BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 8 109 101 425.18 0.06 2 8 110 102 425.03 0.01 3 8 113 105 425.02 0.00 4 8 121 113 424.66 0.01 5 8 122 114 424.60 0.03 6 8 127 119 424.49 0.07 7 8 131 123 423.72 0.20 8 9 107 98 426.34 0.21 9 9 108 99 425.98 0.10 10 9 109 100 425.43 0.01 11 9 122 113 424.66 0.01 12 9 131 122 423.73 0.19 13 10 107 97 426.42 0.21 14 10 108 98 426.06 0.10 15 10 131 121 423.72 0.20 16 10 132 122 423.72 0.20 17 11 54 43 587.38 1.53 18 11 55 44 587.35 1.53 19 11 56 45 587.32 1.52 20 11 57 46 587.28 1.50 21 11 75 64 586.53 1.44 22 11 76 65 586.23 1.56 23 11 77 66 585.56 1.54 24 11 107 96 426.43 0.21 25 11 108 97 426.35 0.21 26 11 131 120 424.46 0.07 27 11 132 121 424.22 0.07 28 11 133 122 424.31 0.02 29 12 75 63 586.97 1.48 30 12 76 64 586.51 1.44 31 12 77 65 586.27 1.54 32 12 78 66 585.68 1.59 33 12 79 67 585.65 1.59 34 12 82 70 585.56 1.55 35 12 106 94 426.88 0.26 36 12 107 95 426.44 0.22 37 13 73 60 587.32 1.47 38 13 74 61 587.17 1.45 39 13 75 62 587.02 1.46 40 13 82 69 585.60 1.54 41 13 105 92 427.22 0.40 42 13 106 93 427.14 0.39 43 13 187 174 202.72 3.19 44 14 72 58 587.87 1.52 45 14 73 59 587.61 1.61 46 14 74 60 587.28 1.48 47 14 82 68 585.69 1.58 48 14 86 72 585.16 1.50 49 14 104 90 427.26 0.40 50 14 105 91 427.22 0.40 51 15 71 56 588.04 1.49 52 15 72 57 587.93 1.54 53 15 73 58 587.62 1.60 54 15 103 88 427.34 0.40 55 15 104 89 427.31 0.40 56 15 143 128 216.62 0.45 57 15 200 185 195.69 0.33 58 16 71 55 588.07 1.50 59 16 72 56 587.93 1.54 60 17 63 46 589.20 1.49 61 17 68 51 589.39 1.43 62 17 70 53 588.38 1.49 63 17 71 54 588.07 1.50 64 17 101 84 437.22 4.42 65 17 102 85 428.80 0.24 66 17 143 126 299.67 3.20 67 17 232 215 68.36 0.00 68 18 68 50 589.41 1.43 69 18 69 51 589.38 1.43 70 18 70 52 589.00 1.59 71 18 72 54 588.45 1.41 72 18 89 71 585.58 1.40 73 18 90 72 585.27 1.46 74 18 95 77 543.63 1.27 75 18 98 80 527.69 3.29 76 18 99 81 527.56 3.23 77 18 100 82 509.96 1.14 78 18 101 83 451.35 2.29 79 19 68 49 589.44 1.43 80 19 69 50 589.40 1.42 81 19 97 78 543.58 1.27 82 19 98 79 542.19 0.76 83 19 99 80 530.12 2.06 84 19 101 82 509.98 1.15 85 20 69 49 589.44 1.43 86 20 97 77 543.58 1.27 87 20 98 78 543.50 1.25 88 20 245 225 34.72 0.45 89 20 246 226 32.92 0.98 90 21 24 3 819.11 5.60 91 21 25 4 794.02 0.56 92 21 97 76 543.58 1.27 93 21 99 78 543.56 1.27 94 22 25 3 804.29 1.80 95 22 26 4 794.07 0.54 96 22 245 223 39.38 0.61 97 22 246 224 34.49 0.33 98 22 248 226 32.36 1.27 99 23 26 3 806.35 0.78 100 23 27 4 804.31 1.79 101 24 27 3 800.52 0.10 102 24 28 4 800.51 0.10 103 24 97 73 575.63 1.29 104 25 28 3 806.00 1.16 105 25 33 8 744.68 0.04 106 25 36 11 699.84 4.82 107 25 37 12 699.98 4.75 108 25 94 69 582.06 0.45 109 25 97 72 549.61 1.64 110 25 246 221 35.05 0.11 111 25 248 223 32.96 0.96 112 26 33 7 768.25 0.78 113 26 248 222 32.98 0.96 114 28 32 4 794.48 4.25 115 28 33 5 780.92 4.61 116 28 93 65 585.58 1.34 117 28 97 69 579.88 0.41 118 29 32 3 798.89 2.04 119 29 33 4 798.88 2.04 120 30 33 3 803.18 1.32 121 30 34 4 798.88 2.03 122 31 34 3 805.12 1.08 123 31 35 4 798.87 2.04 124 32 35 3 806.56 0.35 125 32 36 4 788.08 1.04 126 33 36 3 804.61 1.82 127 33 37 4 795.93 4.96 128 33 248 215 32.98 0.97 129 34 37 3 804.37 0.75 130 34 38 4 798.32 1.98 131 35 38 3 805.86 1.94 132 35 39 4 795.38 3.45 133 36 39 3 808.01 1.76 134 36 40 4 795.38 3.45 135 36 93 57 579.74 0.40 136 36 94 58 579.70 0.39 137 37 40 3 814.80 4.98 138 37 41 4 792.68 0.83 139 37 242 205 43.26 0.63 140 37 243 206 41.41 0.35 141 37 246 209 32.99 0.97 142 38 41 3 798.80 3.85 143 38 42 4 792.69 0.82 144 39 42 3 812.46 6.15 145 39 43 4 793.85 0.24 146 39 92 53 582.19 1.62 147 39 93 54 579.73 0.40 148 40 43 3 804.35 0.10 149 40 44 4 800.27 1.94 150 40 72 32 612.81 7.23 151 40 89 49 585.22 1.42 152 40 94 54 575.19 1.84 153 40 239 199 50.48 0.29 154 40 242 202 42.04 0.39 155 41 44 3 804.36 0.10 156 41 45 4 798.78 2.69 157 41 239 198 48.52 0.69 158 41 242 201 41.70 0.49 159 42 45 3 798.82 2.71 160 42 46 4 798.78 2.69 161 43 46 3 803.75 0.20 162 43 47 4 798.77 2.69 163 43 88 45 585.08 1.47 164 43 89 46 585.01 1.49 165 43 92 49 579.71 0.39 166 44 47 3 794.52 0.56 167 44 48 4 795.11 4.33 168 44 58 14 613.53 7.20 169 44 239 195 44.86 0.56 170 45 48 3 800.01 1.88 171 45 49 4 802.79 0.49 172 46 49 3 807.48 1.85 173 46 50 4 800.01 1.87 174 47 50 3 807.48 1.85 175 47 51 4 786.46 0.19 176 47 58 11 613.64 7.20 177 47 60 13 613.81 7.31 178 47 72 25 613.52 7.34 179 47 74 27 613.21 7.40 180 47 76 29 613.12 7.44 181 47 85 38 585.96 1.19 182 48 51 3 807.88 2.52 183 48 52 4 793.25 0.42 184 48 58 10 615.33 7.85 185 48 239 191 43.82 0.92 186 49 52 3 799.17 2.53 187 49 53 4 793.25 0.42 188 50 53 3 804.75 6.17 189 50 54 4 804.75 6.17 190 50 55 5 758.01 2.35 191 50 56 6 621.20 5.86 192 50 76 26 613.23 7.39 193 51 54 3 807.14 4.98 194 51 56 5 652.53 0.27 195 51 57 6 621.17 5.87 196 51 58 7 621.19 5.86 197 51 134 83 424.50 0.06 198 51 137 86 424.50 0.06 199 54 134 80 424.42 0.06 200 55 76 21 613.27 7.41 201 55 77 22 613.22 7.42 202 56 75 19 613.33 7.43 203 56 76 20 613.27 7.41 204 57 74 17 613.52 7.43 205 57 75 18 613.39 7.47 206 57 76 19 613.39 7.47 207 57 133 76 424.51 0.06 208 57 134 77 424.37 0.03 209 57 135 78 424.35 0.03 210 58 73 15 613.64 7.40 211 58 74 16 613.51 7.44 212 58 135 77 424.37 0.03 213 59 72 13 613.72 7.42 214 59 73 14 613.65 7.40 215 59 135 76 424.44 0.03 216 60 70 10 615.55 7.46 217 60 71 11 614.98 7.25 218 60 72 12 614.21 7.19 219 60 236 176 56.39 0.46 220 60 237 177 51.95 0.52 221 60 239 179 44.86 0.57 222 61 70 9 615.57 7.45 223 61 71 10 615.36 7.44 224 61 138 77 423.93 0.15 225 61 139 78 423.63 0.23 226 61 140 79 412.60 3.51 227 61 141 80 389.04 2.88 228 61 202 141 195.55 0.35 229 61 234 173 60.01 0.05 230 61 235 174 59.88 0.06 231 61 236 175 56.39 0.46 232 61 237 176 54.95 0.25 233 62 69 7 615.64 7.47 234 62 70 8 615.58 7.45 235 62 234 172 60.20 0.01 236 62 235 173 59.95 0.08 237 62 237 175 57.68 0.19 238 62 245 183 32.94 0.97 239 63 68 5 615.68 7.47 240 63 69 6 615.65 7.46 241 63 70 7 615.63 7.46 242 63 71 8 615.63 7.46 243 63 141 78 401.57 2.00 244 63 232 169 69.50 0.26 245 63 233 170 65.45 0.56 246 63 234 171 60.44 0.12 247 64 233 169 68.95 0.02 248 65 68 3 848.65 4.83 249 67 101 34 509.73 1.20 250 67 230 163 71.46 0.23 251 67 231 164 71.41 0.21 252 67 232 165 70.56 0.01 253 68 101 33 499.03 6.62 254 70 98 28 539.95 2.13 255 70 237 167 57.95 0.33 256 71 141 70 377.31 4.85 257 72 89 17 583.25 0.60 258 72 98 26 539.69 2.00 259 73 135 62 424.37 0.02 260 73 141 68 369.01 3.43 261 73 185 112 202.79 3.21 262 73 200 127 195.56 0.37 263 73 202 129 195.44 0.35 264 74 82 8 585.96 1.45 265 74 85 11 585.44 1.38 266 75 108 33 426.59 0.14 267 75 133 58 424.49 0.06 268 75 135 60 424.31 0.02 269 76 81 5 750.96 1.81 270 76 82 6 692.12 2.61 271 76 85 9 658.23 12.50 272 77 81 4 791.83 1.28 273 78 81 3 812.73 0.43 274 78 82 4 791.83 1.28 275 79 82 3 812.88 0.61 276 79 83 4 800.46 5.58 277 79 105 26 427.20 0.41 278 80 83 3 800.46 5.58 279 80 84 4 789.16 0.06 280 81 84 3 800.22 5.47 281 81 85 4 785.15 0.22 282 82 85 3 808.21 1.48 283 82 86 4 788.25 1.54 284 82 105 23 427.22 0.40 285 83 86 3 809.99 8.14 286 83 87 4 788.57 1.70 287 84 87 3 799.98 5.83 288 84 88 4 789.75 2.29 289 85 88 3 796.15 2.35 290 85 89 4 792.88 0.72 291 86 89 3 810.82 0.42 292 86 90 4 792.88 0.73 293 87 90 3 798.89 0.92 294 87 91 4 792.86 0.72 295 88 91 3 810.90 1.19 296 88 92 4 798.14 0.55 297 89 92 3 814.01 0.36 298 89 95 6 695.96 13.79 299 90 95 5 695.96 13.79 300 92 95 3 810.45 2.83 301 93 96 3 816.95 0.42 302 93 97 4 812.23 0.37 303 94 97 3 815.38 1.21 304 94 98 4 806.42 2.79 305 94 246 152 32.98 0.97 306 95 98 3 811.74 0.12 307 96 99 3 864.83 3.10 308 98 246 148 32.98 0.97 309 102 143 41 267.12 4.90 310 102 145 43 223.37 2.83 311 102 232 130 69.38 0.14 312 104 143 39 216.80 0.36 313 104 145 41 203.99 3.71 314 104 189 85 202.83 3.16 315 104 193 89 197.43 0.42 316 104 195 91 197.26 0.33 317 104 198 94 196.03 0.16 318 105 189 84 202.68 3.14 319 105 192 87 200.88 2.19 320 105 193 88 197.28 0.36 321 106 187 81 202.69 3.19 322 106 188 82 202.65 3.15 323 106 189 83 202.28 2.96 324 106 192 86 200.86 2.19 325 107 187 80 202.41 3.24 326 107 188 81 202.59 3.15 327 107 192 85 200.86 2.19 328 108 130 22 424.48 0.07 329 108 131 23 424.32 0.04 330 108 133 25 423.95 0.14 331 108 185 77 202.52 3.29 332 108 186 78 202.45 3.26 333 108 187 79 202.41 3.24 334 109 186 77 202.41 3.24 335 109 187 78 202.42 3.23 336 109 188 79 202.19 3.12 337 109 197 88 195.83 0.08 338 110 127 17 424.62 0.03 339 110 130 20 424.17 0.18 340 110 131 21 423.98 0.10 341 112 130 18 424.63 0.04 342 113 121 8 424.75 0.01 343 113 127 14 424.66 0.04 344 113 130 17 424.64 0.04 345 116 119 3 870.77 6.01 346 119 124 5 752.27 9.84 347 120 123 3 808.32 1.12 348 120 124 4 788.05 1.47 349 121 124 3 788.06 1.46 350 121 127 6 699.74 24.87 351 122 128 6 730.06 9.71 352 122 131 9 626.67 8.95 353 122 132 10 624.91 9.83 354 123 128 5 749.73 19.54 355 124 127 3 800.60 1.54 356 124 128 4 771.84 9.80 357 125 128 3 802.48 4.03 358 125 129 4 789.50 1.06 359 126 129 3 796.35 0.96 360 126 130 4 789.51 1.05 361 127 130 3 819.30 3.52 362 127 131 4 789.62 1.11 363 128 131 3 797.38 2.46 364 128 132 4 773.02 9.40 365 129 133 4 754.55 16.96 366 130 133 3 786.89 2.48 367 135 185 50 202.69 3.20 368 135 202 67 191.97 0.24 369 136 183 47 202.61 3.20 370 136 202 66 192.09 0.18 371 137 236 99 57.13 0.08 372 138 202 64 195.41 0.34 373 138 203 65 193.00 0.27 374 138 204 66 189.38 1.30 375 138 236 98 58.30 0.18 376 139 203 64 195.44 0.35 377 139 204 65 191.94 0.11 378 139 235 96 59.95 0.07 379 139 236 97 58.65 0.35 380 140 202 62 195.55 0.35 381 140 203 63 195.44 0.35 382 140 204 64 195.33 0.38 383 140 205 65 194.39 0.07 384 140 206 66 166.64 0.31 385 140 209 69 144.74 1.15 386 140 234 94 60.05 0.03 387 140 235 95 59.86 0.11 388 141 200 59 196.13 0.14 389 141 201 60 195.71 0.33 390 141 202 61 195.53 0.35 391 141 209 68 140.43 0.93 392 141 233 92 63.34 1.03 393 141 234 93 60.00 0.05 394 142 200 58 196.16 0.14 395 142 201 59 195.82 0.27 396 142 209 67 137.82 2.23 397 142 232 90 65.41 0.56 398 142 233 91 60.39 0.14 399 143 198 55 196.93 0.08 400 143 199 56 196.24 0.10 401 143 200 57 196.12 0.13 402 143 231 88 68.64 0.14 403 143 232 89 65.02 0.38 404 144 198 54 196.94 0.08 405 144 199 55 196.31 0.08 406 144 201 57 196.25 0.10 407 144 213 69 130.40 1.50 408 144 216 72 128.22 1.44 409 144 228 84 71.44 0.19 410 144 230 86 70.67 0.06 411 144 231 87 68.36 0.01 412 145 195 50 203.77 2.73 413 145 197 52 197.87 0.49 414 145 198 53 196.94 0.08 415 145 228 83 70.92 0.07 416 145 229 84 70.71 0.09 417 145 230 85 70.71 0.09 418 146 195 49 203.80 2.72 419 146 196 50 203.77 2.72 420 146 197 51 202.86 2.97 421 146 199 53 197.86 0.49 422 146 226 80 87.47 0.35 423 146 227 81 73.84 0.59 424 146 228 82 70.91 0.07 425 147 195 48 203.86 2.70 426 147 196 49 203.75 2.71 427 147 225 78 90.27 0.62 428 147 226 79 87.84 0.20 429 147 227 80 75.56 0.81 430 148 156 8 253.93 3.43 431 148 157 9 253.96 3.41 432 148 168 20 230.37 2.24 433 148 196 48 203.88 2.69 434 148 197 49 203.88 2.69 435 148 221 73 99.12 2.03 436 148 225 77 88.64 0.18 437 149 154 5 530.33 111.17 438 149 155 6 263.23 3.91 439 149 156 7 256.83 2.00 440 149 224 75 93.94 0.49 441 149 225 76 90.56 0.77 442 149 227 78 87.95 0.14 443 150 154 4 734.84 21.25 444 150 155 5 273.50 1.24 445 150 157 7 258.17 1.38 446 151 154 3 821.82 11.52 447 151 155 4 651.45 0.54 448 151 227 76 93.86 0.45 449 152 155 3 839.74 2.56 450 153 224 71 101.28 2.47 451 154 224 70 97.96 1.48 452 154 225 71 93.95 0.48 453 155 158 3 817.00 4.46 454 155 159 4 803.58 0.77 455 155 225 70 93.95 0.48 456 156 159 3 806.60 0.74 457 156 164 8 671.14 9.81 458 156 167 11 636.56 7.46 459 156 168 12 636.56 7.46 460 156 221 65 101.17 2.48 461 156 225 69 93.89 0.47 462 157 164 7 731.89 2.94 463 157 196 39 204.24 2.68 464 157 197 40 204.24 2.68 465 159 163 4 760.84 11.46 466 159 164 5 758.37 10.25 467 159 167 8 742.53 2.33 468 159 225 66 99.74 2.07 469 160 163 3 815.44 0.80 470 160 164 4 778.98 2.41 471 161 164 3 812.49 7.12 472 161 165 4 798.31 0.04 473 162 165 3 816.50 5.11 474 162 166 4 802.34 1.97 475 163 166 3 802.56 1.91 476 163 167 4 802.38 2.00 477 164 167 3 807.36 0.49 478 164 168 4 802.41 1.99 479 165 168 3 821.81 6.73 480 165 169 4 806.65 4.11 481 166 169 3 811.61 1.63 482 166 170 4 811.62 1.64 483 167 170 3 817.31 1.21 484 167 171 4 810.02 2.43 485 167 220 53 108.75 1.41 486 167 221 54 103.38 3.46 487 167 225 58 102.02 2.79 488 168 171 3 810.03 2.43 489 168 172 4 810.02 2.43 490 168 221 53 103.41 3.48 491 169 172 3 811.26 1.81 492 169 173 4 807.90 3.48 493 170 173 3 807.85 3.46 494 170 174 4 799.35 0.80 495 170 219 49 118.18 0.08 496 171 174 3 807.80 3.47 497 171 175 4 795.97 0.96 498 171 216 45 131.54 1.83 499 171 219 48 117.05 0.61 500 171 221 50 105.88 2.23 501 172 175 3 799.48 0.38 502 172 176 4 795.85 1.06 503 173 176 3 801.87 1.64 504 173 177 4 796.18 1.21 505 174 177 3 815.37 0.06 506 174 178 4 804.13 1.05 507 174 212 38 138.02 2.06 508 174 215 41 137.44 1.97 509 174 216 42 132.55 1.35 510 174 219 45 125.08 0.99 511 175 178 3 814.27 4.57 512 175 179 4 802.89 1.67 513 175 212 37 137.98 2.08 514 176 179 3 805.69 0.28 515 177 215 38 137.96 2.08 516 178 181 3 788.50 3.47 517 178 182 4 733.92 7.50 518 178 208 30 185.68 5.00 519 178 211 33 138.08 2.09 520 178 212 34 137.97 2.08 521 178 215 37 137.93 2.06 522 181 203 22 197.18 0.95 523 181 208 27 138.21 2.12 524 182 201 19 197.66 1.01 525 182 202 20 197.44 1.06 526 182 203 21 197.09 0.98 527 182 208 26 137.95 2.16 528 182 212 30 137.44 2.34 529 183 201 18 197.95 1.16 530 183 202 19 197.43 1.07 531 184 199 15 198.53 1.42 532 184 200 16 198.30 1.33 533 184 201 17 197.70 1.36 534 185 199 14 198.55 1.44 535 185 200 15 198.33 1.50 536 185 202 17 197.89 1.53 537 186 197 11 199.11 1.63 538 186 198 12 199.00 1.68 539 186 199 13 198.38 1.52 540 187 197 10 199.06 1.69 541 187 198 11 199.00 1.69 542 188 192 4 800.06 0.94 543 188 196 8 199.44 1.80 544 188 197 9 199.31 1.83 545 189 192 3 787.18 2.75 546 189 193 4 717.37 3.48 547 189 195 6 252.06 7.58 548 189 196 7 199.45 1.80 549 189 197 8 199.37 1.84 550 190 196 6 199.46 1.80 551 195 228 33 73.04 0.99 552 195 229 34 70.88 0.07 553 201 209 8 229.75 1.62 554 201 212 11 228.81 1.16 555 201 213 12 228.82 1.16 556 202 209 7 632.03 7.67 557 203 208 5 656.10 7.44 558 203 209 6 636.12 9.65 559 205 208 3 817.01 0.46 560 206 209 3 811.43 3.25 561 207 210 3 817.00 0.45 562 207 211 4 789.83 4.31 563 208 211 3 800.73 9.26 564 208 212 4 786.34 6.05 565 209 212 3 793.05 2.71 566 209 213 4 781.50 0.38 567 209 233 24 65.78 0.39 568 210 213 3 804.65 3.10 569 210 214 4 761.81 10.24 570 211 214 3 792.94 2.66 571 211 215 4 789.37 3.54 572 212 215 3 798.33 8.02 573 212 216 4 789.37 3.54 574 213 216 3 805.64 3.76 575 213 217 4 796.06 0.19 576 213 231 18 71.16 0.07 577 214 217 3 805.63 3.76 578 214 218 4 796.31 0.31 579 215 218 3 827.93 1.70 580 216 219 3 812.45 3.27 581 216 222 6 648.15 2.30 582 217 222 5 648.16 2.30 583 217 223 6 647.99 2.39 584 217 228 11 242.81 4.14 585 217 231 14 148.55 30.09 586 219 222 3 813.27 4.53 587 219 223 4 713.74 4.96 588 220 223 3 788.33 6.49 589 220 224 4 699.31 2.41 590 220 225 5 699.31 2.41 591 221 225 4 699.30 2.41 592 221 226 5 699.29 2.41 593 222 225 3 781.60 1.44 594 222 226 4 680.43 11.80 595 237 241 4 750.71 7.48 596 237 242 5 701.18 16.31 597 237 245 8 279.68 1.11 598 238 241 3 810.32 2.39 599 238 242 4 791.16 4.61 600 239 242 3 811.72 6.68 601 241 244 3 797.63 0.53 602 241 245 4 765.88 1.50 603 242 245 3 793.78 3.81 604 242 246 4 775.05 3.08 605 243 246 3 800.43 1.12 606 243 247 4 755.99 11.12 607 244 247 3 789.85 5.80