BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 14 74 60 617.59 1.06 2 14 75 61 619.31 0.20 3 14 76 62 617.74 0.98 4 16 44 28 750.74 1.01 5 16 45 29 750.74 1.01 6 16 46 30 750.62 0.96 7 16 47 31 750.57 0.97 8 16 48 32 750.38 0.90 9 16 75 59 652.92 0.54 10 16 76 60 653.00 0.56 11 17 46 29 750.66 0.97 12 17 76 59 653.00 0.56 13 17 77 60 653.00 0.56 14 17 92 75 393.35 22.05 15 17 96 79 392.98 22.01 16 18 42 24 750.80 1.02 17 18 46 28 750.78 1.01 18 18 47 29 750.64 0.98 19 18 48 30 750.64 0.97 20 18 75 57 653.32 0.58 21 18 77 59 653.00 0.56 22 18 79 61 653.00 0.56 23 18 92 74 393.36 22.04 24 18 95 77 393.09 22.00 25 18 96 78 393.04 22.00 26 18 99 81 392.90 21.98 27 19 48 29 750.65 0.97 28 19 50 31 750.62 0.97 29 19 73 54 653.41 0.59 30 19 74 55 653.45 0.59 31 19 75 56 653.38 0.58 32 19 77 58 653.33 0.58 33 19 78 59 653.05 0.55 34 19 79 60 653.04 0.55 35 20 37 17 750.93 1.01 36 20 42 22 750.94 1.02 37 20 47 27 750.81 1.02 38 20 48 28 750.76 1.01 39 20 49 29 750.65 0.96 40 20 50 30 750.63 0.97 41 20 79 59 653.09 0.54 42 20 81 61 653.02 0.56 43 21 50 29 750.63 0.97 44 21 65 44 654.03 0.56 45 21 69 48 653.75 0.57 46 21 73 52 653.63 0.56 47 21 78 57 653.38 0.58 48 21 79 58 653.32 0.57 49 21 80 59 653.05 0.54 50 21 81 60 653.03 0.55 51 22 30 8 751.00 1.11 52 22 33 11 751.07 1.06 53 22 50 28 750.78 1.02 54 22 51 29 750.63 0.97 55 22 52 30 750.63 0.97 56 22 65 43 654.07 0.55 57 22 81 59 653.06 0.55 58 22 83 61 652.78 0.52 59 23 52 29 750.64 0.97 60 23 54 31 750.59 0.98 61 23 60 37 655.44 0.72 62 23 62 39 654.33 0.53 63 23 65 42 654.08 0.55 64 23 81 58 653.29 0.59 65 23 83 60 652.98 0.57 66 23 175 152 288.18 1.40 67 24 30 6 751.74 0.84 68 24 51 27 750.82 1.02 69 24 52 28 750.76 1.02 70 24 54 30 750.59 0.98 71 24 55 31 750.58 0.98 72 24 83 59 653.01 0.56 73 25 54 29 750.62 0.97 74 25 55 30 750.54 0.98 75 25 83 58 653.26 0.61 76 26 29 3 824.14 0.24 77 26 30 4 791.57 6.44 78 26 83 57 653.25 0.58 79 27 30 3 814.78 7.29 80 27 31 4 797.44 3.50 81 27 55 28 750.63 0.97 82 28 31 3 817.75 5.80 83 28 32 4 803.76 0.34 84 29 32 3 808.50 7.08 85 29 33 4 797.44 3.50 86 29 83 54 653.00 0.52 87 29 84 55 652.63 0.42 88 30 33 3 812.60 5.04 89 30 34 4 799.27 2.50 90 30 49 19 751.15 0.96 91 30 50 20 751.08 0.97 92 30 51 21 750.94 0.97 93 30 83 53 652.37 0.36 94 31 34 3 803.54 0.51 95 31 35 4 799.27 2.50 96 32 35 3 816.66 2.08 97 32 36 4 797.10 1.56 98 32 84 52 652.13 0.21 99 32 86 54 628.90 7.70 100 32 203 171 209.15 0.48 101 33 36 3 811.44 4.70 102 33 37 4 799.26 2.65 103 33 81 48 653.19 0.52 104 33 82 49 653.12 0.51 105 33 83 50 652.00 0.26 106 33 85 52 633.58 5.36 107 34 37 3 816.59 5.12 108 34 38 4 812.74 4.08 109 34 49 15 751.13 0.94 110 35 38 3 825.83 0.49 111 35 39 4 809.31 5.79 112 36 39 3 812.70 4.11 113 36 40 4 809.31 5.79 114 36 86 50 581.11 0.20 115 36 87 51 493.60 2.71 116 37 40 3 812.13 4.38 117 37 41 4 811.50 4.69 118 37 42 5 769.98 2.43 119 37 49 12 751.19 0.93 120 37 85 48 609.93 1.79 121 37 87 50 485.69 5.49 122 37 157 120 337.75 0.06 123 38 41 3 813.33 10.39 124 38 42 4 770.00 2.42 125 38 43 5 769.99 2.43 126 40 87 47 485.67 5.49 127 40 88 48 485.66 5.49 128 40 89 49 483.59 6.46 129 40 91 51 442.71 2.39 130 40 92 52 442.69 2.40 131 41 46 5 753.15 1.35 132 41 92 51 425.11 6.33 133 42 47 5 751.83 1.04 134 42 49 7 751.83 1.04 135 42 79 37 653.21 0.53 136 42 87 45 492.81 2.31 137 42 91 49 425.21 6.34 138 42 92 50 424.83 6.32 139 42 95 53 425.00 6.29 140 43 46 3 805.28 9.40 141 43 47 4 767.40 7.94 142 44 47 3 795.89 1.74 143 46 92 46 393.35 22.05 144 48 73 25 653.52 0.54 145 48 74 26 653.44 0.59 146 48 75 27 653.39 0.58 147 50 69 19 653.74 0.57 148 50 72 22 653.63 0.56 149 51 69 18 653.83 0.59 150 52 60 8 655.58 0.64 151 52 65 13 654.05 0.56 152 52 69 17 653.84 0.60 153 53 59 6 655.66 0.62 154 53 60 7 655.65 0.63 155 54 57 3 788.41 4.20 156 54 58 4 655.67 0.62 157 54 59 5 655.67 0.62 158 54 60 6 655.65 0.62 159 54 175 121 288.79 1.69 160 58 175 117 288.85 1.70 161 60 64 4 793.67 4.46 162 60 65 5 719.23 32.76 163 60 68 8 654.98 0.22 164 60 175 115 288.81 1.68 165 61 64 3 816.92 1.02 166 61 65 4 792.61 3.93 167 61 172 111 318.72 2.53 168 61 173 112 289.34 1.45 169 61 174 113 288.81 1.68 170 61 175 114 288.80 1.68 171 62 65 3 825.56 1.56 172 62 174 112 288.81 1.68 173 62 175 113 288.73 1.67 174 62 178 116 277.72 0.96 175 63 67 4 834.48 0.99 176 63 168 105 330.66 0.32 177 63 169 106 330.64 0.33 178 63 172 109 303.59 3.52 179 63 173 110 289.88 1.26 180 63 174 111 288.82 1.68 181 63 176 113 288.74 1.67 182 63 177 114 285.98 1.10 183 63 178 115 277.73 0.96 184 63 181 118 276.75 0.91 185 63 215 152 158.93 0.24 186 64 67 3 833.33 0.42 187 65 68 3 832.04 0.96 188 65 69 4 802.95 2.50 189 65 80 15 653.55 0.58 190 65 81 16 653.55 0.58 191 65 178 113 277.73 0.96 192 66 69 3 806.14 0.91 193 66 70 4 806.43 0.79 194 66 80 14 653.55 0.58 195 66 160 94 335.55 0.20 196 66 168 102 330.64 0.32 197 66 182 116 276.45 0.90 198 67 71 4 809.09 1.90 199 67 167 100 330.83 0.39 200 67 168 101 330.81 0.38 201 67 169 102 330.65 0.33 202 67 170 103 330.52 0.36 203 68 71 3 839.69 3.04 204 68 72 4 816.62 1.02 205 69 72 3 820.25 0.79 206 69 73 4 806.46 0.82 207 69 80 11 653.53 0.58 208 70 73 3 811.75 3.46 209 70 78 8 653.61 0.60 210 70 80 10 653.54 0.59 211 70 160 90 333.58 0.52 212 70 165 95 330.89 0.39 213 70 167 97 330.78 0.39 214 73 78 5 653.60 0.62 215 73 162 89 330.91 0.39 216 74 162 88 330.91 0.39 217 75 162 87 330.90 0.39 218 76 161 85 330.92 0.39 219 76 162 86 330.87 0.38 220 77 99 22 392.86 21.95 221 77 109 32 356.85 10.31 222 77 159 82 332.00 0.14 223 77 160 83 331.19 0.48 224 77 161 84 330.91 0.39 225 78 159 81 332.06 0.12 226 78 160 82 331.59 0.29 227 78 162 84 330.92 0.39 228 79 95 16 393.22 22.05 229 79 157 78 335.84 0.06 230 79 158 79 335.23 0.23 231 79 159 80 332.50 0.32 232 80 157 77 335.85 0.06 233 80 158 78 335.55 0.20 234 80 160 80 334.36 0.40 235 81 85 4 803.51 2.46 236 81 156 75 337.07 0.38 237 81 157 76 335.85 0.06 238 82 85 3 826.31 1.76 239 82 155 73 337.37 0.30 240 82 156 74 337.11 0.39 241 82 178 96 277.73 0.96 242 82 202 120 208.25 0.78 243 84 202 118 207.80 1.00 244 84 203 119 207.43 0.94 245 84 204 120 205.26 0.02 246 85 156 71 337.75 0.08 247 85 200 115 213.47 0.75 248 85 201 116 210.68 0.51 249 85 202 117 207.75 1.02 250 86 200 114 213.12 0.82 251 86 201 115 210.08 0.61 252 86 203 117 209.46 0.32 253 87 90 3 845.74 2.38 254 87 91 4 779.14 6.32 255 87 198 111 214.21 0.71 256 87 200 113 211.39 0.04 257 88 199 111 214.16 0.74 258 88 200 112 213.51 0.77 259 88 201 113 212.54 0.54 260 89 199 110 214.16 0.74 261 90 94 4 790.37 1.63 262 90 198 108 214.15 0.74 263 90 199 109 214.06 0.75 264 91 94 3 812.54 2.78 265 91 95 4 794.32 0.58 266 91 198 107 214.16 0.74 267 92 95 3 812.54 2.79 268 92 96 4 798.91 2.87 269 93 96 3 805.25 0.62 270 93 97 4 799.76 2.12 271 94 97 3 815.49 4.50 272 94 98 4 799.80 2.13 273 94 198 104 214.13 0.76 274 95 98 3 810.38 3.17 275 95 99 4 798.55 1.51 276 95 157 62 337.77 0.06 277 95 159 64 336.94 0.18 278 95 196 101 215.56 0.09 279 95 198 103 214.06 0.77 280 96 99 3 806.47 0.66 281 96 100 4 798.54 1.51 282 97 100 3 806.48 0.66 283 97 101 4 796.62 5.58 284 98 101 3 811.56 0.31 285 98 102 4 795.37 0.10 286 98 196 98 215.59 0.07 287 99 102 3 808.44 1.87 288 99 103 4 789.87 2.64 289 99 109 10 475.64 0.68 290 99 159 60 337.48 0.07 291 100 103 3 805.82 0.95 292 100 104 4 804.45 5.99 293 101 104 3 810.57 2.93 294 101 105 4 804.46 5.99 295 102 105 3 804.46 5.99 296 102 106 4 804.45 5.99 297 102 109 7 486.47 4.25 298 102 112 10 407.49 4.00 299 102 194 92 233.83 0.34 300 102 196 94 220.75 0.27 301 103 106 3 810.61 2.91 302 103 107 4 678.90 49.12 303 103 109 6 507.40 6.21 304 103 161 58 337.64 0.14 305 106 109 3 821.88 3.12 306 106 192 86 253.06 2.05 307 106 194 88 246.07 1.39 308 107 161 54 338.00 0.10 309 108 191 83 250.81 0.93 310 108 192 84 246.18 1.39 311 109 112 3 898.77 4.83 312 109 159 50 338.50 0.03 313 109 160 51 338.00 0.09 314 109 161 52 338.00 0.09 315 109 192 83 244.87 0.76 316 109 194 85 244.15 0.56 317 110 160 50 338.00 0.09 318 110 161 51 337.99 0.09 319 110 164 54 330.82 0.36 320 110 166 56 330.48 0.38 321 110 186 76 264.13 0.21 322 110 189 79 263.18 0.62 323 110 191 81 246.18 1.37 324 110 192 82 244.86 0.76 325 111 153 42 338.93 0.07 326 111 158 47 338.66 0.04 327 111 159 48 338.44 0.02 328 111 166 55 330.54 0.38 329 111 182 71 276.35 0.91 330 111 186 75 264.48 0.31 331 111 192 81 245.52 1.06 332 111 193 82 238.73 2.15 333 111 194 83 235.38 0.60 334 112 153 41 338.96 0.06 335 112 158 46 338.69 0.04 336 112 159 47 338.49 0.04 337 112 194 82 231.02 1.12 338 112 196 84 220.32 0.52 339 113 153 40 338.98 0.07 340 113 157 44 338.80 0.04 341 113 158 45 338.60 0.01 342 113 194 81 231.96 1.43 343 113 195 82 221.27 0.23 344 113 196 83 221.12 0.15 345 114 131 17 362.39 7.94 346 114 132 18 362.38 7.94 347 114 133 19 362.38 7.94 348 114 156 42 338.95 0.04 349 114 157 43 338.88 0.03 350 114 196 82 221.28 0.23 351 114 198 84 214.38 0.70 352 114 200 86 214.26 0.69 353 115 130 15 362.49 7.99 354 115 131 16 362.48 7.98 355 115 153 38 339.21 0.03 356 115 154 39 339.14 0.01 357 115 195 80 235.39 2.92 358 116 129 13 362.57 7.99 359 116 130 14 362.57 7.98 360 116 150 34 361.55 7.73 361 116 152 36 349.03 4.65 362 116 153 37 339.22 0.03 363 116 154 38 339.20 0.02 364 116 235 119 88.29 0.40 365 117 128 11 362.59 7.98 366 117 129 12 362.57 7.99 367 117 150 33 361.66 7.74 368 117 151 34 361.47 7.69 369 117 152 35 356.68 8.35 370 117 233 116 89.80 0.82 371 117 234 117 88.81 0.57 372 117 235 118 88.41 0.37 373 118 121 3 833.46 1.87 374 118 128 10 362.65 7.99 375 118 129 11 362.65 7.98 376 118 130 12 362.62 7.97 377 118 149 31 362.10 7.84 378 118 150 32 361.93 7.87 379 118 151 33 362.00 7.81 380 119 149 30 362.11 7.83 381 119 150 31 362.08 7.84 382 119 151 32 362.00 7.81 383 119 187 68 276.38 0.80 384 120 151 31 362.12 7.83 385 120 230 110 100.39 3.40 386 121 124 3 852.81 1.48 387 121 128 7 362.74 8.00 388 121 151 30 362.18 7.82 389 121 233 112 91.44 0.45 390 123 126 3 858.68 2.04 391 123 233 110 98.74 2.58 392 124 231 107 98.81 2.62 393 124 233 109 98.67 2.56 394 124 238 114 89.66 0.77 395 125 238 113 89.75 0.76 396 126 233 107 98.62 2.58 397 126 238 112 89.28 0.58 398 127 233 106 90.36 1.00 399 127 234 107 90.08 0.86 400 127 237 110 88.59 0.46 401 127 238 111 88.28 0.39 402 128 233 105 90.07 0.86 403 129 143 14 368.94 8.03 404 129 144 15 368.87 8.01 405 129 235 106 88.58 0.46 406 130 143 13 368.95 8.04 407 130 144 14 368.86 8.00 408 130 146 16 368.84 8.00 409 130 148 18 368.61 8.10 410 130 195 65 264.67 0.38 411 131 141 10 378.41 9.21 412 131 142 11 376.10 10.10 413 131 143 12 369.04 8.04 414 131 146 15 368.85 8.00 415 132 140 8 396.92 0.10 416 132 141 9 378.00 9.41 417 132 142 10 376.10 10.10 418 132 146 14 368.85 8.00 419 132 197 65 264.64 0.39 420 132 199 67 264.32 0.49 421 133 140 7 407.29 5.28 422 133 141 8 395.66 0.71 423 133 156 23 338.91 0.05 424 133 199 66 264.34 0.48 425 133 200 67 264.32 0.48 426 133 202 69 264.20 0.51 427 134 138 4 532.32 2.27 428 134 139 5 412.53 5.50 429 134 140 6 410.12 6.70 430 134 199 65 264.65 0.38 431 134 200 66 264.48 0.41 432 134 201 67 264.22 0.50 433 134 202 68 264.22 0.50 434 135 138 3 823.23 0.90 435 135 141 6 480.04 2.73 436 135 201 66 264.32 0.48 437 135 202 67 264.19 0.48 438 136 203 67 264.19 0.48 439 136 205 69 260.92 0.24 440 137 141 4 819.88 0.20 441 138 141 3 835.56 3.81 442 140 199 59 264.63 0.39 443 142 146 4 834.44 0.95 444 142 197 55 264.73 0.36 445 145 148 3 897.14 6.21 446 146 195 49 264.29 0.49 447 146 196 50 263.74 0.71 448 146 197 51 263.17 0.67 449 147 195 48 264.01 0.58 450 147 196 49 262.91 0.55 451 147 197 50 262.55 0.36 452 148 194 46 264.13 0.52 453 148 195 47 263.66 0.69 454 149 192 43 264.25 0.51 455 149 193 44 264.13 0.52 456 149 194 45 263.17 0.67 457 150 153 3 866.47 0.60 458 150 193 43 263.64 0.70 459 150 194 44 263.16 0.67 460 150 195 45 262.29 0.29 461 151 183 32 276.75 0.91 462 151 230 79 98.74 2.58 463 151 232 81 90.03 0.88 464 151 233 82 90.00 0.88 465 152 180 28 277.05 0.93 466 152 183 31 276.58 0.86 467 152 233 81 89.97 0.89 468 152 234 82 88.45 0.35 469 153 158 5 482.75 2.79 470 153 179 26 277.56 0.88 471 153 183 30 276.57 0.86 472 153 193 40 257.10 2.62 473 153 194 41 244.87 0.74 474 153 195 42 235.12 3.05 475 154 235 81 88.28 0.40 476 155 158 3 850.56 1.48 477 155 177 22 277.91 1.03 478 155 178 23 277.73 0.96 479 155 179 24 276.88 0.96 480 156 202 46 210.31 0.04 481 157 198 41 214.36 0.70 482 157 200 43 213.66 0.68 483 158 179 21 276.85 0.93 484 158 182 24 276.45 0.90 485 160 165 5 456.06 0.81 486 160 166 6 456.06 0.81 487 160 182 22 276.33 0.91 488 161 164 3 795.38 8.65 489 161 165 4 679.95 2.21 490 162 165 3 808.08 0.28 491 166 182 16 276.39 0.91 492 166 185 19 264.63 0.26 493 166 186 20 264.13 0.21 494 166 189 23 262.96 0.54 495 167 182 15 276.44 0.89 496 167 185 18 264.68 0.28 497 168 178 10 277.72 0.95 498 168 181 13 276.70 0.90 499 168 182 14 276.55 0.85 500 169 172 3 822.63 2.12 501 169 181 12 276.75 0.92 502 169 185 16 276.30 0.92 503 169 222 53 135.41 0.30 504 171 222 51 136.25 0.35 505 172 181 9 278.29 0.74 506 172 215 43 158.94 0.24 507 172 218 46 142.85 0.42 508 172 219 47 136.66 0.38 509 172 222 50 136.29 0.34 510 172 223 51 136.28 0.34 511 173 214 41 159.04 0.20 512 173 215 42 158.94 0.24 513 174 215 41 158.94 0.24 514 175 178 3 861.09 1.36 515 175 215 40 158.94 0.24 516 176 207 31 159.65 0.33 517 176 209 33 159.63 0.33 518 176 211 35 159.21 0.20 519 176 212 36 159.15 0.21 520 176 215 39 158.94 0.24 521 177 180 3 804.55 1.99 522 177 181 4 784.62 2.39 523 177 212 35 159.15 0.21 524 177 215 38 158.91 0.24 525 177 216 39 158.41 0.37 526 177 219 42 136.41 0.31 527 177 234 57 89.28 0.58 528 177 239 62 80.64 0.65 529 178 181 3 791.67 1.12 530 178 182 4 783.13 1.51 531 179 182 3 799.94 6.58 532 179 183 4 788.93 1.07 533 180 183 3 798.35 1.36 534 180 184 4 792.84 0.42 535 180 219 39 136.40 0.30 536 180 232 52 90.03 0.88 537 180 234 54 89.20 0.54 538 181 184 3 830.05 0.17 539 181 185 4 792.98 0.47 540 181 219 38 136.39 0.30 541 181 222 41 135.34 0.28 542 181 223 42 135.08 0.23 543 182 185 3 809.47 4.33 544 182 186 4 793.03 0.44 545 183 186 3 801.06 3.54 546 183 187 4 792.96 0.40 547 183 193 10 249.00 0.93 548 184 187 3 800.95 3.59 549 184 188 4 792.96 0.40 550 184 219 35 136.41 0.30 551 184 223 39 135.07 0.23 552 184 227 43 110.81 0.96 553 184 230 46 104.08 0.09 554 184 232 48 90.67 0.56 555 185 188 3 800.95 3.59 556 185 189 4 792.96 0.41 557 185 222 37 135.34 0.27 558 185 223 38 135.08 0.22 559 186 189 3 792.62 0.58 560 186 190 4 792.62 0.58 561 186 191 5 562.52 4.92 562 187 190 3 792.18 0.35 563 187 191 4 726.20 25.74 564 187 227 40 110.86 0.96 565 188 223 35 135.11 0.21 566 188 226 38 112.34 0.73 567 188 227 39 110.92 0.95 568 207 212 5 784.31 3.43 569 208 212 4 784.32 3.43 570 208 213 5 784.32 3.43 571 209 212 3 800.74 3.62 572 209 213 4 785.73 3.88 573 210 213 3 800.74 3.62 574 210 214 4 785.74 3.88 575 211 214 3 803.44 2.27 576 211 215 4 785.50 4.00 577 212 215 3 806.85 2.23 578 212 216 4 790.59 0.30 579 212 236 24 89.04 0.63 580 213 216 3 796.19 3.10 581 213 217 4 790.59 0.30 582 214 217 3 805.63 2.75 583 214 218 4 778.75 0.58 584 215 218 3 778.21 0.32 585 215 219 4 778.07 0.24 586 216 219 3 797.28 6.94 587 216 220 4 790.94 0.98 588 216 239 23 81.33 0.37 589 217 220 3 803.04 7.03 590 217 221 4 790.99 1.01 591 218 221 3 792.27 1.64 592 218 222 4 791.00 1.01 593 219 222 3 819.21 3.27 594 219 223 4 793.94 0.53 595 219 232 13 93.36 0.31 596 220 223 3 819.63 3.06 597 220 224 4 803.09 5.16 598 220 239 19 81.38 0.39 599 220 241 21 79.80 0.32 600 221 224 3 826.09 0.17 601 221 225 4 824.38 0.70 602 222 225 3 846.25 3.38 603 222 226 4 832.44 3.53 604 223 226 3 827.77 1.20 605 223 227 4 811.62 0.92 606 223 241 18 79.82 0.31 607 224 227 3 811.57 0.94 608 224 228 4 782.95 3.46 609 224 241 17 79.84 0.33 610 224 242 18 79.81 0.32 611 224 243 19 74.75 0.27 612 227 230 3 826.30 0.42 613 227 241 14 80.11 0.46 614 228 242 14 79.89 0.35 615 228 243 15 78.75 0.25 616 228 244 16 77.27 0.99 617 229 242 13 80.11 0.46 618 229 243 14 79.81 0.31 619 229 246 17 55.12 0.29 620 230 240 10 81.36 0.39 621 230 241 11 80.45 0.55 622 231 238 7 88.28 0.36 623 231 239 8 86.33 0.41 624 231 240 9 80.62 0.70 625 232 238 6 86.89 0.13 626 232 239 7 81.39 0.39 627 232 241 9 80.59 0.70 628 233 238 5 83.04 1.21 629 234 237 3 731.26 22.53 630 242 246 4 615.14 26.00 631 242 247 5 536.86 0.90 632 243 246 3 822.14 2.62 633 243 247 4 692.66 59.88 634 244 247 3 824.07 3.10 635 245 248 3 823.60 3.34