BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 51 50 35.65 0.40 2 2 17 15 35.95 0.55 3 2 20 18 30.98 1.15 4 2 21 19 29.73 0.53 5 2 24 22 29.73 0.53 6 2 48 46 30.59 0.96 7 2 49 47 32.23 0.14 8 2 50 48 34.17 0.16 9 2 51 49 35.88 0.51 10 3 17 14 36.52 0.58 11 3 51 48 35.59 0.42 12 3 53 50 46.80 1.84 13 4 9 5 813.46 3.91 14 4 14 10 38.91 0.51 15 4 17 13 36.55 0.58 16 4 51 47 37.31 0.21 17 4 52 48 37.86 0.49 18 4 53 49 45.98 2.25 19 4 129 125 59.41 1.04 20 4 132 128 58.82 0.91 21 5 9 4 807.00 7.14 22 5 53 48 49.44 0.54 23 6 9 3 831.07 2.12 24 6 53 47 59.23 1.14 25 6 54 48 59.26 1.14 26 6 55 49 60.28 1.47 27 6 129 123 59.41 1.04 28 6 132 126 59.44 1.05 29 6 133 127 59.49 1.07 30 6 136 130 59.49 1.08 31 7 55 48 60.25 1.49 32 9 13 4 822.26 2.17 33 9 129 120 73.14 0.04 34 10 13 3 857.68 0.10 35 10 14 4 829.38 1.42 36 10 176 166 92.72 0.76 37 11 14 3 860.30 1.41 38 11 15 4 829.28 1.47 39 11 172 161 77.67 0.70 40 11 175 164 91.51 0.30 41 11 176 165 100.33 0.39 42 12 15 3 834.53 0.42 43 12 16 4 829.28 1.47 44 13 16 3 835.89 4.67 45 13 17 4 829.28 1.46 46 14 17 3 835.19 1.53 47 14 18 4 829.22 1.44 48 14 129 115 102.50 0.57 49 14 157 143 99.68 0.70 50 14 161 147 92.95 0.67 51 14 168 154 97.70 0.32 52 14 171 157 101.27 0.08 53 14 172 158 101.37 0.03 54 15 18 3 835.22 3.81 55 15 19 4 829.63 1.71 56 15 168 153 99.07 1.01 57 15 172 157 102.80 0.71 58 16 19 3 830.33 1.36 59 16 20 4 829.08 1.43 60 17 20 3 830.97 2.38 61 17 21 4 842.26 3.27 62 17 50 33 110.61 1.86 63 17 52 35 110.92 1.78 64 17 158 141 103.25 0.66 65 18 21 3 843.07 2.87 66 18 22 4 842.27 3.27 67 18 157 139 104.53 0.45 68 18 158 140 103.33 0.71 69 18 161 143 103.00 0.68 70 18 162 144 101.56 0.12 71 18 168 150 103.20 0.78 72 19 22 3 846.36 4.51 73 19 23 4 842.27 3.27 74 19 165 146 104.92 0.26 75 20 23 3 842.76 3.03 76 20 24 4 842.28 3.27 77 21 24 3 853.28 2.23 78 21 25 4 842.28 3.27 79 21 48 27 113.53 1.82 80 21 50 29 115.55 0.83 81 21 158 137 111.92 2.16 82 21 162 141 110.64 1.85 83 22 25 3 853.28 2.23 84 22 161 139 112.17 2.27 85 22 162 140 110.96 1.70 86 22 164 142 111.59 1.99 87 22 165 143 112.17 2.27 88 22 168 146 112.83 2.13 89 22 238 216 110.62 1.84 90 22 239 217 106.76 0.06 91 24 48 24 116.43 0.54 92 25 37 12 116.30 0.45 93 25 48 23 116.44 0.54 94 25 66 41 116.27 0.51 95 25 69 44 116.23 0.48 96 25 239 214 116.27 0.47 97 26 37 11 116.38 0.47 98 26 38 12 116.36 0.45 99 26 66 40 116.42 0.52 100 27 30 3 968.23 2.32 101 27 35 8 116.45 0.50 102 27 36 9 116.44 0.50 103 27 37 10 116.38 0.47 104 27 38 11 116.36 0.46 105 28 38 10 116.69 0.59 106 29 35 6 421.25 152.59 107 29 36 7 420.93 152.65 108 30 33 3 855.50 5.04 109 30 34 4 766.95 16.48 110 30 35 5 705.20 10.62 111 30 36 6 421.17 152.55 112 31 34 3 851.05 7.27 113 31 36 5 705.40 10.51 114 31 199 168 144.74 0.67 115 31 200 169 132.89 0.50 116 31 203 172 155.75 0.34 117 31 240 209 117.94 0.48 118 32 199 167 155.53 0.24 119 32 202 170 156.53 0.12 120 32 203 171 156.48 0.09 121 32 207 175 156.78 0.03 122 32 228 196 156.85 0.07 123 34 199 165 156.90 0.07 124 34 233 199 157.01 0.09 125 34 237 203 156.94 0.10 126 34 238 204 156.88 0.07 127 34 239 205 156.81 0.09 128 34 240 206 156.75 0.07 129 35 238 203 157.01 0.10 130 35 239 204 156.89 0.06 131 35 240 205 156.88 0.06 132 36 200 164 157.00 0.10 133 36 240 204 156.88 0.07 134 36 242 206 156.27 0.28 135 37 66 29 157.00 0.07 136 37 67 30 156.96 0.07 137 37 68 31 156.95 0.07 138 37 192 155 157.06 0.11 139 37 239 202 157.06 0.10 140 37 240 203 156.95 0.07 141 37 241 204 156.89 0.07 142 37 242 205 156.81 0.09 143 38 41 3 912.25 1.02 144 38 67 29 157.03 0.09 145 38 242 204 156.87 0.10 146 39 242 203 156.88 0.10 147 39 244 205 156.71 0.14 148 39 245 206 156.27 0.27 149 40 46 6 771.20 22.78 150 40 67 27 593.43 19.05 151 41 45 4 810.51 4.57 152 41 46 5 704.67 10.48 153 41 66 25 681.01 8.19 154 41 67 26 681.02 8.19 155 43 46 3 861.50 2.42 156 43 47 4 861.55 1.74 157 43 94 51 600.38 0.96 158 44 47 3 856.74 2.06 159 44 94 50 600.37 0.95 160 45 66 21 702.61 10.11 161 46 64 18 702.79 10.13 162 46 65 19 702.64 10.11 163 46 66 20 702.61 10.11 164 46 67 21 702.53 10.13 165 47 64 17 702.72 10.10 166 47 65 18 702.66 10.11 167 47 66 19 702.64 10.11 168 47 72 25 700.16 9.50 169 47 90 43 665.67 6.21 170 47 91 44 664.75 6.53 171 47 94 47 663.86 6.81 172 47 95 48 663.61 6.74 173 48 62 14 703.60 10.36 174 48 63 15 703.14 10.19 175 48 64 16 702.79 10.13 176 48 66 18 702.69 10.11 177 49 61 12 703.66 10.34 178 49 62 13 703.60 10.36 179 49 63 14 703.51 10.37 180 49 95 46 665.94 6.29 181 50 60 10 704.19 10.51 182 50 61 11 703.75 10.37 183 50 62 12 703.64 10.33 184 50 64 14 703.56 10.35 185 50 71 21 701.46 9.82 186 50 158 108 449.95 1.04 187 51 60 9 704.19 10.51 188 51 61 10 703.84 10.40 189 52 59 7 704.25 10.52 190 52 60 8 704.24 10.52 191 52 132 80 545.05 1.05 192 52 136 84 545.02 1.03 193 52 154 102 506.11 0.64 194 52 158 106 464.69 6.44 195 53 57 4 704.44 10.47 196 53 58 5 704.43 10.47 197 53 59 6 704.25 10.52 198 53 136 83 545.11 1.04 199 54 57 3 813.27 3.06 200 54 58 4 705.52 10.70 201 54 133 79 545.07 1.06 202 54 136 82 545.13 1.03 203 54 137 83 545.16 1.06 204 54 140 86 545.13 1.04 205 55 129 74 545.40 1.05 206 55 130 75 545.40 1.05 207 55 133 78 545.25 1.03 208 58 136 78 545.15 1.03 209 58 140 82 544.94 0.98 210 59 140 81 544.91 1.00 211 60 75 15 700.07 9.52 212 60 76 16 699.82 9.46 213 60 136 76 545.00 1.04 214 60 139 79 544.95 1.02 215 60 140 80 544.84 1.01 216 61 74 13 700.17 9.49 217 61 75 14 699.99 9.54 218 61 96 35 666.07 6.29 219 62 71 9 701.46 9.81 220 62 73 11 700.20 9.49 221 62 74 12 700.08 9.50 222 62 96 34 666.07 6.29 223 62 154 92 504.24 1.26 224 63 71 8 701.31 9.89 225 63 72 9 700.67 9.71 226 63 73 10 700.18 9.48 227 63 95 32 665.93 6.29 228 63 96 33 665.49 6.17 229 64 69 5 701.56 9.85 230 64 70 6 701.46 9.82 231 64 71 7 700.68 9.72 232 65 68 3 858.25 0.57 233 65 69 4 701.45 9.82 234 65 70 5 700.84 9.67 235 65 72 7 700.66 9.72 236 67 241 174 162.97 0.30 237 68 163 95 426.19 0.39 238 68 192 124 268.83 12.97 239 68 241 173 162.97 0.31 240 69 159 90 426.20 0.38 241 69 163 94 426.17 0.40 242 69 192 123 268.93 12.95 243 69 239 170 166.56 0.83 244 70 163 93 426.24 0.39 245 70 192 122 268.92 12.95 246 71 83 12 666.90 6.08 247 71 155 84 489.37 1.62 248 72 82 10 667.01 6.15 249 72 83 11 666.87 6.10 250 72 87 15 666.45 6.14 251 72 88 16 666.22 6.25 252 72 91 19 666.14 6.27 253 73 81 8 667.05 6.14 254 73 82 9 667.01 6.15 255 73 96 23 666.11 6.28 256 74 80 6 667.15 6.12 257 74 81 7 667.06 6.14 258 74 96 22 666.11 6.28 259 74 105 31 639.08 15.85 260 74 139 65 544.77 0.97 261 74 147 73 541.27 0.99 262 74 151 77 538.98 0.14 263 75 78 3 892.33 1.71 264 75 79 4 782.25 18.82 265 75 80 5 667.18 6.12 266 75 96 21 666.08 6.29 267 75 99 24 664.07 6.59 268 76 79 3 858.77 4.58 269 76 81 5 832.66 4.28 270 76 139 63 544.91 1.03 271 76 140 64 544.76 1.04 272 77 81 4 995.32 2.41 273 78 99 21 664.43 6.51 274 78 100 22 664.35 6.52 275 78 101 23 663.98 6.65 276 79 101 22 661.77 7.29 277 79 103 24 651.06 10.02 278 80 96 16 666.12 6.29 279 80 99 19 664.01 6.57 280 80 101 21 661.64 7.22 281 80 102 22 639.33 15.86 282 80 103 23 639.28 15.86 283 81 103 22 639.20 15.83 284 81 105 24 639.02 15.87 285 81 142 61 543.35 0.51 286 82 88 6 757.42 18.37 287 82 91 9 699.82 10.44 288 82 92 10 666.50 6.21 289 82 95 13 666.51 6.21 290 82 96 14 666.40 6.15 291 82 102 20 639.33 15.87 292 82 103 21 639.16 15.86 293 82 104 22 639.01 15.87 294 82 105 23 639.00 15.87 295 83 88 5 772.52 10.84 296 83 104 21 639.02 15.87 297 83 105 22 638.87 15.81 298 83 107 24 627.38 11.38 299 83 108 25 548.18 1.19 300 83 109 26 548.13 1.18 301 83 114 31 548.12 1.20 302 83 151 68 541.02 1.07 303 84 89 5 816.73 0.41 304 84 104 20 639.02 15.87 305 84 105 21 639.00 15.87 306 84 107 23 630.24 12.81 307 84 108 24 548.35 1.20 308 85 88 3 840.21 3.33 309 85 89 4 827.41 3.40 310 85 108 23 548.33 1.23 311 85 109 24 548.32 1.23 312 86 89 3 831.38 1.43 313 86 90 4 827.48 3.37 314 87 90 3 845.22 8.35 315 87 91 4 820.72 0.04 316 88 91 3 824.31 0.69 317 88 92 4 823.73 0.98 318 89 92 3 867.42 0.28 319 89 102 13 639.41 15.91 320 89 104 15 639.01 15.86 321 90 93 3 867.42 0.28 322 90 94 4 858.21 1.85 323 91 94 3 868.28 0.16 324 91 95 4 836.81 2.31 325 92 95 3 841.35 4.56 326 92 97 5 826.94 3.59 327 92 102 10 639.38 15.90 328 93 97 4 830.06 2.06 329 94 97 3 892.45 1.75 330 96 99 3 839.45 1.60 331 105 142 37 544.17 0.72 332 105 151 46 541.79 0.78 333 106 142 36 544.63 0.90 334 106 144 38 543.30 0.64 335 107 113 6 702.28 9.75 336 107 114 7 636.23 42.76 337 107 117 10 547.62 0.99 338 107 144 37 543.56 0.64 339 107 147 40 543.42 0.71 340 107 148 41 542.73 0.98 341 107 151 44 542.67 0.99 342 108 113 5 766.13 8.32 343 108 114 6 722.38 19.73 344 110 113 3 860.62 2.58 345 110 114 4 823.93 2.70 346 111 114 3 854.40 0.54 347 111 115 4 827.82 4.64 348 111 122 11 546.22 0.88 349 111 152 41 542.66 0.98 350 111 155 44 541.78 0.77 351 111 156 45 541.49 0.91 352 111 160 49 482.88 1.44 353 111 189 78 333.72 3.66 354 112 115 3 849.62 2.92 355 112 116 4 837.02 9.21 356 113 116 3 844.24 5.61 357 113 117 4 837.01 9.21 358 114 117 3 838.90 8.27 359 114 118 4 819.61 0.51 360 114 148 34 542.81 1.02 361 114 151 37 542.79 1.03 362 114 152 38 542.65 1.05 363 114 155 41 541.50 0.91 364 115 118 3 835.01 6.67 365 115 119 4 816.60 0.99 366 115 120 5 799.58 1.55 367 115 121 6 670.15 60.99 368 115 122 7 614.42 34.15 369 115 152 37 542.78 1.03 370 116 119 3 829.69 2.85 371 117 145 28 543.84 0.55 372 117 148 31 543.27 0.79 373 118 145 27 543.78 0.51 374 118 148 30 543.38 0.69 375 118 149 31 543.19 0.76 376 120 124 4 815.00 0.68 377 120 125 5 791.49 10.31 378 120 152 32 542.74 1.00 379 121 124 3 852.19 1.07 380 121 125 4 834.28 3.94 381 122 125 3 852.19 1.07 382 122 152 30 542.73 1.01 383 122 156 34 541.88 0.89 384 122 181 59 417.40 1.38 385 122 186 64 365.80 4.45 386 122 189 67 356.91 7.68 387 122 190 68 356.31 7.98 388 123 180 57 417.66 1.34 389 123 181 58 417.56 1.30 390 123 186 63 368.97 2.90 391 124 180 56 417.66 1.34 392 125 152 27 542.54 1.02 393 125 153 28 542.41 1.06 394 125 180 55 417.62 1.32 395 125 181 56 417.65 1.34 396 126 131 5 822.84 5.12 397 126 153 27 541.93 0.86 398 126 178 52 418.57 1.54 399 126 179 53 418.47 1.51 400 126 180 54 417.61 1.32 401 127 131 4 823.26 4.92 402 127 153 26 541.53 0.85 403 127 156 29 540.30 0.80 404 127 157 30 539.00 0.28 405 127 171 44 426.10 0.52 406 127 174 47 424.64 0.95 407 127 178 51 418.54 1.54 408 127 179 52 418.51 1.53 409 127 181 54 417.34 1.34 410 128 131 3 829.88 3.32 411 128 132 4 825.49 3.81 412 128 157 29 536.19 1.03 413 128 171 43 425.93 0.52 414 128 174 46 424.19 1.00 415 128 178 50 418.61 1.56 416 129 132 3 833.20 0.61 417 129 133 4 825.50 3.81 418 129 171 42 425.94 0.52 419 129 174 45 424.20 1.00 420 129 175 46 422.22 1.78 421 130 133 3 828.21 3.29 422 130 134 4 825.49 3.81 423 130 174 44 424.37 0.97 424 130 175 45 424.07 1.01 425 130 176 46 422.14 1.74 426 130 177 47 420.70 1.30 427 130 178 48 418.70 1.60 428 131 134 3 827.32 2.85 429 131 135 4 823.35 4.79 430 131 153 22 541.01 0.96 431 131 178 47 418.62 1.57 432 132 135 3 828.09 4.42 433 132 136 4 829.11 3.90 434 132 153 21 540.49 0.72 435 132 154 22 539.11 0.23 436 132 157 25 505.18 0.32 437 133 136 3 845.65 4.37 438 133 137 4 832.87 2.02 439 134 137 3 844.35 3.71 440 134 138 4 832.87 2.02 441 135 138 3 844.36 3.71 442 135 139 4 832.87 2.02 443 135 150 15 541.16 0.96 444 135 153 18 539.15 0.27 445 136 139 3 864.12 0.39 446 136 140 4 842.97 0.60 447 137 140 3 857.56 2.90 448 137 141 4 854.55 5.18 449 138 141 3 861.93 3.36 450 138 146 8 542.11 0.64 451 138 150 12 541.28 0.99 452 139 147 8 541.91 0.69 453 139 150 11 541.26 1.00 454 139 151 12 541.25 1.00 455 139 154 15 525.10 1.22 456 143 146 3 826.03 2.27 457 143 147 4 817.97 6.32 458 144 147 3 826.86 1.89 459 144 148 4 824.12 3.25 460 145 148 3 846.12 2.75 461 145 149 4 831.13 6.75 462 146 149 3 850.28 2.82 463 146 150 4 833.68 5.48 464 147 150 3 850.28 2.82 465 147 151 4 833.75 5.44 466 148 151 3 849.70 2.54 467 148 152 4 841.39 1.62 468 149 152 3 849.69 2.54 469 149 153 4 841.38 1.61 470 150 153 3 849.69 2.54 471 150 154 4 835.49 1.33 472 151 154 3 828.82 5.63 473 151 155 4 817.86 0.15 474 152 156 4 814.58 1.83 475 153 156 3 846.27 1.55 476 153 157 4 814.58 1.83 477 154 157 3 851.75 1.19 478 154 158 4 822.15 1.96 479 155 158 3 851.75 1.20 480 155 159 4 808.48 1.21 481 155 160 5 806.99 0.46 482 156 160 4 792.38 7.76 483 156 161 5 792.38 7.75 484 156 167 11 427.12 0.90 485 156 181 25 401.15 0.10 486 156 186 30 356.25 8.01 487 156 189 33 330.51 2.84 488 157 161 4 807.56 0.18 489 157 171 14 425.92 0.53 490 158 161 3 853.26 3.10 491 158 162 4 809.96 1.01 492 159 162 3 846.62 6.42 493 159 163 4 817.33 2.69 494 160 163 3 832.73 0.73 495 160 164 4 811.15 0.18 496 160 167 7 569.34 69.56 497 160 189 29 330.41 2.83 498 161 164 3 841.06 5.32 499 161 167 6 569.35 69.56 500 161 168 7 426.09 0.47 501 162 238 76 221.81 1.09 502 162 239 77 221.36 1.22 503 163 192 29 307.75 1.70 504 163 237 74 222.62 0.71 505 163 238 75 221.60 1.11 506 163 239 76 221.25 1.19 507 164 167 3 854.26 2.54 508 164 168 4 835.22 1.75 509 164 188 24 347.88 9.70 510 164 192 28 308.56 1.34 511 164 195 31 308.69 1.27 512 164 236 72 222.67 0.72 513 164 237 73 222.62 0.71 514 164 238 74 222.21 0.92 515 165 168 3 850.47 0.62 516 165 169 4 843.96 2.62 517 165 236 71 222.68 0.72 518 165 238 73 222.53 0.77 519 166 169 3 850.97 3.48 520 166 170 4 843.96 2.62 521 166 182 16 409.47 0.26 522 166 184 18 372.63 3.94 523 166 185 19 364.49 3.91 524 166 188 22 358.18 4.63 525 166 218 52 293.62 1.75 526 166 236 70 222.68 0.72 527 167 170 3 843.95 2.62 528 167 171 4 837.44 0.63 529 167 185 18 364.44 3.92 530 167 188 21 358.16 4.63 531 167 189 22 355.75 5.83 532 168 171 3 844.26 3.64 533 168 172 4 829.88 0.95 534 169 172 3 840.10 1.96 535 169 173 4 830.38 2.90 536 170 173 3 845.55 0.80 537 170 174 4 830.38 2.90 538 170 182 12 410.51 0.78 539 170 185 15 368.94 1.99 540 171 174 3 840.05 1.96 541 171 175 4 830.32 2.91 542 172 175 3 823.51 6.32 543 174 177 3 804.59 4.96 544 174 178 4 560.67 62.11 545 174 179 5 497.79 30.66 546 174 180 6 571.47 67.51 547 174 181 7 418.70 1.60 548 181 185 4 821.85 4.75 549 181 186 5 820.83 5.26 550 182 185 3 835.13 1.87 551 182 186 4 838.75 3.19 552 182 218 36 293.56 1.77 553 183 186 3 845.56 0.21 554 183 187 4 838.27 3.44 555 183 216 33 293.85 1.97 556 183 220 37 293.53 1.73 557 184 187 3 845.56 0.21 558 184 188 4 838.74 3.20 559 184 216 32 293.82 1.97 560 184 217 33 293.86 1.92 561 184 218 34 293.57 1.76 562 185 188 3 846.97 0.24 563 185 189 4 838.79 3.17 564 186 189 3 841.69 2.89 565 186 190 4 835.10 1.92 566 187 190 3 840.65 2.15 567 187 191 4 828.98 1.73 568 187 215 28 293.86 1.99 569 187 216 29 293.88 1.96 570 188 191 3 838.12 3.42 571 188 192 4 820.84 5.23 572 188 215 27 293.86 1.99 573 188 236 48 223.11 0.79 574 189 192 3 855.75 4.12 575 191 194 3 836.36 2.25 576 191 195 4 814.80 6.99 577 191 213 22 293.92 2.04 578 191 215 24 293.88 1.99 579 192 195 3 838.50 3.21 580 192 196 4 834.80 2.70 581 192 237 45 223.34 0.72 582 192 239 47 222.58 0.89 583 192 241 49 212.94 1.00 584 193 196 3 844.61 0.27 585 193 197 4 834.49 2.86 586 193 241 48 214.73 0.07 587 193 246 53 163.56 0.95 588 194 197 3 844.61 0.27 589 194 198 4 836.01 2.08 590 194 213 19 293.94 2.03 591 195 198 3 836.05 2.10 592 195 199 4 831.70 4.25 593 195 209 14 297.19 1.61 594 195 213 18 293.95 2.04 595 195 232 37 225.88 1.93 596 195 237 42 223.44 0.74 597 196 199 3 849.62 4.57 598 196 200 4 837.62 1.30 599 196 237 41 223.61 0.80 600 196 240 44 220.37 1.19 601 196 241 45 220.31 1.16 602 197 200 3 849.59 4.58 603 197 201 4 830.01 2.50 604 197 243 46 207.22 3.81 605 197 246 49 203.88 3.58 606 198 201 3 830.02 2.50 607 198 202 4 821.69 6.66 608 198 209 11 297.19 1.62 609 198 210 12 297.19 1.62 610 198 211 13 297.07 1.60 611 198 213 15 294.28 2.08 612 199 202 3 837.01 1.01 613 199 203 4 837.01 1.01 614 199 209 10 297.20 1.61 615 199 233 34 228.42 0.67 616 199 237 38 223.79 0.83 617 199 240 41 223.44 0.74 618 200 203 3 838.62 0.77 619 200 240 40 223.47 0.73 620 200 241 41 223.44 0.74 621 200 242 42 223.19 0.70 622 200 243 43 222.09 0.77 623 201 205 4 835.51 2.31 624 201 210 9 297.19 1.61 625 202 205 3 816.94 3.98 626 202 206 4 791.75 2.47 627 202 207 5 308.38 3.80 628 202 208 6 308.35 3.78 629 202 209 7 298.37 1.07 630 202 228 26 293.06 1.83 631 204 210 6 694.91 5.87 632 205 208 3 821.25 4.71 633 205 210 5 702.57 1.91 634 207 227 20 293.11 1.83 635 207 228 21 293.06 1.83 636 208 227 19 293.07 1.82 637 209 225 16 293.55 1.70 638 209 226 17 293.08 1.82 639 209 227 18 293.05 1.83 640 209 228 19 293.06 1.83 641 209 233 24 234.72 0.52 642 212 224 12 293.61 1.65 643 212 225 13 293.53 1.62 644 213 224 11 293.56 1.61 645 213 225 12 293.52 1.61 646 214 223 9 293.73 1.59 647 214 224 10 293.56 1.61 648 215 222 7 293.81 1.57 649 215 223 8 293.69 1.55 650 215 225 10 293.64 1.55 651 215 232 17 230.49 1.64 652 216 219 3 894.73 0.53 653 216 220 4 562.53 122.51 654 216 221 5 294.46 1.85 655 216 223 7 293.73 1.54 656 217 223 6 293.82 1.56 657 225 230 5 303.98 0.19 658 225 232 7 302.83 0.77 659 225 233 8 303.41 1.15 660 227 230 3 871.93 1.40 661 228 233 5 306.77 0.02 662 228 234 6 371.13 31.89 663 229 234 5 371.13 31.89 664 230 233 3 831.41 4.21 665 230 234 4 774.32 15.88 666 231 234 3 823.36 3.58 667 231 235 4 614.78 89.93 668 231 236 5 614.77 89.93 669 232 236 4 309.65 1.15 670 232 237 5 308.09 0.37 671 233 237 4 534.83 101.34 672 237 240 3 931.22 0.98 673 241 245 4 843.44 0.06 674 241 246 5 791.39 17.82 675 242 245 3 860.62 0.53 676 243 246 3 851.47 4.04