BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 116.34 0.15 2 3 228 225 115.05 0.15 3 3 229 226 113.42 0.95 4 4 204 200 179.59 1.65 5 4 205 201 179.19 1.51 6 4 206 202 176.49 0.16 7 4 207 203 171.00 1.66 8 4 229 225 116.44 0.20 9 5 36 31 491.43 93.25 10 5 37 32 491.40 93.27 11 5 38 33 491.38 93.28 12 5 207 202 179.19 1.51 13 6 36 30 491.50 93.23 14 6 37 31 491.41 93.27 15 6 38 32 491.41 93.27 16 6 223 217 116.62 0.24 17 6 229 223 116.58 0.24 18 6 249 243 20.45 0.40 19 7 37 30 491.49 93.24 20 7 38 31 491.42 93.27 21 7 40 33 491.47 93.23 22 7 90 83 203.48 0.07 23 7 163 156 184.40 1.38 24 7 207 200 183.56 1.14 25 7 209 202 176.08 0.05 26 7 229 222 118.00 0.65 27 7 230 223 116.59 0.24 28 7 231 224 116.50 0.20 29 8 37 29 491.54 93.23 30 8 38 30 491.50 93.25 31 8 39 31 491.42 93.26 32 8 40 32 491.48 93.23 33 8 231 223 116.70 0.19 34 8 242 234 51.49 0.82 35 8 246 238 37.07 0.21 36 9 40 31 491.48 93.23 37 9 124 115 199.89 0.53 38 9 231 222 117.70 0.64 39 9 232 223 116.51 0.19 40 9 233 224 116.48 0.19 41 9 242 233 52.38 0.41 42 10 40 30 491.53 93.23 43 10 41 31 491.52 93.21 44 10 42 32 491.56 93.19 45 10 233 223 116.55 0.17 46 10 237 227 86.11 0.12 47 10 242 232 52.38 0.41 48 11 42 31 491.57 93.20 49 11 65 54 305.42 0.84 50 11 92 81 203.53 0.01 51 11 95 84 202.86 0.07 52 11 232 221 120.72 0.02 53 11 233 222 117.62 0.60 54 11 234 223 116.50 0.18 55 11 237 226 89.30 0.63 56 12 15 3 876.38 3.60 57 12 21 9 495.06 91.73 58 12 24 12 492.24 93.09 59 12 25 13 492.21 93.11 60 12 28 16 492.20 93.09 61 12 41 29 491.60 93.20 62 12 42 30 491.59 93.19 63 12 43 31 491.56 93.20 64 12 51 39 455.75 75.13 65 12 65 53 306.08 1.03 66 12 237 225 115.45 0.18 67 12 238 226 89.05 0.59 68 13 44 31 491.59 93.19 69 13 65 52 305.58 0.92 70 13 95 82 203.70 0.05 71 13 97 84 203.06 0.15 72 13 172 159 202.90 0.10 73 13 234 221 120.77 0.02 74 14 44 30 491.62 93.18 75 15 44 29 491.63 93.18 76 15 238 223 116.43 0.20 77 16 20 4 843.97 0.87 78 16 21 5 825.50 4.03 79 16 47 31 491.69 92.99 80 17 20 3 866.22 4.86 81 17 21 4 842.35 1.73 82 18 21 3 871.75 2.08 83 18 22 4 844.57 2.83 84 18 50 32 482.81 88.39 85 19 22 3 875.58 3.98 86 19 23 4 856.95 0.35 87 20 23 3 870.98 1.67 88 20 24 4 865.20 6.42 89 21 24 3 874.88 1.57 90 21 25 4 853.45 0.53 91 21 47 26 491.05 92.67 92 21 50 29 458.38 75.90 93 21 51 30 458.24 75.96 94 22 25 3 874.88 1.57 95 22 26 4 863.18 4.01 96 22 50 28 458.32 75.87 97 22 54 32 445.55 69.44 98 23 26 3 869.43 0.90 99 23 27 4 863.19 4.01 100 24 27 3 865.34 2.94 101 24 28 4 862.77 4.22 102 24 237 213 112.01 0.16 103 24 238 214 100.84 0.07 104 24 239 215 94.44 3.27 105 24 240 216 87.13 0.38 106 25 28 3 868.70 4.62 107 25 29 4 853.37 0.48 108 25 51 26 456.67 74.75 109 25 54 29 308.41 0.57 110 25 55 30 308.32 0.54 111 26 29 3 854.82 1.51 112 26 30 4 841.00 0.43 113 27 30 3 864.44 2.08 114 27 31 4 844.37 1.26 115 27 240 213 82.75 1.45 116 27 243 216 62.90 1.45 117 28 31 3 864.48 4.91 118 28 55 27 308.56 0.42 119 28 57 29 308.36 0.29 120 28 60 32 308.29 0.27 121 28 237 209 100.97 0.09 122 28 242 214 60.26 3.38 123 29 54 25 308.63 0.46 124 29 55 26 308.56 0.42 125 29 56 27 308.54 0.40 126 29 57 28 308.33 0.30 127 31 57 26 309.68 0.42 128 31 243 212 51.52 0.80 129 31 246 215 51.55 0.81 130 31 247 216 50.89 0.48 131 33 37 4 867.79 2.27 132 33 39 6 584.00 47.12 133 33 57 24 427.74 59.45 134 33 59 26 387.59 39.41 135 33 60 27 396.09 43.66 136 33 246 213 50.47 0.40 137 35 59 24 442.63 66.97 138 35 246 211 50.46 0.40 139 35 247 212 44.51 1.03 140 35 249 214 36.52 0.40 141 35 250 215 26.57 1.19 142 37 59 22 442.50 67.07 143 37 246 209 38.37 0.39 144 37 249 212 29.95 0.18 145 38 59 21 442.50 67.07 146 38 60 22 442.48 67.08 147 38 61 23 442.47 67.08 148 38 90 52 203.38 0.12 149 39 59 20 442.53 67.07 150 39 60 21 442.48 67.08 151 39 61 22 442.48 67.08 152 39 246 207 38.73 1.08 153 40 61 21 442.48 67.08 154 40 63 23 442.40 67.06 155 40 90 50 203.72 0.04 156 40 124 84 196.32 0.57 157 41 51 10 580.73 1.87 158 41 55 14 443.19 66.90 159 41 60 19 442.76 67.05 160 41 61 20 442.64 67.09 161 41 62 21 442.46 67.09 162 41 63 22 442.45 67.09 163 42 63 21 442.45 67.09 164 42 65 23 441.71 67.32 165 42 92 50 203.75 0.06 166 43 47 4 861.99 3.18 167 43 48 5 763.58 38.40 168 43 51 8 628.28 25.54 169 43 62 19 442.76 67.05 170 43 63 20 442.69 67.07 171 43 65 22 442.38 67.12 172 44 47 3 879.46 0.79 173 44 48 4 866.18 1.16 174 44 65 21 442.42 67.11 175 45 48 3 885.50 2.24 176 45 49 4 896.18 1.47 177 45 64 19 442.70 67.08 178 45 66 21 442.58 67.09 179 45 78 33 210.28 0.08 180 46 49 3 927.73 0.76 181 47 50 3 894.55 2.29 182 47 51 4 858.45 2.31 183 48 51 3 875.12 4.64 184 48 52 4 853.35 4.86 185 48 62 14 442.93 67.03 186 48 64 16 442.77 67.05 187 48 83 35 206.40 0.33 188 48 86 38 206.31 0.33 189 48 88 40 205.97 0.21 190 49 52 3 863.73 2.28 191 49 53 4 847.77 2.06 192 50 53 3 879.10 4.30 193 50 54 4 861.16 3.57 194 51 54 3 870.03 0.23 195 51 55 4 854.81 6.75 196 51 62 11 442.94 67.05 197 52 62 10 442.97 67.03 198 52 86 34 206.23 0.32 199 52 87 35 206.11 0.28 200 52 88 36 205.94 0.19 201 53 56 3 1054.88 0.48 202 55 60 5 443.11 67.03 203 55 62 7 442.96 67.06 204 57 60 3 875.76 3.74 205 60 242 182 58.40 2.57 206 60 246 186 44.61 1.01 207 61 90 29 203.38 0.12 208 62 88 26 204.82 0.28 209 62 90 28 203.91 0.02 210 63 88 25 205.02 0.19 211 63 90 27 203.77 0.05 212 63 91 28 203.81 0.03 213 63 92 29 204.09 0.07 214 64 83 19 206.32 0.18 215 64 88 24 205.84 0.14 216 64 90 26 204.47 0.10 217 64 91 27 204.18 0.08 218 64 92 28 204.29 0.03 219 65 92 27 204.29 0.02 220 66 91 25 205.65 0.10 221 66 92 26 205.03 0.17 222 66 93 27 205.00 0.18 223 66 98 32 204.98 0.18 224 66 112 46 206.34 0.18 225 67 78 11 211.38 0.06 226 67 80 13 210.61 0.11 227 67 83 16 210.34 0.29 228 67 91 24 205.93 0.06 229 67 93 26 205.75 0.14 230 67 112 45 206.34 0.18 231 68 74 6 262.13 23.50 232 68 77 9 212.68 0.04 233 68 78 10 212.61 0.07 234 68 79 11 211.19 0.14 235 68 80 12 210.94 0.01 236 68 112 44 207.00 0.47 237 69 80 11 211.86 0.18 238 69 81 12 212.69 0.03 239 69 115 46 206.86 0.30 240 69 119 50 211.49 0.01 241 70 74 4 905.57 3.83 242 70 79 9 212.69 0.03 243 70 81 11 212.73 0.01 244 70 119 49 212.68 0.01 245 71 74 3 974.27 2.65 246 71 79 8 212.75 0.01 247 71 81 10 212.75 0.01 248 71 82 11 212.76 0.01 249 74 77 3 882.19 6.17 250 78 82 4 863.97 6.66 251 78 83 5 851.76 0.55 252 79 82 3 871.86 2.71 253 79 83 4 851.79 0.56 254 80 83 3 863.78 1.33 255 80 84 4 851.82 0.55 256 80 93 13 472.04 9.27 257 80 112 32 527.90 28.24 258 80 115 35 528.16 28.36 259 80 116 36 526.71 28.84 260 80 119 39 528.47 28.46 261 80 121 41 527.20 29.00 262 81 84 3 862.58 1.47 263 81 85 4 851.84 0.53 264 81 119 38 536.48 30.51 265 82 85 3 853.51 0.77 266 82 86 4 848.33 1.22 267 83 86 3 875.43 4.96 268 83 87 4 848.33 1.22 269 83 88 5 821.81 1.44 270 83 121 38 564.16 24.34 271 84 87 3 873.13 6.10 272 84 88 4 825.16 3.12 273 84 89 5 821.80 1.44 274 84 119 35 564.50 24.29 275 84 120 36 564.40 24.34 276 84 121 37 564.26 24.33 277 88 121 33 603.24 29.00 278 89 121 32 603.22 28.99 279 89 122 33 603.22 28.99 280 90 121 31 603.26 29.00 281 90 122 32 603.23 29.00 282 90 124 34 603.20 28.98 283 90 163 73 603.22 28.98 284 91 116 25 603.42 28.96 285 91 121 30 603.39 28.95 286 91 122 31 603.32 28.96 287 91 123 32 603.25 28.99 288 91 124 33 603.22 28.98 289 92 95 3 921.86 1.38 290 92 124 32 603.24 28.99 291 92 126 34 603.19 28.98 292 92 232 140 126.38 0.20 293 93 112 19 603.49 28.95 294 93 113 20 603.45 28.95 295 93 116 23 603.50 28.96 296 93 123 30 603.38 28.96 297 93 124 31 603.33 28.98 298 93 125 32 603.25 28.98 299 93 126 33 603.20 28.97 300 94 99 5 818.92 0.79 301 94 109 15 603.43 28.96 302 94 126 32 603.25 28.99 303 95 98 3 869.21 1.45 304 95 99 4 837.37 6.25 305 95 126 31 603.28 28.98 306 95 167 72 603.13 28.97 307 96 99 3 846.68 1.59 308 96 100 4 837.38 6.25 309 96 169 73 588.46 34.83 310 96 172 76 477.56 0.25 311 97 100 3 865.41 0.34 312 97 101 4 836.00 6.93 313 97 102 5 819.73 1.18 314 97 172 75 542.92 24.72 315 98 102 4 706.42 57.80 316 98 103 5 624.46 32.82 317 98 104 6 609.07 26.25 318 99 104 5 603.69 28.93 319 99 106 7 604.10 28.73 320 99 109 10 603.71 28.94 321 99 126 27 603.47 28.94 322 99 127 28 603.44 28.94 323 99 146 47 603.52 28.96 324 103 108 5 849.32 2.79 325 104 108 4 849.32 2.79 326 104 109 5 845.00 0.66 327 104 112 8 824.69 4.33 328 105 108 3 874.09 0.32 329 105 109 4 849.32 2.79 330 106 109 3 865.44 3.96 331 106 110 4 864.13 4.60 332 106 146 40 603.68 28.96 333 106 149 43 604.20 28.74 334 107 110 3 875.21 0.93 335 107 111 4 863.93 4.49 336 107 153 46 605.48 28.70 337 108 111 3 864.14 4.60 338 108 112 4 863.93 4.49 339 109 112 3 864.12 4.58 340 109 113 4 850.73 2.11 341 109 150 41 604.33 28.80 342 110 113 3 873.38 0.05 343 110 114 4 850.77 2.08 344 110 149 39 604.69 28.62 345 110 150 40 604.33 28.80 346 110 153 43 605.64 28.62 347 111 114 3 877.35 1.94 348 111 115 4 846.54 0.04 349 112 115 3 853.53 1.50 350 112 116 4 846.51 0.05 351 113 116 3 860.43 0.43 352 113 117 4 840.96 2.79 353 113 123 10 605.25 28.80 354 113 125 12 604.72 28.61 355 113 150 37 605.25 28.80 356 113 154 41 606.24 28.77 357 113 162 49 605.20 28.65 358 114 117 3 856.64 3.80 359 114 118 4 848.32 6.46 360 114 153 39 606.19 28.75 361 114 154 40 606.19 28.75 362 115 118 3 852.05 4.61 363 115 119 4 848.31 6.48 364 116 119 3 862.64 0.69 365 116 120 4 852.20 4.53 366 116 121 5 680.00 64.31 367 116 123 7 611.68 31.52 368 117 120 3 848.65 6.30 369 117 123 6 614.89 33.02 370 117 154 37 714.85 2.61 371 117 158 41 698.71 8.95 372 117 161 44 695.83 6.84 373 117 162 45 692.16 8.67 374 120 161 41 807.73 1.61 375 121 161 40 807.74 1.61 376 122 160 38 807.88 1.61 377 122 161 39 807.73 1.61 378 122 163 41 807.75 1.62 379 122 207 85 403.76 8.51 380 123 161 38 807.86 1.60 381 123 162 39 807.76 1.62 382 123 163 40 807.75 1.62 383 124 163 39 807.75 1.62 384 124 165 41 807.71 1.64 385 124 232 108 126.38 0.20 386 125 150 25 808.02 1.60 387 125 163 38 807.86 1.60 388 125 164 39 807.77 1.62 389 125 165 40 807.77 1.62 390 126 165 39 807.78 1.61 391 126 232 106 126.78 0.27 392 127 143 16 808.26 1.59 393 127 146 19 808.22 1.61 394 127 147 20 808.19 1.60 395 127 150 23 808.20 1.60 396 127 165 38 807.84 1.61 397 127 166 39 807.78 1.61 398 127 167 40 807.79 1.61 399 127 190 63 415.84 10.92 400 128 143 15 808.27 1.59 401 128 146 18 808.27 1.59 402 128 166 38 807.84 1.61 403 128 167 39 807.80 1.61 404 128 168 40 807.73 1.62 405 129 133 4 823.07 2.56 406 129 134 5 809.83 1.67 407 129 139 10 808.38 1.57 408 129 142 13 808.37 1.58 409 129 166 37 807.90 1.61 410 129 168 39 807.80 1.61 411 129 169 40 807.76 1.61 412 129 170 41 807.75 1.61 413 130 133 3 857.11 2.21 414 130 134 4 840.25 5.62 415 130 169 39 807.78 1.60 416 130 170 40 807.76 1.61 417 131 134 3 850.66 6.54 418 131 135 4 840.27 5.61 419 131 170 39 807.79 1.60 420 132 135 3 861.12 1.49 421 132 136 4 847.62 2.92 422 133 136 3 854.35 0.44 423 133 137 4 847.61 2.92 424 133 138 5 808.71 1.62 425 133 139 6 808.81 1.56 426 133 142 9 808.68 1.63 427 134 137 3 862.62 3.70 428 134 139 5 808.56 1.57 429 134 166 32 808.00 1.60 430 134 170 36 807.81 1.61 431 134 182 48 417.14 10.70 432 134 186 52 416.97 10.74 433 138 141 3 863.45 4.21 434 138 142 4 837.25 3.87 435 139 142 3 868.14 1.85 436 139 143 4 849.26 2.34 437 139 166 27 807.94 1.62 438 139 186 47 416.44 10.77 439 140 143 3 867.91 1.73 440 140 144 4 858.73 1.48 441 140 189 49 416.39 10.79 442 141 144 3 867.89 1.73 443 141 145 4 858.50 1.36 444 142 145 3 866.38 2.50 445 142 146 4 853.13 4.04 446 143 146 3 874.29 6.44 447 143 147 4 859.36 2.26 448 143 166 23 807.87 1.61 449 143 186 43 416.37 10.79 450 143 189 46 416.36 10.79 451 143 190 47 416.02 10.90 452 144 147 3 862.99 0.79 453 144 148 4 849.68 2.57 454 144 189 45 416.35 10.79 455 144 190 46 416.03 10.90 456 144 193 49 414.84 10.81 457 145 148 3 872.14 0.51 458 145 149 4 849.81 2.63 459 146 149 3 868.91 1.51 460 146 150 4 863.10 4.01 461 147 150 3 868.67 1.26 462 147 151 4 862.99 4.09 463 147 159 12 808.26 1.60 464 147 164 17 807.91 1.62 465 147 190 43 415.83 10.92 466 147 193 46 413.36 10.48 467 148 151 3 862.90 4.36 468 148 152 4 862.68 4.25 469 148 193 45 413.36 10.48 470 149 152 3 874.11 0.10 471 150 153 3 881.48 6.17 472 150 154 4 864.18 0.27 473 151 154 3 868.18 1.73 474 151 159 8 808.81 1.39 475 151 193 42 413.34 10.47 476 151 198 47 405.61 8.67 477 154 158 4 859.83 2.15 478 154 159 5 851.12 2.19 479 154 162 8 808.53 1.75 480 155 158 3 872.58 3.57 481 155 159 4 864.81 0.33 482 156 159 3 879.08 4.68 483 156 198 42 405.62 8.68 484 156 199 43 405.62 8.68 485 156 201 45 405.60 8.68 486 156 202 46 405.52 8.65 487 157 160 3 883.10 2.73 488 158 161 3 864.81 0.33 489 158 162 4 863.22 0.47 490 159 162 3 857.02 0.52 491 159 194 35 412.01 10.58 492 159 198 39 405.62 8.67 493 159 202 43 405.29 8.54 494 159 206 47 404.41 8.55 495 162 206 44 403.99 8.43 496 163 206 43 404.01 8.43 497 163 207 44 403.76 8.51 498 163 209 46 403.57 8.48 499 164 206 42 404.60 8.47 500 164 207 43 403.81 8.51 501 164 208 44 403.66 8.52 502 164 209 45 403.63 8.51 503 165 209 44 403.22 8.30 504 165 232 67 126.89 0.46 505 166 186 20 415.92 10.99 506 166 187 21 415.62 10.85 507 166 190 24 415.80 10.94 508 166 209 43 403.79 8.51 509 166 210 44 403.59 8.48 510 166 211 45 403.13 8.28 511 167 172 5 584.44 3.93 512 167 211 44 403.07 8.31 513 167 232 65 127.70 0.15 514 168 171 3 862.05 3.70 515 168 172 4 842.83 0.23 516 168 178 10 416.95 10.72 517 168 210 42 403.83 8.50 518 168 211 43 403.77 8.51 519 169 172 3 876.85 3.70 520 170 174 4 852.50 5.62 521 170 176 6 417.42 10.59 522 170 178 8 417.17 10.71 523 170 182 12 417.16 10.71 524 171 174 3 866.38 1.20 525 171 176 5 417.16 10.71 526 171 177 6 417.12 10.69 527 171 210 39 404.34 8.39 528 171 212 41 402.76 8.15 529 171 213 42 402.76 8.15 530 172 212 40 402.99 8.26 531 174 213 39 403.19 8.29 532 175 213 38 403.19 8.29 533 176 213 37 403.22 8.29 534 177 210 33 404.62 8.48 535 177 212 35 403.77 8.50 536 177 213 36 403.73 8.48 537 177 218 41 361.35 5.92 538 177 222 45 286.19 28.64 539 178 182 4 830.77 5.85 540 178 183 5 817.92 0.66 541 178 210 32 404.63 8.48 542 178 222 44 285.70 28.56 543 179 182 3 862.55 1.27 544 179 183 4 861.08 0.54 545 180 183 3 862.56 1.27 546 180 184 4 847.20 7.48 547 180 221 41 182.00 22.97 548 180 222 42 182.00 22.97 549 180 225 45 133.96 0.16 550 181 184 3 870.26 5.25 551 181 185 4 860.57 0.80 552 181 225 44 178.81 22.58 553 182 185 3 878.83 0.96 554 182 186 4 860.84 0.93 555 183 186 3 878.82 0.97 556 183 187 4 847.49 7.61 557 183 210 27 404.72 8.53 558 183 222 39 180.43 22.18 559 183 228 45 128.44 0.13 560 184 187 3 859.81 1.45 561 184 188 4 848.38 7.16 562 184 225 41 128.43 0.14 563 184 226 42 128.44 0.14 564 184 227 43 128.34 0.18 565 184 228 44 128.33 0.18 566 185 188 3 865.50 5.65 567 185 189 4 851.83 5.44 568 186 189 3 865.49 5.65 569 186 190 4 845.44 4.01 570 187 190 3 864.50 0.32 571 187 191 4 856.46 4.34 572 187 208 21 404.84 8.53 573 187 209 22 404.83 8.53 574 187 210 23 404.72 8.54 575 187 227 40 128.13 0.21 576 187 228 41 128.12 0.21 577 188 191 3 864.51 0.32 578 188 192 4 864.50 0.32 579 188 227 39 128.14 0.21 580 189 192 3 864.52 0.32 581 189 193 4 859.34 2.91 582 190 193 3 866.73 0.78 583 190 194 4 859.34 2.91 584 190 208 18 404.84 8.53 585 191 194 3 870.00 2.83 586 191 195 4 857.89 2.19 587 191 203 12 405.64 8.64 588 191 206 15 405.37 8.55 589 191 208 17 404.85 8.52 590 191 227 36 128.12 0.21 591 191 229 38 128.09 0.21 592 192 195 3 859.44 2.96 593 192 196 4 852.15 0.69 594 193 196 3 869.31 3.66 595 193 197 4 841.89 4.45 596 193 198 5 841.89 4.45 597 194 197 3 869.31 3.66 598 194 198 4 758.96 31.95 599 194 199 5 755.99 31.80 600 194 202 8 418.99 2.31 601 194 203 9 405.84 8.73 602 194 206 12 405.19 8.44 603 195 199 4 540.17 15.34 604 195 200 5 425.92 10.83 605 195 203 8 405.86 8.73 606 198 202 4 837.52 4.66 607 199 202 3 860.22 6.91 608 199 203 4 842.32 3.58 609 200 203 3 850.94 7.33 610 200 204 4 842.32 3.58 611 201 204 3 863.50 4.58 612 201 205 4 856.75 4.46 613 202 205 3 874.79 1.06 614 202 206 4 844.31 1.76 615 203 206 3 866.84 8.92 616 206 229 23 127.92 0.21 617 207 229 22 127.78 0.18 618 208 228 20 128.02 0.20 619 208 229 21 127.95 0.20 620 209 229 20 127.96 0.20 621 209 230 21 127.77 0.18 622 209 231 22 127.72 0.16 623 209 232 23 127.70 0.15 624 210 214 4 872.58 0.08 625 210 222 12 127.92 0.21 626 210 228 18 128.06 0.21 627 210 230 20 127.96 0.20 628 210 231 21 127.72 0.16 629 210 232 22 127.72 0.16 630 211 214 3 897.59 2.27 631 211 232 21 127.67 0.14 632 213 218 5 845.73 1.21 633 214 218 4 835.17 4.07 634 214 219 5 451.09 21.77 635 214 222 8 374.76 15.64 636 214 231 17 128.03 0.18 637 214 233 19 127.90 0.20 638 214 236 22 127.09 0.42 639 214 245 31 51.35 0.74 640 215 218 3 866.31 0.42 641 215 219 4 664.20 84.78 642 215 236 21 127.66 0.14 643 215 245 30 52.05 0.40 644 216 241 25 52.52 0.30 645 216 244 28 52.18 0.47 646 216 245 29 51.48 0.81 647 218 221 3 870.75 0.63 648 218 222 4 858.79 0.88 649 219 222 3 863.26 3.11 650 219 223 4 845.98 1.07 651 219 231 12 128.35 0.09 652 219 245 26 49.61 0.83 653 219 248 29 38.36 0.84 654 220 223 3 859.85 0.88 655 220 224 4 845.98 1.07 656 220 248 28 38.30 0.87 657 221 224 3 861.65 0.95 658 222 225 3 879.84 6.34 659 222 228 6 129.82 0.69 660 222 231 9 128.52 0.15 661 223 228 5 129.82 0.69 662 223 229 6 129.81 0.69 663 223 230 7 128.53 0.14 664 223 231 8 128.53 0.15 665 223 248 25 36.95 0.24 666 223 249 26 27.02 1.29 667 225 228 3 880.10 6.17 668 228 231 3 1063.81 3.37 669 230 249 19 19.56 0.04 670 231 245 14 49.46 0.76 671 231 249 18 23.91 0.14 672 233 236 3 894.74 3.21 673 233 237 4 835.07 7.53 674 233 242 9 52.54 0.32 675 233 245 12 51.08 0.10 676 234 237 3 874.24 7.52 677 235 238 3 879.98 6.07 678 235 239 4 874.89 7.19 679 236 239 3 871.35 6.33 680 236 242 6 142.21 44.51 681 236 245 9 53.51 0.17 682 237 242 5 142.21 44.51 683 239 242 3 880.50 4.92 684 240 243 3 877.84 3.52 685 241 244 3 862.28 2.67 686 241 245 4 862.28 2.68 687 242 245 3 862.28 2.68 688 242 246 4 852.25 2.34 689 243 246 3 859.85 0.26 690 243 247 4 852.24 2.33 691 244 247 3 852.86 2.03 692 244 248 4 862.96 2.64 693 245 248 3 864.51 1.86 694 245 249 4 864.51 1.86 695 246 249 3 881.50 2.69 696 247 250 3 881.50 2.69