BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 227 223 113.28 0.93 2 4 229 225 114.20 0.47 3 4 230 226 112.88 1.10 4 5 205 200 178.09 2.09 5 5 206 201 174.19 0.14 6 5 207 202 174.19 0.14 7 5 208 203 162.77 3.98 8 5 230 225 114.59 0.52 9 6 37 31 486.69 92.03 10 6 38 32 486.67 92.03 11 6 39 33 486.67 92.03 12 6 208 202 174.03 0.22 13 7 37 30 486.97 91.90 14 7 38 31 486.68 92.03 15 7 39 32 486.68 92.03 16 7 224 217 114.90 0.52 17 7 230 223 114.89 0.51 18 7 250 243 21.40 0.01 19 8 39 31 486.68 92.03 20 8 41 33 486.69 92.02 21 8 91 83 198.05 1.02 22 8 164 156 186.57 0.60 23 8 208 200 184.29 1.09 24 8 210 202 173.84 0.31 25 8 230 222 114.96 0.52 26 8 231 223 114.89 0.52 27 8 232 224 114.86 0.49 28 9 38 29 486.99 91.89 29 9 39 30 486.94 91.90 30 9 40 31 486.75 91.99 31 9 41 32 486.78 91.98 32 9 232 223 114.90 0.51 33 9 243 234 56.18 0.59 34 9 247 238 43.74 1.54 35 10 41 31 486.78 91.98 36 10 125 115 195.34 1.00 37 10 210 200 186.07 0.59 38 10 232 222 114.96 0.51 39 10 233 223 114.86 0.49 40 10 234 224 114.70 0.57 41 10 243 233 56.18 0.59 42 11 41 30 486.97 91.89 43 11 42 31 486.95 91.89 44 11 43 32 487.09 91.82 45 11 234 223 114.85 0.50 46 11 238 227 85.20 0.36 47 11 243 232 56.18 0.59 48 12 43 31 487.11 91.81 49 12 66 54 300.26 1.10 50 12 96 84 196.55 1.02 51 12 233 221 116.31 0.17 52 12 234 222 114.96 0.51 53 12 235 223 114.69 0.57 54 12 238 226 92.87 0.15 55 13 16 3 870.28 5.79 56 13 22 9 489.53 91.40 57 13 25 12 489.34 91.33 58 13 26 13 489.31 91.35 59 13 42 29 487.22 91.78 60 13 43 30 487.20 91.77 61 13 44 31 487.20 91.77 62 13 52 39 448.63 72.17 63 13 66 53 300.44 1.04 64 13 238 225 113.00 0.29 65 13 239 226 87.14 1.19 66 14 45 31 487.52 91.61 67 14 66 52 300.29 1.10 68 14 96 82 197.83 0.85 69 14 98 84 197.72 0.85 70 14 173 159 197.48 0.92 71 14 235 221 116.18 0.12 72 15 45 30 487.54 91.61 73 16 45 29 487.55 91.61 74 16 239 223 114.60 0.53 75 17 21 4 835.85 3.75 76 17 22 5 824.55 1.89 77 17 48 31 487.95 91.34 78 18 21 3 840.01 5.83 79 18 22 4 837.04 3.16 80 18 48 30 488.01 91.31 81 19 22 3 860.35 6.32 82 19 23 4 837.03 3.16 83 19 51 32 480.22 87.26 84 20 24 4 866.26 3.37 85 21 24 3 896.48 3.25 86 21 25 4 855.72 1.90 87 21 239 218 114.25 0.66 88 22 25 3 855.89 1.81 89 22 26 4 855.72 1.90 90 22 48 26 488.09 91.27 91 22 51 29 462.95 78.62 92 22 239 217 112.56 0.01 93 23 26 3 868.67 4.25 94 23 27 4 855.71 1.90 95 23 55 32 380.37 37.19 96 24 27 3 851.09 4.21 97 24 28 4 852.94 3.35 98 25 28 3 853.54 3.06 99 25 29 4 848.90 0.57 100 25 238 213 114.11 0.52 101 25 239 214 104.95 1.18 102 25 240 215 87.11 1.19 103 25 241 216 84.86 0.50 104 26 29 3 851.85 0.91 105 26 30 4 848.89 0.57 106 26 52 26 439.69 66.92 107 26 55 29 308.93 1.43 108 26 56 30 303.91 1.04 109 27 30 3 852.57 1.27 110 27 31 4 849.92 1.83 111 28 31 3 858.88 2.08 112 28 32 4 848.15 2.72 113 28 241 213 80.41 1.52 114 28 244 216 65.20 2.38 115 29 32 3 864.23 2.31 116 29 42 13 488.35 91.23 117 29 56 27 303.97 1.07 118 29 58 29 303.50 1.30 119 29 61 32 303.23 1.44 120 29 243 214 64.81 2.58 121 29 244 215 56.19 0.58 122 30 55 25 308.92 1.40 123 30 56 26 303.94 1.09 124 30 57 27 303.95 1.13 125 30 58 28 303.51 1.30 126 32 58 26 306.92 3.02 127 32 244 212 56.19 0.57 128 34 38 4 866.58 7.92 129 34 60 26 324.99 12.29 130 34 61 27 324.99 12.29 131 34 244 210 56.18 0.58 132 34 247 213 48.34 0.78 133 34 248 214 45.48 1.90 134 35 60 25 342.48 21.04 135 36 60 24 342.62 21.12 136 36 247 211 48.33 0.80 137 36 250 214 36.02 0.24 138 36 251 215 26.81 0.27 139 38 60 22 341.74 21.57 140 38 247 209 43.88 1.48 141 38 250 212 28.55 0.63 142 39 60 21 341.76 21.56 143 39 62 23 341.72 21.57 144 39 91 52 198.08 1.04 145 40 60 20 342.37 21.27 146 40 61 21 341.74 21.57 147 40 62 22 341.72 21.57 148 40 247 207 43.88 1.48 149 41 62 21 341.76 21.56 150 41 64 23 341.61 21.63 151 41 91 50 198.08 1.04 152 42 52 10 344.55 20.61 153 42 56 14 343.06 21.11 154 42 61 19 342.66 21.29 155 42 62 20 342.37 21.29 156 42 63 21 341.78 21.57 157 42 64 22 341.71 21.59 158 43 64 21 341.63 21.63 159 43 66 23 341.45 21.69 160 43 93 50 199.38 0.36 161 44 48 4 847.40 0.27 162 44 49 5 752.93 38.43 163 44 52 8 537.23 67.93 164 44 63 19 342.65 21.29 165 44 64 20 342.28 21.46 166 44 66 22 341.62 21.62 167 45 48 3 863.51 3.23 168 45 49 4 863.01 3.48 169 45 66 21 341.85 21.64 170 46 49 3 878.13 3.19 171 46 50 4 879.00 2.15 172 46 65 19 342.22 21.50 173 46 67 21 341.94 21.62 174 46 79 33 216.01 2.50 175 46 84 38 215.64 2.52 176 47 50 3 925.93 0.54 177 48 51 3 885.28 5.05 178 48 52 4 852.10 2.00 179 49 52 3 876.92 1.94 180 49 53 4 849.59 0.85 181 49 63 14 342.78 21.28 182 49 65 16 342.51 21.35 183 49 84 35 213.41 2.17 184 49 87 38 211.09 2.56 185 49 89 40 202.50 0.98 186 50 53 3 872.21 1.26 187 50 54 4 845.36 2.94 188 50 87 37 210.79 2.42 189 51 54 3 857.47 8.99 190 51 55 4 845.36 2.94 191 52 55 3 859.45 0.42 192 52 56 4 832.22 0.89 193 52 63 11 342.84 21.27 194 53 63 10 342.86 21.25 195 53 87 34 210.66 2.46 196 53 89 36 200.63 0.45 197 56 61 5 599.45 39.84 198 56 63 7 342.91 21.27 199 58 61 3 854.68 3.92 200 61 243 182 61.40 0.93 201 61 247 186 45.31 0.77 202 62 90 28 200.40 0.53 203 62 91 29 198.71 1.35 204 63 89 26 200.82 0.52 205 63 91 28 200.34 0.55 206 64 89 25 201.19 0.65 207 64 91 27 200.34 0.55 208 64 92 28 200.56 0.60 209 64 93 29 200.44 0.49 210 65 84 19 210.29 2.37 211 65 89 24 201.53 0.78 212 65 91 26 200.83 0.53 213 65 92 27 200.67 0.60 214 65 93 28 200.74 0.58 215 66 93 27 200.88 0.56 216 67 84 17 216.02 2.46 217 67 92 25 201.52 0.75 218 67 93 26 201.44 0.74 219 67 94 27 201.40 0.70 220 67 99 32 201.44 0.71 221 67 113 46 203.23 0.03 222 68 79 11 217.09 1.96 223 68 80 12 217.12 1.94 224 68 81 13 217.13 1.93 225 68 84 16 216.34 2.31 226 68 92 24 201.68 0.74 227 68 94 26 201.58 0.71 228 68 113 45 209.16 2.64 229 69 75 6 217.85 1.71 230 69 78 9 217.41 1.92 231 69 79 10 217.29 1.97 232 69 80 11 217.17 1.94 233 69 81 12 217.25 1.92 234 69 113 44 210.69 2.57 235 70 81 11 217.32 1.89 236 70 82 12 217.63 1.79 237 70 112 42 211.55 2.50 238 70 113 43 211.21 2.58 239 70 116 46 211.87 2.68 240 71 75 4 899.07 2.87 241 71 80 9 217.64 1.79 242 71 82 11 217.65 1.79 243 72 80 8 217.72 1.77 244 72 82 10 217.72 1.76 245 72 83 11 217.73 1.76 246 75 78 3 872.31 4.29 247 79 83 4 851.16 3.66 248 79 84 5 851.16 3.65 249 80 83 3 857.89 1.61 250 80 84 4 851.18 3.66 251 81 84 3 857.37 1.34 252 81 85 4 851.18 3.66 253 81 92 11 316.69 46.69 254 81 113 32 319.86 45.30 255 81 116 35 365.94 29.96 256 81 117 36 319.88 45.29 257 81 122 41 481.89 14.98 258 82 85 3 854.14 2.96 259 82 86 4 851.97 4.05 260 83 86 3 858.78 4.12 261 83 87 4 855.06 2.51 262 84 87 3 857.65 1.22 263 84 88 4 855.06 2.51 264 84 89 5 687.43 67.56 265 84 122 38 531.30 33.95 266 85 88 3 848.12 5.29 267 85 89 4 847.70 5.29 268 85 120 35 545.94 26.33 269 85 122 37 530.98 33.80 270 89 122 33 608.60 29.34 271 90 122 32 608.58 29.35 272 90 123 33 608.58 29.35 273 91 122 31 608.62 29.36 274 91 123 32 608.58 29.35 275 91 125 34 608.58 29.35 276 91 164 73 608.58 29.35 277 92 117 25 608.71 29.36 278 92 122 30 608.67 29.36 279 92 123 31 608.65 29.34 280 92 124 32 608.60 29.34 281 92 125 33 608.60 29.34 282 93 125 32 608.60 29.34 283 93 127 34 608.56 29.36 284 94 113 19 608.77 29.35 285 94 114 20 608.75 29.36 286 94 117 23 608.82 29.38 287 94 124 30 608.69 29.36 288 94 125 31 608.64 29.35 289 94 126 32 608.60 29.34 290 94 127 33 608.58 29.35 291 95 100 5 830.45 1.71 292 95 110 15 608.74 29.36 293 95 127 32 608.61 29.34 294 96 99 3 858.43 1.14 295 96 100 4 853.31 2.87 296 96 127 31 608.64 29.35 297 96 168 72 608.59 29.35 298 96 173 77 551.20 33.23 299 97 100 3 853.32 2.87 300 97 101 4 853.31 2.87 301 97 168 71 608.59 29.35 302 97 170 73 601.21 30.02 303 97 173 76 551.20 33.23 304 98 101 3 873.34 0.01 305 98 102 4 837.31 5.13 306 98 103 5 837.30 5.14 307 98 173 75 566.86 28.93 308 99 103 4 771.53 38.02 309 99 104 5 771.52 38.02 310 99 105 6 628.19 19.65 311 100 105 5 609.04 29.22 312 100 107 7 609.04 29.22 313 100 110 10 609.13 29.27 314 100 127 27 608.80 29.34 315 100 147 47 608.96 29.27 316 101 170 69 607.74 29.59 317 105 109 4 830.88 4.75 318 105 110 5 823.28 0.95 319 105 113 8 758.59 33.25 320 106 109 3 862.78 5.19 321 106 110 4 847.97 0.96 322 107 110 3 855.25 0.17 323 107 111 4 847.99 0.96 324 107 146 39 609.23 29.26 325 107 147 40 609.09 29.31 326 107 150 43 609.42 29.36 327 108 111 3 853.21 1.62 328 108 112 4 848.38 0.79 329 109 112 3 855.06 1.11 330 109 113 4 851.39 0.71 331 110 113 3 858.20 1.38 332 110 114 4 851.39 0.71 333 110 126 16 609.27 29.25 334 110 151 41 609.68 29.37 335 111 114 3 876.61 0.58 336 111 115 4 848.25 0.84 337 111 150 39 609.63 29.34 338 111 151 40 609.60 29.36 339 111 154 43 610.13 29.30 340 112 115 3 876.61 0.58 341 112 116 4 864.95 3.97 342 112 154 42 610.35 29.20 343 113 116 3 875.89 0.22 344 113 117 4 864.95 3.97 345 114 117 3 868.41 3.77 346 114 118 4 853.50 1.76 347 114 124 10 609.72 29.37 348 114 126 12 609.57 29.36 349 114 151 37 610.11 29.28 350 114 155 41 610.65 29.09 351 115 118 3 865.65 5.16 352 115 119 4 840.72 2.90 353 115 154 39 610.43 29.16 354 115 155 40 610.43 29.16 355 116 119 3 863.62 4.15 356 116 120 4 840.38 2.73 357 117 120 3 862.77 4.26 358 117 121 4 840.38 2.73 359 117 122 5 681.53 0.23 360 117 124 7 610.40 29.16 361 118 121 3 862.76 4.25 362 118 124 6 674.97 3.10 363 118 155 37 709.73 3.83 364 118 159 41 676.56 2.53 365 118 162 44 676.48 2.50 366 118 163 45 675.58 2.80 367 121 162 41 803.92 0.40 368 122 162 40 803.94 0.40 369 123 162 39 803.93 0.41 370 124 162 38 803.99 0.40 371 124 163 39 803.95 0.40 372 124 164 40 803.94 0.40 373 125 164 39 803.94 0.40 374 125 166 41 803.80 0.41 375 125 210 85 435.24 25.17 376 125 233 108 116.50 0.27 377 126 151 25 804.18 0.40 378 126 163 37 804.02 0.40 379 126 164 38 803.98 0.40 380 126 165 39 803.94 0.40 381 126 166 40 803.94 0.40 382 127 166 39 803.94 0.40 383 127 168 41 803.92 0.40 384 127 233 106 117.55 0.43 385 128 144 16 804.39 0.38 386 128 147 19 804.21 0.37 387 128 148 20 804.21 0.38 388 128 165 37 804.04 0.39 389 128 166 38 803.98 0.40 390 128 167 39 803.95 0.40 391 128 168 40 803.94 0.40 392 128 191 63 452.80 22.45 393 129 143 14 804.42 0.38 394 129 144 15 804.41 0.38 395 129 147 18 804.33 0.35 396 129 167 38 803.97 0.40 397 129 168 39 803.95 0.39 398 130 134 4 818.65 6.88 399 130 135 5 811.37 3.23 400 130 140 10 804.47 0.40 401 130 143 13 804.43 0.38 402 130 144 14 804.43 0.38 403 130 167 37 804.03 0.38 404 130 169 39 803.94 0.40 405 130 170 40 803.93 0.40 406 130 171 41 803.93 0.40 407 131 134 3 859.72 6.17 408 131 135 4 858.94 6.55 409 131 170 39 803.94 0.40 410 131 171 40 803.93 0.40 411 132 135 3 859.68 6.68 412 132 136 4 839.13 3.35 413 132 171 39 803.94 0.39 414 133 136 3 865.21 3.42 415 133 137 4 840.35 2.73 416 134 137 3 858.26 0.05 417 134 138 4 840.34 2.73 418 134 139 5 804.84 0.36 419 134 140 6 804.80 0.38 420 134 143 9 804.78 0.39 421 135 138 3 846.62 5.87 422 135 140 5 804.79 0.38 423 135 167 32 804.16 0.38 424 135 171 36 803.98 0.40 425 135 183 48 454.22 22.70 426 135 187 52 453.65 22.48 427 139 142 3 855.20 3.91 428 139 143 4 859.34 1.84 429 140 143 3 859.34 1.84 430 140 144 4 859.34 1.84 431 140 187 47 453.09 22.40 432 141 144 3 882.76 5.32 433 141 145 4 858.60 1.47 434 141 190 49 453.01 22.41 435 142 145 3 864.41 3.86 436 142 146 4 854.12 3.73 437 143 146 3 864.18 3.74 438 143 147 4 853.83 3.88 439 144 147 3 854.15 3.71 440 144 148 4 854.31 3.64 441 144 167 23 804.07 0.41 442 144 187 43 453.00 22.40 443 144 190 46 452.94 22.41 444 144 191 47 452.92 22.41 445 145 148 3 854.31 3.64 446 145 149 4 852.40 2.69 447 145 190 45 452.95 22.42 448 145 191 46 452.83 22.45 449 145 194 49 452.17 22.37 450 146 149 3 859.59 1.47 451 146 150 4 856.03 0.89 452 147 150 3 871.42 0.95 453 147 151 4 859.52 2.62 454 148 151 3 873.11 0.89 455 148 152 4 865.43 4.65 456 148 160 12 804.41 0.32 457 148 165 17 804.09 0.41 458 148 191 43 452.80 22.44 459 148 194 46 452.16 22.37 460 148 195 47 451.46 22.06 461 149 152 3 865.88 4.51 462 149 153 4 865.52 4.70 463 149 194 45 452.14 22.37 464 150 153 3 873.83 0.55 465 150 154 4 865.53 4.69 466 151 154 3 880.08 2.58 467 151 155 4 865.52 4.70 468 152 155 3 872.85 1.03 469 152 160 8 804.57 0.36 470 152 194 42 452.10 22.38 471 155 159 4 863.39 0.70 472 155 160 5 865.62 1.83 473 155 163 8 806.26 0.33 474 156 159 3 874.39 0.21 475 156 160 4 874.15 0.09 476 157 160 3 891.14 1.33 477 157 199 42 436.59 25.16 478 157 203 46 436.16 25.12 479 158 161 3 904.62 1.73 480 158 162 4 885.81 1.33 481 159 162 3 889.89 0.70 482 159 163 4 871.81 1.25 483 160 163 3 876.74 3.20 484 160 165 5 876.74 3.20 485 160 199 39 436.60 25.18 486 160 203 43 435.91 25.25 487 160 207 47 435.66 25.23 488 163 207 44 435.67 25.23 489 164 207 43 435.69 25.24 490 164 208 44 435.45 25.22 491 164 210 46 435.25 25.17 492 165 191 26 452.46 22.55 493 165 207 42 435.78 25.23 494 165 208 43 435.59 25.28 495 165 209 44 435.38 25.20 496 165 210 45 435.35 25.21 497 166 210 44 435.31 25.17 498 166 233 67 117.14 0.30 499 167 187 20 452.75 22.46 500 167 188 21 452.75 22.46 501 167 191 24 452.68 22.45 502 167 210 43 435.49 25.24 503 167 211 44 435.33 25.23 504 167 212 45 435.19 25.17 505 168 173 5 806.65 0.27 506 168 212 44 435.16 25.16 507 169 172 3 863.47 0.35 508 169 173 4 855.72 4.76 509 169 179 10 453.86 22.59 510 169 211 42 435.51 25.25 511 169 212 43 435.42 25.22 512 170 173 3 864.62 2.20 513 171 175 4 842.79 9.24 514 171 177 6 662.22 72.44 515 171 179 8 454.69 22.82 516 171 183 12 454.56 22.85 517 172 175 3 870.98 0.23 518 172 177 5 459.50 25.25 519 172 178 6 454.65 22.84 520 172 213 41 434.95 25.26 521 172 214 42 434.95 25.26 522 176 214 38 435.11 25.21 523 177 214 37 435.35 25.25 524 178 211 33 435.68 25.23 525 178 213 35 435.42 25.23 526 178 214 36 435.36 25.25 527 178 219 41 400.56 26.44 528 178 223 45 331.06 43.55 529 179 183 4 841.74 5.90 530 179 184 5 817.44 6.25 531 179 211 32 435.76 25.27 532 179 223 44 186.38 28.55 533 180 183 3 853.48 2.72 534 180 184 4 860.47 0.01 535 181 184 3 861.35 0.42 536 181 185 4 854.13 3.18 537 181 222 41 185.15 28.96 538 181 223 42 185.08 28.99 539 181 226 45 123.45 0.39 540 182 185 3 868.07 3.38 541 182 186 4 854.14 3.18 542 183 186 3 866.88 2.49 543 183 187 4 863.33 1.41 544 184 187 3 871.41 0.23 545 184 188 4 854.85 2.81 546 184 211 27 435.85 25.30 547 184 223 39 183.13 29.94 548 184 229 45 121.42 0.11 549 185 188 3 865.63 2.21 550 185 189 4 854.84 2.81 551 185 226 41 121.78 0.07 552 185 227 42 121.78 0.07 553 185 228 43 121.53 0.15 554 185 229 44 121.42 0.11 555 186 189 3 863.56 1.55 556 186 190 4 854.84 2.81 557 187 190 3 854.98 1.23 558 187 191 4 850.77 1.10 559 188 191 3 865.60 0.16 560 188 192 4 856.93 4.17 561 188 209 21 436.22 25.21 562 188 210 22 436.18 25.23 563 188 211 23 436.03 25.29 564 188 228 40 121.12 0.24 565 188 229 41 121.04 0.21 566 189 192 3 871.96 0.36 567 189 193 4 856.91 4.18 568 189 227 38 121.53 0.18 569 189 228 39 121.41 0.12 570 190 193 3 867.34 5.22 571 190 194 4 850.77 1.11 572 191 194 3 867.20 2.00 573 191 195 4 854.65 3.08 574 191 209 18 436.20 25.21 575 192 195 3 860.55 0.14 576 192 196 4 854.64 3.08 577 192 204 12 436.56 25.22 578 192 207 15 436.38 25.25 579 192 209 17 436.22 25.21 580 192 228 36 121.05 0.22 581 192 230 38 121.06 0.24 582 193 196 3 861.49 0.18 583 193 197 4 854.62 3.10 584 194 197 3 867.45 2.27 585 194 198 4 850.93 1.09 586 195 198 3 855.84 3.54 587 195 199 4 833.62 2.58 588 195 200 5 769.16 34.81 589 195 203 8 450.31 32.05 590 195 204 9 436.71 25.30 591 195 207 12 436.36 25.25 592 196 199 3 868.66 1.66 593 196 200 4 513.95 34.86 594 196 201 5 497.47 35.12 595 196 204 8 436.75 25.29 596 199 203 4 818.60 3.55 597 200 204 4 849.07 8.17 598 201 204 3 849.08 8.17 599 201 205 4 828.10 2.31 600 202 205 3 856.01 4.69 601 202 206 4 849.07 8.17 602 203 206 3 855.27 6.61 603 203 207 4 846.70 6.98 604 204 207 3 852.52 7.46 605 207 230 23 120.06 0.24 606 208 230 22 119.67 0.08 607 209 228 19 120.60 0.01 608 209 229 20 120.56 0.03 609 209 230 21 120.20 0.15 610 210 230 20 119.87 0.02 611 210 231 21 119.62 0.06 612 210 232 22 118.66 0.08 613 211 215 4 881.08 4.24 614 211 223 12 120.65 0.04 615 211 229 18 120.59 0.01 616 211 231 20 119.86 0.03 617 211 232 21 119.30 0.10 618 211 233 22 118.66 0.08 619 212 215 3 891.94 0.40 620 212 233 21 118.43 0.54 621 214 219 5 839.33 0.68 622 215 219 4 839.32 0.69 623 215 220 5 489.15 29.86 624 215 223 8 455.60 22.12 625 215 232 17 120.03 0.11 626 215 234 19 119.70 0.04 627 215 237 22 117.97 0.42 628 215 246 31 54.38 0.29 629 216 219 3 861.99 5.00 630 216 220 4 769.21 29.11 631 216 237 21 118.40 0.31 632 216 242 26 58.01 0.54 633 216 246 30 54.61 0.18 634 217 220 3 858.32 4.78 635 217 242 25 56.34 0.51 636 217 245 28 56.09 0.55 637 217 246 29 54.55 0.20 638 219 222 3 877.21 2.29 639 219 223 4 858.41 4.79 640 220 223 3 862.46 2.76 641 220 224 4 854.86 6.56 642 220 232 12 121.03 0.17 643 220 246 26 49.48 1.17 644 220 249 29 39.94 3.53 645 221 224 3 862.47 2.76 646 221 225 4 858.40 4.79 647 221 249 28 41.56 2.71 648 221 251 30 38.45 4.22 649 222 225 3 862.65 2.67 650 222 226 4 865.18 4.17 651 223 226 3 890.24 8.37 652 223 229 6 121.67 0.15 653 223 232 9 121.45 0.10 654 224 229 5 123.55 0.81 655 224 230 6 121.64 0.15 656 224 231 7 121.55 0.14 657 224 232 8 121.55 0.14 658 224 249 25 38.43 4.21 659 224 250 26 34.98 5.20 660 226 229 3 867.90 7.15 661 231 250 19 21.29 0.07 662 232 246 14 47.79 1.06 663 232 250 18 24.59 0.24 664 234 237 3 897.22 0.82 665 234 238 4 835.46 7.17 666 234 243 9 56.19 0.58 667 234 246 12 51.61 0.59 668 235 238 3 867.35 1.06 669 236 239 3 888.58 2.90 670 236 240 4 867.22 0.53 671 237 240 3 867.22 0.53 672 237 242 5 490.32 38.05 673 237 243 6 230.89 54.58 674 237 246 9 135.53 40.12 675 238 243 5 469.29 29.27 676 240 243 3 874.83 4.53 677 241 244 3 888.81 2.46 678 241 245 4 865.97 0.88 679 242 245 3 869.58 0.78 680 242 246 4 860.99 3.37 681 243 246 3 869.40 0.86 682 243 247 4 860.94 3.37 683 244 247 3 865.48 5.63 684 244 248 4 860.93 3.37 685 245 248 3 854.76 6.78 686 245 249 4 856.02 6.04 687 246 249 3 869.38 0.63 688 246 250 4 856.13 6.00 689 247 250 3 872.84 1.09 690 248 251 3 877.90 0.48