BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 114 112 41.75 5.26 2 2 136 134 44.62 6.67 3 2 137 135 44.56 6.68 4 2 138 136 45.40 7.06 5 2 141 139 45.40 7.06 6 3 134 131 45.42 7.07 7 3 135 132 45.62 7.17 8 3 136 133 45.40 7.06 9 3 137 134 45.41 7.07 10 3 138 135 45.41 7.07 11 3 165 162 46.16 7.38 12 5 9 4 792.57 3.70 13 5 10 5 635.84 22.87 14 5 13 8 593.78 41.05 15 5 135 130 49.51 7.70 16 5 136 131 49.46 7.67 17 6 9 3 861.00 4.09 18 6 10 4 801.19 0.27 19 7 10 3 840.58 1.90 20 7 11 4 829.35 1.30 21 8 11 3 834.53 1.13 22 8 12 4 826.23 0.24 23 8 57 49 60.38 5.42 24 8 88 80 60.38 5.41 25 8 89 81 61.54 5.99 26 8 112 104 61.92 6.17 27 8 184 176 61.94 6.19 28 8 185 177 61.93 6.19 29 8 187 179 61.69 6.07 30 9 12 3 832.72 0.31 31 9 13 4 813.68 4.74 32 9 112 103 61.91 6.17 33 9 135 126 62.08 6.25 34 9 136 127 62.08 6.25 35 9 184 175 61.94 6.19 36 9 185 176 61.94 6.19 37 10 13 3 827.09 1.41 38 10 14 4 825.16 0.77 39 11 14 3 827.63 0.47 40 11 15 4 825.16 0.77 41 11 87 76 64.79 4.93 42 11 88 77 63.68 5.45 43 12 15 3 822.88 2.85 44 12 16 4 813.44 5.10 45 12 85 73 63.70 5.46 46 12 88 76 63.66 5.44 47 12 112 100 63.73 5.48 48 13 16 3 822.03 3.27 49 13 17 4 823.26 0.20 50 13 114 101 74.04 5.92 51 13 136 123 69.67 6.58 52 14 17 3 825.88 1.13 53 14 18 4 823.26 0.18 54 15 18 3 851.96 0.35 55 15 19 4 820.66 1.11 56 15 85 70 73.17 3.97 57 15 87 72 72.89 4.09 58 16 19 3 812.41 5.24 59 16 112 96 76.16 5.48 60 16 114 98 82.95 6.50 61 16 115 99 84.56 5.84 62 16 118 102 83.80 6.09 63 17 114 97 84.72 5.75 64 17 115 98 85.39 5.46 65 18 115 97 87.06 5.74 66 19 84 65 86.35 5.36 67 19 85 66 86.33 5.36 68 19 86 67 86.33 5.35 69 19 92 73 86.36 5.35 70 19 93 74 86.38 5.35 71 19 111 92 86.34 5.36 72 19 115 96 87.09 5.73 73 20 93 73 87.12 5.65 74 21 117 96 93.23 2.74 75 21 121 100 87.23 5.72 76 22 93 71 87.25 5.70 77 22 121 99 87.27 5.71 78 22 124 102 93.57 2.56 79 22 125 103 87.27 5.71 80 24 124 100 129.74 0.12 81 26 29 3 953.39 2.46 82 26 30 4 892.02 0.10 83 26 123 97 150.41 6.25 84 26 124 98 137.81 0.12 85 26 126 100 137.94 0.18 86 26 127 101 151.44 6.61 87 26 128 102 152.29 6.21 88 27 101 74 151.41 6.66 89 27 126 99 151.50 6.66 90 28 100 72 151.50 6.66 91 28 103 75 153.40 6.76 92 28 104 76 153.44 6.78 93 28 105 77 157.72 5.14 94 28 107 79 157.22 4.90 95 28 126 98 153.40 6.77 96 28 128 100 159.89 5.20 97 29 128 99 160.18 5.34 98 30 123 93 165.80 4.08 99 30 128 98 159.90 5.20 100 30 152 122 170.03 2.98 101 30 153 123 170.03 2.98 102 30 154 124 167.12 4.41 103 31 104 73 165.93 4.48 104 31 106 75 160.59 5.16 105 31 128 97 166.83 4.44 106 32 35 3 838.08 1.07 107 32 36 4 822.43 1.90 108 32 106 74 166.93 4.40 109 33 36 3 834.44 2.89 110 33 37 4 823.41 2.39 111 33 49 16 166.95 4.40 112 33 78 45 167.00 4.38 113 33 79 46 167.09 4.42 114 33 106 73 167.10 4.44 115 33 108 75 167.15 4.42 116 33 129 96 170.05 2.98 117 33 176 143 174.61 1.64 118 34 37 3 834.44 2.89 119 34 38 4 828.43 0.12 120 34 155 121 174.65 1.65 121 34 176 142 174.52 1.59 122 34 206 172 174.83 1.74 123 35 38 3 817.00 5.88 124 35 39 4 822.98 1.62 125 36 39 3 834.34 2.79 126 36 40 4 817.73 1.02 127 36 77 41 175.11 1.81 128 36 78 42 175.06 1.80 129 36 105 69 175.07 1.80 130 36 106 70 175.06 1.79 131 37 40 3 820.04 0.13 132 37 41 4 828.68 4.46 133 37 47 10 175.15 1.82 134 37 49 12 175.12 1.81 135 37 78 41 175.11 1.80 136 37 176 139 175.66 1.80 137 37 206 169 175.63 1.81 138 37 208 171 175.28 1.81 139 37 228 191 175.28 1.81 140 37 229 192 175.13 1.81 141 38 41 3 828.70 4.45 142 38 42 4 828.70 4.45 143 38 206 168 175.66 1.80 144 38 228 190 175.52 1.81 145 39 42 3 828.70 4.45 146 39 43 4 817.76 1.02 147 40 43 3 823.03 1.61 148 40 44 4 716.81 40.15 149 40 45 5 537.99 17.20 150 40 46 6 537.99 17.20 151 40 47 7 175.38 1.74 152 40 78 38 175.38 1.71 153 41 44 3 804.45 6.25 154 41 223 182 179.07 0.12 155 41 228 187 175.65 1.82 156 46 219 173 225.61 0.82 157 46 230 184 225.88 0.78 158 46 246 200 75.86 21.59 159 46 247 201 69.02 18.20 160 47 78 31 578.50 45.44 161 47 228 181 226.12 0.70 162 47 229 182 225.90 0.78 163 47 230 183 225.91 0.77 164 48 77 29 578.75 45.41 165 48 78 30 578.73 45.43 166 48 79 31 578.59 45.44 167 48 219 171 226.07 0.71 168 48 230 182 225.95 0.75 169 48 232 184 225.87 0.78 170 48 236 188 207.11 0.77 171 48 243 195 79.91 21.69 172 48 246 198 73.39 20.35 173 48 247 199 69.02 18.20 174 49 78 29 578.83 45.44 175 49 79 30 578.53 45.45 176 49 80 31 578.53 45.44 177 49 81 32 578.48 45.42 178 49 208 159 275.41 7.44 179 49 229 180 226.65 0.54 180 49 230 181 226.07 0.71 181 49 231 182 225.90 0.78 182 49 232 183 225.89 0.78 183 50 69 19 579.21 45.35 184 50 73 23 579.15 45.33 185 50 77 27 579.12 45.34 186 50 79 29 578.89 45.40 187 50 80 30 578.75 45.41 188 50 81 31 578.74 45.41 189 50 232 182 225.97 0.75 190 50 236 186 207.12 0.77 191 51 69 18 579.58 45.31 192 51 81 30 578.75 45.41 193 51 83 32 578.89 45.38 194 51 110 59 578.49 45.42 195 51 231 180 226.70 0.57 196 51 232 181 226.06 0.71 197 51 233 182 225.87 0.78 198 51 237 186 207.12 0.77 199 52 64 12 580.11 45.50 200 52 66 14 579.58 45.31 201 52 69 17 579.63 45.29 202 52 81 29 579.07 45.33 203 52 82 30 578.88 45.39 204 52 83 31 578.90 45.38 205 52 95 43 578.95 45.39 206 52 237 185 207.97 1.19 207 53 56 3 893.42 0.85 208 53 62 9 581.13 45.24 209 53 83 30 578.93 45.37 210 53 89 36 580.47 45.59 211 53 234 181 226.50 0.58 212 53 237 184 222.62 1.71 213 54 61 7 581.18 45.26 214 54 62 8 581.05 45.29 215 54 90 36 581.24 45.23 216 54 91 37 580.59 45.55 217 54 95 41 580.50 45.55 218 54 237 183 225.79 0.84 219 55 61 6 581.22 45.24 220 55 87 32 581.65 45.37 221 55 88 33 581.65 45.38 222 55 89 34 581.31 45.24 223 55 90 35 581.31 45.24 224 56 59 3 833.50 1.95 225 56 88 32 581.67 45.38 226 56 89 33 581.37 45.27 227 57 88 31 581.75 45.39 228 57 89 32 581.75 45.40 229 57 187 130 281.30 6.50 230 58 182 124 281.34 6.52 231 60 238 178 226.55 0.57 232 62 237 175 225.90 0.79 233 64 69 5 798.18 3.97 234 64 237 173 225.63 0.85 235 64 238 174 225.52 0.86 236 64 240 176 222.84 0.97 237 65 68 3 840.86 4.31 238 65 69 4 808.91 1.27 239 66 69 3 834.50 1.12 240 66 70 4 820.75 4.66 241 66 95 29 582.56 45.52 242 66 99 33 582.67 45.56 243 67 70 3 831.16 0.55 244 67 71 4 817.93 3.71 245 67 98 31 582.71 45.56 246 67 99 32 582.68 45.56 247 67 102 35 582.74 45.55 248 68 71 3 829.75 0.16 249 68 72 4 817.94 3.71 250 69 72 3 837.81 4.43 251 69 73 4 821.22 2.06 252 69 236 167 224.97 0.70 253 69 237 168 222.90 0.96 254 69 240 171 181.19 0.58 255 70 73 3 825.83 1.56 256 70 74 4 823.02 2.96 257 70 80 10 582.71 45.53 258 70 99 29 582.74 45.56 259 70 103 33 593.33 50.20 260 71 74 3 833.49 0.49 261 71 75 4 845.89 6.17 262 71 102 31 593.49 50.11 263 71 103 32 593.31 50.19 264 72 75 3 856.25 2.98 265 72 76 4 845.90 6.18 266 72 240 168 181.22 0.60 267 72 241 169 181.18 0.58 268 72 243 171 110.73 6.15 269 72 244 172 110.29 6.33 270 73 76 3 844.18 7.04 271 73 77 4 796.91 2.79 272 73 80 7 582.76 45.54 273 73 105 32 626.41 40.42 274 73 236 163 221.52 1.65 275 73 240 167 152.95 14.70 276 73 243 170 105.96 8.54 277 74 103 29 625.50 39.97 278 74 104 30 626.69 40.55 279 74 105 31 625.50 39.97 280 76 243 167 105.64 8.69 281 76 244 168 79.75 21.60 282 76 247 171 69.03 18.19 283 77 105 28 679.53 46.41 284 77 243 166 79.78 21.61 285 78 106 28 679.68 46.34 286 79 105 26 679.69 46.34 287 79 106 27 679.50 46.41 288 80 99 19 679.89 46.34 289 80 103 23 679.83 46.37 290 80 105 25 679.79 46.38 291 80 106 26 679.76 46.37 292 80 107 27 679.50 46.41 293 80 108 28 679.50 46.41 294 81 108 27 679.50 46.41 295 81 110 29 679.39 46.43 296 81 231 150 226.56 0.55 297 82 95 13 680.04 46.35 298 82 96 14 679.95 46.37 299 82 99 17 679.96 46.37 300 82 107 25 679.80 46.37 301 82 108 26 679.76 46.37 302 82 109 27 679.50 46.41 303 82 110 28 679.49 46.41 304 83 89 6 680.27 46.37 305 83 91 8 680.18 46.34 306 83 110 27 679.57 46.38 307 84 91 7 680.18 46.34 308 84 92 8 680.14 46.36 309 84 93 9 680.14 46.36 310 84 96 12 680.09 46.34 311 84 109 25 679.79 46.38 312 84 110 26 679.75 46.38 313 84 111 27 679.68 46.38 314 85 88 3 871.34 1.62 315 85 89 4 680.43 46.33 316 85 90 5 680.31 46.37 317 85 91 6 680.24 46.37 318 85 111 26 679.75 46.37 319 85 112 27 679.75 46.37 320 86 90 4 680.30 46.37 321 86 91 5 680.27 46.37 322 89 112 23 679.75 46.37 323 92 95 3 847.70 0.77 324 92 96 4 811.20 0.82 325 93 96 3 821.23 2.14 326 93 97 4 810.75 0.58 327 93 109 16 679.83 46.38 328 93 121 28 293.69 5.55 329 93 125 32 293.26 5.54 330 94 97 3 820.96 2.27 331 94 98 4 810.75 0.58 332 95 98 3 834.10 4.01 333 95 99 4 811.21 0.81 334 96 99 3 829.89 2.01 335 96 100 4 816.46 2.33 336 96 107 11 679.83 46.37 337 96 109 13 679.78 46.38 338 96 125 29 292.06 6.02 339 96 127 31 291.91 6.00 340 97 100 3 844.36 1.06 341 97 101 4 827.16 7.53 342 97 125 28 292.07 6.03 343 98 101 3 844.36 1.06 344 98 102 4 827.16 7.54 345 99 102 3 834.84 3.70 346 99 103 4 827.16 7.54 347 99 107 8 679.86 46.38 348 100 103 3 843.98 0.88 349 100 107 7 679.87 46.38 350 100 125 25 292.02 6.04 351 100 126 26 291.88 6.02 352 103 107 4 861.69 0.89 353 106 128 22 287.04 7.00 354 107 127 20 287.23 6.95 355 107 128 21 287.14 6.93 356 107 129 22 287.01 6.99 357 108 129 21 287.01 6.99 358 108 130 22 287.01 6.99 359 108 131 23 286.64 6.90 360 108 157 49 286.25 6.77 361 108 231 123 226.56 0.55 362 109 113 4 890.55 3.83 363 109 118 9 293.44 5.57 364 109 122 13 291.04 5.86 365 109 127 18 289.19 6.79 366 109 129 20 287.15 7.02 367 109 130 21 286.91 7.00 368 109 131 22 286.87 6.99 369 110 131 21 286.63 6.90 370 110 210 100 284.56 6.77 371 110 231 121 228.13 0.60 372 112 118 6 738.69 21.66 373 112 133 21 288.29 7.30 374 112 136 24 289.02 7.19 375 112 184 72 287.00 6.96 376 113 118 5 639.46 19.37 377 113 119 6 639.47 19.38 378 113 130 17 291.11 6.21 379 113 131 18 288.95 7.00 380 113 132 19 288.49 7.21 381 113 137 24 291.67 6.21 382 113 145 32 293.44 5.57 383 114 118 4 820.40 0.21 384 114 136 22 291.37 6.29 385 114 137 23 291.47 6.29 386 114 145 31 293.55 5.51 387 115 118 3 851.43 3.56 388 115 145 30 293.69 5.48 389 116 119 3 839.27 0.92 390 116 120 4 818.89 0.95 391 116 145 29 293.90 5.40 392 116 148 32 298.23 3.78 393 117 120 3 825.50 4.25 394 117 121 4 826.77 7.15 395 118 121 3 842.27 4.13 396 118 122 4 824.69 8.19 397 119 122 3 834.50 3.29 398 119 123 4 824.68 8.19 399 119 130 11 311.19 2.96 400 119 145 26 315.66 0.98 401 119 148 29 363.72 22.82 402 119 149 30 315.80 1.04 403 119 152 33 365.03 22.83 404 120 123 3 838.52 1.27 405 120 124 4 814.26 2.98 406 120 148 28 364.39 22.51 407 120 152 32 367.66 24.05 408 121 124 3 816.23 0.74 409 121 125 4 801.41 0.14 410 122 125 3 826.08 2.95 411 122 126 4 811.06 1.47 412 122 127 5 444.04 62.27 413 122 130 8 314.65 1.33 414 122 154 32 367.64 24.04 415 123 126 3 837.75 6.32 416 123 152 29 391.50 20.80 417 123 154 31 367.62 24.06 418 127 154 27 473.19 42.90 419 128 154 26 472.94 43.02 420 129 154 25 472.94 43.02 421 129 155 26 473.19 42.89 422 129 156 27 472.90 43.02 423 130 156 26 473.19 42.88 424 130 157 27 473.18 42.88 425 131 157 26 473.20 42.88 426 131 159 28 473.23 42.84 427 132 142 10 473.53 42.79 428 132 145 13 473.53 42.79 429 132 146 14 473.50 42.80 430 132 156 24 473.36 42.82 431 132 157 25 473.34 42.83 432 132 158 26 473.29 42.83 433 132 159 27 473.29 42.83 434 133 136 3 842.45 0.24 435 133 137 4 807.34 2.67 436 133 159 26 473.33 42.81 437 133 184 51 399.16 5.71 438 134 137 3 844.52 0.80 439 134 142 8 473.64 42.79 440 134 158 24 473.43 42.80 441 134 159 25 473.39 42.81 442 134 160 26 473.36 42.80 443 134 165 31 446.42 29.29 444 134 168 34 446.42 29.29 445 134 169 35 446.38 29.27 446 135 161 26 473.38 42.79 447 135 183 48 434.94 23.57 448 135 184 49 403.37 7.79 449 137 141 4 815.32 0.68 450 137 142 5 794.42 5.40 451 137 145 8 587.08 10.15 452 138 141 3 823.18 1.89 453 138 142 4 815.33 0.68 454 139 142 3 827.68 0.36 455 139 143 4 821.12 2.21 456 139 168 29 446.53 29.15 457 139 171 32 447.70 28.56 458 139 172 33 447.68 28.54 459 140 143 3 823.37 1.79 460 140 144 4 821.13 2.21 461 141 144 3 825.75 3.52 462 141 145 4 821.12 2.21 463 142 145 3 834.96 2.92 464 142 146 4 820.10 2.71 465 142 158 16 474.67 42.19 466 142 168 26 447.68 28.58 467 142 172 30 447.52 28.45 468 143 146 3 833.39 0.29 469 143 147 4 814.11 1.68 470 143 172 29 447.47 28.42 471 144 147 3 816.86 0.26 472 144 148 4 808.07 1.34 473 145 148 3 815.09 0.29 474 145 149 4 815.73 0.51 475 146 149 3 840.52 2.14 476 146 150 4 816.41 0.76 477 146 156 10 474.80 42.12 478 146 158 12 474.79 42.13 479 146 172 26 551.38 23.57 480 146 174 28 447.41 28.37 481 147 150 3 818.44 0.81 482 147 151 4 814.57 1.13 483 148 151 3 831.71 2.29 484 148 152 4 818.69 0.38 485 149 152 3 833.77 5.50 486 149 153 4 815.88 1.79 487 149 154 5 661.60 21.36 488 149 156 7 582.92 11.82 489 150 153 3 815.87 1.78 490 150 154 4 799.33 3.12 491 150 155 5 712.20 46.66 492 150 156 6 598.94 9.97 493 150 173 23 589.69 42.74 494 150 174 24 565.90 30.87 495 150 175 25 561.45 28.71 496 155 175 20 604.74 50.41 497 155 176 21 604.48 50.35 498 156 176 20 604.72 50.42 499 157 176 19 604.73 50.43 500 157 177 20 604.68 50.44 501 157 178 21 604.61 50.46 502 157 208 51 552.14 76.70 503 158 169 11 605.23 50.32 504 158 174 16 604.99 50.41 505 158 176 18 604.79 50.43 506 158 177 19 604.70 50.45 507 158 178 20 604.70 50.45 508 159 178 19 604.72 50.46 509 159 180 21 604.62 50.48 510 159 210 51 552.09 76.75 511 160 165 5 605.54 50.27 512 160 166 6 605.52 50.27 513 160 169 9 605.50 50.29 514 160 177 17 604.88 50.47 515 160 178 18 604.82 50.49 516 160 179 19 604.74 50.48 517 160 180 20 604.70 50.50 518 160 197 37 553.34 76.25 519 160 201 41 552.97 76.50 520 161 165 4 807.52 2.62 521 161 180 19 604.70 50.50 522 161 182 21 604.69 50.51 523 161 183 22 594.41 55.65 524 161 193 32 553.33 76.19 525 161 197 36 553.39 76.25 526 162 165 3 829.79 2.56 527 162 166 4 809.49 1.05 528 162 197 35 553.44 76.27 529 163 166 3 814.60 1.23 530 163 167 4 804.87 1.25 531 163 196 33 553.47 76.27 532 163 197 34 553.45 76.28 533 163 200 37 553.21 76.43 534 164 167 3 829.75 3.94 535 164 168 4 819.50 1.22 536 165 168 3 836.80 0.41 537 165 169 4 817.66 2.14 538 166 169 3 818.88 1.54 539 166 170 4 817.66 2.13 540 166 177 11 605.14 50.45 541 166 197 31 553.37 76.31 542 166 200 34 553.20 76.47 543 166 201 35 553.03 76.53 544 167 170 3 824.71 0.49 545 167 171 4 818.20 1.86 546 168 171 3 816.49 4.61 547 168 172 4 810.41 2.02 548 169 172 3 828.71 4.59 549 169 173 4 805.60 0.83 550 169 174 5 747.39 20.60 551 169 177 8 605.16 50.50 552 170 173 3 807.63 2.18 553 170 177 7 605.25 50.47 554 170 202 32 557.93 79.02 555 174 177 3 882.28 1.72 556 175 205 30 587.36 94.13 557 175 206 31 587.35 94.13 558 176 206 30 587.37 94.16 559 176 208 32 587.33 94.16 560 177 201 24 587.72 94.09 561 177 205 28 587.49 94.16 562 177 206 29 587.42 94.17 563 177 207 30 587.35 94.17 564 177 208 31 587.35 94.17 565 178 208 30 587.30 94.19 566 178 210 32 587.10 94.25 567 179 194 15 588.36 93.80 568 179 197 18 588.36 93.81 569 179 198 19 588.35 93.81 570 179 201 22 588.04 93.98 571 179 207 28 587.47 94.17 572 179 208 29 587.40 94.18 573 179 209 30 587.27 94.22 574 179 210 31 587.12 94.26 575 180 184 4 830.44 2.92 576 180 210 30 587.15 94.28 577 180 211 31 587.14 94.27 578 181 189 8 588.62 93.75 579 181 190 9 588.58 93.75 580 181 194 13 588.47 93.80 581 181 210 29 587.22 94.27 582 181 211 30 587.15 94.27 583 181 212 31 587.14 94.27 584 182 187 5 588.75 93.76 585 182 188 6 588.73 93.75 586 182 189 7 588.62 93.76 587 182 211 29 587.18 94.28 588 183 186 3 842.73 8.56 589 183 187 4 588.77 93.77 590 183 188 5 588.75 93.77 591 184 187 3 812.44 0.38 592 189 211 22 587.26 94.27 593 189 212 23 587.24 94.27 594 190 193 3 841.94 6.53 595 190 194 4 822.83 0.43 596 191 194 3 822.83 0.44 597 191 195 4 819.48 1.24 598 191 212 21 587.29 94.25 599 191 218 27 496.89 66.52 600 192 195 3 825.11 1.40 601 192 196 4 817.22 0.09 602 193 196 3 819.60 1.27 603 193 197 4 819.41 1.27 604 194 197 3 819.43 1.27 605 194 198 4 819.38 1.24 606 194 209 15 587.50 94.22 607 194 210 16 587.50 94.22 608 195 198 3 842.75 0.04 609 195 199 4 819.39 1.25 610 195 222 27 230.11 0.73 611 195 225 30 230.13 0.74 612 196 199 3 849.34 3.33 613 196 200 4 822.06 2.58 614 197 200 3 825.72 0.76 615 197 201 4 819.38 1.25 616 198 201 3 832.63 2.62 617 198 207 9 587.69 94.16 618 198 222 24 230.06 0.71 619 198 225 27 230.06 0.71 620 198 227 29 229.96 0.77 621 201 205 4 813.50 4.38 622 201 207 6 592.57 92.01 623 202 205 3 849.94 2.56 624 202 207 5 819.31 1.48 625 203 207 4 851.22 1.94 626 206 228 22 229.25 0.86 627 207 226 19 229.91 0.74 628 207 227 20 229.28 0.86 629 207 228 21 229.27 0.86 630 207 229 22 229.25 0.86 631 208 227 19 229.85 0.78 632 208 229 21 229.24 0.86 633 208 230 22 229.24 0.86 634 208 231 23 229.20 0.85 635 209 213 4 863.34 3.37 636 209 222 13 230.00 0.70 637 209 227 18 229.93 0.74 638 209 229 20 229.29 0.86 639 209 230 21 229.24 0.86 640 209 231 22 229.24 0.86 641 210 231 21 229.19 0.86 642 211 231 20 229.28 0.86 643 212 218 6 689.02 62.49 644 213 218 5 660.45 76.78 645 213 219 6 611.91 82.70 646 213 222 9 367.88 21.62 647 213 230 17 230.06 0.74 648 213 232 19 229.84 0.77 649 214 242 28 133.96 2.77 650 214 246 32 78.11 22.70 651 215 218 3 825.48 1.16 652 215 219 4 819.38 2.68 653 215 246 31 78.14 22.68 654 216 219 3 820.99 2.81 655 216 220 4 818.75 2.37 656 216 245 29 78.56 22.47 657 216 246 30 78.03 22.65 658 216 248 32 78.00 22.64 659 217 220 3 816.95 1.48 660 217 221 4 816.95 1.48 661 218 221 3 820.87 3.50 662 218 222 4 807.66 1.45 663 219 222 3 819.81 1.87 664 219 223 4 815.63 3.96 665 219 230 11 230.20 0.74 666 219 232 13 230.19 0.74 667 219 246 27 77.78 22.55 668 220 223 3 815.66 3.97 669 220 224 4 817.83 5.05 670 221 224 3 825.47 1.23 671 221 225 4 816.39 5.76 672 222 225 3 826.36 0.79 673 222 226 4 814.23 4.68 674 222 227 5 795.36 4.74 675 222 230 8 513.58 133.52 676 223 226 3 818.28 4.83 677 223 227 4 806.85 1.01 678 223 228 5 806.85 1.01 679 223 230 7 677.50 52.27 680 227 230 3 922.53 0.07 681 232 235 3 903.18 1.50 682 232 236 4 819.44 2.48 683 232 246 14 77.49 22.41 684 233 236 3 839.66 2.07 685 233 237 4 819.45 2.47 686 234 237 3 834.68 2.59 687 234 238 4 819.42 2.46 688 235 238 3 845.65 3.24 689 235 239 4 815.62 4.35 690 236 239 3 838.24 6.96 691 236 240 4 552.15 115.48 692 236 242 6 333.85 50.72 693 236 243 7 177.59 27.04 694 236 246 10 177.33 27.16 695 239 242 3 849.63 3.58 696 240 243 3 854.05 0.95 697 241 244 3 849.90 3.02 698 242 245 3 834.57 0.94 699 242 246 4 820.11 2.23 700 243 246 3 821.63 2.98 701 243 247 4 820.09 2.22 702 244 247 3 835.95 5.71 703 244 248 4 824.48 0.03 704 245 248 3 847.33 3.61