BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 89 87 514.96 3.10 2 3 49 46 577.41 0.30 3 3 50 47 576.75 0.51 4 3 51 48 576.70 0.53 5 3 86 83 530.32 1.46 6 4 48 44 578.75 0.33 7 4 49 45 577.51 0.35 8 4 50 46 576.75 0.51 9 4 83 79 552.74 0.82 10 4 86 82 537.81 2.19 11 5 26 21 586.49 0.33 12 5 27 22 586.38 0.31 13 5 44 39 579.55 0.14 14 5 45 40 579.30 0.23 15 5 46 41 579.19 0.27 16 5 47 42 578.97 0.24 17 5 48 43 578.75 0.33 18 5 49 44 577.87 0.47 19 5 83 78 554.99 0.21 20 6 24 18 587.67 0.59 21 6 25 19 586.62 0.29 22 6 26 20 586.48 0.33 23 6 27 21 586.41 0.29 24 6 47 41 578.98 0.23 25 6 48 42 578.88 0.28 26 6 50 44 578.75 0.33 27 7 25 18 586.86 0.18 28 7 26 19 586.49 0.32 29 7 27 20 586.48 0.33 30 7 40 33 581.32 0.43 31 7 41 34 581.29 0.45 32 7 47 40 579.18 0.26 33 8 24 16 588.88 1.02 34 8 25 17 588.76 1.06 35 9 22 13 590.64 0.60 36 9 23 14 589.00 1.04 37 9 24 15 588.88 1.02 38 9 25 16 588.88 1.02 39 10 13 3 836.52 2.16 40 10 22 12 590.64 0.60 41 10 23 13 590.36 0.50 42 10 25 15 589.13 1.11 43 11 22 11 590.65 0.60 44 11 62 51 576.95 0.55 45 12 20 8 592.70 0.21 46 12 21 9 592.66 0.20 47 12 22 10 592.27 0.37 48 12 62 50 577.11 0.50 49 13 16 3 842.03 0.60 50 13 20 7 593.51 0.56 51 13 21 8 592.68 0.21 52 13 23 10 592.54 0.24 53 14 19 5 607.18 1.52 54 14 20 6 593.58 0.53 55 15 166 151 306.69 0.40 56 16 21 5 650.63 4.84 57 16 23 7 601.38 3.38 58 16 74 58 566.86 2.44 59 16 127 111 446.22 0.55 60 16 166 150 306.49 0.35 61 17 21 4 800.64 1.14 62 17 127 110 447.81 0.24 63 17 164 147 307.42 0.29 64 17 166 149 306.88 0.39 65 18 21 3 902.77 0.30 66 18 70 52 575.72 0.14 67 18 71 53 570.57 1.94 68 18 125 107 454.70 0.14 69 18 164 146 307.50 0.28 70 19 69 50 576.25 0.40 71 19 70 51 575.21 0.37 72 19 71 52 570.53 1.96 73 20 65 45 576.63 0.42 74 20 66 46 576.50 0.37 75 20 69 49 575.68 0.11 76 20 71 51 569.37 2.35 77 20 72 52 569.37 2.35 78 21 71 50 569.04 2.19 79 21 72 51 567.29 2.39 80 21 73 52 567.00 2.44 81 21 74 53 566.93 2.39 82 21 100 79 524.66 2.58 83 21 101 80 523.53 2.44 84 21 102 81 522.63 2.21 85 22 55 33 578.21 0.63 86 22 58 36 578.00 0.71 87 22 59 37 577.01 0.59 88 22 62 40 576.88 0.53 89 22 72 50 567.22 2.35 90 22 73 51 566.76 2.32 91 22 74 52 566.70 2.29 92 22 90 68 559.02 0.12 93 23 74 51 566.61 2.29 94 23 76 53 566.36 2.29 95 24 74 50 566.57 2.26 96 24 75 51 566.37 2.29 97 24 76 52 566.29 2.30 98 24 90 66 558.90 0.16 99 25 76 51 566.19 2.27 100 25 77 52 565.63 2.15 101 26 75 49 566.26 2.28 102 26 76 50 566.19 2.27 103 26 77 51 565.82 2.18 104 26 78 52 565.71 2.16 105 26 80 54 564.20 2.01 106 26 83 57 559.08 0.17 107 26 86 60 559.08 0.17 108 26 87 61 559.04 0.15 109 27 40 13 581.91 0.71 110 27 41 14 581.83 0.73 111 27 43 16 581.78 0.73 112 27 46 19 579.31 0.24 113 27 47 20 579.17 0.26 114 27 83 56 559.09 0.16 115 28 41 13 581.87 0.71 116 28 42 14 581.82 0.72 117 28 43 15 581.75 0.71 118 28 44 16 581.13 0.92 119 28 78 50 565.79 2.13 120 28 79 51 565.75 2.15 121 28 82 54 564.91 2.25 122 29 32 3 925.80 2.68 123 29 41 12 581.90 0.70 124 29 42 13 581.82 0.72 125 29 78 49 565.88 2.16 126 29 79 50 565.75 2.15 127 29 82 53 565.66 2.18 128 29 106 77 465.67 1.11 129 30 82 52 565.71 2.17 130 30 108 78 465.28 1.26 131 30 110 80 464.95 1.19 132 31 106 75 465.75 1.05 133 31 108 77 465.31 1.27 134 31 131 100 418.87 0.86 135 31 138 107 363.70 3.62 136 31 142 111 361.92 2.89 137 31 144 113 359.89 1.98 138 31 149 118 348.29 0.03 139 32 42 10 582.11 0.71 140 32 142 110 362.89 3.38 141 33 36 3 803.65 4.16 142 33 42 9 602.45 4.17 143 33 131 98 418.84 0.83 144 33 141 108 363.83 3.67 145 33 142 109 363.13 3.55 146 33 209 176 118.06 0.49 147 34 140 106 364.04 3.71 148 34 141 107 364.01 3.71 149 34 173 139 247.41 12.37 150 34 209 175 118.06 0.49 151 34 216 182 116.00 0.48 152 34 217 183 115.92 0.47 153 34 218 184 115.12 0.85 154 35 131 96 418.98 0.81 155 35 141 106 364.03 3.71 156 35 168 133 297.15 0.07 157 35 201 166 144.97 6.73 158 35 216 181 116.83 0.55 159 35 218 183 115.12 0.85 160 36 39 3 841.62 0.36 161 36 42 6 757.85 2.59 162 36 209 173 118.03 0.49 163 37 202 165 119.19 0.78 164 37 204 167 118.06 0.49 165 37 205 168 118.00 0.51 166 37 208 171 117.97 0.48 167 38 41 3 824.13 1.23 168 38 42 4 827.17 0.29 169 38 77 39 566.11 2.31 170 38 208 170 117.67 0.45 171 39 42 3 844.07 8.08 172 39 208 169 117.46 0.41 173 39 209 170 117.47 0.42 174 39 212 173 117.43 0.40 175 39 214 175 117.20 0.51 176 40 208 168 117.41 0.43 177 40 212 172 117.36 0.42 178 41 77 36 566.10 2.28 179 41 78 37 565.76 2.31 180 42 209 167 117.47 0.42 181 50 54 4 820.62 2.51 182 50 86 36 553.77 0.26 183 51 54 3 822.00 1.82 184 51 55 4 821.22 3.46 185 51 86 35 553.89 0.25 186 52 55 3 827.78 0.18 187 52 56 4 819.30 3.18 188 52 86 34 554.75 0.09 189 52 89 37 552.34 0.96 190 52 90 38 552.32 0.95 191 52 93 41 550.62 0.65 192 53 56 3 836.35 4.46 193 53 57 4 819.12 2.32 194 54 57 3 825.00 1.56 195 54 58 4 821.06 2.40 196 55 58 3 823.66 1.09 197 55 59 4 820.16 2.83 198 55 86 31 558.45 0.14 199 55 90 35 552.98 0.94 200 56 59 3 822.82 1.51 201 56 60 4 819.40 3.21 202 56 90 34 553.38 1.12 203 56 93 37 551.12 0.89 204 56 94 38 550.62 0.65 205 56 97 41 545.48 0.08 206 57 61 4 839.37 3.52 207 58 61 3 839.37 3.52 208 58 62 4 832.85 0.26 209 59 62 3 832.85 0.26 210 59 63 4 832.84 0.25 211 59 66 7 780.02 2.01 212 59 73 14 566.70 2.29 213 59 90 31 556.57 0.26 214 59 99 40 532.58 1.12 215 60 63 3 832.83 0.25 216 62 65 3 824.25 1.09 217 62 66 4 804.39 6.34 218 63 66 3 817.38 0.15 219 63 67 4 810.87 3.10 220 63 97 34 550.22 0.49 221 63 99 36 544.22 0.32 222 64 67 3 836.34 4.96 223 64 68 4 810.87 3.10 224 65 68 3 820.25 1.29 225 65 69 4 810.85 3.09 226 66 69 3 817.32 0.17 227 66 70 4 789.46 7.61 228 66 71 5 698.98 13.02 229 66 72 6 640.75 21.80 230 66 73 7 660.91 11.72 231 66 99 33 543.37 0.90 232 67 70 3 831.96 7.15 233 71 100 29 524.68 2.58 234 72 99 27 526.50 1.68 235 72 100 28 523.64 2.47 236 73 90 17 559.02 0.12 237 73 91 18 558.93 0.12 238 73 94 21 551.49 1.11 239 73 99 26 527.10 1.38 240 73 100 27 523.50 2.46 241 73 101 28 516.78 3.56 242 73 102 29 514.52 4.41 243 74 90 16 559.02 0.12 244 74 102 28 500.24 3.71 245 74 104 30 490.51 7.64 246 74 127 53 439.06 2.95 247 75 80 5 604.69 5.12 248 75 87 12 559.11 0.14 249 75 90 15 558.97 0.15 250 75 91 16 558.94 0.17 251 75 102 27 499.76 3.47 252 75 103 28 469.79 2.58 253 75 104 29 467.78 1.66 254 76 80 4 631.07 9.94 255 76 104 28 466.06 1.04 256 79 82 3 831.07 3.43 257 79 105 26 465.67 1.11 258 79 106 27 465.31 1.27 259 80 83 3 820.86 5.63 260 80 84 4 797.11 2.98 261 80 87 7 715.40 9.37 262 80 103 23 469.67 2.60 263 81 84 3 804.49 2.02 264 81 105 24 465.31 1.27 265 81 109 28 456.38 0.29 266 81 119 38 456.01 0.29 267 81 152 71 347.75 0.11 268 82 110 28 456.37 0.29 269 83 86 3 810.20 5.50 270 83 87 4 795.38 3.51 271 84 87 3 825.23 12.94 272 84 88 4 800.88 0.76 273 84 115 31 456.11 0.34 274 84 116 32 456.05 0.30 275 84 119 35 455.99 0.29 276 85 88 3 848.10 1.50 277 85 89 4 804.28 0.95 278 85 115 30 456.11 0.34 279 86 89 3 821.76 1.30 280 86 90 4 803.44 1.37 281 87 90 3 822.77 2.96 282 87 91 4 808.91 3.98 283 87 119 32 456.00 0.29 284 88 91 3 826.34 2.31 285 88 92 4 797.49 1.61 286 88 115 27 456.24 0.29 287 88 118 30 456.19 0.29 288 88 119 31 455.99 0.29 289 88 122 34 455.01 0.24 290 89 92 3 821.51 0.12 291 89 93 4 811.33 4.88 292 90 93 3 826.03 2.46 293 90 94 4 811.34 4.88 294 91 94 3 807.78 3.45 295 91 95 4 807.91 3.39 296 91 101 10 508.84 0.33 297 91 102 11 511.01 1.41 298 91 103 12 505.50 1.82 299 91 119 28 456.17 0.29 300 91 122 31 455.01 0.24 301 91 123 32 455.01 0.24 302 92 95 3 823.50 1.46 303 92 96 4 809.01 3.93 304 92 118 26 456.23 0.29 305 92 122 30 455.02 0.24 306 93 96 3 823.97 3.55 307 93 97 4 805.94 2.40 308 94 97 3 810.60 2.58 309 94 98 4 799.70 0.73 310 94 99 5 736.90 10.21 311 94 101 7 638.89 11.45 312 95 98 3 843.70 3.15 313 95 99 4 779.07 11.01 314 95 101 6 736.90 10.21 315 95 122 27 455.39 0.37 316 95 124 29 455.03 0.24 317 100 124 24 454.94 0.20 318 100 125 25 454.85 0.16 319 101 122 21 455.05 0.23 320 101 123 22 455.00 0.24 321 101 124 23 454.80 0.15 322 101 125 24 454.64 0.10 323 102 125 23 450.94 1.39 324 102 127 25 442.21 1.38 325 103 119 16 456.16 0.31 326 103 123 20 454.80 0.15 327 103 125 22 453.10 0.34 328 103 126 23 430.97 0.27 329 103 127 24 430.88 0.30 330 104 127 23 425.00 1.54 331 104 166 62 303.89 0.32 332 105 127 22 425.36 1.71 333 105 128 23 421.57 1.16 334 105 129 24 419.52 0.70 335 105 152 47 347.82 0.11 336 106 128 22 421.67 1.20 337 106 129 23 419.08 0.76 338 106 131 25 418.87 0.85 339 106 149 43 348.16 0.09 340 107 128 21 421.68 1.21 341 107 148 41 348.38 0.07 342 107 149 42 348.16 0.09 343 107 152 45 347.78 0.13 344 108 144 36 359.95 1.96 345 108 148 40 348.28 0.04 346 109 112 3 873.93 0.06 347 109 116 7 690.93 2.27 348 109 148 39 348.25 0.04 349 109 152 43 347.79 0.13 350 110 148 38 348.26 0.04 351 111 116 5 791.87 2.10 352 112 116 4 791.87 2.10 353 112 148 36 348.28 0.04 354 112 151 39 348.06 0.02 355 112 152 40 347.75 0.12 356 112 155 43 341.53 1.17 357 113 116 3 825.84 0.70 358 114 117 3 827.12 1.34 359 114 118 4 810.74 2.04 360 115 118 3 815.83 0.48 361 115 119 4 801.47 2.59 362 116 119 3 832.28 4.66 363 116 120 4 811.70 1.57 364 116 152 36 347.73 0.12 365 116 155 39 337.22 0.98 366 117 120 3 829.02 2.12 367 117 121 4 802.41 3.08 368 117 155 38 336.34 1.43 369 117 159 42 311.35 1.40 370 118 121 3 824.06 4.60 371 118 122 4 811.71 1.57 372 119 122 3 827.08 3.10 373 119 123 4 807.51 0.39 374 120 123 3 807.51 0.39 375 120 126 6 725.01 20.95 376 120 152 32 347.73 0.12 377 120 155 35 336.32 1.43 378 120 156 36 332.60 0.40 379 120 159 39 311.34 1.40 380 120 161 41 307.66 0.28 381 123 126 3 883.05 2.05 382 124 162 38 307.59 0.30 383 125 162 37 307.57 0.31 384 125 163 38 307.55 0.30 385 125 164 39 307.50 0.28 386 126 156 30 332.58 0.39 387 126 161 35 307.75 0.27 388 126 162 36 307.59 0.30 389 126 163 37 307.44 0.29 390 126 164 38 307.39 0.28 391 127 164 37 306.75 0.45 392 127 166 39 304.17 0.18 393 128 149 21 348.37 0.07 394 128 152 24 347.88 0.14 395 128 153 25 347.75 0.12 396 128 156 28 334.89 0.73 397 128 163 35 307.44 0.28 398 128 164 36 306.81 0.48 399 128 165 37 304.67 0.43 400 128 166 38 303.89 0.32 401 129 149 20 348.38 0.07 402 129 166 37 303.67 0.27 403 130 133 3 883.91 1.64 404 130 149 19 348.39 0.07 405 130 150 20 348.36 0.07 406 130 165 35 306.42 0.53 407 130 166 36 305.16 0.95 408 130 167 37 303.53 0.20 409 130 183 53 204.17 1.56 410 130 186 56 204.13 1.54 411 130 187 57 204.11 1.54 412 131 138 7 494.70 61.58 413 131 141 10 364.07 3.73 414 131 142 11 364.05 3.72 415 131 167 36 303.41 0.17 416 132 137 5 689.72 0.07 417 132 138 6 494.70 61.58 418 132 141 9 364.16 3.71 419 132 146 14 363.63 3.75 420 132 173 41 248.64 11.76 421 132 174 42 247.34 12.35 422 132 179 47 204.27 1.59 423 133 146 13 363.81 3.66 424 133 150 17 348.82 0.21 425 133 182 49 204.24 1.58 426 133 183 50 204.19 1.56 427 133 186 53 204.18 1.56 428 134 137 3 832.85 6.15 429 134 138 4 787.25 10.71 430 134 146 12 363.87 3.68 431 134 182 48 204.30 1.59 432 135 138 3 787.26 10.71 433 135 139 4 787.26 10.71 434 135 145 10 363.87 3.68 435 135 146 11 363.87 3.68 436 135 147 12 363.87 3.68 437 137 140 3 824.02 4.45 438 137 141 4 788.84 4.73 439 137 173 36 248.65 11.75 440 138 141 3 812.75 1.12 441 138 142 4 784.28 2.46 442 138 143 5 753.99 12.67 443 138 144 6 653.68 3.95 444 138 145 7 624.08 10.00 445 138 146 8 475.50 59.50 446 139 143 4 697.01 15.81 447 139 144 5 646.79 7.38 448 139 145 6 633.17 14.19 449 140 173 33 248.65 11.75 450 141 170 29 284.25 1.87 451 141 173 32 247.36 12.35 452 146 149 3 826.69 3.23 453 146 150 4 805.41 1.76 454 147 150 3 809.26 0.16 455 147 151 4 802.14 3.39 456 148 151 3 817.00 0.77 457 148 152 4 802.17 3.38 458 149 152 3 810.51 0.66 459 149 153 4 807.54 0.70 460 150 153 3 810.66 2.25 461 150 154 4 807.35 0.60 462 150 186 36 204.06 1.55 463 151 154 3 818.05 1.44 464 151 155 4 807.36 0.59 465 152 155 3 807.46 0.64 466 152 156 4 807.38 0.59 467 153 156 3 807.59 0.49 468 153 157 4 807.55 0.51 469 153 186 33 204.07 1.55 470 153 190 37 199.78 0.36 471 154 157 3 815.00 3.23 472 154 158 4 807.01 0.22 473 154 186 32 204.06 1.55 474 155 158 3 814.45 3.50 475 155 159 4 807.01 0.21 476 156 159 3 814.45 3.50 477 156 160 4 807.01 0.21 478 156 161 5 761.44 10.51 479 156 163 7 668.69 10.18 480 156 192 36 199.58 0.41 481 157 160 3 810.42 1.52 482 157 190 33 199.78 0.36 483 157 192 35 199.58 0.41 484 160 192 32 199.57 0.41 485 161 192 31 199.57 0.41 486 162 192 30 199.58 0.41 487 163 192 29 199.58 0.42 488 163 193 30 199.16 0.38 489 163 194 31 198.85 0.24 490 163 195 32 198.73 0.29 491 164 194 30 198.86 0.24 492 164 195 31 197.12 0.48 493 164 197 33 185.75 0.78 494 165 183 18 204.17 1.56 495 165 187 22 203.94 1.46 496 165 193 28 199.52 0.45 497 165 195 30 198.52 0.07 498 165 196 31 187.66 1.73 499 165 197 32 185.77 0.79 500 166 197 31 184.48 1.42 501 166 199 33 184.10 1.40 502 167 174 7 618.37 8.13 503 167 179 12 204.37 1.55 504 167 183 16 204.19 1.56 505 167 197 30 186.40 2.36 506 167 198 31 184.43 1.42 507 167 199 32 184.10 1.40 508 167 232 65 65.33 1.99 509 168 174 6 618.37 8.13 510 168 199 31 184.09 1.41 511 168 201 33 184.01 1.38 512 169 174 5 618.37 8.13 513 169 175 6 618.34 8.14 514 169 198 29 184.53 1.42 515 169 199 30 184.44 1.43 516 169 200 31 184.19 1.42 517 169 201 32 184.08 1.41 518 169 224 55 68.86 0.64 519 169 229 60 66.20 1.56 520 170 173 3 832.56 1.79 521 170 174 4 773.41 1.06 522 170 175 5 694.70 17.20 523 170 201 31 183.95 1.43 524 170 218 48 115.91 0.47 525 170 221 51 111.67 0.20 526 171 175 4 773.43 1.06 527 171 201 30 184.07 1.36 528 171 221 50 111.69 0.20 529 171 222 51 111.66 0.20 530 171 224 53 69.81 0.18 531 172 175 3 837.20 0.54 532 175 228 53 68.07 0.67 533 175 232 57 65.63 1.84 534 176 179 3 842.17 3.06 535 176 180 4 811.54 3.04 536 177 180 3 819.40 0.90 537 177 181 4 811.55 3.03 538 177 231 54 66.25 1.57 539 177 235 58 65.29 1.96 540 178 181 3 827.78 2.86 541 178 182 4 812.29 2.66 542 179 182 3 832.74 1.66 543 179 183 4 806.10 0.30 544 180 183 3 818.26 5.24 545 180 184 4 804.03 0.72 546 180 232 52 65.34 1.99 547 180 235 55 64.52 1.58 548 180 236 56 64.29 1.64 549 181 184 3 809.00 3.79 550 181 185 4 797.39 2.59 551 181 235 54 64.25 1.59 552 181 238 57 64.00 1.72 553 181 239 58 63.50 1.56 554 182 185 3 812.44 2.71 555 182 186 4 800.39 0.51 556 183 186 3 812.25 2.19 557 183 187 4 815.09 1.52 558 183 196 13 198.87 0.23 559 183 236 53 64.00 1.72 560 184 187 3 836.25 1.29 561 184 188 4 820.78 2.70 562 184 236 52 64.00 1.72 563 184 239 55 48.85 0.91 564 184 241 57 40.62 0.53 565 185 188 3 833.56 2.64 566 185 189 4 823.09 3.28 567 185 239 54 51.61 0.83 568 186 189 3 846.77 6.08 569 186 190 4 837.73 10.60 570 187 190 3 831.46 1.22 571 187 193 6 629.43 9.13 572 187 196 9 199.50 0.43 573 187 241 54 40.36 0.66 574 188 238 50 63.88 1.75 575 188 239 51 42.86 0.11 576 188 241 53 40.58 0.77 577 190 193 3 855.56 3.37 578 193 196 3 920.15 1.69 579 193 241 48 36.54 0.54 580 194 242 48 36.06 0.71 581 195 242 47 36.03 0.72 582 195 244 49 31.57 1.23 583 196 236 40 64.00 1.71 584 196 241 45 36.55 0.54 585 196 243 47 33.33 0.35 586 196 244 48 31.51 1.26 587 197 244 47 29.70 1.54 588 198 229 31 65.57 1.88 589 198 232 34 65.33 1.97 590 198 233 35 64.89 1.78 591 198 236 38 64.31 1.62 592 198 243 45 36.08 0.70 593 198 244 46 31.11 0.90 594 198 245 47 17.99 0.22 595 199 229 30 65.61 1.85 596 200 224 24 68.84 0.65 597 200 229 29 66.20 1.56 598 201 216 15 117.25 0.41 599 201 218 17 115.89 0.46 600 201 221 20 111.66 0.19 601 201 223 22 68.86 0.65 602 201 224 23 68.86 0.64 603 202 223 21 68.86 0.65 604 203 206 3 857.83 0.57 605 203 216 13 117.12 0.45 606 203 219 16 114.83 0.97 607 205 208 3 813.78 2.06 608 205 209 4 814.81 1.54 609 206 209 3 819.65 0.30 610 206 210 4 802.70 3.30 611 206 216 10 116.53 0.22 612 207 210 3 822.85 6.78 613 207 211 4 789.84 8.94 614 208 211 3 823.80 6.30 615 208 212 4 802.07 3.42 616 209 212 3 807.05 2.07 617 209 213 4 801.91 3.34 618 209 214 5 751.34 9.20 619 209 215 6 751.32 9.19 620 209 216 7 370.68 126.39 621 210 213 3 802.61 2.98 622 210 214 4 794.54 5.38 623 216 219 3 825.17 7.54 624 218 221 3 797.35 1.41 625 224 228 4 790.93 5.98 626 224 229 5 786.22 8.34 627 225 228 3 814.62 1.95 628 225 229 4 800.20 1.81 629 226 229 3 807.26 1.73 630 226 230 4 788.85 7.49 631 227 230 3 807.39 0.58 632 227 231 4 802.90 0.63 633 228 231 3 813.39 0.49 634 228 232 4 809.34 1.54 635 229 232 3 809.35 1.53 636 229 233 4 809.33 1.53 637 229 245 16 33.97 0.04 638 230 233 3 814.65 4.19 639 230 234 4 809.34 1.53 640 230 245 15 36.50 0.52 641 231 234 3 830.77 3.73 642 231 235 4 809.32 1.53 643 232 235 3 820.58 3.44 644 232 236 4 819.90 3.77 645 233 236 3 823.83 1.81 646 233 237 4 808.39 1.11 647 233 243 10 36.56 0.53 648 233 244 11 36.55 0.54 649 233 245 12 36.49 0.52 650 234 237 3 808.66 1.25 651 234 238 4 808.39 1.11 652 235 238 3 832.30 2.70 653 235 239 4 832.46 2.62 654 236 239 3 832.43 2.60 655 236 243 7 36.61 0.56 656 237 240 3 815.65 5.60 657 237 241 4 796.82 5.58 658 237 243 6 36.91 0.65