BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 51 50 35.43 1.76 2 2 17 15 35.03 1.79 3 2 20 18 30.96 3.46 4 2 21 19 28.66 2.33 5 2 24 22 28.73 2.37 6 2 49 47 31.18 1.15 7 2 50 48 34.40 1.78 8 2 51 49 35.63 1.67 9 3 17 14 36.70 1.50 10 3 51 48 36.01 1.85 11 4 9 5 775.69 9.18 12 4 13 9 41.68 0.26 13 4 14 10 36.88 1.53 14 4 17 13 36.75 1.49 15 4 51 47 37.04 1.59 16 4 52 48 36.97 1.49 17 4 53 49 51.63 0.38 18 4 129 125 60.16 0.10 19 4 132 128 60.01 0.03 20 5 9 4 805.88 11.26 21 5 53 48 54.64 0.01 22 5 57 52 60.65 0.33 23 6 9 3 831.50 1.43 24 6 53 47 60.12 0.08 25 6 54 48 60.16 0.10 26 6 55 49 61.06 0.26 27 6 129 123 60.17 0.10 28 6 132 126 60.17 0.10 29 6 133 127 60.19 0.10 30 6 136 130 60.91 0.19 31 7 55 48 61.63 0.43 32 9 13 4 829.74 1.31 33 9 129 120 80.28 0.12 34 9 175 166 85.63 0.02 35 10 13 3 853.46 1.93 36 10 14 4 835.50 2.49 37 10 175 165 89.91 0.86 38 11 14 3 847.98 0.93 39 11 15 4 843.59 3.12 40 11 172 161 82.20 1.08 41 11 173 162 87.89 0.71 42 11 175 164 92.67 0.42 43 12 15 3 847.98 0.93 44 12 16 4 843.59 3.13 45 13 16 3 843.59 3.13 46 13 17 4 843.58 3.12 47 14 17 3 845.09 2.27 48 14 18 4 834.40 3.06 49 14 129 115 96.12 0.38 50 14 157 143 93.28 0.13 51 14 161 147 92.67 0.43 52 14 168 154 92.67 0.43 53 14 171 157 95.59 0.55 54 14 172 158 95.44 0.62 55 15 18 3 849.74 0.05 56 15 19 4 834.37 3.07 57 15 168 153 92.91 0.33 58 16 19 3 852.25 6.73 59 16 20 4 834.21 3.02 60 17 20 3 852.24 6.73 61 17 21 4 824.42 1.12 62 17 50 33 107.88 0.36 63 17 52 35 117.16 0.54 64 17 158 141 99.03 1.39 65 18 21 3 840.51 6.17 66 18 22 4 823.87 1.50 67 18 157 139 104.14 0.69 68 18 158 140 100.11 0.95 69 18 161 143 96.42 0.24 70 18 162 144 96.12 0.37 71 18 168 150 98.86 1.45 72 19 22 3 839.86 6.50 73 19 23 4 823.82 1.52 74 20 23 3 832.28 2.70 75 20 24 4 819.34 3.77 76 21 24 3 838.21 5.66 77 21 25 4 842.14 7.63 78 21 50 29 118.93 0.26 79 21 69 48 110.99 1.18 80 21 158 137 118.20 0.04 81 21 162 141 112.64 0.24 82 22 25 3 858.38 6.67 83 22 161 139 118.24 0.04 84 22 162 140 115.37 0.06 85 22 164 142 118.14 0.06 86 22 165 143 118.23 0.05 87 22 168 146 118.62 0.16 88 22 238 216 110.88 0.18 89 22 239 217 107.95 0.34 90 25 37 12 122.71 0.49 91 25 48 23 123.58 0.28 92 25 66 41 122.63 0.48 93 25 69 44 121.95 0.80 94 25 239 214 122.39 0.46 95 26 37 11 123.44 0.36 96 26 38 12 123.59 0.42 97 26 41 15 123.92 0.34 98 26 66 40 123.58 0.28 99 27 30 3 937.59 0.33 100 27 35 8 123.82 0.33 101 27 36 9 123.67 0.33 102 27 37 10 123.44 0.36 103 27 38 11 123.92 0.39 104 28 36 8 123.94 0.39 105 28 37 9 123.94 0.39 106 28 38 10 123.95 0.38 107 28 39 11 124.59 0.60 108 28 242 214 122.57 0.07 109 29 35 6 125.70 0.40 110 29 36 7 124.97 0.42 111 30 33 3 835.89 8.04 112 30 34 4 508.30 108.53 113 30 35 5 125.75 0.39 114 31 34 3 845.90 3.03 115 31 36 5 548.11 129.50 116 31 199 168 155.68 0.95 117 31 200 169 148.14 1.75 118 31 203 172 157.59 1.07 119 31 240 209 129.55 0.03 120 32 199 167 157.67 1.05 121 32 202 170 157.78 1.05 122 32 203 171 157.72 1.07 123 32 207 175 158.05 1.03 124 32 228 196 157.93 0.97 125 34 199 165 158.36 0.96 126 34 233 199 158.47 0.96 127 34 237 203 158.47 0.96 128 34 238 204 158.34 0.96 129 34 239 205 158.35 0.96 130 34 240 206 158.31 0.96 131 35 238 203 158.50 0.96 132 35 239 204 158.36 0.96 133 35 240 205 158.35 0.96 134 36 200 164 158.41 0.95 135 36 240 204 158.37 0.96 136 36 242 206 158.12 0.95 137 37 66 29 158.55 0.95 138 37 67 30 158.52 0.94 139 37 68 31 158.52 0.94 140 37 69 32 158.53 0.94 141 37 192 155 158.58 0.96 142 37 239 202 158.58 0.95 143 37 240 203 158.42 0.94 144 37 241 204 158.36 0.96 145 37 242 205 158.31 0.96 146 38 41 3 909.67 8.93 147 38 67 29 158.56 0.95 148 38 242 204 158.35 0.96 149 38 245 207 157.86 0.94 150 39 242 203 158.34 0.94 151 39 244 205 158.28 0.94 152 39 245 206 157.86 0.94 153 40 46 6 751.97 0.18 154 40 67 27 703.18 12.97 155 41 45 4 828.59 5.33 156 41 46 5 751.97 0.18 157 41 66 25 708.77 10.20 158 41 67 26 705.23 14.00 159 42 45 3 866.80 2.94 160 43 46 3 866.80 2.93 161 43 47 4 866.80 2.93 162 43 94 51 556.35 31.35 163 44 47 3 866.80 2.93 164 44 94 50 564.12 31.74 165 45 66 21 717.21 13.61 166 46 65 19 717.22 13.61 167 46 66 20 717.19 13.60 168 46 67 21 717.19 13.60 169 47 64 17 717.25 13.60 170 47 65 18 717.23 13.60 171 47 66 19 717.21 13.60 172 47 72 25 716.38 13.69 173 47 90 43 589.95 23.57 174 47 91 44 589.61 23.41 175 47 94 47 589.14 23.23 176 47 95 48 589.11 23.23 177 48 63 15 717.36 13.63 178 48 64 16 717.26 13.60 179 48 66 18 717.23 13.61 180 49 62 13 717.44 13.62 181 49 63 14 717.36 13.63 182 49 95 46 589.82 23.51 183 49 96 47 589.65 23.43 184 50 61 11 717.66 13.69 185 50 62 12 717.47 13.61 186 50 64 14 717.47 13.61 187 50 158 108 444.79 0.53 188 51 59 8 718.37 13.50 189 51 60 9 718.07 13.59 190 51 61 10 717.66 13.70 191 52 59 7 718.37 13.49 192 52 60 8 718.29 13.48 193 52 62 10 718.03 13.62 194 52 136 84 544.18 2.01 195 52 154 102 507.72 1.99 196 52 158 106 470.44 2.23 197 53 57 4 718.80 13.56 198 53 58 5 718.50 13.45 199 53 59 6 718.38 13.49 200 53 136 83 544.36 2.05 201 54 57 3 825.20 9.25 202 54 58 4 718.78 13.56 203 54 133 79 544.29 2.09 204 54 136 82 544.36 2.05 205 54 137 83 544.43 2.01 206 55 130 75 544.45 2.08 207 55 133 78 544.40 2.06 208 58 136 78 544.43 2.02 209 58 140 82 544.43 1.98 210 58 141 83 544.47 1.97 211 59 140 81 544.42 1.98 212 60 74 14 716.43 13.75 213 60 75 15 716.39 13.75 214 60 76 16 716.16 13.64 215 60 136 76 544.23 1.99 216 60 139 79 544.33 1.93 217 60 140 80 544.24 1.89 218 60 154 94 507.79 2.04 219 61 74 13 716.38 13.71 220 61 75 14 716.31 13.69 221 61 96 35 589.97 23.55 222 62 71 9 716.77 13.79 223 62 73 11 716.45 13.71 224 62 74 12 716.31 13.69 225 62 154 92 506.57 2.36 226 63 71 8 716.76 13.79 227 63 72 9 716.53 13.68 228 63 73 10 716.38 13.69 229 63 95 32 589.70 23.45 230 63 96 33 589.69 23.45 231 64 69 5 717.05 13.66 232 64 70 6 716.80 13.77 233 64 71 7 716.57 13.75 234 65 68 3 854.21 3.97 235 65 69 4 717.75 13.28 236 65 70 5 716.62 13.72 237 65 72 7 716.38 13.68 238 67 241 174 162.41 0.84 239 68 163 95 441.66 0.63 240 68 192 124 282.67 1.35 241 68 241 173 170.94 3.43 242 69 159 90 443.80 0.95 243 69 162 93 441.87 0.67 244 69 163 94 441.69 0.58 245 69 192 123 282.68 1.34 246 69 239 170 191.26 0.55 247 70 163 93 441.87 0.67 248 71 83 12 590.67 23.56 249 71 154 83 502.12 0.25 250 71 155 84 480.24 2.52 251 71 158 87 443.98 0.95 252 72 82 10 590.68 23.56 253 72 83 11 590.64 23.55 254 72 87 15 590.31 23.48 255 72 88 16 590.08 23.54 256 72 91 19 590.01 23.57 257 73 81 8 590.75 23.57 258 73 82 9 590.68 23.56 259 74 80 6 591.10 23.49 260 74 81 7 590.90 23.57 261 74 96 22 589.98 23.55 262 74 139 65 544.23 1.92 263 74 142 68 544.22 1.85 264 74 151 77 541.32 1.60 265 75 78 3 898.57 4.84 266 75 79 4 816.34 4.04 267 75 80 5 591.11 23.49 268 75 96 21 589.98 23.55 269 75 99 24 589.14 23.25 270 76 79 3 851.74 0.74 271 76 81 5 809.22 0.48 272 76 140 64 544.16 1.88 273 78 99 21 589.17 23.24 274 78 100 22 589.14 23.25 275 78 101 23 589.12 23.25 276 79 101 22 588.72 23.39 277 79 103 24 588.31 23.51 278 80 96 16 590.00 23.57 279 80 99 19 589.13 23.25 280 80 101 21 588.64 23.35 281 80 102 22 587.42 23.09 282 80 103 23 584.92 21.88 283 81 103 22 564.31 11.62 284 81 105 24 546.15 2.67 285 81 142 61 544.14 1.81 286 82 88 6 590.51 23.50 287 82 91 9 590.51 23.50 288 82 92 10 590.37 23.49 289 82 95 13 590.27 23.56 290 82 96 14 590.15 23.52 291 82 103 21 564.14 11.58 292 82 104 22 546.15 2.67 293 82 105 23 546.11 2.68 294 83 88 5 703.02 16.86 295 83 105 22 546.06 2.69 296 83 107 24 544.45 3.06 297 83 108 25 544.32 3.06 298 83 109 26 544.32 3.08 299 83 114 31 544.38 3.04 300 83 151 68 542.01 1.83 301 83 155 72 508.19 1.90 302 84 104 20 546.15 2.67 303 84 105 21 546.10 2.67 304 84 107 23 544.46 3.06 305 84 108 24 544.45 3.05 306 85 88 3 857.94 8.54 307 85 89 4 839.10 0.41 308 85 108 23 544.36 3.10 309 85 109 24 544.36 3.10 310 86 89 3 839.53 0.20 311 86 90 4 839.11 0.41 312 87 90 3 843.66 1.86 313 87 91 4 839.10 0.42 314 88 91 3 841.99 1.02 315 88 92 4 841.88 0.98 316 89 92 3 842.08 1.11 317 89 93 4 841.65 1.32 318 90 93 3 862.37 7.40 319 90 94 4 842.09 1.11 320 91 94 3 858.51 9.33 321 91 95 4 840.83 1.50 322 93 97 4 838.03 1.69 323 94 97 3 878.75 6.63 324 96 99 3 860.43 2.04 325 96 102 6 708.02 9.01 326 97 102 5 816.83 2.02 327 99 102 3 889.51 2.33 328 105 142 37 563.62 7.89 329 105 151 46 561.45 7.70 330 106 142 36 563.87 7.91 331 107 113 6 726.01 6.53 332 107 114 7 630.14 37.97 333 107 117 10 569.49 9.60 334 107 147 40 563.57 8.12 335 107 148 41 563.41 8.15 336 107 151 44 563.03 8.35 337 108 113 5 785.44 6.67 338 108 114 6 785.59 6.58 339 109 114 5 799.06 5.38 340 110 113 3 834.55 3.48 341 110 114 4 820.53 0.96 342 111 114 3 830.65 5.78 343 111 115 4 820.58 0.95 344 111 122 11 568.54 9.78 345 111 152 41 563.11 8.43 346 111 155 44 562.57 8.69 347 111 156 45 562.51 8.70 348 111 160 49 541.95 17.89 349 111 189 78 298.50 4.21 350 112 115 3 842.09 1.03 351 112 116 4 820.58 0.93 352 113 116 3 844.68 1.39 353 113 117 4 821.01 0.71 354 114 117 3 848.03 0.29 355 114 118 4 823.63 1.82 356 114 148 34 565.47 9.12 357 114 151 37 565.08 9.30 358 114 152 38 563.63 8.71 359 114 155 41 563.13 8.96 360 115 118 3 841.95 3.34 361 115 119 4 823.64 1.82 362 115 120 5 770.99 1.85 363 115 121 6 678.74 13.91 364 115 122 7 678.74 13.91 365 115 152 37 565.21 9.36 366 116 119 3 831.08 1.90 367 117 148 31 565.65 9.16 368 118 148 30 565.66 9.16 369 118 149 31 565.63 9.17 370 118 152 34 565.36 9.31 371 120 124 4 820.35 2.06 372 120 125 5 787.06 5.80 373 121 124 3 842.88 7.86 374 121 125 4 825.82 0.67 375 122 125 3 842.87 7.86 376 122 152 30 566.02 9.38 377 122 156 34 565.19 9.48 378 122 181 59 424.55 1.05 379 122 186 64 356.22 8.64 380 122 189 67 349.07 12.18 381 122 190 68 349.66 8.64 382 123 180 57 424.78 1.02 383 123 181 58 424.62 1.07 384 123 183 60 423.41 1.11 385 123 186 63 375.33 3.54 386 124 180 56 424.82 1.03 387 125 152 27 566.04 9.39 388 125 153 28 565.68 9.58 389 125 180 55 424.82 1.03 390 125 181 56 424.80 1.03 391 126 131 5 797.52 5.82 392 126 153 27 565.75 9.62 393 126 178 52 425.00 1.01 394 126 179 53 424.97 0.99 395 126 180 54 424.81 1.02 396 127 131 4 813.99 7.38 397 127 153 26 565.62 9.70 398 127 156 29 565.04 9.93 399 127 171 44 441.42 0.61 400 127 178 51 424.98 0.99 401 127 179 52 424.97 0.99 402 127 181 54 423.83 0.70 403 128 131 3 848.59 0.33 404 128 132 4 831.68 8.13 405 128 171 43 441.39 0.62 406 128 174 46 434.05 1.16 407 128 179 51 424.98 0.99 408 129 132 3 832.10 7.92 409 129 133 4 822.11 3.33 410 129 171 42 441.39 0.63 411 129 174 45 434.05 1.16 412 129 175 46 428.14 1.36 413 130 133 3 829.19 6.46 414 130 134 4 822.12 3.31 415 130 174 44 436.41 0.02 416 130 175 45 431.01 0.01 417 130 176 46 427.49 1.11 418 130 177 47 426.31 0.95 419 130 178 48 425.05 1.01 420 131 134 3 838.05 4.66 421 131 135 4 822.10 3.29 422 131 153 22 565.48 9.91 423 131 178 47 424.99 0.99 424 132 135 3 837.99 4.69 425 132 136 4 834.38 2.89 426 132 153 21 565.06 10.12 427 132 154 22 564.84 10.23 428 132 157 25 548.12 18.39 429 133 136 3 845.63 0.87 430 133 137 4 834.37 2.89 431 134 137 3 839.93 0.11 432 134 138 4 829.29 0.36 433 135 138 3 847.28 6.44 434 135 139 4 825.01 1.10 435 135 150 15 565.12 10.11 436 135 153 18 564.92 10.21 437 136 139 3 853.56 2.92 438 136 140 4 820.43 1.33 439 137 140 3 831.58 0.24 440 137 141 4 821.39 0.84 441 138 141 3 847.75 3.98 442 138 142 4 820.11 1.32 443 138 143 5 767.90 5.98 444 138 146 8 567.05 9.78 445 138 147 9 566.65 9.71 446 138 150 12 566.11 9.96 447 139 142 3 820.00 1.40 448 139 147 8 566.67 9.74 449 139 150 11 565.89 10.11 450 139 151 12 565.86 10.11 451 139 154 15 548.94 18.54 452 142 147 5 771.65 0.95 453 143 146 3 852.23 5.33 454 143 147 4 806.03 1.20 455 144 147 3 842.85 1.58 456 146 149 3 855.85 0.13 457 146 150 4 818.16 0.33 458 147 150 3 832.59 7.54 459 147 151 4 817.09 0.87 460 148 151 3 831.60 6.25 461 148 152 4 828.03 4.46 462 149 152 3 831.43 5.88 463 149 153 4 828.03 4.46 464 150 153 3 833.78 1.23 465 150 154 4 828.25 3.99 466 151 154 3 833.82 1.21 467 151 155 4 820.01 0.17 468 152 155 3 839.01 1.39 469 152 156 4 825.55 2.93 470 153 156 3 840.83 0.48 471 153 157 4 820.01 0.16 472 154 157 3 835.69 2.14 473 154 158 4 835.63 2.11 474 155 158 3 856.86 0.76 475 155 159 4 815.11 2.56 476 155 160 5 793.35 8.32 477 156 160 4 782.13 6.99 478 156 161 5 787.86 4.12 479 156 171 15 441.40 0.60 480 156 181 25 405.81 0.31 481 156 189 33 298.35 4.19 482 157 161 4 824.81 3.32 483 157 171 14 441.33 0.61 484 158 161 3 844.37 1.72 485 158 162 4 830.07 0.70 486 159 162 3 830.08 0.69 487 159 163 4 830.08 0.70 488 160 163 3 840.42 3.54 489 160 164 4 831.00 1.17 490 160 167 7 527.20 43.43 491 160 189 29 298.30 4.17 492 161 164 3 840.05 4.08 493 161 167 6 515.27 37.47 494 161 168 7 441.45 0.59 495 161 171 10 441.42 0.63 496 162 238 76 192.19 0.58 497 162 239 77 191.43 0.56 498 163 192 29 283.88 0.99 499 163 237 74 192.36 0.51 500 163 238 75 192.21 0.57 501 163 239 76 191.40 0.57 502 164 167 3 845.77 1.02 503 164 168 4 831.48 2.31 504 164 188 24 298.58 4.08 505 164 189 25 298.61 4.09 506 164 192 28 296.31 5.08 507 164 195 31 285.56 0.23 508 164 236 72 192.52 0.56 509 164 237 73 192.37 0.52 510 165 168 3 840.36 1.69 511 165 169 4 825.34 5.38 512 165 235 70 192.70 0.52 513 165 236 71 192.53 0.56 514 166 169 3 846.00 4.95 515 166 170 4 826.72 4.68 516 166 185 19 346.28 13.62 517 166 188 22 298.88 4.17 518 166 236 70 192.60 0.57 519 167 170 3 847.61 4.14 520 167 171 4 835.56 1.01 521 167 185 18 313.03 2.98 522 167 188 21 298.74 4.15 523 167 189 22 298.72 4.12 524 168 171 3 853.45 3.06 525 168 172 4 834.83 1.36 526 169 172 3 849.80 3.04 527 169 173 4 834.86 1.35 528 170 173 3 863.01 1.92 529 170 174 4 846.42 2.47 530 170 179 9 425.00 0.98 531 171 174 3 863.01 1.92 532 171 179 8 424.97 0.99 533 172 175 3 883.81 0.59 534 174 177 3 847.38 0.32 535 174 178 4 770.36 1.44 536 174 179 5 743.19 15.03 537 181 185 4 805.10 6.66 538 181 186 5 805.30 6.75 539 182 185 3 836.49 2.27 540 182 186 4 823.62 2.36 541 183 186 3 833.92 1.26 542 183 187 4 832.30 2.08 543 183 216 33 254.44 1.95 544 183 220 37 254.39 1.92 545 184 187 3 846.10 4.79 546 184 188 4 834.07 1.54 547 184 216 32 254.44 1.96 548 184 217 33 254.38 1.92 549 184 218 34 254.50 1.98 550 185 188 3 867.19 5.76 551 185 189 4 846.44 4.62 552 186 189 3 851.89 1.89 553 186 190 4 846.45 4.61 554 187 190 3 846.47 4.61 555 187 191 4 846.46 4.61 556 187 215 28 257.35 2.49 557 187 216 29 257.52 2.41 558 188 191 3 851.88 1.91 559 188 192 4 837.51 0.14 560 188 215 27 258.04 2.50 561 188 232 44 211.87 2.25 562 188 236 48 211.38 2.50 563 189 192 3 858.45 1.39 564 191 194 3 840.27 5.14 565 191 195 4 830.37 1.26 566 191 213 22 259.35 2.12 567 191 214 23 260.25 2.58 568 191 215 24 259.35 2.12 569 192 195 3 847.24 8.62 570 192 196 4 831.65 1.90 571 192 237 45 211.38 2.50 572 192 239 47 211.35 2.50 573 192 240 48 210.84 2.27 574 192 241 49 208.36 2.95 575 193 196 3 836.08 0.30 576 193 197 4 831.69 1.89 577 193 241 48 208.36 2.95 578 193 246 53 166.70 0.35 579 194 197 3 840.16 2.35 580 194 198 4 831.77 1.84 581 194 213 19 261.33 2.79 582 195 198 3 849.61 2.38 583 195 199 4 827.12 2.94 584 195 209 14 262.14 3.17 585 195 213 18 261.36 2.79 586 195 215 20 261.38 2.78 587 195 225 30 261.24 2.84 588 195 232 37 228.93 1.50 589 195 237 42 211.41 2.48 590 196 199 3 852.77 1.12 591 196 200 4 832.17 0.42 592 196 240 44 210.88 2.27 593 196 241 45 208.93 2.67 594 196 246 50 190.52 2.44 595 197 200 3 845.01 3.32 596 197 201 4 826.64 3.17 597 197 246 49 188.80 0.79 598 198 201 3 832.11 1.45 599 198 202 4 826.47 3.15 600 198 209 11 262.14 3.18 601 198 210 12 262.24 3.12 602 198 211 13 262.19 3.10 603 198 213 15 261.92 3.03 604 199 202 3 832.56 0.10 605 199 203 4 826.57 3.25 606 199 209 10 262.20 3.15 607 199 233 34 238.11 1.33 608 199 237 38 212.05 2.42 609 199 240 41 211.46 2.51 610 200 203 3 860.66 3.74 611 200 240 40 211.47 2.51 612 200 241 41 211.46 2.52 613 200 242 42 211.36 2.48 614 200 243 43 210.13 2.10 615 201 204 3 863.56 2.29 616 201 205 4 826.20 3.06 617 201 210 9 262.36 3.08 618 201 243 42 210.97 2.52 619 202 205 3 824.86 4.10 620 202 206 4 818.26 7.02 621 202 207 5 280.07 12.02 622 202 208 6 263.01 3.48 623 202 209 7 263.01 3.48 624 202 228 26 261.47 2.87 625 205 208 3 833.32 0.35 626 207 227 20 261.83 2.80 627 207 228 21 261.75 2.81 628 208 226 18 262.13 2.68 629 208 227 19 261.93 2.72 630 209 213 4 855.87 0.56 631 209 225 16 262.47 2.51 632 209 226 17 262.11 2.66 633 209 227 18 262.07 2.65 634 209 228 19 262.10 2.66 635 209 233 24 247.63 4.58 636 212 224 12 263.14 2.23 637 212 225 13 262.78 2.40 638 212 226 14 262.62 2.41 639 213 224 11 263.25 2.29 640 213 225 12 262.98 2.32 641 214 222 8 264.75 2.50 642 214 223 9 263.80 2.06 643 214 224 10 263.27 2.30 644 215 222 7 264.75 2.50 645 215 223 8 264.50 2.41 646 215 225 10 263.75 2.06 647 215 232 17 241.35 8.34 648 216 220 4 540.35 122.70 649 216 221 5 278.30 3.60 650 216 222 6 264.85 2.46 651 217 220 3 818.31 6.23 652 217 221 4 303.60 3.27 653 217 223 6 265.53 2.77 654 225 230 5 463.85 69.33 655 225 232 7 462.73 70.02 656 225 233 8 462.60 69.95 657 227 230 3 870.28 6.02 658 227 233 6 463.12 69.84 659 228 233 5 463.12 69.84 660 228 234 6 499.36 82.51 661 229 234 5 499.37 82.50 662 230 233 3 831.37 0.90 663 230 234 4 813.55 0.35 664 231 234 3 816.84 1.29 665 231 235 4 630.80 91.01 666 231 236 5 555.91 54.30 667 232 236 4 558.12 55.40 668 232 237 5 499.43 82.53 669 233 237 4 650.54 32.88 670 237 240 3 933.77 3.68 671 241 245 4 833.94 0.07 672 241 246 5 779.41 0.03 673 242 245 3 834.89 0.41 674 242 246 4 834.90 0.41 675 243 246 3 824.44 5.64