BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 697 702 5 763.87 1.53 2 697 703 6 582.47 88.91 3 698 703 5 764.32 2.00 4 699 703 4 827.69 0.49 5 700 703 3 865.39 2.10 6 701 815 114 97.86 1.11 7 701 816 115 88.87 0.85 8 701 817 116 88.71 0.94 9 702 705 3 860.09 0.70 10 702 817 115 95.64 2.12 11 703 707 4 900.19 2.41 12 704 707 3 876.65 0.61 13 704 708 4 866.41 3.86 14 704 716 12 107.42 0.06 15 705 708 3 876.65 0.61 16 705 716 11 247.75 69.91 17 705 798 93 107.30 0.10 18 705 817 112 107.48 0.01 19 705 818 113 107.30 0.10 20 705 819 114 106.82 0.04 21 705 821 116 106.98 0.12 22 706 709 3 867.91 3.10 23 706 937 231 63.98 0.65 24 707 711 4 848.72 0.69 25 708 711 3 847.37 0.39 26 708 713 5 788.02 14.46 27 708 714 6 760.75 0.82 28 708 715 7 760.74 0.83 29 708 716 8 578.14 90.44 30 708 726 18 251.05 70.78 31 708 819 111 107.55 0.01 32 708 824 116 107.52 0.00 33 709 821 112 107.53 0.01 34 709 823 114 107.52 0.01 35 709 824 115 107.50 0.00 36 709 872 163 70.23 1.83 37 709 933 224 71.42 1.14 38 709 937 228 68.78 1.15 39 710 868 158 69.97 1.71 40 710 871 161 70.11 1.77 41 710 872 162 70.23 1.83 42 710 937 227 69.39 1.09 43 711 824 113 107.75 0.10 44 712 726 14 255.90 69.06 45 712 727 15 251.64 71.09 46 712 728 16 251.25 71.12 47 712 730 18 250.88 70.94 48 712 824 112 244.56 68.21 49 713 726 13 257.19 69.68 50 713 727 14 251.64 71.10 51 713 728 15 251.64 71.10 52 714 725 11 262.31 68.29 53 714 726 12 262.18 68.34 54 715 718 3 929.62 1.22 55 715 723 8 263.10 67.98 56 715 724 9 262.76 68.11 57 715 725 10 262.31 68.28 58 716 724 8 262.93 68.05 59 716 726 10 262.39 68.25 60 716 816 100 263.07 67.96 61 716 817 101 262.90 68.02 62 716 818 102 262.76 68.06 63 716 819 103 262.30 68.29 64 717 723 6 390.16 4.50 65 717 724 7 263.10 67.96 66 717 814 97 447.05 8.60 67 717 816 99 263.07 67.96 68 718 722 4 768.30 0.14 69 718 723 5 390.16 4.50 70 718 744 26 582.10 0.17 71 719 722 3 843.99 2.56 72 719 744 25 582.21 0.15 73 719 814 95 514.98 2.11 74 720 744 24 582.21 0.14 75 721 743 22 582.21 0.15 76 721 744 23 582.21 0.14 77 721 745 24 582.20 0.14 78 721 749 28 581.91 0.07 79 721 805 84 576.08 0.76 80 721 807 86 576.23 0.78 81 721 812 91 575.63 0.77 82 722 741 19 582.69 0.04 83 722 742 20 582.51 0.02 84 722 744 22 582.50 0.01 85 722 749 27 582.19 0.14 86 722 752 30 581.61 0.20 87 722 803 81 573.13 0.93 88 722 805 83 576.48 0.67 89 722 814 92 572.07 0.68 90 723 740 17 583.17 0.01 91 723 741 18 583.10 0.04 92 723 742 19 582.69 0.04 93 723 744 21 582.69 0.04 94 724 739 15 583.22 0.01 95 724 740 16 583.17 0.01 96 724 741 17 583.12 0.03 97 724 801 77 575.17 0.53 98 724 803 79 576.51 0.68 99 724 814 90 576.16 0.80 100 724 816 92 574.30 0.45 101 725 739 14 583.24 0.01 102 725 740 15 583.19 0.01 103 726 737 11 583.38 0.01 104 726 738 12 583.30 0.00 105 726 739 13 583.25 0.00 106 726 818 92 575.47 0.68 107 726 819 93 569.44 2.76 108 727 737 10 583.39 0.00 109 727 738 11 583.37 0.01 110 727 740 13 583.36 0.01 111 728 736 8 583.50 0.01 112 728 737 9 583.40 0.00 113 729 735 6 583.58 0.01 114 729 736 7 583.52 0.01 115 730 734 4 757.19 2.06 116 730 735 5 583.58 0.02 117 730 789 59 583.65 0.05 118 730 824 94 548.78 0.27 119 730 825 95 548.38 0.34 120 731 734 3 834.02 1.68 121 732 825 93 548.79 0.27 122 732 894 162 301.83 0.36 123 733 790 57 669.13 0.38 124 733 791 58 668.86 0.34 125 733 825 92 548.84 0.29 126 733 894 161 302.10 0.49 127 733 895 162 302.75 0.77 128 733 896 163 306.00 1.03 129 734 789 55 669.75 0.32 130 734 790 56 669.49 0.20 131 734 825 91 548.79 0.27 132 735 788 53 669.77 0.32 133 735 789 54 669.75 0.32 134 736 786 50 670.65 0.46 135 736 787 51 670.27 0.56 136 736 788 52 669.78 0.33 137 736 789 53 670.01 0.44 138 737 786 49 670.67 0.47 139 737 787 50 670.38 0.53 140 737 789 52 670.35 0.51 141 737 818 81 576.03 0.89 142 737 819 82 575.88 0.84 143 737 820 83 571.12 0.20 144 738 784 46 671.25 0.58 145 738 785 47 671.00 0.61 146 738 786 48 670.93 0.59 147 739 756 17 670.40 0.33 148 739 784 45 671.29 0.59 149 739 785 46 671.25 0.58 150 739 787 48 671.00 0.62 151 739 801 62 669.39 0.49 152 739 818 79 575.49 0.67 153 740 782 42 671.95 0.73 154 740 783 43 671.81 0.69 155 740 784 44 671.46 0.67 156 741 752 11 671.64 0.60 157 741 754 13 670.42 0.15 158 741 782 41 671.96 0.73 159 741 783 42 671.82 0.69 160 741 785 44 671.81 0.69 161 741 801 60 670.16 0.45 162 741 803 62 669.85 0.39 163 742 781 39 672.15 0.67 164 742 782 40 671.97 0.73 165 743 747 4 880.95 1.57 166 743 748 5 803.06 12.23 167 743 749 6 757.14 3.71 168 743 777 34 672.64 0.79 169 743 778 35 680.62 4.42 170 743 779 36 678.99 3.62 171 743 780 37 672.76 0.81 172 743 781 38 672.70 0.83 173 745 749 4 894.22 4.76 174 745 779 34 745.97 0.13 175 745 780 35 736.96 4.63 176 745 807 62 669.01 0.45 177 746 779 33 745.98 0.13 178 747 779 32 751.73 2.35 179 747 807 60 668.92 0.50 180 748 776 28 752.49 2.58 181 748 777 29 752.07 2.42 182 748 778 30 752.00 2.44 183 748 779 31 751.93 2.39 184 749 752 3 892.39 0.21 185 749 775 26 752.60 2.54 186 749 776 27 752.58 2.56 187 749 777 28 752.21 2.46 188 749 805 56 669.83 0.49 189 749 806 57 669.33 0.70 190 749 807 58 669.00 0.58 191 750 775 25 752.60 2.54 192 750 776 26 752.60 2.55 193 750 805 55 669.84 0.50 194 750 806 56 669.30 0.71 195 751 775 24 752.62 2.55 196 751 804 53 670.14 0.51 197 751 805 54 669.83 0.49 198 751 806 55 669.34 0.73 199 752 773 21 752.62 2.56 200 752 775 23 752.78 2.48 201 752 777 25 752.98 2.50 202 752 783 31 752.93 2.48 203 752 803 51 670.19 0.52 204 752 804 52 670.01 0.57 205 752 805 53 669.32 0.73 206 753 803 50 670.19 0.52 207 753 804 51 670.15 0.52 208 754 773 19 752.99 2.48 209 754 785 31 752.94 2.47 210 754 801 47 670.63 0.68 211 754 802 48 670.56 0.69 212 754 803 49 670.16 0.54 213 755 800 45 670.91 0.74 214 755 801 46 670.60 0.70 215 755 802 47 670.57 0.70 216 756 768 12 753.12 2.94 217 756 770 14 753.01 2.51 218 756 787 31 753.01 2.50 219 756 800 44 670.88 0.76 220 756 801 45 670.72 0.76 221 756 818 62 582.11 0.01 222 757 799 42 670.93 0.75 223 757 800 43 670.85 0.78 224 757 802 45 670.83 0.77 225 758 762 4 829.25 6.99 226 758 763 5 821.09 2.92 227 758 768 10 753.65 2.79 228 758 799 41 670.90 0.74 229 759 762 3 848.34 0.82 230 759 763 4 847.34 1.21 231 760 763 3 856.08 3.06 232 760 764 4 856.08 3.06 233 761 764 3 858.21 2.00 234 761 765 4 849.70 0.03 235 762 765 3 855.96 3.12 236 762 766 4 849.66 0.03 237 762 795 33 715.76 0.84 238 762 797 35 672.74 0.14 239 762 798 36 671.21 0.61 240 762 799 37 671.13 0.63 241 763 766 3 863.48 0.77 242 764 792 28 752.91 2.47 243 765 791 26 752.97 2.48 244 765 792 27 752.87 2.46 245 765 793 28 752.79 2.43 246 765 794 29 752.28 2.42 247 765 795 30 748.88 2.39 248 766 789 23 753.28 2.56 249 766 790 24 753.07 2.48 250 766 791 25 752.95 2.50 251 766 795 29 745.75 3.27 252 766 799 33 670.50 0.60 253 766 820 54 576.29 0.79 254 766 825 59 562.15 0.56 255 767 789 22 753.32 2.54 256 767 790 23 753.10 2.48 257 767 792 25 752.95 2.46 258 768 787 19 753.54 2.52 259 768 788 20 753.43 2.52 260 768 789 21 753.32 2.54 261 768 799 31 670.34 0.52 262 768 820 52 575.98 0.89 263 769 787 18 753.54 2.52 264 769 788 19 753.49 2.50 265 769 790 21 753.43 2.52 266 770 785 15 753.68 2.49 267 770 786 16 753.63 2.50 268 770 787 17 753.56 2.51 269 773 783 10 753.86 2.48 270 773 784 11 753.86 2.48 271 773 785 12 753.79 2.46 272 777 781 4 808.76 2.50 273 777 783 6 754.26 2.52 274 778 781 3 856.32 3.25 275 789 820 31 575.87 0.85 276 789 825 36 548.84 0.29 277 790 896 106 306.01 1.03 278 791 795 4 870.52 0.96 279 791 825 34 551.22 0.91 280 791 895 104 305.42 0.80 281 791 896 105 306.06 1.01 282 792 895 103 306.00 1.03 283 792 896 104 306.07 1.01 284 792 897 105 306.08 1.01 285 793 895 102 306.07 1.01 286 793 897 104 306.12 1.01 287 793 923 130 199.96 0.35 288 794 822 28 576.25 0.80 289 794 895 101 306.17 1.02 290 794 923 129 193.36 0.01 291 794 925 131 177.60 3.00 292 794 926 132 129.18 6.20 293 795 820 25 582.10 0.23 294 795 821 26 580.27 0.35 295 795 822 27 576.25 0.80 296 795 825 30 575.97 0.91 297 795 929 134 71.72 1.21 298 796 929 133 71.59 1.22 299 796 932 136 71.61 1.23 300 798 817 19 582.84 0.03 301 798 818 20 582.79 0.03 302 799 817 18 582.86 0.03 303 799 818 19 582.75 0.06 304 799 820 21 581.02 0.21 305 800 815 15 583.17 0.05 306 800 816 16 583.07 0.03 307 800 817 17 582.84 0.02 308 801 815 14 583.11 0.01 309 801 816 15 582.95 0.03 310 801 818 17 581.77 0.26 311 802 813 11 583.56 0.04 312 802 814 12 583.41 0.01 313 802 815 13 583.11 0.01 314 803 813 10 583.53 0.03 315 803 814 11 583.43 0.01 316 803 816 13 583.29 0.07 317 804 812 8 583.76 0.01 318 804 813 9 583.54 0.03 319 805 811 6 583.97 0.04 320 805 812 7 583.79 0.02 321 806 809 3 858.86 1.00 322 806 810 4 634.62 25.33 323 806 811 5 584.00 0.03 324 807 810 3 847.75 2.79 325 807 812 5 584.03 0.04 326 819 824 5 854.82 2.30 327 820 825 5 854.82 2.31 328 821 824 3 870.19 2.85 329 821 825 4 871.52 2.18 330 821 929 108 71.71 1.19 331 821 933 112 71.61 1.23 332 822 825 3 879.80 1.96 333 822 893 71 305.45 0.80 334 822 926 104 107.81 16.86 335 822 929 107 71.73 1.19 336 822 930 108 71.70 1.19 337 822 933 111 71.71 1.21 338 823 826 3 860.52 0.55 339 823 872 49 257.69 4.54 340 823 893 70 306.16 0.94 341 823 930 107 71.70 1.18 342 823 933 110 71.73 1.20 343 825 893 68 307.41 1.01 344 825 894 69 307.42 1.01 345 826 872 46 305.69 1.24 346 826 873 47 305.65 1.23 347 826 874 48 305.83 1.16 348 826 891 65 307.24 1.09 349 826 892 66 307.42 1.01 350 826 893 67 307.44 1.01 351 826 902 76 307.44 1.01 352 826 930 104 180.50 4.38 353 827 891 64 307.45 1.02 354 827 892 65 307.48 1.02 355 827 894 67 307.81 0.97 356 828 873 45 307.22 0.96 357 828 874 46 307.22 0.96 358 828 875 47 307.18 0.95 359 828 890 62 307.52 1.01 360 828 891 63 307.55 1.02 361 829 875 46 307.74 1.00 362 829 877 48 307.79 1.01 363 829 890 61 307.73 1.00 364 829 891 62 307.74 1.00 365 829 892 63 307.83 0.97 366 830 875 45 307.76 0.99 367 830 877 47 307.79 1.01 368 831 835 4 841.52 0.66 369 831 836 5 825.51 3.26 370 831 839 8 818.21 1.65 371 831 877 46 307.85 0.98 372 831 890 59 307.85 0.98 373 832 835 3 853.29 2.94 374 832 836 4 845.12 1.14 375 833 836 3 845.34 1.03 376 833 837 4 841.47 2.69 377 833 879 46 307.89 1.01 378 833 880 47 307.83 1.00 379 834 837 3 870.95 3.15 380 834 838 4 841.47 2.69 381 835 838 3 862.05 7.59 382 835 839 4 845.83 0.52 383 836 839 3 852.58 2.86 384 836 840 4 844.93 0.95 385 836 877 41 308.18 0.93 386 836 880 44 308.17 0.92 387 836 888 52 308.14 0.93 388 836 889 53 308.15 0.93 389 836 903 67 308.20 0.89 390 837 840 3 843.17 0.07 391 837 841 4 834.44 4.12 392 837 880 43 308.18 0.92 393 838 841 3 850.62 3.79 394 838 842 4 842.85 0.09 395 839 842 3 844.63 2.00 396 839 903 64 308.24 0.89 397 840 843 3 879.75 4.42 398 840 903 63 308.25 0.89 399 840 905 65 308.25 0.89 400 840 918 78 216.76 2.10 401 840 920 80 205.50 0.70 402 843 848 5 555.71 61.88 403 843 849 6 425.60 7.42 404 843 920 77 216.47 2.16 405 845 848 3 859.64 3.16 406 846 849 3 863.22 2.44 407 847 924 77 211.25 0.04 408 848 920 72 247.21 0.90 409 848 921 73 243.28 1.02 410 848 922 74 243.27 1.02 411 848 923 75 216.45 2.19 412 848 924 76 203.62 1.88 413 848 927 79 202.34 1.24 414 849 918 69 278.45 1.22 415 849 919 70 271.25 2.31 416 849 920 71 248.34 1.47 417 850 918 68 278.45 1.22 418 850 919 69 278.36 1.25 419 850 927 77 200.50 0.33 420 850 928 78 200.46 0.33 421 850 931 81 199.33 0.36 422 851 916 65 302.50 1.59 423 851 917 66 301.01 0.87 424 851 918 67 278.67 1.32 425 852 916 64 302.50 1.59 426 852 917 65 302.42 1.55 427 852 931 79 200.22 0.36 428 852 935 83 196.91 0.77 429 853 914 61 303.30 1.72 430 853 915 62 303.06 1.77 431 853 916 63 302.50 1.59 432 854 914 60 303.32 1.71 433 854 915 61 303.24 1.72 434 854 917 63 303.09 1.79 435 854 935 81 198.60 0.60 436 854 938 84 192.81 0.67 437 855 913 58 303.37 1.72 438 855 914 59 303.28 1.75 439 856 860 4 822.61 3.18 440 856 913 57 303.37 1.74 441 856 914 58 303.28 1.76 442 856 915 59 303.16 1.82 443 856 938 82 192.75 0.70 444 857 935 78 198.38 0.83 445 857 936 79 197.79 1.06 446 857 938 81 192.77 0.69 447 858 868 10 612.03 84.53 448 858 937 79 192.91 0.64 449 858 938 80 192.66 0.66 450 860 908 48 309.35 0.95 451 860 909 49 309.35 0.95 452 860 910 50 309.10 0.96 453 860 913 53 302.83 2.15 454 860 914 54 302.83 2.15 455 860 915 55 302.77 2.15 456 861 864 3 857.33 2.54 457 861 865 4 826.26 3.77 458 862 865 3 857.33 2.54 459 862 866 4 830.32 1.75 460 863 866 3 851.75 5.32 461 863 867 4 837.29 0.80 462 864 867 3 838.35 1.32 463 864 868 4 832.14 1.79 464 865 868 3 848.26 5.90 465 865 869 4 837.27 0.78 466 865 887 22 310.51 1.17 467 865 906 41 310.48 1.17 468 865 938 73 182.90 0.34 469 866 869 3 854.63 2.73 470 866 870 4 837.27 0.78 471 866 876 10 360.42 2.41 472 866 878 12 349.79 4.23 473 866 887 21 310.49 1.16 474 867 870 3 840.37 1.89 475 867 871 4 823.50 2.53 476 868 871 3 837.72 0.56 477 868 872 4 815.65 1.37 478 868 937 69 183.49 0.04 479 868 938 70 182.88 0.34 480 869 872 3 828.91 0.23 481 869 873 4 819.98 1.01 482 869 876 7 389.69 12.29 483 869 887 18 311.06 0.97 484 869 906 37 313.87 0.48 485 869 938 69 182.88 0.34 486 870 873 3 845.32 3.79 487 870 876 6 392.98 13.93 488 872 930 58 196.74 0.49 489 872 933 61 192.41 0.87 490 872 934 62 192.22 0.82 491 874 889 15 337.48 2.56 492 874 890 16 337.44 2.58 493 874 891 17 337.34 2.63 494 874 904 30 337.44 2.58 495 875 889 14 337.48 2.56 496 875 890 15 337.49 2.56 497 876 887 11 337.48 2.56 498 876 888 12 337.50 2.55 499 877 880 3 883.91 3.42 500 877 888 11 337.72 2.64 501 877 889 12 338.81 2.98 502 877 890 13 340.33 3.50 503 880 888 8 341.31 3.15 504 881 886 5 388.12 13.22 505 881 888 7 345.99 0.83 506 881 905 24 341.34 3.13 507 883 886 3 862.62 6.45 508 885 908 23 342.62 2.93 509 886 905 19 343.33 2.75 510 886 906 20 343.07 2.78 511 886 907 21 342.66 2.91 512 886 908 22 342.67 2.91 513 887 905 18 343.33 2.75 514 887 906 19 343.22 2.71 515 887 908 21 343.17 2.73 516 888 904 16 343.37 2.75 517 888 905 17 343.32 2.73 518 889 903 14 343.49 2.80 519 889 904 15 343.44 2.78 520 890 902 12 344.49 2.54 521 890 903 13 343.58 2.83 522 891 901 10 353.07 3.82 523 891 902 11 352.20 4.17 524 892 899 7 380.40 6.98 525 892 900 8 364.04 1.18 526 892 901 9 353.14 3.85 527 893 899 6 381.92 7.69 528 893 900 7 380.36 6.96 529 893 902 9 363.63 1.37 530 893 926 33 198.97 0.07 531 893 930 37 190.47 0.61 532 894 899 5 398.25 1.91 533 895 898 3 827.50 9.26 534 895 899 4 628.24 89.02 535 895 900 5 628.24 89.02 536 900 921 21 200.45 0.37 537 900 922 22 200.28 0.35 538 900 923 23 200.27 0.35 539 900 926 26 200.21 0.34 540 901 921 20 200.44 0.37 541 901 922 21 200.34 0.38 542 902 920 18 200.71 0.33 543 902 921 19 200.44 0.37 544 902 926 24 200.24 0.35 545 902 930 28 197.91 0.27 546 903 919 16 200.86 0.33 547 903 920 17 200.71 0.33 548 904 917 13 210.09 1.50 549 904 918 14 209.19 1.18 550 904 919 15 200.86 0.33 551 904 934 30 192.73 0.67 552 905 917 12 211.56 0.19 553 905 918 13 209.19 1.18 554 906 915 9 355.38 4.37 555 906 916 10 288.94 36.93 556 906 917 11 212.86 0.83 557 906 938 32 182.86 0.34 558 907 915 8 355.39 4.37 559 907 916 9 292.25 35.79 560 908 914 6 376.60 6.92 561 908 915 7 357.92 3.12 562 909 912 3 847.12 5.26 563 909 913 4 782.31 6.96 564 909 914 5 400.62 5.06 565 910 913 3 827.62 1.38 566 915 938 23 192.59 0.68 567 917 931 14 199.30 0.38 568 917 934 17 195.71 0.14 569 917 935 18 192.78 0.65 570 919 927 8 358.63 8.84 571 919 930 11 199.34 0.35 572 919 931 12 198.02 0.90 573 921 926 5 802.28 6.87 574 921 927 6 409.82 3.54 575 921 930 9 414.62 4.39 576 923 926 3 839.36 2.29 577 923 927 4 833.76 0.49 578 924 927 3 841.25 2.88 579 924 928 4 840.88 3.06 580 925 928 3 840.89 3.06 581 925 929 4 839.05 3.98 582 926 929 3 852.48 0.61 583 926 930 4 832.93 0.51 584 927 930 3 855.43 1.02 585 927 931 4 826.57 3.69 586 928 931 3 848.36 4.45 587 928 932 4 833.94 0.01 588 929 932 3 836.49 7.99 589 929 933 4 831.46 5.48 590 930 933 3 845.32 3.58 591 930 934 4 835.10 1.59 592 931 934 3 848.95 1.76 593 931 935 4 843.12 2.42 594 932 935 3 858.88 3.29 595 932 936 4 851.13 2.86 596 933 936 3 856.67 4.44 597 933 937 4 851.47 3.03 598 934 937 3 856.10 4.71 599 934 938 4 852.84 6.34 600 935 938 3 867.36 0.91