BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 5 3 868.25 1.96 2 2 6 4 865.94 0.81 3 3 6 3 868.25 1.96 4 3 7 4 865.94 0.81 5 3 213 210 51.48 1.17 6 4 7 3 879.39 4.08 7 4 8 4 866.79 10.38 8 4 209 205 49.55 0.35 9 4 210 206 50.85 0.96 10 4 213 209 52.23 1.54 11 5 8 3 869.30 9.13 12 5 9 4 866.80 10.38 13 5 247 242 33.77 0.65 14 5 248 243 31.23 0.54 15 6 9 3 888.37 0.39 16 6 10 4 869.25 9.16 17 7 10 3 882.60 2.49 18 7 11 4 869.25 9.16 19 7 213 206 93.36 1.17 20 7 216 209 93.36 1.17 21 8 11 3 875.99 5.80 22 8 12 4 856.10 2.58 23 8 209 201 93.35 1.17 24 8 244 236 54.55 1.50 25 8 247 239 45.39 0.01 26 8 248 240 44.20 0.53 27 9 12 3 862.05 4.49 28 9 13 4 862.50 5.95 29 9 40 31 482.80 0.59 30 9 248 239 44.22 0.54 31 10 13 3 879.25 4.17 32 10 14 4 862.50 5.96 33 10 216 206 114.56 1.61 34 10 220 210 127.86 0.06 35 10 225 215 129.84 0.89 36 10 228 218 127.85 0.06 37 11 14 3 872.64 0.89 38 11 15 4 862.50 5.95 39 11 212 201 111.26 0.01 40 11 216 205 120.72 1.45 41 11 235 224 105.92 0.79 42 11 244 233 69.43 0.07 43 12 15 3 872.64 0.88 44 12 38 26 492.65 0.83 45 12 40 28 492.61 0.81 46 12 41 29 492.18 0.71 47 12 42 30 491.90 0.61 48 12 43 31 485.31 1.25 49 12 241 229 79.91 0.27 50 12 244 232 69.46 0.07 51 12 245 233 69.43 0.07 52 12 248 236 49.42 1.59 53 13 40 27 492.66 0.83 54 13 41 28 492.46 0.77 55 13 42 29 492.06 0.68 56 13 43 30 491.00 0.78 57 13 44 31 479.98 1.43 58 14 44 30 491.41 0.79 59 14 216 202 131.16 0.34 60 14 228 214 132.82 0.10 61 14 229 215 132.84 0.09 62 14 230 216 132.85 0.08 63 14 233 219 129.69 0.75 64 14 234 220 128.19 0.01 65 14 235 221 128.19 0.01 66 15 43 28 492.24 0.71 67 15 44 29 492.21 0.70 68 15 45 30 492.23 0.71 69 15 235 220 128.20 0.01 70 15 237 222 127.88 0.08 71 15 240 225 86.73 0.57 72 15 241 226 81.51 0.83 73 15 244 229 73.03 0.96 74 16 45 29 492.23 0.71 75 16 47 31 491.36 0.58 76 16 137 121 477.97 0.30 77 16 201 185 132.94 0.04 78 16 234 218 132.91 0.04 79 16 235 219 131.66 0.39 80 16 236 220 128.21 0.02 81 16 237 221 128.06 0.07 82 17 32 15 492.79 0.82 83 17 35 18 492.82 0.82 84 17 36 19 492.67 0.82 85 17 43 26 492.65 0.82 86 17 45 28 492.52 0.78 87 17 46 29 492.28 0.68 88 17 47 30 492.25 0.70 89 17 237 220 128.17 0.02 90 17 238 221 126.02 0.49 91 17 241 224 86.42 0.76 92 18 32 14 492.90 0.79 93 18 47 29 492.34 0.68 94 18 237 219 131.35 0.24 95 18 238 220 128.03 0.07 96 19 24 5 651.76 78.64 97 19 29 10 492.90 0.79 98 19 32 13 492.90 0.79 99 19 47 28 492.67 0.82 100 19 48 29 492.67 0.82 101 20 47 27 492.74 0.82 102 20 48 28 492.73 0.81 103 20 49 29 492.68 0.83 104 20 73 53 492.66 0.82 105 20 76 56 492.65 0.83 106 20 172 152 337.33 7.04 107 21 24 3 871.28 0.21 108 21 49 28 492.82 0.81 109 23 28 5 852.58 1.70 110 24 28 4 852.57 1.70 111 24 29 5 844.25 2.44 112 24 52 28 493.04 0.77 113 25 28 3 872.38 2.21 114 25 29 4 852.26 1.55 115 25 52 27 493.13 0.73 116 26 29 3 869.26 3.77 117 26 30 4 852.25 1.56 118 26 52 26 493.57 0.90 119 26 55 29 496.84 1.98 120 27 30 3 866.90 2.45 121 27 31 4 852.23 1.57 122 28 31 3 864.31 1.16 123 28 32 4 855.35 3.18 124 29 32 3 864.31 1.16 125 29 33 4 855.34 3.19 126 29 48 19 496.40 1.89 127 29 52 23 496.63 1.95 128 29 56 27 497.14 1.83 129 30 33 3 866.51 0.05 130 30 34 4 863.70 0.99 131 30 55 25 497.35 1.78 132 30 59 29 497.54 1.70 133 31 34 3 869.89 1.72 134 31 35 4 855.00 3.47 135 32 35 3 867.82 2.91 136 32 36 4 861.65 0.18 137 32 46 14 497.20 1.83 138 32 48 16 497.40 1.73 139 33 36 3 871.77 4.67 140 33 37 4 871.56 4.77 141 33 46 13 497.41 1.73 142 33 56 23 497.57 1.74 143 33 59 26 497.78 1.72 144 33 60 27 497.66 1.71 145 33 63 30 498.35 1.80 146 34 37 3 890.50 4.70 147 34 59 25 498.02 1.69 148 34 63 29 498.48 1.74 149 35 38 3 895.56 2.17 150 35 241 206 84.24 0.20 151 36 42 6 497.98 1.64 152 36 43 7 497.81 1.66 153 36 46 10 497.56 1.72 154 36 68 32 497.81 1.64 155 37 42 5 497.98 1.64 156 37 63 26 610.28 23.77 157 37 65 28 504.65 1.34 158 38 41 3 849.90 2.65 159 38 241 203 81.53 0.70 160 38 245 207 69.68 0.04 161 39 245 206 69.79 0.03 162 39 250 211 48.59 1.18 163 40 245 205 69.67 0.04 164 40 248 208 53.15 3.44 165 40 250 210 45.37 0.01 166 42 65 23 658.53 32.52 167 42 67 25 658.46 32.54 168 42 68 26 658.31 32.52 169 43 68 25 658.45 32.54 170 43 241 198 81.51 0.70 171 44 68 24 658.46 32.54 172 44 69 25 658.44 32.54 173 44 230 186 133.05 0.08 174 44 234 190 132.72 0.06 175 45 68 23 658.46 32.54 176 45 69 24 658.43 32.54 177 45 234 189 132.88 0.09 178 46 56 10 658.89 32.55 179 46 60 14 658.92 32.51 180 46 68 22 658.63 32.55 181 46 69 23 658.60 32.55 182 46 70 24 658.44 32.54 183 46 71 25 658.44 32.54 184 47 71 24 658.44 32.54 185 47 72 25 658.43 32.54 186 47 73 26 658.43 32.54 187 48 52 4 864.55 1.96 188 48 53 5 848.14 1.03 189 48 56 8 754.76 15.26 190 48 70 22 658.71 32.51 191 48 71 23 658.63 32.53 192 48 72 24 658.49 32.52 193 49 52 3 886.03 5.79 194 49 72 23 658.64 32.52 195 49 73 24 658.59 32.54 196 49 76 27 658.50 32.49 197 49 172 123 347.37 10.53 198 50 53 3 893.52 0.10 199 50 54 4 899.69 2.98 200 50 72 22 658.72 32.52 201 50 73 23 658.69 32.51 202 50 93 43 658.68 32.51 203 50 96 46 659.32 32.37 204 51 54 3 942.27 0.88 205 51 96 45 659.36 32.37 206 52 55 3 893.02 0.15 207 52 56 4 870.05 0.82 208 53 56 3 882.52 5.40 209 53 57 4 865.33 1.53 210 53 70 17 659.49 32.41 211 53 72 19 659.44 32.41 212 53 93 40 659.50 32.40 213 53 96 43 659.56 32.38 214 53 97 44 659.56 32.38 215 53 98 45 659.49 32.41 216 54 57 3 872.35 0.34 217 54 58 4 868.37 0.01 218 54 96 42 659.56 32.38 219 55 58 3 862.35 0.04 220 55 59 4 862.26 0.00 221 56 59 3 867.06 2.40 222 56 60 4 862.24 0.01 223 56 70 14 659.68 32.37 224 57 60 3 864.41 1.07 225 57 61 4 860.66 0.74 226 57 70 13 659.71 32.36 227 57 97 40 659.74 32.36 228 57 99 42 659.74 32.36 229 58 61 3 861.63 1.21 230 58 62 4 859.32 0.07 231 59 62 3 863.97 1.69 232 59 63 4 856.98 1.42 233 60 63 3 869.86 2.32 234 60 64 4 856.98 1.42 235 60 65 5 828.01 1.02 236 60 66 6 728.68 1.92 237 60 68 8 659.96 32.43 238 60 70 10 659.76 32.34 239 61 64 3 861.10 0.64 240 61 66 5 765.36 20.27 241 65 68 3 879.59 2.75 242 69 99 30 740.59 31.03 243 69 230 161 133.37 0.01 244 70 98 28 740.86 30.94 245 70 99 29 740.81 30.96 246 70 100 30 740.62 31.04 247 71 100 29 740.63 31.04 248 71 137 66 641.16 18.70 249 72 93 21 741.32 30.87 250 72 98 26 741.12 30.94 251 72 100 28 740.99 31.00 252 72 101 29 740.84 30.93 253 72 102 30 740.81 30.94 254 73 76 3 897.35 3.72 255 73 102 29 740.81 30.94 256 73 167 94 355.91 13.19 257 74 89 15 741.52 30.84 258 74 90 16 741.53 30.85 259 74 93 19 741.52 30.83 260 74 101 27 741.29 30.86 261 74 102 28 741.35 30.79 262 74 103 29 741.29 30.72 263 74 104 30 741.65 30.54 264 75 78 3 933.18 1.93 265 75 79 4 826.30 9.26 266 75 80 5 798.37 2.56 267 75 86 11 741.81 30.63 268 75 89 14 741.92 30.69 269 75 103 28 741.76 30.59 270 75 104 29 741.74 30.55 271 75 121 46 644.61 17.99 272 76 104 28 741.75 30.56 273 76 139 63 641.54 18.88 274 76 170 94 354.08 13.53 275 76 172 96 353.93 13.50 276 77 104 27 741.83 30.59 277 77 170 93 354.08 13.53 278 77 172 95 353.58 13.34 279 78 107 29 766.71 18.10 280 78 108 30 766.70 18.07 281 79 104 25 742.32 30.34 282 79 105 26 766.36 18.32 283 79 106 27 766.63 18.14 284 79 108 29 767.01 17.93 285 80 106 26 766.70 18.11 286 80 108 28 767.05 17.91 287 80 121 41 669.70 30.39 288 81 106 25 767.02 17.93 289 81 109 28 766.68 17.70 290 81 117 36 669.75 30.39 291 81 121 40 669.70 30.39 292 82 85 3 867.00 0.72 293 82 86 4 843.53 4.53 294 83 86 3 863.59 0.33 295 83 87 4 835.23 2.67 296 83 117 34 670.18 30.61 297 83 120 37 669.99 30.53 298 83 121 38 669.94 30.51 299 83 124 41 669.51 30.71 300 84 87 3 868.93 4.22 301 84 88 4 841.16 0.55 302 85 88 3 865.36 0.04 303 85 89 4 843.28 0.85 304 86 89 3 862.94 1.36 305 86 90 4 852.49 3.75 306 86 121 35 670.08 30.63 307 87 90 3 853.91 3.12 308 87 91 4 859.40 0.35 309 87 124 37 669.05 31.08 310 87 125 38 669.00 31.05 311 87 128 41 668.89 31.11 312 88 91 3 859.45 0.36 313 88 92 4 855.47 5.03 314 89 92 3 856.00 4.76 315 89 93 4 855.48 5.03 316 90 93 3 873.40 3.94 317 90 94 4 873.40 3.94 318 90 101 11 767.25 17.92 319 90 125 35 669.08 31.12 320 90 128 38 668.87 31.13 321 90 129 39 668.78 31.17 322 91 94 3 872.48 4.41 323 91 95 4 852.57 3.58 324 91 128 37 668.90 31.15 325 92 95 3 870.72 5.29 326 92 96 4 844.05 7.84 327 93 96 3 873.34 3.98 328 93 97 4 844.24 7.95 329 93 98 5 821.44 0.27 330 93 101 8 767.37 17.86 331 94 97 3 849.06 5.34 332 94 101 7 767.47 17.83 333 94 128 34 668.97 31.19 334 94 129 35 668.87 31.22 335 94 132 38 667.63 30.86 336 94 134 40 666.23 30.19 337 98 101 3 919.69 1.24 338 98 134 36 657.90 26.05 339 99 134 35 655.44 24.81 340 99 135 36 655.44 24.81 341 100 134 34 647.11 20.63 342 100 135 35 645.38 19.80 343 100 137 37 642.74 18.55 344 100 234 134 166.41 1.09 345 101 129 28 668.68 31.30 346 101 134 33 647.12 20.62 347 101 135 34 642.82 18.52 348 101 136 35 641.99 18.91 349 101 137 36 641.76 18.82 350 102 137 35 641.72 18.83 351 102 139 37 641.59 18.81 352 102 167 65 356.50 13.42 353 102 201 99 249.56 5.16 354 103 121 18 669.52 31.30 355 103 125 22 669.17 31.16 356 103 136 33 642.04 18.72 357 103 137 34 641.78 18.80 358 103 138 35 641.65 18.82 359 103 139 36 641.62 18.83 360 104 107 3 1011.58 6.12 361 104 139 35 641.47 18.84 362 104 170 66 354.28 13.51 363 105 110 5 869.83 2.54 364 105 118 13 670.39 30.89 365 105 122 17 669.74 31.19 366 105 138 33 641.73 18.80 367 105 139 34 641.69 18.82 368 105 140 35 641.44 18.82 369 105 154 49 362.85 11.62 370 105 157 52 362.81 11.61 371 105 158 53 362.75 11.58 372 106 109 3 874.80 3.03 373 106 110 4 872.80 4.03 374 106 117 11 670.57 30.82 375 106 118 12 670.50 30.84 376 106 121 15 670.55 30.82 377 107 110 3 869.85 2.53 378 107 111 4 869.78 2.52 379 107 140 33 641.63 18.79 380 107 141 34 641.58 18.77 381 107 144 37 633.93 19.32 382 107 145 38 610.81 20.86 383 108 111 3 869.83 2.54 384 108 112 4 869.76 2.54 385 109 112 3 869.78 2.52 386 109 113 4 869.76 2.54 387 109 114 5 787.51 9.58 388 109 117 8 670.64 30.83 389 110 113 3 863.59 5.62 390 110 114 4 798.62 2.84 391 110 115 5 673.30 32.02 392 110 145 35 640.98 18.74 393 110 150 40 386.50 0.43 394 110 153 43 375.17 5.86 395 110 154 44 364.14 11.34 396 110 157 47 364.04 11.39 397 111 144 33 641.43 18.77 398 111 145 34 639.12 19.64 399 111 150 39 386.51 0.42 400 114 117 3 902.56 6.54 401 115 118 3 877.28 2.06 402 115 119 4 853.60 1.91 403 115 153 38 375.44 6.00 404 115 157 42 364.03 11.40 405 116 119 3 863.39 1.42 406 116 120 4 858.33 3.27 407 117 120 3 860.74 4.48 408 117 121 4 856.82 0.48 409 118 121 3 869.74 0.21 410 118 122 4 860.38 2.25 411 118 157 39 362.87 11.62 412 119 122 3 864.74 4.42 413 119 123 4 856.82 0.47 414 119 157 38 362.87 11.62 415 119 160 41 362.81 11.60 416 119 161 42 362.31 11.68 417 120 123 3 861.84 1.30 418 120 124 4 855.94 0.03 419 121 124 3 865.82 3.29 420 121 125 4 851.18 4.14 421 122 125 3 861.28 1.02 422 122 126 4 862.03 3.75 423 122 136 14 642.86 18.48 424 122 138 16 642.07 18.77 425 122 158 36 362.79 11.60 426 122 161 39 361.47 11.33 427 122 163 41 359.63 12.19 428 123 126 3 868.90 0.34 429 123 127 4 862.05 3.75 430 123 161 38 361.31 11.40 431 124 127 3 863.75 2.91 432 124 128 4 864.38 2.61 433 125 128 3 877.15 0.48 434 125 129 4 856.06 6.78 435 125 136 11 642.85 18.48 436 126 129 3 873.86 2.12 437 126 130 4 856.03 6.78 438 126 136 10 642.86 18.48 439 126 162 36 359.45 12.21 440 126 164 38 359.42 12.20 441 127 130 3 869.49 0.64 442 127 131 4 864.38 2.60 443 128 131 3 874.85 3.31 444 128 132 4 856.53 5.83 445 129 132 3 856.52 5.84 446 129 133 4 847.20 6.17 447 129 134 5 645.93 20.00 448 129 136 7 642.87 18.50 449 130 133 3 845.40 7.07 450 135 164 29 359.07 12.33 451 135 165 30 359.07 12.33 452 135 231 96 167.12 1.07 453 136 163 27 359.34 12.21 454 136 164 28 359.08 12.33 455 136 165 29 359.07 12.33 456 137 165 28 356.95 13.38 457 137 201 64 249.83 5.19 458 137 234 97 166.09 1.05 459 138 158 20 362.47 11.56 460 138 163 25 359.33 12.22 461 138 165 27 357.45 13.14 462 138 166 28 356.74 13.29 463 138 167 29 356.74 13.29 464 139 167 28 356.43 13.37 465 139 168 29 356.22 13.26 466 139 169 30 355.76 13.09 467 139 170 31 355.41 12.93 468 140 145 5 828.33 0.99 469 140 151 11 363.44 11.37 470 140 154 14 362.84 11.62 471 140 155 15 362.83 11.62 472 140 158 18 362.79 11.60 473 140 167 27 356.66 13.33 474 140 185 45 326.80 0.42 475 141 144 3 867.31 0.49 476 141 145 4 848.02 8.85 477 141 168 27 356.47 13.40 478 141 169 28 356.07 13.23 479 141 170 29 355.95 13.18 480 141 173 32 353.60 13.18 481 142 145 3 861.33 7.29 482 142 146 4 821.97 2.18 483 142 151 9 365.72 10.44 484 142 168 26 356.41 13.39 485 142 169 27 356.41 13.39 486 142 181 39 327.45 0.46 487 142 185 43 326.95 0.36 488 143 175 32 327.87 0.42 489 144 173 29 354.33 13.62 490 145 150 5 562.25 88.42 491 145 151 6 386.13 0.37 492 145 154 9 364.06 11.40 493 147 150 3 860.36 1.08 494 147 151 4 843.53 6.56 495 148 151 3 855.78 1.44 496 148 152 4 843.55 6.57 497 148 184 36 327.53 0.47 498 149 152 3 846.06 6.03 499 149 153 4 843.56 6.57 500 150 153 3 845.35 5.67 501 150 154 4 843.65 6.62 502 151 154 3 862.29 2.10 503 151 155 4 843.60 6.66 504 151 181 30 327.50 0.47 505 151 184 33 327.45 0.45 506 151 185 34 327.14 0.38 507 152 155 3 858.25 0.09 508 152 156 4 843.61 6.67 509 152 184 32 327.42 0.45 510 152 187 35 326.85 0.41 511 152 188 36 326.13 0.58 512 153 156 3 848.51 4.78 513 153 157 4 843.60 6.67 514 154 157 3 862.68 1.89 515 154 158 4 843.38 6.78 516 155 158 3 863.85 1.11 517 155 159 4 845.72 7.95 518 155 166 11 357.00 13.40 519 155 185 30 326.82 0.41 520 155 188 33 324.36 0.23 521 156 159 3 864.03 1.21 522 156 160 4 841.52 10.04 523 156 188 32 324.37 0.23 524 156 192 36 311.44 0.93 525 157 160 3 852.11 4.75 526 157 161 4 844.31 0.85 527 158 161 3 860.74 9.06 528 158 162 4 833.57 6.23 529 158 163 5 686.93 32.10 530 158 166 8 359.36 12.22 531 159 162 3 866.11 6.38 532 159 163 4 783.82 20.57 533 159 166 7 359.42 12.26 534 159 188 29 324.36 0.23 535 159 189 30 324.25 0.22 536 159 192 33 306.43 1.54 537 159 194 35 260.82 5.19 538 159 198 39 251.24 5.08 539 160 192 32 308.99 0.28 540 163 166 3 902.87 2.66 541 163 198 35 251.17 5.11 542 164 197 33 251.19 5.09 543 164 198 34 251.14 5.10 544 164 199 35 251.12 5.09 545 164 231 67 167.13 1.07 546 165 198 33 251.14 5.10 547 165 199 34 250.44 5.34 548 165 200 35 250.41 5.34 549 165 201 36 250.19 5.26 550 165 230 65 167.40 0.97 551 165 231 66 167.20 1.04 552 165 232 67 167.04 1.08 553 165 233 68 166.99 1.07 554 165 234 69 166.81 1.02 555 166 185 19 326.72 0.40 556 166 189 23 324.25 0.23 557 166 198 32 251.15 5.10 558 166 199 33 250.53 5.38 559 166 200 34 250.12 5.29 560 166 201 35 249.86 5.17 561 167 185 18 326.70 0.41 562 167 201 34 249.11 5.01 563 167 203 36 245.85 5.25 564 168 177 9 327.67 0.39 565 168 185 17 326.68 0.42 566 168 200 32 250.19 5.30 567 168 201 33 249.14 5.03 568 168 202 34 246.74 4.80 569 168 203 35 244.33 5.73 570 169 176 7 327.67 0.39 571 169 177 8 327.67 0.39 572 169 203 34 241.06 5.58 573 171 176 5 327.60 0.42 574 171 204 33 213.16 2.46 575 173 176 3 916.45 0.19 576 175 178 3 1037.21 7.07 577 176 203 27 244.59 5.86 578 176 204 28 240.93 5.62 579 177 202 25 246.75 4.80 580 177 203 26 244.61 5.87 581 177 204 27 244.25 5.70 582 177 206 29 239.25 6.48 583 177 215 38 204.94 1.64 584 178 181 3 853.05 0.65 585 178 182 4 833.12 8.10 586 178 215 37 204.94 1.64 587 179 182 3 860.70 2.48 588 179 183 4 839.37 4.97 589 179 215 36 204.94 1.64 590 179 218 39 204.87 1.68 591 180 183 3 874.51 4.37 592 180 184 4 839.37 4.96 593 181 184 3 872.07 2.75 594 181 185 4 839.36 4.96 595 182 185 3 854.38 2.16 596 182 186 4 853.96 2.37 597 182 200 18 250.40 5.34 598 182 215 33 204.94 1.65 599 182 218 36 204.85 1.67 600 183 186 3 858.05 0.32 601 183 187 4 836.89 2.18 602 183 218 35 204.86 1.68 603 183 221 38 204.44 1.50 604 184 187 3 858.06 0.33 605 184 188 4 858.04 0.34 606 185 188 3 860.49 0.89 607 185 189 4 844.96 6.21 608 185 200 15 250.41 5.34 609 186 189 3 855.79 0.80 610 186 190 4 844.93 6.21 611 186 200 14 250.56 5.40 612 186 218 32 204.82 1.66 613 186 219 33 204.41 1.49 614 186 222 36 201.02 1.43 615 186 223 37 200.13 1.86 616 186 227 41 169.17 0.66 617 186 233 47 167.13 1.07 618 187 190 3 853.74 1.21 619 187 191 4 837.57 2.54 620 187 222 35 201.03 1.43 621 188 191 3 841.93 4.70 622 188 192 4 841.90 4.71 623 189 192 3 866.07 5.95 624 189 193 4 837.89 6.70 625 189 194 5 334.16 36.23 626 189 195 6 251.44 5.12 627 189 198 9 251.27 5.11 628 189 199 10 251.25 5.11 629 189 200 11 251.21 5.12 630 189 223 34 200.13 1.86 631 190 193 3 837.90 6.70 632 190 222 32 200.19 1.85 633 190 223 33 200.18 1.85 634 194 197 3 854.85 0.94 635 194 198 4 820.14 5.14 636 195 223 28 200.01 1.81 637 195 227 32 169.17 0.66 638 195 232 37 167.12 1.06 639 196 232 36 167.86 0.72 640 197 231 34 167.22 1.04 641 198 227 29 168.72 0.86 642 199 227 28 168.71 0.86 643 199 232 33 167.02 1.08 644 200 227 27 168.69 0.85 645 200 232 32 167.12 1.06 646 200 233 33 166.96 1.08 647 200 234 34 166.95 1.08 648 201 234 33 163.43 1.45 649 201 236 35 156.66 1.53 650 202 215 13 204.80 1.65 651 202 233 31 167.02 1.09 652 202 234 32 166.69 1.07 653 202 235 33 159.83 0.19 654 202 236 34 156.59 1.57 655 203 236 33 153.21 0.07 656 204 236 32 156.62 1.61 657 206 211 5 817.08 6.09 658 206 215 9 284.14 41.11 659 206 235 29 164.65 2.10 660 206 237 31 153.32 0.03 661 206 240 34 142.82 1.80 662 207 211 4 847.26 2.43 663 207 239 32 153.28 0.01 664 207 240 33 149.67 1.62 665 207 243 36 110.59 12.98 666 208 211 3 862.85 2.93 667 208 212 4 847.25 2.43 668 208 240 32 153.02 0.06 669 208 243 35 108.58 13.99 670 209 212 3 863.12 2.79 671 209 213 4 849.34 3.48 672 209 240 31 153.46 0.04 673 209 243 34 136.34 0.10 674 209 244 35 79.23 0.61 675 209 247 38 67.34 1.04 676 210 213 3 852.88 2.33 677 210 214 4 849.33 3.48 678 211 214 3 853.63 1.96 679 211 215 4 853.15 1.72 680 212 215 3 853.35 1.81 681 212 216 4 853.17 1.73 682 212 235 23 166.91 0.99 683 212 240 28 156.62 1.45 684 212 244 32 79.23 0.61 685 213 216 3 869.20 0.65 686 213 217 4 840.71 0.05 687 214 217 3 872.39 1.26 688 214 218 4 851.15 1.72 689 215 218 3 878.21 5.97 690 215 219 4 862.50 3.96 691 215 233 18 167.97 0.74 692 215 235 20 167.08 1.05 693 216 219 3 875.15 2.38 694 216 220 4 862.49 3.96 695 216 228 12 168.44 0.84 696 216 233 17 167.99 0.74 697 216 235 19 167.73 0.77 698 217 220 3 870.64 2.19 699 217 221 4 842.95 5.82 700 218 221 3 842.91 5.79 701 218 222 4 842.90 5.79 702 219 222 3 872.90 5.59 703 219 223 4 817.52 3.06 704 219 224 5 817.51 3.06 705 219 227 8 169.06 0.70 706 219 228 9 168.68 0.86 707 219 233 14 168.00 0.74 708 220 224 4 568.01 87.90 709 220 225 5 272.13 35.19 710 223 227 4 585.96 39.11 711 224 227 3 862.92 5.80 712 227 232 5 542.38 98.03 713 227 233 6 188.33 10.61 714 228 233 5 188.06 10.74 715 229 232 3 860.34 1.75 716 229 233 4 688.31 27.71 717 230 233 3 867.46 1.81 718 230 234 4 841.29 11.27 719 235 240 5 821.32 0.10 720 237 240 3 897.28 0.96 721 238 241 3 897.29 0.96 722 239 242 3 897.30 0.97 723 239 243 4 852.35 0.62 724 240 243 3 874.03 5.24 725 240 244 4 851.89 0.40 726 241 244 3 855.84 3.86 727 241 245 4 841.37 5.67 728 242 245 3 856.17 4.73 729 242 246 4 825.77 2.12 730 243 246 3 851.49 1.62 731 243 247 4 842.31 6.20 732 244 247 3 851.38 1.67 733 244 248 4 842.31 6.20 734 245 248 3 851.38 1.67 735 245 249 4 851.38 1.67 736 245 250 5 443.50 33.79 737 246 249 3 848.53 0.24 738 246 250 4 651.70 67.21