BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 8 128 121 27.55 0.07 2 9 127 119 45.91 0.89 3 10 127 118 46.67 1.17 4 10 128 119 46.70 1.16 5 11 14 3 843.75 3.98 6 11 125 115 46.73 1.17 7 11 126 116 46.73 1.17 8 11 127 117 46.80 1.12 9 12 23 11 219.53 87.49 10 12 128 117 46.80 1.12 11 12 129 118 46.80 1.12 12 13 21 8 219.80 87.61 13 13 23 10 219.53 87.48 14 13 24 11 219.54 87.49 15 13 124 112 46.81 1.13 16 13 131 119 46.81 1.12 17 14 21 7 219.87 87.60 18 15 20 5 379.83 124.05 19 15 21 6 219.87 87.60 20 16 19 3 793.15 5.46 21 16 20 4 627.89 0.05 22 20 25 5 793.57 9.83 23 21 24 3 834.86 0.54 24 21 25 4 808.88 2.18 25 22 25 3 834.86 0.54 26 22 26 4 808.89 2.18 27 23 26 3 828.08 3.93 28 23 27 4 808.89 2.18 29 23 131 109 357.45 6.60 30 24 27 3 813.58 3.33 31 24 28 4 808.88 2.18 32 24 124 101 357.45 6.59 33 24 131 108 357.48 6.59 34 24 154 132 356.14 6.12 35 25 28 3 821.97 0.86 36 25 29 4 804.88 0.18 37 26 29 3 822.95 7.49 38 26 30 4 804.89 0.17 39 27 30 3 814.76 1.67 40 27 31 4 804.80 0.21 41 27 131 105 360.12 5.82 42 27 132 106 360.12 5.82 43 27 133 107 360.12 5.82 44 27 153 128 359.89 5.91 45 28 31 3 812.59 0.57 46 28 32 4 804.85 0.27 47 28 153 127 359.93 5.91 48 29 32 3 809.56 2.08 49 29 33 4 809.48 2.12 50 30 33 3 822.81 4.53 51 30 34 4 813.03 0.35 52 30 81 51 360.90 5.87 53 31 34 3 820.96 1.45 54 31 79 48 360.73 5.88 55 31 80 49 360.73 5.88 56 31 133 103 360.73 5.88 57 31 134 104 360.63 5.88 58 31 135 105 360.48 5.88 59 31 153 124 360.62 5.87 60 31 166 137 340.12 0.85 61 32 166 136 340.14 0.84 62 32 167 137 339.80 0.84 63 33 167 136 339.96 0.77 64 34 80 46 361.26 5.70 65 34 166 134 340.82 1.11 66 34 167 135 339.96 0.77 67 35 51 16 407.73 14.14 68 35 53 18 407.72 14.14 69 35 66 31 407.06 13.85 70 35 79 44 373.80 8.14 71 36 50 14 407.74 14.14 72 36 51 15 407.84 14.12 73 36 79 43 398.76 10.44 74 36 164 130 341.69 1.05 75 36 165 131 341.53 1.09 76 36 166 132 340.92 1.14 77 36 167 133 340.91 1.14 78 36 168 134 340.83 1.11 79 36 197 163 257.08 1.12 80 36 200 166 256.77 1.04 81 37 49 12 407.88 14.10 82 37 50 13 407.74 14.15 83 37 77 40 405.15 12.96 84 37 78 41 399.34 10.15 85 37 79 42 398.78 10.44 86 37 137 101 404.88 12.78 87 37 164 129 341.69 1.05 88 37 165 130 341.61 1.07 89 38 48 10 407.89 14.11 90 38 49 11 407.89 14.11 91 38 137 100 407.21 13.91 92 38 163 127 342.02 1.18 93 38 164 128 341.70 1.05 94 38 193 157 260.05 0.74 95 38 196 160 259.50 0.54 96 38 197 161 259.42 0.49 97 39 47 8 414.33 11.01 98 39 48 9 407.91 14.11 99 39 137 99 407.81 14.13 100 39 148 111 407.86 14.12 101 39 162 125 352.96 6.52 102 39 163 126 346.01 3.07 103 40 46 6 420.22 12.40 104 40 47 7 414.38 11.02 105 40 148 110 407.95 14.10 106 40 161 123 361.08 5.67 107 40 162 124 354.70 7.08 108 40 176 138 327.82 0.98 109 40 180 142 309.00 0.85 110 40 189 151 260.69 0.84 111 40 192 154 260.50 0.85 112 40 193 155 260.14 0.80 113 41 45 4 759.24 5.16 114 41 46 5 415.92 10.25 115 41 147 107 408.32 14.07 116 41 147 108 408.14 14.13 117 41 148 109 407.98 14.10 118 41 160 121 361.88 5.61 119 41 161 122 361.12 5.66 120 41 180 141 310.94 1.62 121 42 45 3 795.65 3.72 122 42 184 144 271.87 2.29 123 42 185 145 271.83 2.28 124 42 186 146 261.99 0.43 125 42 189 149 260.87 0.76 126 43 182 141 308.88 0.85 127 43 183 142 293.33 3.60 128 43 184 143 280.73 3.39 129 44 147 104 417.35 12.95 130 44 185 143 271.89 2.29 131 44 186 144 271.83 2.28 132 45 147 103 417.40 12.96 133 45 186 143 261.72 0.58 134 45 215 172 255.41 0.83 135 45 216 173 255.11 0.81 136 46 139 94 417.22 13.00 137 46 147 102 417.44 12.95 138 46 147 103 417.44 12.96 139 46 148 104 417.32 12.95 140 46 186 142 261.71 0.58 141 46 214 170 255.36 0.85 142 46 215 171 255.18 0.79 143 47 186 141 261.58 0.64 144 47 189 144 260.83 0.78 145 47 190 145 260.65 0.79 146 47 193 148 260.23 0.70 147 47 212 167 255.91 0.82 148 47 213 168 255.53 0.85 149 47 214 169 255.16 0.80 150 48 67 19 417.76 12.97 151 48 75 27 417.76 12.98 152 48 76 28 417.74 12.97 153 48 77 29 417.69 12.96 154 48 137 90 417.67 12.98 155 48 139 92 417.71 12.98 156 48 148 102 417.70 12.98 157 48 212 166 255.60 0.87 158 48 213 167 255.40 0.82 159 49 197 150 259.77 0.62 160 49 211 164 255.85 0.84 161 49 212 165 255.31 0.81 162 50 66 16 417.78 12.97 163 50 67 17 417.84 12.99 164 50 77 27 417.78 12.98 165 50 197 149 259.44 0.51 166 50 210 162 255.91 0.82 167 50 211 163 255.63 0.94 168 51 66 15 417.87 13.00 169 51 197 148 259.73 0.65 170 51 202 152 257.00 1.11 171 51 203 153 257.00 1.11 172 51 209 160 255.99 0.81 173 51 210 161 255.92 0.82 174 52 61 9 418.06 12.90 175 52 209 158 256.02 0.81 176 52 209 159 255.96 0.80 177 52 210 160 255.93 0.81 178 52 211 161 255.89 0.80 179 53 203 151 257.02 1.10 180 56 59 3 803.62 6.47 181 60 63 3 828.81 3.66 182 61 64 3 838.53 1.61 183 61 209 149 256.00 0.82 184 65 211 148 255.69 0.91 185 65 223 175 114.30 3.56 186 66 77 11 509.99 1.02 187 66 78 12 509.98 1.01 188 66 85 19 509.92 0.98 189 66 211 147 255.56 0.84 190 67 76 9 576.20 19.32 191 67 77 10 516.36 4.17 192 67 85 18 531.58 3.29 193 67 211 146 255.84 0.84 194 67 213 148 255.37 0.81 195 67 221 171 118.06 3.88 196 67 223 173 112.05 2.45 197 68 75 7 579.24 19.22 198 68 76 8 577.11 18.93 199 68 85 17 539.85 0.25 200 68 89 21 539.49 0.59 201 68 93 25 540.92 0.24 202 68 221 170 118.06 3.88 203 69 72 3 863.86 0.83 204 69 74 5 581.24 19.91 205 69 75 6 579.25 19.21 206 69 93 24 540.97 0.27 207 69 221 169 118.01 3.86 208 70 74 4 702.94 15.27 209 70 76 6 581.22 19.91 210 70 89 19 540.91 0.24 211 70 92 22 540.92 0.24 212 70 93 23 540.92 0.24 213 70 112 43 577.99 18.29 214 70 138 69 540.96 0.26 215 71 74 3 818.02 6.62 216 71 96 25 597.41 9.14 217 71 106 36 612.55 1.72 218 71 107 37 612.39 1.80 219 71 112 42 595.53 10.14 220 72 106 35 613.25 1.42 221 72 219 164 118.17 3.98 222 72 220 165 118.22 3.95 223 72 221 166 118.17 3.92 224 73 140 68 608.94 0.77 225 73 141 69 608.86 0.81 226 73 215 144 255.50 0.83 227 73 219 163 118.24 3.97 228 73 220 164 118.21 3.95 229 74 107 34 613.48 1.46 230 74 108 35 613.47 1.46 231 74 109 36 613.33 1.46 232 74 112 39 613.26 1.43 233 74 138 65 609.24 0.78 234 74 139 66 609.13 0.81 235 74 140 67 608.97 0.79 236 74 215 143 255.36 0.86 237 75 138 64 609.24 0.78 238 75 139 65 609.21 0.80 239 75 215 142 255.18 0.77 240 75 221 163 117.95 3.89 241 76 85 9 614.63 1.45 242 76 137 62 609.44 0.80 243 76 138 63 609.24 0.79 244 77 85 8 614.64 1.45 245 77 136 60 609.52 0.79 246 77 137 61 609.47 0.79 247 78 82 4 821.06 2.81 248 78 85 7 628.97 4.56 249 78 134 57 611.19 0.26 250 78 135 58 609.81 0.74 251 78 136 59 609.55 0.81 252 78 165 89 341.05 1.07 253 79 134 56 612.25 0.24 254 79 135 57 610.16 0.78 255 79 165 88 340.90 1.14 256 79 166 89 340.55 1.04 257 80 133 54 612.80 0.03 258 80 134 55 612.25 0.24 259 81 84 3 837.27 3.60 260 81 85 4 814.12 3.49 261 81 130 50 617.86 2.15 262 81 133 53 614.48 0.81 263 81 134 54 614.54 0.77 264 82 85 3 819.57 0.77 265 82 86 4 814.28 3.41 266 83 86 3 819.57 0.77 267 83 87 4 816.64 2.23 268 84 87 3 837.72 2.55 269 84 88 4 814.31 3.40 270 84 130 47 618.00 2.19 271 84 132 49 616.41 1.62 272 84 133 50 616.39 1.63 273 84 134 51 616.36 1.64 274 85 88 3 810.57 1.53 275 85 89 4 810.50 1.50 276 85 134 50 616.06 1.79 277 85 136 52 613.98 2.83 278 86 89 3 819.28 1.29 279 86 90 4 815.09 0.80 280 87 90 3 813.93 0.21 281 87 91 4 808.88 0.73 282 87 132 46 617.98 2.21 283 88 91 3 823.09 0.48 284 88 92 4 807.41 0.00 285 88 116 29 619.68 1.45 286 88 119 32 619.69 1.45 287 88 120 33 619.44 1.57 288 88 132 45 617.77 2.28 289 88 134 47 617.59 2.27 290 88 136 49 614.60 3.18 291 89 92 3 806.30 0.57 292 89 93 4 806.27 0.58 293 90 93 3 819.10 0.24 294 90 94 4 813.89 0.26 295 91 94 3 824.45 5.00 296 91 95 4 804.73 4.84 297 91 119 29 619.74 1.46 298 92 95 3 832.97 3.06 299 92 96 4 813.93 0.26 300 92 112 21 619.95 1.49 301 92 116 25 619.75 1.46 302 93 96 3 818.13 1.84 303 93 97 4 813.94 0.25 304 94 97 3 828.53 3.35 305 94 98 4 804.71 4.86 306 95 98 3 809.27 6.29 307 95 99 4 795.80 0.45 308 95 112 18 620.03 1.47 309 95 115 21 619.89 1.47 310 96 100 4 804.30 8.77 311 96 101 5 763.57 4.75 312 96 107 12 620.95 1.56 313 96 112 17 620.01 1.46 314 97 100 3 830.29 4.23 315 97 101 4 743.41 8.11 316 97 102 5 702.70 1.54 317 97 106 10 622.80 1.71 318 98 102 4 739.60 19.98 319 100 102 3 805.35 5.09 320 100 103 4 754.25 12.66 321 100 105 6 655.91 2.77 322 100 106 7 624.34 0.96 323 100 107 8 622.66 1.62 324 100 108 9 622.85 1.71 325 100 111 12 621.83 1.68 326 101 103 3 823.68 0.92 327 101 104 4 649.61 3.04 328 101 105 5 637.78 0.72 329 101 106 6 622.83 1.70 330 107 112 5 763.84 5.57 331 108 111 3 837.21 0.83 332 108 112 4 809.27 1.57 333 108 142 34 613.14 2.94 334 109 112 3 821.24 7.24 335 109 113 4 798.97 6.72 336 109 138 29 614.84 3.52 337 109 140 31 613.89 3.14 338 109 141 32 613.78 3.09 339 109 142 33 613.66 3.05 340 109 147 39 608.95 0.85 341 109 149 41 608.89 0.86 342 110 113 3 817.96 2.04 343 110 114 4 806.55 0.08 344 110 145 35 609.47 0.96 345 110 149 40 608.95 0.85 346 111 114 3 812.22 2.91 347 111 115 4 813.02 2.56 348 112 115 3 816.03 1.05 349 112 116 4 816.02 1.05 350 113 116 3 816.47 1.27 351 113 117 4 816.02 1.05 352 113 138 25 614.87 3.50 353 113 149 37 608.94 0.84 354 113 151 39 608.76 0.82 355 113 157 45 493.16 6.79 356 114 117 3 819.77 0.82 357 114 118 4 816.02 1.05 358 114 157 44 494.08 6.36 359 115 118 3 815.95 2.73 360 115 119 4 819.97 0.72 361 116 119 3 819.97 0.72 362 116 120 4 820.42 0.89 363 116 136 20 615.23 3.51 364 116 138 22 614.47 3.23 365 117 120 3 825.57 1.69 366 117 121 4 820.43 0.89 367 117 151 35 608.89 0.86 368 117 155 39 517.35 5.76 369 117 157 41 494.07 6.36 370 118 121 3 821.87 0.17 371 118 122 4 820.43 0.89 372 119 122 3 828.24 3.02 373 119 123 4 828.23 3.02 374 119 132 13 618.42 2.44 375 120 123 3 827.85 3.21 376 120 124 4 818.98 0.18 377 120 132 12 618.02 2.38 378 120 134 14 617.96 2.40 379 120 151 32 608.96 0.84 380 120 152 33 608.93 0.84 381 120 153 34 608.69 0.80 382 120 155 36 556.38 2.17 383 121 124 3 820.39 0.89 384 121 125 4 820.39 0.88 385 121 154 34 608.62 0.77 386 121 155 35 596.46 0.27 387 122 125 3 820.38 0.89 388 122 126 4 796.43 5.87 389 123 129 6 618.60 2.46 390 123 130 7 618.55 2.44 391 123 131 8 618.50 2.43 392 123 132 9 618.50 2.43 393 124 131 7 618.50 2.43 394 124 153 30 608.95 0.84 395 124 154 31 608.83 0.82 396 126 129 3 858.09 1.42 397 132 153 22 608.73 0.79 398 134 153 20 608.64 0.79 399 135 151 17 608.75 0.79 400 135 152 18 608.69 0.81 401 135 153 19 608.69 0.81 402 135 165 31 340.91 1.14 403 136 149 14 608.90 0.82 404 136 150 15 608.74 0.80 405 136 151 16 608.73 0.80 406 136 165 30 340.91 1.14 407 137 148 12 608.85 0.80 408 137 149 13 608.75 0.82 409 137 150 14 608.73 0.80 410 137 163 27 341.98 1.17 411 137 165 29 341.44 1.14 412 138 148 11 608.82 0.80 413 138 149 12 608.73 0.82 414 138 151 14 608.71 0.81 415 139 147 9 608.83 0.80 416 139 148 10 608.72 0.82 417 139 215 77 255.16 0.79 418 140 147 7 608.91 0.83 419 140 147 8 608.88 0.83 420 140 215 76 255.28 0.84 421 141 146 5 608.96 0.84 422 141 147 6 608.88 0.84 423 141 215 75 255.39 0.90 424 141 220 95 118.22 3.94 425 142 145 3 825.55 4.38 426 142 146 4 660.47 10.56 427 147 215 69 255.38 0.89 428 147 216 70 255.32 0.86 429 148 159 11 437.03 31.88 430 148 160 12 435.00 30.89 431 148 162 14 400.48 13.93 432 149 157 8 492.00 7.36 433 149 158 9 485.76 10.45 434 149 159 10 436.00 31.39 435 150 157 7 485.88 10.43 436 150 158 8 485.76 10.44 437 150 160 10 484.34 9.96 438 150 165 15 340.92 1.14 439 151 156 5 569.04 31.14 440 151 157 6 485.88 10.42 441 152 155 3 810.06 5.91 442 152 156 4 596.06 33.61 443 152 158 6 587.86 29.50 444 160 163 3 862.50 1.21 445 161 180 19 308.99 0.85 446 162 176 14 327.81 0.98 447 162 180 18 308.99 0.85 448 163 173 10 327.99 0.96 449 163 176 13 327.75 1.01 450 164 169 5 340.12 0.85 451 164 172 8 328.27 1.04 452 164 173 9 327.98 0.96 453 164 176 12 327.75 1.01 454 165 169 4 517.11 52.79 455 166 169 3 809.58 4.59 456 168 171 3 826.60 1.62 457 168 172 4 808.59 0.70 458 169 172 3 809.66 1.24 459 169 173 4 797.14 1.40 460 170 173 3 820.23 1.45 461 170 174 4 803.56 1.81 462 171 174 3 807.63 2.08 463 171 175 4 810.13 5.09 464 172 175 3 810.90 4.71 465 172 176 4 810.81 5.43 466 172 196 24 259.17 0.70 467 173 176 3 821.02 2.91 468 173 177 4 810.65 5.51 469 174 177 3 821.02 2.90 470 174 178 4 818.45 1.62 471 175 178 3 826.24 0.32 472 175 179 4 818.44 1.62 473 175 195 20 260.21 0.93 474 175 196 21 259.47 0.57 475 176 179 3 823.33 0.84 476 176 180 4 796.39 0.89 477 176 192 16 260.41 0.95 478 176 196 20 260.05 0.84 479 179 192 13 260.56 0.91 480 179 195 16 260.56 0.91 481 180 184 4 817.18 3.61 482 181 184 3 846.13 3.23 483 184 188 4 794.86 0.00 484 184 192 8 746.53 4.29 485 185 188 3 817.65 3.55 486 185 189 4 813.41 5.69 487 186 189 3 817.68 3.56 488 186 190 4 802.75 3.90 489 186 214 28 255.93 0.83 490 186 216 30 255.29 0.83 491 187 190 3 809.22 0.68 492 187 191 4 812.93 1.19 493 187 232 60 87.28 0.48 494 188 191 3 813.40 5.70 495 188 192 4 813.39 5.69 496 189 192 3 815.61 4.58 497 189 193 4 813.39 5.69 498 190 193 3 816.23 0.35 499 190 194 4 808.75 3.37 500 190 212 22 255.98 0.82 501 190 214 24 255.69 0.78 502 190 224 49 115.63 3.66 503 190 232 57 83.04 0.39 504 191 194 3 810.99 2.27 505 191 195 4 808.72 3.38 506 191 235 59 75.40 0.54 507 191 236 60 63.82 2.50 508 192 195 3 820.74 1.81 509 192 196 4 812.75 1.36 510 193 196 3 818.02 1.06 511 193 197 4 805.80 1.98 512 193 212 19 255.84 0.83 513 194 197 3 818.72 4.43 514 194 198 4 818.71 4.43 515 194 206 11 331.44 36.81 516 194 210 16 255.99 0.82 517 194 212 18 255.86 0.82 518 194 229 50 88.94 0.84 519 194 232 53 79.20 0.05 520 194 233 54 78.21 0.28 521 195 198 3 821.71 2.94 522 195 199 4 821.72 2.93 523 195 236 56 55.41 0.71 524 196 199 3 832.14 2.28 525 196 200 4 821.74 2.93 526 197 200 3 824.75 4.44 527 197 202 4 821.72 2.94 528 197 206 8 331.41 36.83 529 197 210 13 255.92 0.82 530 198 202 3 822.24 3.18 531 198 206 7 331.36 36.86 532 198 236 53 55.48 0.68 533 202 205 3 916.55 0.70 534 202 206 4 807.98 4.82 535 202 210 9 255.92 0.82 536 205 209 5 731.91 4.33 537 206 209 4 752.14 0.40 538 206 210 5 738.11 7.42 539 206 233 43 78.28 0.25 540 207 209 3 831.38 2.68 541 207 210 4 749.44 10.16 542 207 230 39 88.92 0.85 543 207 233 42 78.29 0.24 544 208 226 34 93.84 0.79 545 208 229 37 89.31 0.66 546 208 230 38 88.94 0.84 547 208 233 41 80.61 0.92 548 209 226 33 94.25 0.71 549 210 229 34 89.28 0.67 550 211 223 27 112.20 2.38 551 212 224 27 112.00 2.46 552 212 229 32 89.31 0.66 553 213 221 23 118.00 3.91 554 213 222 24 117.34 3.82 555 213 223 25 112.20 2.38 556 213 224 26 112.05 2.45 557 214 221 22 118.05 3.89 558 214 222 23 117.76 3.96 559 214 224 25 115.25 3.75 560 215 220 20 118.20 3.95 561 215 221 21 118.00 3.91 562 216 220 19 118.21 3.94 563 217 220 18 118.31 3.96 564 217 217 14 118.67 3.96 565 217 218 15 118.62 3.96 566 217 219 16 118.53 3.94 567 217 220 17 118.33 3.97 568 217 217 3 833.47 2.52 569 217 217 4 817.18 10.66 570 217 218 14 118.69 3.95 571 217 219 15 118.66 3.94 572 217 222 18 118.38 3.99 573 217 217 3 820.14 9.18 574 217 217 12 118.91 3.86 575 217 218 13 118.71 3.95 576 217 219 12 118.83 4.00 577 217 222 15 118.56 3.95 578 217 217 8 120.33 3.50 579 217 217 9 119.25 4.02 580 217 218 10 118.95 3.96 581 217 219 11 118.83 4.01 582 217 217 6 134.03 2.25 583 222 228 6 795.88 1.95 584 224 228 4 795.89 1.95 585 224 229 5 789.93 4.91 586 225 228 3 814.14 0.91 587 225 229 4 804.50 2.35 588 226 229 3 813.10 0.40 589 226 230 4 804.50 2.35 590 227 230 3 810.94 0.69 591 227 231 4 808.98 0.04 592 228 231 3 815.37 2.91 593 228 232 4 809.24 0.16 594 229 232 3 811.53 0.98 595 229 233 4 808.98 0.04 596 230 233 3 802.12 3.39 597 230 234 4 776.99 7.02 598 232 235 3 800.19 4.53 599 233 236 3 822.30 3.12