BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 20 168 148 109.58 1.64 2 21 48 27 127.64 0.50 3 21 49 28 127.66 0.50 4 21 50 29 127.67 0.51 5 21 51 30 127.66 0.50 6 22 42 20 127.66 0.50 7 22 51 29 127.66 0.50 8 22 52 30 127.69 0.52 9 22 53 31 127.67 0.50 10 22 56 34 127.66 0.49 11 22 59 37 127.62 0.52 12 22 164 142 127.65 0.51 13 23 51 28 127.80 0.48 14 23 52 29 127.71 0.51 15 23 53 30 127.71 0.51 16 24 53 29 127.72 0.52 17 24 54 30 127.72 0.52 18 24 55 31 127.69 0.50 19 24 56 32 127.69 0.50 20 25 29 4 826.40 0.33 21 25 53 28 127.81 0.48 22 25 54 29 127.73 0.52 23 26 29 3 826.40 0.33 24 28 31 3 832.69 0.05 25 28 32 4 807.91 2.27 26 28 41 13 128.14 0.55 27 28 43 15 137.52 1.29 28 28 52 24 137.76 1.31 29 28 53 25 134.64 0.14 30 28 54 26 128.38 0.40 31 28 257 229 50.61 1.61 32 28 261 233 40.27 1.40 33 29 32 3 830.06 0.96 34 29 33 4 801.19 0.95 35 29 54 25 130.40 0.18 36 30 33 3 821.16 3.48 37 30 34 4 793.08 3.10 38 31 35 4 807.25 1.32 39 31 253 222 66.06 1.27 40 31 256 225 57.58 0.07 41 31 257 226 56.25 0.04 42 32 35 3 831.46 5.41 43 32 36 4 734.31 29.62 44 32 37 5 622.41 10.60 45 32 39 7 573.19 27.39 46 32 40 8 384.09 121.93 47 32 41 9 138.72 1.76 48 32 54 22 142.25 0.21 49 32 237 205 137.85 1.33 50 32 253 221 68.86 0.14 51 32 257 225 57.41 0.01 52 33 36 3 731.78 28.35 53 35 249 214 72.05 0.78 54 35 253 218 71.61 0.61 55 37 239 202 138.88 1.59 56 37 249 212 72.11 0.81 57 37 253 216 71.43 0.53 58 38 58 20 149.66 1.35 59 38 239 201 142.13 0.02 60 38 240 202 137.76 1.42 61 39 54 15 177.94 3.06 62 39 58 19 149.66 1.35 63 39 238 199 146.09 0.26 64 39 239 200 142.39 0.10 65 40 53 13 177.98 3.06 66 40 54 14 177.95 3.06 67 40 55 15 177.66 3.15 68 40 58 18 163.96 3.06 69 40 59 19 161.11 0.58 70 40 163 123 159.97 3.78 71 40 237 197 157.30 2.48 72 40 238 198 148.92 1.64 73 41 53 12 178.00 3.08 74 41 54 13 177.96 3.06 75 41 235 194 177.62 3.10 76 41 236 195 177.44 3.04 77 41 237 196 157.58 2.62 78 41 254 213 56.73 0.35 79 41 257 216 45.86 0.09 80 41 258 217 45.77 0.06 81 42 51 9 866.02 2.01 82 42 52 10 730.20 27.43 83 42 53 11 339.61 81.53 84 42 59 17 177.74 3.11 85 42 163 121 177.66 3.12 86 42 164 122 177.69 3.14 87 42 167 125 177.74 3.10 88 42 235 193 177.65 3.12 89 42 236 194 177.59 3.09 90 43 51 8 1015.33 0.35 91 43 52 9 850.84 9.61 92 43 233 190 193.93 0.70 93 43 234 191 190.18 1.17 94 43 235 192 178.59 2.69 95 43 258 215 45.43 0.07 96 43 261 218 33.11 0.34 97 43 263 220 32.70 0.20 98 44 167 123 193.94 0.70 99 44 233 189 197.68 0.92 100 44 234 190 193.91 0.70 101 45 231 186 213.04 0.76 102 45 232 187 212.97 0.74 103 45 233 188 206.49 2.10 104 46 231 185 213.06 0.76 105 46 232 186 212.98 0.73 106 46 234 188 212.97 0.74 107 47 171 124 213.03 0.75 108 47 234 187 213.02 0.76 109 48 167 119 212.91 0.70 110 48 168 120 213.03 0.76 111 48 171 123 213.09 0.78 112 51 167 116 212.68 0.66 113 53 56 3 842.82 2.79 114 53 59 6 705.41 22.68 115 55 58 3 836.05 2.33 116 55 59 4 796.17 0.82 117 56 59 3 829.25 1.06 118 56 60 4 821.71 2.71 119 57 60 3 832.73 0.68 120 57 61 4 815.49 1.83 121 58 61 3 819.49 4.65 122 58 63 5 819.49 4.65 123 58 238 180 213.15 0.78 124 58 239 181 213.10 0.76 125 58 240 182 213.08 0.76 126 59 162 103 357.17 21.77 127 59 163 104 315.94 23.73 128 59 164 105 315.95 23.73 129 60 162 102 366.79 26.45 130 60 164 104 357.18 21.77 131 61 162 101 376.11 28.59 132 62 143 81 414.39 27.17 133 62 160 98 414.39 27.17 134 62 161 99 398.82 30.54 135 62 162 100 376.14 28.59 136 63 160 97 414.60 27.16 137 63 161 98 414.38 27.18 138 63 219 156 269.21 27.73 139 63 221 158 279.27 19.16 140 63 238 175 230.97 0.14 141 64 153 89 438.00 28.07 142 64 154 90 438.00 28.07 143 64 155 91 438.03 28.07 144 64 156 92 438.00 28.07 145 64 157 93 437.95 28.07 146 64 159 95 415.65 26.92 147 64 160 96 415.50 26.91 148 64 209 145 271.77 26.91 149 64 219 155 272.36 26.34 150 64 221 157 283.30 20.58 151 65 68 3 815.64 0.11 152 65 69 4 731.34 22.44 153 65 141 76 442.17 30.77 154 65 154 89 438.00 28.06 155 65 159 94 435.25 29.42 156 65 160 95 417.60 26.61 157 65 161 96 416.97 26.30 158 65 221 156 285.94 19.39 159 66 69 3 835.66 0.87 160 66 154 88 438.05 28.07 161 66 207 141 286.86 19.33 162 66 208 142 283.32 21.19 163 66 209 143 278.00 23.86 164 67 70 3 840.34 3.21 165 67 115 48 532.39 20.23 166 67 205 138 289.22 18.15 167 67 206 139 289.10 18.24 168 67 207 140 286.89 19.35 169 68 71 3 855.55 2.09 170 68 72 4 840.34 3.21 171 68 166 98 423.78 25.34 172 68 205 137 289.23 18.17 173 68 206 138 289.19 18.27 174 68 207 139 287.03 19.41 175 68 221 153 289.03 18.41 176 68 223 155 289.22 18.15 177 69 72 3 855.55 2.09 178 69 73 4 814.94 0.62 179 69 132 63 500.19 31.44 180 69 136 67 462.51 22.74 181 69 141 72 461.88 22.73 182 69 153 84 451.75 23.98 183 69 154 85 450.38 24.29 184 69 159 90 449.64 23.93 185 70 73 3 818.17 0.99 186 70 74 4 814.85 0.66 187 70 112 42 533.22 20.09 188 70 115 45 533.21 20.09 189 70 116 46 533.21 20.09 190 70 120 50 533.05 20.12 191 70 205 135 289.60 18.34 192 71 74 3 829.35 4.02 193 71 75 4 814.83 0.67 194 71 169 98 419.35 27.01 195 71 170 99 419.23 27.07 196 71 173 102 412.40 27.56 197 71 188 117 363.62 27.52 198 71 192 121 357.88 26.05 199 71 205 134 290.49 18.78 200 71 223 152 290.85 18.93 201 72 75 3 817.57 0.78 202 72 76 4 814.75 0.63 203 72 136 64 462.53 22.75 204 72 139 67 462.28 22.77 205 72 141 69 462.05 22.82 206 72 169 97 419.35 27.01 207 73 76 3 818.08 0.40 208 73 77 4 808.62 2.43 209 73 120 47 533.21 20.09 210 73 123 50 533.13 20.11 211 74 77 3 815.36 0.35 212 74 78 4 813.98 0.58 213 74 83 9 550.77 23.94 214 74 108 34 533.50 20.11 215 74 112 38 533.45 20.09 216 74 120 46 533.21 20.09 217 74 188 114 363.77 27.45 218 75 78 3 832.73 6.86 219 75 79 4 750.77 31.03 220 75 139 64 462.47 22.78 221 75 169 94 419.70 26.83 222 75 173 98 412.41 27.57 223 75 178 103 371.62 25.84 224 75 180 105 371.34 25.88 225 75 188 113 363.88 27.49 226 76 79 3 813.99 0.58 227 76 80 4 813.58 0.38 228 76 127 51 532.78 20.23 229 76 132 56 530.90 19.84 230 76 135 59 466.16 24.56 231 76 136 60 507.91 27.54 232 76 139 63 462.51 22.77 233 77 80 3 834.07 0.18 234 77 81 4 813.59 0.38 235 77 82 5 643.11 9.71 236 77 83 6 563.73 30.41 237 77 123 46 533.16 20.11 238 77 127 50 532.81 20.25 239 78 81 3 804.84 2.38 240 78 83 5 576.41 36.76 241 78 85 7 546.39 26.12 242 78 108 30 533.95 20.14 243 78 184 106 367.73 27.45 244 78 187 109 365.10 27.50 245 78 188 110 365.05 27.50 246 79 135 56 531.22 19.95 247 79 180 101 371.34 25.88 248 79 181 102 371.34 25.88 249 79 184 105 367.95 27.55 250 80 127 47 532.91 20.20 251 80 131 51 532.66 20.18 252 80 135 55 532.23 20.13 253 82 123 41 533.36 20.11 254 82 126 44 533.13 20.12 255 82 127 45 532.91 20.20 256 82 131 49 532.86 20.23 257 83 88 5 566.52 29.58 258 83 90 7 534.62 20.29 259 83 108 25 534.10 20.18 260 83 123 40 533.39 20.12 261 84 88 4 634.46 7.03 262 85 88 3 823.78 4.80 263 86 187 101 365.03 27.50 264 88 107 19 538.54 19.28 265 89 105 16 538.81 19.27 266 89 106 17 538.70 19.29 267 90 105 15 538.86 19.29 268 90 106 16 538.75 19.28 269 90 107 17 538.75 19.28 270 90 108 18 538.72 19.30 271 90 111 21 538.66 19.32 272 90 119 29 534.08 20.13 273 90 123 33 534.03 20.15 274 91 105 14 538.87 19.29 275 92 95 3 920.06 2.50 276 92 97 5 788.40 5.75 277 92 103 11 538.90 19.27 278 92 104 12 538.90 19.28 279 92 105 13 538.90 19.28 280 96 103 7 580.34 16.75 281 97 102 5 719.88 23.84 282 97 103 6 606.10 29.63 283 97 118 21 541.00 20.29 284 97 119 22 541.00 20.29 285 98 102 4 831.67 2.83 286 99 102 3 840.03 3.14 287 99 103 4 826.00 0.01 288 100 103 3 826.00 0.01 289 101 104 3 901.53 0.90 290 101 114 13 544.02 21.05 291 102 106 4 834.27 7.31 292 102 114 12 544.01 21.05 293 102 115 13 544.00 21.05 294 102 116 14 543.90 21.05 295 102 117 15 543.96 21.07 296 102 118 16 543.91 21.06 297 106 110 4 728.23 33.09 298 106 111 5 692.35 15.15 299 106 114 8 674.74 23.21 300 106 116 10 579.98 28.44 301 106 119 13 548.91 20.99 302 107 110 3 801.91 6.40 303 107 111 4 791.80 1.30 304 108 111 3 819.21 3.48 305 108 112 4 819.22 3.47 306 108 187 79 364.89 27.53 307 108 188 80 364.07 27.46 308 108 191 83 361.46 27.12 309 109 112 3 822.12 2.02 310 109 113 4 819.22 3.47 311 109 191 82 361.04 26.93 312 109 193 84 361.04 26.93 313 109 194 85 361.01 26.94 314 110 113 3 819.65 3.25 315 110 114 4 819.22 3.47 316 111 114 3 821.69 3.76 317 111 115 4 739.20 27.57 318 111 116 5 677.33 35.68 319 111 119 8 612.31 30.02 320 111 120 9 612.32 30.02 321 112 115 3 735.47 23.64 322 112 120 8 635.28 18.55 323 112 191 79 361.04 26.92 324 112 192 80 360.90 26.95 325 112 205 93 290.52 18.77 326 113 194 81 357.05 25.66 327 115 205 90 289.62 18.32 328 116 119 3 823.66 1.30 329 116 120 4 818.82 0.66 330 117 120 3 823.65 1.31 331 117 121 4 818.31 0.92 332 117 155 38 456.10 23.62 333 118 121 3 818.82 0.67 334 118 122 4 818.30 0.92 335 119 122 3 819.07 0.75 336 119 123 4 818.30 0.92 337 120 123 3 817.11 1.51 338 120 124 4 808.72 2.17 339 120 154 34 458.21 23.06 340 121 124 3 821.64 2.23 341 121 125 4 815.46 0.60 342 121 151 30 458.09 22.89 343 121 154 33 458.23 23.07 344 122 125 3 825.87 4.34 345 122 126 4 815.47 0.60 346 123 126 3 828.36 7.04 347 123 127 4 815.46 0.60 348 124 127 3 819.48 0.93 349 124 128 4 814.76 0.94 350 124 132 8 691.24 23.32 351 124 150 26 458.73 23.10 352 124 151 27 458.68 23.08 353 124 154 30 458.62 23.11 354 125 128 3 827.20 6.63 355 125 150 25 458.75 23.11 356 125 151 26 458.74 23.11 357 126 131 5 828.26 1.47 358 127 131 4 815.26 0.77 359 127 132 5 807.81 4.48 360 128 131 3 841.57 2.96 361 128 132 4 815.26 0.77 362 128 150 22 458.86 23.14 363 129 132 3 837.77 3.29 364 129 133 4 815.30 0.73 365 129 146 17 461.79 22.71 366 129 150 21 458.86 23.14 367 130 133 3 824.52 3.34 368 130 134 4 818.17 0.70 369 131 134 3 825.55 2.98 370 131 135 4 809.46 5.06 371 132 135 3 829.49 1.02 372 132 136 4 809.31 5.13 373 132 146 14 461.82 22.71 374 132 150 18 458.85 23.14 375 132 153 21 458.78 23.18 376 133 136 3 830.82 1.61 377 133 137 4 814.45 2.56 378 133 144 11 461.95 22.70 379 133 146 13 461.83 22.71 380 134 137 3 827.08 0.27 381 134 138 4 806.12 3.56 382 135 138 3 809.22 2.01 383 135 139 4 798.50 0.79 384 135 179 44 371.48 25.82 385 136 139 3 806.05 4.76 386 136 140 4 798.29 0.89 387 136 141 5 703.23 36.70 388 136 144 8 461.88 22.73 389 136 146 10 461.87 22.73 390 136 153 17 458.67 23.12 391 136 159 23 450.63 23.96 392 137 140 3 807.76 3.85 393 137 144 7 508.63 45.95 394 138 179 41 371.49 25.82 395 139 169 30 419.74 26.82 396 139 178 39 371.55 25.83 397 139 179 40 371.51 25.81 398 140 144 4 803.86 1.56 399 140 165 25 437.39 27.68 400 141 144 3 827.78 1.33 401 141 159 18 450.59 23.96 402 141 161 20 443.66 26.61 403 141 165 24 437.38 27.68 404 141 169 28 419.75 26.80 405 142 160 18 449.72 23.91 406 142 161 19 442.73 26.14 407 142 165 23 437.38 27.68 408 143 158 15 451.13 23.96 409 143 159 16 450.63 23.96 410 143 160 17 450.13 23.86 411 144 159 15 450.75 23.90 412 145 158 13 451.36 24.18 413 145 159 14 450.75 23.89 414 146 150 4 805.27 0.04 415 146 153 7 496.80 42.83 416 147 153 6 623.10 5.78 417 147 157 10 452.72 24.27 418 147 158 11 452.39 24.30 419 148 152 4 829.24 3.82 420 148 157 9 452.73 24.27 421 149 152 3 839.67 1.37 422 149 153 4 812.31 0.82 423 150 153 3 852.68 0.21 424 150 154 4 863.41 0.07 425 151 154 3 971.26 1.66 426 151 155 4 852.24 1.08 427 152 155 3 850.07 2.17 428 152 156 4 817.45 0.01 429 152 157 5 647.61 27.52 430 153 157 4 505.19 50.77 431 153 159 6 450.76 23.88 432 154 159 5 491.88 44.61 433 161 165 4 808.24 3.81 434 161 166 5 808.23 3.81 435 161 221 60 290.14 18.96 436 162 165 3 819.33 1.74 437 162 166 4 808.25 3.81 438 162 221 59 290.51 18.96 439 163 166 3 822.98 3.56 440 163 167 4 808.25 3.81 441 163 221 58 290.53 18.97 442 163 236 73 248.39 2.11 443 163 238 75 245.85 1.18 444 164 167 3 818.94 0.50 445 164 168 4 808.22 3.81 446 165 168 3 817.57 0.84 447 165 169 4 806.24 2.85 448 166 169 3 817.57 0.84 449 166 170 4 810.98 8.02 450 166 221 55 290.73 18.96 451 166 223 57 289.46 18.23 452 166 236 70 248.44 2.14 453 167 170 3 817.68 4.67 454 167 171 4 823.10 1.96 455 167 234 67 248.88 2.14 456 167 235 68 248.87 2.14 457 167 236 69 248.55 2.15 458 168 171 3 823.10 1.96 459 168 172 4 823.10 1.96 460 169 172 3 825.80 0.61 461 169 173 4 814.70 6.16 462 169 178 9 558.09 18.98 463 170 173 3 825.80 0.61 464 170 174 4 801.64 5.80 465 170 223 53 290.94 18.88 466 170 224 54 290.02 18.41 467 170 225 55 289.57 18.17 468 170 235 65 248.93 2.14 469 171 174 3 807.55 2.85 470 171 175 4 795.52 2.73 471 171 234 63 249.30 2.09 472 172 175 3 806.90 0.69 473 172 176 4 791.72 4.63 474 172 177 5 661.15 13.74 475 172 178 6 563.50 21.68 476 173 176 3 828.65 3.98 477 173 178 5 640.25 26.55 478 173 185 12 365.27 27.90 479 173 188 15 363.97 27.48 480 173 189 16 363.62 27.51 481 173 192 19 362.77 27.70 482 173 225 52 292.35 18.18 483 174 189 15 363.64 27.53 484 174 225 51 292.37 18.18 485 174 227 53 291.39 17.59 486 174 232 58 250.80 2.69 487 174 234 60 250.70 2.72 488 176 182 6 371.16 26.00 489 176 185 9 369.79 26.64 490 176 189 13 365.55 28.26 491 177 185 8 506.03 43.48 492 178 185 7 626.21 6.08 493 180 185 5 795.95 6.13 494 181 184 3 815.52 4.58 495 181 185 4 807.30 0.47 496 182 185 3 807.31 0.47 497 182 186 4 804.62 1.80 498 183 186 3 821.24 7.38 499 183 187 4 804.62 1.80 500 184 187 3 818.51 8.73 501 184 188 4 807.19 0.53 502 185 188 3 821.20 7.40 503 185 189 4 807.35 0.45 504 186 189 3 815.09 3.14 505 186 190 4 807.25 0.40 506 187 190 3 821.98 0.29 507 187 191 4 807.24 0.40 508 188 191 3 807.93 0.12 509 188 192 4 807.07 0.31 510 189 192 3 823.38 5.50 511 189 193 4 808.10 0.21 512 189 203 14 292.79 18.66 513 189 225 36 292.48 18.18 514 190 193 3 826.49 0.78 515 192 203 11 292.74 18.68 516 192 204 12 292.06 18.41 517 192 205 13 291.18 18.91 518 192 225 33 292.43 18.23 519 193 201 8 293.40 18.63 520 193 202 9 293.28 18.65 521 193 203 10 292.93 18.73 522 194 202 8 293.35 18.66 523 194 204 10 292.94 18.73 524 195 200 5 580.74 79.52 525 195 201 6 293.51 18.61 526 195 202 7 293.40 18.63 527 196 200 4 784.76 8.75 528 196 201 5 293.49 18.62 529 197 200 3 804.99 1.35 530 199 228 29 550.89 110.94 531 199 229 30 550.90 110.95 532 200 226 26 550.94 110.92 533 200 227 27 550.89 110.94 534 200 228 28 550.88 110.94 535 200 263 63 37.38 0.99 536 200 264 64 33.04 0.31 537 200 265 65 32.84 0.25 538 201 226 25 550.95 110.92 539 201 227 26 550.90 110.95 540 202 225 23 551.04 110.89 541 202 226 24 550.95 110.92 542 202 264 62 32.81 0.24 543 203 224 21 551.07 110.89 544 203 225 22 551.06 110.89 545 203 227 24 551.09 110.92 546 204 222 18 551.18 110.90 547 204 223 19 551.15 110.89 548 204 224 20 551.07 110.89 549 204 235 31 508.99 131.94 550 205 222 17 551.19 110.90 551 205 223 18 551.16 110.89 552 206 221 15 551.26 110.88 553 206 222 16 551.19 110.90 554 206 250 44 72.06 0.84 555 206 251 45 71.98 0.89 556 207 220 13 551.38 110.92 557 207 221 14 551.28 110.88 558 207 250 43 72.09 0.85 559 208 211 3 1027.65 0.17 560 208 219 11 551.48 110.89 561 208 220 12 551.38 110.92 562 208 241 33 508.12 132.31 563 208 244 36 435.50 116.67 564 208 247 39 72.34 0.82 565 209 218 9 551.56 110.89 566 209 219 10 551.53 110.92 567 210 218 8 551.81 110.80 568 211 217 6 552.35 110.59 569 211 218 7 551.88 110.77 570 211 241 30 508.28 132.31 571 211 242 31 508.05 132.27 572 211 243 32 507.95 132.22 573 211 244 33 507.37 131.94 574 212 216 4 718.84 27.48 575 212 217 5 552.44 110.64 576 212 218 6 552.23 110.64 577 213 216 3 757.20 9.03 578 213 217 4 552.49 110.64 579 213 242 29 508.52 132.19 580 214 242 28 508.53 132.19 581 217 240 23 508.55 132.19 582 217 241 24 508.48 132.18 583 217 242 25 508.25 132.27 584 218 240 22 508.53 132.18 585 218 241 23 508.41 132.19 586 219 238 19 508.55 132.19 587 219 239 20 508.54 132.18 588 219 241 22 508.14 132.32 589 220 238 18 508.53 132.19 590 220 239 19 508.48 132.21 591 220 241 21 508.12 132.31 592 220 247 27 72.20 0.85 593 220 250 30 72.06 0.83 594 221 237 16 508.53 132.19 595 221 238 17 508.38 132.22 596 221 250 29 72.05 0.84 597 222 235 13 509.10 132.25 598 222 236 14 508.68 132.11 599 222 237 15 508.53 132.18 600 222 250 28 72.01 0.83 601 222 251 29 71.75 0.83 602 222 254 32 56.88 0.41 603 222 255 33 61.73 0.10 604 222 258 36 53.16 1.28 605 223 235 12 509.09 132.25 606 223 236 13 508.65 132.12 607 224 234 10 509.58 132.22 608 224 235 11 509.24 132.30 609 225 232 7 509.78 132.21 610 225 233 8 509.66 132.20 611 225 234 9 509.58 132.22 612 225 235 10 509.57 132.22 613 226 232 6 509.80 132.21 614 226 233 7 509.74 132.22 615 226 235 9 509.69 132.19 616 226 258 32 45.73 0.08 617 226 263 37 32.81 0.25 618 226 264 38 32.80 0.24 619 227 231 4 694.46 39.90 620 227 232 5 509.82 132.20 621 228 231 3 804.90 0.88 622 228 233 5 694.46 39.90 623 228 262 34 40.98 0.03 624 228 263 35 32.73 0.21 625 233 263 30 32.69 0.19 626 235 258 23 45.73 0.07 627 237 250 13 71.95 0.80 628 237 253 16 61.20 0.15 629 237 254 17 56.85 0.40 630 239 246 7 453.09 60.06 631 239 249 10 72.11 0.82 632 239 250 11 72.03 0.82 633 241 244 3 912.35 3.81 634 241 246 5 757.57 15.30 635 241 247 6 348.75 139.02 636 243 246 3 849.58 2.64 637 244 247 3 838.66 8.10 638 245 248 3 895.52 1.20 639 246 249 3 838.07 0.57 640 247 250 3 831.50 2.71 641 248 251 3 832.59 1.47 642 248 252 4 798.81 4.15 643 249 252 3 810.18 1.54 644 249 253 4 789.24 0.70 645 249 254 5 798.78 4.08 646 250 254 4 807.35 0.14 647 251 254 3 824.56 4.58 648 251 255 4 798.79 4.08 649 252 255 3 814.05 0.68 650 252 256 4 799.51 3.72 651 253 256 3 819.12 1.85 652 253 257 4 807.93 0.32 653 254 257 3 810.25 1.48 654 254 258 4 807.93 0.32 655 255 258 3 814.35 3.52 656 255 259 4 807.28 0.00 657 255 264 9 335.42 151.17 658 255 266 11 25.75 2.44 659 256 259 3 823.77 0.55 660 256 260 4 810.26 1.47 661 257 260 3 826.39 1.85 662 257 261 4 815.00 3.84 663 258 261 3 819.49 2.17 664 258 262 4 803.47 4.87 665 258 263 5 651.65 38.92 666 258 264 6 611.18 29.07 667 259 262 3 803.48 4.87 668 259 264 5 362.78 164.85