BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 7 5 765.57 10.42 2 3 6 3 870.64 6.62 3 4 7 3 904.50 1.10 4 5 8 3 859.89 2.08 5 5 9 4 861.28 1.42 6 6 10 4 861.28 1.42 7 7 10 3 866.94 2.02 8 7 11 4 861.28 1.41 9 8 11 3 876.89 2.90 10 8 12 4 866.05 0.97 11 9 12 3 873.77 1.34 12 9 13 4 866.74 0.62 13 10 13 3 868.40 0.21 14 10 14 4 866.86 0.57 15 10 94 84 583.72 1.43 16 10 95 85 583.56 1.35 17 10 98 88 583.54 1.35 18 11 14 3 867.58 0.21 19 11 15 4 852.62 7.69 20 11 98 87 583.56 1.35 21 12 15 3 879.97 3.77 22 12 16 4 847.13 4.70 23 12 72 60 587.13 1.16 24 13 16 3 861.97 2.73 25 13 17 4 861.96 2.73 26 13 72 59 587.16 1.17 27 13 73 60 586.73 1.17 28 13 95 82 584.06 1.50 29 13 99 86 583.75 1.46 30 14 17 3 861.95 2.73 31 14 18 4 861.95 2.73 32 14 98 84 583.82 1.48 33 14 99 85 583.74 1.46 34 14 102 88 583.70 1.46 35 15 18 3 865.41 1.00 36 15 19 4 860.21 1.69 37 16 19 3 874.01 3.95 38 16 20 4 859.95 1.82 39 16 69 53 587.25 1.19 40 16 72 56 587.25 1.20 41 16 73 57 587.24 1.19 42 17 20 3 879.97 0.96 43 17 21 4 865.16 0.48 44 17 73 56 587.24 1.19 45 17 75 58 586.82 1.20 46 17 76 59 586.81 1.20 47 17 77 60 586.59 1.11 48 17 99 82 583.89 1.44 49 17 103 86 583.64 1.43 50 18 21 3 879.97 0.96 51 18 22 4 879.01 5.70 52 18 102 84 583.74 1.46 53 18 105 87 583.03 1.33 54 18 106 88 580.78 1.88 55 18 109 91 578.95 1.93 56 19 22 3 884.23 3.10 57 19 23 4 881.62 4.41 58 20 23 3 884.24 3.10 59 20 24 4 881.63 4.40 60 20 65 45 587.65 1.18 61 20 69 49 587.38 1.21 62 20 77 57 586.77 1.20 63 21 24 3 881.64 4.40 64 21 25 4 881.64 4.40 65 21 77 56 586.77 1.20 66 21 103 82 583.83 1.43 67 21 106 85 580.87 1.88 68 21 107 86 580.79 1.91 69 22 25 3 884.29 3.32 70 22 26 4 852.49 1.77 71 22 106 84 582.33 1.15 72 22 109 87 579.97 1.53 73 22 110 88 578.60 1.85 74 22 113 91 577.19 1.81 75 23 26 3 880.51 4.92 76 23 27 4 845.21 3.15 77 23 65 42 588.64 1.38 78 24 27 3 881.09 0.39 79 24 28 4 858.08 4.62 80 24 58 34 589.11 1.24 81 24 61 37 588.96 1.24 82 24 62 38 588.99 1.22 83 24 65 41 588.65 1.38 84 24 194 170 268.69 2.82 85 25 28 3 874.45 2.45 86 25 29 4 854.13 2.65 87 25 30 5 846.00 2.14 88 25 107 82 582.87 1.42 89 25 110 85 579.02 1.97 90 25 111 86 578.93 1.94 91 25 114 89 577.39 1.93 92 25 197 172 268.19 2.88 93 26 30 4 846.38 1.95 94 26 110 84 579.05 1.99 95 26 114 88 573.88 0.47 96 26 117 91 566.69 0.82 97 27 30 3 870.16 2.05 98 27 31 4 862.75 1.66 99 27 61 34 589.07 1.24 100 28 31 3 882.19 3.96 101 28 32 4 874.42 7.83 102 28 57 29 589.38 1.19 103 28 58 30 589.31 1.15 104 28 61 33 589.17 1.20 105 29 32 3 878.70 5.71 106 29 33 4 874.42 7.83 107 29 58 29 589.40 1.18 108 29 114 85 578.60 1.85 109 29 126 97 341.96 1.06 110 29 127 98 341.03 1.15 111 29 197 168 268.45 2.89 112 29 201 172 267.83 2.83 113 30 33 3 884.75 2.67 114 30 34 4 879.05 3.06 115 30 114 84 578.13 1.95 116 31 34 3 882.22 1.47 117 31 53 22 589.70 1.12 118 31 57 26 589.40 1.18 119 32 35 3 931.48 0.06 120 32 49 17 591.06 1.02 121 32 54 22 589.66 1.14 122 33 36 3 894.16 1.48 123 33 37 4 886.14 2.54 124 33 118 85 571.90 0.30 125 33 121 88 549.68 0.89 126 33 126 93 342.23 1.15 127 33 127 94 342.11 1.11 128 34 37 3 896.90 1.99 129 34 53 19 590.85 0.92 130 35 38 3 949.59 3.94 131 35 47 12 591.28 1.11 132 35 48 13 591.28 1.11 133 35 49 14 591.16 1.06 134 35 53 18 591.15 1.06 135 36 39 3 942.94 1.26 136 36 121 85 563.02 2.60 137 37 118 81 578.33 2.22 138 37 121 84 567.68 0.32 139 38 47 9 591.55 1.06 140 39 120 81 577.79 2.10 141 39 121 82 576.25 1.41 142 49 54 5 837.01 2.16 143 50 53 3 871.44 1.53 144 50 54 4 851.72 5.19 145 51 54 3 868.31 3.10 146 51 55 4 847.69 3.18 147 51 186 135 326.00 1.71 148 51 187 136 325.47 1.48 149 52 55 3 860.50 2.35 150 52 56 4 847.69 3.19 151 52 183 131 333.87 0.58 152 53 56 3 865.98 4.51 153 53 57 4 847.71 3.20 154 54 57 3 854.74 0.32 155 54 58 4 847.68 3.21 156 54 186 132 325.25 1.59 157 55 58 3 879.60 0.07 158 55 59 4 854.05 0.04 159 55 179 124 335.03 1.09 160 55 182 127 332.62 0.84 161 55 183 128 332.61 0.84 162 55 186 131 322.97 2.67 163 56 59 3 864.50 5.26 164 56 60 4 847.48 3.32 165 56 179 123 335.06 1.08 166 57 61 4 844.92 0.63 167 58 61 3 868.94 4.25 168 58 62 4 877.24 1.10 169 58 178 120 335.26 1.05 170 58 182 124 332.62 0.84 171 58 197 139 268.34 2.84 172 59 62 3 882.13 1.34 173 59 63 4 844.97 0.65 174 59 175 116 335.98 0.86 175 59 178 119 335.17 1.06 176 59 179 120 335.01 1.09 177 60 63 3 869.00 5.22 178 60 64 4 844.97 0.65 179 61 64 3 875.43 2.01 180 61 65 4 844.95 0.66 181 62 65 3 868.27 1.53 182 62 66 4 841.39 1.17 183 62 77 15 587.36 1.23 184 62 174 112 336.03 0.89 185 62 175 113 335.85 0.89 186 62 178 116 335.17 1.06 187 63 66 3 890.25 2.19 188 63 67 4 856.11 6.16 189 63 171 108 336.05 0.90 190 63 172 109 336.00 0.93 191 63 175 112 335.76 0.93 192 64 67 3 874.72 5.08 193 64 68 4 849.83 3.02 194 65 68 3 852.25 4.22 195 65 69 4 849.83 3.02 196 65 77 12 587.32 1.22 197 66 70 4 789.40 27.18 198 66 75 9 587.36 1.21 199 66 171 105 335.94 0.94 200 67 171 104 335.97 0.93 201 68 71 3 993.68 0.46 202 69 72 3 876.03 4.41 203 69 73 4 863.19 2.02 204 69 75 6 590.75 2.71 205 70 74 4 607.32 2.33 206 70 75 5 590.56 2.81 207 73 76 3 949.57 1.43 208 73 99 26 584.00 1.50 209 76 81 5 675.65 25.48 210 76 82 6 644.48 17.25 211 76 85 9 657.79 23.91 212 76 99 23 584.09 1.51 213 76 103 27 584.01 1.49 214 77 103 26 583.87 1.41 215 77 194 117 268.56 2.86 216 78 81 3 855.15 5.03 217 78 82 4 825.95 4.87 218 78 103 25 584.10 1.52 219 78 151 73 337.35 0.91 220 78 193 115 268.73 2.82 221 78 194 116 268.55 2.87 222 79 82 3 855.56 4.82 223 79 83 4 839.54 1.93 224 79 100 21 584.17 1.49 225 79 103 24 584.17 1.49 226 79 194 115 268.57 2.87 227 79 195 116 268.49 2.86 228 79 196 117 268.21 2.89 229 79 219 140 199.43 2.60 230 80 83 3 855.39 4.94 231 80 84 4 855.60 4.86 232 80 148 68 337.26 0.88 233 80 152 72 337.31 0.93 234 80 195 115 268.55 2.88 235 80 218 138 199.68 2.66 236 80 219 139 198.77 2.92 237 80 222 142 182.53 3.10 238 81 84 3 858.86 3.24 239 81 85 4 858.89 3.26 240 81 151 70 337.31 0.93 241 81 152 71 337.19 0.96 242 81 155 74 337.22 0.95 243 82 85 3 873.68 4.08 244 82 86 4 863.16 1.12 245 82 96 14 584.21 1.48 246 82 99 17 584.22 1.48 247 82 100 18 584.18 1.49 248 82 103 21 584.20 1.48 249 83 86 3 886.14 2.16 250 83 87 4 871.38 5.23 251 83 219 136 198.80 2.91 252 83 222 139 182.53 3.10 253 84 87 3 890.12 0.17 254 84 88 4 863.13 1.12 255 84 152 68 337.19 0.96 256 84 222 138 182.50 3.12 257 84 226 142 175.09 3.21 258 85 88 3 868.97 4.03 259 85 89 4 847.86 0.88 260 85 90 5 786.52 4.59 261 85 91 6 717.29 8.00 262 85 155 70 337.11 0.91 263 85 156 71 337.03 0.88 264 85 159 74 336.70 0.93 265 86 89 3 849.62 1.79 266 86 91 5 698.85 39.25 267 86 92 6 660.81 20.25 268 86 93 7 597.38 0.65 269 86 96 10 595.91 0.82 270 87 223 136 182.49 3.12 271 87 226 139 175.16 3.25 272 88 156 68 337.03 0.88 273 88 226 138 174.80 3.35 274 88 230 142 146.74 0.83 275 88 236 148 102.17 1.81 276 89 243 154 51.48 1.26 277 89 246 157 51.22 1.32 278 90 156 66 337.05 0.89 279 90 236 146 97.98 0.09 280 90 246 156 51.20 1.31 281 91 95 4 847.88 6.49 282 91 96 5 840.87 2.98 283 91 246 155 51.20 1.31 284 92 95 3 853.11 3.88 285 92 96 4 853.61 3.63 286 92 246 154 51.20 1.31 287 92 247 155 51.06 1.29 288 92 248 156 51.03 1.29 289 92 249 157 50.04 1.46 290 93 96 3 859.23 0.82 291 93 97 4 847.89 0.77 292 93 247 154 51.08 1.28 293 94 97 3 870.77 0.67 294 94 98 4 847.94 0.76 295 95 98 3 878.22 4.21 296 95 99 4 847.95 0.77 297 96 99 3 874.58 6.03 298 96 100 4 847.95 0.77 299 97 100 3 864.42 0.51 300 97 101 4 856.33 1.83 301 98 101 3 882.08 6.32 302 98 102 4 851.14 0.76 303 99 102 3 864.83 4.51 304 99 103 4 847.94 0.84 305 100 103 3 875.26 9.72 306 100 104 4 851.06 2.38 307 101 104 3 864.63 4.41 308 101 105 4 847.98 0.83 309 102 105 3 872.34 0.56 310 102 106 4 849.38 1.72 311 103 106 3 875.73 2.25 312 103 107 4 846.49 0.02 313 103 196 93 268.16 2.88 314 104 107 3 872.34 0.56 315 104 108 4 846.49 0.01 316 104 196 92 268.15 2.88 317 104 199 95 266.69 2.94 318 104 215 111 201.95 3.00 319 105 108 3 858.83 0.65 320 105 109 4 844.74 0.85 321 106 109 3 858.82 0.65 322 106 110 4 840.11 2.11 323 107 110 3 848.01 6.06 324 107 111 4 839.75 2.30 325 107 196 89 268.20 2.89 326 107 197 90 268.14 2.88 327 107 200 93 264.45 2.21 328 108 111 3 871.72 0.93 329 108 112 4 852.99 8.45 330 108 199 91 268.05 2.89 331 108 200 92 266.31 2.81 332 108 203 95 250.12 1.30 333 108 204 96 248.53 1.07 334 109 112 3 872.57 0.70 335 109 113 4 871.72 0.92 336 110 113 3 871.73 0.92 337 110 114 4 871.70 0.93 338 111 114 3 875.65 1.04 339 111 115 4 875.58 1.07 340 111 126 15 341.90 1.09 341 111 200 89 266.62 2.93 342 111 201 90 266.31 2.81 343 111 204 93 248.55 1.07 344 112 115 3 875.87 0.94 345 112 204 92 248.55 1.06 346 113 116 3 889.29 5.78 347 113 117 4 853.29 3.89 348 114 117 3 853.73 3.68 349 114 118 4 845.06 6.35 350 114 126 12 342.08 1.12 351 115 118 3 877.84 8.38 352 115 119 4 833.78 4.07 353 115 126 11 342.10 1.13 354 115 204 89 263.66 2.33 355 116 119 3 855.06 1.77 356 118 121 3 873.86 0.27 357 118 124 6 467.54 62.01 358 118 126 8 342.23 1.14 359 121 124 3 865.97 3.41 360 125 128 3 867.04 1.45 361 126 201 75 267.90 2.81 362 126 204 78 265.47 2.39 363 126 205 79 259.38 0.21 364 127 197 70 268.90 2.75 365 127 201 74 268.01 2.79 366 128 201 73 268.12 2.87 367 128 202 74 267.95 2.81 368 128 205 77 263.66 2.33 369 128 207 79 248.41 1.03 370 129 189 60 310.60 1.56 371 129 190 61 307.94 0.71 372 129 191 62 307.94 0.70 373 129 192 63 307.89 0.69 374 129 197 68 268.96 2.77 375 129 198 69 268.58 2.84 376 129 214 85 202.56 3.17 377 130 189 59 310.64 1.55 378 131 135 4 811.41 6.42 379 131 137 6 340.84 0.19 380 131 189 58 314.25 0.24 381 132 135 3 867.15 1.27 382 132 187 55 331.54 0.60 383 132 188 56 325.26 2.24 384 135 140 5 591.48 101.75 385 135 188 53 326.43 1.93 386 136 139 3 888.09 0.58 387 136 140 4 819.78 12.39 388 137 140 3 829.73 7.42 389 138 141 3 861.31 0.18 390 140 143 3 899.00 4.89 391 140 189 49 325.82 1.95 392 140 213 73 203.19 3.05 393 141 144 3 876.83 1.43 394 141 145 4 861.27 0.03 395 141 212 71 203.16 3.04 396 141 213 72 202.54 3.18 397 141 216 75 202.58 3.15 398 141 217 76 202.16 3.06 399 142 145 3 868.68 5.50 400 142 146 4 856.90 2.16 401 143 146 3 872.98 0.57 402 143 147 4 855.94 2.62 403 143 184 41 334.62 1.07 404 143 185 42 330.35 0.26 405 143 188 45 319.51 4.39 406 143 189 46 310.67 1.53 407 144 147 3 874.00 0.01 408 144 148 4 846.62 2.02 409 144 189 45 309.99 1.75 410 144 191 47 275.57 5.80 411 144 192 48 269.35 2.74 412 144 193 49 269.19 2.68 413 144 213 69 202.53 3.18 414 144 214 70 202.41 3.17 415 144 217 73 201.30 3.15 416 144 218 74 201.27 3.15 417 145 148 3 881.99 2.48 418 145 149 4 864.24 0.92 419 145 217 72 201.33 3.13 420 145 220 75 199.72 2.65 421 145 221 76 198.94 2.45 422 146 149 3 866.80 2.19 423 146 150 4 866.80 2.19 424 147 150 3 893.22 1.63 425 147 151 4 872.47 0.42 426 147 184 37 333.36 1.27 427 147 193 46 268.94 2.75 428 148 151 3 871.23 0.20 429 148 152 4 859.05 3.50 430 148 193 45 268.89 2.74 431 148 218 70 199.72 2.65 432 149 152 3 866.07 2.78 433 149 153 4 859.02 3.49 434 149 221 72 190.72 2.98 435 149 224 75 182.47 3.12 436 149 225 76 181.72 3.25 437 149 228 79 175.23 3.25 438 150 153 3 866.96 2.33 439 150 154 4 859.03 3.48 440 150 177 27 335.72 0.90 441 150 180 30 335.35 1.04 442 150 181 31 335.03 1.03 443 151 154 3 875.25 0.98 444 151 155 4 842.50 4.79 445 151 174 23 336.05 0.90 446 151 177 26 335.57 0.97 447 151 178 27 335.56 0.97 448 151 193 42 268.89 2.74 449 152 155 3 870.12 7.72 450 152 156 4 842.49 4.79 451 152 222 70 182.53 3.10 452 152 225 73 175.27 3.24 453 153 156 3 873.46 6.05 454 153 157 4 852.12 3.33 455 153 225 72 175.28 3.23 456 153 229 76 163.94 2.40 457 154 157 3 880.31 2.63 458 154 158 4 845.75 6.52 459 154 177 23 335.65 0.89 460 155 158 3 861.07 1.14 461 155 159 4 845.76 6.52 462 155 174 19 335.85 0.86 463 156 159 3 862.88 0.87 464 156 160 4 842.61 8.09 465 156 225 69 175.73 3.04 466 156 226 70 175.25 3.23 467 156 229 73 154.47 1.06 468 157 160 3 857.27 0.74 469 157 163 6 802.03 0.33 470 157 229 72 158.30 0.84 471 158 166 8 337.31 0.42 472 158 170 12 335.96 0.92 473 158 174 16 335.83 0.86 474 160 163 3 888.57 1.47 475 160 229 69 168.28 2.59 476 162 234 72 133.52 3.04 477 162 235 73 125.29 1.07 478 162 236 74 122.30 0.39 479 163 166 3 916.36 0.33 480 163 229 66 175.13 3.33 481 169 172 3 869.88 1.54 482 169 173 4 846.47 7.81 483 170 173 3 867.87 0.54 484 170 174 4 861.52 0.35 485 171 174 3 872.75 1.91 486 171 175 4 857.41 2.38 487 172 175 3 861.51 0.35 488 172 176 4 857.41 2.38 489 173 176 3 874.18 10.75 490 173 177 4 858.35 1.92 491 174 177 3 859.61 2.54 492 174 178 4 858.35 1.86 493 175 178 3 859.09 2.23 494 175 179 4 851.82 1.41 495 176 179 3 853.63 1.44 496 176 180 4 850.31 3.10 497 177 180 3 860.84 2.15 498 177 181 4 860.84 2.15 499 178 181 3 863.89 2.62 500 178 182 4 860.87 2.14 501 179 182 3 863.26 0.94 502 179 183 4 860.30 2.41 503 180 183 3 861.87 1.61 504 180 184 4 860.28 2.40 505 181 184 3 877.07 1.84 506 181 185 4 857.71 1.11 507 181 191 10 269.34 2.74 508 181 193 12 268.95 2.77 509 182 185 3 853.30 9.53 510 182 186 4 797.00 5.59 511 182 191 9 269.31 2.73 512 182 193 11 269.24 2.74 513 184 188 4 873.06 1.85 514 185 188 3 873.06 1.85 515 185 189 4 799.21 3.90 516 185 190 5 684.07 33.26 517 185 191 6 269.36 2.73 518 186 190 4 514.56 69.27 519 186 191 5 360.20 7.49 520 189 214 25 202.94 3.16 521 192 197 5 762.47 9.29 522 192 198 6 399.50 10.52 523 193 218 25 199.71 2.65 524 194 197 3 875.79 2.41 525 194 198 4 843.59 3.23 526 194 218 24 199.70 2.65 527 195 198 3 869.36 3.39 528 195 199 4 828.05 4.58 529 195 214 19 201.72 3.11 530 195 215 20 201.66 3.10 531 195 218 23 199.71 2.65 532 195 219 24 199.67 2.65 533 196 199 3 862.72 0.04 534 196 200 4 845.50 5.45 535 197 200 3 882.24 3.06 536 197 201 4 869.34 7.81 537 198 201 3 884.69 1.83 538 198 202 4 846.34 5.03 539 198 214 16 202.02 3.02 540 199 202 3 886.69 0.83 541 199 203 4 844.88 4.31 542 199 208 9 375.18 2.17 543 199 211 12 202.52 3.18 544 199 215 16 202.00 3.02 545 200 203 3 889.85 2.41 546 200 204 4 861.75 3.70 547 201 204 3 878.62 0.84 548 201 205 4 850.17 2.09 549 202 205 3 853.89 0.24 550 202 206 4 824.39 3.60 551 202 207 5 717.87 22.52 552 202 208 6 606.33 3.02 553 202 211 9 202.59 3.15 554 203 206 3 831.63 11.36 555 203 208 5 803.85 6.68 556 208 211 3 796.98 4.46 557 210 213 3 875.46 0.07 558 211 214 3 870.32 0.70 559 211 215 4 870.31 0.70 560 212 215 3 872.08 0.18 561 212 216 4 853.51 1.35 562 213 216 3 869.07 0.07 563 213 217 4 840.20 4.91 564 213 218 5 840.60 5.11 565 214 218 4 842.58 3.62 566 215 218 3 869.82 0.34 567 215 219 4 836.59 6.48 568 216 220 4 826.59 10.75 569 217 220 3 852.13 8.38 570 217 221 4 840.65 7.92 571 218 221 3 856.51 6.49 572 218 222 4 836.46 5.83 573 219 222 3 868.49 7.79 574 219 223 4 845.80 1.16 575 220 223 3 868.81 7.63 576 220 224 4 845.81 1.16 577 221 224 3 863.79 10.13 578 221 225 4 845.76 1.13 579 222 225 3 851.64 4.06 580 222 226 4 840.86 1.33 581 223 226 3 869.68 8.00 582 223 227 4 831.40 3.12 583 224 227 3 866.62 1.17 584 224 228 4 820.15 2.50 585 225 228 3 866.62 1.17 586 225 229 4 831.33 3.08 587 226 229 3 869.34 2.53 588 226 230 4 825.56 5.96 589 226 231 5 825.97 6.17 590 227 231 4 810.15 1.74 591 227 232 5 783.58 0.74 592 228 232 4 732.85 9.37 593 228 233 5 732.84 9.37 594 229 233 4 718.31 16.64 595 229 234 5 536.38 44.11 596 229 235 6 536.47 44.16 597 230 234 4 542.61 47.22 598 230 235 5 410.44 9.48 599 230 236 6 410.44 9.48 600 231 235 4 410.43 9.48 601 231 243 12 365.56 1.12 602 236 243 7 784.72 4.05 603 238 242 4 878.36 3.26 604 238 243 5 846.43 5.62 605 239 242 3 894.46 2.06 606 239 243 4 862.90 10.99 607 240 243 3 864.46 10.21 608 240 244 4 862.90 10.99 609 241 244 3 866.70 4.76 610 241 245 4 862.01 2.99 611 242 245 3 871.02 6.93 612 243 246 3 877.66 2.02 613 244 247 3 869.49 6.11 614 245 248 3 891.61 3.35 615 245 249 4 859.34 1.03 616 246 249 3 871.74 5.17