BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 17 15 35.51 2.08 2 2 20 18 31.18 3.58 3 2 21 19 23.50 0.12 4 2 24 22 23.48 0.14 5 2 49 47 28.61 2.33 6 2 50 48 23.48 0.13 7 2 51 49 35.98 1.84 8 3 17 14 36.02 1.83 9 3 51 48 35.98 1.84 10 4 9 5 798.00 6.32 11 4 13 9 45.52 2.12 12 4 14 10 36.07 1.85 13 4 17 13 36.06 1.85 14 4 51 47 36.23 1.93 15 4 52 48 36.23 1.93 16 4 53 49 56.59 0.39 17 4 129 125 62.16 0.41 18 4 132 128 62.01 0.48 19 5 9 4 806.77 1.93 20 5 53 48 57.60 0.12 21 6 9 3 816.24 2.04 22 6 53 47 61.90 0.54 23 6 54 48 62.20 0.42 24 6 55 49 62.23 0.42 25 6 129 123 62.20 0.42 26 6 132 126 62.20 0.42 27 6 133 127 62.20 0.42 28 6 136 130 62.20 0.42 29 7 55 48 62.59 0.60 30 9 13 4 840.94 4.34 31 9 129 120 81.78 0.03 32 9 175 166 89.75 0.08 33 10 13 3 846.72 1.46 34 10 14 4 846.72 1.46 35 10 129 119 90.84 0.39 36 10 175 165 90.88 0.41 37 11 14 3 859.42 4.88 38 11 15 4 846.73 1.47 39 11 172 161 85.11 0.24 40 11 173 162 89.57 0.70 41 11 175 164 92.61 0.37 42 12 15 3 847.43 1.13 43 12 16 4 844.87 2.40 44 13 16 3 853.55 1.95 45 13 17 4 840.94 4.30 46 14 17 3 845.68 1.92 47 14 18 4 836.34 6.58 48 14 129 115 97.92 1.66 49 14 157 143 92.86 0.29 50 14 161 147 92.84 0.27 51 14 168 154 92.86 0.29 52 14 171 157 96.31 0.95 53 14 172 158 95.44 0.62 54 15 18 3 845.63 1.89 55 15 19 4 823.36 0.07 56 15 168 153 93.90 0.03 57 16 19 3 851.45 0.86 58 16 20 4 823.40 0.10 59 17 20 3 845.98 3.60 60 17 21 4 823.40 0.10 61 17 52 35 114.75 0.69 62 17 158 141 100.22 0.92 63 18 21 3 832.20 4.29 64 18 22 4 825.99 1.49 65 18 157 139 104.28 0.54 66 18 158 140 100.22 0.91 67 18 161 143 99.42 1.29 68 18 162 144 98.14 0.67 69 18 168 150 100.36 0.84 70 19 22 3 841.26 4.99 71 19 23 4 826.00 1.49 72 20 23 3 844.52 7.78 73 20 24 4 825.99 1.49 74 21 24 3 850.50 0.39 75 21 25 4 840.11 5.58 76 21 50 29 116.94 0.60 77 21 64 43 115.92 0.34 78 21 158 137 116.16 0.32 79 21 162 141 112.28 0.42 80 22 25 3 850.50 0.39 81 22 161 139 116.59 0.34 82 22 162 140 114.17 0.80 83 22 164 142 116.05 0.12 84 22 165 143 116.61 0.32 85 22 168 146 116.97 0.46 86 22 238 216 111.14 0.03 87 22 239 217 108.75 0.35 88 25 37 12 116.82 0.51 89 25 64 39 117.12 0.62 90 25 66 41 117.00 0.57 91 25 69 44 117.11 0.61 92 25 239 214 117.17 0.58 93 26 38 12 117.12 0.66 94 26 41 15 117.16 0.64 95 26 66 40 117.15 0.64 96 27 30 3 936.05 1.10 97 27 35 8 117.25 0.61 98 27 36 9 117.19 0.62 99 27 38 11 117.14 0.67 100 28 38 10 117.24 0.62 101 29 35 6 191.18 35.55 102 29 36 7 117.59 0.79 103 30 33 3 846.10 2.94 104 30 34 4 511.66 104.52 105 30 35 5 191.00 35.63 106 30 36 6 118.12 0.68 107 31 34 3 832.11 5.12 108 31 36 5 337.42 105.82 109 31 199 168 156.06 1.14 110 31 200 169 147.31 1.77 111 31 203 172 161.27 0.37 112 31 240 209 128.64 0.45 113 32 199 167 161.39 0.33 114 32 202 170 161.99 0.42 115 32 203 171 161.99 0.42 116 32 207 175 162.18 0.52 117 32 228 196 162.13 0.52 118 34 199 165 162.36 0.57 119 34 233 199 162.40 0.56 120 34 237 203 162.38 0.56 121 34 238 204 162.22 0.60 122 34 239 205 162.21 0.59 123 34 240 206 162.19 0.59 124 35 238 203 162.41 0.56 125 35 239 204 162.28 0.58 126 35 240 205 162.26 0.58 127 36 200 164 162.39 0.56 128 36 240 204 162.28 0.57 129 36 242 206 161.31 0.60 130 37 66 29 162.37 0.55 131 37 67 30 162.37 0.55 132 37 68 31 162.37 0.55 133 37 69 32 162.38 0.54 134 37 192 155 162.43 0.56 135 37 239 202 162.44 0.56 136 37 240 203 162.36 0.56 137 37 241 204 162.27 0.58 138 37 242 205 162.10 0.63 139 38 41 3 918.89 4.32 140 38 67 29 409.16 122.81 141 38 242 204 162.29 0.59 142 39 242 203 162.21 0.64 143 39 244 205 161.88 0.68 144 39 245 206 145.00 1.33 145 40 67 27 654.46 0.48 146 41 45 4 818.65 3.13 147 41 46 5 737.31 2.23 148 41 66 25 727.34 2.69 149 41 67 26 721.39 7.79 150 42 45 3 844.61 0.68 151 42 46 4 819.42 3.52 152 43 46 3 836.22 4.88 153 43 47 4 836.22 4.88 154 43 94 51 653.70 0.67 155 45 66 21 735.72 2.98 156 46 65 19 735.74 2.96 157 46 66 20 735.67 2.99 158 46 67 21 735.65 3.00 159 46 90 44 690.81 2.13 160 46 94 48 690.71 2.17 161 47 64 17 735.77 2.96 162 47 65 18 735.75 2.96 163 47 66 19 735.75 2.96 164 47 72 25 734.62 3.17 165 47 90 43 691.22 2.31 166 47 91 44 690.79 2.14 167 47 94 47 690.73 2.16 168 47 95 48 690.61 2.12 169 48 62 14 736.11 2.98 170 48 63 15 736.00 2.98 171 48 64 16 735.85 2.97 172 48 66 18 735.75 2.96 173 49 62 13 736.08 2.96 174 49 63 14 736.00 2.98 175 49 95 46 691.40 2.38 176 49 96 47 691.28 2.37 177 50 61 11 736.27 2.98 178 50 62 12 736.12 2.98 179 50 64 14 736.11 2.98 180 50 154 104 499.31 0.49 181 50 158 108 444.86 1.29 182 51 59 8 736.66 3.08 183 51 60 9 736.46 2.98 184 51 61 10 736.27 2.98 185 52 59 7 736.68 3.08 186 52 60 8 736.47 2.98 187 52 62 10 736.38 3.02 188 52 132 80 543.09 0.00 189 52 136 84 542.90 0.04 190 52 154 102 507.10 1.77 191 52 158 106 467.19 0.66 192 53 57 4 769.39 13.23 193 53 58 5 737.51 2.71 194 53 59 6 736.69 3.08 195 53 136 83 543.00 0.03 196 54 57 3 818.15 3.34 197 54 58 4 737.53 2.71 198 54 133 79 543.07 0.03 199 54 136 82 543.01 0.04 200 54 137 83 543.00 0.03 201 55 130 75 543.32 0.02 202 55 133 78 543.19 0.01 203 58 136 78 543.02 0.04 204 58 140 82 542.96 0.03 205 58 141 83 542.96 0.03 206 59 140 81 542.92 0.04 207 60 74 14 734.84 2.90 208 60 75 15 734.54 2.94 209 60 76 16 734.26 3.04 210 60 136 76 542.80 0.01 211 60 139 79 542.90 0.04 212 60 140 80 542.78 0.02 213 60 154 94 510.35 0.14 214 61 74 13 734.55 2.94 215 61 75 14 734.52 2.94 216 61 96 35 691.87 2.52 217 62 71 9 735.42 2.92 218 62 73 11 734.78 3.04 219 62 74 12 734.31 3.04 220 62 96 34 691.85 2.53 221 62 154 92 499.31 0.50 222 63 71 8 735.27 2.99 223 63 72 9 735.09 3.06 224 63 73 10 734.64 3.00 225 63 95 32 691.47 2.43 226 63 96 33 691.48 2.43 227 64 69 5 735.57 2.96 228 64 70 6 735.29 2.98 229 64 71 7 734.97 3.12 230 65 68 3 865.59 4.83 231 65 69 4 741.21 0.55 232 65 70 5 735.75 3.27 233 65 72 7 734.75 3.09 234 67 241 174 179.97 6.25 235 67 245 178 161.55 0.71 236 68 163 95 444.23 1.45 237 68 192 124 283.54 1.17 238 68 241 173 179.84 6.18 239 68 245 177 161.27 0.48 240 69 159 90 444.56 1.29 241 69 162 93 444.24 1.45 242 69 163 94 444.23 1.45 243 69 192 123 286.16 0.05 244 69 239 170 196.96 3.02 245 70 85 15 692.72 2.62 246 70 163 93 444.30 1.47 247 71 83 12 692.84 2.56 248 71 154 83 498.44 0.60 249 71 155 84 487.67 1.29 250 71 158 87 444.97 1.26 251 71 159 88 444.85 1.29 252 72 82 10 692.85 2.56 253 72 83 11 692.81 2.55 254 72 85 13 692.71 2.59 255 72 87 15 692.44 2.54 256 72 88 16 691.92 2.53 257 72 91 19 691.92 2.52 258 73 81 8 692.89 2.56 259 73 82 9 692.84 2.56 260 73 96 23 691.89 2.52 261 74 80 6 693.09 2.55 262 74 81 7 693.00 2.60 263 74 96 22 691.90 2.52 264 74 139 65 542.63 0.06 265 74 142 68 542.46 0.03 266 74 151 77 530.75 0.34 267 75 79 4 743.50 2.02 268 75 80 5 693.23 2.59 269 75 96 21 691.89 2.52 270 75 98 23 691.88 2.53 271 75 99 24 691.18 2.31 272 76 79 3 855.36 1.97 273 76 81 5 772.82 12.60 274 76 140 64 542.72 0.01 275 78 99 21 691.37 2.35 276 78 100 22 691.21 2.30 277 78 101 23 691.20 2.31 278 79 99 20 691.24 2.29 279 79 101 22 690.99 2.25 280 79 103 24 678.99 0.51 281 80 96 16 691.91 2.52 282 80 99 19 691.18 2.31 283 80 101 21 690.70 2.12 284 80 102 22 652.21 0.85 285 80 103 23 639.33 4.75 286 81 103 22 569.19 6.48 287 81 105 24 552.15 1.82 288 81 142 61 542.27 0.05 289 82 88 6 692.50 2.51 290 82 91 9 692.45 2.54 291 82 92 10 692.33 2.51 292 82 95 13 692.21 2.42 293 82 96 14 692.09 2.46 294 82 102 20 645.29 7.73 295 82 103 21 602.50 23.14 296 82 104 22 552.15 1.82 297 82 105 23 552.12 1.83 298 83 88 5 731.31 16.89 299 83 104 21 552.19 1.81 300 83 105 22 552.09 1.83 301 83 107 24 550.09 2.73 302 83 108 25 544.37 0.17 303 83 109 26 544.21 0.24 304 83 151 68 537.90 0.53 305 84 104 20 552.17 1.83 306 84 105 21 552.14 1.83 307 84 107 23 548.85 1.53 308 84 108 24 544.37 0.18 309 85 88 3 842.39 0.49 310 85 89 4 831.97 2.98 311 85 108 23 544.37 0.18 312 86 89 3 840.15 0.46 313 86 90 4 824.99 1.48 314 87 90 3 842.70 0.82 315 87 91 4 834.67 2.17 316 88 91 3 840.14 0.46 317 88 92 4 850.21 4.57 318 89 92 3 850.22 4.57 319 89 93 4 850.23 4.55 320 90 93 3 855.22 2.06 321 90 94 4 850.23 4.55 322 91 94 3 853.25 3.05 323 91 95 4 834.55 3.27 324 92 102 10 602.53 23.16 325 93 97 4 847.99 3.29 326 94 97 3 880.09 0.65 327 96 99 3 856.06 4.21 328 105 142 37 542.29 0.06 329 105 151 46 539.62 0.14 330 107 113 6 618.57 36.93 331 107 114 7 550.27 2.68 332 107 117 10 544.82 0.05 333 107 147 40 542.04 0.03 334 107 148 41 541.53 0.04 335 107 151 44 541.19 0.17 336 109 114 5 822.67 1.74 337 110 113 3 854.83 3.56 338 110 114 4 830.37 5.58 339 111 114 3 837.45 5.05 340 111 115 4 822.69 1.74 341 111 122 11 544.86 0.01 342 111 152 41 541.71 0.04 343 111 155 44 540.45 0.15 344 111 156 45 540.44 0.16 345 111 160 49 502.51 3.11 346 111 189 78 300.06 1.19 347 112 115 3 845.98 0.86 348 112 116 4 837.42 5.07 349 113 116 3 842.57 2.50 350 113 117 4 834.38 3.56 351 114 117 3 845.88 0.84 352 114 118 4 834.38 3.56 353 114 148 34 542.04 0.04 354 114 151 37 541.88 0.03 355 114 152 38 541.69 0.01 356 114 155 41 540.79 0.01 357 115 118 3 836.05 2.73 358 115 119 4 818.99 1.49 359 115 120 5 791.60 6.51 360 115 121 6 749.01 8.07 361 115 122 7 749.00 8.07 362 115 152 37 541.84 0.01 363 116 119 3 826.13 0.58 364 117 148 31 542.25 0.04 365 118 148 30 542.25 0.04 366 118 149 31 542.19 0.01 367 118 152 34 542.11 0.01 368 120 124 4 820.78 1.97 369 120 125 5 812.99 3.49 370 120 126 6 820.35 1.75 371 121 124 3 853.62 2.49 372 121 125 4 849.89 4.34 373 122 125 3 851.23 3.69 374 122 152 30 541.82 0.01 375 122 156 34 540.73 0.04 376 122 181 59 429.25 1.06 377 122 186 64 367.88 2.46 378 122 189 67 354.42 6.53 379 122 190 68 357.39 8.02 380 123 180 57 429.49 0.97 381 123 181 58 429.25 1.06 382 123 183 60 424.62 1.72 383 123 186 63 391.98 0.70 384 124 180 56 429.49 1.05 385 125 149 24 542.00 0.06 386 125 152 27 541.70 0.03 387 125 153 28 541.12 0.17 388 125 180 55 429.42 1.03 389 125 181 56 429.41 1.03 390 126 131 5 811.53 1.72 391 126 153 27 540.76 0.01 392 126 178 52 430.23 1.12 393 126 179 53 430.13 1.12 394 126 180 54 429.39 1.02 395 127 131 4 812.72 1.16 396 127 153 26 540.23 0.11 397 127 156 29 538.60 0.33 398 127 171 44 444.15 1.54 399 127 178 51 430.21 1.13 400 127 179 52 430.06 1.15 401 127 181 54 428.62 0.95 402 128 131 3 846.85 7.15 403 128 132 4 846.84 7.15 404 128 171 43 444.04 1.58 405 128 174 46 439.52 0.24 406 128 178 50 430.23 1.12 407 128 179 51 430.19 1.12 408 129 132 3 846.85 7.15 409 129 133 4 840.73 0.29 410 129 171 42 444.04 1.58 411 129 172 43 443.93 1.57 412 129 174 45 440.08 1.01 413 129 175 46 437.44 0.15 414 130 133 3 840.77 0.31 415 130 134 4 834.80 2.68 416 130 174 44 442.24 1.60 417 130 175 45 437.55 0.18 418 130 176 46 434.93 0.88 419 130 177 47 432.39 0.23 420 130 178 48 430.23 1.12 421 131 134 3 843.70 7.12 422 131 135 4 834.79 2.68 423 131 153 22 539.23 0.25 424 131 178 47 430.23 1.12 425 132 135 3 835.96 2.10 426 132 136 4 834.80 2.68 427 132 153 21 538.57 0.35 428 132 154 22 538.43 0.36 429 132 157 25 509.92 0.30 430 133 136 3 849.78 4.81 431 133 137 4 834.80 2.68 432 134 137 3 831.56 0.77 433 134 138 4 832.15 1.06 434 135 138 3 854.51 3.63 435 135 139 4 831.88 1.20 436 135 150 15 539.59 0.07 437 135 153 18 538.23 0.40 438 136 139 3 853.22 2.66 439 136 140 4 819.26 0.87 440 137 140 3 844.47 1.39 441 137 141 4 822.52 0.76 442 138 141 3 840.74 0.48 443 138 142 4 822.51 0.76 444 138 146 8 541.54 0.00 445 138 150 12 540.11 0.14 446 139 142 3 822.52 0.76 447 139 147 8 540.59 0.29 448 139 150 11 539.71 0.13 449 139 151 12 539.64 0.18 450 139 154 15 522.12 2.12 451 142 147 5 541.44 0.04 452 143 146 3 860.90 0.99 453 143 147 4 754.22 1.00 454 146 149 3 876.87 3.21 455 146 150 4 832.61 4.49 456 147 150 3 843.52 0.97 457 147 151 4 832.61 4.48 458 148 151 3 834.62 3.48 459 148 152 4 832.62 4.49 460 149 152 3 838.91 1.33 461 149 153 4 834.61 3.47 462 150 153 3 838.89 1.32 463 150 154 4 820.39 0.22 464 151 154 3 824.44 2.54 465 151 155 4 820.10 0.37 466 152 155 3 826.87 3.77 467 152 156 4 823.07 1.87 468 153 156 3 837.13 1.36 469 153 157 4 833.34 3.25 470 154 157 3 841.05 3.33 471 154 158 4 833.33 3.26 472 155 158 3 848.55 3.40 473 155 159 4 819.11 0.18 474 155 160 5 776.25 1.97 475 156 160 4 773.04 3.52 476 156 161 5 803.59 2.96 477 156 167 11 444.17 1.53 478 156 171 15 444.09 1.51 479 156 189 33 287.75 0.03 480 157 161 4 814.62 8.30 481 157 171 14 443.98 1.56 482 158 161 3 839.30 0.81 483 158 162 4 818.73 6.38 484 159 162 3 833.72 0.24 485 159 163 4 825.13 3.18 486 160 163 3 826.05 2.72 487 160 164 4 819.73 5.88 488 160 167 7 570.82 9.60 489 160 189 29 287.71 0.04 490 161 164 3 837.19 1.08 491 161 167 6 444.18 1.53 492 161 168 7 444.01 1.53 493 162 238 76 203.13 2.19 494 162 239 77 198.61 2.50 495 163 192 29 286.23 0.04 496 163 237 74 203.54 2.03 497 163 238 75 202.65 2.44 498 163 239 76 197.69 2.66 499 164 167 3 839.23 0.30 500 164 168 4 834.21 0.35 501 164 188 24 294.36 3.27 502 164 191 27 294.00 3.16 503 164 192 28 287.26 0.03 504 164 195 31 287.06 0.07 505 164 232 68 204.51 1.66 506 164 236 72 203.97 1.89 507 164 237 73 203.81 1.90 508 165 168 3 835.24 0.87 509 165 169 4 834.23 0.35 510 165 235 70 204.23 1.78 511 165 236 71 204.09 1.85 512 165 238 73 203.90 1.94 513 166 169 3 859.05 7.29 514 166 170 4 834.23 0.35 515 166 184 18 325.31 6.45 516 166 185 19 321.42 4.50 517 166 188 22 308.42 1.38 518 166 236 70 204.19 1.80 519 167 170 3 847.48 4.33 520 167 171 4 834.23 0.34 521 167 185 18 302.23 1.73 522 167 188 21 297.42 4.12 523 167 189 22 296.91 3.86 524 168 171 3 848.74 2.52 525 168 172 4 844.19 3.58 526 169 172 3 846.58 3.60 527 169 173 4 842.46 4.46 528 170 173 3 859.56 0.24 529 170 174 4 844.20 3.58 530 170 179 9 430.16 1.12 531 170 185 15 373.19 6.53 532 171 174 3 864.65 2.79 533 171 179 8 430.17 1.12 534 172 175 3 866.74 1.62 535 174 177 3 827.20 2.14 536 174 178 4 776.63 12.19 537 174 179 5 762.64 5.29 538 179 185 6 834.30 1.07 539 181 185 4 822.40 4.87 540 181 186 5 822.40 4.87 541 182 185 3 846.62 4.83 542 182 186 4 835.03 0.71 543 183 186 3 831.26 1.04 544 183 187 4 830.20 0.97 545 183 216 33 264.11 6.84 546 183 220 37 264.10 6.90 547 184 187 3 835.61 0.43 548 184 188 4 830.22 0.97 549 184 216 32 264.16 6.85 550 184 217 33 264.43 6.72 551 185 188 3 849.14 2.00 552 185 189 4 837.11 0.35 553 186 189 3 837.11 0.35 554 186 190 4 831.18 0.27 555 187 190 3 839.93 4.09 556 187 191 4 831.19 0.28 557 187 215 28 264.56 6.66 558 187 216 29 264.56 6.67 559 188 191 3 842.68 2.72 560 188 192 4 830.01 0.85 561 188 215 27 264.59 6.68 562 188 232 44 205.43 1.53 563 188 236 48 204.33 1.83 564 189 192 3 864.78 3.54 565 191 194 3 848.98 4.36 566 191 195 4 824.19 4.21 567 191 213 22 265.96 6.45 568 191 215 24 265.88 6.34 569 192 195 3 846.41 4.61 570 192 196 4 830.25 1.18 571 192 237 45 203.82 2.06 572 192 239 47 203.58 2.12 573 192 240 48 200.00 3.75 574 192 241 49 196.94 3.02 575 193 196 3 833.04 0.31 576 193 197 4 822.12 5.33 577 193 241 48 193.47 2.09 578 193 246 53 175.67 3.19 579 194 197 3 831.46 1.10 580 194 198 4 823.74 5.87 581 194 213 19 267.74 6.10 582 195 198 3 856.90 3.04 583 195 199 4 832.35 1.56 584 195 209 14 269.30 6.75 585 195 213 18 267.87 6.04 586 195 215 20 267.88 6.03 587 195 225 30 267.87 6.04 588 195 232 37 205.63 1.62 589 195 237 42 203.91 2.06 590 196 199 3 857.34 3.26 591 196 200 4 836.12 0.33 592 196 240 44 202.16 2.68 593 196 241 45 202.16 2.68 594 196 246 50 183.49 4.45 595 197 200 3 857.12 3.37 596 197 201 4 836.13 0.33 597 197 246 49 183.53 4.42 598 198 201 3 843.60 3.39 599 198 202 4 834.96 0.92 600 198 209 11 269.31 6.75 601 198 210 12 269.31 6.75 602 198 211 13 269.24 6.70 603 198 213 15 268.06 6.09 604 199 202 3 836.10 0.35 605 199 203 4 834.96 0.91 606 199 209 10 269.31 6.75 607 199 233 34 237.37 0.97 608 199 237 38 204.51 2.02 609 199 240 41 203.77 2.12 610 200 203 3 846.93 4.63 611 200 204 4 837.20 0.23 612 200 241 41 203.69 2.15 613 200 242 42 203.60 2.12 614 200 243 43 202.53 2.50 615 201 204 3 837.19 0.24 616 201 205 4 834.89 0.90 617 201 210 9 269.36 6.75 618 202 205 3 834.88 0.91 619 202 206 4 823.15 3.48 620 202 207 5 534.38 106.82 621 202 208 6 285.27 1.43 622 202 209 7 271.45 5.70 623 202 228 26 267.95 6.07 624 205 208 3 849.51 7.18 625 205 209 4 837.60 2.49 626 207 227 20 268.88 5.69 627 207 228 21 268.13 6.04 628 208 226 18 268.98 5.66 629 208 227 19 268.86 5.68 630 209 213 4 852.51 3.42 631 209 225 16 269.13 5.58 632 209 226 17 268.95 5.63 633 209 227 18 268.94 5.64 634 209 228 19 268.94 5.64 635 209 233 24 246.84 5.41 636 212 224 12 269.41 5.57 637 212 225 13 269.26 5.61 638 212 226 14 269.13 5.58 639 213 224 11 269.49 5.58 640 213 225 12 269.31 5.59 641 214 222 8 269.94 5.58 642 214 223 9 269.69 5.50 643 214 224 10 269.50 5.57 644 215 222 7 269.95 5.58 645 215 223 8 269.89 5.59 646 215 225 10 269.68 5.50 647 215 232 17 233.56 12.18 648 216 220 4 295.27 4.80 649 216 221 5 280.55 10.81 650 216 222 6 270.00 5.56 651 217 220 3 827.21 5.91 652 217 221 4 295.26 4.80 653 217 223 6 270.01 5.55 654 225 230 5 313.84 1.81 655 225 232 7 312.48 2.60 656 227 230 3 863.82 6.25 657 227 233 6 322.79 5.03 658 228 233 5 322.78 5.03 659 228 234 6 322.79 5.04 660 229 234 5 500.78 83.94 661 230 233 3 847.62 2.02 662 230 234 4 806.90 9.11 663 231 234 3 830.37 2.63 664 231 235 4 800.09 12.50 665 231 236 5 721.89 26.61 666 232 236 4 721.89 26.61 667 232 237 5 472.69 79.93 668 233 237 4 721.89 26.61 669 237 240 3 932.66 1.02 670 241 245 4 830.65 0.65 671 241 246 5 814.22 7.56 672 242 245 3 836.88 2.58 673 242 246 4 843.99 0.97 674 243 246 3 868.96 6.75