BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 136 134 28.48 0.34 2 2 137 135 27.91 0.54 3 2 138 136 28.84 0.35 4 2 141 139 28.75 0.30 5 3 134 131 28.85 0.35 6 3 135 132 28.85 0.35 7 3 136 133 28.84 0.35 8 3 137 134 28.84 0.35 9 3 138 135 28.85 0.35 10 3 168 165 28.91 0.38 11 5 9 4 813.34 4.27 12 5 10 5 745.58 21.40 13 5 13 8 600.98 93.70 14 5 135 130 37.08 1.69 15 5 136 131 34.07 0.18 16 6 9 3 866.46 0.58 17 6 10 4 759.51 22.24 18 6 185 179 45.00 0.17 19 7 10 3 853.60 7.01 20 7 11 4 822.10 0.10 21 8 11 3 828.26 2.33 22 8 12 4 818.76 1.56 23 8 57 49 46.27 0.21 24 8 88 80 46.87 0.04 25 8 89 81 46.89 0.03 26 8 112 104 47.02 0.03 27 8 184 176 46.37 0.24 28 8 185 177 46.38 0.24 29 8 187 179 46.34 0.24 30 9 12 3 829.68 3.03 31 9 13 4 820.08 0.89 32 9 112 103 46.51 0.20 33 9 135 126 46.99 0.03 34 9 136 127 47.33 0.14 35 9 184 175 46.38 0.24 36 9 185 176 46.40 0.24 37 10 13 3 829.68 3.02 38 10 14 4 818.74 1.54 39 11 14 3 829.35 5.66 40 11 15 4 827.73 8.71 41 11 87 76 52.31 0.06 42 11 88 77 51.56 0.12 43 12 15 3 825.50 7.59 44 12 16 4 812.97 1.33 45 12 85 73 51.57 0.12 46 12 88 76 51.56 0.13 47 12 112 100 51.98 0.08 48 13 16 3 818.80 0.88 49 13 17 4 813.66 1.69 50 13 114 101 61.86 0.15 51 13 136 123 59.02 0.50 52 14 17 3 841.90 0.56 53 14 18 4 812.80 2.12 54 15 18 3 836.97 1.92 55 15 19 4 810.69 2.19 56 15 85 70 64.77 1.18 57 15 87 72 62.40 0.01 58 16 19 3 812.86 1.09 59 16 112 96 65.78 0.72 60 16 114 98 70.12 0.12 61 16 115 99 73.24 1.57 62 16 118 102 70.22 0.07 63 17 114 97 70.17 0.10 64 17 115 98 73.17 1.60 65 18 115 97 74.63 2.09 66 19 84 65 74.52 2.14 67 19 85 66 74.50 2.14 68 19 86 67 74.50 2.14 69 19 93 74 76.03 1.54 70 19 111 92 74.52 2.13 71 19 115 96 75.70 1.57 72 20 93 73 76.18 1.57 73 21 117 96 76.19 1.58 74 21 121 100 76.20 1.57 75 22 93 71 76.19 1.56 76 22 97 75 76.21 1.57 77 22 121 99 76.21 1.57 78 22 124 102 90.02 0.41 79 22 125 103 76.21 1.57 80 24 124 100 106.89 3.59 81 26 29 3 944.61 4.28 82 26 30 4 894.25 5.29 83 26 123 97 135.12 0.85 84 26 124 98 134.69 0.95 85 26 126 100 134.65 0.97 86 26 127 101 135.17 0.87 87 26 128 102 137.58 0.24 88 27 126 99 137.55 0.21 89 28 100 72 137.59 0.21 90 28 103 75 138.13 0.06 91 28 107 79 143.60 0.83 92 28 126 98 137.60 0.22 93 28 128 100 146.58 0.66 94 30 123 93 158.44 0.36 95 30 128 98 147.86 1.29 96 30 152 122 159.09 0.21 97 30 153 123 159.12 0.20 98 30 154 124 159.08 0.21 99 31 104 73 158.44 0.37 100 31 106 75 153.83 0.37 101 31 128 97 158.80 0.24 102 32 35 3 837.53 0.85 103 32 36 4 823.22 0.86 104 32 106 74 158.97 0.21 105 33 36 3 832.36 3.45 106 33 37 4 823.23 0.87 107 33 49 16 159.02 0.23 108 33 78 45 159.03 0.23 109 33 79 46 158.97 0.21 110 33 106 73 159.02 0.23 111 33 108 75 159.06 0.22 112 33 129 96 159.12 0.20 113 33 176 143 167.07 0.61 114 34 37 3 823.24 0.87 115 34 38 4 823.23 0.86 116 34 155 121 167.45 0.79 117 34 176 142 167.06 0.61 118 34 206 172 167.42 0.79 119 35 38 3 823.50 0.74 120 35 39 4 820.71 1.91 121 36 39 3 830.67 2.85 122 36 40 4 808.10 1.28 123 36 77 41 167.68 0.81 124 36 78 42 167.65 0.82 125 36 105 69 167.65 0.82 126 36 106 70 167.63 0.82 127 37 40 3 814.77 5.13 128 37 41 4 807.99 1.76 129 37 47 10 167.75 0.79 130 37 49 12 167.75 0.79 131 37 78 41 167.74 0.79 132 37 176 139 167.98 0.80 133 37 206 169 167.97 0.79 134 37 208 171 167.83 0.77 135 37 228 191 167.83 0.77 136 37 229 192 167.80 0.78 137 38 41 3 823.56 0.76 138 38 42 4 814.43 5.33 139 38 206 168 168.06 0.85 140 39 42 3 829.26 4.48 141 39 43 4 813.50 5.79 142 40 43 3 829.90 4.16 143 40 44 4 801.13 0.40 144 40 45 5 515.55 141.31 145 40 47 7 167.81 0.79 146 40 78 38 167.81 0.79 147 41 44 3 817.76 1.91 148 41 223 182 172.41 2.98 149 41 228 187 167.93 0.76 150 46 219 173 226.41 1.42 151 46 230 184 226.97 1.35 152 46 246 200 116.42 0.91 153 46 247 201 106.25 0.09 154 47 78 31 489.06 47.73 155 47 228 181 227.19 1.30 156 47 229 182 226.99 1.35 157 47 230 183 226.99 1.35 158 48 77 29 489.13 47.73 159 48 78 30 489.16 47.72 160 48 79 31 489.18 47.71 161 48 219 171 227.03 1.36 162 48 230 182 227.00 1.35 163 48 232 184 226.95 1.34 164 48 236 188 208.98 0.91 165 48 243 195 123.48 1.99 166 48 246 198 115.75 0.60 167 48 247 199 104.41 0.90 168 49 78 29 489.18 47.71 169 49 79 30 489.16 47.70 170 49 80 31 489.15 47.70 171 49 81 32 489.20 47.66 172 49 208 159 260.72 15.24 173 49 229 180 227.65 1.26 174 49 230 181 227.11 1.33 175 49 231 182 226.98 1.35 176 49 232 183 226.99 1.34 177 50 73 23 489.19 47.77 178 50 77 27 489.20 47.73 179 50 79 29 489.34 47.64 180 50 80 30 489.25 47.65 181 50 81 31 489.43 47.56 182 50 232 182 227.03 1.35 183 50 236 186 212.28 1.05 184 51 69 18 489.59 47.63 185 51 81 30 489.43 47.56 186 51 83 32 489.98 47.28 187 51 110 59 313.98 39.06 188 51 231 180 227.68 1.24 189 51 232 181 227.19 1.29 190 51 233 182 226.90 1.32 191 51 237 186 209.43 0.69 192 52 64 12 490.54 47.19 193 52 65 13 490.49 47.21 194 52 66 14 489.65 47.59 195 52 69 17 489.67 47.59 196 52 81 29 489.97 47.31 197 52 82 30 489.91 47.31 198 52 83 31 489.99 47.27 199 52 95 43 318.30 38.49 200 52 237 185 214.81 0.21 201 53 56 3 898.68 3.68 202 53 60 7 491.86 46.70 203 53 83 30 489.99 47.27 204 53 89 36 464.50 33.16 205 53 233 180 227.63 1.26 206 53 234 181 227.57 1.26 207 53 237 184 219.96 0.08 208 54 61 7 491.77 46.70 209 54 62 8 491.48 46.78 210 54 66 12 490.56 47.18 211 54 90 36 464.50 33.16 212 54 91 37 463.95 33.43 213 54 95 41 321.22 37.57 214 54 237 183 225.58 0.71 215 55 60 5 491.86 46.70 216 55 61 6 491.78 46.70 217 55 87 32 491.20 46.27 218 55 88 33 490.45 45.93 219 55 89 34 464.61 33.20 220 55 90 35 464.54 33.16 221 56 59 3 826.58 3.81 222 56 60 4 491.90 46.69 223 56 88 32 490.62 46.00 224 56 89 33 471.20 36.35 225 57 88 31 490.63 46.00 226 57 89 32 490.63 46.00 227 57 187 130 269.25 19.51 228 58 182 124 270.46 18.87 229 58 187 129 269.16 19.53 230 60 234 174 227.82 1.21 231 60 237 177 227.59 1.25 232 60 238 178 227.43 1.32 233 62 237 175 226.98 1.29 234 62 238 176 226.79 1.28 235 64 69 5 805.64 2.16 236 64 237 173 226.25 1.00 237 64 238 174 226.19 1.03 238 64 240 176 223.34 1.71 239 65 68 3 831.88 3.37 240 65 69 4 805.72 2.17 241 66 69 3 831.83 3.35 242 66 70 4 826.50 2.60 243 66 95 29 320.57 39.77 244 66 99 33 320.58 39.80 245 67 70 3 829.90 4.30 246 67 71 4 829.90 4.30 247 67 98 31 320.61 39.80 248 67 99 32 320.59 39.80 249 67 102 35 320.53 40.09 250 68 71 3 834.82 1.84 251 68 72 4 811.58 2.38 252 69 72 3 832.15 1.39 253 69 73 4 828.48 3.21 254 69 236 167 226.19 1.03 255 69 237 168 225.34 0.71 256 69 240 171 145.25 12.00 257 70 73 3 832.15 1.40 258 70 74 4 828.50 3.21 259 70 80 10 492.53 46.13 260 70 99 29 320.67 39.86 261 70 103 33 319.88 40.44 262 71 74 3 836.62 0.84 263 71 75 4 836.85 3.74 264 71 102 31 320.53 40.22 265 71 103 32 320.40 40.22 266 72 75 3 844.97 1.89 267 72 76 4 843.07 2.83 268 72 240 168 149.03 10.71 269 72 241 169 124.42 1.59 270 72 243 171 123.51 1.99 271 72 244 172 123.50 2.00 272 73 76 3 843.17 2.79 273 73 77 4 805.32 0.65 274 73 80 7 680.42 47.81 275 73 236 163 225.34 0.71 276 73 240 167 124.41 1.59 277 73 243 170 123.48 1.99 278 74 103 29 483.55 122.28 279 74 104 30 486.45 123.76 280 74 105 31 479.99 120.86 281 76 243 167 123.48 1.98 282 76 244 168 123.46 1.97 283 76 247 171 107.94 0.68 284 77 105 28 503.99 133.32 285 78 106 28 503.69 133.46 286 79 105 26 503.82 133.53 287 79 106 27 503.68 133.46 288 80 99 19 505.91 132.55 289 80 103 23 504.98 133.03 290 80 105 25 504.38 133.31 291 80 106 26 503.93 133.50 292 80 107 27 503.55 133.60 293 80 108 28 503.54 133.60 294 81 108 27 503.54 133.60 295 81 110 29 503.16 133.70 296 81 231 150 227.63 1.26 297 82 95 13 506.10 132.50 298 82 96 14 505.91 132.50 299 82 99 17 506.04 132.53 300 82 107 25 504.01 133.48 301 82 108 26 503.97 133.47 302 82 109 27 503.45 133.66 303 82 110 28 503.21 133.67 304 83 89 6 651.36 60.26 305 83 91 8 645.56 62.93 306 83 92 9 645.54 62.94 307 83 110 27 503.35 133.75 308 84 91 7 651.12 60.17 309 84 92 8 645.54 62.94 310 84 93 9 645.54 62.95 311 84 96 12 506.82 132.25 312 84 109 25 503.63 133.65 313 84 110 26 503.50 133.69 314 84 111 27 503.35 133.74 315 85 88 3 867.45 1.91 316 85 89 4 709.12 31.43 317 85 90 5 651.50 60.24 318 85 91 6 651.40 60.23 319 85 111 26 503.36 133.76 320 85 112 27 503.34 133.77 321 86 90 4 651.49 60.23 322 86 91 5 651.43 60.22 323 86 92 6 651.40 60.23 324 89 112 23 503.40 133.80 325 92 95 3 837.75 0.82 326 92 96 4 808.98 3.12 327 93 96 3 830.69 4.34 328 93 97 4 808.98 3.12 329 93 109 16 504.06 133.47 330 93 121 28 272.14 18.70 331 93 125 32 272.62 18.41 332 94 97 3 821.80 3.29 333 94 98 4 808.98 3.12 334 95 98 3 840.03 1.49 335 95 99 4 803.87 0.58 336 96 99 3 828.38 3.88 337 96 100 4 803.75 0.63 338 96 107 11 504.67 133.22 339 96 109 13 503.90 133.55 340 96 125 29 272.62 18.41 341 96 127 31 273.41 17.59 342 97 100 3 825.74 1.32 343 97 101 4 809.25 2.68 344 97 125 28 272.61 18.41 345 98 101 3 823.71 2.40 346 98 102 4 811.69 1.47 347 99 102 3 819.21 0.18 348 99 103 4 817.08 1.24 349 99 107 8 504.76 133.22 350 100 103 3 837.58 3.03 351 100 107 7 504.75 133.16 352 100 125 25 338.09 14.33 353 100 126 26 321.49 7.27 354 103 107 4 846.18 1.35 355 106 128 22 349.17 20.76 356 107 127 20 349.73 20.55 357 107 128 21 349.33 20.72 358 107 129 22 349.24 20.73 359 108 129 21 349.51 20.62 360 108 130 22 349.49 20.61 361 108 131 23 349.19 20.76 362 108 157 49 348.99 20.84 363 108 231 123 259.41 17.15 364 109 113 4 891.96 3.63 365 109 118 9 351.72 21.00 366 109 122 13 350.57 20.54 367 109 127 18 350.41 20.62 368 109 129 20 349.96 20.67 369 109 130 21 349.61 20.61 370 109 131 22 349.95 20.78 371 110 131 21 349.90 20.81 372 110 210 100 348.89 20.87 373 110 231 121 260.76 17.64 374 111 131 20 350.36 20.66 375 111 133 22 351.31 21.16 376 112 118 6 779.31 10.61 377 112 133 21 351.32 21.15 378 112 136 24 351.51 21.18 379 112 184 72 351.22 21.11 380 113 118 5 649.40 54.34 381 113 119 6 778.90 10.47 382 113 130 17 351.56 21.09 383 113 131 18 351.42 21.16 384 113 132 19 351.38 21.15 385 113 137 24 351.56 21.20 386 113 145 32 352.08 21.00 387 114 118 4 831.08 5.66 388 114 136 22 351.53 21.18 389 114 137 23 351.54 21.18 390 114 145 31 352.13 21.00 391 115 118 3 844.45 2.62 392 115 145 30 352.48 21.12 393 116 119 3 839.39 1.51 394 116 120 4 820.95 1.96 395 116 145 29 352.49 21.12 396 116 148 32 352.58 21.12 397 117 120 3 833.62 4.38 398 117 121 4 817.58 1.20 399 118 121 3 819.82 0.08 400 118 122 4 818.26 0.88 401 119 122 3 819.84 0.09 402 119 123 4 818.26 0.88 403 119 130 11 352.50 21.19 404 119 145 26 402.09 2.06 405 119 148 29 402.25 2.04 406 119 149 30 402.08 2.06 407 119 152 33 497.94 45.66 408 120 123 3 819.85 0.09 409 120 124 4 818.26 0.88 410 120 148 28 460.09 26.09 411 120 152 32 512.38 47.64 412 121 124 3 825.39 2.69 413 121 125 4 819.12 0.45 414 122 125 3 825.39 2.69 415 122 126 4 819.05 0.41 416 122 127 5 514.87 46.41 417 122 130 8 399.92 1.42 418 122 154 32 612.39 2.35 419 123 126 3 825.32 2.73 420 123 152 29 573.82 16.94 421 123 154 31 573.81 16.95 422 127 154 27 714.46 21.07 423 128 154 26 714.46 21.07 424 129 154 25 714.46 21.06 425 129 155 26 714.46 21.07 426 129 156 27 714.43 21.07 427 130 149 19 714.75 21.01 428 130 154 24 714.69 21.00 429 130 156 26 714.43 21.07 430 130 157 27 714.43 21.07 431 131 157 26 714.44 21.07 432 131 159 28 714.41 21.07 433 132 142 10 714.89 21.02 434 132 145 13 714.89 21.02 435 132 146 14 714.88 21.02 436 132 156 24 714.65 21.01 437 132 157 25 714.56 21.04 438 132 158 26 714.41 21.07 439 132 159 27 714.41 21.07 440 133 136 3 841.27 0.57 441 133 137 4 805.00 5.38 442 133 159 26 714.42 21.07 443 133 184 51 497.08 6.86 444 134 137 3 825.73 2.44 445 134 142 8 714.93 21.00 446 134 158 24 714.60 21.05 447 134 159 25 714.46 21.08 448 134 160 26 714.42 21.07 449 134 165 31 655.16 26.00 450 134 168 34 654.06 25.53 451 134 169 35 653.90 25.46 452 135 161 26 714.40 21.05 453 135 165 30 667.07 30.54 454 135 183 48 604.59 29.19 455 135 184 49 552.46 16.48 456 137 141 4 806.15 5.75 457 137 142 5 780.78 6.82 458 137 145 8 722.12 19.79 459 138 141 3 831.15 4.30 460 138 142 4 814.14 1.76 461 139 142 3 833.07 1.18 462 139 143 4 818.48 0.41 463 139 168 29 654.07 25.51 464 139 171 32 653.99 25.47 465 139 172 33 653.72 25.47 466 140 143 3 836.12 2.71 467 140 144 4 822.99 1.84 468 141 144 3 831.64 2.83 469 141 145 4 822.99 1.84 470 142 145 3 843.89 1.26 471 142 146 4 822.97 1.84 472 142 158 16 714.68 21.02 473 142 168 26 654.07 25.51 474 142 172 30 653.55 25.46 475 143 146 3 835.90 5.29 476 143 147 4 822.11 1.61 477 143 172 29 653.53 25.46 478 144 147 3 833.99 0.48 479 144 148 4 816.07 1.41 480 145 148 3 817.80 0.56 481 145 149 4 816.35 1.28 482 146 149 3 839.53 2.29 483 146 150 4 819.15 0.13 484 146 156 10 714.82 21.02 485 146 158 12 714.78 21.02 486 146 172 26 653.51 25.45 487 147 150 3 821.39 1.24 488 147 151 4 819.15 0.13 489 148 151 3 825.25 1.86 490 148 152 4 822.99 1.79 491 149 152 3 840.78 1.52 492 149 153 4 811.86 1.03 493 149 154 5 768.99 4.36 494 149 156 7 715.00 20.95 495 150 153 3 814.90 0.48 496 150 154 4 785.26 3.77 497 150 155 5 769.72 4.00 498 150 156 6 716.92 21.67 499 150 173 23 653.45 25.44 500 150 175 25 653.13 25.34 501 155 175 20 653.06 25.32 502 155 176 21 653.00 25.34 503 156 176 20 653.01 25.34 504 157 176 19 653.00 25.34 505 157 177 20 652.96 25.36 506 157 178 21 652.88 25.37 507 157 208 51 491.04 6.15 508 157 231 74 261.28 17.93 509 158 169 11 653.50 25.46 510 158 172 14 653.54 25.46 511 158 174 16 653.15 25.36 512 158 176 18 653.13 25.34 513 158 177 19 652.97 25.37 514 158 178 20 652.95 25.36 515 159 178 19 652.88 25.41 516 159 180 21 652.79 25.38 517 159 184 25 534.54 0.73 518 159 210 51 490.94 6.18 519 160 165 5 655.15 26.00 520 160 166 6 655.14 26.00 521 160 169 9 654.08 25.52 522 160 177 17 653.04 25.41 523 160 178 18 652.99 25.40 524 160 179 19 652.87 25.40 525 160 180 20 652.86 25.39 526 160 197 37 495.92 6.60 527 160 201 41 492.98 6.67 528 161 165 4 811.10 1.79 529 161 180 19 652.87 25.39 530 161 182 21 652.72 25.42 531 161 183 22 635.07 22.73 532 161 193 32 496.06 6.65 533 161 197 36 495.97 6.61 534 162 165 3 823.24 2.42 535 162 166 4 815.25 0.17 536 162 197 35 495.98 6.62 537 163 166 3 821.83 3.12 538 163 167 4 811.11 1.79 539 163 196 33 496.06 6.65 540 163 197 34 495.99 6.62 541 163 200 37 495.81 6.62 542 164 167 3 827.66 6.04 543 164 168 4 814.78 0.05 544 165 168 3 824.87 4.50 545 165 169 4 825.32 4.76 546 166 169 3 825.33 4.76 547 166 170 4 825.33 4.76 548 166 177 11 653.31 25.42 549 166 197 31 495.97 6.62 550 166 200 34 495.20 6.92 551 166 201 35 492.98 6.67 552 167 170 3 825.35 4.77 553 167 171 4 825.33 4.75 554 168 171 3 844.00 4.57 555 168 172 4 825.34 4.76 556 169 172 3 833.56 0.66 557 169 173 4 815.33 0.24 558 169 174 5 722.56 24.45 559 169 177 8 653.16 25.36 560 170 173 3 825.37 2.16 561 170 177 7 653.43 25.37 562 170 202 32 491.51 6.12 563 174 177 3 879.27 3.61 564 175 205 30 491.20 6.10 565 175 206 31 491.20 6.10 566 176 206 30 491.16 6.12 567 176 208 32 491.10 6.14 568 177 201 24 491.50 6.10 569 177 205 28 491.32 6.09 570 177 206 29 491.25 6.08 571 177 207 30 491.10 6.14 572 177 208 31 491.10 6.14 573 178 208 30 491.06 6.15 574 178 210 32 490.97 6.19 575 179 194 15 495.86 6.61 576 179 197 18 495.86 6.61 577 179 198 19 495.30 6.88 578 179 201 22 492.88 6.71 579 179 207 28 491.31 6.09 580 179 208 29 491.25 6.08 581 179 209 30 491.06 6.16 582 179 210 31 490.98 6.19 583 180 184 4 846.38 4.08 584 180 210 30 490.96 6.19 585 180 211 31 490.96 6.19 586 181 189 8 496.12 6.66 587 181 190 9 496.08 6.65 588 181 191 10 495.99 6.63 589 181 194 13 495.92 6.60 590 181 210 29 491.24 6.06 591 181 211 30 491.00 6.17 592 182 187 5 506.03 2.03 593 182 188 6 496.55 6.74 594 182 189 7 496.12 6.66 595 182 211 29 490.94 6.18 596 183 186 3 844.18 2.53 597 183 187 4 506.06 2.02 598 183 188 5 496.55 6.74 599 184 187 3 801.98 14.45 600 189 211 22 491.24 6.06 601 189 212 23 491.00 6.16 602 190 193 3 840.87 4.04 603 190 194 4 812.33 3.31 604 191 194 3 812.32 3.31 605 191 195 4 812.32 3.31 606 191 212 21 491.26 6.07 607 191 218 27 439.63 1.20 608 192 195 3 823.42 2.23 609 192 196 4 807.03 0.67 610 193 196 3 820.05 5.96 611 193 197 4 821.87 4.29 612 194 197 3 831.86 0.06 613 194 198 4 821.87 4.29 614 194 209 15 491.35 6.08 615 194 210 16 491.34 6.08 616 195 198 3 834.20 1.47 617 195 199 4 821.88 4.29 618 195 218 23 436.63 2.50 619 195 221 26 306.88 19.67 620 195 222 27 289.43 17.91 621 195 225 30 265.55 19.39 622 196 199 3 835.00 2.28 623 196 200 4 821.88 4.28 624 197 200 3 833.99 2.79 625 197 201 4 820.87 3.77 626 198 201 3 836.14 3.87 627 198 207 9 491.48 6.09 628 198 222 24 265.39 19.41 629 198 225 27 265.31 19.41 630 198 227 29 262.24 17.93 631 201 205 4 829.12 3.32 632 201 207 6 493.55 5.07 633 202 205 3 851.51 1.03 634 202 207 5 822.33 3.85 635 203 207 4 855.43 0.98 636 206 228 22 261.76 18.03 637 207 226 19 262.48 17.99 638 207 227 20 261.88 18.06 639 207 228 21 261.83 18.05 640 207 229 22 261.76 18.03 641 208 227 19 262.23 17.89 642 208 229 21 261.66 18.01 643 208 230 22 261.62 18.00 644 208 231 23 261.61 17.99 645 209 213 4 854.03 1.53 646 209 222 13 262.56 18.04 647 209 227 18 262.27 17.88 648 209 229 20 262.05 17.98 649 209 230 21 261.64 18.00 650 209 231 22 261.62 18.00 651 210 231 21 261.61 17.99 652 213 218 5 776.79 5.42 653 213 219 6 648.74 70.86 654 213 222 9 403.07 41.12 655 213 230 17 262.30 17.87 656 213 232 19 261.89 17.86 657 213 235 22 261.60 17.99 658 214 235 21 261.60 17.99 659 214 242 28 125.62 2.07 660 214 246 32 123.50 2.00 661 215 218 3 831.73 3.17 662 215 219 4 825.89 6.09 663 215 242 27 130.10 0.17 664 215 246 31 123.51 2.00 665 216 219 3 834.36 1.85 666 216 220 4 819.88 2.20 667 216 245 29 123.54 1.99 668 216 246 30 123.48 1.99 669 216 248 32 123.45 1.97 670 217 220 3 831.90 3.08 671 217 221 4 819.93 2.22 672 218 221 3 840.95 5.15 673 218 222 4 813.25 5.55 674 219 222 3 829.44 1.11 675 219 223 4 826.59 1.15 676 219 230 11 262.60 17.91 677 219 232 13 262.58 17.91 678 219 246 27 123.13 1.84 679 220 223 3 830.46 0.29 680 220 224 4 829.81 0.45 681 221 224 3 830.94 0.52 682 221 225 4 829.81 0.45 683 222 225 3 829.81 0.44 684 222 226 4 829.81 0.44 685 222 227 5 782.97 6.71 686 222 230 8 325.85 2.78 687 223 226 3 832.81 0.70 688 223 227 4 804.44 3.79 689 223 228 5 791.93 2.46 690 223 230 7 778.44 5.21 691 227 230 3 934.39 0.56 692 232 235 3 891.24 0.90 693 232 236 4 816.80 7.05 694 232 246 14 122.55 1.84 695 233 236 3 836.50 0.23 696 233 237 4 806.45 10.06 697 234 237 3 836.49 0.23 698 234 238 4 814.63 5.96 699 235 238 3 831.25 2.35 700 235 239 4 782.81 3.45 701 235 246 11 264.52 18.55 702 236 239 3 815.64 4.71 703 236 240 4 706.93 39.74 704 236 242 6 561.10 114.28 705 236 243 7 280.89 25.82 706 236 246 10 265.45 18.08 707 239 242 3 842.39 2.72 708 240 243 3 844.62 0.68 709 241 244 3 848.82 6.22 710 242 245 3 831.27 0.25 711 242 246 4 821.30 4.74 712 243 246 3 821.35 4.71 713 243 247 4 813.87 1.35 714 244 247 3 827.05 0.96 715 244 248 4 818.28 2.81 716 245 248 3 839.92 5.99