BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 226 223 113.70 0.61 2 3 228 225 113.58 0.56 3 3 229 226 112.50 0.02 4 4 204 200 170.22 0.36 5 4 205 201 167.64 1.60 6 4 206 202 165.88 2.48 7 4 207 203 161.91 0.98 8 4 229 225 114.25 0.42 9 5 36 31 465.55 0.94 10 5 37 32 465.55 0.93 11 5 38 33 465.55 0.93 12 5 207 202 168.59 1.12 13 6 36 30 465.60 0.94 14 6 37 31 465.56 0.93 15 6 38 32 465.56 0.93 16 6 223 217 114.37 0.45 17 6 229 223 114.36 0.45 18 6 249 243 24.90 0.52 19 7 37 30 465.61 0.93 20 7 38 31 465.56 0.93 21 7 40 33 465.61 0.93 22 7 90 83 271.07 41.38 23 7 163 156 261.35 39.05 24 7 207 200 182.91 0.03 25 7 209 202 169.83 0.36 26 7 229 222 114.66 0.49 27 7 230 223 114.33 0.43 28 7 231 224 114.33 0.43 29 8 37 29 465.66 0.92 30 8 38 30 465.64 0.92 31 8 39 31 465.58 0.94 32 8 40 32 465.62 0.93 33 8 231 223 114.37 0.44 34 8 242 234 58.63 0.10 35 8 246 238 41.64 1.35 36 9 40 31 465.62 0.93 37 9 124 115 269.76 41.82 38 9 231 222 114.98 0.36 39 9 232 223 114.33 0.42 40 9 233 224 114.31 0.42 41 9 242 233 58.83 0.01 42 10 40 30 465.67 0.92 43 10 41 31 465.62 0.93 44 10 42 32 465.64 0.92 45 10 237 227 87.49 0.79 46 10 242 232 58.83 0.01 47 11 42 31 465.71 0.89 48 11 65 54 464.78 0.84 49 11 95 84 270.38 41.61 50 11 232 221 117.18 0.06 51 11 233 222 114.89 0.41 52 11 234 223 114.31 0.42 53 11 237 226 93.52 0.40 54 12 15 3 888.39 2.80 55 12 21 9 467.47 1.11 56 12 24 12 467.24 0.99 57 12 25 13 467.47 1.10 58 12 28 16 467.24 0.99 59 12 41 29 465.85 0.94 60 12 42 30 465.85 0.94 61 12 43 31 465.72 0.88 62 12 65 53 464.91 0.80 63 12 237 225 110.12 1.95 64 12 238 226 91.18 0.77 65 13 44 31 465.84 0.94 66 13 65 52 464.71 0.90 67 13 97 84 270.49 41.68 68 13 234 221 117.02 0.13 69 14 44 30 465.86 0.94 70 15 44 29 465.88 0.95 71 15 238 223 114.22 0.38 72 16 21 5 822.27 0.79 73 16 47 31 466.00 0.96 74 17 20 3 855.37 1.09 75 17 21 4 845.26 3.96 76 18 21 3 868.93 2.65 77 18 22 4 845.25 3.96 78 18 50 32 466.82 1.06 79 19 22 3 871.07 1.57 80 19 23 4 860.27 3.55 81 20 23 3 869.14 0.89 82 20 24 4 860.29 3.54 83 21 24 3 865.45 0.96 84 21 25 4 848.89 2.16 85 21 47 26 467.24 1.54 86 21 50 29 468.82 0.94 87 21 238 217 112.41 0.04 88 22 25 3 860.30 3.54 89 22 26 4 855.04 6.16 90 22 50 28 469.87 0.41 91 22 54 32 469.92 0.41 92 23 26 3 855.62 1.20 93 23 27 4 849.77 3.85 94 24 27 3 869.07 2.80 95 24 28 4 846.63 2.28 96 24 237 213 112.42 0.06 97 24 238 214 107.77 1.76 98 24 239 215 90.72 0.07 99 24 240 216 84.84 0.72 100 25 28 3 855.47 1.01 101 25 29 4 848.81 3.38 102 25 51 26 470.19 0.42 103 25 54 29 470.29 0.42 104 25 55 30 470.28 0.42 105 26 29 3 851.55 2.01 106 26 30 4 851.53 2.01 107 26 54 28 470.32 0.43 108 27 30 3 857.22 0.70 109 27 31 4 851.53 2.01 110 27 240 213 80.86 0.56 111 27 243 216 66.18 1.20 112 28 31 3 859.89 3.33 113 28 41 13 470.55 0.55 114 28 55 27 471.09 0.66 115 28 57 29 471.11 0.66 116 28 60 32 471.05 0.64 117 28 237 209 107.97 1.69 118 28 242 214 66.09 1.22 119 28 243 215 58.87 0.03 120 29 54 25 471.09 0.66 121 29 55 26 471.09 0.66 122 29 56 27 471.09 0.66 123 29 57 28 471.08 0.66 124 31 57 26 473.43 1.76 125 31 243 212 57.02 0.92 126 31 247 216 54.23 2.30 127 33 37 4 889.80 3.17 128 33 39 6 704.97 64.70 129 33 57 24 537.19 0.07 130 33 59 26 498.40 10.36 131 33 60 27 512.38 3.37 132 33 246 213 49.27 0.07 133 35 59 24 572.48 1.40 134 35 246 211 49.43 0.01 135 35 247 212 44.23 1.53 136 35 249 214 40.02 0.60 137 35 250 215 29.15 1.23 138 37 59 22 572.61 1.34 139 37 246 209 42.33 1.68 140 37 249 212 30.70 2.30 141 38 59 21 572.65 1.32 142 38 60 22 572.58 1.36 143 38 61 23 572.57 1.36 144 38 90 52 271.16 41.36 145 39 59 20 572.68 1.33 146 39 60 21 572.62 1.34 147 39 61 22 572.58 1.36 148 39 246 207 41.63 1.33 149 40 61 21 572.63 1.33 150 40 63 23 572.49 1.39 151 40 90 50 271.35 41.26 152 40 124 84 269.43 41.86 153 41 51 10 572.88 1.36 154 41 55 14 572.84 1.32 155 41 60 19 572.76 1.32 156 41 61 20 572.72 1.32 157 41 62 21 572.58 1.36 158 41 63 22 572.57 1.36 159 42 63 21 572.55 1.38 160 42 65 23 572.29 1.38 161 42 92 50 271.43 41.19 162 43 47 4 832.26 1.70 163 43 48 5 698.17 60.79 164 43 63 20 572.70 1.33 165 43 65 22 572.56 1.36 166 44 47 3 866.05 1.18 167 44 48 4 844.92 2.68 168 44 65 21 572.66 1.33 169 45 48 3 859.76 4.74 170 45 64 19 572.75 1.31 171 45 66 21 572.71 1.32 172 45 78 33 293.50 41.03 173 45 83 38 291.47 40.11 174 47 50 3 867.66 0.37 175 47 51 4 846.06 0.84 176 48 51 3 864.58 0.17 177 48 52 4 846.60 0.58 178 48 62 14 572.85 1.30 179 48 63 15 572.85 1.30 180 48 64 16 572.80 1.31 181 48 83 35 290.79 40.52 182 48 86 38 280.56 40.74 183 48 88 40 273.66 41.05 184 49 52 3 870.71 4.65 185 49 53 4 856.15 5.36 186 49 86 37 280.56 40.74 187 50 53 3 877.12 5.11 188 50 54 4 856.16 5.37 189 51 54 3 867.58 0.35 190 51 55 4 838.43 3.37 191 51 62 11 572.86 1.29 192 52 62 10 572.86 1.30 193 52 86 34 280.50 40.73 194 55 60 5 573.00 1.29 195 55 62 7 572.92 1.27 196 57 60 3 869.51 4.67 197 60 242 182 64.20 0.45 198 60 246 186 47.46 0.89 199 61 90 29 271.88 41.72 200 62 88 26 272.41 41.52 201 62 90 28 272.12 41.54 202 63 88 25 273.62 41.01 203 63 90 27 272.06 41.61 204 63 91 28 272.23 41.57 205 64 83 19 281.67 41.27 206 64 88 24 273.94 40.92 207 64 90 26 272.41 41.52 208 64 91 27 272.38 41.50 209 64 92 28 273.73 40.82 210 65 92 27 273.73 40.81 211 66 83 17 293.61 41.09 212 66 91 25 273.88 40.80 213 66 92 26 273.86 40.80 214 66 93 27 273.85 40.79 215 66 98 32 274.07 40.69 216 66 112 46 274.63 40.42 217 67 78 11 293.86 41.19 218 67 79 12 293.82 41.20 219 67 80 13 293.81 41.19 220 67 83 16 293.74 41.16 221 67 91 24 274.42 40.62 222 67 93 26 274.30 40.59 223 67 112 45 274.65 40.41 224 68 74 6 297.80 39.55 225 68 77 9 294.04 41.23 226 68 78 10 293.98 41.20 227 68 79 11 293.92 41.20 228 68 80 12 293.93 41.20 229 68 112 44 274.69 40.40 230 69 79 10 294.00 41.20 231 69 80 11 293.95 41.19 232 69 81 12 293.97 41.20 233 69 115 46 279.91 40.81 234 70 74 4 922.37 2.76 235 70 79 9 294.03 41.19 236 71 74 3 969.14 0.07 237 71 79 8 294.08 41.21 238 71 81 10 294.07 41.21 239 71 82 11 294.08 41.20 240 74 77 3 838.83 2.77 241 78 82 4 845.44 0.70 242 78 83 5 835.72 4.15 243 79 82 3 860.39 1.54 244 79 83 4 845.44 0.71 245 80 83 3 859.78 1.22 246 80 84 4 838.94 2.54 247 80 115 35 356.30 58.89 248 80 116 36 308.39 35.11 249 80 121 41 477.36 0.90 250 81 84 3 858.73 1.32 251 81 85 4 841.48 1.27 252 82 85 3 870.20 3.37 253 82 86 4 845.75 3.39 254 83 86 3 848.59 1.98 255 83 87 4 845.75 3.39 256 83 88 5 627.37 22.09 257 83 91 8 478.89 2.35 258 83 121 38 543.73 19.71 259 84 87 3 843.37 0.84 260 84 119 35 552.87 15.12 261 84 121 37 579.80 1.67 262 88 121 33 660.88 1.28 263 89 120 31 660.98 1.27 264 89 121 32 660.79 1.29 265 89 122 33 660.74 1.31 266 90 121 31 660.93 1.27 267 90 122 32 660.75 1.30 268 90 124 34 660.70 1.32 269 90 163 73 660.73 1.31 270 91 116 25 661.03 1.27 271 91 121 30 661.00 1.28 272 91 122 31 660.96 1.27 273 91 123 32 660.78 1.31 274 91 124 33 660.74 1.31 275 92 95 3 918.72 0.13 276 92 124 32 660.77 1.29 277 92 126 34 660.69 1.32 278 92 232 140 119.80 0.52 279 93 112 19 661.06 1.26 280 93 113 20 661.05 1.27 281 93 116 23 661.13 1.29 282 93 123 30 661.02 1.27 283 93 124 31 660.95 1.27 284 93 125 32 660.74 1.31 285 93 126 33 660.73 1.31 286 94 99 5 827.47 1.16 287 94 104 10 661.06 1.27 288 94 109 15 661.04 1.27 289 94 126 32 660.75 1.30 290 95 98 3 850.43 1.32 291 95 99 4 841.57 0.02 292 95 126 31 660.91 1.28 293 95 167 72 660.72 1.30 294 95 232 137 120.05 0.55 295 96 99 3 850.44 1.33 296 96 100 4 829.56 0.18 297 96 167 71 660.87 1.27 298 96 169 73 655.58 0.40 299 97 100 3 850.36 1.37 300 97 101 4 849.02 0.70 301 97 102 5 827.51 1.19 302 98 102 4 827.49 1.18 303 98 103 5 827.49 1.18 304 98 104 6 661.21 1.28 305 99 104 5 661.11 1.29 306 99 106 7 661.12 1.29 307 99 109 10 661.31 1.32 308 99 126 27 661.14 1.30 309 99 127 28 661.12 1.30 310 99 146 47 661.14 1.30 311 103 108 5 847.72 4.78 312 104 108 4 847.72 4.78 313 104 109 5 835.59 1.27 314 104 112 8 823.33 0.20 315 105 108 3 876.13 0.23 316 105 109 4 860.85 1.79 317 106 109 3 867.66 4.01 318 106 110 4 860.84 1.79 319 106 145 39 661.39 1.32 320 106 146 40 661.36 1.31 321 106 149 43 661.41 1.31 322 107 110 3 860.85 1.79 323 107 111 4 860.84 1.79 324 108 111 3 858.39 3.02 325 108 112 4 854.73 4.83 326 109 112 3 856.02 1.72 327 109 113 4 843.94 1.76 328 109 150 41 661.51 1.33 329 110 113 3 866.30 5.07 330 110 114 4 854.40 2.54 331 110 149 39 661.53 1.32 332 110 150 40 661.51 1.32 333 111 114 3 867.95 4.25 334 111 115 4 860.17 0.35 335 111 153 42 661.91 1.39 336 112 115 3 870.86 2.79 337 112 116 4 852.07 4.41 338 113 116 3 865.78 0.26 339 113 117 4 852.06 4.41 340 113 123 10 661.69 1.32 341 113 125 12 661.58 1.33 342 113 150 37 661.62 1.35 343 113 154 41 663.20 0.85 344 114 117 3 864.96 1.34 345 114 118 4 854.03 2.31 346 114 153 39 663.13 0.87 347 114 154 40 663.15 0.87 348 115 118 3 876.00 4.19 349 115 119 4 863.14 2.25 350 116 119 3 876.00 4.18 351 116 120 4 854.29 6.67 352 116 121 5 754.00 30.55 353 116 123 7 677.13 7.86 354 117 120 3 854.48 6.58 355 117 123 6 677.36 7.75 356 117 154 37 682.24 5.33 357 117 158 41 685.12 5.88 358 117 161 44 680.15 6.38 359 117 162 45 677.38 7.76 360 120 161 41 790.53 0.47 361 121 161 40 790.57 0.48 362 122 161 39 790.57 0.49 363 122 207 85 251.09 25.59 364 123 161 38 790.62 0.48 365 123 162 39 790.58 0.48 366 123 163 40 790.57 0.48 367 124 163 39 790.58 0.48 368 124 165 41 790.50 0.48 369 124 209 85 250.41 25.89 370 124 232 108 119.75 0.55 371 125 150 25 790.91 0.49 372 125 163 38 790.62 0.48 373 125 164 39 790.58 0.48 374 125 165 40 790.57 0.48 375 126 165 39 790.58 0.48 376 126 167 41 790.52 0.48 377 126 232 106 120.00 0.56 378 127 143 16 791.14 0.48 379 127 146 19 791.04 0.49 380 127 147 20 791.03 0.49 381 127 150 23 791.00 0.50 382 127 164 37 790.67 0.48 383 127 165 38 790.63 0.48 384 127 166 39 790.58 0.48 385 127 167 40 790.57 0.48 386 127 190 63 285.22 21.15 387 128 143 15 791.19 0.49 388 128 146 18 791.11 0.51 389 128 166 38 790.60 0.48 390 128 167 39 790.58 0.48 391 128 169 41 790.48 0.48 392 129 133 4 830.49 6.44 393 129 134 5 823.47 2.94 394 129 139 10 791.37 0.49 395 129 142 13 791.23 0.50 396 129 143 14 791.25 0.49 397 129 166 37 790.63 0.48 398 129 169 40 790.51 0.49 399 129 170 41 790.49 0.48 400 130 133 3 860.08 0.30 401 130 134 4 825.36 2.02 402 130 169 39 790.52 0.49 403 130 170 40 790.47 0.49 404 131 134 3 850.66 5.01 405 131 135 4 830.69 0.64 406 131 170 39 790.48 0.49 407 131 174 43 729.30 1.52 408 131 176 45 706.15 2.54 409 132 135 3 854.05 3.33 410 132 136 4 861.56 0.43 411 133 136 3 868.83 3.20 412 133 137 4 861.56 0.43 413 133 138 5 791.43 0.51 414 133 142 9 791.37 0.49 415 134 137 3 868.83 3.20 416 134 139 5 791.40 0.52 417 134 166 32 790.74 0.48 418 134 170 36 790.55 0.50 419 135 176 41 757.88 3.12 420 138 141 3 870.42 4.53 421 138 142 4 842.77 0.04 422 139 142 3 863.47 1.57 423 139 143 4 845.09 1.12 424 140 143 3 861.21 0.45 425 140 144 4 841.98 0.70 426 140 189 49 285.36 21.14 427 141 144 3 875.65 0.65 428 141 145 4 855.49 3.29 429 142 145 3 867.77 3.29 430 142 146 4 861.22 0.43 431 143 146 3 861.63 0.23 432 143 147 4 861.28 0.46 433 143 166 23 790.67 0.49 434 143 189 46 285.31 21.15 435 143 190 47 285.26 21.16 436 144 147 3 869.26 4.45 437 144 148 4 861.28 0.46 438 144 189 45 285.32 21.14 439 144 193 49 285.07 21.16 440 145 148 3 861.29 0.46 441 145 149 4 861.28 0.46 442 146 149 3 874.62 2.52 443 146 150 4 858.33 1.93 444 147 150 3 865.89 1.84 445 147 151 4 846.34 1.36 446 147 159 12 791.08 0.45 447 147 164 17 790.72 0.48 448 147 190 43 285.20 21.13 449 147 193 46 285.05 21.15 450 147 194 47 284.91 21.16 451 148 151 3 849.05 2.71 452 148 152 4 850.24 3.31 453 148 193 45 285.10 21.12 454 149 152 3 866.06 4.29 455 150 153 3 892.10 9.71 456 150 154 4 860.48 1.06 457 151 154 3 866.35 1.87 458 151 159 8 791.35 0.57 459 151 193 42 285.10 21.12 460 151 198 47 266.27 18.54 461 154 158 4 849.51 3.55 462 154 159 5 849.50 3.56 463 154 162 8 790.98 0.36 464 155 158 3 868.01 2.74 465 155 159 4 863.65 4.91 466 156 159 3 883.93 5.21 467 156 198 42 256.99 23.18 468 156 201 45 255.37 23.96 469 156 202 46 255.09 23.84 470 157 160 3 891.58 1.40 471 158 161 3 888.86 3.26 472 159 162 3 846.27 1.82 473 159 194 35 284.08 21.13 474 159 198 39 256.97 23.16 475 159 202 43 251.91 25.38 476 159 206 47 251.47 25.59 477 162 206 44 251.44 25.59 478 163 207 44 251.01 25.62 479 163 209 46 250.42 25.89 480 164 190 26 284.97 21.21 481 164 206 42 251.48 25.59 482 164 207 43 251.15 25.58 483 164 208 44 250.97 25.62 484 164 209 45 250.42 25.89 485 165 209 44 250.41 25.89 486 165 232 67 119.94 0.55 487 166 186 20 285.26 21.17 488 166 187 21 285.19 21.18 489 166 190 24 285.14 21.18 490 166 208 42 251.11 25.59 491 166 209 43 250.96 25.64 492 166 210 44 250.40 25.89 493 166 211 45 250.34 25.87 494 167 211 44 249.91 25.94 495 168 171 3 873.33 1.71 496 168 172 4 851.80 2.47 497 168 210 42 250.96 25.63 498 168 211 43 250.40 25.89 499 168 212 44 249.97 25.91 500 169 172 3 855.28 0.72 501 170 174 4 824.84 11.03 502 170 176 6 540.23 28.64 503 170 178 8 286.12 21.21 504 170 182 12 285.89 21.19 505 171 174 3 862.28 0.52 506 171 176 5 292.33 18.04 507 171 178 7 286.00 21.18 508 172 212 40 249.80 25.78 509 177 182 5 852.98 3.04 510 178 182 4 836.43 5.24 511 178 210 32 251.44 25.57 512 179 182 3 855.35 0.40 513 179 183 4 846.85 0.03 514 180 183 3 854.24 2.77 515 180 184 4 846.86 0.03 516 180 221 41 174.87 26.15 517 180 222 42 173.35 25.41 518 180 225 45 122.11 0.21 519 181 184 3 858.34 0.96 520 181 185 4 849.41 1.24 521 182 185 3 853.64 0.17 522 182 186 4 850.59 0.66 523 183 186 3 858.65 0.79 524 183 187 4 847.93 0.67 525 183 210 27 251.44 25.57 526 183 222 39 173.47 25.34 527 184 187 3 855.95 3.33 528 184 188 4 848.63 0.74 529 184 225 41 121.70 0.28 530 184 226 42 121.72 0.28 531 184 227 43 121.67 0.29 532 185 188 3 855.32 1.89 533 185 189 4 849.38 5.43 534 186 189 3 857.70 1.28 535 186 190 4 857.69 1.28 536 187 190 3 858.70 0.77 537 187 191 4 849.07 1.27 538 187 208 21 251.59 25.57 539 187 209 22 251.59 25.58 540 187 210 23 251.57 25.57 541 187 227 40 121.39 0.25 542 187 228 41 121.39 0.25 543 188 191 3 860.11 2.83 544 188 192 4 849.07 1.26 545 188 227 39 121.37 0.21 546 189 192 3 870.75 0.82 547 189 193 4 849.81 0.89 548 190 193 3 866.54 1.29 549 190 194 4 851.13 1.55 550 190 208 18 251.62 25.57 551 191 194 3 865.94 1.59 552 191 195 4 831.81 2.49 553 191 203 12 252.59 25.34 554 191 206 15 252.18 25.54 555 191 208 17 251.83 25.46 556 191 227 36 121.34 0.22 557 191 229 38 121.30 0.23 558 192 195 3 840.92 6.66 559 192 196 4 818.93 3.96 560 193 196 3 862.98 4.37 561 193 197 4 837.69 5.43 562 193 198 5 818.89 3.97 563 194 197 3 859.45 7.04 564 194 198 4 804.64 2.94 565 194 199 5 804.64 2.94 566 194 202 8 308.18 22.98 567 194 203 9 266.28 32.12 568 194 206 12 252.18 25.54 569 195 199 4 598.03 100.37 570 195 200 5 307.51 23.34 571 195 203 8 266.28 32.12 572 198 202 4 842.27 1.80 573 199 202 3 853.09 3.04 574 199 203 4 850.41 0.70 575 200 203 3 858.25 1.70 576 200 204 4 850.41 0.70 577 201 204 3 871.59 3.72 578 201 205 4 858.25 1.70 579 202 205 3 859.73 0.96 580 202 206 4 851.93 1.46 581 203 206 3 860.53 0.56 582 206 229 23 121.06 0.30 583 207 229 22 121.01 0.29 584 208 228 20 121.26 0.26 585 208 229 21 121.09 0.32 586 209 229 20 121.20 0.28 587 209 230 21 121.01 0.29 588 209 231 22 120.75 0.21 589 209 232 23 120.12 0.51 590 210 214 4 894.38 4.27 591 210 222 12 121.19 0.33 592 210 228 18 121.37 0.28 593 210 230 20 121.20 0.28 594 210 231 21 120.78 0.20 595 210 232 22 120.78 0.20 596 211 232 21 120.73 0.22 597 213 218 5 827.82 6.64 598 214 218 4 811.60 1.48 599 214 219 5 328.31 25.39 600 214 222 8 316.23 19.64 601 214 231 17 121.42 0.27 602 214 233 19 121.23 0.31 603 214 236 22 120.70 0.22 604 214 245 31 52.86 1.51 605 215 218 3 866.20 2.06 606 215 219 4 754.24 30.14 607 215 241 26 61.05 1.10 608 215 245 30 53.77 1.96 609 216 220 4 874.62 2.31 610 216 241 25 58.88 0.03 611 216 244 28 54.34 2.24 612 216 245 29 53.77 1.97 613 217 241 24 67.58 1.23 614 218 221 3 865.81 2.10 615 218 222 4 834.31 4.45 616 219 222 3 849.28 1.20 617 219 223 4 834.30 4.45 618 219 231 12 121.75 0.33 619 219 245 26 49.69 0.32 620 219 248 29 41.30 1.51 621 220 223 3 851.86 3.89 622 220 224 4 834.73 4.67 623 220 248 28 41.30 1.51 624 221 224 3 840.72 1.68 625 221 225 4 853.29 4.61 626 222 225 3 884.56 4.21 627 222 228 6 296.50 19.50 628 222 231 9 125.26 1.34 629 223 228 5 229.06 53.22 630 223 229 6 125.31 1.33 631 223 230 7 125.27 1.33 632 223 231 8 125.25 1.32 633 223 248 25 38.81 2.73 634 223 249 26 38.26 2.83 635 223 250 27 38.28 2.85 636 225 228 3 855.44 2.30 637 228 231 3 1054.44 0.72 638 230 249 19 25.97 0.02 639 231 245 14 48.28 0.42 640 231 249 18 39.48 0.82 641 233 236 3 895.49 2.56 642 233 237 4 826.97 1.17 643 233 242 9 58.83 0.01 644 233 245 12 52.97 0.97 645 234 237 3 882.02 0.76 646 235 238 3 873.00 0.78 647 235 239 4 866.12 3.06 648 236 239 3 872.47 0.98 649 236 241 5 295.78 11.74 650 236 242 6 226.59 5.00 651 236 245 9 172.41 22.07 652 237 242 5 226.58 5.00 653 239 242 3 875.09 0.65 654 240 243 3 884.15 3.89 655 241 244 3 865.25 2.43 656 241 245 4 849.73 1.81 657 242 245 3 847.44 0.67 658 242 246 4 849.25 0.24 659 243 246 3 857.72 1.06 660 243 247 4 853.05 1.12 661 244 247 3 867.02 3.40 662 244 248 4 845.88 1.96 663 245 248 3 864.76 4.09 664 245 249 4 864.63 4.15 665 246 249 3 881.16 1.74 666 247 250 3 873.43 0.24