BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 17 63 43 56.59 2.01 2 17 64 44 67.34 2.68 3 18 61 40 70.28 0.19 4 18 62 41 70.29 0.19 5 18 63 42 67.91 0.99 6 18 64 43 76.97 1.01 7 18 96 75 74.42 1.82 8 18 97 76 70.67 0.04 9 18 246 216 70.56 0.09 10 19 64 42 78.14 1.28 11 19 66 44 78.72 1.14 12 19 94 72 78.36 1.27 13 19 95 73 77.86 1.23 14 19 96 74 76.46 0.87 15 19 97 75 75.67 1.26 16 20 63 40 78.17 1.28 17 20 64 41 78.23 1.31 18 20 65 42 78.22 1.32 19 20 66 43 78.72 1.14 20 20 97 74 76.50 0.86 21 20 99 76 77.67 1.19 22 21 66 42 79.20 1.06 23 21 68 44 79.59 0.99 24 21 86 62 78.22 1.31 25 21 89 65 78.43 1.28 26 21 95 71 78.39 1.28 27 21 97 73 78.16 1.28 28 21 98 74 77.87 1.23 29 21 99 75 78.16 1.28 30 22 29 7 192.94 0.35 31 22 66 41 79.73 0.98 32 22 67 42 79.72 0.98 33 22 68 43 79.70 0.99 34 22 86 61 78.44 1.25 35 22 99 74 78.17 1.28 36 22 100 75 78.17 1.28 37 23 29 6 192.95 0.35 38 23 67 41 79.75 0.96 39 23 68 42 79.75 0.97 40 23 71 45 79.62 1.01 41 23 72 46 79.61 1.01 42 23 73 47 79.21 1.06 43 23 86 60 79.67 1.00 44 23 100 74 78.31 1.30 45 23 101 75 78.39 1.28 46 24 29 5 192.96 0.35 47 24 30 6 192.63 0.21 48 24 31 7 191.47 0.39 49 24 44 17 79.71 0.98 50 24 67 40 80.02 0.96 51 25 28 3 691.46 0.33 52 25 29 4 626.07 2.22 53 25 73 45 79.89 0.93 54 25 101 73 79.36 0.88 55 25 190 153 70.49 0.01 56 25 212 175 70.67 0.06 57 26 30 4 626.61 1.95 58 26 215 177 70.56 0.01 59 27 30 3 700.07 1.00 60 27 46 16 79.75 0.96 61 27 47 17 79.75 0.96 62 27 215 176 70.66 0.05 63 27 270 231 70.70 0.06 64 27 271 232 70.70 0.06 65 28 46 15 79.76 0.96 66 28 47 16 79.75 0.96 67 28 48 17 79.77 0.96 68 28 49 18 80.12 1.00 69 28 101 70 79.88 0.97 70 28 119 88 79.79 0.96 71 28 211 171 70.72 0.05 72 28 212 172 70.68 0.06 73 28 236 196 79.97 0.97 74 28 271 231 70.71 0.06 75 29 44 12 80.14 0.99 76 29 45 13 80.02 0.96 77 29 48 16 80.11 1.01 78 29 49 17 80.14 1.00 79 29 67 35 80.31 1.02 80 30 44 11 80.16 1.01 81 30 45 12 80.14 1.00 82 30 46 13 80.14 1.00 83 30 270 228 70.72 0.06 84 31 43 9 80.19 1.00 85 31 44 10 80.17 1.01 86 32 38 3 592.84 8.62 87 32 42 7 80.42 1.02 88 32 43 8 80.24 1.00 89 32 44 9 80.23 1.01 90 32 45 10 80.24 1.00 91 38 42 4 616.68 1.02 92 38 43 5 395.65 63.32 93 39 42 3 693.02 2.75 94 39 43 4 632.87 8.43 95 40 43 3 692.59 2.96 96 41 44 3 679.31 1.33 97 41 63 22 114.03 1.78 98 41 64 23 110.62 0.07 99 41 65 24 108.75 0.97 100 44 49 5 607.00 0.52 101 44 65 21 121.12 0.53 102 44 67 23 121.24 0.48 103 45 49 4 607.38 0.34 104 45 270 216 108.59 0.93 105 46 49 3 641.89 3.43 106 46 50 4 627.94 3.56 107 46 270 215 108.59 0.92 108 47 50 3 666.20 4.96 109 47 51 4 634.43 0.33 110 47 118 71 119.17 0.51 111 47 119 72 115.39 0.83 112 47 263 207 109.67 0.59 113 47 266 210 108.67 0.95 114 47 267 211 108.62 0.94 115 47 270 214 108.59 0.93 116 47 271 215 108.59 0.93 117 48 51 3 641.92 3.41 118 48 52 4 634.43 0.33 119 48 99 51 121.02 0.49 120 48 100 52 121.02 0.49 121 48 101 53 120.83 0.41 122 48 118 70 120.08 0.11 123 49 52 3 650.26 1.45 124 49 53 4 640.59 2.19 125 49 65 16 123.84 0.58 126 50 53 3 649.96 0.63 127 50 54 4 646.34 0.69 128 50 266 207 111.59 0.52 129 50 269 210 108.64 0.95 130 50 270 211 108.62 0.94 131 51 54 3 652.46 2.38 132 51 55 4 640.81 2.08 133 51 99 48 122.26 0.28 134 51 118 67 121.23 0.48 135 51 238 178 121.81 0.49 136 51 257 197 121.56 0.38 137 51 263 203 120.79 0.38 138 51 266 206 115.67 0.44 139 52 55 3 650.83 1.55 140 52 56 4 633.69 7.00 141 52 63 11 183.56 0.96 142 52 65 13 125.62 0.28 143 52 99 47 122.27 0.28 144 53 56 3 650.84 1.55 145 53 57 4 648.06 0.07 146 53 63 10 354.07 84.36 147 54 57 3 648.65 0.34 148 54 58 4 648.09 0.05 149 54 257 194 121.89 0.54 150 54 262 199 121.89 0.54 151 55 58 3 648.09 0.05 152 55 59 4 648.08 0.05 153 55 61 6 610.10 0.40 154 55 97 42 124.01 0.58 155 55 99 44 123.86 0.56 156 55 250 186 123.34 0.30 157 55 253 189 121.92 0.53 158 55 257 193 121.92 0.53 159 56 59 3 648.08 0.04 160 56 60 4 613.37 1.19 161 56 61 5 600.40 1.65 162 56 63 7 552.02 2.27 163 57 60 3 648.07 0.04 164 58 253 186 123.86 0.56 165 58 256 189 123.64 0.48 166 59 249 181 123.98 0.54 167 59 250 182 123.98 0.55 168 59 253 185 123.91 0.54 169 68 71 3 641.68 5.24 170 68 72 4 628.49 8.35 171 68 85 17 173.34 1.48 172 68 86 18 166.88 1.14 173 68 89 21 168.06 1.17 174 70 84 14 173.94 1.68 175 70 85 15 173.87 1.69 176 72 84 12 174.79 1.86 177 72 85 13 174.04 1.65 178 72 86 14 174.10 1.68 179 73 85 12 174.25 1.60 180 73 86 13 174.16 1.65 181 73 87 14 174.10 1.68 182 73 101 28 173.34 1.42 183 73 102 29 173.33 1.41 184 73 108 35 173.99 1.66 185 74 83 9 175.21 1.73 186 74 84 10 175.16 1.72 187 74 85 11 174.41 1.68 188 74 87 13 173.94 1.70 189 74 108 34 173.93 1.69 190 75 83 8 175.23 1.73 191 75 84 9 175.21 1.71 192 76 81 5 182.57 4.51 193 76 82 6 176.12 1.31 194 76 83 7 175.24 1.73 195 77 80 3 668.66 11.21 196 77 81 4 198.47 5.50 197 77 82 5 176.22 1.34 198 82 92 10 384.62 66.77 199 83 87 4 632.85 7.03 200 83 88 5 576.56 0.97 201 83 92 9 384.66 66.75 202 83 107 24 242.75 1.47 203 83 108 25 242.31 1.41 204 83 111 28 242.71 1.52 205 84 87 3 693.47 1.20 206 84 88 4 618.69 0.05 207 85 88 3 663.79 4.17 208 85 89 4 638.74 4.55 209 86 89 3 647.05 4.49 210 86 90 4 648.27 11.94 211 86 98 12 242.53 1.54 212 86 100 14 242.52 1.54 213 86 102 16 242.24 1.46 214 87 90 3 659.29 6.43 215 87 91 4 648.27 11.94 216 87 102 15 242.16 1.42 217 87 108 21 246.51 2.48 218 87 111 24 246.76 2.55 219 87 112 25 246.50 2.50 220 88 91 3 648.29 11.93 221 88 92 4 618.00 9.75 222 89 93 4 606.75 4.13 223 89 94 5 410.20 78.91 224 89 95 6 385.20 66.41 225 90 93 3 635.93 0.81 226 90 95 5 385.20 66.42 227 90 96 6 385.19 66.42 228 90 97 7 385.13 66.44 229 90 98 8 246.97 2.61 230 90 112 22 246.97 2.62 231 90 239 140 246.99 2.62 232 90 240 141 246.99 2.62 233 90 241 142 246.99 2.62 234 91 111 20 246.97 2.62 235 91 112 21 246.97 2.62 236 95 241 137 313.36 1.53 237 96 240 135 313.32 1.53 238 96 241 136 313.37 1.53 239 96 246 141 313.36 1.53 240 97 238 132 313.30 1.54 241 97 239 133 313.31 1.53 242 97 240 134 313.33 1.53 243 97 250 144 313.32 1.53 244 98 102 4 666.31 6.31 245 98 238 131 313.31 1.54 246 98 239 132 313.34 1.53 247 99 238 130 313.33 1.53 248 100 118 18 313.33 1.54 249 101 212 102 304.00 2.73 250 101 236 126 313.35 1.53 251 102 108 6 624.29 3.99 252 102 112 10 406.26 44.52 253 102 114 12 313.90 1.49 254 102 236 125 313.46 1.51 255 102 237 126 313.54 1.53 256 102 239 128 313.91 1.48 257 103 108 5 624.30 3.99 258 103 114 11 313.94 1.48 259 103 158 46 313.56 1.52 260 103 209 97 313.47 1.50 261 103 234 122 313.75 1.48 262 103 235 123 313.50 1.51 263 103 236 124 313.50 1.50 264 104 109 5 624.30 4.00 265 104 114 10 313.94 1.48 266 104 115 11 313.95 1.48 267 104 117 13 313.85 1.46 268 104 234 121 313.76 1.48 269 104 235 122 313.75 1.48 270 104 236 123 313.80 1.50 271 104 237 124 313.89 1.48 272 105 108 3 649.38 1.68 273 105 109 4 633.01 0.35 274 105 158 44 313.82 1.50 275 106 109 3 648.01 2.36 276 106 110 4 633.01 0.35 277 107 110 3 664.49 8.43 278 108 111 3 656.85 10.30 279 108 112 4 633.00 0.27 280 108 114 6 588.97 8.04 281 109 112 3 652.50 10.02 282 109 114 5 579.32 4.33 283 110 242 123 419.86 0.24 284 111 242 122 420.54 0.58 285 112 239 118 413.79 1.63 286 112 240 119 419.79 0.24 287 112 242 121 420.81 0.72 288 113 239 117 419.78 0.24 289 113 240 118 419.84 0.24 290 113 242 120 420.94 0.73 291 113 243 121 421.00 0.70 292 114 237 114 402.91 0.35 293 114 238 115 419.79 0.23 294 114 239 116 419.84 0.24 295 115 237 113 418.10 0.60 296 115 238 114 420.89 0.76 297 115 251 127 419.17 0.48 298 115 254 130 417.50 0.35 299 116 237 112 430.23 0.09 300 116 251 126 421.31 0.70 301 116 254 129 420.44 0.48 302 116 255 130 421.17 0.58 303 117 235 109 419.77 0.24 304 117 236 110 444.28 2.56 305 117 254 128 421.59 0.78 306 118 236 109 450.76 3.00 307 118 237 110 450.78 3.01 308 118 238 111 450.79 3.01 309 118 254 127 421.59 0.78 310 118 263 136 412.39 1.22 311 119 211 83 392.98 0.89 312 119 235 107 450.76 3.00 313 119 236 108 450.77 3.00 314 119 267 139 407.74 0.90 315 119 271 143 392.44 0.62 316 120 211 82 407.48 0.80 317 120 234 105 450.77 3.00 318 120 235 106 450.77 3.00 319 121 209 79 442.69 7.04 320 121 210 80 429.34 9.67 321 121 211 81 407.52 0.78 322 121 233 103 450.78 3.00 323 121 234 104 450.78 3.00 324 121 235 105 450.79 3.01 325 121 236 106 450.86 3.02 326 122 209 78 447.84 4.48 327 122 210 79 441.89 7.38 328 122 230 99 438.78 0.41 329 122 232 101 450.75 3.00 330 122 233 102 450.78 3.00 331 123 159 27 450.44 3.31 332 123 162 30 450.91 3.06 333 123 163 31 450.92 3.06 334 123 207 75 450.02 3.44 335 123 208 76 448.56 4.17 336 123 209 77 448.25 4.28 337 123 230 98 449.99 3.04 338 123 231 99 450.69 3.04 339 123 232 100 450.78 3.01 340 123 234 102 450.87 3.02 341 124 207 74 450.42 3.25 342 124 208 75 450.17 3.37 343 124 209 76 448.80 4.05 344 124 210 77 448.74 4.04 345 124 213 80 448.34 4.21 346 124 226 93 437.70 0.59 347 124 230 97 450.68 3.05 348 124 231 98 450.78 3.01 349 125 139 5 450.91 3.06 350 125 140 6 450.91 3.06 351 125 143 9 450.89 3.06 352 125 205 71 450.71 3.09 353 125 206 72 450.73 3.10 354 125 207 73 450.77 3.10 355 125 213 79 448.75 4.02 356 125 226 92 438.38 0.65 357 125 227 93 442.36 1.11 358 125 231 97 450.85 3.02 359 126 194 59 450.75 3.12 360 126 197 62 450.74 3.11 361 126 205 70 450.73 3.08 362 126 206 71 450.74 3.10 363 126 208 73 450.88 3.07 364 126 213 78 450.34 3.23 365 126 216 81 447.65 3.64 366 126 224 89 437.70 0.59 367 126 225 90 438.81 0.44 368 126 226 91 439.06 0.35 369 127 197 61 450.75 3.12 370 127 205 69 450.76 3.10 371 127 224 88 438.80 0.45 372 127 225 89 439.24 0.23 373 127 227 91 450.50 2.90 374 128 197 60 450.77 3.11 375 128 198 61 450.71 3.09 376 128 201 64 450.73 3.14 377 128 202 65 450.69 3.16 378 128 223 86 438.38 0.66 379 128 224 87 438.80 0.45 380 128 225 88 446.85 1.17 381 138 142 4 636.91 3.94 382 138 143 5 636.91 3.94 383 138 227 89 450.82 3.00 384 138 228 90 450.84 3.00 385 139 142 3 637.10 4.03 386 139 143 4 636.92 3.94 387 140 143 3 649.74 2.46 388 140 144 4 636.93 3.94 389 140 170 30 452.46 3.38 390 140 171 31 452.45 3.38 391 140 174 34 452.30 3.46 392 140 231 91 450.99 2.96 393 141 144 3 657.04 3.42 394 141 145 4 636.92 3.95 395 141 170 29 452.46 3.37 396 142 145 3 664.54 3.38 397 142 146 4 641.88 1.44 398 143 146 3 653.01 3.67 399 143 149 6 547.03 3.12 400 143 151 8 451.60 3.26 401 143 174 31 452.48 3.38 402 143 205 62 451.58 3.27 403 143 207 64 451.56 3.25 404 144 151 7 465.89 10.21 405 144 170 26 454.38 4.29 406 144 173 29 454.38 4.29 407 144 174 30 452.41 3.39 408 145 173 28 465.37 8.90 409 149 202 53 488.31 14.46 410 149 203 54 488.28 14.45 411 149 204 55 488.29 14.44 412 149 205 56 488.36 14.39 413 150 177 27 507.65 12.14 414 150 185 35 507.47 12.16 415 150 203 53 488.41 14.42 416 150 204 54 488.38 14.41 417 150 205 55 488.38 14.40 418 151 174 23 507.74 12.13 419 151 177 26 507.68 12.16 420 151 178 27 507.59 12.16 421 151 185 34 507.55 12.13 422 151 204 53 488.40 14.41 423 151 205 54 488.39 14.41 424 151 207 56 488.35 14.41 425 152 178 26 507.62 12.16 426 152 185 33 507.56 12.14 427 152 204 52 488.51 14.43 428 152 205 53 488.46 14.40 429 152 206 54 488.36 14.42 430 152 207 55 488.36 14.42 431 153 159 6 508.66 11.86 432 153 160 7 507.94 12.20 433 153 163 10 507.93 12.19 434 153 178 25 507.71 12.13 435 153 186 33 507.56 12.14 436 153 187 34 507.54 12.13 437 153 206 53 488.43 14.41 438 153 207 54 488.06 14.58 439 154 160 6 550.99 11.64 440 154 186 32 507.56 12.14 441 154 187 33 507.50 12.14 442 154 188 34 507.49 12.16 443 154 189 35 507.48 12.16 444 154 190 36 507.12 12.27 445 154 206 52 488.50 14.42 446 155 160 5 620.86 1.65 447 155 186 31 507.56 12.18 448 155 187 32 507.53 12.17 449 155 188 33 507.51 12.17 450 156 160 4 630.51 0.45 451 157 160 3 674.34 0.29 452 157 161 4 630.52 0.45 453 158 161 3 644.72 6.65 454 158 162 4 635.82 2.20 455 158 209 51 487.32 15.00 456 159 162 3 664.41 3.20 457 159 163 4 655.03 1.50 458 159 207 48 488.45 14.44 459 159 209 50 487.04 15.10 460 160 163 3 683.26 1.21 461 160 164 4 644.88 3.58 462 160 175 15 508.17 12.14 463 161 164 3 655.01 1.49 464 161 165 4 644.88 3.58 465 162 165 3 675.95 1.08 466 162 166 4 622.93 2.12 467 162 171 9 508.37 12.18 468 162 231 69 470.70 6.92 469 162 232 70 470.71 6.92 470 163 166 3 653.57 2.17 471 163 171 8 508.36 12.18 472 163 174 11 508.29 12.19 473 163 175 12 508.18 12.14 474 163 178 15 508.18 12.14 475 163 207 44 488.42 14.45 476 163 231 68 470.69 6.92 477 164 175 11 508.19 12.15 478 166 171 5 564.00 14.45 479 166 172 6 508.36 12.18 480 168 171 3 668.72 1.96 481 168 172 4 625.40 0.93 482 169 172 3 675.45 1.41 483 169 173 4 632.71 4.09 484 170 173 3 667.00 5.63 485 170 174 4 648.31 3.71 486 171 174 3 654.67 4.84 487 171 175 4 625.94 0.67 488 171 231 60 470.25 6.71 489 172 175 3 645.49 3.21 490 172 176 4 625.92 0.55 491 173 176 3 662.16 5.12 492 173 177 4 625.92 0.55 493 174 177 3 645.74 9.30 494 174 178 4 636.94 4.90 495 174 207 33 487.29 14.96 496 175 178 3 675.10 6.41 497 175 179 4 636.94 4.90 498 175 186 11 510.32 11.93 499 176 179 3 680.30 3.81 500 176 180 4 659.03 6.82 501 177 180 3 675.36 1.33 502 177 181 4 625.93 0.60 503 177 185 8 523.24 5.48 504 178 181 3 660.73 5.97 505 178 185 7 510.33 11.93 506 178 186 8 510.33 11.93 507 178 207 29 488.06 14.58 508 179 186 7 510.70 11.73 509 181 184 3 703.96 1.42 510 181 185 4 624.49 0.10 511 184 196 12 502.47 7.52 512 185 196 11 502.45 7.52 513 185 204 19 488.49 14.41 514 186 196 10 512.17 2.66 515 187 192 5 602.97 0.98 516 187 193 6 542.33 12.35 517 187 196 9 536.76 14.96 518 187 204 17 488.63 14.39 519 187 206 19 488.46 14.40 520 188 192 4 626.40 0.40 521 189 192 3 638.69 3.23 522 189 193 4 627.61 0.98 523 190 193 3 651.42 10.88 524 190 194 4 652.84 1.90 525 190 206 16 488.60 14.38 526 190 208 18 488.41 14.40 527 190 212 22 477.69 12.12 528 191 194 3 660.32 5.63 529 191 195 4 652.86 1.91 530 192 195 3 652.87 1.91 531 192 196 4 652.86 1.91 532 193 196 3 653.39 2.17 533 193 197 4 652.86 1.91 534 193 206 13 488.53 14.38 535 194 197 3 660.82 1.54 536 194 198 4 643.15 6.76 537 194 206 12 488.61 14.38 538 194 216 22 447.12 0.19 539 194 224 30 442.91 2.28 540 195 198 3 664.29 3.84 541 195 199 4 643.05 6.77 542 196 199 3 672.78 0.41 543 196 200 4 643.04 6.77 544 196 204 8 488.64 14.39 545 197 200 3 672.96 8.20 546 197 204 7 488.64 14.39 547 197 206 9 488.57 14.36 548 197 224 27 442.90 2.27 549 198 216 18 451.89 2.20 550 198 219 21 449.56 1.04 551 198 220 22 448.33 0.45 552 198 221 23 442.88 2.28 553 198 223 25 442.88 2.25 554 198 224 26 443.00 2.23 555 200 203 3 625.97 0.04 556 200 204 4 511.38 10.65 557 207 231 24 665.42 0.26 558 208 212 4 648.29 2.42 559 208 213 5 643.70 0.55 560 209 212 3 667.65 3.46 561 209 213 4 655.91 1.39 562 209 236 27 675.81 0.89 563 210 213 3 660.72 3.54 564 210 214 4 656.01 1.34 565 210 226 16 659.45 0.38 566 210 230 20 666.63 0.35 567 211 214 3 665.57 1.11 568 211 215 4 665.59 1.09 569 211 236 25 722.04 6.94 570 211 271 60 660.34 0.34 571 212 215 3 671.32 1.47 572 212 216 4 666.02 0.88 573 212 236 24 770.24 30.34 574 213 216 3 672.78 0.76 575 213 217 4 671.34 1.48 576 213 226 13 666.62 0.33 577 214 217 3 674.50 0.10 578 214 218 4 674.50 0.10 579 214 267 53 679.31 3.38 580 214 271 57 668.50 0.66 581 214 272 58 668.50 0.66 582 215 218 3 683.03 4.16 583 215 219 4 674.22 0.04 584 215 271 56 668.40 0.71 585 216 219 3 674.22 0.03 586 216 224 8 675.88 0.80 587 217 224 7 676.85 1.28 588 217 225 8 728.19 4.70 589 217 226 9 849.38 1.56 590 217 277 60 668.40 0.71 591 218 271 53 807.09 12.45 592 218 272 54 836.76 2.37 593 218 275 57 668.61 0.61 594 218 276 58 668.53 0.73 595 218 277 59 668.32 0.75 596 219 222 3 688.04 0.87 597 219 276 57 669.11 0.57 598 219 277 58 670.70 0.22 599 220 276 56 680.11 2.92 600 220 277 57 685.13 0.39