BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 51 50 30.38 1.90 2 2 20 18 25.30 1.42 3 2 21 19 24.75 1.68 4 2 24 22 24.75 1.68 5 2 48 46 27.70 2.98 6 2 49 47 27.70 2.98 7 2 50 48 27.70 2.98 8 2 51 49 31.57 1.38 9 3 51 48 31.07 1.62 10 3 53 50 45.66 2.42 11 4 9 5 810.76 3.29 12 4 13 9 46.05 1.96 13 4 14 10 39.41 0.15 14 4 17 13 35.97 0.24 15 4 51 47 36.99 0.74 16 4 52 48 36.74 0.62 17 4 53 49 45.74 2.46 18 4 129 125 60.38 1.16 19 4 132 128 60.27 1.12 20 5 9 4 810.76 3.29 21 5 53 48 51.52 0.43 22 6 9 3 808.48 2.25 23 6 53 47 60.37 1.17 24 6 54 48 60.94 0.89 25 6 55 49 60.98 0.88 26 6 129 123 60.94 0.89 27 6 132 126 60.95 0.88 28 6 133 127 60.97 0.89 29 6 136 130 60.98 0.89 30 7 55 48 61.21 0.85 31 9 13 4 807.00 1.42 32 9 129 120 75.20 0.83 33 10 13 3 846.11 1.88 34 10 14 4 832.70 6.34 35 10 176 166 92.79 0.12 36 11 14 3 837.12 2.91 37 11 15 4 828.38 6.15 38 11 172 161 80.81 0.88 39 11 175 164 90.89 0.46 40 11 176 165 98.64 0.34 41 12 15 3 835.11 2.79 42 12 16 4 815.63 0.22 43 13 16 3 827.38 5.79 44 13 17 4 809.80 0.29 45 14 17 3 836.32 1.32 46 14 18 4 822.36 2.90 47 14 129 115 99.49 0.01 48 14 157 143 97.75 0.07 49 14 161 147 95.23 0.03 50 14 168 154 96.58 0.69 51 14 171 157 98.67 0.36 52 14 172 158 99.38 0.01 53 15 18 3 837.44 0.77 54 15 19 4 822.37 2.90 55 15 168 153 97.78 0.09 56 15 172 157 99.60 0.09 57 16 19 3 843.34 2.19 58 16 20 4 834.29 0.61 59 17 20 3 837.25 0.85 60 17 21 4 833.34 1.09 61 17 50 33 104.09 0.70 62 17 52 35 114.81 0.30 63 17 158 141 100.06 0.12 64 18 21 3 834.29 0.62 65 18 22 4 833.36 1.08 66 18 157 139 101.69 0.36 67 18 158 140 100.06 0.12 68 18 161 143 100.03 0.12 69 18 162 144 99.45 0.03 70 18 168 150 100.05 0.12 71 19 22 3 845.44 6.20 72 19 23 4 833.37 1.08 73 20 23 3 841.76 0.92 74 20 24 4 824.80 3.21 75 21 24 3 841.93 0.49 76 21 25 4 835.58 2.18 77 21 48 27 119.52 0.54 78 21 50 29 119.55 0.56 79 21 158 137 117.78 1.13 80 21 162 141 111.04 0.17 81 22 25 3 850.22 1.78 82 22 161 139 118.51 0.93 83 22 162 140 113.81 0.65 84 22 165 143 118.60 0.88 85 22 168 146 118.81 0.92 86 22 238 216 106.34 0.40 87 22 239 217 103.81 0.87 88 24 48 24 121.79 0.12 89 25 37 12 121.27 0.01 90 25 48 23 121.81 0.12 91 25 66 41 121.08 0.12 92 25 69 44 120.92 0.17 93 25 239 214 120.25 0.39 94 26 37 11 121.83 0.14 95 26 38 12 121.91 0.15 96 26 41 15 122.00 0.10 97 26 66 40 121.86 0.15 98 27 30 3 945.89 2.08 99 27 35 8 121.88 0.15 100 27 36 9 121.86 0.15 101 27 38 11 121.98 0.12 102 28 38 10 122.02 0.10 103 29 35 6 336.99 107.14 104 29 36 7 336.80 107.19 105 30 33 3 828.36 4.15 106 30 34 4 554.71 100.71 107 30 35 5 336.98 107.14 108 30 36 6 336.97 107.13 109 31 34 3 837.85 1.27 110 31 36 5 338.56 107.89 111 31 199 168 149.14 2.25 112 31 200 169 138.75 0.43 113 31 203 172 159.16 0.95 114 31 240 209 124.19 0.52 115 32 199 167 158.97 0.96 116 32 202 170 159.41 0.89 117 32 203 171 159.41 0.89 118 32 207 175 159.44 0.90 119 32 228 196 159.44 0.90 120 34 199 165 159.49 0.89 121 34 233 199 159.61 0.86 122 34 237 203 159.40 0.91 123 34 238 204 159.27 0.96 124 34 239 205 159.26 0.95 125 34 240 206 159.06 1.00 126 35 238 203 159.50 0.88 127 35 239 204 159.31 0.94 128 35 240 205 159.31 0.94 129 36 200 164 159.61 0.84 130 36 240 204 159.32 0.94 131 36 242 206 158.97 1.03 132 37 66 29 159.48 0.85 133 37 67 30 159.44 0.88 134 37 68 31 159.44 0.88 135 37 69 32 159.47 0.89 136 37 192 155 159.63 0.86 137 37 239 202 159.62 0.85 138 37 240 203 159.55 0.85 139 37 242 205 159.19 0.95 140 38 41 3 902.80 2.21 141 38 67 29 159.58 0.83 142 38 242 204 159.30 0.96 143 39 67 28 351.12 94.89 144 39 242 203 159.31 0.96 145 39 244 205 159.21 0.96 146 39 245 206 158.55 1.06 147 40 46 6 742.57 14.67 148 40 67 27 351.22 94.92 149 41 45 4 828.75 5.93 150 41 46 5 666.93 52.49 151 41 66 25 542.31 0.60 152 41 67 26 542.32 0.60 153 43 46 3 866.12 0.77 154 43 47 4 866.12 0.77 155 43 94 51 491.71 0.23 156 44 47 3 866.11 0.77 157 44 94 50 495.13 1.49 158 45 66 21 560.55 5.71 159 46 65 19 560.58 5.71 160 46 66 20 560.53 5.73 161 46 67 21 560.51 5.73 162 47 64 17 560.61 5.71 163 47 65 18 560.56 5.72 164 47 66 19 560.56 5.72 165 47 72 25 559.98 5.67 166 47 90 43 537.34 0.54 167 47 91 44 537.28 0.55 168 47 94 47 536.83 0.34 169 47 95 48 536.28 0.27 170 48 62 14 561.31 5.55 171 48 63 15 560.94 5.58 172 48 64 16 560.62 5.71 173 48 66 18 560.57 5.72 174 49 61 12 561.33 5.54 175 49 62 13 561.30 5.55 176 49 63 14 561.20 5.53 177 49 95 46 537.38 0.55 178 49 96 47 537.36 0.55 179 50 61 11 561.47 5.48 180 50 62 12 561.31 5.55 181 50 64 14 561.25 5.53 182 50 71 21 560.50 5.70 183 50 154 104 501.42 0.73 184 50 158 108 462.27 0.58 185 51 60 9 561.60 5.46 186 51 61 10 561.48 5.48 187 52 59 7 561.75 5.50 188 52 60 8 561.69 5.50 189 52 62 10 561.59 5.46 190 52 136 84 513.56 0.70 191 52 154 102 513.32 0.62 192 52 158 106 474.92 2.41 193 53 57 4 590.52 17.91 194 53 58 5 563.92 5.21 195 53 59 6 563.15 4.84 196 53 136 83 514.04 0.47 197 54 57 3 805.52 0.69 198 54 58 4 575.49 0.24 199 54 133 79 513.48 0.68 200 54 136 82 514.03 0.47 201 54 137 83 514.19 0.39 202 55 130 75 513.99 0.45 203 55 133 78 514.00 0.45 204 58 140 82 514.99 0.65 205 59 140 81 514.84 0.70 206 60 75 15 559.78 5.59 207 60 76 16 559.74 5.59 208 60 136 76 513.58 0.70 209 60 139 79 514.64 0.62 210 60 140 80 514.66 0.62 211 61 74 13 559.88 5.62 212 61 75 14 559.77 5.59 213 61 96 35 537.43 0.57 214 62 71 9 560.44 5.74 215 62 73 11 560.00 5.67 216 62 74 12 559.82 5.63 217 62 154 92 502.55 1.26 218 63 71 8 560.37 5.71 219 63 72 9 560.07 5.66 220 63 73 10 559.94 5.67 221 63 95 32 537.38 0.55 222 63 96 33 537.38 0.55 223 64 69 5 560.45 5.72 224 64 70 6 560.39 5.72 225 64 71 7 560.11 5.67 226 65 68 3 852.31 1.03 227 65 69 4 609.03 14.50 228 65 70 5 560.33 5.73 229 65 72 7 559.98 5.67 230 67 241 174 171.72 0.51 231 67 245 178 160.24 0.41 232 68 163 95 443.78 1.20 233 68 192 124 309.56 3.04 234 68 241 173 173.11 1.20 235 69 159 90 451.23 2.36 236 69 162 93 448.72 1.25 237 69 163 94 443.77 1.20 238 69 192 123 311.73 2.08 239 69 239 170 192.53 1.30 240 70 85 15 538.06 0.71 241 70 109 39 509.24 1.32 242 70 110 40 509.17 1.28 243 71 83 12 538.37 0.71 244 71 154 83 501.40 0.81 245 71 155 84 491.39 0.36 246 71 158 87 457.99 2.26 247 71 159 88 454.55 0.71 248 72 82 10 538.37 0.71 249 72 83 11 538.33 0.70 250 72 87 15 537.61 0.51 251 72 88 16 537.52 0.54 252 72 91 19 537.52 0.54 253 73 81 8 538.43 0.70 254 73 82 9 538.33 0.73 255 73 96 23 537.45 0.56 256 74 80 6 555.26 9.06 257 74 81 7 538.49 0.69 258 74 96 22 537.44 0.57 259 74 105 31 517.07 0.86 260 74 139 65 514.65 0.62 261 74 151 77 514.13 0.49 262 75 79 4 763.48 3.75 263 75 80 5 555.30 9.04 264 75 96 21 537.44 0.57 265 75 99 24 537.17 0.52 266 76 79 3 851.38 0.24 267 76 81 5 716.40 36.52 268 76 139 63 514.63 0.61 269 76 140 64 514.62 0.62 270 78 99 21 537.21 0.51 271 78 100 22 537.20 0.51 272 78 101 23 537.15 0.51 273 79 99 20 537.21 0.51 274 79 101 22 536.04 0.42 275 79 103 24 520.75 2.28 276 80 96 16 537.46 0.57 277 80 99 19 537.19 0.50 278 80 101 21 536.04 0.42 279 80 102 22 517.52 0.86 280 80 103 23 517.31 0.96 281 81 103 22 517.23 0.93 282 81 105 24 517.05 0.87 283 81 142 61 516.49 1.02 284 82 88 6 549.31 5.27 285 82 91 9 549.30 5.27 286 82 92 10 549.29 5.28 287 82 95 13 537.62 0.56 288 82 96 14 537.53 0.52 289 82 102 20 517.71 1.16 290 82 103 21 517.15 0.89 291 82 104 22 517.05 0.87 292 82 105 23 516.94 0.85 293 83 88 5 718.08 3.35 294 83 105 22 516.94 0.85 295 83 107 24 516.42 1.01 296 83 108 25 516.11 1.14 297 83 109 26 516.11 1.13 298 83 114 31 516.19 1.10 299 83 151 68 514.84 0.69 300 84 104 20 517.01 0.88 301 84 105 21 516.96 0.86 302 84 107 23 516.76 0.82 303 84 108 24 516.21 1.09 304 85 88 3 851.30 0.05 305 85 89 4 832.06 2.21 306 85 108 23 516.18 1.10 307 85 109 24 516.18 1.10 308 86 89 3 845.26 3.06 309 86 90 4 833.38 2.87 310 87 90 3 834.81 2.18 311 87 91 4 835.70 1.74 312 88 91 3 835.70 1.74 313 88 92 4 835.70 1.74 314 89 92 3 841.66 2.06 315 89 93 4 841.10 2.35 316 89 102 13 517.52 0.86 317 90 93 3 849.49 1.57 318 90 94 4 847.34 1.96 319 91 94 3 857.97 3.35 320 91 95 4 835.61 1.74 321 92 97 5 809.87 0.58 322 92 102 10 517.28 0.95 323 93 97 4 833.70 1.75 324 96 99 3 862.00 2.33 325 103 142 39 688.14 1.52 326 105 142 37 704.80 1.16 327 105 151 46 697.87 0.73 328 106 142 36 705.14 1.04 329 106 144 38 705.10 1.06 330 107 113 6 748.63 12.10 331 107 114 7 742.84 15.00 332 107 117 10 710.12 0.90 333 107 144 37 705.20 1.05 334 107 147 40 705.22 1.07 335 107 148 41 705.15 1.07 336 107 151 44 705.15 1.07 337 108 113 5 809.69 0.95 338 108 114 6 808.32 1.65 339 109 114 5 809.72 0.97 340 110 113 3 859.61 1.10 341 110 114 4 826.93 3.16 342 111 114 3 843.81 0.35 343 111 115 4 834.06 5.21 344 111 122 11 705.32 1.08 345 111 152 41 705.30 1.10 346 111 155 44 705.29 1.10 347 111 156 45 705.27 1.11 348 111 160 49 654.37 7.70 349 111 189 78 328.69 5.44 350 112 115 3 844.24 0.56 351 112 116 4 840.42 2.03 352 113 116 3 840.87 2.25 353 113 117 4 838.99 3.19 354 114 117 3 841.27 2.04 355 114 118 4 811.47 0.23 356 114 148 34 705.47 1.17 357 114 151 37 705.46 1.17 358 114 152 38 705.44 1.16 359 114 155 41 705.45 1.17 360 115 118 3 841.35 2.00 361 115 119 4 810.89 0.43 362 115 120 5 761.36 3.93 363 115 121 6 707.62 2.22 364 115 122 7 707.63 2.23 365 115 152 37 705.48 1.19 366 116 119 3 840.52 1.59 367 117 145 28 705.98 1.31 368 117 148 31 705.97 1.31 369 118 148 30 705.97 1.31 370 118 149 31 705.96 1.31 371 118 152 34 705.96 1.31 372 120 124 4 807.46 2.83 373 120 125 5 807.03 2.61 374 121 124 3 862.97 0.48 375 121 125 4 842.74 2.14 376 122 125 3 853.43 0.00 377 122 152 30 706.07 1.29 378 122 156 34 705.97 1.26 379 122 181 59 415.67 0.73 380 122 186 64 356.56 6.24 381 122 189 67 351.29 3.82 382 122 190 68 351.25 3.80 383 123 180 57 416.77 0.95 384 123 181 58 415.83 0.81 385 123 186 63 370.41 8.78 386 124 180 56 417.53 1.14 387 125 152 27 707.50 1.50 388 125 153 28 707.47 1.50 389 125 180 55 416.47 1.11 390 125 181 56 416.47 1.12 391 126 131 5 813.13 3.41 392 126 153 27 707.52 1.50 393 126 178 52 419.01 0.95 394 126 179 53 418.95 0.95 395 126 180 54 415.86 0.82 396 127 131 4 813.36 3.50 397 127 153 26 707.52 1.50 398 127 156 29 707.51 1.50 399 127 157 30 707.46 1.51 400 127 171 44 430.34 0.49 401 127 174 47 427.62 1.49 402 127 178 51 419.00 0.95 403 127 179 52 418.75 0.87 404 127 181 54 415.37 0.89 405 128 131 3 843.43 3.18 406 128 132 4 814.37 3.00 407 128 157 29 707.47 1.51 408 128 171 43 430.19 0.42 409 128 174 46 426.72 1.05 410 128 178 50 419.00 0.95 411 129 132 3 829.75 3.23 412 129 133 4 814.37 3.00 413 129 171 42 430.19 0.42 414 129 174 45 427.59 1.48 415 129 175 46 426.19 0.83 416 130 133 3 835.58 0.32 417 130 134 4 815.30 3.46 418 130 174 44 427.62 1.49 419 130 175 45 426.59 1.00 420 130 176 46 426.19 0.83 421 130 177 47 424.81 0.25 422 130 178 48 419.11 0.91 423 131 134 3 831.06 1.94 424 131 135 4 822.56 0.17 425 131 153 22 707.73 1.46 426 131 178 47 419.00 0.95 427 132 135 3 824.83 0.96 428 132 136 4 827.78 2.27 429 132 153 21 707.75 1.47 430 132 154 22 707.73 1.46 431 132 157 25 707.58 1.46 432 133 136 3 844.65 4.24 433 133 137 4 827.81 2.27 434 134 137 3 835.16 1.40 435 134 138 4 827.81 2.27 436 135 138 3 834.47 1.06 437 135 139 4 827.80 2.27 438 135 150 15 707.80 1.49 439 135 153 18 707.80 1.49 440 136 139 3 834.22 0.94 441 136 140 4 827.77 2.29 442 137 140 3 844.60 0.31 443 138 141 3 865.05 9.92 444 138 146 8 708.25 1.61 445 138 147 9 708.23 1.60 446 138 150 12 708.19 1.61 447 139 147 8 708.23 1.60 448 139 150 11 708.22 1.59 449 139 151 12 708.20 1.60 450 139 154 15 708.20 1.60 451 143 146 3 853.73 2.88 452 143 147 4 824.65 1.11 453 144 147 3 829.72 1.38 454 144 148 4 842.37 4.95 455 145 148 3 845.84 3.67 456 145 149 4 845.23 3.98 457 146 149 3 845.84 3.67 458 146 150 4 845.22 3.98 459 147 150 3 845.23 3.98 460 147 151 4 844.75 3.75 461 148 151 3 852.88 0.32 462 148 152 4 844.70 3.73 463 149 152 3 854.98 1.43 464 149 153 4 844.65 3.72 465 150 153 3 843.62 0.70 466 150 154 4 838.10 0.46 467 151 154 3 842.48 1.73 468 151 155 4 832.30 2.44 469 152 155 3 851.01 2.64 470 152 156 4 836.12 4.35 471 153 156 3 865.46 4.12 472 153 157 4 835.97 4.42 473 154 157 3 863.19 5.26 474 154 158 4 833.37 5.73 475 155 158 3 861.09 1.95 476 155 159 4 808.35 5.58 477 155 160 5 804.32 7.59 478 156 160 4 786.59 2.34 479 156 161 5 804.62 4.12 480 156 167 11 430.70 0.31 481 156 181 25 402.88 0.60 482 156 186 30 342.20 0.20 483 156 189 33 328.58 5.33 484 157 161 4 822.89 1.91 485 157 171 14 430.12 0.42 486 158 161 3 848.05 5.25 487 158 162 4 841.54 7.42 488 159 162 3 852.53 1.92 489 159 163 4 841.54 7.42 490 160 163 3 843.61 6.38 491 160 164 4 837.82 9.27 492 160 167 7 753.47 12.67 493 160 189 29 327.77 4.94 494 161 164 3 854.37 1.00 495 161 167 6 498.45 33.56 496 161 168 7 430.31 0.48 497 162 238 76 230.03 2.12 498 162 239 77 229.66 2.16 499 163 192 29 313.08 1.41 500 163 237 74 231.41 2.35 501 163 238 75 229.76 2.11 502 163 239 76 228.71 1.68 503 164 167 3 846.71 6.55 504 164 168 4 827.33 0.41 505 164 188 24 341.54 0.32 506 164 189 25 339.44 0.03 507 164 192 28 320.23 1.79 508 164 195 31 322.29 2.90 509 164 236 72 231.94 2.14 510 164 237 73 231.67 2.21 511 164 238 74 230.58 2.40 512 165 168 3 828.44 0.96 513 165 169 4 828.09 0.04 514 165 235 70 232.02 2.15 515 165 236 71 231.94 2.14 516 165 238 73 231.34 2.36 517 166 169 3 830.95 0.29 518 166 170 4 828.28 0.10 519 166 182 16 408.91 0.40 520 166 184 18 384.37 1.86 521 166 185 19 370.19 8.93 522 166 188 22 342.24 0.17 523 166 218 52 271.05 6.30 524 166 236 70 231.99 2.17 525 167 170 3 828.29 0.10 526 167 171 4 835.50 3.71 527 167 185 18 364.28 8.46 528 167 188 21 342.22 0.18 529 167 189 22 341.79 0.40 530 168 171 3 853.00 3.29 531 168 172 4 828.28 0.11 532 169 172 3 835.49 3.72 533 169 173 4 828.26 0.12 534 170 173 3 851.85 4.46 535 170 174 4 827.55 0.46 536 170 182 12 413.23 0.97 537 170 185 15 367.70 10.17 538 171 174 3 827.50 0.43 539 171 175 4 827.50 0.43 540 172 175 3 838.75 9.69 541 174 177 3 840.34 8.89 542 174 178 4 658.86 40.98 543 174 179 5 659.76 40.53 544 174 180 6 659.76 40.53 545 174 181 7 578.98 80.92 546 181 185 4 806.51 6.24 547 181 186 5 806.50 6.21 548 182 185 3 841.76 5.63 549 182 186 4 806.57 6.21 550 183 186 3 835.47 4.73 551 183 187 4 806.53 6.20 552 183 216 33 271.76 6.63 553 183 221 38 271.83 6.60 554 184 187 3 835.46 4.74 555 184 188 4 815.72 5.21 556 184 216 32 271.74 6.65 557 184 217 33 271.75 6.66 558 184 218 34 271.86 6.71 559 185 188 3 829.87 1.86 560 185 189 4 826.82 0.30 561 186 189 3 838.93 2.60 562 186 190 4 826.83 0.29 563 187 190 3 839.27 2.43 564 187 191 4 835.08 0.90 565 187 215 28 271.73 6.66 566 187 216 29 271.77 6.68 567 188 191 3 835.09 0.90 568 188 192 4 835.08 0.90 569 188 215 27 272.56 7.08 570 188 232 44 232.55 1.91 571 188 236 48 232.10 2.12 572 191 194 3 834.27 7.10 573 191 195 4 820.47 1.07 574 191 213 22 277.75 7.45 575 191 215 24 277.76 7.46 576 192 195 3 842.59 4.11 577 192 196 4 821.22 1.45 578 192 237 45 232.24 2.05 579 192 239 47 232.03 2.14 580 192 241 49 221.97 0.67 581 193 196 3 842.24 8.29 582 193 197 4 825.68 0.01 583 193 241 48 220.69 1.20 584 193 246 53 173.98 1.33 585 194 197 3 842.24 8.29 586 194 198 4 825.69 0.01 587 194 213 19 284.30 10.14 588 195 198 3 836.18 3.30 589 195 199 4 824.59 0.57 590 195 209 14 286.56 10.36 591 195 213 18 284.58 10.29 592 195 215 20 284.72 10.35 593 195 225 30 284.25 10.18 594 195 232 37 233.06 1.80 595 195 237 42 232.31 2.02 596 196 199 3 856.63 0.07 597 196 200 4 828.29 2.19 598 196 237 41 232.42 2.01 599 196 240 44 229.62 2.12 600 196 241 45 228.91 1.81 601 196 246 50 183.81 1.54 602 197 200 3 849.58 3.46 603 197 201 4 828.30 2.19 604 197 243 46 220.51 1.13 605 197 246 49 188.65 0.27 606 198 201 3 829.39 1.64 607 198 202 4 828.30 2.18 608 198 209 11 286.58 10.36 609 198 210 12 286.58 10.36 610 198 211 13 286.58 10.36 611 198 213 15 285.11 10.25 612 199 202 3 847.89 4.67 613 199 203 4 841.28 1.37 614 199 209 10 286.61 10.35 615 199 228 29 283.36 9.98 616 199 233 34 237.66 1.60 617 199 237 38 233.22 2.09 618 199 240 41 232.34 2.01 619 200 203 3 864.62 3.94 620 200 240 40 232.38 2.01 621 200 241 41 232.37 2.01 622 200 242 42 232.12 2.03 623 200 243 43 231.51 1.73 624 201 205 4 828.38 5.08 625 201 210 9 288.64 9.35 626 201 243 42 231.99 1.97 627 202 205 3 813.02 2.21 628 202 206 4 808.30 0.15 629 202 207 5 309.04 0.83 630 202 208 6 292.33 7.52 631 202 209 7 288.64 9.36 632 202 228 26 284.49 10.45 633 204 210 6 763.60 3.78 634 205 208 3 833.33 3.59 635 205 210 5 763.60 3.78 636 207 227 20 284.60 10.48 637 207 228 21 284.57 10.47 638 208 226 18 284.67 10.46 639 208 227 19 284.57 10.46 640 209 212 3 854.34 2.46 641 209 213 4 862.60 1.67 642 209 225 16 284.89 10.38 643 209 226 17 284.64 10.46 644 209 227 18 284.57 10.46 645 209 228 19 284.57 10.46 646 209 233 24 239.93 2.00 647 212 224 12 285.06 10.36 648 212 225 13 285.00 10.34 649 212 226 14 284.75 10.42 650 213 224 11 285.10 10.35 651 213 225 12 285.00 10.34 652 214 222 8 285.49 10.49 653 214 223 9 285.26 10.39 654 214 224 10 285.10 10.35 655 215 222 7 285.51 10.48 656 215 223 8 285.47 10.50 657 215 225 10 285.25 10.39 658 215 232 17 234.96 0.98 659 216 220 4 707.75 31.10 660 216 221 5 285.54 10.49 661 216 222 6 285.53 10.49 662 216 223 7 285.51 10.48 663 217 220 3 842.20 8.51 664 217 221 4 622.07 82.96 665 217 223 6 285.55 10.50 666 225 230 5 324.83 16.02 667 225 232 7 301.62 4.42 668 225 233 8 324.83 16.02 669 227 230 3 865.56 0.17 670 227 233 6 463.86 53.47 671 228 233 5 463.86 53.47 672 228 234 6 549.38 96.23 673 229 234 5 549.48 96.28 674 230 233 3 830.12 6.04 675 230 234 4 812.06 0.51 676 231 234 3 826.47 7.71 677 231 235 4 766.62 0.65 678 231 236 5 753.68 5.83 679 232 236 4 753.68 5.83 680 232 237 5 753.64 5.82 681 233 237 4 758.08 3.62 682 235 238 3 981.50 3.08 683 237 240 3 935.02 3.23 684 241 245 4 831.58 2.74 685 241 246 5 798.13 3.97 686 242 245 3 851.36 3.93 687 242 246 4 839.23 6.14 688 243 246 3 839.25 6.13