BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 17 15 39.66 0.39 2 2 20 18 35.78 2.29 3 2 21 19 29.14 0.22 4 2 24 22 29.14 0.21 5 2 49 47 35.09 2.66 6 2 50 48 35.27 2.58 7 2 51 49 40.65 0.72 8 3 17 14 41.52 0.99 9 3 51 48 41.33 1.06 10 4 9 5 784.21 16.51 11 4 13 9 116.62 30.48 12 4 14 10 104.91 28.00 13 4 17 13 41.94 0.89 14 4 51 47 42.03 0.87 15 4 52 48 44.50 0.35 16 4 53 49 56.93 0.01 17 4 127 123 58.91 0.90 18 4 130 126 58.50 0.73 19 5 8 3 856.62 1.43 20 5 9 4 828.57 5.25 21 5 53 48 58.77 0.91 22 6 9 3 845.02 2.98 23 6 53 47 59.08 0.98 24 6 54 48 59.27 1.07 25 6 55 49 59.70 1.27 26 6 127 121 58.92 0.90 27 6 130 124 58.96 0.92 28 6 131 125 59.02 0.95 29 6 134 128 59.39 1.12 30 7 55 48 59.72 1.28 31 7 57 50 60.08 1.26 32 9 13 4 814.48 1.99 33 10 13 3 848.00 1.54 34 10 14 4 830.30 1.65 35 10 173 163 100.77 0.58 36 11 14 3 846.47 0.78 37 11 15 4 832.93 0.35 38 11 170 159 90.31 0.18 39 11 173 162 104.89 0.01 40 12 15 3 848.26 0.12 41 12 16 4 832.57 0.53 42 13 16 3 836.22 1.85 43 13 17 4 832.12 0.30 44 14 17 3 841.98 5.19 45 14 18 4 832.13 0.30 46 14 127 113 115.74 0.01 47 14 155 141 109.50 0.00 48 14 159 145 106.03 0.36 49 14 166 152 106.03 0.36 50 14 169 155 114.29 0.58 51 14 170 156 114.27 0.57 52 15 18 3 848.90 0.12 53 15 19 4 832.13 0.30 54 15 166 151 108.08 0.67 55 15 170 155 114.68 0.50 56 16 19 3 846.50 1.08 57 16 20 4 829.82 1.45 58 17 20 3 838.93 2.71 59 17 21 4 829.70 1.64 60 17 50 33 125.33 0.24 61 17 52 35 129.09 1.25 62 17 156 139 116.77 0.15 63 18 21 3 832.99 3.12 64 18 22 4 833.41 2.92 65 18 155 137 119.87 1.10 66 18 156 138 116.78 0.14 67 18 159 141 116.09 0.18 68 18 160 142 114.70 0.51 69 18 166 148 116.19 0.14 70 19 22 3 851.65 1.53 71 19 23 4 844.77 1.91 72 19 163 144 119.56 0.98 73 20 23 3 844.77 1.91 74 20 24 4 845.01 2.03 75 21 24 3 860.63 2.67 76 21 25 4 846.08 2.56 77 21 48 27 141.09 1.10 78 21 50 29 133.31 1.99 79 21 64 43 132.47 1.96 80 21 69 48 126.27 0.12 81 21 156 135 129.14 1.23 82 21 157 136 128.57 0.98 83 21 160 139 126.72 0.11 84 22 25 3 879.88 0.95 85 22 159 137 131.10 2.21 86 22 160 138 128.46 0.93 87 22 163 141 131.10 2.20 88 22 166 144 131.75 2.15 89 22 236 214 126.13 0.14 90 22 237 215 126.12 0.15 91 24 48 24 147.75 0.27 92 25 37 12 153.19 2.88 93 25 48 23 147.77 0.27 94 25 64 39 147.75 0.27 95 25 66 41 147.32 0.48 96 25 69 44 146.41 0.94 97 25 237 212 135.39 0.49 98 26 37 11 153.19 2.87 99 26 38 12 153.23 2.85 100 26 41 15 152.19 2.31 101 26 45 19 148.03 0.26 102 26 66 40 147.88 0.21 103 27 30 3 945.36 2.64 104 27 35 8 153.43 3.11 105 27 36 9 153.31 2.93 106 27 37 10 153.19 2.87 107 27 38 11 153.24 2.85 108 28 36 8 153.62 3.04 109 28 38 10 153.28 2.86 110 29 36 7 155.31 3.83 111 30 33 3 802.07 1.95 112 30 34 4 492.73 115.95 113 30 35 5 162.37 7.35 114 30 36 6 162.37 7.35 115 31 34 3 825.10 9.65 116 31 36 5 435.10 143.72 117 31 197 166 147.55 0.06 118 31 198 167 147.44 0.10 119 31 201 170 147.87 0.12 120 31 238 207 145.99 0.62 121 32 197 165 147.69 0.01 122 32 200 168 147.94 0.10 123 32 201 169 147.91 0.09 124 32 205 173 147.93 0.10 125 32 226 194 147.90 0.07 126 34 197 163 149.34 0.76 127 34 231 197 149.53 1.81 128 34 235 201 149.85 2.19 129 34 236 202 149.65 2.32 130 34 237 203 149.63 2.33 131 34 238 204 149.45 2.35 132 35 236 201 149.69 2.35 133 35 237 202 149.32 2.52 134 35 238 203 148.80 2.75 135 36 238 202 147.78 3.29 136 36 239 203 147.78 3.29 137 36 240 204 146.47 3.88 138 36 241 205 145.12 4.53 139 37 66 29 151.66 1.54 140 37 68 31 151.56 1.53 141 37 69 32 151.63 1.55 142 37 237 200 148.97 2.84 143 37 238 201 147.69 3.33 144 37 240 203 142.60 5.80 145 38 41 3 857.86 0.69 146 38 67 29 151.63 1.55 147 38 240 202 141.31 6.52 148 39 67 28 151.65 1.55 149 39 240 201 141.33 6.52 150 39 241 202 141.32 6.52 151 39 242 203 137.25 8.46 152 41 45 4 834.75 7.27 153 41 46 5 741.54 3.62 154 41 66 25 663.91 22.86 155 41 67 26 631.07 6.42 156 42 45 3 847.97 4.08 157 42 46 4 829.65 9.80 158 43 46 3 829.65 9.80 159 43 89 46 606.91 5.62 160 43 93 50 606.93 5.62 161 45 66 21 733.42 6.29 162 46 65 19 733.47 6.29 163 46 66 20 733.42 6.29 164 46 67 21 733.40 6.29 165 46 87 41 708.15 0.04 166 46 89 43 707.79 0.04 167 47 64 17 733.56 6.31 168 47 65 18 733.47 6.28 169 47 66 19 733.49 6.29 170 47 72 25 732.03 6.73 171 47 87 40 708.28 0.01 172 47 89 42 708.12 0.03 173 47 90 43 707.85 0.07 174 47 93 46 707.70 0.00 175 47 94 47 706.45 0.09 176 48 63 15 733.65 6.29 177 48 64 16 733.57 6.30 178 48 66 18 733.52 6.30 179 49 61 12 733.86 6.28 180 49 62 13 733.81 6.29 181 49 63 14 733.66 6.29 182 49 94 45 708.44 0.01 183 49 95 46 707.93 0.05 184 50 61 11 733.88 6.28 185 50 62 12 733.82 6.29 186 50 64 14 733.79 6.28 187 50 156 106 446.79 0.18 188 51 59 8 734.07 6.32 189 51 60 9 734.01 6.32 190 52 58 6 734.39 6.21 191 52 59 7 734.12 6.32 192 52 60 8 734.03 6.33 193 52 62 10 733.97 6.32 194 52 134 82 557.64 0.31 195 52 152 100 529.09 0.20 196 52 156 104 462.85 0.51 197 53 57 4 754.09 0.58 198 53 58 5 734.39 6.21 199 53 59 6 734.12 6.32 200 54 57 3 812.53 5.19 201 54 58 4 734.40 6.21 202 54 131 77 557.82 0.30 203 54 134 80 557.83 0.30 204 54 135 81 557.82 0.29 205 55 127 72 558.00 0.27 206 55 128 73 557.99 0.28 207 55 131 76 557.88 0.29 208 58 134 76 557.81 0.29 209 58 135 77 557.81 0.29 210 58 138 80 557.72 0.33 211 58 139 81 557.79 0.29 212 59 138 79 557.70 0.33 213 60 74 14 731.88 6.63 214 60 75 15 731.02 6.29 215 60 134 74 557.59 0.30 216 60 137 77 557.68 0.30 217 60 138 78 557.56 0.32 218 61 74 13 731.37 6.38 219 61 75 14 730.56 6.34 220 61 95 34 708.60 0.04 221 62 71 9 732.81 6.58 222 62 73 11 731.83 6.61 223 62 74 12 730.36 6.44 224 62 152 90 516.56 1.02 225 63 71 8 732.78 6.58 226 63 72 9 732.74 6.56 227 63 73 10 731.35 6.37 228 63 94 31 708.44 0.01 229 63 95 32 708.18 0.07 230 64 69 5 733.68 6.65 231 64 70 6 733.02 6.46 232 64 71 7 732.74 6.56 233 65 68 3 858.18 4.62 234 65 69 4 735.84 5.57 235 65 70 5 733.51 6.71 236 65 72 7 732.03 6.73 237 67 240 173 143.75 7.73 238 68 161 93 440.02 0.34 239 68 190 122 229.50 0.48 240 68 239 171 149.39 5.11 241 68 240 172 145.28 7.16 242 69 157 88 443.12 1.34 243 69 161 92 439.89 0.27 244 69 190 121 286.48 16.92 245 69 237 168 152.35 4.02 246 70 161 91 441.55 1.04 247 71 83 12 709.07 0.04 248 71 153 82 483.70 2.21 249 72 82 10 709.08 0.04 250 72 83 11 709.07 0.04 251 72 87 15 708.70 0.04 252 72 90 18 708.64 0.06 253 72 94 22 708.62 0.06 254 73 81 8 709.28 0.03 255 73 82 9 709.09 0.04 256 73 94 21 708.63 0.06 257 73 95 22 708.62 0.05 258 74 80 6 718.09 4.28 259 74 81 7 709.28 0.01 260 74 95 21 708.61 0.04 261 74 137 63 557.57 0.29 262 74 140 66 557.39 0.34 263 75 78 3 878.44 0.51 264 75 79 4 805.92 4.84 265 75 80 5 709.43 0.04 266 75 95 20 708.60 0.04 267 75 98 23 707.79 0.04 268 76 79 3 846.85 0.79 269 76 81 5 795.35 0.80 270 76 137 61 557.62 0.28 271 76 138 62 557.55 0.32 272 77 138 61 557.69 0.32 273 77 139 62 557.69 0.32 274 78 98 20 707.81 0.04 275 78 99 21 707.81 0.04 276 79 98 19 707.80 0.04 277 79 99 20 707.75 0.05 278 79 101 22 704.82 1.09 279 80 95 15 708.73 0.01 280 80 98 18 707.79 0.04 281 80 99 19 707.70 0.06 282 80 100 20 688.00 1.08 283 80 101 21 686.48 0.35 284 81 101 20 675.47 2.62 285 81 103 22 658.59 0.98 286 81 140 59 557.25 0.27 287 82 86 4 833.71 10.61 288 82 90 8 708.93 0.07 289 82 91 9 708.81 0.04 290 82 94 12 708.79 0.02 291 82 95 13 708.78 0.02 292 82 100 18 682.85 1.46 293 82 101 19 675.47 2.62 294 82 102 20 658.57 0.99 295 82 103 21 658.56 0.99 296 83 86 3 853.02 0.96 297 83 103 20 658.51 1.02 298 83 105 22 647.62 6.37 299 83 106 23 560.55 0.04 300 83 107 24 560.52 0.02 301 83 112 29 560.29 0.13 302 83 149 66 553.00 0.67 303 84 102 18 659.02 1.06 304 84 103 19 658.56 0.99 305 84 104 20 658.49 1.01 306 84 105 21 644.57 7.90 307 84 106 22 585.67 12.51 308 85 106 21 585.67 12.51 309 86 90 4 838.66 0.27 310 86 91 5 838.65 0.27 311 87 90 3 855.13 3.38 312 87 91 4 860.46 0.72 313 88 91 3 860.56 0.67 314 88 92 4 860.46 0.72 315 89 92 3 848.59 5.21 316 89 93 4 838.85 0.35 317 90 93 3 852.68 4.71 318 90 94 4 835.50 0.86 319 91 100 9 680.63 2.57 320 91 102 11 659.31 0.83 321 92 96 4 855.50 1.42 322 93 96 3 899.17 0.96 323 94 100 6 820.06 2.41 324 95 98 3 867.10 1.37 325 95 100 5 834.09 1.62 326 96 100 4 879.61 3.76 327 103 140 37 557.25 0.28 328 105 111 6 576.41 7.91 329 105 112 7 560.60 0.00 330 105 115 10 560.43 0.07 331 105 140 35 557.45 0.34 332 105 145 40 556.47 0.45 333 105 146 41 556.14 0.42 334 105 149 44 555.48 0.17 335 106 111 5 772.39 5.27 336 107 112 5 803.78 3.30 337 108 111 3 850.05 1.24 338 108 112 4 826.26 2.37 339 109 112 3 844.76 3.88 340 109 113 4 826.34 2.34 341 109 120 11 558.61 0.27 342 109 150 41 556.00 0.42 343 109 153 44 555.00 0.33 344 109 154 45 554.89 0.38 345 109 158 49 504.06 1.67 346 109 187 78 330.25 4.99 347 110 113 3 842.74 4.88 348 110 114 4 826.62 2.48 349 111 114 3 840.79 0.36 350 111 115 4 827.50 2.02 351 112 115 3 862.57 0.68 352 112 116 4 826.59 2.47 353 112 146 34 556.41 0.45 354 112 149 37 556.19 0.51 355 112 150 38 555.90 0.54 356 112 153 41 554.99 0.34 357 113 116 3 827.82 1.89 358 113 117 4 822.41 4.55 359 113 118 5 814.37 8.54 360 113 119 6 558.79 0.34 361 113 120 7 558.83 0.36 362 113 150 37 556.20 0.51 363 114 117 3 827.49 2.02 364 115 146 31 556.51 0.45 365 116 146 30 556.52 0.44 366 116 147 31 556.49 0.43 367 116 150 34 556.45 0.42 368 118 122 4 835.50 0.36 369 118 123 5 787.16 1.68 370 119 122 3 867.34 6.94 371 120 123 3 833.31 5.13 372 120 150 30 556.23 0.47 373 120 154 34 555.25 0.21 374 120 179 59 438.39 1.78 375 120 184 64 364.80 0.82 376 120 187 67 358.57 2.02 377 120 188 68 358.99 1.81 378 121 178 57 439.78 1.55 379 121 179 58 438.44 1.80 380 121 184 63 371.36 2.46 381 122 178 56 440.17 1.74 382 123 147 24 556.53 0.42 383 123 150 27 555.48 0.14 384 123 151 28 555.37 0.15 385 123 178 55 439.81 1.54 386 123 179 56 439.56 1.46 387 124 129 5 805.47 8.71 388 124 151 27 555.24 0.20 389 124 176 52 440.56 1.81 390 124 177 53 440.49 1.80 391 124 178 54 439.87 1.56 392 125 129 4 818.25 2.35 393 125 151 26 554.75 0.35 394 125 154 29 553.66 0.66 395 125 155 30 551.76 0.83 396 125 176 51 440.53 1.79 397 125 177 52 440.49 1.80 398 125 179 54 439.55 1.46 399 126 129 3 827.95 1.97 400 126 130 4 827.81 2.05 401 126 155 29 551.70 0.80 402 126 172 46 441.81 1.45 403 126 175 49 440.66 1.79 404 126 176 50 440.53 1.79 405 126 177 51 440.53 1.79 406 127 130 3 836.12 0.90 407 127 131 4 827.89 2.01 408 127 169 42 442.06 1.39 409 127 172 45 441.81 1.45 410 127 173 46 441.62 1.49 411 128 131 3 845.40 0.96 412 128 132 4 830.62 3.08 413 128 172 44 441.82 1.44 414 128 173 45 441.67 1.47 415 128 174 46 441.18 1.65 416 128 175 47 440.69 1.73 417 128 176 48 440.62 1.77 418 129 132 3 849.24 7.14 419 129 133 4 835.87 0.47 420 129 151 22 554.73 0.35 421 129 176 47 440.54 1.79 422 130 133 3 845.65 1.13 423 130 134 4 835.87 0.47 424 130 151 21 554.11 0.56 425 130 152 22 552.01 0.77 426 130 155 25 526.05 1.72 427 131 134 3 840.96 1.26 428 131 135 4 839.22 2.14 429 132 135 3 837.02 3.24 430 132 136 4 834.02 4.75 431 133 136 3 839.26 2.13 432 133 137 4 831.78 5.87 433 133 148 15 554.33 0.58 434 133 151 18 551.97 0.88 435 134 137 3 839.26 2.13 436 134 138 4 831.78 5.87 437 135 138 3 841.86 0.83 438 135 139 4 818.65 12.43 439 136 140 4 800.00 9.22 440 136 144 8 556.49 0.42 441 136 148 12 554.81 0.35 442 137 140 3 803.61 11.03 443 137 145 8 555.74 0.23 444 137 148 11 554.75 0.35 445 137 149 12 554.74 0.35 446 137 152 15 541.54 1.11 447 140 145 5 806.00 3.34 448 141 144 3 850.02 6.03 449 141 145 4 831.31 0.36 450 142 145 3 867.59 4.25 451 142 146 4 860.57 0.75 452 143 146 3 908.48 2.56 453 144 147 3 887.28 2.86 454 144 148 4 845.14 5.98 455 145 148 3 851.28 2.92 456 145 149 4 836.40 1.60 457 146 149 3 839.75 1.22 458 146 150 4 833.82 1.28 459 147 150 3 832.22 0.48 460 147 151 4 853.74 4.20 461 148 151 3 853.75 4.20 462 148 152 4 853.75 4.19 463 149 152 3 854.26 3.94 464 149 153 4 840.27 2.56 465 150 153 3 868.81 3.34 466 150 154 4 840.28 2.56 467 151 154 3 873.37 5.60 468 151 155 4 840.29 2.55 469 152 155 3 858.50 2.27 470 152 156 4 839.71 2.04 471 153 156 3 839.71 2.04 472 153 157 4 807.63 4.50 473 153 158 5 803.86 6.39 474 154 158 4 788.15 7.50 475 154 159 5 798.41 2.38 476 154 165 11 442.66 1.15 477 154 187 33 323.45 1.50 478 155 159 4 811.34 6.55 479 155 169 14 441.99 1.41 480 156 159 3 846.43 10.97 481 156 160 4 830.24 2.89 482 157 160 3 842.24 5.89 483 157 161 4 826.07 6.98 484 158 161 3 840.89 6.00 485 158 162 4 826.05 7.00 486 158 165 7 598.51 79.08 487 158 187 29 324.07 1.08 488 159 162 3 840.94 5.98 489 159 165 6 445.51 2.58 490 159 166 7 442.44 1.20 491 160 236 76 176.11 15.55 492 160 237 77 174.28 14.98 493 161 190 29 321.12 0.39 494 161 235 74 176.33 15.46 495 161 236 75 176.12 15.55 496 161 237 76 173.79 15.18 497 162 165 3 840.25 5.83 498 162 166 4 829.50 0.46 499 162 186 24 331.94 4.25 500 162 187 25 331.99 4.21 501 162 190 28 321.74 0.58 502 162 193 31 321.28 0.36 503 162 234 72 176.38 15.47 504 162 235 73 176.33 15.46 505 163 166 3 830.01 0.71 506 163 167 4 829.50 0.46 507 163 234 71 176.19 15.56 508 163 236 73 176.14 15.57 509 164 167 3 850.28 1.91 510 164 168 4 850.28 1.91 511 164 180 16 435.36 3.32 512 164 182 18 368.68 0.95 513 164 183 19 356.34 2.56 514 164 186 22 345.70 2.04 515 164 216 52 318.25 2.71 516 164 234 70 176.18 15.58 517 165 168 3 854.24 0.07 518 165 169 4 850.28 1.91 519 165 183 18 346.16 1.81 520 165 186 21 332.06 4.24 521 165 187 22 332.05 4.23 522 166 169 3 863.46 4.67 523 166 170 4 842.36 3.33 524 167 170 3 847.74 0.64 525 167 171 4 842.37 3.32 526 168 171 3 846.31 3.91 527 168 172 4 839.06 1.67 528 168 177 9 440.60 1.84 529 168 180 12 439.24 1.84 530 168 183 15 372.51 1.71 531 169 172 3 857.51 2.26 532 169 177 8 440.60 1.84 533 170 173 3 880.52 1.73 534 172 175 3 842.21 3.36 535 172 176 4 740.93 5.92 536 172 177 5 539.28 47.49 537 177 183 6 817.69 1.01 538 179 183 4 795.00 6.55 539 179 184 5 785.53 0.12 540 180 183 3 862.68 1.20 541 180 216 36 317.47 2.94 542 181 184 3 844.11 0.74 543 181 185 4 817.66 1.02 544 181 214 33 318.14 2.65 545 181 215 34 318.29 2.75 546 181 216 35 317.70 3.04 547 181 218 37 317.64 3.06 548 182 185 3 841.37 0.62 549 182 186 4 817.66 1.02 550 182 214 32 318.28 2.73 551 182 216 34 317.71 3.03 552 183 186 3 832.15 6.54 553 183 187 4 805.78 6.96 554 184 187 3 843.24 0.52 555 184 188 4 798.92 4.71 556 185 188 3 836.27 4.04 557 185 189 4 798.92 4.70 558 185 213 28 318.31 2.71 559 185 214 29 318.33 2.73 560 186 189 3 850.00 1.73 561 186 190 4 802.05 6.25 562 186 213 27 318.31 2.71 563 186 230 44 177.98 14.77 564 186 234 48 177.85 14.76 565 187 190 3 853.33 0.07 566 189 192 3 855.18 1.02 567 189 193 4 802.01 6.32 568 189 211 22 318.54 2.73 569 189 212 23 318.56 2.74 570 189 213 24 318.46 2.78 571 190 193 3 841.33 5.90 572 190 235 45 177.94 14.69 573 190 237 47 177.56 14.87 574 190 239 49 174.26 14.41 575 191 194 3 803.64 0.55 576 191 195 4 814.84 5.85 577 191 239 48 174.40 14.47 578 191 244 53 139.22 28.12 579 192 195 3 827.47 4.38 580 192 196 4 822.52 6.86 581 192 211 19 318.62 2.71 582 192 249 57 14.52 0.43 583 193 196 3 855.15 1.02 584 193 197 4 828.11 4.06 585 193 207 14 320.81 2.73 586 193 211 18 318.60 2.72 587 193 223 30 317.64 3.23 588 193 230 37 207.34 0.21 589 193 235 42 178.18 14.57 590 194 197 3 850.18 4.25 591 194 198 4 828.11 4.05 592 194 238 44 176.02 15.29 593 194 239 45 174.83 14.72 594 194 244 50 139.54 28.28 595 195 198 3 856.09 1.29 596 195 199 4 836.22 4.76 597 195 208 13 320.83 2.73 598 195 244 49 139.55 28.28 599 195 245 50 139.52 28.27 600 195 247 52 139.46 28.25 601 195 248 53 137.46 28.88 602 195 249 54 102.15 30.41 603 196 199 3 836.20 4.75 604 196 200 4 823.34 1.69 605 196 207 11 320.82 2.73 606 196 208 12 320.81 2.73 607 196 209 13 320.75 2.73 608 196 249 53 114.30 33.80 609 197 200 3 837.88 1.67 610 197 201 4 837.89 1.68 611 197 207 10 320.84 2.72 612 197 231 34 209.27 1.17 613 197 235 38 185.46 11.13 614 197 238 41 176.37 15.46 615 198 201 3 862.00 1.62 616 198 241 43 174.61 15.13 617 199 202 3 862.00 1.62 618 199 208 9 320.88 2.73 619 200 203 3 838.73 1.27 620 200 204 4 794.35 4.03 621 200 205 5 562.83 98.56 622 200 206 6 320.93 2.71 623 200 207 7 320.90 2.73 624 201 204 3 837.64 0.72 625 203 206 3 851.44 5.54 626 205 225 20 317.77 3.23 627 205 226 21 317.67 3.27 628 206 224 18 317.78 3.23 629 206 225 19 317.66 3.24 630 207 211 4 854.34 0.69 631 207 223 16 317.83 3.21 632 207 224 17 317.64 3.24 633 207 225 18 317.62 3.25 634 207 226 19 317.61 3.25 635 207 231 24 213.13 0.24 636 208 248 40 139.61 28.34 637 208 249 41 138.35 28.71 638 209 249 40 136.37 29.41 639 210 222 12 317.89 3.24 640 210 223 13 317.82 3.22 641 210 224 14 317.65 3.29 642 211 222 11 317.89 3.25 643 211 223 12 317.81 3.27 644 212 221 9 318.00 3.29 645 212 222 10 317.82 3.29 646 213 220 7 318.38 3.48 647 213 221 8 318.32 3.48 648 213 223 10 317.87 3.33 649 213 230 17 209.35 1.19 650 214 218 4 581.43 93.20 651 214 219 5 321.66 1.94 652 214 220 6 318.35 3.50 653 214 221 7 318.37 3.50 654 215 218 3 815.83 11.91 655 215 219 4 323.32 1.16 656 215 221 6 318.44 3.54 657 223 228 5 336.76 2.47 658 223 231 8 320.02 2.27 659 225 228 3 829.42 5.15 660 225 231 6 343.48 3.95 661 226 231 5 338.64 1.54 662 227 232 5 338.65 1.54 663 228 231 3 847.93 2.01 664 228 232 4 794.50 5.12 665 229 232 3 820.48 7.71 666 229 234 5 674.48 47.49 667 230 234 4 552.53 108.47 668 230 235 5 552.50 108.45 669 231 235 4 551.77 108.11 670 239 243 4 816.68 1.31 671 239 244 5 761.46 2.72 672 240 243 3 839.09 3.30 673 240 244 4 812.25 4.39 674 241 244 3 846.59 0.45 675 241 245 4 817.55 1.75 676 242 245 3 852.07 0.65 677 242 246 4 806.18 7.42 678 243 247 4 829.04 4.70 679 244 247 3 879.52 1.02