BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 237 235 38.48 0.17 2 3 235 232 47.69 0.28 3 3 236 233 42.55 0.78 4 4 54 50 66.89 0.11 5 4 55 51 65.50 0.24 6 4 56 52 61.91 1.31 7 4 234 230 60.89 0.80 8 4 235 231 56.84 0.30 9 5 233 228 63.86 0.50 10 5 234 229 61.20 0.95 11 6 52 46 75.22 1.39 12 6 213 207 72.41 2.79 13 6 231 225 78.03 0.07 14 6 232 226 68.08 0.09 15 6 233 227 65.54 0.27 16 7 231 224 78.05 0.07 17 7 232 225 71.93 1.16 18 8 11 3 831.22 3.91 19 8 15 7 703.90 12.53 20 8 18 10 395.95 149.60 21 8 52 44 80.16 1.14 22 8 230 222 79.59 0.80 23 8 231 223 78.03 0.07 24 9 230 221 83.84 0.04 25 10 29 19 104.31 1.61 26 10 229 219 95.94 1.06 27 11 15 4 815.75 1.29 28 11 18 7 700.95 16.27 29 11 26 15 107.42 0.14 30 11 29 18 104.33 1.61 31 11 30 19 99.55 0.78 32 11 31 20 104.55 1.50 33 11 228 217 99.53 0.78 34 11 229 218 98.28 0.24 35 11 230 219 98.09 0.32 36 11 231 220 97.81 0.19 37 12 15 3 845.63 2.40 38 12 26 14 110.25 0.06 39 13 26 13 112.05 0.85 40 15 18 3 837.01 1.11 41 15 20 5 803.99 0.98 42 15 26 11 120.46 0.95 43 17 52 35 117.47 0.04 44 17 53 36 114.77 0.69 45 18 51 33 118.04 0.13 46 18 52 34 117.62 0.01 47 18 231 213 118.08 0.14 48 19 50 31 121.41 0.23 49 19 51 32 120.07 0.88 50 19 53 34 118.06 0.12 51 19 212 193 120.06 0.87 52 20 24 4 711.45 16.13 53 20 25 5 703.02 18.01 54 20 26 6 703.35 17.85 55 20 49 29 135.14 0.40 56 20 50 30 129.91 0.64 57 21 24 3 825.60 4.46 58 21 48 27 143.51 0.82 59 21 49 28 135.68 0.66 60 21 51 30 135.69 0.66 61 21 97 76 137.69 0.32 62 21 98 77 137.68 0.32 63 21 99 78 137.73 0.31 64 22 47 25 150.40 1.16 65 22 48 26 146.84 0.24 66 22 99 77 147.01 0.32 67 23 33 10 150.50 1.20 68 23 35 12 150.76 1.14 69 23 46 23 150.75 1.13 70 23 47 24 150.52 1.18 71 23 48 25 150.42 1.18 72 24 32 8 415.16 131.06 73 24 33 9 150.50 1.20 74 24 48 24 150.50 1.20 75 24 49 25 150.46 1.18 76 24 50 26 150.44 1.18 77 25 31 6 589.39 50.12 78 25 32 7 416.19 131.55 79 26 30 4 756.49 11.38 80 26 31 5 606.98 42.74 81 27 30 3 804.55 2.39 82 27 32 5 687.38 19.73 83 27 36 9 913.37 20.54 84 30 227 197 192.83 0.62 85 30 228 198 172.97 0.35 86 30 229 199 169.75 0.94 87 31 50 19 742.53 23.30 88 31 226 195 194.93 1.26 89 31 227 196 193.03 0.70 90 31 228 197 185.79 0.68 91 32 225 193 213.33 0.01 92 32 226 194 193.62 0.61 93 32 227 195 193.30 0.77 94 33 49 16 905.58 0.09 95 33 50 17 846.40 0.06 96 33 88 55 227.38 7.74 97 33 215 182 239.22 2.42 98 33 216 183 238.86 2.43 99 33 217 184 230.98 3.94 100 33 226 193 210.27 0.07 101 34 217 183 238.49 2.29 102 34 218 184 237.59 1.90 103 34 219 185 236.33 1.33 104 34 224 190 223.75 0.32 105 34 225 191 219.78 0.39 106 34 226 192 219.53 0.51 107 36 225 189 229.06 0.34 108 41 80 39 238.88 2.58 109 42 78 36 238.88 2.60 110 42 79 37 238.84 2.60 111 42 80 38 238.90 2.58 112 42 223 181 230.83 0.43 113 42 224 182 230.73 0.41 114 42 225 183 230.69 0.39 115 43 219 176 239.06 2.40 116 43 223 180 230.83 0.44 117 44 220 176 238.53 2.25 118 44 221 177 237.66 1.89 119 44 223 179 230.83 0.44 120 45 219 174 238.74 2.19 121 45 220 175 237.67 1.89 122 46 218 172 238.78 2.19 123 46 219 173 238.63 2.14 124 47 99 52 239.64 2.29 125 47 104 57 238.68 2.12 126 47 109 62 238.87 2.16 127 47 217 170 239.12 2.31 128 47 218 171 238.56 2.16 129 48 216 168 239.21 2.35 130 48 217 169 239.05 2.32 131 49 91 42 239.78 2.34 132 49 97 48 239.91 2.37 133 49 98 49 239.78 2.34 134 49 99 50 239.69 2.31 135 49 100 51 239.72 2.30 136 49 109 60 239.53 2.27 137 49 110 61 239.62 2.29 138 49 215 166 239.61 2.31 139 49 216 167 239.28 2.37 140 50 214 164 239.79 2.34 141 50 215 165 239.68 2.31 142 50 228 178 221.72 0.33 143 51 91 40 239.88 2.35 144 51 97 46 239.97 2.34 145 51 212 161 239.97 2.34 146 51 213 162 239.91 2.36 147 51 214 163 239.83 2.36 148 52 212 160 240.02 2.34 149 52 213 161 239.98 2.33 150 52 231 179 219.50 0.49 151 53 212 159 242.62 1.44 152 55 95 40 242.89 1.38 153 55 210 155 249.47 3.00 154 55 211 156 242.90 1.38 155 55 212 157 242.72 1.45 156 56 210 154 249.64 3.06 157 56 211 155 247.34 2.69 158 56 213 157 242.99 1.43 159 56 233 177 218.62 0.33 160 56 235 179 212.88 0.32 161 57 60 3 875.42 4.38 162 57 67 10 259.23 0.35 163 57 209 152 258.91 0.49 164 57 210 153 251.19 3.83 165 57 237 180 212.75 0.38 166 58 66 8 259.61 0.40 167 58 67 9 259.34 0.29 168 58 69 11 259.20 0.35 169 58 203 145 259.52 0.38 170 58 207 149 259.22 0.43 171 58 209 151 259.23 0.42 172 58 211 153 258.78 0.54 173 58 237 179 212.77 0.38 174 59 65 6 454.44 96.97 175 59 66 7 259.58 0.41 176 59 204 145 259.57 0.39 177 59 207 148 259.55 0.40 178 59 208 149 259.54 0.41 179 59 209 150 259.35 0.47 180 59 237 178 212.77 0.37 181 60 63 3 817.55 2.68 182 60 64 4 732.82 24.34 183 60 65 5 652.90 39.37 184 60 209 149 259.61 0.41 185 60 237 177 212.97 0.29 186 61 64 3 784.45 4.42 187 61 209 148 259.68 0.42 188 63 239 176 212.88 0.28 189 65 237 172 212.97 0.33 190 65 238 173 212.78 0.37 191 65 239 174 212.71 0.36 192 66 176 110 343.75 0.45 193 66 178 112 342.74 0.03 194 66 203 137 325.78 3.27 195 66 204 138 325.85 3.23 196 66 237 171 212.96 0.33 197 66 238 172 212.75 0.38 198 67 176 109 343.76 0.45 199 67 178 111 342.82 0.04 200 67 235 168 216.40 0.24 201 67 236 169 216.11 0.12 202 67 237 170 212.75 0.38 203 68 176 108 343.76 0.45 204 68 178 110 342.82 0.04 205 68 235 167 217.40 0.71 206 68 236 168 216.41 0.24 207 68 238 170 212.75 0.38 208 69 92 23 341.69 0.12 209 69 174 105 345.06 0.43 210 69 175 106 344.71 0.31 211 69 176 107 344.00 0.34 212 69 233 164 218.83 0.34 213 69 234 165 218.63 0.35 214 69 235 166 216.16 0.10 215 70 173 103 345.19 0.39 216 70 174 104 345.04 0.44 217 70 175 105 344.72 0.31 218 70 233 163 218.75 0.38 219 70 234 164 218.66 0.34 220 71 90 19 342.50 0.10 221 71 173 102 345.24 0.38 222 71 174 103 345.12 0.42 223 71 231 160 220.62 0.46 224 71 232 161 219.78 0.38 225 71 233 162 218.75 0.38 226 72 172 100 345.49 0.42 227 72 173 101 345.30 0.41 228 72 231 159 220.70 0.50 229 72 232 160 220.18 0.46 230 73 90 17 344.44 0.42 231 73 129 56 345.29 0.33 232 73 171 98 346.23 0.26 233 73 172 99 345.71 0.48 234 73 174 101 345.75 0.49 235 73 215 142 343.70 0.12 236 73 228 155 229.74 0.34 237 73 230 157 222.65 0.42 238 73 231 158 220.62 0.46 239 74 170 96 346.88 0.45 240 74 171 97 346.25 0.27 241 74 228 154 230.04 0.20 242 74 229 155 229.72 0.34 243 74 230 156 228.90 0.29 244 75 88 13 344.89 0.45 245 75 129 54 345.40 0.38 246 75 162 87 350.37 0.02 247 75 169 94 348.05 0.42 248 75 170 95 347.94 0.39 249 75 172 97 347.60 0.23 250 75 227 152 230.15 0.18 251 75 228 153 229.78 0.35 252 75 229 154 229.74 0.34 253 76 168 92 348.56 0.19 254 76 169 93 348.06 0.42 255 76 226 150 273.12 3.30 256 76 227 151 248.51 8.67 257 76 229 153 230.15 0.18 258 77 88 11 345.54 0.47 259 77 131 54 345.39 0.38 260 77 162 85 355.65 2.55 261 77 164 87 350.48 0.03 262 77 166 89 355.70 2.52 263 77 167 90 350.53 0.03 264 77 224 147 311.57 0.54 265 77 225 148 308.25 0.01 266 77 226 149 273.12 3.30 267 78 164 86 378.26 3.08 268 78 166 88 355.71 2.53 269 78 167 89 350.62 0.03 270 78 224 146 311.75 0.59 271 78 225 147 311.37 0.64 272 79 84 5 345.28 0.33 273 79 164 85 391.70 0.24 274 79 166 87 376.97 3.70 275 79 223 144 312.67 0.23 276 79 224 145 311.79 0.61 277 80 223 143 332.15 0.29 278 83 222 139 341.27 0.03 279 84 131 47 551.11 43.98 280 84 164 80 462.28 0.03 281 84 223 139 312.56 0.29 282 84 224 140 311.81 0.61 283 85 131 46 551.22 44.01 284 85 132 47 551.07 43.93 285 85 218 133 348.86 0.26 286 85 219 134 346.87 0.88 287 85 222 137 314.54 0.33 288 85 223 138 311.83 0.61 289 85 224 139 311.81 0.61 290 85 226 141 311.80 0.61 291 86 105 19 484.74 9.73 292 86 106 20 484.68 9.76 293 86 108 22 484.70 9.73 294 86 130 44 551.24 44.02 295 86 131 45 551.25 44.01 296 86 132 46 551.20 43.95 297 86 218 132 348.85 0.23 298 86 219 133 347.87 0.37 299 86 220 134 347.81 0.35 300 86 224 138 311.81 0.61 301 86 226 140 311.56 0.54 302 87 108 21 485.50 10.12 303 87 129 42 551.36 44.08 304 87 130 43 551.35 44.06 305 87 217 130 349.11 0.14 306 87 218 131 349.07 0.12 307 87 224 137 311.80 0.61 308 87 226 139 273.08 3.31 309 88 128 40 552.25 43.76 310 88 129 41 552.10 43.84 311 88 215 127 352.82 0.17 312 88 216 128 351.90 0.12 313 88 217 129 349.11 0.14 314 88 226 138 247.14 9.42 315 89 108 19 519.61 26.78 316 89 110 21 540.22 36.91 317 89 126 37 552.60 43.80 318 89 127 38 552.49 43.77 319 89 128 39 552.25 43.76 320 89 215 126 352.85 0.18 321 89 216 127 352.32 0.20 322 90 126 36 552.62 43.80 323 90 127 37 552.53 43.76 324 90 174 84 463.05 0.11 325 90 213 123 353.66 0.09 326 90 214 124 353.10 0.27 327 90 215 125 352.89 0.18 328 90 231 141 219.91 0.42 329 91 97 6 772.65 5.34 330 91 110 19 552.07 42.80 331 91 124 33 553.36 43.49 332 91 125 34 553.19 43.53 333 91 126 35 552.75 43.73 334 91 213 122 353.66 0.09 335 91 214 123 353.37 0.17 336 91 215 124 352.93 0.18 337 91 216 125 352.92 0.18 338 92 124 32 553.40 43.48 339 92 125 33 553.32 43.49 340 92 207 115 431.19 0.38 341 92 211 119 355.43 0.49 342 92 212 120 355.26 0.42 343 92 213 121 353.66 0.08 344 93 97 4 783.55 0.10 345 93 123 30 553.50 43.52 346 93 124 31 553.40 43.48 347 93 207 114 431.21 0.38 348 93 211 118 355.43 0.50 349 93 212 119 355.37 0.48 350 94 97 3 807.62 0.56 351 94 123 29 553.53 43.50 352 94 207 113 431.24 0.36 353 94 208 114 431.17 0.39 354 95 210 115 427.00 1.43 355 95 211 116 367.43 0.90 356 95 212 117 356.53 1.06 357 99 103 4 789.70 4.04 358 100 103 3 822.33 0.24 359 100 109 9 673.52 18.48 360 100 110 10 653.91 9.29 361 100 111 11 595.00 38.48 362 100 112 12 597.22 37.30 363 101 113 12 597.55 37.16 364 102 107 5 779.07 2.29 365 102 113 11 597.56 37.15 366 103 107 4 762.28 2.29 367 103 108 5 718.92 16.23 368 103 109 6 681.87 22.59 369 103 110 7 673.75 18.53 370 103 111 8 673.64 18.51 371 104 107 3 817.12 5.08 372 104 218 114 350.36 0.39 373 105 108 3 812.38 2.08 374 105 218 113 349.14 0.12 375 105 219 114 349.10 0.10 376 105 220 115 348.79 0.01 377 106 138 32 581.64 29.33 378 106 140 34 581.97 29.18 379 106 154 48 581.61 28.99 380 107 110 3 827.47 1.72 381 107 111 4 767.34 2.73 382 107 112 5 765.05 3.87 383 107 113 6 684.32 23.78 384 107 116 9 615.46 44.74 385 107 140 33 582.01 29.16 386 107 154 47 581.59 28.98 387 108 128 20 581.74 29.55 388 108 154 46 581.74 28.92 389 108 216 108 352.56 0.14 390 108 218 110 349.16 0.12 391 109 216 107 352.59 0.15 392 109 218 109 350.82 0.60 393 110 126 16 583.50 28.77 394 110 216 106 352.65 0.13 395 111 116 5 624.25 49.05 396 111 122 11 583.70 28.71 397 111 124 13 583.67 28.73 398 111 152 41 582.88 28.35 399 111 153 42 582.82 28.33 400 111 154 43 582.62 28.32 401 112 115 3 805.31 2.39 402 112 116 4 720.55 23.12 403 113 116 3 813.26 1.59 404 115 118 3 776.07 2.98 405 115 121 6 674.47 20.30 406 115 122 7 674.46 20.30 407 116 153 37 583.19 28.04 408 116 154 38 583.15 28.05 409 117 140 23 583.53 28.24 410 117 153 36 583.17 28.04 411 117 154 37 583.18 28.04 412 117 155 38 583.14 28.04 413 118 121 3 803.54 2.32 414 118 122 4 787.28 0.59 415 119 122 3 821.38 2.67 416 119 152 33 596.93 21.15 417 119 153 34 597.99 20.62 418 122 152 30 633.65 2.64 419 123 152 29 634.76 2.00 420 123 206 83 431.38 0.41 421 123 207 84 431.28 0.42 422 123 208 85 431.23 0.39 423 124 151 27 634.88 1.95 424 124 152 28 634.20 1.72 425 124 206 82 431.38 0.41 426 124 207 83 431.20 0.40 427 125 150 25 634.98 1.91 428 125 151 26 634.51 1.76 429 125 183 58 463.00 0.07 430 125 201 76 449.33 0.49 431 125 203 78 431.45 0.37 432 125 206 81 431.33 0.40 433 125 207 82 431.19 0.38 434 126 149 23 635.11 1.90 435 126 150 24 634.97 1.89 436 127 147 20 635.43 1.88 437 127 148 21 635.28 1.81 438 127 174 47 463.16 0.06 439 127 183 56 462.81 0.16 440 127 185 58 463.12 0.03 441 128 146 18 635.57 1.83 442 128 147 19 635.24 1.78 443 128 148 20 635.21 1.77 444 128 150 22 633.99 1.17 445 128 154 26 601.49 14.85 446 128 156 28 567.08 31.87 447 129 146 17 635.38 1.75 448 129 147 18 635.23 1.77 449 129 162 33 547.63 41.30 450 129 170 41 463.68 0.14 451 129 172 43 463.62 0.14 452 129 187 58 463.61 0.14 453 130 141 11 656.86 11.97 454 130 145 15 635.55 1.79 455 130 146 16 635.33 1.72 456 130 162 32 547.80 41.38 457 131 162 31 547.82 41.39 458 131 164 33 545.11 40.93 459 131 224 93 311.81 0.61 460 132 137 5 789.32 3.72 461 132 138 6 758.11 1.26 462 132 141 9 657.37 12.06 463 132 222 90 326.97 2.17 464 133 138 5 758.11 1.26 465 133 141 8 657.37 12.06 466 133 142 9 657.37 12.06 467 133 143 10 656.82 11.92 468 133 144 11 645.81 6.51 469 133 145 12 640.31 3.81 470 134 137 3 816.72 2.67 471 134 138 4 775.61 2.00 472 135 139 4 759.32 0.65 473 135 141 6 758.08 1.26 474 135 143 8 670.76 18.62 475 136 139 3 835.22 4.68 476 138 141 3 818.81 1.85 477 140 154 14 566.87 32.05 478 140 155 15 566.86 32.05 479 141 146 5 780.37 0.01 480 142 146 4 799.53 5.23 481 144 161 17 547.89 41.42 482 145 161 16 547.86 41.40 483 145 162 17 547.78 41.38 484 146 159 13 553.71 38.52 485 146 160 14 553.66 38.52 486 146 161 15 547.84 41.38 487 146 162 16 547.62 41.29 488 147 159 12 553.70 38.51 489 147 160 13 553.60 38.49 490 147 162 15 547.62 41.29 491 147 185 38 463.32 0.03 492 147 187 40 463.59 0.12 493 147 195 48 463.56 0.11 494 147 197 50 462.79 0.14 495 148 156 8 567.07 31.87 496 148 158 10 562.34 34.20 497 148 159 11 553.62 38.47 498 149 156 7 567.07 31.86 499 149 157 8 567.07 31.86 500 149 158 9 566.78 31.91 501 149 183 34 463.00 0.07 502 149 185 36 463.28 0.01 503 149 197 48 463.18 0.02 504 149 198 49 463.00 0.07 505 149 199 50 462.72 0.14 506 150 153 3 823.75 0.75 507 150 156 6 567.03 31.85 508 151 157 6 669.75 19.58 509 151 201 50 452.53 2.16 510 151 206 55 431.36 0.42 511 152 206 54 431.37 0.42 512 156 159 3 828.97 0.24 513 157 199 42 462.00 0.14 514 158 197 39 463.21 0.02 515 158 198 40 463.18 0.01 516 158 199 41 462.70 0.14 517 159 197 38 463.19 0.01 518 160 196 36 463.47 0.07 519 160 197 37 463.09 0.04 520 161 195 34 463.64 0.14 521 162 170 8 659.39 12.14 522 162 189 27 558.22 46.30 523 162 195 33 463.63 0.14 524 163 189 26 572.33 53.02 525 163 195 32 463.67 0.15 526 164 189 25 576.10 52.01 527 164 224 60 311.83 0.63 528 165 168 3 810.70 0.07 529 165 189 24 603.86 49.54 530 165 191 26 650.59 51.74 531 165 193 28 618.64 47.44 532 168 189 21 651.35 51.66 533 169 189 20 651.35 51.66 534 169 229 60 230.25 0.19 535 170 187 17 651.47 51.65 536 170 188 18 651.42 51.65 537 170 189 19 651.35 51.66 538 171 187 16 651.48 51.65 539 171 188 17 651.42 51.65 540 172 185 13 651.94 51.55 541 172 186 14 651.60 51.62 542 172 187 15 651.49 51.66 543 173 185 12 651.94 51.55 544 173 186 13 651.81 51.55 545 174 183 9 652.32 51.45 546 174 184 10 652.22 51.44 547 174 185 11 651.95 51.55 548 175 183 8 652.33 51.45 549 175 184 9 652.28 51.45 550 176 181 5 745.00 6.42 551 176 182 6 721.62 16.98 552 176 183 7 652.33 51.45 553 176 201 25 650.92 51.61 554 176 203 27 632.34 52.90 555 177 180 3 796.55 2.27 556 177 181 4 765.70 2.27 557 177 182 5 738.72 9.54 558 178 181 3 778.10 3.93 559 178 238 60 212.78 0.37 560 178 240 62 190.36 0.16 561 179 240 61 194.49 2.04 562 180 200 20 651.31 51.68 563 181 200 19 651.25 51.66 564 181 201 20 650.98 51.62 565 181 202 21 650.88 51.59 566 181 204 23 650.55 51.43 567 182 199 17 651.26 51.66 568 182 200 18 650.95 51.61 569 183 198 15 651.26 51.66 570 183 199 16 651.12 51.66 571 183 201 18 650.89 51.60 572 184 197 13 651.30 51.66 573 184 198 14 650.99 51.65 574 185 196 11 651.31 51.66 575 185 197 12 651.19 51.69 576 186 194 8 651.35 51.66 577 186 195 9 651.30 51.66 578 186 196 10 651.14 51.65 579 187 194 7 651.30 51.66 580 187 195 8 651.27 51.65 581 188 193 5 757.85 1.61 582 188 194 6 702.44 26.00 583 189 192 3 810.73 2.49 584 189 193 4 759.67 0.71 585 189 195 6 761.60 3.51 586 201 206 5 785.88 0.65 587 202 205 3 826.43 0.07 588 202 206 4 791.30 1.08 589 203 206 3 804.72 3.75 590 203 207 4 784.34 1.42 591 207 211 4 810.13 2.25 592 208 211 3 850.91 2.21 593 213 231 18 219.40 0.20 594 213 233 20 216.28 0.30 595 214 231 17 219.25 0.12 596 215 228 13 222.22 0.47 597 215 231 16 218.81 0.35 598 217 226 9 246.63 9.67 599 218 226 8 300.00 0.52 600 219 222 3 819.75 2.41 601 219 223 4 696.17 27.93 602 219 224 5 696.17 27.93 603 219 226 7 505.84 75.47