BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 116.78 0.18 2 3 228 225 115.42 0.35 3 3 229 226 111.38 0.58 4 4 204 200 170.68 0.12 5 4 205 201 170.62 0.14 6 4 206 202 170.09 0.01 7 4 207 203 169.28 0.05 8 4 229 225 117.02 0.08 9 5 36 31 511.13 18.46 10 5 37 32 511.13 18.46 11 5 38 33 511.12 18.47 12 5 207 202 170.09 0.02 13 6 36 30 511.25 18.46 14 6 37 31 511.18 18.45 15 6 38 32 511.18 18.44 16 6 223 217 117.31 0.01 17 6 229 223 117.25 0.03 18 6 249 243 22.29 0.01 19 7 37 30 511.24 18.46 20 7 38 31 511.19 18.44 21 7 40 33 511.18 18.44 22 7 90 83 272.39 41.75 23 7 163 156 259.44 38.16 24 7 207 200 182.66 0.15 25 7 209 202 170.32 0.14 26 7 229 222 117.41 0.02 27 7 230 223 117.26 0.04 28 7 231 224 117.25 0.04 29 8 37 29 511.31 18.44 30 8 38 30 511.24 18.46 31 8 39 31 511.18 18.45 32 8 40 32 511.18 18.45 33 8 231 223 117.28 0.03 34 8 242 234 53.72 2.00 35 8 246 238 41.17 0.21 36 9 40 31 511.18 18.45 37 9 124 115 270.61 42.24 38 9 231 222 117.40 0.01 39 9 232 223 117.25 0.03 40 9 233 224 117.25 0.03 41 9 242 233 54.91 1.43 42 10 40 30 511.32 18.43 43 10 41 31 511.27 18.41 44 10 42 32 511.31 18.39 45 10 233 223 117.26 0.03 46 10 237 227 84.12 0.03 47 10 242 232 54.91 1.43 48 11 42 31 511.51 18.29 49 11 65 54 469.09 1.79 50 11 95 84 271.37 42.12 51 11 232 221 118.03 0.00 52 11 233 222 117.34 0.03 53 11 234 223 117.25 0.03 54 11 237 226 91.59 0.57 55 12 15 3 888.14 2.93 56 12 21 9 512.81 18.22 57 12 24 12 512.77 18.21 58 12 25 13 512.77 18.21 59 12 28 16 512.76 18.21 60 12 41 29 511.59 18.30 61 12 42 30 511.57 18.29 62 12 43 31 511.55 18.27 63 12 51 39 472.58 1.81 64 12 65 53 469.74 2.12 65 12 237 225 111.34 0.73 66 12 238 226 88.03 1.21 67 13 44 31 512.04 18.05 68 13 65 52 469.68 2.16 69 13 97 84 271.80 41.93 70 13 234 221 118.03 0.00 71 14 44 30 512.04 18.05 72 15 44 29 512.18 18.00 73 15 238 223 117.20 0.04 74 16 20 4 844.46 4.11 75 16 21 5 764.79 34.19 76 16 47 31 512.22 17.84 77 17 20 3 858.83 3.08 78 17 21 4 845.37 3.65 79 18 21 3 875.12 3.02 80 18 22 4 845.71 3.48 81 18 50 32 510.81 17.02 82 19 22 3 870.83 1.45 83 19 23 4 858.86 3.06 84 20 23 3 879.51 3.13 85 20 24 4 860.05 3.66 86 21 24 3 865.19 1.08 87 21 25 4 851.97 0.38 88 21 47 26 512.13 17.62 89 21 238 217 111.77 0.11 90 22 25 3 864.67 1.34 91 22 26 4 855.95 5.71 92 22 50 28 477.61 0.00 93 22 54 32 473.31 2.29 94 23 26 3 856.46 5.44 95 23 27 4 855.93 5.70 96 24 27 3 878.09 1.96 97 24 28 4 848.65 3.46 98 24 237 213 112.18 0.07 99 24 238 214 104.63 0.68 100 24 239 215 87.32 1.56 101 25 28 3 864.51 8.75 102 25 29 4 848.69 3.44 103 25 51 26 473.29 2.21 104 25 54 29 472.45 2.71 105 25 55 30 472.36 2.76 106 26 29 3 851.30 2.14 107 26 30 4 848.69 3.44 108 26 54 28 472.53 2.74 109 27 30 3 848.68 3.44 110 27 31 4 847.52 4.01 111 27 240 213 79.67 0.41 112 27 243 216 57.75 2.41 113 28 31 3 848.11 4.30 114 28 55 27 472.46 2.81 115 28 57 29 472.29 2.91 116 28 60 32 472.23 2.93 117 28 242 214 57.76 2.41 118 29 54 25 472.56 2.79 119 29 55 26 472.48 2.82 120 29 56 27 472.45 2.83 121 29 57 28 472.29 2.91 122 31 57 26 472.38 2.95 123 31 243 212 52.88 1.63 124 33 37 4 889.55 3.04 125 33 39 6 544.47 3.71 126 33 57 24 507.90 20.73 127 33 59 26 493.27 13.48 128 33 60 27 493.30 13.47 129 33 246 213 44.73 0.78 130 34 59 25 521.75 27.70 131 35 59 24 523.64 28.68 132 35 246 211 44.73 0.78 133 35 249 214 36.35 0.46 134 35 250 215 26.11 0.10 135 37 59 22 523.41 28.86 136 37 246 209 41.17 0.21 137 37 249 212 26.07 0.28 138 38 59 21 523.42 28.87 139 38 60 22 523.40 28.86 140 38 61 23 523.38 28.86 141 38 90 52 272.54 41.74 142 39 59 20 523.44 28.87 143 39 60 21 523.38 28.86 144 39 61 22 523.40 28.87 145 39 246 207 41.17 0.21 146 40 61 21 523.41 28.87 147 40 63 23 523.33 28.88 148 40 90 50 272.54 41.74 149 40 124 84 270.30 42.29 150 41 51 10 523.97 28.63 151 41 55 14 523.66 28.79 152 41 60 19 523.49 28.87 153 41 61 20 523.44 28.87 154 41 62 21 523.38 28.86 155 41 63 22 523.35 28.88 156 42 63 21 523.35 28.88 157 42 64 22 523.34 28.88 158 42 65 23 523.23 28.87 159 42 92 50 272.52 41.73 160 43 47 4 829.88 3.98 161 43 48 5 700.50 59.51 162 43 51 8 565.57 7.92 163 43 63 20 523.42 28.88 164 43 65 22 523.33 28.88 165 44 47 3 856.87 3.42 166 44 48 4 848.91 0.57 167 44 65 21 523.34 28.89 168 45 48 3 866.75 0.99 169 45 64 19 523.41 28.89 170 45 66 21 523.40 28.88 171 45 78 33 292.19 40.38 172 45 83 38 291.27 40.02 173 47 50 3 867.41 0.24 174 47 51 4 855.36 5.78 175 48 51 3 855.53 5.70 176 48 52 4 855.93 5.50 177 48 62 14 523.79 28.75 178 48 63 15 523.78 28.75 179 48 64 16 523.74 28.75 180 48 83 35 288.58 39.91 181 48 86 38 283.55 40.30 182 48 88 40 277.89 43.04 183 49 52 3 865.00 7.50 184 49 53 4 856.13 5.39 185 49 86 37 283.25 40.45 186 50 53 3 877.85 4.50 187 50 54 4 846.55 0.59 188 51 54 3 863.68 2.04 189 51 55 4 841.13 2.10 190 51 62 11 523.84 28.75 191 52 55 3 881.38 4.67 192 52 62 10 523.85 28.75 193 52 86 34 283.16 40.47 194 55 60 5 527.22 27.12 195 55 62 7 523.89 28.75 196 57 60 3 869.26 4.55 197 60 246 186 41.90 0.13 198 61 90 29 272.54 41.74 199 62 88 26 272.74 41.70 200 62 90 28 272.55 41.74 201 63 88 25 273.82 41.60 202 63 90 27 272.55 41.74 203 63 91 28 272.67 41.69 204 64 83 19 283.07 40.43 205 64 88 24 274.50 41.92 206 64 90 26 273.92 41.84 207 64 91 27 272.74 41.69 208 64 92 28 272.79 41.66 209 65 92 27 273.93 42.05 210 66 83 17 291.90 40.27 211 66 91 25 274.53 41.91 212 66 92 26 274.49 41.89 213 66 93 27 274.38 41.88 214 66 98 32 274.62 41.93 215 66 112 46 275.46 41.60 216 67 78 11 292.49 40.45 217 67 79 12 292.26 40.36 218 67 80 13 292.25 40.36 219 67 83 16 292.05 40.26 220 67 91 24 274.75 41.94 221 67 93 26 274.72 41.93 222 67 112 45 276.25 41.23 223 68 74 6 398.92 36.24 224 68 77 9 292.63 40.43 225 68 78 10 292.56 40.41 226 68 79 11 292.45 40.43 227 68 80 12 292.47 40.44 228 68 112 44 275.74 41.74 229 69 80 11 292.49 40.45 230 69 81 12 292.56 40.41 231 69 115 46 281.53 40.23 232 70 74 4 922.12 2.89 233 70 79 9 292.72 40.41 234 71 74 3 968.89 0.05 235 71 79 8 292.72 40.41 236 71 81 10 292.72 40.41 237 71 82 11 292.69 40.40 238 74 77 3 870.34 1.31 239 78 82 4 846.27 0.29 240 78 83 5 831.82 2.12 241 79 82 3 860.14 1.41 242 79 83 4 846.27 0.29 243 80 83 3 859.52 1.10 244 80 84 4 839.66 2.90 245 80 91 11 308.43 34.41 246 80 115 35 353.79 11.86 247 80 116 36 349.87 13.71 248 80 119 39 438.29 22.80 249 80 121 41 438.28 22.80 250 81 84 3 851.22 2.19 251 81 85 4 842.19 1.64 252 82 85 3 853.55 4.71 253 82 86 4 835.30 4.04 254 83 86 3 847.25 4.17 255 83 87 4 835.24 4.10 256 83 88 5 671.04 0.80 257 83 91 8 520.62 18.99 258 83 121 38 583.41 0.73 259 84 87 3 841.70 1.43 260 84 119 35 583.44 0.73 261 84 121 37 583.43 0.73 262 88 121 33 660.38 1.65 263 89 120 31 660.39 1.64 264 89 121 32 660.34 1.66 265 89 122 33 660.29 1.68 266 90 121 31 660.38 1.64 267 90 122 32 660.35 1.65 268 90 124 34 660.25 1.68 269 91 116 25 660.47 1.63 270 91 121 30 660.45 1.62 271 91 122 31 660.44 1.62 272 91 123 32 660.34 1.66 273 91 124 33 660.31 1.67 274 92 95 3 914.62 0.94 275 92 124 32 660.34 1.66 276 92 126 34 660.25 1.69 277 93 112 19 660.61 1.62 278 93 113 20 660.54 1.63 279 93 116 23 660.59 1.64 280 93 123 30 660.48 1.63 281 93 124 31 660.43 1.62 282 93 125 32 660.34 1.66 283 93 126 33 660.27 1.68 284 94 99 5 827.40 1.00 285 94 104 10 660.61 1.61 286 94 109 15 660.60 1.62 287 94 126 32 660.30 1.67 288 95 98 3 867.06 4.83 289 95 99 4 850.14 1.50 290 95 126 31 660.41 1.63 291 95 167 72 660.20 1.65 292 95 232 137 121.84 0.31 293 96 99 3 855.26 1.06 294 96 100 4 850.13 1.50 295 96 167 71 660.35 1.64 296 96 169 73 653.45 0.40 297 97 100 3 855.26 1.06 298 97 101 4 850.12 1.51 299 97 102 5 827.50 0.98 300 98 101 3 862.69 4.71 301 98 102 4 827.41 0.97 302 98 103 5 827.41 0.97 303 98 104 6 661.38 1.43 304 99 104 5 660.93 1.65 305 99 106 7 662.22 2.04 306 99 109 10 660.94 1.65 307 99 126 27 660.60 1.61 308 99 127 28 660.56 1.60 309 99 146 47 660.62 1.62 310 103 108 5 860.63 1.68 311 104 108 4 851.53 6.23 312 104 109 5 830.54 0.34 313 104 112 8 823.09 0.28 314 105 108 3 875.88 0.35 315 105 109 4 860.62 1.68 316 106 109 3 867.27 3.96 317 106 110 4 860.64 1.69 318 106 145 39 660.97 1.72 319 106 146 40 660.74 1.68 320 106 149 43 661.02 1.72 321 107 110 3 860.65 1.69 322 107 111 4 860.65 1.69 323 108 111 3 861.75 1.15 324 108 112 4 861.75 1.15 325 109 112 3 861.75 1.15 326 109 113 4 861.75 1.15 327 110 113 3 870.62 2.66 328 110 114 4 861.68 1.10 329 110 149 39 661.20 1.65 330 110 150 40 661.02 1.71 331 110 153 43 661.81 1.82 332 111 114 3 867.70 4.12 333 111 115 4 854.24 2.62 334 111 153 42 662.07 1.82 335 112 115 3 866.06 4.94 336 112 116 4 851.69 4.21 337 113 116 3 865.52 0.14 338 113 117 4 852.65 3.00 339 113 123 10 661.65 1.80 340 113 125 12 660.96 1.73 341 113 150 37 661.64 1.80 342 113 154 41 662.69 2.00 343 113 162 49 661.95 1.66 344 114 117 3 875.75 4.31 345 114 118 4 852.68 3.00 346 114 153 39 662.25 1.85 347 114 154 40 662.61 2.01 348 115 118 3 875.75 4.31 349 115 119 4 862.89 2.12 350 116 119 3 866.62 8.88 351 116 120 4 850.71 0.93 352 116 121 5 681.00 1.12 353 116 123 7 662.68 2.02 354 117 120 3 842.44 1.18 355 117 123 6 669.72 1.29 356 117 154 37 706.15 2.43 357 117 158 41 688.34 6.48 358 117 161 44 677.82 2.77 359 117 162 45 670.56 0.87 360 120 161 41 796.07 3.75 361 121 161 40 796.09 3.75 362 122 160 38 796.16 3.75 363 122 161 39 796.11 3.76 364 122 207 85 252.19 25.03 365 123 161 38 796.15 3.75 366 123 162 39 796.10 3.75 367 123 163 40 796.08 3.75 368 124 163 39 796.08 3.75 369 124 165 41 796.05 3.75 370 124 209 85 251.43 25.38 371 125 150 25 796.36 3.71 372 125 163 38 796.15 3.75 373 125 164 39 796.12 3.75 374 125 165 40 796.09 3.75 375 126 165 39 796.12 3.75 376 126 167 41 796.06 3.75 377 126 232 106 121.42 0.46 378 127 143 16 796.49 3.73 379 127 146 19 796.47 3.73 380 127 147 20 796.45 3.73 381 127 150 23 796.48 3.74 382 127 164 37 796.19 3.75 383 127 165 38 796.15 3.75 384 127 166 39 796.12 3.75 385 127 167 40 796.09 3.75 386 127 190 63 259.41 23.56 387 128 146 18 796.55 3.76 388 128 166 38 796.13 3.75 389 128 167 39 796.10 3.75 390 129 133 4 824.15 3.57 391 129 134 5 810.02 1.02 392 129 139 10 796.56 3.76 393 129 142 13 796.58 3.75 394 129 143 14 796.50 3.73 395 129 166 37 796.19 3.75 396 129 169 40 796.03 3.77 397 129 170 41 795.93 3.79 398 130 133 3 865.70 2.89 399 130 134 4 838.43 2.87 400 130 169 39 796.05 3.77 401 130 170 40 795.95 3.79 402 131 134 3 852.18 4.00 403 131 135 4 838.43 2.87 404 131 170 39 795.93 3.81 405 131 174 43 728.08 0.91 406 131 176 45 685.06 2.63 407 132 135 3 883.43 10.51 408 132 136 4 852.19 4.00 409 133 136 3 870.25 3.92 410 133 137 4 861.31 0.56 411 133 138 5 796.88 3.81 412 133 142 9 796.78 3.79 413 134 137 3 863.65 5.54 414 134 139 5 796.81 3.77 415 134 166 32 796.25 3.73 416 134 170 36 795.96 3.83 417 134 182 48 270.06 19.00 418 138 141 3 867.13 6.17 419 138 142 4 842.36 1.18 420 139 142 3 862.56 2.03 421 139 143 4 842.36 1.18 422 140 143 3 858.41 0.74 423 140 144 4 851.37 4.25 424 140 189 49 259.84 23.55 425 141 145 4 860.97 0.56 426 142 145 3 860.99 0.55 427 142 146 4 860.98 0.56 428 143 146 3 860.98 0.56 429 143 147 4 861.03 0.58 430 143 166 23 796.20 3.73 431 143 189 46 259.80 23.54 432 143 190 47 259.80 23.55 433 144 147 3 868.99 4.58 434 144 148 4 858.13 2.04 435 144 189 45 259.80 23.54 436 144 193 49 259.36 23.54 437 145 148 3 860.10 1.07 438 145 149 4 858.14 2.04 439 146 149 3 872.66 2.69 440 146 150 4 860.09 1.06 441 147 150 3 864.07 0.93 442 147 151 4 848.79 2.59 443 147 159 12 796.87 3.77 444 147 164 17 796.23 3.73 445 147 193 46 259.32 23.52 446 148 151 3 854.62 0.32 447 148 152 4 848.80 2.60 448 148 193 45 259.29 23.50 449 149 152 3 868.02 5.83 450 150 153 3 875.09 2.29 451 150 154 4 848.77 2.60 452 151 154 3 859.48 1.55 453 151 159 8 796.97 3.74 454 151 193 42 259.24 23.48 455 151 198 47 254.25 24.57 456 154 158 4 848.42 3.01 457 154 159 5 847.47 4.29 458 154 162 8 796.82 3.67 459 155 158 3 874.55 0.79 460 155 159 4 863.37 4.80 461 156 159 3 894.62 0.13 462 156 198 42 254.19 24.55 463 156 201 45 254.10 24.59 464 156 202 46 253.71 24.50 465 157 160 3 894.77 0.21 466 158 161 3 888.61 3.14 467 158 162 4 863.00 5.00 468 159 162 3 854.97 1.00 469 159 198 39 254.19 24.61 470 159 202 43 252.84 24.91 471 159 206 47 252.42 25.03 472 162 206 44 252.33 24.99 473 163 207 44 252.09 25.08 474 163 209 46 251.77 25.23 475 164 190 26 259.04 23.57 476 164 206 42 252.50 25.07 477 164 207 43 252.24 25.03 478 164 208 44 251.77 25.23 479 164 209 45 251.44 25.39 480 165 209 44 251.44 25.39 481 165 232 67 121.42 0.46 482 166 186 20 259.51 23.59 483 166 187 21 259.39 23.56 484 166 190 24 259.32 23.52 485 166 208 42 252.04 25.14 486 166 209 43 251.47 25.38 487 166 210 44 251.43 25.38 488 166 211 45 251.33 25.40 489 167 211 44 251.12 25.34 490 168 171 3 876.79 3.70 491 168 172 4 851.60 2.60 492 168 210 42 251.46 25.38 493 168 211 43 251.44 25.38 494 168 212 44 251.31 25.41 495 169 172 3 856.24 2.91 496 169 174 5 851.60 2.60 497 170 174 4 846.69 0.14 498 170 176 6 488.56 3.06 499 170 178 8 272.24 20.02 500 170 182 12 261.31 23.35 501 171 174 3 862.40 0.21 502 171 176 5 316.44 18.74 503 171 178 7 272.24 20.02 504 172 211 39 251.38 25.41 505 172 212 40 251.19 25.33 506 177 182 5 850.27 4.15 507 178 182 4 838.50 1.74 508 178 210 32 252.20 25.20 509 179 182 3 851.93 0.58 510 179 183 4 838.62 1.80 511 180 183 3 853.99 2.65 512 180 184 4 842.18 3.59 513 180 221 41 186.47 31.99 514 180 222 42 172.70 25.12 515 180 225 45 129.68 3.63 516 181 184 3 852.63 1.64 517 181 185 4 841.28 4.04 518 182 185 3 862.44 4.57 519 182 186 4 849.52 0.07 520 183 186 3 865.35 3.12 521 183 187 4 843.71 2.83 522 183 210 27 252.26 25.17 523 183 222 39 172.69 25.12 524 184 187 3 850.38 0.50 525 184 188 4 843.71 2.83 526 184 225 41 126.55 2.26 527 184 226 42 126.18 2.08 528 184 227 43 125.86 1.92 529 185 188 3 856.28 1.16 530 185 189 4 853.07 0.79 531 186 189 3 859.43 0.42 532 186 190 4 854.53 0.05 533 187 190 3 854.54 0.05 534 187 191 4 854.53 0.05 535 187 208 21 252.64 25.16 536 187 209 22 252.64 25.16 537 187 210 23 252.59 25.14 538 187 227 40 125.76 1.91 539 187 228 41 125.75 1.91 540 188 191 3 859.86 2.71 541 188 192 4 841.63 4.97 542 188 227 39 125.76 1.92 543 189 192 3 858.43 3.43 544 189 193 4 849.56 1.02 545 190 193 3 866.30 1.17 546 190 194 4 850.88 1.68 547 190 208 18 252.54 25.09 548 191 194 3 865.69 1.47 549 191 195 4 838.96 6.32 550 191 203 12 253.60 24.93 551 191 206 15 253.34 25.02 552 191 208 17 252.67 25.12 553 191 227 36 125.70 1.94 554 191 229 38 125.69 1.94 555 192 195 3 855.41 0.59 556 192 196 4 838.96 6.32 557 193 196 3 861.41 4.92 558 193 197 4 827.13 4.55 559 193 198 5 827.13 4.55 560 194 197 3 852.73 1.85 561 194 198 4 815.08 1.47 562 194 199 5 813.66 2.17 563 194 202 8 309.37 21.73 564 194 203 9 282.38 33.45 565 194 206 12 253.60 24.89 566 195 199 4 686.33 54.10 567 195 200 5 311.17 22.59 568 195 203 8 265.56 24.02 569 198 202 4 850.15 0.57 570 199 202 3 869.49 5.41 571 199 203 4 856.46 2.60 572 200 203 3 856.46 2.60 573 200 204 4 847.51 1.88 574 201 204 3 861.54 1.30 575 201 205 4 849.83 2.25 576 202 205 3 860.28 0.68 577 202 206 4 839.00 6.13 578 203 206 3 860.28 0.68 579 206 229 23 123.33 0.94 580 207 229 22 123.20 0.97 581 208 227 19 125.34 1.75 582 208 228 20 123.52 0.85 583 208 229 21 123.42 0.85 584 209 229 20 123.44 0.84 585 209 230 21 122.37 0.56 586 209 231 22 122.35 0.56 587 209 232 23 122.32 0.58 588 210 214 4 894.12 4.15 589 210 222 12 126.05 2.11 590 210 228 18 125.09 1.63 591 210 230 20 123.11 0.68 592 210 231 21 122.42 0.55 593 210 232 22 122.38 0.57 594 211 232 21 122.36 0.56 595 213 218 5 858.58 1.73 596 214 218 4 846.74 4.20 597 214 219 5 808.33 2.91 598 214 222 8 334.82 29.16 599 214 231 17 123.09 0.56 600 214 236 22 122.42 0.53 601 214 245 31 52.78 1.54 602 215 218 3 878.35 6.41 603 215 219 4 846.74 4.20 604 215 236 21 122.62 0.49 605 215 241 26 54.76 1.51 606 215 245 30 53.84 1.74 607 216 220 4 884.96 3.10 608 216 241 25 54.54 1.60 609 216 244 28 53.52 1.90 610 216 245 29 53.51 1.91 611 217 220 3 919.71 1.01 612 217 221 4 884.96 3.10 613 217 241 24 54.76 1.51 614 218 221 3 878.00 6.58 615 218 222 4 851.59 1.73 616 219 222 3 862.50 1.17 617 219 223 4 850.22 1.06 618 219 231 12 125.72 1.81 619 219 245 26 52.09 1.71 620 219 248 29 35.70 0.84 621 220 223 3 856.73 2.44 622 220 224 4 852.51 0.33 623 220 248 28 35.24 1.07 624 221 224 3 852.78 0.48 625 222 225 3 884.33 4.33 626 222 228 6 174.58 21.99 627 222 231 9 125.86 1.88 628 223 228 5 174.58 22.00 629 223 229 6 174.58 21.99 630 223 230 7 174.55 21.98 631 223 231 8 174.55 21.98 632 223 248 25 35.20 1.05 633 223 249 26 26.92 0.06 634 225 228 3 872.96 5.44 635 228 231 3 1029.70 4.00 636 230 249 19 23.42 0.57 637 231 245 14 45.58 1.64 638 231 249 18 25.71 0.10 639 233 236 3 895.24 2.43 640 233 237 4 702.27 73.81 641 233 242 9 137.59 39.91 642 233 245 12 136.62 40.38 643 234 237 3 874.33 4.61 644 235 238 3 896.25 1.92 645 236 239 3 861.91 6.00 646 236 241 5 226.47 4.04 647 236 242 6 183.09 25.73 648 236 245 9 174.60 21.48 649 237 242 5 226.47 4.04 650 239 242 3 874.84 0.53 651 240 243 3 894.18 1.14 652 241 244 3 863.49 0.28 653 241 245 4 851.88 2.88 654 242 245 3 847.20 0.54 655 242 246 4 849.02 0.38 656 243 246 3 857.47 0.94 657 243 247 4 849.02 0.38 658 244 247 3 851.94 1.82 659 244 248 4 849.03 0.38 660 245 248 3 864.51 3.97 661 245 249 4 864.51 3.96 662 246 249 3 880.91 1.85 663 247 250 3 892.28 3.18