BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 114 112 27.05 1.11 2 2 136 134 27.14 1.15 3 2 137 135 27.23 1.09 4 2 138 136 27.39 1.15 5 2 141 139 27.38 1.15 6 3 134 131 27.39 1.15 7 3 135 132 27.39 1.15 8 3 136 133 27.39 1.15 9 3 137 134 27.39 1.15 10 3 138 135 27.59 1.05 11 3 168 165 27.72 1.12 12 5 9 4 803.28 0.88 13 5 10 5 709.52 40.23 14 5 13 8 710.36 39.81 15 5 136 131 33.17 0.89 16 6 9 3 863.71 0.80 17 6 10 4 805.18 0.10 18 7 10 3 850.85 5.63 19 7 11 4 825.32 0.73 20 8 11 3 824.18 1.31 21 8 12 4 824.17 1.31 22 8 57 49 46.02 0.17 23 8 88 80 46.44 0.27 24 8 89 81 46.90 0.49 25 8 112 104 46.96 0.51 26 8 184 176 46.90 0.51 27 8 187 179 46.90 0.52 28 9 12 3 835.45 2.61 29 9 13 4 820.75 3.02 30 9 112 103 46.95 0.52 31 9 135 126 47.04 0.54 32 9 136 127 47.04 0.54 33 9 184 175 46.90 0.50 34 9 185 176 46.95 0.51 35 10 13 3 829.40 1.74 36 10 14 4 815.61 0.84 37 11 14 3 833.73 3.47 38 11 15 4 831.21 6.96 39 11 88 77 49.27 0.65 40 12 15 3 836.45 2.12 41 12 16 4 838.09 1.30 42 12 85 73 50.66 0.36 43 12 88 76 49.27 0.65 44 12 112 100 49.09 0.44 45 13 16 3 827.27 5.12 46 13 17 4 826.26 4.61 47 13 114 101 61.41 0.38 48 13 136 123 56.25 0.86 49 14 17 3 849.64 4.42 50 14 18 4 838.13 1.32 51 15 18 3 838.28 1.26 52 15 19 4 804.33 0.28 53 15 85 70 64.78 0.44 54 15 87 72 63.41 0.12 55 16 19 3 809.30 2.07 56 16 112 96 65.60 0.82 57 16 114 98 67.94 1.22 58 16 115 99 68.45 1.44 59 16 118 102 67.96 1.19 60 17 114 97 68.35 1.42 61 17 115 98 69.47 1.94 62 18 115 97 71.86 2.40 63 19 84 65 69.74 2.08 64 19 85 66 69.53 1.98 65 19 86 67 69.75 2.08 66 19 93 74 78.02 1.41 67 19 111 92 69.89 2.14 68 19 115 96 79.20 1.26 69 21 117 96 81.60 2.22 70 21 121 100 81.05 1.95 71 22 93 71 81.11 1.89 72 22 97 75 84.98 1.21 73 22 121 99 81.70 2.17 74 22 124 102 91.45 0.30 75 22 125 103 81.72 2.18 76 24 97 73 99.34 1.30 77 24 124 100 106.28 3.90 78 26 29 3 952.09 0.54 79 26 30 4 895.63 4.61 80 26 123 97 136.52 0.15 81 26 124 98 136.16 0.21 82 26 126 100 136.13 0.23 83 26 127 101 136.62 0.21 84 26 128 102 138.81 0.12 85 28 100 72 137.65 0.71 86 28 103 75 139.81 1.00 87 28 105 77 145.05 0.11 88 28 107 79 145.59 0.17 89 28 126 98 139.16 1.34 90 28 128 100 145.61 0.17 91 30 123 93 158.65 0.47 92 30 128 98 147.18 0.96 93 30 152 122 159.60 0.42 94 30 153 123 159.62 0.42 95 30 154 124 159.52 0.39 96 31 104 73 158.66 0.48 97 31 106 75 158.65 0.48 98 31 128 97 159.26 0.47 99 32 35 3 844.40 8.05 100 32 36 4 816.38 0.19 101 32 106 74 159.41 0.42 102 33 36 3 827.62 5.82 103 33 37 4 816.38 0.19 104 33 49 16 159.49 0.39 105 33 78 45 159.53 0.41 106 33 79 46 159.42 0.43 107 33 106 73 159.53 0.41 108 33 108 75 159.53 0.41 109 33 129 96 159.59 0.42 110 33 176 143 168.15 1.14 111 34 37 3 820.48 2.24 112 34 38 4 816.37 0.19 113 34 155 121 168.19 1.16 114 34 176 142 168.13 1.14 115 34 206 172 168.43 1.25 116 35 38 3 822.96 0.26 117 35 39 4 820.10 1.67 118 36 39 3 838.61 0.80 119 36 40 4 817.82 2.81 120 36 77 41 168.72 1.33 121 36 78 42 168.68 1.33 122 36 105 69 168.68 1.33 123 36 106 70 168.65 1.32 124 37 40 3 831.81 4.20 125 37 41 4 805.58 3.28 126 37 47 10 168.72 1.33 127 37 49 12 168.72 1.33 128 37 78 41 168.72 1.33 129 37 176 139 169.27 1.25 130 37 206 169 169.25 1.24 131 37 208 171 168.80 1.32 132 37 228 191 168.85 1.30 133 37 229 192 168.75 1.32 134 38 41 3 815.47 0.60 135 38 42 4 805.27 3.12 136 38 206 168 169.27 1.26 137 38 228 190 169.15 1.24 138 39 42 3 814.87 0.03 139 39 43 4 803.56 2.25 140 40 43 3 828.06 5.08 141 40 44 4 803.38 2.35 142 40 45 5 714.19 40.41 143 40 47 7 168.83 1.31 144 40 78 38 168.83 1.31 145 41 44 3 810.72 3.04 146 41 223 182 169.84 1.55 147 41 228 187 169.84 1.54 148 46 219 173 226.99 0.21 149 46 230 184 227.60 0.29 150 46 246 200 115.47 1.39 151 46 247 201 106.72 0.14 152 47 78 31 533.08 30.36 153 47 228 181 228.04 0.45 154 47 229 182 227.62 0.29 155 47 230 183 227.61 0.29 156 48 77 29 533.14 30.38 157 48 78 30 533.14 30.38 158 48 79 31 533.10 30.36 159 48 219 171 227.64 0.28 160 48 230 182 227.64 0.28 161 48 232 184 227.58 0.28 162 48 236 188 210.46 0.17 163 48 243 195 121.84 0.20 164 48 246 198 115.49 1.40 165 48 247 199 107.54 0.56 166 49 78 29 533.15 30.38 167 49 79 30 533.13 30.37 168 49 80 31 533.08 30.36 169 49 81 32 533.07 30.36 170 49 208 159 229.25 0.10 171 49 229 180 229.25 0.10 172 49 230 181 227.68 0.28 173 49 231 182 227.64 0.28 174 49 232 183 227.64 0.28 175 50 73 23 533.28 30.36 176 50 77 27 533.22 30.36 177 50 79 29 533.15 30.37 178 50 80 30 533.10 30.37 179 50 81 31 533.09 30.36 180 50 232 182 227.67 0.27 181 50 236 186 210.77 0.01 182 51 69 18 533.38 30.34 183 51 81 30 533.09 30.36 184 51 83 32 533.08 30.36 185 51 110 59 237.00 0.51 186 51 231 180 229.26 0.10 187 51 232 181 228.67 0.21 188 51 233 182 227.58 0.28 189 51 237 186 210.77 0.01 190 52 64 12 533.50 30.39 191 52 66 14 533.31 30.30 192 52 69 17 533.37 30.34 193 52 81 29 533.15 30.37 194 52 82 30 533.10 30.36 195 52 83 31 533.08 30.36 196 52 95 43 241.06 0.15 197 52 237 185 213.33 0.54 198 53 56 3 904.10 0.96 199 53 60 7 533.86 30.36 200 53 83 30 533.10 30.35 201 53 89 36 387.78 3.83 202 53 233 180 229.22 0.11 203 53 234 181 228.97 0.20 204 53 237 184 218.16 1.13 205 54 61 7 533.79 30.36 206 54 62 8 533.69 30.36 207 54 66 12 533.53 30.37 208 54 90 36 387.81 3.85 209 55 60 5 533.88 30.37 210 55 61 6 533.79 30.36 211 55 88 33 523.88 27.40 212 55 89 34 468.41 25.94 213 55 90 35 387.88 3.85 214 56 59 3 836.79 1.30 215 56 60 4 533.89 30.37 216 56 88 32 523.92 27.40 217 56 89 33 512.28 21.93 218 57 88 31 529.12 29.99 219 57 89 32 527.98 29.42 220 57 187 130 231.34 0.85 221 58 182 124 231.50 0.81 222 58 187 129 231.28 0.89 223 60 234 174 229.65 0.03 224 60 237 177 228.96 0.21 225 60 238 178 228.37 0.43 226 62 237 175 227.87 0.18 227 62 238 176 227.62 0.29 228 64 69 5 801.49 2.80 229 64 237 173 227.61 0.29 230 64 238 174 227.50 0.24 231 64 240 176 219.09 2.33 232 65 68 3 825.68 0.27 233 65 69 4 805.05 2.50 234 66 69 3 829.07 1.97 235 66 70 4 818.39 3.91 236 66 95 29 241.06 0.15 237 66 99 33 240.96 0.13 238 67 70 3 831.29 5.00 239 67 71 4 822.06 5.47 240 67 98 31 241.03 0.15 241 67 99 32 240.87 0.09 242 67 102 35 240.72 0.04 243 68 71 3 836.21 2.54 244 68 72 4 815.61 2.25 245 69 72 3 824.64 3.08 246 69 73 4 821.09 0.49 247 69 236 167 227.50 0.24 248 69 237 168 225.43 0.30 249 69 240 171 123.83 0.58 250 70 73 3 830.12 0.35 251 70 74 4 822.25 0.08 252 70 80 10 533.21 30.39 253 70 99 29 240.77 0.09 254 70 103 33 240.42 0.01 255 71 74 3 833.88 2.21 256 71 75 4 836.34 3.45 257 71 102 31 240.50 0.02 258 71 103 32 240.44 0.01 259 72 75 3 843.57 0.17 260 72 76 4 840.32 1.46 261 72 240 168 123.84 0.58 262 72 241 169 123.83 0.58 263 72 243 171 122.38 0.01 264 72 244 172 122.41 0.10 265 73 76 3 840.42 1.41 266 73 77 4 806.07 2.39 267 73 80 7 533.39 30.32 268 73 236 163 226.49 0.10 269 73 240 167 123.84 0.58 270 73 243 170 121.83 0.20 271 74 103 29 240.36 0.00 272 74 104 30 240.34 0.01 273 74 105 31 239.55 0.04 274 76 243 167 121.84 0.20 275 76 244 168 122.42 0.09 276 76 247 171 107.79 0.62 277 77 105 28 238.59 0.45 278 78 106 28 238.34 0.36 279 79 105 26 238.66 0.51 280 79 106 27 238.31 0.37 281 80 99 19 240.76 0.09 282 80 103 23 240.34 0.01 283 80 105 25 239.72 0.01 284 80 106 26 238.34 0.36 285 80 107 27 238.11 0.42 286 80 108 28 237.98 0.47 287 81 108 27 237.97 0.46 288 81 110 29 237.06 0.47 289 81 231 150 229.22 0.11 290 82 95 13 240.99 0.17 291 82 96 14 240.93 0.14 292 82 99 17 240.89 0.12 293 82 107 25 238.63 0.49 294 82 108 26 238.24 0.41 295 82 109 27 237.93 0.45 296 82 110 28 237.56 0.32 297 83 89 6 387.79 3.83 298 83 91 8 384.96 4.49 299 83 110 27 237.75 0.42 300 84 91 7 386.56 3.70 301 84 92 8 307.62 29.69 302 84 93 9 241.26 0.06 303 84 96 12 241.11 0.14 304 84 109 25 238.23 0.40 305 84 110 26 237.91 0.45 306 84 111 27 237.03 0.46 307 85 88 3 874.65 0.30 308 85 89 4 666.55 19.87 309 85 90 5 439.84 29.84 310 85 91 6 387.66 3.88 311 85 111 26 237.27 0.65 312 85 112 27 236.87 0.45 313 86 90 4 387.88 3.86 314 86 91 5 387.88 3.86 315 86 92 6 387.79 3.82 316 89 112 23 237.27 0.65 317 92 95 3 834.94 2.23 318 92 96 4 816.56 3.65 319 93 96 3 827.12 6.12 320 93 97 4 808.18 1.86 321 93 109 16 238.84 0.41 322 93 121 28 238.44 1.32 323 93 125 32 238.45 1.32 324 94 97 3 829.49 0.56 325 94 98 4 808.18 1.86 326 95 98 3 839.94 1.53 327 95 99 4 816.27 2.17 328 96 99 3 817.73 1.45 329 96 100 4 816.26 2.18 330 96 107 11 240.91 0.57 331 96 109 13 238.28 0.39 332 96 125 29 240.07 1.08 333 96 127 31 240.93 0.61 334 97 100 3 821.61 3.39 335 97 101 4 816.99 1.81 336 97 125 28 239.15 1.53 337 98 101 3 833.32 2.41 338 98 102 4 811.80 0.78 339 99 102 3 828.83 4.65 340 99 103 4 811.80 0.78 341 99 107 8 241.09 0.56 342 100 103 3 842.26 0.19 343 100 107 7 241.16 0.59 344 100 125 25 314.88 9.96 345 100 126 26 241.10 0.67 346 103 107 4 859.10 2.74 347 106 128 22 332.44 15.06 348 107 127 20 332.73 14.97 349 107 128 21 332.68 15.00 350 107 129 22 332.73 14.97 351 108 129 21 332.73 14.97 352 108 130 22 332.73 14.97 353 108 131 23 332.72 14.96 354 108 157 49 332.50 15.03 355 108 231 123 301.89 3.62 356 109 113 4 890.10 2.70 357 109 118 9 345.06 13.54 358 109 122 13 332.88 14.97 359 109 127 18 332.86 14.98 360 109 129 20 332.78 14.96 361 109 130 21 332.75 14.97 362 109 131 22 332.75 14.96 363 110 131 21 332.78 14.96 364 110 210 100 332.75 14.96 365 110 231 121 308.54 3.18 366 111 133 22 344.94 13.59 367 112 118 6 784.95 5.95 368 112 133 21 344.94 13.59 369 112 136 24 345.00 13.58 370 112 184 72 344.81 13.53 371 113 118 5 600.82 1.36 372 113 119 6 600.82 1.36 373 113 130 17 344.99 13.58 374 113 131 18 344.99 13.58 375 113 132 19 344.98 13.58 376 113 137 24 345.20 13.58 377 113 145 32 345.70 13.73 378 114 118 4 836.64 2.88 379 114 136 22 345.20 13.58 380 114 137 23 345.20 13.58 381 114 145 31 345.80 13.69 382 115 118 3 842.14 3.77 383 115 145 30 358.84 7.17 384 116 119 3 838.15 2.83 385 116 120 4 819.37 0.01 386 116 145 29 370.80 13.15 387 116 148 32 454.51 27.99 388 117 120 3 835.64 3.38 389 117 121 4 819.52 0.07 390 118 121 3 824.27 2.31 391 118 122 4 819.50 0.07 392 119 122 3 824.28 2.31 393 119 123 4 817.11 1.28 394 119 130 11 456.22 27.14 395 119 145 26 548.94 29.46 396 119 148 29 549.01 29.46 397 119 149 30 549.00 29.47 398 119 152 33 550.50 28.75 399 119 154 35 549.24 29.36 400 120 123 3 824.28 2.31 401 120 124 4 815.52 0.49 402 120 148 28 550.01 28.96 403 120 152 32 563.50 34.00 404 121 124 3 822.64 4.07 405 121 125 4 814.56 2.44 406 122 125 3 830.70 0.04 407 122 126 4 816.95 1.25 408 122 127 5 611.03 4.71 409 122 130 8 458.22 27.96 410 122 154 32 677.91 12.88 411 123 126 3 822.62 4.08 412 123 152 29 681.44 14.63 413 123 154 31 678.68 13.28 414 127 154 27 772.13 0.08 415 128 154 26 772.13 0.08 416 129 154 25 772.14 0.08 417 129 155 26 772.13 0.08 418 129 156 27 772.09 0.09 419 130 149 19 772.26 0.08 420 130 154 24 772.25 0.07 421 130 156 26 772.10 0.09 422 130 157 27 772.09 0.09 423 131 157 26 772.12 0.07 424 131 159 28 772.04 0.09 425 132 137 5 801.77 7.00 426 132 142 10 772.35 0.09 427 132 145 13 772.36 0.08 428 132 146 14 772.35 0.09 429 132 156 24 772.27 0.05 430 132 157 25 772.22 0.04 431 132 158 26 772.08 0.07 432 132 159 27 772.08 0.07 433 133 136 3 838.52 0.81 434 133 137 4 811.84 2.01 435 133 159 26 772.09 0.07 436 133 184 51 543.78 27.65 437 134 137 3 829.27 4.21 438 134 142 8 772.43 0.07 439 134 158 24 772.27 0.05 440 134 159 25 772.16 0.04 441 134 160 26 772.12 0.07 442 134 165 31 700.84 1.74 443 134 168 34 699.45 1.45 444 134 169 35 699.39 1.46 445 135 161 26 772.11 0.06 446 135 165 30 700.88 1.74 447 135 183 48 664.36 0.70 448 135 184 49 560.43 20.16 449 137 141 4 797.91 1.69 450 137 142 5 785.93 5.58 451 137 145 8 774.11 0.42 452 138 141 3 837.08 1.40 453 138 142 4 819.38 7.46 454 139 142 3 833.37 1.33 455 139 143 4 828.42 2.94 456 139 168 29 699.47 1.47 457 139 171 32 699.48 1.47 458 139 172 33 699.27 1.50 459 140 143 3 836.82 0.40 460 140 144 4 828.42 2.94 461 141 144 3 835.81 0.75 462 141 145 4 823.71 3.10 463 142 145 3 841.14 2.63 464 142 146 4 811.35 1.23 465 142 158 16 772.32 0.07 466 142 168 26 699.47 1.47 467 142 172 30 698.90 1.48 468 143 146 3 838.39 4.04 469 143 147 4 813.12 1.45 470 143 172 29 698.86 1.48 471 144 147 3 823.34 2.10 472 144 148 4 810.08 0.60 473 145 148 3 815.08 1.89 474 145 149 4 815.17 1.94 475 146 149 3 836.77 0.92 476 146 150 4 828.93 4.84 477 146 156 10 772.58 0.04 478 146 158 12 772.36 0.07 479 146 172 26 698.85 1.47 480 147 150 3 831.73 2.60 481 147 151 4 822.57 2.00 482 148 151 3 825.06 0.76 483 148 152 4 825.08 0.74 484 149 152 3 842.30 0.76 485 149 153 4 822.57 2.00 486 149 154 5 790.50 8.88 487 149 156 7 772.62 0.03 488 150 153 3 822.56 2.00 489 150 154 4 800.52 3.87 490 150 155 5 788.31 7.77 491 150 156 6 788.31 7.77 492 150 173 23 698.78 1.45 493 150 175 25 698.56 1.44 494 155 175 20 698.49 1.42 495 155 176 21 698.41 1.40 496 156 176 20 698.48 1.43 497 157 176 19 698.48 1.42 498 157 177 20 698.40 1.41 499 157 178 21 698.36 1.43 500 157 208 51 539.07 27.50 501 157 231 74 310.56 2.69 502 158 169 11 698.90 1.49 503 158 172 14 698.90 1.48 504 158 174 16 698.62 1.42 505 158 176 18 698.54 1.44 506 158 177 19 698.41 1.41 507 158 178 20 698.37 1.43 508 159 178 19 698.36 1.43 509 159 180 21 698.34 1.44 510 159 184 25 566.71 26.19 511 159 210 51 539.03 27.50 512 160 165 5 699.54 1.46 513 160 166 6 699.38 1.45 514 160 169 9 699.39 1.45 515 160 177 17 698.55 1.45 516 160 178 18 698.48 1.43 517 160 179 19 698.37 1.43 518 160 180 20 698.37 1.43 519 160 197 37 542.58 27.95 520 160 201 41 540.10 27.63 521 161 165 4 811.43 1.88 522 161 180 19 698.36 1.43 523 161 182 21 698.33 1.42 524 161 183 22 680.11 0.21 525 161 193 32 542.66 27.98 526 161 197 36 542.60 27.96 527 162 165 3 823.25 5.79 528 162 166 4 812.66 1.29 529 162 197 35 542.60 27.96 530 163 166 3 819.08 4.50 531 163 167 4 812.23 1.52 532 163 196 33 542.66 27.98 533 163 197 34 542.60 27.96 534 163 200 37 542.02 27.82 535 164 167 3 827.33 6.21 536 164 168 4 812.23 1.52 537 165 168 3 822.47 5.70 538 165 169 4 822.00 6.42 539 166 169 3 821.09 6.88 540 166 170 4 821.09 6.88 541 166 177 11 698.70 1.41 542 166 197 31 542.53 27.96 543 166 200 34 541.91 27.78 544 166 201 35 540.07 27.51 545 167 170 3 837.50 1.30 546 167 171 4 820.31 7.27 547 168 171 3 831.61 1.62 548 168 172 4 821.41 6.72 549 169 172 3 848.11 6.62 550 169 173 4 810.85 2.69 551 169 174 5 704.80 4.40 552 169 177 8 698.68 1.41 553 170 173 3 829.52 2.67 554 170 177 7 698.88 1.40 555 170 202 32 539.54 27.54 556 174 177 3 876.61 4.94 557 174 205 31 539.29 27.55 558 175 205 30 539.28 27.55 559 175 206 31 539.28 27.55 560 176 205 29 539.30 27.55 561 176 206 30 539.27 27.55 562 176 208 32 539.21 27.57 563 177 201 24 539.54 27.54 564 177 205 28 539.35 27.54 565 177 206 29 539.28 27.55 566 177 207 30 539.22 27.56 567 177 208 31 539.18 27.55 568 178 208 30 539.08 27.50 569 178 210 32 539.00 27.53 570 179 194 15 542.57 27.96 571 179 197 18 542.12 27.76 572 179 198 19 541.89 27.80 573 179 201 22 540.14 27.47 574 179 207 28 539.29 27.54 575 179 208 29 539.26 27.55 576 179 209 30 539.17 27.55 577 179 210 31 539.06 27.50 578 180 184 4 843.63 2.70 579 180 210 30 539.06 27.50 580 180 211 31 539.06 27.50 581 181 189 8 542.93 27.91 582 181 190 9 542.89 27.89 583 181 191 10 542.79 27.89 584 181 194 13 542.59 27.96 585 181 210 29 539.09 27.50 586 181 211 30 539.07 27.50 587 182 187 5 543.40 27.70 588 182 188 6 542.98 27.90 589 182 189 7 542.93 27.91 590 182 211 29 539.02 27.51 591 183 186 3 841.43 1.16 592 183 187 4 617.88 57.86 593 183 188 5 542.98 27.91 594 184 187 3 811.23 2.38 595 189 212 23 538.95 27.47 596 190 193 3 842.95 7.37 597 190 194 4 817.95 4.67 598 191 194 3 823.51 1.90 599 191 195 4 823.51 1.90 600 191 212 21 539.22 27.53 601 191 218 27 496.00 27.55 602 192 195 3 827.60 0.14 603 192 196 4 811.05 1.24 604 193 196 3 829.35 1.02 605 193 197 4 813.40 0.06 606 194 197 3 828.54 1.03 607 194 198 4 816.48 3.21 608 194 209 15 539.36 27.54 609 194 210 16 539.34 27.54 610 195 198 3 836.29 2.50 611 195 199 4 816.47 3.21 612 195 218 23 484.53 24.20 613 195 221 26 345.22 2.19 614 195 222 27 330.70 6.60 615 195 225 30 314.01 1.68 616 196 199 3 840.44 0.43 617 196 200 4 816.49 3.21 618 197 200 3 831.24 4.17 619 197 201 4 814.18 4.37 620 198 201 3 842.59 0.64 621 198 207 9 539.41 27.54 622 198 222 24 314.03 1.67 623 198 225 27 314.00 1.68 624 198 227 29 313.29 1.57 625 201 205 4 819.59 5.23 626 201 207 6 540.99 26.75 627 202 205 3 849.05 0.48 628 202 207 5 826.48 1.78 629 203 207 4 852.68 0.40 630 206 228 22 312.91 1.70 631 207 226 19 313.31 1.66 632 207 227 20 313.00 1.70 633 207 228 21 312.94 1.71 634 207 229 22 312.88 1.72 635 208 227 19 313.03 1.71 636 208 229 21 312.78 1.72 637 208 230 22 312.53 1.82 638 208 231 23 312.53 1.82 639 209 213 4 849.87 0.85 640 209 222 13 313.44 1.68 641 209 227 18 313.06 1.71 642 209 229 20 312.89 1.76 643 209 230 21 312.53 1.82 644 209 231 22 310.66 2.75 645 210 231 21 310.62 2.74 646 213 218 5 693.78 44.20 647 213 219 6 499.01 4.08 648 213 222 9 381.60 21.36 649 213 230 17 312.70 1.90 650 213 232 19 310.63 2.75 651 213 235 22 310.57 2.75 652 214 235 21 310.57 2.75 653 214 242 28 126.37 0.64 654 214 246 32 123.05 0.33 655 215 218 3 835.12 1.48 656 215 219 4 828.99 4.54 657 215 246 31 122.56 0.05 658 216 219 3 831.61 3.23 659 216 220 4 825.36 6.36 660 216 245 29 123.57 0.58 661 216 246 30 122.44 0.10 662 216 248 32 122.41 0.09 663 217 220 3 838.21 0.07 664 217 221 4 812.65 1.44 665 218 221 3 838.20 6.53 666 218 222 4 815.55 1.66 667 219 222 3 828.94 3.62 668 219 223 4 815.55 1.67 669 219 230 11 313.76 1.74 670 219 232 13 313.48 1.60 671 219 246 27 121.59 0.17 672 220 223 3 830.69 2.75 673 220 224 4 827.06 0.93 674 221 224 3 833.52 1.81 675 221 225 4 827.06 0.93 676 222 225 3 831.33 2.43 677 222 226 4 827.05 0.93 678 222 227 5 798.41 3.73 679 222 230 8 555.28 23.44 680 223 226 3 830.06 0.68 681 223 227 4 801.49 5.26 682 223 228 5 785.49 2.73 683 223 230 7 696.50 44.79 684 227 230 3 931.64 0.82 685 232 235 3 882.12 3.66 686 232 236 4 817.25 4.08 687 232 246 14 119.44 0.03 688 233 236 3 833.75 1.15 689 233 237 4 803.89 8.63 690 234 237 3 833.75 1.15 691 234 238 4 803.89 8.63 692 235 238 3 828.57 3.70 693 235 239 4 803.18 8.99 694 235 246 11 320.88 1.14 695 236 239 3 798.25 11.43 696 236 240 4 662.78 63.17 697 236 242 6 325.79 3.59 698 236 243 7 320.20 0.80 699 236 246 10 320.20 0.80 700 239 242 3 842.64 2.60 701 240 243 3 860.25 3.79 702 241 244 3 846.07 4.84 703 242 245 3 828.52 1.62 704 242 246 4 826.62 0.67 705 243 246 3 828.75 0.40 706 243 247 4 826.62 0.68 707 244 247 3 844.44 3.29 708 244 248 4 824.33 1.80 709 245 248 3 837.18 4.61