BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 9 127 119 46.41 0.75 2 10 127 118 47.70 1.05 3 10 128 119 47.94 0.94 4 11 14 3 843.08 1.10 5 11 125 115 47.95 0.94 6 11 126 116 48.11 1.00 7 11 127 117 48.11 1.00 8 12 23 11 48.22 0.99 9 12 128 117 48.24 0.98 10 12 129 118 48.23 0.97 11 13 21 8 334.02 141.66 12 13 23 10 48.23 0.99 13 13 24 11 48.24 0.98 14 13 124 112 48.22 0.98 15 13 131 119 48.22 0.97 16 14 21 7 527.59 45.00 17 15 20 5 527.63 45.01 18 15 21 6 527.61 44.99 19 16 19 3 796.08 5.38 20 16 20 4 531.87 46.06 21 20 25 5 796.88 3.07 22 21 24 3 824.59 5.67 23 21 25 4 796.89 3.08 24 22 25 3 815.99 2.81 25 22 26 4 796.90 3.08 26 23 26 3 810.99 3.77 27 23 27 4 810.80 3.87 28 23 131 109 361.87 1.24 29 24 27 3 819.68 3.00 30 24 28 4 810.80 3.87 31 24 124 101 361.84 1.23 32 24 131 108 361.91 1.21 33 24 154 132 360.67 1.02 34 25 28 3 814.81 5.88 35 25 29 4 802.76 7.88 36 26 29 3 817.97 3.87 37 26 30 4 802.79 7.90 38 27 30 3 817.52 0.54 39 27 31 4 802.79 7.90 40 27 131 105 370.25 0.21 41 27 132 106 370.25 0.21 42 27 133 107 370.24 0.22 43 27 153 128 370.20 0.20 44 28 31 3 818.39 4.08 45 28 32 4 813.94 2.34 46 28 152 126 370.21 0.20 47 28 153 127 370.22 0.20 48 29 32 3 817.90 4.33 49 29 33 4 813.94 2.34 50 30 33 3 817.38 4.06 51 30 34 4 813.94 2.34 52 31 34 3 813.94 2.35 53 31 79 48 370.51 0.18 54 31 80 49 370.52 0.18 55 31 133 103 370.53 0.18 56 31 134 104 370.51 0.18 57 31 135 105 370.50 0.18 58 31 153 124 370.51 0.18 59 31 166 137 347.53 1.76 60 32 166 136 347.50 1.74 61 32 167 137 347.48 1.75 62 32 169 139 334.08 2.08 63 33 167 136 347.53 1.76 64 34 80 46 372.89 0.48 65 34 166 134 348.25 1.99 66 34 167 135 348.08 2.00 67 35 51 16 395.60 0.58 68 35 53 18 395.56 0.57 69 35 66 31 395.22 0.76 70 35 79 44 375.71 0.49 71 35 166 133 348.49 1.91 72 35 167 134 348.35 1.97 73 36 50 14 395.72 0.53 74 36 164 130 348.85 1.83 75 36 165 131 348.75 1.83 76 36 166 132 348.53 1.90 77 36 167 133 348.49 1.91 78 36 168 134 348.48 1.91 79 36 200 166 250.57 1.10 80 37 49 12 395.81 0.48 81 37 50 13 395.72 0.53 82 37 77 40 395.08 0.80 83 37 78 41 394.12 0.93 84 37 79 42 393.42 1.16 85 37 137 101 394.61 1.03 86 37 164 129 348.85 1.83 87 37 165 130 348.76 1.83 88 38 48 10 395.85 0.48 89 38 49 11 395.86 0.48 90 38 137 100 395.04 0.82 91 38 163 127 349.07 1.84 92 38 164 128 348.86 1.84 93 38 193 157 252.67 1.06 94 38 196 160 252.47 0.99 95 38 197 161 252.55 1.04 96 39 47 8 396.12 0.57 97 39 48 9 395.85 0.49 98 39 137 99 395.50 0.59 99 39 148 111 395.81 0.49 100 39 162 125 358.68 2.70 101 39 163 126 350.44 1.26 102 40 46 6 528.25 45.19 103 40 47 7 416.22 10.52 104 40 148 110 396.03 0.55 105 40 161 123 371.73 0.10 106 40 162 124 361.53 1.31 107 40 176 138 318.53 3.93 108 40 180 142 294.44 1.58 109 40 189 151 252.89 1.12 110 40 192 154 252.77 1.07 111 40 193 155 252.68 1.06 112 41 45 4 771.65 1.42 113 41 46 5 528.45 45.08 114 41 147 107 399.63 2.08 115 41 147 108 396.26 0.48 116 41 148 109 396.06 0.57 117 41 160 121 372.75 0.24 118 41 161 122 371.95 0.00 119 41 180 141 294.52 1.54 120 42 45 3 810.83 3.33 121 42 46 4 606.71 81.06 122 42 184 144 259.30 0.40 123 42 185 145 258.80 0.15 124 42 186 146 258.17 0.45 125 42 189 149 254.41 1.06 126 43 147 105 436.89 0.38 127 43 182 141 280.96 5.13 128 43 183 142 277.81 6.30 129 43 184 143 275.88 6.62 130 44 147 104 437.09 0.37 131 44 185 143 261.38 0.65 132 44 186 144 258.81 0.17 133 45 147 103 437.26 0.28 134 45 186 143 257.96 0.53 135 45 215 172 249.95 1.12 136 45 216 173 249.82 1.18 137 46 139 94 437.00 0.35 138 46 147 102 437.34 0.25 139 46 147 103 437.30 0.27 140 46 148 104 437.00 0.35 141 46 186 142 257.95 0.53 142 46 214 170 250.02 1.09 143 46 215 171 249.93 1.14 144 47 186 141 257.78 0.62 145 47 189 144 252.93 1.11 146 47 190 145 252.90 1.12 147 47 193 148 252.78 1.06 148 47 212 167 250.25 1.08 149 47 213 168 250.12 1.10 150 47 214 169 249.83 1.18 151 48 67 19 437.39 0.26 152 48 75 27 437.44 0.27 153 48 76 28 437.40 0.26 154 48 77 29 437.14 0.35 155 48 137 90 437.28 0.29 156 48 139 92 437.42 0.27 157 48 148 102 437.41 0.27 158 48 212 166 250.14 1.09 159 48 213 167 250.03 1.10 160 49 197 150 252.60 1.03 161 49 211 164 250.18 1.11 162 49 212 165 249.92 1.15 163 50 66 16 437.48 0.25 164 50 67 17 437.49 0.25 165 50 77 27 437.42 0.27 166 50 197 149 252.56 1.04 167 50 210 162 250.34 1.10 168 50 211 163 250.17 1.04 169 51 66 15 437.53 0.24 170 51 202 152 250.97 1.03 171 51 209 160 250.41 1.08 172 51 210 161 250.34 1.10 173 52 61 9 437.57 0.26 174 52 66 14 437.54 0.24 175 52 209 158 250.46 1.07 176 52 209 159 250.41 1.08 177 52 210 160 250.40 1.08 178 52 211 161 250.40 1.08 179 53 203 151 250.82 0.94 180 56 59 3 811.84 1.48 181 60 63 3 836.04 5.53 182 61 64 3 841.11 2.99 183 61 209 149 250.49 1.05 184 65 211 148 250.30 1.08 185 65 223 175 111.74 0.51 186 66 77 11 559.33 27.21 187 66 78 12 549.28 32.23 188 66 85 19 548.93 32.05 189 66 211 147 250.19 1.12 190 67 76 9 570.07 22.63 191 67 77 10 566.66 23.55 192 67 85 18 569.26 22.23 193 67 211 146 250.21 1.13 194 67 213 148 249.94 1.16 195 67 221 171 116.86 0.51 196 67 223 173 109.94 1.41 197 68 75 7 575.06 20.21 198 68 76 8 575.01 20.18 199 68 85 17 572.84 20.45 200 68 89 21 573.59 19.68 201 68 93 25 574.10 19.42 202 68 221 170 116.86 0.51 203 69 72 3 846.33 1.66 204 69 74 5 577.55 21.12 205 69 75 6 575.63 20.36 206 69 93 24 574.11 19.41 207 69 221 169 116.85 0.51 208 70 74 4 740.19 0.92 209 70 75 5 577.47 21.08 210 70 76 6 575.98 20.34 211 70 89 19 574.43 19.59 212 70 92 22 574.09 19.41 213 70 93 23 574.09 19.41 214 70 112 43 572.98 18.84 215 70 138 69 537.42 1.05 216 71 74 3 785.82 2.77 217 71 96 25 595.61 8.65 218 71 106 36 599.06 6.73 219 71 107 37 598.08 7.18 220 71 112 42 595.19 8.53 221 72 106 35 599.37 6.58 222 72 219 164 117.40 0.65 223 72 220 165 117.39 0.66 224 72 221 166 117.27 0.62 225 73 140 68 593.02 3.38 226 73 141 69 593.02 3.37 227 73 215 144 249.97 1.17 228 73 219 163 117.41 0.65 229 73 220 164 117.28 0.61 230 74 107 34 599.34 6.53 231 74 109 36 599.11 6.42 232 74 112 39 599.10 6.42 233 74 138 65 593.22 3.46 234 74 139 66 593.05 3.38 235 74 140 67 593.02 3.38 236 74 215 143 249.93 1.14 237 75 138 64 593.24 3.46 238 75 139 65 593.12 3.39 239 75 215 142 249.75 1.22 240 75 221 163 116.82 0.49 241 76 85 9 600.74 7.15 242 76 137 62 593.25 3.46 243 76 138 63 593.25 3.46 244 77 85 8 600.75 7.15 245 77 136 60 593.37 3.50 246 77 137 61 593.35 3.49 247 78 82 4 811.02 2.58 248 78 85 7 600.76 7.14 249 78 134 57 598.10 5.84 250 78 135 58 593.88 3.74 251 78 136 59 593.38 3.49 252 78 165 89 348.72 1.82 253 79 134 56 598.11 5.84 254 79 135 57 596.62 5.10 255 79 165 88 348.51 1.90 256 79 166 89 347.81 1.81 257 80 133 54 598.24 5.91 258 80 134 55 598.11 5.83 259 81 84 3 821.90 3.08 260 81 85 4 806.50 1.13 261 81 130 50 598.44 5.95 262 81 133 53 598.26 5.90 263 81 134 54 598.26 5.90 264 82 85 3 818.23 2.26 265 82 86 4 796.01 2.08 266 83 86 3 817.42 2.64 267 83 87 4 802.20 4.97 268 84 87 3 827.32 0.36 269 84 88 4 802.03 5.03 270 84 130 47 598.53 5.91 271 84 132 49 598.46 5.91 272 84 133 50 598.43 5.87 273 84 134 51 598.42 5.85 274 85 88 3 808.76 1.67 275 85 89 4 802.04 5.03 276 85 134 50 598.25 5.77 277 85 136 52 593.95 3.60 278 86 89 3 825.37 1.31 279 86 90 4 808.77 1.68 280 87 90 3 820.04 3.96 281 87 91 4 820.04 3.96 282 87 132 46 598.63 5.83 283 88 91 3 820.05 3.97 284 88 92 4 796.75 0.71 285 88 116 29 599.56 6.22 286 88 119 32 599.49 6.19 287 88 120 33 599.48 6.20 288 88 132 45 598.62 5.82 289 88 134 47 598.40 5.70 290 88 136 49 593.99 3.46 291 89 92 3 805.12 3.47 292 89 93 4 795.89 1.26 293 90 93 3 825.96 5.12 294 90 94 4 793.57 2.43 295 91 94 3 819.21 1.75 296 91 95 4 793.57 2.42 297 91 119 29 599.64 6.17 298 92 95 3 810.76 2.93 299 92 96 4 797.58 0.41 300 92 112 21 599.86 6.19 301 92 116 25 599.69 6.16 302 93 96 3 816.35 0.14 303 93 97 4 804.08 2.89 304 94 97 3 807.16 4.46 305 94 98 4 804.03 2.91 306 95 98 3 803.07 3.39 307 95 99 4 781.77 0.88 308 95 112 18 599.91 6.20 309 95 115 21 599.72 6.16 310 96 100 4 762.49 7.26 311 96 101 5 759.78 8.61 312 96 107 12 600.03 6.20 313 96 112 17 599.88 6.20 314 97 100 3 796.90 0.30 315 97 101 4 756.09 10.46 316 97 102 5 754.31 11.34 317 97 106 10 600.66 6.13 318 98 102 4 754.31 11.34 319 98 102 5 775.88 0.56 320 100 102 3 790.12 2.18 321 100 103 4 762.91 6.92 322 100 105 6 614.56 11.92 323 100 106 7 600.66 6.15 324 100 107 8 600.62 6.12 325 100 108 9 600.66 6.14 326 100 111 12 600.58 6.11 327 101 104 4 610.57 11.08 328 101 105 5 600.70 6.14 329 101 106 6 600.67 6.15 330 107 111 4 785.43 1.24 331 107 112 5 780.56 0.82 332 108 111 3 824.26 2.07 333 108 112 4 793.88 0.18 334 108 142 34 641.02 20.64 335 108 145 37 641.07 20.69 336 109 112 3 827.21 0.43 337 109 113 4 793.89 0.18 338 109 138 29 641.24 20.53 339 109 140 31 641.17 20.56 340 109 141 32 641.19 20.58 341 109 147 39 640.94 20.67 342 109 149 41 640.85 20.66 343 110 113 3 793.90 0.18 344 110 114 4 799.39 2.93 345 110 145 35 641.18 20.69 346 110 149 40 640.92 20.69 347 111 114 3 802.52 4.49 348 111 115 4 799.50 2.89 349 112 115 3 799.77 2.75 350 112 116 4 799.50 2.89 351 113 116 3 804.83 4.32 352 113 117 4 800.72 2.27 353 113 138 25 641.27 20.54 354 113 149 37 641.00 20.70 355 113 151 39 640.70 20.73 356 113 157 45 472.23 5.66 357 114 117 3 795.21 0.48 358 114 118 4 794.27 0.95 359 114 157 44 472.55 5.53 360 115 118 3 816.83 7.73 361 115 119 4 796.41 2.00 362 116 119 3 818.71 6.79 363 116 120 4 797.57 1.46 364 116 136 20 641.94 20.27 365 116 138 22 641.43 20.57 366 117 120 3 822.49 3.25 367 117 121 4 803.05 1.08 368 117 151 35 640.95 20.71 369 117 155 39 515.67 0.21 370 117 157 41 472.55 5.53 371 118 121 3 824.58 2.21 372 118 122 4 807.83 1.30 373 119 122 3 827.44 2.44 374 119 123 4 812.79 1.18 375 119 132 13 646.47 18.14 376 120 123 3 812.79 1.18 377 120 124 4 813.55 0.80 378 120 132 12 646.45 18.14 379 120 134 14 646.41 18.13 380 120 151 32 641.11 20.72 381 120 152 33 641.03 20.72 382 120 153 34 640.56 20.80 383 120 155 36 611.27 27.64 384 121 124 3 812.41 0.24 385 121 125 4 806.88 2.54 386 121 154 34 640.18 20.62 387 121 155 35 626.96 15.50 388 122 125 3 819.57 8.89 389 122 126 4 786.63 0.83 390 123 129 6 646.70 18.16 391 123 130 7 646.70 18.16 392 123 131 8 646.59 18.15 393 123 132 9 646.59 18.15 394 124 131 7 646.65 18.14 395 124 153 30 641.05 20.71 396 124 154 31 640.87 20.71 397 126 129 3 844.56 2.89 398 131 153 23 641.03 20.73 399 132 153 22 640.88 20.79 400 134 153 20 640.89 20.93 401 135 151 17 641.33 21.00 402 135 152 18 640.92 20.93 403 135 153 19 640.84 20.98 404 135 165 31 348.51 1.90 405 136 150 15 641.45 21.00 406 136 151 16 641.34 21.03 407 136 165 30 348.56 1.91 408 137 148 12 641.65 21.02 409 137 149 13 641.54 21.04 410 137 150 14 641.35 21.07 411 137 163 27 348.88 1.83 412 137 165 29 348.73 1.83 413 138 148 11 641.62 21.05 414 138 149 12 641.49 21.07 415 138 151 14 641.37 21.13 416 139 147 9 641.67 21.05 417 139 148 10 641.54 21.09 418 139 215 77 249.76 1.21 419 140 147 7 641.69 21.05 420 140 147 8 641.62 21.08 421 140 215 76 249.93 1.14 422 141 146 5 641.70 21.07 423 141 147 6 641.68 21.07 424 141 215 75 249.95 1.12 425 141 220 95 117.30 0.61 426 142 145 3 819.46 1.90 427 142 146 4 641.77 21.07 428 147 215 69 249.94 1.13 429 147 216 70 249.93 1.13 430 148 159 11 467.03 6.74 431 148 160 12 462.57 6.33 432 148 161 13 448.11 0.84 433 148 162 14 409.18 18.61 434 149 157 8 471.92 5.68 435 149 158 9 471.71 5.78 436 149 159 10 467.04 6.71 437 150 157 7 471.91 5.68 438 150 158 8 471.86 5.71 439 150 160 10 467.48 6.71 440 150 165 15 348.55 1.91 441 151 156 5 482.45 0.58 442 151 157 6 472.03 5.74 443 152 155 3 799.02 0.01 444 152 156 4 623.56 14.64 445 152 158 6 537.57 28.36 446 160 163 3 845.15 3.00 447 161 180 19 281.73 4.78 448 162 176 14 318.28 3.90 449 162 180 18 281.70 4.77 450 163 173 10 319.37 3.60 451 163 176 13 317.79 4.15 452 164 169 5 347.47 1.74 453 164 172 8 319.83 3.36 454 164 173 9 318.50 3.97 455 164 176 12 318.20 3.79 456 165 169 4 479.84 70.81 457 166 169 3 800.03 0.41 458 168 171 3 820.44 5.87 459 168 172 4 790.01 1.92 460 169 172 3 795.76 2.54 461 169 173 4 790.02 1.92 462 170 173 3 801.40 0.92 463 170 174 4 792.88 3.35 464 171 174 3 801.41 0.92 465 171 175 4 797.27 1.15 466 172 175 3 799.73 0.66 467 172 176 4 793.16 3.96 468 172 196 24 251.44 0.94 469 173 176 3 804.85 0.81 470 173 177 4 793.17 3.96 471 174 177 3 803.80 1.33 472 174 178 4 803.78 1.34 473 175 178 3 811.96 0.90 474 175 179 4 803.78 1.34 475 175 195 20 252.58 1.08 476 175 196 21 251.65 0.85 477 175 199 24 251.47 0.77 478 176 179 3 808.49 2.63 479 176 180 4 787.51 6.79 480 176 192 16 252.63 1.09 481 176 196 20 251.71 1.26 482 179 192 13 252.63 1.09 483 180 184 4 798.23 1.00 484 181 184 3 834.83 2.43 485 184 188 4 802.91 0.37 486 184 192 8 741.81 10.52 487 185 188 3 816.74 2.12 488 185 189 4 802.92 0.36 489 186 189 3 808.08 2.21 490 186 190 4 802.91 0.36 491 186 214 28 250.25 1.07 492 186 216 30 249.94 1.14 493 187 190 3 802.93 0.36 494 187 191 4 802.89 0.35 495 187 232 60 80.14 2.00 496 188 191 3 806.44 2.14 497 188 192 4 802.89 0.34 498 189 192 3 802.89 0.34 499 189 193 4 798.66 1.86 500 190 193 3 799.71 1.34 501 190 194 4 798.83 1.94 502 190 212 22 250.28 1.07 503 190 214 24 250.10 1.08 504 190 224 49 113.75 0.01 505 190 232 57 78.78 1.45 506 191 194 3 809.16 3.17 507 191 195 4 799.07 2.06 508 191 235 59 67.73 0.41 509 191 236 60 53.27 2.08 510 192 195 3 818.64 1.58 511 192 196 4 799.09 2.06 512 193 196 3 805.81 3.24 513 193 197 4 799.86 1.67 514 193 212 19 250.21 1.10 515 194 197 3 808.09 2.92 516 194 198 4 807.84 2.23 517 194 206 11 250.59 1.07 518 194 210 16 250.38 1.07 519 194 212 18 250.27 1.06 520 194 229 50 84.81 0.03 521 194 232 53 69.72 0.45 522 194 233 54 69.31 0.42 523 195 198 3 819.89 2.98 524 195 199 4 808.68 2.62 525 195 236 56 21.82 0.65 526 196 199 3 821.24 2.05 527 196 200 4 819.63 2.85 528 197 200 3 819.62 2.85 529 197 202 4 802.91 0.24 530 197 206 8 250.45 1.06 531 197 210 13 250.32 1.09 532 197 212 15 250.12 1.10 533 198 202 3 802.68 0.35 534 198 206 7 250.41 1.06 535 198 236 53 24.05 1.25 536 202 206 4 769.65 12.16 537 202 210 9 250.33 1.09 538 205 209 5 766.36 2.77 539 206 209 4 766.37 2.77 540 206 210 5 764.78 1.98 541 206 233 43 69.53 0.52 542 207 209 3 800.63 0.96 543 207 210 4 764.79 1.98 544 207 230 39 84.78 0.03 545 207 233 42 69.55 0.53 546 208 226 34 85.62 0.36 547 208 229 37 84.82 0.02 548 208 230 38 84.81 0.02 549 208 233 41 69.69 0.58 550 209 226 33 87.53 1.31 551 210 229 34 84.81 0.02 552 211 223 27 109.95 1.41 553 211 229 33 84.80 0.03 554 212 223 26 109.97 1.39 555 212 224 27 109.25 1.25 556 212 229 32 84.80 0.03 557 213 221 23 117.00 0.58 558 213 222 24 116.45 0.67 559 213 223 25 109.94 1.41 560 213 224 26 109.92 1.41 561 214 221 22 116.99 0.58 562 214 222 23 116.81 0.51 563 214 224 25 114.08 0.23 564 215 220 20 117.17 0.56 565 215 221 21 116.99 0.58 566 216 220 19 117.20 0.55 567 217 220 18 117.41 0.65 568 217 217 14 117.73 0.64 569 217 218 15 117.66 0.65 570 217 219 16 117.59 0.66 571 217 220 17 117.42 0.65 572 217 217 3 820.07 3.04 573 217 217 4 795.51 4.04 574 217 218 14 117.70 0.64 575 217 219 15 117.70 0.64 576 217 222 18 117.45 0.66 577 217 217 3 813.97 4.19 578 217 217 12 117.90 0.58 579 217 218 13 117.73 0.65 580 217 219 12 117.94 0.56 581 217 222 15 117.58 0.65 582 217 217 8 118.09 0.55 583 217 218 10 117.97 0.56 584 217 219 11 117.97 0.56 585 217 217 3 842.36 2.19 586 222 228 6 787.25 1.54 587 224 228 4 778.81 5.76 588 224 229 5 767.65 3.14 589 225 228 3 820.47 0.68 590 225 229 4 794.13 1.91 591 226 229 3 801.09 0.40 592 226 230 4 793.58 3.67 593 227 230 3 812.35 4.88 594 227 231 4 795.51 2.71 595 228 231 3 818.15 6.00 596 228 232 4 795.53 2.70 597 229 232 3 812.38 3.12 598 229 233 4 795.51 2.70 599 230 233 3 799.50 0.47 600 230 234 4 793.71 3.19 601 232 235 3 798.74 1.21 602 233 236 3 810.46 4.08