BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 114 112 27.37 1.44 2 2 136 134 27.33 1.46 3 2 137 135 27.21 1.46 4 2 141 139 27.50 1.44 5 3 134 131 27.61 1.39 6 3 135 132 27.61 1.39 7 3 136 133 27.61 1.39 8 3 137 134 27.61 1.39 9 3 138 135 27.61 1.39 10 3 168 165 27.64 1.40 11 5 9 4 809.97 3.21 12 5 10 5 771.05 7.71 13 5 13 8 771.07 7.71 14 5 135 130 39.80 1.41 15 5 136 131 39.80 1.41 16 6 9 3 862.07 6.48 17 6 10 4 797.75 0.88 18 6 185 179 47.05 1.11 19 7 10 3 838.70 2.77 20 7 11 4 821.64 1.01 21 8 11 3 834.24 5.00 22 8 12 4 814.91 0.75 23 8 57 49 49.12 0.36 24 8 88 80 49.12 0.36 25 8 89 81 49.16 0.35 26 8 112 104 50.09 0.61 27 8 184 176 49.20 0.33 28 8 185 177 49.22 0.32 29 8 187 179 49.19 0.33 30 9 12 3 845.32 0.54 31 9 13 4 824.72 0.32 32 9 112 103 49.93 0.65 33 9 135 126 50.20 0.56 34 9 136 127 50.22 0.56 35 9 184 175 49.22 0.32 36 9 185 176 49.23 0.32 37 10 13 3 829.69 0.59 38 10 14 4 818.74 3.31 39 11 14 3 824.88 1.76 40 11 15 4 823.69 0.84 41 11 87 76 52.84 0.98 42 11 88 77 52.54 0.85 43 12 15 3 835.90 5.26 44 12 16 4 823.69 0.84 45 12 85 73 52.54 0.85 46 12 88 76 52.55 0.85 47 12 112 100 52.50 0.89 48 13 16 3 837.31 4.55 49 13 17 4 823.38 1.00 50 13 114 101 59.83 0.47 51 13 136 123 53.84 0.66 52 14 17 3 829.47 0.62 53 14 18 4 823.16 1.10 54 15 18 3 852.39 1.77 55 15 19 4 816.26 2.35 56 15 85 70 62.93 0.92 57 15 87 72 63.11 0.83 58 16 19 3 824.11 1.57 59 16 112 96 63.38 1.00 60 16 114 98 71.34 0.82 61 16 115 99 75.45 0.39 62 16 118 102 70.69 1.02 63 17 114 97 75.02 0.20 64 17 115 98 77.41 0.11 65 18 115 97 83.58 1.48 66 19 84 65 80.30 1.23 67 19 85 66 80.25 1.25 68 19 86 67 80.31 1.22 69 19 93 74 83.36 0.83 70 19 111 92 81.22 1.68 71 19 115 96 84.17 1.23 72 21 117 96 84.42 1.29 73 21 121 100 84.70 1.15 74 22 93 71 85.54 0.71 75 22 94 72 86.38 0.30 76 22 97 75 91.00 0.18 77 22 121 99 85.56 0.72 78 22 125 103 87.09 0.03 79 24 97 73 122.07 1.67 80 24 124 100 131.43 0.39 81 24 125 101 122.25 1.76 82 25 124 99 138.26 1.38 83 26 29 3 950.15 3.80 84 26 30 4 899.69 1.59 85 26 123 97 140.62 0.40 86 26 124 98 140.41 0.34 87 26 126 100 138.38 1.34 88 26 127 101 140.49 0.31 89 26 128 102 141.55 0.24 90 27 101 74 141.43 0.30 91 27 126 99 141.48 0.30 92 28 100 72 141.84 0.15 93 28 103 75 142.76 0.29 94 28 104 76 144.26 0.46 95 28 105 77 149.91 0.17 96 28 107 79 150.40 0.34 97 28 126 98 143.13 0.48 98 28 128 100 152.10 1.04 99 29 128 99 152.12 1.04 100 30 123 93 155.80 0.41 101 30 127 97 152.12 1.04 102 30 128 98 152.11 1.04 103 30 153 123 157.27 0.87 104 30 154 124 157.25 0.88 105 31 104 73 154.50 0.15 106 31 106 75 152.15 1.02 107 31 128 97 156.78 0.70 108 32 35 3 835.54 0.26 109 32 36 4 809.05 3.38 110 32 106 74 157.07 0.82 111 33 36 3 826.90 4.58 112 33 37 4 811.12 4.30 113 33 49 16 157.19 0.84 114 33 78 45 157.19 0.84 115 33 79 46 157.19 0.84 116 33 106 73 157.19 0.84 117 33 108 75 157.25 0.87 118 33 129 96 157.29 0.86 119 33 176 143 170.25 0.15 120 34 37 3 818.74 0.49 121 34 38 4 811.94 3.90 122 34 155 121 170.30 0.13 123 34 176 142 170.10 0.13 124 34 206 172 170.30 0.18 125 35 38 3 826.85 3.94 126 35 39 4 809.91 2.87 127 36 39 3 812.07 3.95 128 36 40 4 811.02 3.42 129 36 77 41 170.58 0.23 130 36 78 42 170.53 0.21 131 36 105 69 170.53 0.21 132 36 106 70 170.53 0.21 133 37 40 3 823.90 0.02 134 37 41 4 821.91 0.97 135 37 47 10 170.65 0.19 136 37 49 12 170.63 0.18 137 37 78 41 170.62 0.21 138 37 176 139 170.94 0.16 139 37 206 169 170.93 0.16 140 37 208 171 170.72 0.21 141 37 228 191 170.72 0.21 142 37 229 192 170.66 0.19 143 38 41 3 823.93 0.01 144 38 42 4 821.93 0.98 145 38 206 168 170.93 0.17 146 39 42 3 829.96 0.01 147 39 43 4 820.93 1.48 148 40 43 3 820.94 1.49 149 40 44 4 809.62 4.10 150 40 45 5 755.07 15.04 151 40 46 6 685.15 50.00 152 40 47 7 378.13 103.50 153 40 78 38 170.78 0.18 154 41 44 3 815.53 1.15 155 41 223 182 170.94 0.15 156 41 228 187 170.94 0.15 157 46 219 173 229.19 0.41 158 46 230 184 229.98 0.62 159 46 246 200 118.25 0.08 160 46 247 201 104.97 0.21 161 47 78 31 618.32 23.80 162 47 228 181 230.56 0.49 163 47 229 182 230.00 0.62 164 47 230 183 229.99 0.62 165 48 77 29 618.68 23.94 166 48 78 30 618.58 23.91 167 48 79 31 618.57 23.91 168 48 219 171 230.15 0.64 169 48 230 182 230.06 0.59 170 48 232 184 229.97 0.62 171 48 236 188 204.85 0.04 172 48 243 195 119.53 0.27 173 48 246 198 116.74 0.62 174 48 247 199 104.98 0.21 175 49 78 29 618.76 23.98 176 49 79 30 618.59 23.92 177 49 80 31 618.62 23.95 178 49 81 32 618.55 23.91 179 49 208 159 237.64 2.19 180 49 229 180 231.64 0.79 181 49 230 181 230.18 0.64 182 49 231 182 230.06 0.59 183 49 232 183 230.06 0.59 184 50 69 19 619.08 24.09 185 50 73 23 618.81 23.98 186 50 77 27 618.81 23.96 187 50 79 29 618.76 23.98 188 50 80 30 618.72 23.99 189 50 81 31 618.73 24.00 190 50 232 182 230.16 0.64 191 50 236 186 207.67 1.35 192 51 69 18 619.12 24.08 193 51 81 30 618.74 24.00 194 51 83 32 619.00 24.13 195 51 110 59 578.37 43.84 196 51 231 180 231.71 0.75 197 51 232 181 230.62 0.85 198 51 233 182 229.97 0.62 199 51 237 186 207.67 1.36 200 52 64 12 619.28 24.05 201 52 66 14 619.12 24.08 202 52 69 17 619.12 24.08 203 52 81 29 619.02 24.11 204 52 82 30 619.00 24.13 205 52 83 31 619.00 24.14 206 52 95 43 580.33 43.48 207 52 237 185 207.87 1.26 208 53 56 3 892.06 1.53 209 53 83 30 619.02 24.12 210 53 89 36 583.44 42.40 211 53 233 180 231.57 0.72 212 53 234 181 231.49 0.74 213 53 237 184 228.81 0.47 214 54 61 7 619.53 24.15 215 54 62 8 619.47 24.13 216 54 90 36 583.45 42.41 217 54 237 183 230.06 0.59 218 55 61 6 619.56 24.16 219 55 88 33 613.26 27.59 220 55 89 34 583.50 42.41 221 55 90 35 583.50 42.41 222 56 59 3 836.26 0.39 223 56 88 32 617.94 25.25 224 56 89 33 590.16 39.13 225 57 88 31 618.01 25.25 226 57 89 32 617.97 25.27 227 57 187 130 273.62 19.79 228 58 182 124 279.97 22.14 229 61 238 177 230.64 0.44 230 62 237 175 230.22 0.65 231 62 238 176 230.14 0.62 232 64 69 5 795.59 3.00 233 64 237 173 230.02 0.67 234 64 238 174 229.90 0.64 235 64 240 176 228.85 0.42 236 65 68 3 833.18 1.38 237 65 69 4 810.98 4.69 238 66 69 3 833.18 1.37 239 66 70 4 827.28 3.48 240 66 95 29 582.12 44.34 241 66 99 33 582.03 44.39 242 67 70 3 832.34 0.96 243 67 71 4 816.54 1.89 244 67 98 31 582.04 44.39 245 67 99 32 582.03 44.39 246 67 102 35 581.88 44.58 247 68 71 3 828.50 3.71 248 68 72 4 821.18 4.66 249 69 72 3 833.46 1.46 250 69 73 4 821.20 4.65 251 69 80 11 620.95 24.93 252 69 236 167 229.89 0.64 253 69 237 168 228.59 0.39 254 69 240 171 119.89 0.29 255 70 73 3 822.12 4.20 256 70 74 4 822.12 4.20 257 70 80 10 620.93 24.94 258 70 99 29 581.99 44.41 259 70 103 33 581.77 44.77 260 71 74 3 835.02 2.50 261 71 75 4 832.12 1.05 262 71 102 31 581.95 44.70 263 71 103 32 581.78 44.77 264 72 75 3 852.10 3.22 265 72 76 4 838.78 4.37 266 72 240 168 119.89 0.29 267 72 241 169 119.88 0.29 268 72 243 171 119.48 0.28 269 72 244 172 119.43 0.25 270 73 76 3 838.78 4.37 271 73 77 4 805.66 5.07 272 73 80 7 621.20 25.05 273 73 105 32 599.37 53.59 274 73 236 163 228.58 0.38 275 73 240 167 119.89 0.29 276 73 243 170 119.44 0.26 277 74 103 29 597.07 52.41 278 74 104 30 606.23 57.00 279 74 105 31 599.37 53.59 280 76 243 167 119.49 0.28 281 76 244 168 119.37 0.24 282 76 247 171 105.33 0.18 283 77 105 28 629.10 68.55 284 77 243 166 119.44 0.26 285 78 106 28 629.07 68.56 286 79 105 26 629.08 68.56 287 79 106 27 629.03 68.59 288 80 99 19 629.52 68.41 289 80 103 23 629.27 68.52 290 80 105 25 629.14 68.56 291 80 106 26 629.10 68.58 292 80 107 27 628.96 68.59 293 80 108 28 628.58 68.78 294 81 108 27 628.61 68.80 295 81 110 29 628.32 68.89 296 81 231 150 231.55 0.71 297 82 96 14 629.77 68.30 298 82 99 17 629.75 68.30 299 82 107 25 629.12 68.59 300 82 108 26 629.08 68.59 301 82 109 27 628.65 68.76 302 82 110 28 628.45 68.84 303 83 89 6 632.91 67.14 304 83 91 8 632.22 67.34 305 83 92 9 630.25 68.32 306 83 110 27 628.50 68.83 307 84 91 7 632.23 67.34 308 84 92 8 630.25 68.32 309 84 93 9 630.25 68.32 310 84 96 12 630.02 68.31 311 84 109 25 629.00 68.65 312 84 110 26 628.68 68.79 313 84 111 27 628.59 68.82 314 85 88 3 875.95 1.24 315 85 89 4 755.18 6.07 316 85 90 5 632.99 67.14 317 85 91 6 632.74 67.24 318 85 111 26 628.63 68.81 319 85 112 27 628.61 68.83 320 86 90 4 632.99 67.14 321 86 91 5 632.96 67.16 322 86 92 6 632.96 67.16 323 89 112 23 628.64 68.84 324 92 95 3 845.30 2.62 325 92 96 4 820.98 0.97 326 93 96 3 839.98 2.33 327 93 97 4 817.15 0.93 328 93 109 16 629.17 68.58 329 93 118 25 507.55 34.59 330 93 121 28 495.22 40.63 331 93 125 32 495.09 40.64 332 94 97 3 822.71 1.84 333 94 98 4 822.66 1.82 334 95 98 3 822.71 1.84 335 95 99 4 822.64 1.83 336 96 99 3 822.62 1.84 337 96 100 4 822.50 1.91 338 96 107 11 629.31 68.57 339 96 109 13 629.12 68.59 340 96 125 29 495.00 40.65 341 96 127 31 494.78 40.61 342 97 100 3 831.69 6.50 343 97 101 4 822.48 1.90 344 97 125 28 495.00 40.65 345 98 101 3 825.09 9.80 346 98 102 4 824.81 9.94 347 99 102 3 835.14 4.78 348 99 103 4 810.15 2.63 349 99 107 8 629.32 68.57 350 100 103 3 850.65 0.14 351 100 107 7 629.32 68.56 352 100 125 25 494.99 40.65 353 100 126 26 494.78 40.59 354 103 107 4 867.04 1.37 355 106 128 22 494.62 40.61 356 107 127 20 494.75 40.60 357 107 128 21 494.69 40.58 358 107 129 22 494.65 40.60 359 108 129 21 494.63 40.61 360 108 130 22 494.51 40.64 361 108 131 23 494.51 40.64 362 108 157 49 362.55 62.45 363 108 231 123 231.54 0.70 364 109 113 4 888.18 0.81 365 109 118 9 495.26 40.66 366 109 122 13 494.81 40.58 367 109 127 18 494.81 40.62 368 109 129 20 494.71 40.62 369 109 130 21 494.61 40.59 370 109 131 22 494.51 40.65 371 110 131 21 494.49 40.64 372 110 210 100 251.97 7.92 373 110 231 121 234.19 0.70 374 111 131 20 494.66 40.62 375 111 133 22 494.49 40.64 376 112 118 6 774.65 2.00 377 112 133 21 494.47 40.65 378 112 136 24 480.95 40.17 379 112 184 72 281.93 20.95 380 113 118 5 773.54 2.56 381 113 119 6 774.65 2.00 382 113 130 17 494.81 40.66 383 113 131 18 494.72 40.62 384 113 132 19 494.66 40.62 385 113 137 24 479.06 43.14 386 113 145 32 414.35 38.09 387 114 118 4 829.58 1.39 388 114 136 22 494.20 40.66 389 114 137 23 483.17 42.40 390 114 145 31 414.44 38.05 391 115 118 3 850.48 1.78 392 115 145 30 414.45 38.04 393 116 119 3 844.54 0.77 394 116 120 4 815.00 4.18 395 116 145 29 414.40 38.06 396 116 148 32 414.31 38.04 397 117 120 3 827.18 0.19 398 117 121 4 819.51 6.43 399 118 121 3 826.89 2.75 400 118 122 4 814.23 9.08 401 119 122 3 826.90 2.74 402 119 123 4 818.06 7.16 403 119 130 11 494.93 40.66 404 119 145 26 414.20 38.07 405 119 148 29 414.14 38.06 406 119 149 30 414.00 38.09 407 119 152 33 413.83 37.95 408 119 154 35 413.48 37.84 409 120 123 3 822.60 4.89 410 120 124 4 814.21 9.09 411 120 148 28 414.25 38.04 412 120 152 32 413.76 37.98 413 121 124 3 830.25 0.40 414 121 125 4 798.48 0.76 415 122 125 3 833.18 1.85 416 122 126 4 795.71 4.03 417 122 127 5 691.40 39.49 418 122 130 8 494.97 40.67 419 122 154 32 413.25 37.80 420 123 126 3 831.81 2.54 421 123 152 29 413.36 37.83 422 123 154 31 413.20 37.77 423 127 154 27 402.96 42.58 424 128 154 26 366.01 60.98 425 129 154 25 366.02 60.98 426 129 155 26 366.00 60.98 427 129 156 27 363.61 61.94 428 130 149 19 407.24 40.56 429 130 154 24 381.25 53.51 430 130 156 26 363.23 62.24 431 130 157 27 362.76 62.36 432 131 157 26 362.77 62.36 433 131 159 28 362.73 62.36 434 132 137 5 802.15 7.53 435 132 142 10 414.06 38.09 436 132 145 13 414.03 38.08 437 132 146 14 412.95 38.62 438 132 156 24 366.05 61.01 439 132 157 25 365.30 61.28 440 132 158 26 362.75 62.37 441 132 159 27 362.74 62.36 442 133 136 3 858.52 3.07 443 133 137 4 818.89 0.84 444 133 159 26 362.78 62.34 445 133 184 51 282.47 21.04 446 134 137 3 839.77 1.97 447 134 142 8 414.56 38.09 448 134 158 24 365.39 61.22 449 134 159 25 364.75 61.48 450 134 160 26 364.52 61.52 451 134 165 31 317.72 37.56 452 134 168 34 336.76 28.03 453 134 169 35 336.76 28.03 454 135 161 26 364.75 61.36 455 135 165 30 317.89 37.93 456 135 183 48 304.63 31.37 457 137 141 4 795.82 8.89 458 137 142 5 791.94 7.08 459 137 145 8 679.89 48.99 460 138 141 3 831.51 5.76 461 138 142 4 823.85 9.59 462 139 142 3 836.61 3.21 463 139 143 4 809.34 2.16 464 139 168 29 392.11 26.75 465 139 171 32 367.45 14.01 466 139 172 33 366.03 13.30 467 140 143 3 850.35 3.66 468 140 144 4 822.88 4.60 469 141 144 3 830.85 1.58 470 141 145 4 822.88 4.61 471 142 145 3 828.33 3.71 472 142 146 4 801.80 5.94 473 142 168 26 392.58 26.51 474 142 172 30 362.43 11.37 475 143 146 3 823.85 5.08 476 143 147 4 819.64 2.98 477 143 172 29 362.41 11.36 478 144 147 3 822.49 1.70 479 144 148 4 806.14 0.70 480 145 148 3 816.68 4.56 481 145 149 4 811.02 3.14 482 146 149 3 840.37 1.29 483 146 150 4 803.00 1.67 484 146 156 10 414.09 37.18 485 146 158 12 413.94 37.15 486 146 172 26 361.53 10.88 487 146 174 28 361.05 10.66 488 147 150 3 829.58 4.11 489 147 151 4 808.49 4.41 490 148 151 3 829.28 4.20 491 148 152 4 826.95 4.82 492 149 152 3 844.84 0.93 493 149 153 4 826.93 4.82 494 149 154 5 784.57 10.54 495 149 156 7 603.11 56.88 496 150 153 3 799.00 4.96 497 150 154 4 796.46 4.74 498 150 155 5 784.70 10.61 499 150 156 6 731.59 15.94 500 150 173 23 529.99 73.42 501 150 174 24 515.63 66.38 502 150 175 25 515.36 66.52 503 155 175 20 538.78 78.64 504 155 176 21 538.74 78.64 505 156 176 20 538.75 78.66 506 157 176 19 538.72 78.67 507 157 177 20 538.76 78.72 508 157 178 21 538.67 78.70 509 157 208 51 484.57 105.66 510 157 231 74 244.56 5.87 511 158 169 11 540.74 77.81 512 158 172 14 539.82 78.27 513 158 174 16 538.90 78.68 514 158 176 18 538.85 78.70 515 158 177 19 538.78 78.74 516 158 178 20 538.77 78.74 517 159 178 19 538.75 78.75 518 159 180 21 539.35 79.19 519 159 184 25 524.00 87.06 520 159 210 51 484.31 105.80 521 160 165 5 545.46 76.31 522 160 166 6 545.28 76.20 523 160 169 9 545.42 76.35 524 160 177 17 539.49 79.06 525 160 178 18 538.91 78.81 526 160 179 19 539.27 79.22 527 160 180 20 539.39 79.34 528 160 197 37 505.45 96.14 529 160 201 41 504.74 95.96 530 161 165 4 805.56 3.31 531 161 180 19 539.38 79.35 532 161 182 21 539.41 79.40 533 161 183 22 533.86 82.17 534 161 193 32 505.75 96.20 535 161 197 36 505.67 96.18 536 162 165 3 819.27 0.32 537 162 166 4 813.29 3.31 538 162 197 35 505.70 96.19 539 163 166 3 819.26 0.32 540 163 167 4 811.15 0.98 541 163 196 33 505.80 96.17 542 163 197 34 505.74 96.17 543 163 200 37 505.63 96.22 544 164 167 3 819.25 0.33 545 164 168 4 820.03 1.88 546 165 168 3 820.05 1.87 547 165 169 4 820.04 1.88 548 166 169 3 820.04 1.88 549 166 170 4 820.33 1.73 550 166 177 11 539.80 79.22 551 166 197 31 505.65 96.23 552 166 200 34 505.60 96.23 553 166 201 35 505.51 96.27 554 167 170 3 830.97 1.42 555 167 171 4 811.89 2.49 556 168 171 3 836.93 8.07 557 168 172 4 810.00 1.55 558 169 172 3 841.12 6.67 559 169 173 4 818.52 3.14 560 169 174 5 562.87 73.39 561 169 177 8 539.96 79.27 562 170 173 3 826.30 0.74 563 170 177 7 540.31 79.24 564 170 202 32 505.38 96.34 565 174 177 3 880.63 4.76 566 174 205 31 486.25 105.67 567 175 205 30 486.13 105.69 568 175 206 31 486.12 105.69 569 176 205 29 486.05 105.73 570 176 206 30 485.48 105.94 571 176 208 32 485.44 105.92 572 177 201 24 503.86 97.13 573 177 205 28 486.04 105.73 574 177 206 29 485.80 105.77 575 177 207 30 484.84 105.78 576 177 208 31 484.82 105.77 577 178 208 30 484.69 105.71 578 178 210 32 484.53 105.69 579 179 194 15 505.76 96.33 580 179 197 18 505.75 96.32 581 179 198 19 505.73 96.32 582 179 201 22 505.55 96.40 583 179 207 28 484.97 105.72 584 179 208 29 484.79 105.76 585 179 209 30 484.44 105.82 586 179 210 31 484.35 105.79 587 180 184 4 852.90 4.74 588 180 210 30 483.57 106.20 589 180 211 31 483.53 106.18 590 181 188 7 507.20 96.62 591 181 189 8 506.10 96.31 592 181 190 9 506.06 96.33 593 181 191 10 506.03 96.33 594 181 194 13 505.83 96.34 595 181 210 29 483.64 106.24 596 181 211 30 483.56 106.22 597 182 187 5 507.31 96.58 598 182 188 6 507.06 96.69 599 182 189 7 506.11 96.31 600 182 211 29 481.89 107.05 601 183 186 3 840.60 1.60 602 183 187 4 510.51 94.98 603 183 188 5 507.30 96.58 604 184 187 3 836.01 3.54 605 189 212 23 481.89 107.11 606 190 193 3 839.50 5.21 607 190 194 4 821.53 3.47 608 191 194 3 821.53 3.47 609 191 195 4 821.53 3.47 610 191 212 21 483.91 106.13 611 191 218 27 449.32 91.07 612 192 195 3 827.26 3.70 613 192 196 4 820.75 5.75 614 193 196 3 829.80 1.22 615 193 197 4 810.52 0.05 616 194 197 3 829.80 1.22 617 194 198 4 821.69 3.43 618 194 209 15 484.81 105.78 619 194 210 16 484.80 105.78 620 195 198 3 830.93 1.18 621 195 199 4 825.73 1.42 622 195 221 26 255.60 2.36 623 195 222 27 254.31 2.23 624 195 225 30 246.28 6.24 625 196 199 3 839.36 5.40 626 196 200 4 827.47 0.55 627 197 200 3 852.55 1.20 628 197 201 4 827.46 0.55 629 198 201 3 852.55 1.20 630 198 207 9 485.07 105.74 631 198 222 24 254.28 2.22 632 198 225 27 246.12 6.22 633 198 227 29 245.98 6.16 634 201 205 4 825.91 0.19 635 201 207 6 541.29 92.51 636 202 205 3 841.02 2.00 637 202 207 5 825.86 0.16 638 203 207 4 844.12 1.46 639 206 228 22 245.66 6.21 640 207 226 19 245.99 6.15 641 207 227 20 245.80 6.24 642 207 228 21 245.71 6.20 643 207 229 22 245.65 6.21 644 208 227 19 245.83 6.22 645 208 229 21 245.54 6.16 646 208 230 22 245.16 5.97 647 208 231 23 244.91 5.87 648 209 213 4 855.63 0.87 649 209 222 13 246.03 6.16 650 209 227 18 245.99 6.15 651 209 229 20 245.62 6.12 652 209 230 21 245.09 5.92 653 209 231 22 244.77 5.77 654 210 231 21 244.77 5.77 655 211 231 20 244.97 5.82 656 212 218 6 813.37 4.32 657 213 218 5 779.85 2.89 658 213 219 6 770.46 1.05 659 213 222 9 591.75 81.75 660 213 230 17 245.56 5.91 661 213 232 19 244.85 5.75 662 213 235 22 244.62 5.70 663 214 218 4 831.87 4.84 664 214 246 32 119.59 0.33 665 215 218 3 845.23 1.83 666 215 219 4 824.44 1.02 667 215 246 31 119.53 0.31 668 216 219 3 834.01 3.77 669 216 220 4 822.27 0.04 670 216 245 29 119.67 0.24 671 216 246 30 119.37 0.29 672 216 248 32 119.27 0.29 673 217 220 3 827.21 0.36 674 217 221 4 822.28 0.06 675 218 221 3 834.56 3.31 676 218 222 4 834.11 3.29 677 219 222 3 835.45 2.62 678 219 223 4 822.83 2.35 679 219 230 11 246.09 6.11 680 219 232 13 246.09 6.11 681 219 246 27 119.27 0.29 682 220 223 3 835.38 2.56 683 220 224 4 824.21 3.02 684 221 224 3 838.33 1.09 685 221 225 4 824.67 2.79 686 222 225 3 828.57 0.84 687 222 226 4 824.66 2.79 688 222 227 5 787.32 3.39 689 222 230 8 568.19 82.58 690 223 226 3 826.71 3.81 691 223 227 4 806.00 5.95 692 223 228 5 782.03 6.04 693 223 230 7 625.77 64.94 694 227 230 3 922.26 0.38 695 232 235 3 890.70 1.92 696 232 236 4 830.37 4.50 697 232 246 14 118.91 0.46 698 233 236 3 849.31 2.73 699 233 237 4 817.22 2.08 700 234 237 3 830.99 4.18 701 234 238 4 817.23 2.08 702 235 238 3 831.62 5.12 703 235 239 4 807.07 3.00 704 236 239 3 813.05 9.09 705 236 240 4 608.37 58.29 706 236 242 6 479.66 122.63 707 236 243 7 470.63 118.13 708 236 246 10 413.42 146.74 709 239 242 3 845.31 5.37 710 240 243 3 858.94 1.44 711 241 244 3 847.98 1.71 712 242 245 3 839.17 6.11 713 242 246 4 820.65 2.17 714 243 246 3 822.65 1.17 715 243 247 4 820.65 2.17 716 244 247 3 851.75 5.06 717 244 248 4 844.41 8.72 718 245 248 3 850.02 4.20