BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 17 63 43 59.54 0.52 2 17 64 44 60.48 0.07 3 18 61 40 70.97 0.53 4 18 62 41 73.42 0.70 5 18 63 42 67.07 0.58 6 18 64 43 79.05 0.58 7 18 96 75 74.04 1.72 8 18 97 76 74.01 1.71 9 18 246 216 74.00 1.74 10 19 64 42 79.36 0.67 11 19 66 44 79.75 0.52 12 19 94 72 79.68 0.54 13 19 95 73 79.17 0.58 14 19 96 74 78.05 0.07 15 19 97 75 77.88 0.00 16 20 63 40 79.68 0.53 17 20 64 41 79.70 0.54 18 20 65 42 79.72 0.53 19 20 66 43 79.75 0.52 20 20 97 74 78.05 0.07 21 20 99 76 79.09 0.58 22 21 66 42 81.12 0.09 23 21 68 44 81.36 0.14 24 21 86 62 79.67 0.53 25 21 89 65 80.57 0.15 26 21 95 71 79.74 0.54 27 21 97 73 79.67 0.53 28 21 98 74 79.48 0.43 29 21 99 75 79.67 0.53 30 22 29 7 195.24 1.16 31 22 66 41 81.43 0.18 32 22 67 42 81.44 0.17 33 22 68 43 81.45 0.16 34 22 86 61 79.83 0.53 35 22 99 74 79.67 0.53 36 22 100 75 79.67 0.53 37 23 29 6 358.50 80.03 38 23 67 41 81.47 0.17 39 23 68 42 81.48 0.17 40 23 71 45 81.38 0.13 41 23 72 46 81.37 0.13 42 23 73 47 81.14 0.03 43 23 86 60 81.45 0.15 44 23 100 74 79.73 0.53 45 24 29 5 358.51 80.03 46 24 30 6 355.75 81.41 47 24 31 7 355.75 81.41 48 24 44 17 81.44 0.17 49 24 67 40 81.84 0.25 50 25 28 3 691.46 0.33 51 25 29 4 607.60 6.21 52 25 73 45 81.65 0.18 53 25 101 73 81.12 0.20 54 25 190 153 71.28 0.38 55 25 212 175 71.27 0.35 56 26 30 4 626.61 1.95 57 26 215 177 71.22 0.35 58 27 30 3 693.01 2.53 59 27 46 16 81.45 0.18 60 27 47 17 81.45 0.17 61 27 215 176 71.22 0.35 62 27 270 231 71.25 0.33 63 27 271 232 71.25 0.33 64 28 46 15 81.66 0.27 65 28 47 16 81.45 0.17 66 28 48 17 81.47 0.17 67 28 49 18 81.78 0.24 68 28 101 70 81.67 0.20 69 28 119 88 81.42 0.16 70 28 211 171 71.35 0.36 71 28 212 172 71.32 0.38 72 28 236 196 81.70 0.21 73 28 271 231 71.25 0.33 74 29 44 12 81.90 0.27 75 29 45 13 81.90 0.27 76 29 46 14 81.86 0.26 77 29 48 16 81.80 0.24 78 29 49 17 81.83 0.26 79 29 67 35 82.06 0.28 80 30 44 11 81.94 0.26 81 30 45 12 81.92 0.27 82 30 46 13 81.90 0.27 83 30 270 228 71.26 0.33 84 31 43 9 82.03 0.28 85 31 44 10 81.95 0.27 86 32 38 3 578.46 15.81 87 32 42 7 82.12 0.25 88 32 43 8 82.06 0.28 89 32 44 9 82.04 0.27 90 32 45 10 81.98 0.26 91 38 42 4 639.88 4.32 92 38 43 5 432.40 81.70 93 39 42 3 693.02 2.75 94 39 43 4 641.16 12.58 95 40 43 3 693.00 2.76 96 40 44 4 687.58 5.47 97 41 44 3 684.32 1.17 98 41 63 22 118.47 0.87 99 41 64 23 117.12 0.20 100 41 65 24 108.30 0.62 101 44 49 5 618.02 0.34 102 44 65 21 121.20 0.55 103 44 67 23 121.30 0.50 104 45 49 4 619.76 0.54 105 45 270 216 108.01 0.64 106 46 49 3 652.07 1.67 107 46 50 4 634.43 0.33 108 46 270 215 107.82 0.55 109 47 50 3 670.93 2.60 110 47 51 4 634.44 0.33 111 47 118 71 119.91 0.18 112 47 119 72 113.33 0.60 113 47 263 207 110.31 0.92 114 47 266 210 108.14 0.68 115 47 267 211 108.11 0.69 116 47 270 214 107.83 0.54 117 47 271 215 108.02 0.64 118 48 51 3 637.57 1.04 119 48 52 4 635.29 0.10 120 48 99 51 121.16 0.51 121 48 100 52 121.11 0.54 122 48 101 53 121.10 0.54 123 48 118 70 120.84 0.49 124 49 52 3 643.51 1.93 125 49 53 4 641.22 3.07 126 49 65 16 123.91 0.61 127 50 53 3 662.19 4.29 128 50 54 4 641.38 3.16 129 50 266 207 108.23 0.72 130 50 269 210 108.14 0.68 131 50 270 211 108.02 0.64 132 51 54 3 658.89 5.59 133 51 55 4 647.93 0.12 134 51 99 48 122.94 1.03 135 51 118 67 121.32 0.47 136 51 238 178 122.91 1.04 137 51 257 197 121.59 0.40 138 51 263 203 120.95 0.64 139 51 266 206 114.74 0.88 140 52 55 3 657.61 4.83 141 52 56 4 657.50 4.90 142 52 63 11 184.80 0.37 143 52 65 13 123.94 0.62 144 52 99 47 122.94 1.03 145 53 56 3 657.65 4.86 146 53 57 4 657.54 4.92 147 53 63 10 354.15 84.33 148 54 57 3 652.11 7.65 149 54 58 4 657.66 4.88 150 54 257 194 122.84 0.99 151 54 262 199 122.67 0.95 152 55 58 3 657.68 4.88 153 55 59 4 657.66 4.88 154 55 61 6 613.31 1.21 155 55 97 42 124.03 0.64 156 55 99 44 123.91 0.62 157 55 238 174 123.47 0.77 158 55 250 186 123.84 0.58 159 55 253 189 122.92 1.03 160 55 257 193 122.85 0.99 161 56 59 3 669.95 1.25 162 56 60 4 641.59 12.93 163 56 61 5 604.50 0.97 164 56 63 7 539.70 8.44 165 57 60 3 652.11 7.67 166 58 253 186 123.88 0.60 167 58 256 189 123.86 0.60 168 59 249 181 123.98 0.62 169 59 250 182 123.95 0.61 170 59 253 185 123.94 0.60 171 68 71 3 634.94 5.70 172 68 72 4 627.10 9.04 173 68 85 17 174.77 1.66 174 68 86 18 172.69 0.96 175 68 89 21 174.19 1.70 176 70 84 14 177.22 1.20 177 70 85 15 177.19 1.18 178 72 84 12 177.30 1.18 179 72 85 13 177.27 1.17 180 72 86 14 177.27 1.18 181 73 85 12 177.28 1.17 182 73 86 13 177.28 1.17 183 73 87 14 177.27 1.17 184 73 101 28 176.49 1.01 185 73 102 29 176.31 1.00 186 73 108 35 177.04 1.10 187 74 83 9 177.99 1.23 188 74 84 10 177.67 1.34 189 74 85 11 177.28 1.17 190 74 87 13 177.22 1.15 191 74 108 34 176.97 1.06 192 75 83 8 178.01 1.22 193 75 84 9 178.00 1.22 194 76 81 5 186.22 2.69 195 76 82 6 178.38 1.22 196 76 83 7 178.10 1.26 197 77 80 3 684.78 3.16 198 77 81 4 340.71 79.92 199 77 82 5 340.70 79.92 200 78 81 3 637.84 4.20 201 82 92 10 388.03 65.06 202 83 87 4 632.87 7.03 203 83 88 5 604.60 7.08 204 83 91 8 386.56 66.98 205 83 92 9 388.05 65.06 206 83 107 24 252.40 0.31 207 83 108 25 251.77 0.15 208 83 111 28 252.41 0.27 209 84 87 3 693.47 1.20 210 84 88 4 621.50 1.45 211 85 88 3 669.54 1.30 212 85 89 4 642.83 6.60 213 86 89 3 659.28 6.44 214 86 90 4 648.29 11.93 215 86 98 12 252.42 0.34 216 86 100 14 252.43 0.33 217 86 102 16 248.94 1.97 218 87 90 3 674.46 1.16 219 87 91 4 628.75 2.16 220 87 102 15 249.05 2.02 221 87 108 21 259.51 0.68 222 87 111 24 260.34 1.02 223 87 112 25 259.52 0.68 224 88 91 3 648.28 11.93 225 88 92 4 622.27 5.40 226 89 93 4 613.25 2.07 227 89 94 5 391.62 63.14 228 89 95 6 382.82 58.75 229 90 93 3 632.31 1.00 230 90 95 5 382.24 58.47 231 90 96 6 382.24 58.47 232 90 97 7 261.88 1.70 233 90 98 8 261.87 1.70 234 90 112 22 261.86 1.71 235 90 239 140 261.87 1.71 236 90 240 141 261.88 1.71 237 90 241 142 261.88 1.71 238 91 111 20 261.83 1.72 239 91 112 21 261.85 1.72 240 95 241 137 315.19 2.44 241 96 240 135 315.13 2.42 242 96 241 136 315.19 2.44 243 96 246 141 315.22 2.46 244 97 238 132 315.07 2.42 245 97 239 133 315.12 2.42 246 97 240 134 315.17 2.44 247 97 250 144 315.17 2.44 248 98 102 4 670.76 4.08 249 98 238 131 315.13 2.43 250 98 239 132 315.17 2.44 251 99 238 130 315.17 2.44 252 100 118 18 315.16 2.44 253 101 212 102 310.80 3.25 254 101 236 126 315.19 2.46 255 102 108 6 624.46 3.98 256 102 112 10 421.51 50.50 257 102 114 12 315.64 2.37 258 102 236 125 315.31 2.42 259 102 237 126 315.34 2.42 260 102 239 128 315.66 2.37 261 103 108 5 625.04 3.69 262 103 114 11 315.69 2.37 263 103 158 46 315.34 2.41 264 103 209 97 315.23 2.39 265 103 234 122 315.34 2.42 266 103 235 123 315.34 2.42 267 103 236 124 315.34 2.42 268 104 109 5 628.43 1.99 269 104 114 10 315.69 2.37 270 104 115 11 315.69 2.37 271 104 117 13 315.53 2.35 272 104 235 122 315.36 2.42 273 104 236 123 315.39 2.41 274 104 237 124 315.54 2.35 275 105 108 3 658.41 2.12 276 105 109 4 633.00 0.28 277 105 158 44 315.48 2.33 278 106 109 3 653.00 4.75 279 106 110 4 638.53 2.50 280 107 110 3 667.76 6.79 281 108 111 3 674.99 1.23 282 108 112 4 633.00 0.27 283 108 114 6 605.54 6.74 284 109 112 3 652.50 10.02 285 109 114 5 605.04 6.99 286 111 242 122 424.75 0.72 287 112 239 118 422.78 1.12 288 112 240 119 423.92 0.60 289 112 241 120 423.97 0.61 290 112 242 121 424.75 0.71 291 113 239 117 423.92 0.60 292 113 240 118 424.71 0.70 293 113 241 119 424.84 0.74 294 113 242 120 425.03 0.64 295 113 243 121 425.05 0.65 296 114 237 114 414.89 1.18 297 114 238 115 423.92 0.61 298 114 239 116 424.68 0.73 299 115 237 113 423.88 0.61 300 115 238 114 424.75 0.71 301 115 250 126 424.66 0.66 302 115 251 127 423.43 0.86 303 115 254 130 423.32 0.91 304 116 251 126 424.88 0.69 305 116 254 129 424.68 0.70 306 116 255 130 424.43 0.84 307 117 235 109 422.84 1.13 308 117 236 110 431.90 1.18 309 117 237 111 441.63 1.82 310 117 254 128 424.90 0.70 311 118 236 109 449.91 4.11 312 118 237 110 449.92 4.11 313 118 238 111 449.94 4.11 314 118 254 127 424.94 0.68 315 118 263 136 421.44 1.12 316 119 211 83 392.34 1.26 317 119 235 107 449.91 4.11 318 119 236 108 449.92 4.11 319 119 267 139 407.76 0.89 320 119 271 143 388.55 0.08 321 120 211 82 406.22 0.67 322 120 234 105 449.92 4.11 323 120 235 106 449.92 4.11 324 121 209 79 441.57 8.28 325 121 210 80 411.17 2.56 326 121 211 81 406.80 0.38 327 121 233 103 449.92 4.11 328 121 234 104 449.92 4.11 329 121 235 105 449.94 4.11 330 121 236 106 449.99 4.11 331 122 209 78 448.93 4.63 332 122 210 79 448.85 4.65 333 122 230 99 438.41 0.68 334 122 232 101 449.89 4.11 335 123 159 27 449.31 4.50 336 123 162 30 450.00 4.18 337 123 163 31 450.05 4.15 338 123 207 75 449.81 4.22 339 123 208 76 449.06 4.59 340 123 209 77 448.98 4.61 341 123 230 98 449.10 4.40 342 123 231 99 449.89 4.11 343 123 232 100 449.94 4.10 344 123 234 102 450.03 4.11 345 124 207 74 449.98 4.15 346 124 208 75 449.91 4.17 347 124 210 77 449.25 4.50 348 124 213 80 449.00 4.57 349 124 226 93 436.78 0.44 350 124 230 97 449.80 4.12 351 124 231 98 449.94 4.11 352 125 139 5 450.18 4.08 353 125 140 6 450.15 4.09 354 125 143 9 450.15 4.09 355 125 205 71 450.04 4.11 356 125 206 72 450.04 4.12 357 125 207 73 450.00 4.14 358 125 213 79 449.25 4.48 359 125 226 92 438.47 0.59 360 125 227 93 440.96 0.59 361 125 231 97 449.98 4.11 362 126 194 59 450.03 4.12 363 126 197 62 450.06 4.11 364 126 205 70 450.06 4.11 365 126 206 71 450.05 4.12 366 126 208 73 450.06 4.12 367 126 213 78 449.43 4.39 368 126 216 81 449.16 4.44 369 126 224 89 436.76 0.43 370 126 225 90 438.81 0.44 371 126 226 91 440.94 0.62 372 127 197 61 450.08 4.09 373 127 205 69 450.09 4.09 374 127 224 88 438.25 0.59 375 127 225 89 440.98 0.65 376 127 227 91 449.63 4.20 377 128 197 60 450.10 4.09 378 128 198 61 450.09 4.09 379 128 201 64 450.10 4.10 380 128 202 65 450.10 4.10 381 128 223 86 438.13 0.54 382 128 224 87 438.30 0.61 383 128 225 88 448.39 4.14 384 138 142 4 636.91 3.94 385 138 228 90 448.95 4.59 386 139 142 3 637.10 4.03 387 139 143 4 654.35 4.78 388 140 143 3 654.75 4.98 389 140 144 4 654.34 4.77 390 140 170 30 453.45 5.41 391 140 171 31 453.43 5.40 392 140 174 34 453.43 5.40 393 140 231 91 449.67 4.26 394 141 144 3 654.36 4.78 395 141 145 4 654.34 4.77 396 141 170 29 453.44 5.40 397 142 145 3 670.29 0.51 398 142 146 4 636.18 4.32 399 143 146 3 665.83 2.74 400 143 149 6 533.11 6.71 401 143 151 8 453.30 5.40 402 143 174 31 453.46 5.40 403 143 205 62 450.81 4.16 404 143 207 64 450.77 4.19 405 144 151 7 453.43 5.40 406 144 170 26 454.70 6.00 407 144 173 29 453.52 5.39 408 144 174 30 453.45 5.41 409 145 173 28 461.00 9.11 410 149 202 53 489.10 14.71 411 149 203 54 488.97 14.64 412 149 204 55 488.96 14.65 413 149 205 56 488.95 14.65 414 150 177 27 510.88 10.74 415 150 185 35 510.49 10.65 416 150 203 53 489.10 14.71 417 150 204 54 489.06 14.71 418 150 205 55 489.06 14.71 419 151 174 23 510.91 10.73 420 151 177 26 510.90 10.73 421 151 178 27 510.86 10.73 422 151 185 34 510.56 10.66 423 151 204 53 489.05 14.74 424 151 205 54 488.86 14.80 425 151 207 56 488.75 14.76 426 152 178 26 510.88 10.75 427 152 185 33 510.56 10.67 428 152 204 52 489.13 14.73 429 152 205 53 488.92 14.81 430 152 206 54 488.85 14.81 431 152 207 55 488.65 14.91 432 153 159 6 513.08 9.76 433 153 160 7 513.00 9.77 434 153 163 10 512.99 9.76 435 153 178 25 510.92 10.73 436 153 186 33 510.56 10.67 437 153 187 34 510.56 10.67 438 153 206 53 488.88 14.82 439 153 207 54 488.55 14.96 440 154 160 6 544.04 12.21 441 154 187 33 510.56 10.67 442 154 188 34 510.50 10.67 443 154 189 35 510.44 10.68 444 154 190 36 510.27 10.67 445 154 206 52 488.95 14.84 446 155 160 5 599.83 10.22 447 155 186 31 510.62 10.69 448 155 187 32 510.61 10.69 449 155 188 33 510.56 10.67 450 156 160 4 644.74 6.67 451 157 160 3 674.34 0.29 452 157 161 4 644.76 6.67 453 158 161 3 644.88 6.73 454 158 162 4 644.88 6.73 455 158 209 51 488.38 15.06 456 159 162 3 664.57 3.12 457 159 163 4 655.19 1.57 458 159 207 48 488.62 15.00 459 159 209 50 488.36 15.05 460 160 163 3 683.26 1.21 461 160 164 4 650.57 3.85 462 160 175 15 511.70 10.51 463 161 164 3 655.18 1.56 464 161 165 4 650.56 3.85 465 162 165 3 672.28 0.75 466 162 166 4 650.40 3.78 467 162 171 9 511.94 10.46 468 162 231 69 471.55 6.71 469 162 232 70 471.56 6.71 470 163 166 3 653.81 5.49 471 163 171 8 511.93 10.46 472 163 174 11 511.86 10.44 473 163 175 12 511.69 10.51 474 163 178 15 511.69 10.51 475 163 207 44 488.61 15.00 476 163 231 68 471.53 6.70 477 164 175 11 511.72 10.50 478 166 171 5 554.22 5.20 479 168 171 3 668.72 1.96 480 168 172 4 641.92 0.51 481 169 172 3 666.99 5.63 482 169 173 4 641.92 0.52 483 170 173 3 673.85 2.21 484 170 174 4 653.65 5.35 485 171 174 3 654.76 4.88 486 171 175 4 653.65 5.34 487 171 231 60 471.42 6.63 488 172 175 3 655.43 1.75 489 172 176 4 650.74 3.89 490 173 176 3 663.04 4.69 491 173 177 4 642.99 7.97 492 174 177 3 646.34 9.14 493 174 178 4 645.85 9.38 494 174 207 33 488.66 14.92 495 174 231 57 471.44 6.62 496 175 178 3 654.90 4.86 497 175 179 4 645.85 9.38 498 175 186 11 520.62 6.75 499 176 179 3 675.37 1.33 500 176 180 4 657.79 7.44 501 177 180 3 676.95 2.12 502 177 181 4 642.87 7.88 503 177 185 8 524.24 4.99 504 178 181 3 652.84 9.93 505 178 185 7 520.65 6.75 506 178 186 8 520.64 6.76 507 178 207 29 488.85 14.81 508 179 186 7 520.65 6.75 509 181 184 3 691.58 7.61 510 181 185 4 643.59 10.71 511 184 196 12 501.14 16.65 512 185 196 11 497.26 18.59 513 185 204 19 489.12 14.71 514 186 196 10 499.74 17.45 515 187 192 5 590.73 5.13 516 187 193 6 538.89 2.31 517 187 196 9 525.89 4.38 518 187 204 17 489.23 14.73 519 187 206 19 488.91 14.82 520 188 192 4 627.73 0.24 521 189 192 3 652.03 6.08 522 189 193 4 646.87 8.65 523 190 193 3 671.34 3.58 524 190 194 4 645.72 9.23 525 190 206 16 489.12 14.77 526 190 208 18 488.87 14.83 527 190 212 22 482.87 12.41 528 191 194 3 671.34 3.58 529 191 195 4 653.61 5.29 530 192 195 3 661.94 1.11 531 192 196 4 653.60 5.28 532 193 196 3 668.04 1.97 533 193 197 4 653.57 5.26 534 193 206 13 489.03 14.79 535 194 197 3 668.03 1.97 536 194 198 4 646.25 8.92 537 194 206 12 489.17 14.75 538 194 216 22 449.19 4.08 539 195 198 3 668.04 1.97 540 195 199 4 646.21 8.89 541 196 199 3 668.03 1.97 542 196 200 4 646.19 8.91 543 196 204 8 489.27 14.72 544 197 200 3 676.68 6.33 545 197 204 7 489.27 14.72 546 197 206 9 489.16 14.73 547 197 224 27 441.00 0.66 548 198 216 18 449.64 4.30 549 198 219 21 449.37 4.25 550 198 220 22 448.81 4.27 551 198 221 23 444.36 2.12 552 198 223 25 441.00 0.65 553 198 224 26 441.55 0.50 554 200 203 3 615.06 11.73 555 200 204 4 520.48 0.88 556 207 231 24 659.01 3.20 557 208 212 4 658.11 2.68 558 208 213 5 642.12 0.48 559 209 212 3 673.40 1.16 560 209 213 4 658.22 2.62 561 209 236 27 668.17 2.54 562 210 213 3 658.22 2.62 563 210 214 4 658.22 2.62 564 210 226 16 652.24 2.48 565 210 230 20 659.37 3.29 566 211 214 3 662.75 0.36 567 211 215 4 662.74 0.36 568 211 236 25 684.12 9.86 569 211 271 60 653.06 1.84 570 212 215 3 662.75 0.36 571 212 216 4 664.59 0.57 572 212 236 24 767.29 31.71 573 213 216 3 671.70 2.92 574 213 217 4 663.38 1.93 575 213 226 13 659.55 3.20 576 214 217 3 671.78 2.96 577 214 218 4 666.57 0.35 578 214 267 53 674.71 5.59 579 214 271 57 660.50 3.46 580 214 272 58 660.49 3.45 581 215 218 3 675.90 0.59 582 215 219 4 666.57 0.35 583 215 271 56 663.12 2.16 584 216 219 3 666.57 0.36 585 216 224 8 663.21 2.12 586 217 224 7 663.21 2.12 587 217 277 60 659.94 3.26 588 218 271 53 795.61 18.18 589 218 272 54 836.70 2.40 590 218 275 57 660.88 3.26 591 218 276 58 660.66 3.34 592 218 277 59 659.52 3.18 593 219 222 3 683.78 1.22 594 219 276 57 661.37 3.50 595 219 277 58 660.88 3.25 596 220 276 56 677.07 4.18 597 220 277 57 680.09 1.47