BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 247 245 18.81 0.26 2 2 248 246 18.77 0.24 3 3 248 245 22.98 0.69 4 3 249 246 20.96 0.79 5 3 250 247 18.96 0.34 6 4 248 244 26.66 0.12 7 4 249 245 24.63 1.14 8 5 116 111 45.30 0.43 9 5 244 239 43.73 1.09 10 5 247 242 34.31 0.14 11 5 248 243 29.64 0.65 12 6 117 111 45.34 0.42 13 6 118 112 42.36 0.29 14 6 247 241 38.52 0.01 15 6 248 242 32.30 0.62 16 8 12 4 849.60 4.66 17 8 13 5 840.19 0.04 18 8 117 109 194.78 75.12 19 8 118 110 180.72 68.11 20 9 12 3 865.50 3.30 21 9 13 4 853.83 5.28 22 10 13 3 858.38 3.00 23 10 14 4 855.20 4.59 24 11 14 3 855.55 4.44 25 11 15 4 858.83 2.79 26 11 90 79 560.24 21.14 27 12 15 3 870.58 3.07 28 12 16 4 859.14 2.66 29 12 90 78 560.24 21.15 30 12 91 79 559.76 20.93 31 12 92 80 559.78 20.93 32 12 95 83 559.77 20.91 33 12 117 105 513.77 2.10 34 13 16 3 884.59 15.37 35 13 17 4 874.63 4.96 36 14 17 3 874.64 4.96 37 14 18 4 874.63 4.96 38 15 18 3 880.61 2.00 39 15 19 4 874.65 4.98 40 15 88 73 569.66 18.18 41 15 90 75 569.78 18.23 42 15 91 76 569.65 18.18 43 16 19 3 874.74 5.01 44 16 20 4 874.66 4.98 45 16 91 75 569.86 18.28 46 16 95 79 569.65 18.18 47 16 99 83 569.18 17.93 48 16 115 99 533.66 0.08 49 16 117 101 533.49 0.07 50 17 20 3 889.67 1.03 51 17 21 4 874.67 5.01 52 18 21 3 886.15 0.73 53 18 22 4 874.65 5.02 54 18 88 70 572.26 18.41 55 19 22 3 872.19 7.50 56 19 23 4 868.62 8.04 57 19 88 69 571.53 18.05 58 19 99 80 570.92 17.76 59 20 23 3 872.19 7.50 60 20 24 4 858.08 0.46 61 20 99 79 570.94 17.77 62 20 103 83 585.48 10.44 63 20 106 86 584.95 8.09 64 21 24 3 871.89 5.72 65 21 25 4 866.51 4.05 66 22 25 3 891.70 0.12 67 22 26 4 859.98 2.70 68 22 84 62 588.37 10.59 69 22 87 65 588.38 10.59 70 22 88 66 588.38 10.58 71 23 26 3 891.18 0.14 72 23 27 4 859.98 2.70 73 23 81 58 588.38 10.59 74 23 84 61 588.39 10.58 75 23 96 73 587.44 10.11 76 23 99 76 587.15 9.96 77 23 100 77 586.95 9.87 78 23 103 80 587.54 9.49 79 24 27 3 891.18 0.13 80 24 28 4 852.55 0.26 81 24 103 79 587.59 9.47 82 24 106 82 588.00 9.17 83 24 107 83 578.32 4.32 84 25 28 3 874.02 8.45 85 25 29 4 852.55 0.27 86 26 29 3 871.87 0.09 87 26 30 4 849.60 1.15 88 26 80 54 589.23 10.31 89 26 81 55 589.11 10.26 90 26 84 58 589.10 10.25 91 27 30 3 865.59 3.23 92 27 31 4 852.02 0.13 93 27 32 5 796.24 2.44 94 27 33 6 738.87 2.64 95 27 36 9 729.85 1.88 96 27 100 73 589.28 9.88 97 27 103 76 588.34 9.38 98 27 104 77 588.14 9.21 99 27 107 80 585.61 7.80 100 28 31 3 861.93 0.33 101 28 107 79 585.53 7.83 102 31 184 153 246.40 28.80 103 32 35 3 886.01 0.78 104 32 36 4 856.20 2.52 105 32 80 48 606.64 3.83 106 33 36 3 870.49 2.12 107 33 37 4 855.55 2.85 108 33 104 71 601.66 2.73 109 33 108 75 601.71 2.25 110 34 37 3 871.88 0.79 111 34 38 4 851.15 1.33 112 34 165 131 258.80 23.18 113 34 166 132 258.70 23.12 114 34 183 149 260.06 22.25 115 34 184 150 258.76 22.89 116 35 38 3 872.90 3.35 117 35 39 4 857.35 4.42 118 35 73 38 623.40 5.19 119 35 74 39 623.37 5.19 120 35 76 41 623.32 5.21 121 35 77 42 623.06 5.32 122 35 80 45 623.00 5.35 123 35 172 137 261.80 22.39 124 36 39 3 872.90 3.35 125 36 40 4 863.38 1.41 126 36 77 41 623.06 5.32 127 36 80 44 622.89 5.39 128 36 81 45 622.87 5.40 129 36 100 64 604.43 2.83 130 36 101 65 604.39 2.83 131 36 104 68 602.46 2.40 132 37 40 3 870.38 2.08 133 37 41 4 870.13 1.97 134 37 101 64 604.38 2.84 135 37 104 67 602.59 2.37 136 37 105 68 602.51 2.40 137 37 162 125 261.09 22.44 138 37 165 128 260.73 22.25 139 37 235 198 199.62 1.39 140 37 239 202 195.25 1.28 141 38 41 3 870.15 1.97 142 38 42 4 858.96 0.82 143 38 70 32 624.22 5.32 144 38 71 33 624.21 5.33 145 38 72 34 624.17 5.33 146 38 74 36 623.39 5.19 147 38 172 134 261.92 22.38 148 39 42 3 866.06 4.25 149 39 43 4 858.96 0.82 150 39 60 21 624.17 5.30 151 39 74 35 623.40 5.20 152 39 77 38 623.17 5.26 153 39 78 39 623.16 5.26 154 39 81 42 623.24 5.21 155 39 97 58 606.72 3.21 156 40 43 3 859.49 1.08 157 40 44 4 860.21 1.44 158 40 97 57 606.71 3.21 159 40 98 58 606.64 3.18 160 40 101 61 604.48 2.81 161 41 44 3 882.39 6.46 162 41 45 4 858.16 2.46 163 41 131 90 482.77 3.71 164 41 132 91 482.74 3.70 165 41 145 104 265.99 23.49 166 41 149 108 266.14 23.53 167 41 242 201 188.38 0.29 168 42 46 4 874.34 4.74 169 42 60 18 624.25 5.28 170 42 70 28 624.25 5.32 171 42 71 29 624.20 5.31 172 42 74 32 624.13 5.31 173 42 131 89 482.76 3.71 174 43 46 3 915.60 2.72 175 43 55 12 625.43 4.97 176 43 56 13 625.38 4.98 177 43 60 17 624.25 5.28 178 43 98 55 606.90 3.27 179 44 47 3 918.56 1.18 180 44 55 11 625.39 4.98 181 44 56 12 625.37 4.97 182 44 98 54 607.00 3.31 183 44 242 198 188.38 0.29 184 44 243 199 188.33 0.29 185 44 245 201 187.22 0.53 186 45 131 86 482.76 3.71 187 45 149 104 265.99 23.48 188 45 242 197 188.40 0.29 189 46 53 7 633.75 6.94 190 46 54 8 627.48 4.11 191 46 55 9 627.43 4.12 192 46 126 80 555.48 1.10 193 46 130 84 485.31 4.79 194 46 131 85 483.09 3.77 195 47 52 5 687.92 14.29 196 47 53 6 633.70 6.94 197 47 54 7 628.40 4.57 198 47 55 8 627.46 4.11 199 47 148 101 267.45 23.20 200 47 149 102 267.05 23.31 201 47 245 198 188.21 0.32 202 47 246 199 187.22 0.53 203 48 51 3 880.44 6.52 204 48 52 4 742.05 5.67 205 48 53 5 635.31 7.71 206 48 130 82 525.67 7.08 207 48 148 100 267.45 23.20 208 49 52 3 838.71 1.55 209 49 147 98 267.64 23.14 210 49 148 99 267.61 23.15 211 51 130 79 555.66 1.10 212 53 130 77 554.82 0.78 213 54 57 3 959.71 2.90 214 54 126 72 555.49 1.11 215 54 130 76 520.74 9.18 216 56 94 38 607.28 3.23 217 56 98 42 607.08 3.30 218 56 119 63 579.74 8.52 219 56 243 187 188.35 0.29 220 56 245 189 187.49 0.40 221 57 94 37 607.29 3.23 222 57 119 62 579.69 8.54 223 57 120 63 579.54 8.59 224 57 121 64 579.18 8.56 225 57 122 65 578.00 8.12 226 57 245 188 188.14 0.25 227 57 246 189 187.21 0.53 228 57 247 190 186.33 0.49 229 58 61 3 914.87 3.52 230 58 93 35 607.29 3.23 231 58 94 36 607.28 3.23 232 58 121 63 579.03 8.63 233 59 62 3 897.25 5.48 234 59 65 6 796.48 6.36 235 59 122 63 577.95 8.11 236 59 124 65 563.31 1.54 237 59 125 66 556.04 1.20 238 59 126 67 556.04 1.20 239 60 65 5 798.09 7.16 240 60 66 6 796.48 6.36 241 60 71 11 640.50 3.18 242 60 74 14 624.91 5.05 243 60 97 37 607.09 3.27 244 61 66 5 837.01 11.76 245 61 78 17 623.58 5.11 246 61 93 32 607.30 3.23 247 61 97 36 607.20 3.21 248 62 65 3 879.05 6.69 249 62 66 4 862.12 0.68 250 62 123 61 573.37 5.83 251 62 125 63 557.81 1.62 252 63 66 3 862.12 0.68 253 63 67 4 862.12 0.68 254 64 67 3 889.68 1.38 255 64 68 4 878.31 7.05 256 64 125 61 563.15 1.49 257 65 68 3 881.23 5.59 258 65 71 6 802.63 4.98 259 65 125 60 558.62 2.17 260 65 126 61 557.66 1.69 261 66 71 5 706.51 9.58 262 66 74 8 626.39 4.46 263 66 75 9 625.17 5.04 264 66 78 12 625.17 5.04 265 68 71 3 875.23 4.96 266 68 72 4 864.12 1.74 267 68 126 58 561.51 0.74 268 68 127 59 556.02 1.23 269 68 128 60 545.36 2.63 270 69 72 3 880.77 2.19 271 69 128 59 545.92 2.91 272 70 131 61 496.51 0.73 273 71 74 3 874.74 3.69 274 71 131 60 496.62 0.78 275 72 172 100 261.36 22.58 276 73 76 3 897.95 2.39 277 73 172 99 261.36 22.58 278 74 77 3 861.71 2.02 279 74 78 4 856.21 0.24 280 75 78 3 870.98 3.79 281 75 79 4 856.21 0.23 282 76 79 3 870.01 1.31 283 76 80 4 858.64 0.72 284 76 172 96 261.38 22.57 285 77 80 3 871.71 2.33 286 77 81 4 868.22 4.07 287 78 81 3 892.32 1.31 288 78 82 4 868.24 4.06 289 78 97 19 606.90 3.27 290 79 82 3 891.70 2.36 291 79 83 4 888.74 3.83 292 80 83 3 929.37 1.28 293 81 84 3 882.28 0.61 294 81 85 4 871.40 2.39 295 81 96 15 607.14 3.16 296 81 97 16 606.93 3.27 297 81 100 19 605.94 2.96 298 82 85 3 868.49 0.93 299 82 86 4 871.40 2.39 300 82 93 11 618.83 8.93 301 83 86 3 873.81 2.58 302 83 87 4 870.96 4.01 303 84 87 3 895.83 0.74 304 84 88 4 870.96 4.01 305 84 96 12 618.79 8.95 306 85 88 3 882.80 1.16 307 85 89 4 872.49 3.24 308 85 91 6 817.48 3.81 309 85 92 7 766.96 9.92 310 85 93 8 657.57 14.22 311 85 96 11 607.15 3.16 312 86 89 3 887.83 4.76 313 88 91 3 886.80 2.60 314 91 95 4 858.47 3.77 315 91 96 5 842.14 4.37 316 92 95 3 860.36 4.71 317 92 96 4 844.87 3.03 318 92 119 27 579.57 8.65 319 92 121 29 579.41 8.68 320 93 96 3 858.41 3.75 321 93 97 4 851.01 0.07 322 93 121 28 579.02 8.64 323 94 97 3 881.93 0.97 324 94 98 4 858.38 3.75 325 94 119 25 579.53 8.63 326 94 120 26 579.49 8.64 327 94 121 27 579.01 8.64 328 95 98 3 881.93 0.97 329 95 99 4 858.36 3.76 330 95 115 20 580.03 8.63 331 95 117 22 579.79 8.58 332 95 118 23 579.76 8.58 333 95 119 24 579.50 8.65 334 96 99 3 875.56 2.22 335 96 100 4 860.80 5.16 336 97 100 3 868.15 1.49 337 97 101 4 858.79 6.16 338 98 101 3 863.56 7.07 339 98 102 4 855.45 1.19 340 98 115 17 580.09 8.60 341 98 119 21 579.70 8.55 342 98 243 145 188.31 0.29 343 99 102 3 882.49 0.67 344 99 103 4 855.09 1.01 345 99 115 16 580.01 8.63 346 100 103 3 861.63 1.50 347 100 104 4 855.09 1.01 348 101 104 3 860.30 2.29 349 101 105 4 856.41 0.35 350 102 105 3 879.95 1.16 351 102 106 4 856.41 0.35 352 102 113 11 580.16 8.58 353 102 239 137 195.45 1.21 354 103 106 3 856.52 0.40 355 103 107 4 853.37 1.17 356 104 108 4 846.06 1.76 357 105 108 3 883.75 6.90 358 105 109 4 844.07 2.77 359 105 110 5 720.77 1.77 360 105 111 6 743.33 9.53 361 105 186 81 260.05 21.88 362 105 235 130 199.75 1.44 363 105 239 134 195.46 1.21 364 106 109 3 863.78 4.11 365 106 110 4 807.02 3.08 366 106 112 6 667.66 18.95 367 108 165 57 260.69 22.14 368 108 184 76 260.18 21.91 369 108 185 77 260.12 21.88 370 108 186 78 260.03 21.89 371 109 186 77 260.09 21.87 372 109 232 123 216.45 0.14 373 110 186 76 260.08 21.87 374 110 232 122 215.56 0.01 375 111 186 75 260.12 21.86 376 111 232 121 215.56 0.01 377 111 235 124 200.27 1.62 378 111 236 125 199.61 1.45 379 113 236 123 198.85 1.20 380 113 239 126 195.56 1.21 381 113 240 127 195.49 1.20 382 114 240 126 195.50 1.20 383 116 240 124 195.54 1.22 384 116 244 128 188.56 0.37 385 116 247 131 187.47 0.10 386 117 247 130 187.81 0.25 387 118 247 129 187.69 0.21 388 119 243 124 188.68 0.42 389 119 244 125 188.34 0.29 390 119 247 128 185.76 0.21 391 126 130 4 910.35 2.23 392 127 130 3 981.10 0.65 393 130 144 14 267.77 23.09 394 132 140 8 269.12 22.87 395 132 144 12 268.28 22.84 396 132 145 13 268.01 23.02 397 133 136 3 872.53 1.64 398 133 140 7 525.91 88.75 399 134 175 41 265.52 21.00 400 134 205 71 236.93 6.75 401 134 206 72 236.81 6.67 402 134 207 73 236.74 6.67 403 135 175 40 265.57 21.00 404 135 206 71 236.86 6.65 405 136 140 4 853.02 1.16 406 136 207 71 237.16 6.50 407 137 140 3 873.76 2.22 408 137 141 4 862.33 3.50 409 137 210 73 237.52 6.21 410 138 141 3 869.96 0.29 411 138 142 4 862.38 3.50 412 138 210 72 238.07 5.92 413 138 214 76 236.84 5.30 414 138 217 79 230.75 2.25 415 139 142 3 892.58 4.28 416 139 143 4 862.38 3.50 417 140 143 3 884.65 8.24 418 140 144 4 862.38 3.49 419 141 144 3 881.52 9.80 420 141 145 4 850.76 2.31 421 141 146 5 842.86 6.26 422 141 156 15 274.26 20.00 423 141 207 66 242.18 3.96 424 141 211 70 242.09 3.91 425 142 146 4 823.53 3.23 426 142 147 5 823.52 3.23 427 142 153 11 284.79 25.24 428 142 156 14 283.89 24.78 429 142 214 72 239.72 2.68 430 142 216 74 238.19 1.92 431 142 217 75 234.19 0.07 432 143 147 4 747.82 4.45 433 143 148 5 722.56 8.18 434 144 148 4 722.54 8.18 435 144 149 5 722.54 8.18 436 145 149 4 722.39 8.12 437 145 150 5 722.39 8.12 438 145 151 6 430.40 98.02 439 145 156 11 283.94 24.80 440 145 159 14 283.94 24.80 441 146 150 4 722.33 8.14 442 146 151 5 514.71 58.95 443 149 242 93 195.22 1.29 444 149 245 96 188.37 0.29 445 151 155 4 859.87 1.79 446 151 159 8 822.23 9.08 447 151 241 90 195.41 1.35 448 151 242 91 195.32 1.32 449 151 245 94 193.94 1.29 450 152 155 3 863.68 0.10 451 152 156 4 865.40 0.77 452 153 156 3 876.28 6.04 453 153 157 4 865.40 0.77 454 153 216 63 258.45 15.67 455 153 217 64 258.45 15.67 456 154 157 3 881.27 3.55 457 154 158 4 863.65 0.10 458 154 220 66 258.02 15.88 459 154 224 70 257.81 15.99 460 154 234 80 214.36 0.54 461 154 237 83 212.62 0.15 462 155 158 3 865.39 0.77 463 155 159 4 863.47 0.20 464 155 238 83 196.92 0.96 465 155 241 86 195.56 1.28 466 156 159 3 878.26 1.53 467 156 160 4 855.52 4.02 468 156 211 55 270.29 10.28 469 156 217 61 258.88 15.91 470 157 160 3 865.50 0.83 471 157 161 4 869.58 0.93 472 157 191 34 323.68 10.48 473 157 211 54 270.24 10.29 474 157 217 60 258.88 15.91 475 157 220 63 258.43 16.08 476 157 221 64 257.57 16.43 477 157 224 67 256.68 16.88 478 158 161 3 874.75 3.50 479 158 224 66 256.62 16.83 480 158 231 73 223.48 3.36 481 158 234 76 213.13 0.10 482 158 235 77 204.27 1.76 483 158 238 80 196.92 0.96 484 159 162 3 879.85 0.95 485 159 235 76 200.32 1.22 486 159 238 79 196.65 1.00 487 159 239 80 195.56 1.26 488 159 242 83 191.56 0.47 489 160 163 3 900.95 0.01 490 160 190 30 333.41 15.29 491 160 211 51 270.36 10.36 492 161 164 3 889.57 4.11 493 161 190 29 323.74 10.46 494 161 191 30 323.74 10.48 495 161 231 70 227.59 5.41 496 162 165 3 901.81 0.71 497 162 186 24 346.95 21.35 498 162 235 73 209.84 6.47 499 163 179 16 307.44 0.47 500 163 190 27 344.08 20.62 501 163 205 42 274.25 9.06 502 163 208 45 271.17 10.57 503 164 180 16 345.35 20.25 504 164 183 19 347.25 21.36 505 164 186 22 347.10 21.37 506 164 187 23 346.54 21.23 507 164 190 26 340.80 22.26 508 165 183 18 347.25 21.36 509 165 184 19 347.26 21.37 510 165 185 20 347.25 21.37 511 165 186 21 347.00 21.46 512 166 178 12 349.39 20.47 513 166 179 13 349.37 20.48 514 166 183 17 347.29 21.36 515 167 177 10 349.41 20.48 516 167 178 11 349.39 20.47 517 167 179 12 349.39 20.47 518 168 173 5 803.32 2.18 519 168 175 7 365.74 12.43 520 168 176 8 353.08 18.75 521 168 177 9 352.99 18.71 522 168 179 11 349.42 20.48 523 168 203 35 323.05 32.81 524 169 172 3 865.23 1.15 525 169 173 4 829.99 4.70 526 169 174 5 764.57 21.55 527 169 175 6 525.16 66.92 528 169 176 7 366.00 12.30 529 170 173 3 872.71 2.59 530 170 174 4 659.51 0.25 531 170 175 5 525.17 66.92 532 170 176 6 525.06 66.89 533 175 203 28 323.08 32.83 534 175 205 30 322.61 32.98 535 175 206 31 322.62 32.98 536 176 203 27 323.07 32.83 537 177 203 26 323.05 32.81 538 178 183 5 521.09 65.44 539 178 201 23 323.13 32.81 540 178 202 24 323.06 32.81 541 178 203 25 323.04 32.81 542 179 190 11 346.78 21.59 543 179 193 14 343.05 23.44 544 179 202 23 323.10 32.82 545 179 203 24 323.04 32.81 546 179 205 26 323.04 32.81 547 179 208 29 315.89 31.58 548 180 183 3 883.55 5.25 549 180 189 9 347.22 21.41 550 180 190 10 346.86 21.56 551 180 193 13 342.95 23.45 552 180 202 22 323.11 32.81 553 182 185 3 873.26 0.10 554 182 187 5 827.37 1.39 555 182 189 7 357.16 16.45 556 186 231 45 246.82 15.00 557 186 232 46 243.40 13.33 558 186 235 49 229.54 16.30 559 187 228 41 249.46 16.32 560 187 231 44 246.82 15.00 561 187 232 45 243.44 13.35 562 188 191 3 891.61 4.63 563 188 192 4 877.74 1.76 564 188 224 36 256.55 17.43 565 188 225 37 256.50 17.45 566 188 228 40 252.55 17.88 567 188 231 43 243.50 13.35 568 189 192 3 886.44 7.22 569 189 193 4 870.82 1.71 570 190 193 3 886.98 6.95 571 190 194 4 870.87 1.69 572 190 208 18 314.12 32.03 573 190 211 21 307.48 28.78 574 191 194 3 878.01 2.46 575 191 195 4 870.83 1.71 576 191 221 30 258.12 16.73 577 191 224 33 256.76 17.39 578 191 225 34 256.57 17.47 579 191 231 40 243.36 13.26 580 192 195 3 866.86 3.69 581 192 196 4 866.85 3.69 582 192 225 33 256.58 17.46 583 193 196 3 864.51 4.25 584 193 197 4 858.98 0.24 585 193 199 6 832.25 1.69 586 193 202 9 323.16 32.81 587 193 204 11 323.08 32.83 588 194 197 3 872.33 3.23 589 194 204 10 323.04 32.84 590 194 208 14 315.44 31.80 591 194 211 17 307.50 28.76 592 194 212 18 304.07 27.14 593 195 212 17 304.53 27.38 594 195 218 23 259.43 16.22 595 195 221 26 259.12 16.29 596 195 222 27 259.12 16.29 597 195 225 30 257.36 17.16 598 197 204 7 353.82 17.50 599 198 204 6 353.80 17.51 600 198 209 11 322.41 32.91 601 199 202 3 891.03 5.65 602 204 208 4 855.14 1.15 603 204 209 5 840.98 7.47 604 205 208 3 863.33 2.91 605 205 209 4 863.31 2.93 606 206 209 3 872.27 2.90 607 206 210 4 867.87 0.69 608 207 210 3 892.84 2.77 609 207 211 4 871.65 2.58 610 208 211 3 892.84 2.77 611 209 212 3 874.90 5.04 612 209 213 4 870.83 3.00 613 210 213 3 879.52 2.73 614 210 214 4 855.11 7.09 615 211 217 6 305.83 7.02 616 211 218 7 259.39 16.21 617 211 221 10 259.17 16.32 618 212 218 6 259.39 16.21 619 212 221 9 259.19 16.30 620 214 217 3 856.10 2.71 621 214 218 4 835.33 2.69 622 215 219 4 872.70 0.61 623 216 219 3 904.16 0.31 624 216 220 4 869.12 1.18 625 217 220 3 880.78 2.71 626 217 221 4 863.37 0.53 627 218 221 3 874.68 0.34 628 218 222 4 863.11 0.40 629 219 222 3 868.32 0.77 630 219 223 4 851.60 5.35 631 220 223 3 858.77 1.73 632 220 224 4 847.65 3.38 633 221 224 3 881.71 1.26 634 221 225 4 844.39 5.01 635 222 225 3 881.71 1.26 636 222 226 4 844.39 5.02 637 223 226 3 874.57 4.08 638 223 227 4 843.83 4.75 639 223 230 7 737.39 6.06 640 224 227 3 874.57 4.08 641 224 230 6 731.05 9.23 642 224 231 7 438.14 101.92 643 224 234 10 659.83 3.52 644 227 230 3 883.53 4.00 645 227 231 4 873.38 0.90 646 228 231 3 873.55 0.98 647 228 232 4 854.31 10.60 648 229 232 3 877.27 2.77 649 229 233 4 854.31 10.60 650 230 233 3 873.62 0.95 651 230 234 4 861.46 3.98 652 231 234 3 873.49 0.88 653 231 235 4 853.50 0.02 654 232 235 3 864.75 5.25 655 232 236 4 864.35 5.44 656 233 236 3 864.36 5.44 657 233 237 4 864.36 5.44 658 234 237 3 870.94 2.15 659 234 238 4 864.59 5.32 660 235 238 3 864.65 5.34 661 235 239 4 844.15 4.92 662 236 239 3 864.68 5.33 663 236 240 4 849.32 2.34 664 237 240 3 873.48 0.94 665 237 241 4 864.64 5.31 666 238 241 3 864.69 5.29 667 238 242 4 854.30 0.17 668 239 242 3 865.69 2.93 669 239 243 4 859.97 2.67 670 240 243 3 871.33 2.23 671 240 244 4 857.19 6.71 672 241 244 3 868.24 0.83 673 241 245 4 837.83 2.98 674 242 245 3 878.05 5.73 675 244 247 3 837.00 4.99 676 246 250 4 384.58 136.92 677 247 250 3 870.52 0.86