BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 19 48 29 21.84 0.09 2 19 49 30 25.25 0.74 3 19 51 32 25.31 0.71 4 19 164 145 23.09 0.40 5 19 167 148 22.98 0.34 6 19 168 149 22.52 0.11 7 21 49 28 30.67 0.14 8 21 51 30 30.27 0.03 9 22 51 29 30.68 0.15 10 22 53 31 32.76 0.10 11 22 56 34 40.94 0.61 12 22 59 37 39.33 0.88 13 22 164 142 32.24 0.04 14 23 51 28 32.62 0.16 15 23 52 29 32.62 0.16 16 23 53 30 38.74 1.06 17 23 56 33 41.38 0.61 18 24 53 29 39.70 0.71 19 24 54 30 41.15 0.72 20 24 56 32 41.54 0.65 21 25 28 3 856.79 1.63 22 25 52 27 40.38 0.80 23 25 53 28 41.36 0.61 24 25 54 29 41.38 0.61 25 26 29 3 856.79 1.63 26 27 30 3 856.93 1.56 27 28 31 3 843.76 7.88 28 28 32 4 821.16 0.03 29 28 41 13 50.80 0.34 30 28 43 15 50.76 0.35 31 28 52 24 50.81 0.34 32 28 53 25 50.81 0.34 33 28 54 26 50.81 0.34 34 28 257 229 44.18 0.06 35 28 261 233 42.24 1.03 36 29 32 3 825.78 0.75 37 29 33 4 816.31 0.02 38 29 54 25 50.84 0.34 39 30 33 3 814.64 0.85 40 30 34 4 813.52 0.33 41 31 34 3 824.15 1.47 42 31 35 4 813.68 0.38 43 31 253 222 51.23 0.20 44 31 256 225 51.23 0.20 45 31 257 226 51.74 0.06 46 32 35 3 831.84 8.70 47 32 36 4 746.78 22.25 48 32 37 5 689.73 32.33 49 32 39 7 585.37 9.29 50 32 40 8 581.16 7.19 51 32 41 9 560.79 17.37 52 32 54 22 93.77 17.07 53 32 237 205 59.97 0.17 54 32 253 221 57.02 1.33 55 32 257 225 56.99 1.31 56 33 36 3 801.14 6.53 57 35 249 214 60.55 0.13 58 35 253 218 59.04 0.63 59 37 239 202 119.62 2.45 60 37 249 212 103.62 0.95 61 37 253 216 97.81 3.85 62 38 58 20 149.47 0.35 63 38 239 201 119.62 2.46 64 38 240 202 119.61 2.44 65 39 54 15 181.94 0.23 66 39 58 19 160.07 4.95 67 39 238 199 126.49 0.88 68 39 239 200 119.62 2.46 69 40 53 13 387.23 102.86 70 40 54 14 181.94 0.23 71 40 55 15 181.47 0.23 72 40 58 18 167.06 1.46 73 40 59 19 162.84 1.71 74 40 163 123 147.84 6.08 75 40 237 197 134.37 2.27 76 40 238 198 134.23 2.27 77 41 53 12 181.99 0.23 78 41 54 13 181.97 0.23 79 41 235 194 181.54 0.42 80 41 236 195 179.59 0.07 81 41 237 196 149.02 5.04 82 41 254 213 110.74 1.39 83 41 257 216 103.65 0.81 84 41 258 217 103.62 0.82 85 42 51 9 864.60 0.75 86 42 52 10 735.95 21.64 87 42 53 11 559.84 20.09 88 42 59 17 181.72 0.34 89 42 163 121 181.50 0.43 90 42 164 122 181.77 0.33 91 42 167 125 181.78 0.32 92 42 235 193 181.57 0.44 93 42 236 194 181.46 0.46 94 43 51 8 954.85 7.15 95 43 52 9 826.22 0.08 96 43 233 190 190.12 0.10 97 43 234 191 184.32 1.55 98 43 235 192 181.58 0.45 99 43 261 218 100.16 0.47 100 43 263 220 91.80 0.05 101 44 167 123 195.04 2.37 102 44 233 189 194.88 1.31 103 44 234 190 188.69 0.62 104 45 231 186 209.66 0.07 105 45 232 187 209.65 0.07 106 45 233 188 207.94 0.01 107 46 231 185 209.66 0.07 108 46 232 186 209.65 0.07 109 46 234 188 209.61 0.04 110 47 171 124 209.66 0.07 111 47 234 187 209.66 0.07 112 48 167 119 209.65 0.07 113 48 168 120 209.66 0.07 114 48 171 123 209.69 0.07 115 51 167 116 208.49 0.25 116 53 56 3 855.41 5.25 117 53 59 6 671.62 42.16 118 55 58 3 825.73 1.75 119 55 59 4 815.95 0.43 120 56 59 3 836.95 7.35 121 57 60 3 847.81 1.92 122 57 61 4 817.81 1.34 123 58 61 3 817.81 1.34 124 58 63 5 817.86 1.32 125 58 238 180 209.86 0.01 126 58 239 181 209.75 0.04 127 58 240 182 209.75 0.04 128 59 162 103 353.30 32.35 129 59 163 104 324.49 28.03 130 59 164 105 324.49 28.02 131 60 162 102 357.49 33.34 132 60 164 104 351.89 32.18 133 61 162 101 389.75 23.89 134 62 143 81 417.51 28.44 135 62 160 98 418.01 28.68 136 62 161 99 400.13 19.89 137 62 162 100 389.78 23.91 138 63 160 97 420.98 28.43 139 63 161 98 417.57 28.44 140 63 219 156 267.33 26.21 141 63 221 158 280.00 19.82 142 63 238 175 231.19 1.22 143 64 153 89 439.00 24.41 144 64 154 90 438.67 24.26 145 64 155 91 439.17 24.48 146 64 156 92 439.07 24.44 147 64 157 93 438.50 24.62 148 64 159 95 422.67 27.75 149 64 160 96 422.59 27.72 150 64 209 145 272.21 23.84 151 64 219 155 270.50 24.64 152 64 221 157 280.18 19.78 153 65 68 3 826.04 2.69 154 65 69 4 742.16 26.98 155 65 141 76 433.36 22.41 156 65 154 89 439.15 24.48 157 65 159 94 432.27 26.05 158 65 160 95 423.87 28.28 159 65 161 96 422.73 27.73 160 65 221 156 284.75 17.50 161 66 69 3 847.70 4.12 162 66 154 88 439.54 24.29 163 66 207 141 280.29 19.83 164 66 208 142 280.30 19.84 165 66 209 143 279.16 20.40 166 67 70 3 844.88 1.43 167 67 115 48 547.82 18.72 168 67 205 138 285.95 17.00 169 67 206 139 285.79 17.09 170 67 207 140 280.30 19.84 171 68 71 3 851.52 1.90 172 68 72 4 844.14 1.05 173 68 166 98 427.99 29.60 174 68 205 137 285.95 17.00 175 68 206 138 286.45 17.34 176 68 207 139 280.88 20.13 177 68 221 153 287.38 18.16 178 68 223 155 285.95 17.00 179 69 72 3 844.14 1.05 180 69 73 4 819.71 2.39 181 69 132 63 476.69 27.00 182 69 136 67 476.41 27.05 183 69 141 72 476.01 27.16 184 69 153 84 471.30 27.00 185 69 154 85 471.18 26.94 186 69 159 90 466.02 28.46 187 70 73 3 826.53 1.02 188 70 74 4 816.51 0.85 189 70 115 45 548.57 18.83 190 70 117 47 548.49 18.84 191 70 120 50 548.38 18.82 192 70 154 84 471.71 26.78 193 70 155 85 471.70 26.77 194 70 205 135 289.66 18.82 195 71 74 3 826.48 0.99 196 71 75 4 813.97 0.58 197 71 169 98 428.06 29.59 198 71 170 99 428.00 29.59 199 71 173 102 417.85 26.12 200 71 188 117 359.42 27.15 201 71 192 121 357.05 27.23 202 71 205 134 289.68 18.82 203 71 223 152 289.68 18.81 204 72 75 3 815.49 1.34 205 72 76 4 812.23 1.15 206 72 136 64 476.44 27.06 207 72 139 67 476.43 27.07 208 72 141 69 476.32 27.11 209 72 169 97 428.06 29.59 210 73 76 3 819.03 0.42 211 73 77 4 819.02 0.41 212 73 120 47 548.54 18.85 213 73 123 50 548.08 18.89 214 73 124 51 548.04 18.89 215 73 154 81 471.74 26.77 216 74 77 3 819.02 0.41 217 74 78 4 819.02 0.42 218 74 83 9 632.34 57.32 219 74 108 34 548.85 18.85 220 74 112 38 548.76 18.87 221 74 120 46 548.54 18.85 222 74 188 114 359.46 27.13 223 75 78 3 819.07 0.42 224 75 79 4 817.59 1.16 225 75 139 64 476.73 27.09 226 75 169 94 429.03 29.12 227 75 173 98 419.34 26.38 228 75 178 103 368.03 29.55 229 75 180 105 367.68 29.46 230 75 188 113 359.50 27.12 231 76 79 3 822.40 1.25 232 76 80 4 808.86 3.21 233 76 127 51 548.06 18.89 234 76 132 56 542.64 18.16 235 76 135 59 477.92 27.66 236 76 136 60 500.55 26.00 237 76 139 63 476.75 27.07 238 77 80 3 822.40 1.25 239 77 81 4 813.67 5.61 240 77 82 5 720.88 31.29 241 77 83 6 632.33 57.32 242 77 123 46 548.35 18.76 243 77 127 50 548.06 18.88 244 78 81 3 827.75 1.43 245 78 83 5 682.16 32.41 246 78 85 7 552.13 19.94 247 78 86 8 552.12 19.93 248 78 108 30 549.23 18.90 249 78 184 106 362.28 26.82 250 78 187 109 362.27 26.82 251 78 188 110 361.48 26.57 252 79 135 56 538.29 20.36 253 79 180 101 367.68 29.46 254 79 181 102 367.68 29.46 255 79 184 105 363.51 27.44 256 80 127 47 548.12 18.87 257 80 131 51 547.38 18.66 258 80 135 55 542.72 18.21 259 82 123 41 548.69 18.85 260 82 126 44 548.40 18.80 261 82 127 45 548.37 18.79 262 82 131 49 548.03 18.81 263 83 88 5 586.98 29.86 264 83 90 7 563.63 23.91 265 83 108 25 549.27 18.89 266 83 123 40 548.69 18.85 267 84 88 4 717.31 34.26 268 85 88 3 815.77 0.47 269 86 187 101 361.58 26.50 270 88 107 19 552.83 19.11 271 89 105 16 552.94 19.10 272 89 106 17 552.86 19.11 273 90 105 15 552.97 19.09 274 90 106 16 552.90 19.11 275 90 107 17 552.93 19.11 276 90 108 18 552.90 19.12 277 90 119 29 549.12 18.81 278 90 123 33 549.12 18.80 279 91 105 14 553.00 19.09 280 92 95 3 912.43 11.00 281 92 97 5 800.98 0.81 282 92 103 11 553.06 19.07 283 92 104 12 553.09 19.09 284 92 105 13 553.01 19.09 285 96 103 7 556.88 17.84 286 97 102 5 709.07 21.02 287 97 103 6 567.10 22.14 288 97 118 21 553.17 19.11 289 97 119 22 553.52 19.07 290 98 102 4 831.66 2.64 291 99 102 3 834.82 1.06 292 99 103 4 831.66 2.64 293 100 103 3 832.95 2.00 294 101 104 3 896.82 3.20 295 101 114 13 557.56 20.45 296 102 106 4 845.31 0.70 297 102 114 12 557.58 20.46 298 102 115 13 557.57 20.45 299 102 116 14 555.32 19.43 300 102 117 15 555.42 19.39 301 102 118 16 555.31 19.43 302 104 114 10 600.27 32.67 303 106 110 4 737.03 31.25 304 106 111 5 723.08 24.28 305 106 114 8 693.75 9.71 306 106 116 10 564.07 21.38 307 106 119 13 560.21 19.89 308 107 110 3 801.56 5.87 309 107 111 4 807.36 4.09 310 108 111 3 819.82 0.17 311 108 112 4 809.67 5.24 312 108 120 12 572.84 26.14 313 108 187 79 361.36 26.53 314 108 191 83 358.92 27.26 315 109 112 3 820.13 0.01 316 109 113 4 809.68 5.24 317 109 191 82 358.89 27.25 318 109 193 84 358.89 27.25 319 110 113 3 819.12 0.45 320 110 114 4 807.38 4.08 321 111 114 3 843.06 1.30 322 111 115 4 733.06 21.97 323 111 116 5 662.60 5.83 324 111 119 8 600.59 21.88 325 111 120 9 605.74 24.45 326 112 115 3 810.41 2.52 327 112 120 8 621.32 32.24 328 112 191 79 358.89 27.25 329 112 192 80 358.85 27.25 330 112 205 93 289.75 18.78 331 113 191 78 358.89 27.25 332 113 192 79 358.89 27.25 333 113 194 81 357.29 27.49 334 115 205 90 289.66 18.79 335 116 119 3 837.56 6.76 336 116 120 4 815.42 0.68 337 117 120 3 815.42 0.68 338 117 121 4 809.71 2.17 339 117 155 38 471.77 26.75 340 118 121 3 815.01 0.48 341 118 122 4 814.11 0.03 342 119 122 3 826.88 3.32 343 119 123 4 814.09 0.04 344 120 123 3 818.71 2.35 345 120 124 4 814.09 0.04 346 120 154 34 472.16 26.66 347 121 124 3 821.66 3.83 348 121 125 4 821.16 3.58 349 121 151 30 472.14 26.62 350 121 154 33 472.20 26.65 351 121 155 34 472.15 26.66 352 122 125 3 824.44 2.44 353 122 126 4 813.86 0.07 354 123 126 3 821.50 3.91 355 123 127 4 814.68 0.49 356 124 127 3 814.69 0.49 357 124 128 4 814.68 0.49 358 124 132 8 707.28 30.21 359 124 150 26 472.55 26.62 360 124 151 27 472.54 26.62 361 124 154 30 472.44 26.62 362 125 128 3 814.68 0.49 363 125 150 25 472.67 26.57 364 125 151 26 472.56 26.62 365 126 131 5 825.64 3.70 366 127 131 4 806.54 3.36 367 127 132 5 806.52 3.37 368 128 131 3 838.89 2.04 369 128 132 4 821.38 4.03 370 128 150 22 472.95 26.66 371 129 132 3 825.64 3.69 372 129 133 4 823.18 4.92 373 129 146 17 475.78 27.25 374 129 150 21 472.97 26.67 375 130 133 3 830.55 1.25 376 130 134 4 821.85 3.79 377 131 134 3 817.93 1.11 378 131 135 4 813.79 0.23 379 132 135 3 827.61 0.83 380 132 136 4 811.66 0.83 381 132 146 14 476.10 27.10 382 132 150 18 472.96 26.66 383 132 153 21 472.70 26.71 384 133 136 3 826.30 3.11 385 133 137 4 820.73 2.51 386 133 144 11 476.37 27.20 387 133 146 13 476.12 27.11 388 134 137 3 825.50 3.70 389 134 138 4 808.80 2.10 390 135 138 3 824.55 4.17 391 135 139 4 798.63 2.90 392 135 179 44 367.91 29.59 393 136 139 3 807.07 9.34 394 136 140 4 781.93 5.45 395 136 141 5 673.88 15.77 396 136 144 8 476.39 27.22 397 136 146 10 476.36 27.23 398 136 153 17 472.53 26.64 399 136 159 23 471.32 26.61 400 137 140 3 771.53 28.56 401 137 144 7 476.60 27.23 402 138 179 41 367.89 29.61 403 139 169 30 429.07 29.14 404 139 178 39 367.93 29.60 405 139 179 40 367.92 29.59 406 140 144 4 820.42 0.15 407 140 165 25 437.10 25.79 408 141 144 3 825.26 2.27 409 141 159 18 471.32 26.61 410 141 161 20 454.92 25.18 411 141 169 28 429.07 29.14 412 142 160 18 470.94 26.46 413 142 161 19 448.20 22.81 414 142 165 23 437.16 25.82 415 143 158 15 471.59 26.71 416 143 159 16 471.33 26.62 417 143 160 17 471.13 26.54 418 144 159 15 471.33 26.61 419 145 158 13 471.59 26.71 420 145 159 14 471.33 26.61 421 146 153 7 472.38 26.68 422 147 153 6 647.18 42.17 423 147 158 11 471.75 26.76 424 148 152 4 833.83 0.70 425 149 152 3 849.00 3.04 426 149 153 4 813.41 4.91 427 150 153 3 876.11 4.14 428 150 154 4 865.84 0.99 429 151 154 3 952.38 3.64 430 151 155 4 853.60 0.73 431 152 155 3 832.57 2.18 432 152 156 4 820.69 0.35 433 152 157 5 728.01 35.30 434 153 157 4 631.72 41.39 435 153 159 6 563.27 22.34 436 154 159 5 632.62 34.88 437 161 165 4 797.18 0.84 438 161 166 5 725.03 22.48 439 161 221 60 289.13 19.02 440 162 165 3 798.69 0.09 441 162 166 4 744.53 25.48 442 162 221 59 289.21 19.04 443 163 166 3 810.21 4.21 444 163 167 4 799.83 0.97 445 163 221 58 289.39 18.95 446 163 236 73 246.28 2.51 447 163 238 75 245.21 1.99 448 164 167 3 813.74 3.48 449 164 168 4 795.62 3.00 450 165 168 3 813.74 3.48 451 165 169 4 798.33 1.66 452 166 169 3 823.43 5.88 453 166 170 4 800.75 0.85 454 166 221 55 289.66 18.84 455 166 223 57 289.45 18.72 456 166 236 70 246.64 2.66 457 167 170 3 825.51 4.83 458 167 171 4 806.12 1.82 459 167 234 67 247.22 2.50 460 167 235 68 247.15 2.48 461 167 236 69 246.69 2.65 462 168 171 3 823.28 3.46 463 168 172 4 812.25 1.24 464 169 172 3 827.58 1.43 465 169 173 4 806.31 1.73 466 169 178 9 474.19 82.71 467 170 173 3 814.70 2.46 468 170 174 4 806.31 1.73 469 170 223 53 289.78 18.79 470 170 224 54 289.71 18.75 471 170 225 55 289.43 18.57 472 171 174 3 830.98 5.67 473 171 175 4 806.30 1.72 474 171 234 63 247.31 2.48 475 172 175 3 815.00 7.92 476 172 176 4 781.39 1.91 477 172 177 5 678.58 41.70 478 172 178 6 617.38 11.11 479 173 176 3 800.72 1.08 480 173 178 5 624.65 14.73 481 173 185 12 361.06 26.65 482 173 189 16 359.42 27.16 483 173 192 19 359.07 27.27 484 173 225 52 291.94 17.70 485 174 189 15 359.50 27.19 486 174 225 51 291.94 17.71 487 174 227 53 291.94 17.59 488 174 232 58 249.69 3.52 489 174 234 60 249.53 3.52 490 176 182 6 367.46 29.41 491 176 185 9 361.50 26.44 492 176 189 13 361.33 26.52 493 177 185 8 578.64 13.54 494 178 185 7 630.07 39.26 495 180 185 5 798.46 0.18 496 181 184 3 826.23 9.15 497 181 185 4 800.40 0.69 498 182 185 3 807.20 0.23 499 182 186 4 802.88 1.93 500 183 186 3 819.88 0.30 501 183 187 4 809.75 5.37 502 184 187 3 825.95 2.73 503 184 188 4 809.98 5.25 504 185 188 3 820.18 5.38 505 185 189 4 804.45 2.48 506 186 189 3 815.44 3.01 507 186 190 4 809.98 5.25 508 187 190 3 836.38 0.97 509 187 191 4 827.40 3.46 510 188 191 3 827.51 3.41 511 188 192 4 827.40 3.46 512 189 192 3 827.51 3.41 513 189 193 4 827.51 3.41 514 189 203 14 291.88 17.77 515 189 225 36 291.98 17.71 516 190 193 3 832.96 0.69 517 192 203 11 290.79 18.32 518 192 204 12 290.07 18.70 519 192 205 13 289.99 18.79 520 192 225 33 291.95 17.72 521 193 201 8 292.25 17.75 522 193 202 9 292.13 17.79 523 193 203 10 291.17 18.17 524 194 202 8 292.10 17.75 525 194 204 10 291.95 17.80 526 195 200 5 664.72 51.09 527 195 201 6 292.93 18.08 528 195 202 7 292.21 17.77 529 196 200 4 690.16 38.38 530 196 201 5 294.07 18.65 531 197 200 3 810.61 10.09 532 199 228 29 555.45 114.01 533 199 229 30 555.43 114.02 534 200 226 26 555.62 114.03 535 200 227 27 555.52 114.00 536 200 228 28 555.40 114.03 537 200 264 64 96.45 0.00 538 201 226 25 555.63 114.04 539 201 227 26 555.58 114.03 540 202 225 23 555.70 114.05 541 202 226 24 555.54 114.09 542 202 264 62 93.94 1.01 543 203 224 21 555.73 114.04 544 203 225 22 555.73 114.04 545 203 227 24 555.71 114.05 546 204 222 18 556.18 113.95 547 204 223 19 556.06 113.95 548 204 224 20 555.73 114.04 549 204 235 31 513.02 135.31 550 205 222 17 556.18 113.95 551 205 223 18 556.14 113.97 552 206 221 15 556.23 113.97 553 206 222 16 556.19 113.97 554 206 250 44 117.18 1.93 555 206 251 45 117.16 1.94 556 207 220 13 556.29 113.95 557 207 221 14 556.26 113.95 558 207 250 43 117.17 1.94 559 208 219 11 556.31 113.94 560 208 220 12 556.30 113.95 561 208 241 33 512.26 135.57 562 208 244 36 452.04 117.66 563 208 247 39 121.66 3.54 564 209 218 9 556.44 113.92 565 209 219 10 556.41 113.91 566 210 218 8 556.59 113.95 567 211 217 6 556.72 113.92 568 211 218 7 556.63 113.93 569 211 241 30 512.26 135.51 570 211 242 31 511.61 135.47 571 211 243 32 506.63 133.15 572 211 244 33 506.62 133.14 573 212 216 4 756.63 14.00 574 212 217 5 556.70 113.92 575 212 218 6 556.67 113.92 576 213 216 3 791.88 0.12 577 217 240 23 512.50 135.58 578 217 241 24 512.44 135.59 579 217 242 25 512.23 135.60 580 218 240 22 512.47 135.57 581 218 241 23 512.41 135.59 582 219 238 19 512.52 135.56 583 219 239 20 512.49 135.57 584 219 240 21 512.38 135.61 585 219 241 22 512.28 135.59 586 220 238 18 512.48 135.59 587 220 239 19 512.42 135.59 588 220 241 21 511.89 135.59 589 220 247 27 117.15 1.94 590 220 250 30 116.05 1.56 591 221 237 16 512.48 135.59 592 221 238 17 512.35 135.60 593 221 250 29 116.09 1.56 594 222 235 13 512.94 135.37 595 222 236 14 512.62 135.50 596 222 237 15 512.46 135.55 597 222 250 28 116.98 1.98 598 222 251 29 116.09 1.55 599 222 254 32 113.35 0.26 600 222 255 33 113.34 0.25 601 222 258 36 106.33 0.69 602 223 235 12 512.93 135.36 603 223 236 13 512.51 135.53 604 224 234 10 513.02 135.31 605 224 235 11 512.93 135.32 606 225 232 7 513.16 135.25 607 225 233 8 513.12 135.27 608 225 234 9 512.98 135.29 609 225 235 10 512.97 135.30 610 226 232 6 513.14 135.25 611 226 233 7 513.11 135.25 612 226 235 9 513.08 135.24 613 226 258 32 112.56 0.50 614 226 263 37 95.70 0.12 615 226 264 38 92.20 0.28 616 227 231 4 752.88 17.05 617 227 232 5 556.42 113.58 618 228 231 3 788.89 4.47 619 228 233 5 751.56 16.39 620 228 262 34 105.13 0.70 621 228 263 35 102.91 1.04 622 233 263 30 94.11 0.67 623 235 258 23 104.42 0.74 624 237 250 13 116.09 1.55 625 237 253 16 113.55 0.34 626 237 254 17 112.58 0.51 627 239 246 7 705.59 39.42 628 239 249 10 116.14 1.61 629 239 250 11 115.95 1.52 630 241 244 3 920.19 0.84 631 241 246 5 743.74 20.50 632 241 247 6 707.72 38.36 633 243 246 3 841.62 2.09 634 244 247 3 840.96 2.42 635 245 248 3 901.75 5.37 636 246 249 3 841.70 2.04 637 247 250 3 839.98 1.93 638 248 251 3 831.06 4.25 639 248 252 4 788.38 4.40 640 249 252 3 823.80 1.17 641 249 253 4 788.27 4.34 642 249 254 5 790.56 5.49 643 250 254 4 795.39 7.05 644 251 254 3 826.72 0.51 645 251 255 4 788.07 4.25 646 252 255 3 797.62 1.86 647 252 256 4 787.31 4.67 648 253 256 3 811.70 4.43 649 253 257 4 785.30 5.68 650 254 257 3 810.05 2.86 651 254 258 4 785.30 5.68 652 255 258 3 806.29 1.01 653 255 259 4 787.72 8.68 654 255 264 9 90.30 0.69 655 256 259 3 814.25 4.58 656 256 260 4 791.72 6.67 657 257 260 3 806.70 0.81 658 257 261 4 790.08 7.50 659 258 261 3 793.15 5.96 660 258 262 4 790.08 7.50 661 258 263 5 650.04 18.57 662 258 264 6 614.18 36.50 663 259 262 3 791.00 7.96 664 259 264 5 614.16 36.51