229 22 232.74 1.00 635 208 227 19 233.08 1.00 636 208 229 21 232.74 1.00 637 208 230 22 232.72 1.00 638 208 231 23 232.72 1.01 639 209 213 4 849.87 0.85 640 209 222 13 233.16 0.97 641 209 227 18 233.18 0.99 642 209 229 20 233.05 0.99 643 209 230 21 232.74 0.99 644 209 231 22 232.72 1.01 645 210 231 21 232.74 0.99 646 211 231 20 233.03 1.00 647 213 218 5 681.49 50.04 648 213 219 6 637.46 69.83 649 213 222 9 402.57 4.10 650 213 230 17 233.25 0.96 651 213 232 19 233.04 0.99 652 213 235 22 232.69 1.02 653 214 235 21 232.62 0.98 654 214 242 28 126.73 0.93 655 214 246 32 124.52 1.50 656 215 218 3 835.12 1.48 657 215 219 4 829.00 4.54 658 215 246 31 124.50 1.51 659 216 219 3 831.88 3.10 660 216 220 4 828.99 4.54 661 216 245 29 124.49 1.52 662 216 246 30 124.39 1.53 663 216 248 32 124.36 1.51 664 217 220 3 844.80 3.23 665 217 221 4 820.34 2.41 666 218 221 3 834.11 2.12 667 218 222 4 815.39 1.71 668 219 222 3 816.84 2.43 669 219 223 4 815.39 1.71 670 219 230 11 233.54 0.93 671 219 232 13 233.53 0.92 672 219 246 27 124.17 1.44 673 220 223 3 818.60 3.29 674 220 224 4 818.61 3.29 675 221 224 3 833.52 1.81 676 221 225 4 818.62 3.29 677 222 225 3 827.53 0.53 678 222 226 4 818.61 3.29 679 222 227 5 799.16 3.36 680 222 230 8 284.48 26.38 681 223 226 3 830.06 0.68 682 223 227 4 799.38 3.25 683 223 228 5 799.38 3.25 684 223 230 7 779.48 5.73 685 227 230 3 931.64 0.82 686 232 235 3 882.12 3.66 687 232 236 4 818.74 3.34 688 232 246 14 122.92 0.83 689 233 236 3 833.75 1.15 690 233 237 4 803.79 8.72 691 234 237 3 842.12 5.34 692 234 238 4 803.78 8.71 693 235 238 3 828.59 3.69 694 235 239 4 803.75 8.72 695 235 246 11 124.82 1.34 696 236 239 3 825.15 2.00 697 236 240 4 605.88 35.73 698 236 242 6 363.81 15.65 699 236 243 7 178.74 28.62 700 236 246 10 124.42 1.47 701 239 242 3 839.64 4.09 702 240 243 3 852.79 7.52 703 241 244 3 846.07 4.84 704 242 245 3 828.52 1.62 705 242 246 4 827.13 0.93 706 243 246 3 827.13 0.93 707 243 247 4 827.13 0.93 708 244 247 3 844.44 3.29 709 244 248 4 824.86 2.06 710 245 248 3 837.17 4.61