BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 22 21 209.36 0.60 2 1 23 22 208.84 0.41 3 2 22 20 210.79 1.24 4 2 23 21 209.43 0.58 5 2 24 22 209.07 0.67 6 2 57 55 226.13 0.54 7 2 58 56 226.12 0.54 8 2 146 144 212.22 0.83 9 2 147 145 209.59 0.55 10 2 179 177 209.41 0.57 11 3 20 17 213.45 0.40 12 3 21 18 213.23 0.41 13 3 22 19 211.06 1.22 14 3 44 41 212.97 0.30 15 3 55 52 226.29 0.57 16 3 56 53 226.24 0.54 17 3 57 54 226.13 0.54 18 3 147 144 211.19 1.25 19 3 181 178 209.48 0.53 20 4 20 16 213.45 0.40 21 4 21 17 213.24 0.42 22 4 55 51 226.29 0.57 23 4 56 52 226.29 0.57 24 5 18 13 217.87 1.21 25 5 19 14 215.41 0.46 26 5 20 15 213.45 0.40 27 5 40 35 213.74 0.51 28 5 44 39 213.81 0.54 29 5 54 49 231.59 1.10 30 5 55 50 226.30 0.57 31 6 18 12 217.88 1.20 32 6 19 13 215.41 0.46 33 6 52 46 237.07 0.66 34 6 53 47 234.87 0.11 35 6 54 48 234.41 0.31 36 7 16 9 234.19 0.42 37 7 17 10 226.03 1.56 38 7 18 11 224.03 1.52 39 7 50 43 236.53 0.39 40 7 52 45 237.06 0.66 41 7 53 46 234.91 0.12 42 7 54 47 234.86 0.11 43 8 16 8 234.13 0.41 44 8 17 9 228.76 0.20 45 8 50 42 237.09 0.65 46 8 51 43 237.09 0.65 47 8 52 44 237.08 0.66 48 9 15 6 272.57 18.57 49 9 16 7 235.04 0.18 50 9 48 39 237.09 0.65 51 9 49 40 237.09 0.66 52 9 50 41 237.09 0.66 53 9 51 42 237.11 0.65 54 10 14 4 600.24 18.87 55 10 15 5 406.19 85.36 56 10 51 41 237.16 0.62 57 11 14 3 758.37 10.37 58 11 39 28 235.83 0.21 59 14 35 21 235.86 0.24 60 14 36 22 236.55 0.45 61 14 39 25 237.17 0.60 62 14 202 188 166.96 2.79 63 14 203 189 166.50 3.15 64 15 202 187 166.51 3.15 65 15 203 188 161.56 5.62 66 16 39 23 237.18 0.59 67 16 40 24 237.18 0.59 68 16 43 27 239.72 0.08 69 16 200 184 167.53 2.87 70 16 203 187 152.29 10.26 71 17 203 186 152.38 10.30 72 17 204 187 148.19 12.35 73 18 40 22 239.58 0.17 74 18 203 185 154.15 9.45 75 18 205 187 145.75 13.58 76 20 27 7 331.51 1.35 77 20 40 20 241.15 0.27 78 20 181 161 241.25 0.26 79 20 198 178 167.02 3.46 80 20 205 185 145.75 13.62 81 20 208 188 142.69 15.06 82 21 208 187 142.72 15.09 83 22 25 3 985.03 1.22 84 22 27 5 555.59 10.74 85 22 147 125 304.76 0.48 86 22 179 157 304.75 0.48 87 22 180 158 299.42 1.20 88 22 181 159 241.33 0.30 89 22 208 186 142.74 15.09 90 23 196 173 169.81 2.38 91 23 208 185 142.77 15.09 92 24 179 155 304.78 0.48 93 25 173 148 304.93 0.52 94 25 179 154 304.82 0.48 95 25 180 155 304.82 0.48 96 25 195 170 170.07 2.49 97 26 169 143 304.91 0.52 98 26 173 147 304.95 0.52 99 26 180 154 304.83 0.48 100 26 182 156 304.79 0.48 101 26 193 167 172.26 3.37 102 26 194 168 170.12 2.48 103 26 195 169 170.07 2.50 104 26 196 170 170.06 2.49 105 27 180 153 304.92 0.51 106 27 181 154 304.83 0.48 107 27 182 155 304.79 0.49 108 27 196 169 169.75 2.37 109 27 208 181 142.77 15.08 110 28 182 154 304.80 0.49 111 28 190 162 175.94 4.87 112 28 193 165 173.44 3.79 113 28 194 166 170.15 2.46 114 28 196 168 169.81 2.35 115 28 197 169 169.52 2.28 116 28 198 170 169.47 2.31 117 29 37 8 319.92 1.40 118 29 40 11 318.63 2.04 119 29 181 152 304.96 0.52 120 29 182 153 304.89 0.49 121 29 183 154 304.80 0.49 122 29 184 155 304.79 0.48 123 29 198 169 169.40 2.27 124 29 200 171 169.30 2.23 125 29 205 176 146.02 13.56 126 30 184 154 304.82 0.48 127 30 185 155 304.78 0.48 128 30 186 156 304.75 0.48 129 30 187 157 293.55 1.70 130 30 190 160 218.68 16.16 131 30 197 167 169.62 2.25 132 30 198 168 169.54 2.25 133 30 199 169 169.31 2.23 134 30 200 170 169.30 2.23 135 30 211 181 135.25 11.33 136 30 225 195 61.34 0.21 137 30 226 196 61.25 0.20 138 30 229 199 58.54 0.72 139 31 35 4 744.08 0.56 140 31 37 6 443.02 48.06 141 31 184 153 304.88 0.49 142 31 185 154 304.80 0.49 143 31 200 169 169.16 2.17 144 31 225 194 61.34 0.21 145 32 35 3 811.47 3.29 146 32 37 5 754.90 3.45 147 32 185 153 304.86 0.48 148 32 200 168 169.38 2.19 149 32 201 169 169.17 2.17 150 32 225 193 61.37 0.21 151 35 200 165 168.88 2.28 152 35 201 166 167.78 2.75 153 35 202 167 167.03 3.03 154 35 203 168 166.59 3.19 155 36 39 3 815.40 3.70 156 36 40 4 788.46 0.36 157 36 200 164 168.88 2.29 158 37 40 3 794.36 2.58 159 37 41 4 801.25 6.04 160 37 152 115 305.31 0.55 161 37 183 146 305.30 0.55 162 37 184 147 305.28 0.55 163 37 185 148 305.25 0.56 164 37 200 163 168.46 2.45 165 38 41 3 813.41 0.04 166 38 42 4 788.48 0.35 167 38 151 113 305.33 0.54 168 39 42 3 804.25 4.54 169 39 43 4 776.89 5.44 170 40 43 3 801.79 5.77 171 40 44 4 801.16 6.08 172 40 181 141 312.14 2.79 173 40 200 160 167.70 2.83 174 40 205 165 145.91 13.51 175 41 44 3 793.56 1.83 176 41 45 4 774.29 4.17 177 41 74 33 312.14 2.79 178 41 149 108 312.12 2.78 179 41 150 109 312.11 2.78 180 41 151 110 311.68 2.75 181 41 181 140 312.14 2.79 182 41 183 142 309.90 2.19 183 42 45 3 784.70 0.96 184 42 46 4 772.22 5.21 185 42 47 5 611.86 18.45 186 42 48 6 606.53 17.37 187 42 74 32 312.15 2.79 188 43 46 3 792.13 2.76 189 43 48 5 573.29 0.74 190 43 50 7 434.62 51.56 191 43 55 12 314.20 3.08 192 43 70 27 314.28 3.14 193 44 55 11 314.18 3.07 194 44 70 26 314.28 3.13 195 44 72 28 312.47 2.69 196 44 147 103 313.17 3.04 197 44 149 105 312.15 2.79 198 45 67 22 314.33 3.15 199 45 70 25 314.25 3.12 200 45 72 27 312.48 2.69 201 45 74 29 312.16 2.78 202 45 149 104 312.21 2.79 203 46 67 21 314.33 3.16 204 46 70 24 314.28 3.14 205 47 67 20 314.33 3.17 206 50 53 3 854.11 4.39 207 50 63 13 314.84 3.10 208 54 61 7 318.87 1.69 209 54 63 9 316.39 2.69 210 55 62 7 317.35 2.43 211 55 63 8 316.43 2.67 212 56 60 4 331.03 1.67 213 56 61 5 318.91 1.69 214 57 60 3 793.50 4.18 215 57 62 5 333.76 4.49 216 57 146 89 316.05 2.52 217 57 147 90 316.01 2.51 218 58 146 88 316.06 2.52 219 61 64 3 897.78 4.88 220 62 65 3 820.27 1.71 221 62 70 8 431.31 51.77 222 62 146 84 325.76 1.02 223 63 70 7 537.35 0.55 224 65 69 4 775.52 5.46 225 65 70 5 742.24 5.40 226 66 69 3 806.01 1.66 227 66 70 4 775.52 5.46 228 67 70 3 806.01 1.66 229 71 145 74 443.54 2.48 230 71 146 75 443.44 2.48 231 72 147 75 443.44 2.48 232 72 149 77 443.49 2.46 233 73 145 72 443.61 2.47 234 73 147 74 443.56 2.48 235 73 148 75 443.50 2.46 236 73 149 76 443.50 2.46 237 74 149 75 443.52 2.46 238 75 79 4 641.75 4.19 239 75 140 65 443.81 2.46 240 75 149 74 443.59 2.48 241 75 150 75 443.56 2.48 242 75 151 76 443.56 2.48 243 76 79 3 793.63 6.61 244 76 80 4 687.02 26.82 245 76 151 75 443.60 2.46 246 77 151 74 443.60 2.46 247 77 152 75 443.60 2.46 248 79 132 53 479.17 0.29 249 79 133 54 478.99 0.24 250 79 136 57 478.55 0.45 251 79 153 74 443.68 2.44 252 80 139 59 479.17 0.26 253 81 90 9 531.26 1.66 254 81 124 43 506.39 0.45 255 81 127 46 516.85 3.25 256 81 132 51 480.25 0.56 257 81 135 54 479.39 0.20 258 81 136 55 479.38 0.20 259 81 139 58 479.38 0.20 260 82 85 3 799.04 0.46 261 82 90 8 531.74 1.42 262 82 93 11 531.78 1.40 263 82 124 42 524.93 0.77 264 82 139 57 479.49 0.17 265 83 124 41 524.93 0.77 266 85 89 4 767.31 7.35 267 85 90 5 767.35 7.33 268 85 93 8 601.75 3.94 269 85 123 38 530.05 1.34 270 85 124 39 530.04 1.34 271 86 89 3 792.43 3.08 272 86 90 4 787.46 2.66 273 86 120 34 531.00 1.09 274 86 121 35 530.35 1.33 275 86 122 36 530.18 1.40 276 86 123 37 530.05 1.34 277 87 90 3 806.44 0.32 278 87 91 4 787.49 2.65 279 87 116 29 531.42 1.03 280 87 119 32 531.04 1.08 281 87 120 33 531.00 1.09 282 87 122 35 530.89 1.06 283 87 127 40 530.06 1.34 284 87 135 48 527.34 0.83 285 88 91 3 798.90 4.50 286 88 92 4 787.50 2.66 287 88 104 16 532.42 0.75 288 88 107 19 532.37 0.73 289 88 108 20 532.39 0.74 290 88 120 32 531.02 1.11 291 89 92 3 789.33 3.57 292 89 93 4 787.50 2.66 293 90 93 3 793.58 1.83 294 90 124 34 530.14 1.34 295 90 135 45 527.36 0.84 296 90 139 49 527.01 0.88 297 91 107 16 532.39 0.74 298 91 111 20 532.37 0.73 299 91 116 25 531.50 1.06 300 91 120 29 531.09 1.12 301 91 135 44 527.38 0.85 302 91 138 47 527.26 0.84 303 91 139 48 527.01 0.88 304 91 142 51 526.41 1.06 305 92 95 3 821.78 1.03 306 92 107 15 532.40 0.75 307 94 143 49 526.34 1.09 308 95 98 3 789.43 3.53 309 95 103 8 532.84 0.82 310 95 106 11 534.11 1.54 311 95 107 12 532.74 0.85 312 95 142 47 526.49 1.10 313 95 143 48 526.35 1.09 314 96 103 7 635.24 2.76 315 98 102 4 787.50 1.78 316 98 103 5 769.28 3.17 317 98 106 8 762.25 5.37 318 99 102 3 802.50 1.01 319 99 103 4 780.11 1.83 320 100 103 3 799.23 1.27 321 100 104 4 785.76 0.97 322 101 104 3 804.56 1.39 323 101 105 4 790.24 3.21 324 102 105 3 792.19 2.24 325 102 106 4 790.24 3.21 326 103 106 3 814.52 6.38 327 103 107 4 791.31 2.67 328 104 107 3 798.04 0.69 329 104 108 4 790.08 2.05 330 105 108 3 812.79 0.38 331 105 109 4 788.56 1.29 332 106 109 3 796.80 1.30 333 106 110 4 788.56 1.29 334 106 142 36 526.96 0.86 335 106 144 38 526.90 0.84 336 107 110 3 800.82 1.54 337 107 111 4 783.15 0.38 338 107 120 13 531.45 1.03 339 108 111 3 789.53 3.59 340 108 112 4 788.23 2.94 341 108 120 12 531.52 1.06 342 109 112 3 801.96 0.08 343 109 113 4 788.24 2.94 344 110 113 3 788.24 2.94 345 110 114 4 788.24 2.94 346 110 138 28 527.35 0.88 347 110 141 31 527.35 0.87 348 110 142 32 527.04 0.86 349 110 176 66 525.45 1.31 350 111 114 3 803.07 3.51 351 111 115 4 788.23 2.94 352 111 116 5 648.32 3.46 353 111 117 6 559.46 12.87 354 111 120 9 536.02 1.17 355 112 115 3 775.80 3.27 356 112 117 5 584.70 11.60 357 113 176 63 525.62 1.40 358 114 134 20 529.62 1.26 359 114 137 23 529.45 1.26 360 114 138 24 528.48 0.89 361 114 141 27 528.16 0.83 362 114 172 58 524.83 1.50 363 115 118 3 896.09 3.97 364 115 134 19 529.71 1.25 365 116 119 3 807.38 3.08 366 116 120 4 791.15 1.64 367 116 134 18 529.68 1.24 368 116 135 19 529.62 1.22 369 116 138 22 528.35 0.81 370 117 120 3 804.45 5.01 371 119 122 3 783.73 0.12 372 119 127 8 530.75 1.47 373 119 131 12 529.90 1.30 374 122 126 4 775.87 2.86 375 122 127 5 743.34 2.39 376 123 126 3 809.81 0.75 377 123 127 4 795.91 7.70 378 124 127 3 802.77 1.62 379 124 132 8 573.83 5.45 380 125 132 7 748.50 4.96 381 127 131 4 769.73 0.69 382 127 132 5 755.32 6.24 383 127 135 8 750.62 3.90 384 128 131 3 779.97 1.28 385 128 132 4 779.99 1.31 386 129 132 3 796.59 5.15 387 129 133 4 799.19 4.42 388 130 133 3 805.46 1.29 389 130 134 4 800.18 3.94 390 131 134 3 802.80 2.62 391 131 135 4 800.20 3.92 392 132 135 3 802.12 2.96 393 132 136 4 800.20 3.92 394 133 136 3 793.76 0.68 395 133 137 4 786.67 2.87 396 133 165 32 474.57 12.64 397 134 137 3 795.97 1.76 398 134 138 4 786.67 2.87 399 134 168 34 520.88 1.91 400 135 138 3 788.35 2.04 401 135 139 4 786.70 2.85 402 136 139 3 807.30 4.66 403 136 140 4 786.74 2.84 404 137 140 3 795.96 1.77 405 137 141 4 783.15 1.04 406 137 148 11 498.80 0.60 407 137 169 32 518.61 0.04 408 137 172 35 524.84 1.51 409 138 141 3 802.78 1.01 410 138 142 4 793.62 2.94 411 139 142 3 814.60 3.40 412 140 145 5 750.87 2.85 413 140 148 8 474.32 12.58 414 141 144 3 785.20 2.43 415 141 145 4 752.34 1.46 416 141 148 7 546.85 1.30 417 141 172 31 599.19 24.76 418 141 176 35 609.33 19.71 419 141 178 37 592.07 11.09 420 145 148 3 838.51 0.18 421 145 178 33 730.32 2.04 422 146 179 33 730.39 2.02 423 147 178 31 730.42 2.02 424 147 179 32 730.39 2.02 425 147 180 33 730.36 2.04 426 147 181 34 730.35 2.03 427 148 178 30 730.49 2.01 428 148 179 31 730.44 2.00 429 148 180 32 730.35 2.03 430 148 181 33 730.35 2.03 431 149 181 32 730.36 2.04 432 150 153 3 825.82 0.21 433 150 169 19 730.59 2.02 434 150 180 30 730.54 2.02 435 150 181 31 730.43 2.02 436 150 182 32 730.36 2.03 437 150 183 33 730.32 2.05 438 151 183 32 730.35 2.04 439 152 155 3 776.20 3.58 440 152 183 31 730.43 2.02 441 152 184 32 730.37 2.03 442 152 185 33 730.38 2.03 443 153 182 29 730.54 2.02 444 154 157 3 814.94 1.07 445 154 162 8 730.74 1.99 446 154 165 11 730.75 2.00 447 154 166 12 730.72 1.99 448 154 169 15 730.74 2.00 449 154 182 28 730.59 2.02 450 154 189 35 696.78 2.33 451 157 161 4 785.90 0.78 452 157 162 5 775.41 0.91 453 157 165 8 762.24 3.18 454 158 161 3 790.11 0.38 455 158 162 4 786.51 0.48 456 159 162 3 793.00 1.18 457 159 163 4 789.12 0.81 458 159 188 29 730.50 1.98 459 160 163 3 820.35 1.04 460 160 164 4 808.28 2.06 461 161 164 3 806.25 5.45 462 161 165 4 789.12 0.81 463 162 165 3 786.57 2.66 464 162 166 4 783.81 2.85 465 162 186 24 730.60 2.02 466 162 188 26 729.85 1.88 467 162 189 27 718.93 5.99 468 163 166 3 797.84 3.66 469 163 167 4 783.80 2.85 470 164 167 3 815.11 4.67 471 164 168 4 787.74 0.90 472 165 168 3 793.37 0.47 473 165 169 4 787.72 0.89 474 166 169 3 788.90 0.29 475 166 170 4 787.71 0.89 476 166 188 22 727.46 1.75 477 166 189 23 688.25 0.29 478 166 192 26 641.83 7.00 479 166 193 27 479.56 61.49 480 167 170 3 792.62 1.57 481 167 171 4 782.54 1.68 482 168 171 3 800.43 5.04 483 168 172 4 775.48 1.03 484 169 172 3 807.88 2.63 485 169 173 4 775.46 1.04 486 169 182 13 730.84 2.02 487 169 193 24 448.67 51.52 488 170 173 3 798.89 7.20 489 170 174 4 792.89 10.20 490 170 192 22 575.66 13.43 491 170 193 23 368.71 91.50 492 171 174 3 802.73 5.28 493 171 175 4 790.44 11.42 494 172 175 3 802.73 5.28 495 172 176 4 790.45 11.42 496 173 176 3 811.96 0.66 497 173 177 4 763.15 1.15 498 173 178 5 743.06 7.96 499 173 180 7 730.93 2.00 500 174 177 3 780.84 2.12 501 182 189 7 730.90 2.06 502 182 193 11 172.42 3.29 503 184 189 5 779.80 3.09 504 186 189 3 791.30 4.69 505 186 190 4 783.97 1.03 506 187 190 3 786.37 2.23 507 187 191 4 781.61 2.79 508 187 225 38 61.34 0.21 509 187 226 39 61.30 0.19 510 187 228 41 57.34 0.78 511 187 229 42 54.77 0.51 512 187 232 45 41.23 0.64 513 188 191 3 797.76 0.91 514 188 192 4 781.62 2.79 515 188 232 44 38.92 0.52 516 189 192 3 797.07 0.59 517 189 193 4 781.65 2.77 518 190 193 3 807.30 6.84 519 190 194 4 764.25 11.45 520 190 197 7 170.13 2.40 521 190 211 21 132.50 9.97 522 191 211 20 133.03 10.24 523 191 229 38 54.73 0.49 524 194 197 3 817.96 1.53 525 195 210 15 140.88 14.29 526 196 208 12 142.78 15.09 527 196 209 13 142.69 15.08 528 197 208 11 142.76 15.08 529 197 209 12 142.71 15.06 530 197 211 14 131.35 10.45 531 197 214 17 112.20 8.88 532 198 205 7 146.03 13.57 533 198 207 9 144.75 14.12 534 198 208 10 142.72 15.06 535 199 205 6 146.00 13.55 536 199 206 7 145.80 13.60 537 199 207 8 145.73 13.62 538 199 214 15 111.06 8.29 539 199 222 23 61.34 0.21 540 199 225 26 61.30 0.19 541 200 203 3 818.82 0.42 542 200 204 4 579.15 5.12 543 200 205 5 145.93 13.52 544 201 204 3 815.72 0.13 545 201 206 5 449.75 53.20 546 201 221 20 61.38 0.21 547 206 221 15 61.36 0.21 548 206 222 16 61.33 0.20 549 207 214 7 571.42 7.08 550 207 219 12 61.41 0.20 551 207 222 15 61.30 0.18 552 208 214 6 753.49 8.98 553 210 213 3 800.46 0.98 554 210 214 4 785.97 9.53 555 211 214 3 792.19 6.41 556 211 215 4 785.97 9.53 557 211 226 15 61.24 0.19 558 211 229 18 57.30 0.79 559 212 215 3 806.26 0.61 560 212 216 4 792.88 6.07 561 213 216 3 832.72 0.51 562 213 217 4 770.93 2.02 563 214 226 12 61.25 0.20 564 215 218 3 797.88 3.55 565 215 226 11 61.30 0.21 566 215 229 14 60.57 0.38 567 218 223 5 557.67 8.65 568 219 223 4 772.12 2.81 569 220 223 3 775.35 1.22 570 220 224 4 772.12 2.81 571 221 224 3 789.08 0.21 572 221 225 4 777.49 0.16 573 222 225 3 792.87 4.46 574 222 226 4 775.36 1.22 575 223 226 3 802.76 5.73 576 223 227 4 796.54 2.62 577 224 227 3 811.55 2.58 578 225 228 3 852.21 1.24 579 226 229 3 843.02 5.83 580 227 230 3 834.36 1.55 581 228 231 3 817.43 1.31 582 228 232 4 791.40 5.49 583 229 232 3 805.99 1.80 584 229 233 4 782.45 2.93 585 230 233 3 792.01 0.94 586 230 234 4 785.26 4.33 587 231 234 3 789.59 4.83 588 231 235 4 789.64 2.15 589 232 235 3 800.82 2.77 590 232 236 4 789.70 2.12 591 233 236 3 798.31 3.18 592 233 237 4 789.71 2.12 593 234 237 3 802.08 6.62 594 234 238 4 786.83 0.68 595 235 238 3 795.03 4.79 596 235 239 4 786.80 0.67 597 236 239 3 790.61 1.22