BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 4 200 196 186.11 0.49 2 4 201 197 186.10 0.48 3 5 38 33 464.56 1.54 4 5 39 34 464.55 1.54 5 5 199 194 186.19 0.47 6 5 201 196 186.09 0.49 7 6 39 33 464.55 1.54 8 6 40 34 464.55 1.54 9 6 41 35 464.55 1.54 10 6 65 59 464.54 1.54 11 6 195 189 186.41 0.50 12 6 199 193 186.28 0.48 13 6 201 195 186.16 0.49 14 6 202 196 186.10 0.48 15 6 203 197 186.10 0.48 16 7 33 26 464.81 1.49 17 7 38 31 464.76 1.49 18 7 39 32 464.63 1.53 19 7 40 33 464.57 1.54 20 7 41 34 464.57 1.54 21 7 203 196 186.12 0.48 22 7 205 198 186.10 0.48 23 7 239 232 45.54 0.03 24 7 242 235 34.70 0.30 25 8 41 33 464.58 1.54 26 8 67 59 464.54 1.53 27 8 191 183 187.69 0.33 28 8 192 184 187.63 0.34 29 8 195 187 187.38 0.33 30 8 202 194 186.25 0.48 31 8 203 195 186.19 0.48 32 8 204 196 186.12 0.48 33 8 205 197 186.12 0.48 34 8 218 210 112.20 1.96 35 9 14 5 762.16 1.03 36 9 15 6 734.26 14.94 37 9 30 21 465.34 1.48 38 9 40 31 464.80 1.50 39 9 41 32 464.75 1.49 40 9 42 33 464.62 1.52 41 9 43 34 464.62 1.52 42 9 205 196 186.12 0.48 43 9 235 226 62.73 0.57 44 9 239 230 46.16 0.05 45 10 14 4 762.28 1.09 46 10 15 5 762.28 1.09 47 10 43 33 464.62 1.52 48 10 188 178 189.05 0.12 49 10 204 194 186.26 0.48 50 10 205 195 186.19 0.48 51 10 206 196 186.12 0.48 52 10 207 197 186.12 0.48 53 10 215 205 113.35 1.64 54 11 14 3 828.53 3.40 55 11 15 4 762.28 1.09 56 11 16 5 762.28 1.09 57 11 42 31 464.79 1.50 58 11 51 40 464.67 1.53 59 11 54 43 465.06 1.47 60 11 55 44 465.19 1.52 61 12 16 4 770.90 5.41 62 12 18 6 619.03 72.50 63 12 19 7 617.40 73.31 64 12 54 42 465.35 1.44 65 12 207 195 186.19 0.48 66 13 16 3 840.25 1.33 67 13 18 5 618.96 72.53 68 13 207 194 186.18 0.48 69 13 231 218 87.73 1.00 70 13 232 219 83.69 1.05 71 14 205 191 186.21 0.48 72 14 206 192 186.16 0.48 73 14 207 193 186.12 0.48 74 14 229 215 96.31 0.65 75 14 232 218 77.31 0.31 76 14 235 221 67.61 0.38 77 15 22 7 466.49 1.57 78 15 26 11 466.24 1.65 79 15 30 15 466.19 1.63 80 15 42 27 464.79 1.50 81 15 55 40 466.03 1.66 82 15 63 48 466.05 1.65 83 15 232 217 75.22 0.73 84 15 235 220 67.66 0.37 85 16 20 4 801.37 6.24 86 16 21 5 587.25 59.80 87 16 22 6 466.49 1.56 88 16 54 38 466.06 1.66 89 16 55 39 466.07 1.67 90 16 232 216 77.31 0.31 91 17 20 3 828.31 3.50 92 17 21 4 820.25 0.45 93 17 23 6 469.14 0.74 94 17 232 215 83.70 1.05 95 21 54 33 466.07 1.67 96 22 26 4 824.23 0.98 97 22 27 5 824.24 0.98 98 22 30 8 800.60 1.13 99 22 54 32 466.09 1.67 100 22 55 33 466.09 1.67 101 23 26 3 835.33 4.20 102 23 27 4 825.08 0.58 103 23 232 209 83.64 1.03 104 24 27 3 825.08 0.58 105 24 28 4 825.49 0.79 106 25 28 3 831.93 1.48 107 25 29 4 825.75 0.92 108 25 232 207 83.62 1.03 109 25 236 211 67.64 0.37 110 26 29 3 832.71 3.04 111 26 30 4 825.81 0.95 112 26 232 206 77.28 0.29 113 26 235 209 68.09 0.38 114 26 236 210 67.36 0.51 115 26 239 213 58.68 0.25 116 27 30 3 841.04 1.13 117 27 31 4 827.47 0.11 118 27 55 28 466.23 1.69 119 28 31 3 834.58 0.24 120 28 32 4 827.34 0.04 121 29 32 3 860.18 7.12 122 29 33 4 827.34 0.04 123 29 236 207 57.41 2.10 124 29 239 210 47.79 0.61 125 30 33 3 827.35 0.04 126 30 34 4 834.83 3.71 127 30 40 10 484.28 4.07 128 30 63 33 475.33 2.34 129 30 239 209 46.52 0.02 130 31 34 3 842.43 0.45 131 31 35 4 831.50 2.04 132 32 35 3 837.77 4.07 133 32 36 4 834.48 3.53 134 32 243 211 34.73 0.29 135 33 36 3 835.59 2.97 136 33 37 4 832.50 4.51 137 33 38 5 796.11 0.76 138 33 40 7 762.09 4.86 139 33 239 206 46.09 0.08 140 33 243 210 34.69 0.30 141 34 37 3 833.76 6.08 142 34 38 4 805.46 4.04 143 34 40 6 773.53 9.52 144 34 61 27 522.51 3.58 145 34 62 28 513.63 0.85 146 34 63 29 513.63 0.85 147 34 64 30 516.82 0.74 148 36 243 207 34.70 0.30 149 38 242 204 34.70 0.29 150 38 243 205 34.38 0.46 151 39 64 25 573.04 0.64 152 39 199 160 186.29 0.48 153 40 62 22 573.13 0.67 154 40 63 23 573.10 0.67 155 40 64 24 573.11 0.67 156 40 65 25 573.06 0.64 157 41 63 22 573.12 0.67 158 41 65 24 573.06 0.64 159 42 51 9 573.28 0.70 160 42 55 13 573.36 0.68 161 42 63 21 573.25 0.67 162 42 65 23 573.17 0.67 163 42 66 24 573.06 0.64 164 42 67 25 573.02 0.66 165 43 67 24 573.02 0.66 166 43 70 27 548.93 2.48 167 43 188 145 188.87 0.21 168 43 191 148 188.11 0.47 169 43 204 161 186.25 0.49 170 43 218 175 112.45 1.83 171 44 51 7 660.49 42.88 172 44 67 23 573.16 0.68 173 44 68 24 573.06 0.65 174 44 69 25 573.01 0.65 175 44 70 26 572.46 0.84 176 44 188 144 189.18 0.14 177 45 69 24 573.01 0.65 178 45 70 25 572.98 0.67 179 45 188 143 189.27 0.13 180 46 50 4 818.57 2.28 181 46 51 5 814.13 4.49 182 46 68 22 573.25 0.66 183 46 69 23 573.11 0.68 184 47 50 3 846.68 1.17 185 47 51 4 827.80 0.14 186 47 68 21 573.26 0.65 187 47 69 22 573.19 0.65 188 48 51 3 831.51 2.00 189 48 52 4 827.68 0.08 190 48 59 11 573.89 0.63 191 48 60 12 573.88 0.62 192 48 66 18 573.37 0.67 193 48 68 20 573.28 0.66 194 48 73 25 500.07 7.13 195 48 75 27 492.19 10.95 196 49 52 3 827.69 0.08 197 49 53 4 821.46 0.85 198 50 53 3 832.97 2.55 199 50 54 4 821.46 0.85 200 51 54 3 840.62 1.37 201 51 55 4 817.32 2.36 202 51 59 8 573.88 0.63 203 51 66 15 573.37 0.65 204 51 67 16 573.35 0.64 205 51 68 17 573.27 0.64 206 52 55 3 829.45 0.79 207 52 56 4 806.69 6.49 208 52 57 5 748.52 1.58 209 52 59 7 573.87 0.64 210 55 59 4 794.74 12.81 211 55 63 8 573.72 0.62 212 56 59 3 842.06 2.91 213 58 61 3 839.95 0.40 214 58 62 4 817.05 3.33 215 59 62 3 830.94 3.95 216 59 63 4 801.46 4.45 217 59 66 7 573.48 0.69 218 60 63 3 857.50 0.07 219 60 66 6 573.48 0.69 220 60 75 15 341.75 18.21 221 60 78 18 341.44 18.19 222 63 66 3 879.39 0.58 223 64 76 12 341.50 18.22 224 64 77 13 341.44 18.20 225 65 75 10 341.73 18.22 226 65 76 11 341.49 18.23 227 65 195 130 186.40 0.51 228 65 199 134 186.41 0.49 229 66 74 8 341.87 18.27 230 66 75 9 341.73 18.21 231 67 70 3 842.66 0.66 232 67 71 4 543.15 14.50 233 67 73 6 430.93 19.71 234 67 74 7 341.88 18.26 235 67 81 14 341.43 18.20 236 67 191 124 187.53 0.39 237 67 194 127 187.55 0.33 238 67 195 128 187.48 0.36 239 68 71 3 823.84 0.29 240 68 72 4 793.31 2.14 241 68 73 5 469.53 0.41 242 69 72 3 837.76 0.69 243 69 123 54 216.24 2.29 244 69 124 55 209.72 1.69 245 70 186 116 189.56 0.01 246 70 187 117 189.22 0.15 247 70 188 118 189.09 0.16 248 70 190 120 188.84 0.28 249 70 191 121 188.69 0.27 250 71 74 3 812.01 3.35 251 71 82 11 341.26 18.27 252 71 190 119 188.69 0.28 253 71 191 120 187.52 0.39 254 72 83 11 340.51 17.91 255 72 84 12 340.02 18.06 256 72 124 52 215.00 1.68 257 72 186 114 190.00 0.16 258 72 190 118 188.92 0.26 259 73 80 7 341.56 18.19 260 73 82 9 341.36 18.23 261 73 83 10 340.56 18.07 262 74 80 6 341.49 18.18 263 74 81 7 341.40 18.21 264 74 82 8 341.19 18.30 265 75 79 4 563.95 93.01 266 75 80 5 341.43 18.20 267 75 81 6 341.37 18.22 268 76 79 3 829.18 0.73 269 76 81 5 563.89 93.04 270 80 83 3 948.41 1.24 271 81 194 113 186.40 0.51 272 82 190 108 188.76 0.33 273 82 194 112 186.42 0.49 274 83 88 5 841.81 7.80 275 84 88 4 841.28 8.06 276 84 123 39 248.28 0.34 277 84 186 102 195.43 1.81 278 84 190 106 189.68 0.09 279 85 88 3 849.49 3.97 280 85 89 4 814.35 5.41 281 85 123 38 248.19 0.29 282 85 186 101 203.33 1.43 283 86 89 3 841.81 7.79 284 86 90 4 807.38 8.89 285 86 121 35 251.91 2.06 286 86 122 36 251.87 2.07 287 86 123 37 251.64 2.00 288 86 124 38 248.30 0.45 289 86 125 39 248.07 0.34 290 86 126 40 251.68 2.04 291 86 183 97 228.83 1.33 292 86 186 100 218.59 4.00 293 87 90 3 837.00 5.55 294 87 91 4 801.20 8.62 295 87 92 5 801.20 8.62 296 87 95 8 766.48 1.74 297 87 121 34 254.86 0.69 298 89 183 94 229.06 1.43 299 89 186 97 224.18 2.04 300 90 94 4 808.13 8.45 301 90 95 5 808.16 8.44 302 90 126 36 259.32 0.20 303 90 167 77 248.39 0.42 304 91 94 3 852.49 3.37 305 91 95 4 821.33 1.87 306 92 95 3 828.11 1.52 307 92 96 4 821.33 1.86 308 93 96 3 843.00 1.87 309 93 97 4 821.32 1.86 310 94 97 3 832.01 3.48 311 94 98 4 821.30 1.86 312 94 166 72 332.24 11.90 313 94 167 73 328.94 13.32 314 95 98 3 850.57 1.26 315 95 99 4 819.43 1.66 316 95 121 26 336.35 12.23 317 95 126 31 333.60 10.92 318 96 99 3 844.49 1.78 319 96 100 4 822.77 0.01 320 97 100 3 835.41 6.32 321 97 101 4 823.64 0.45 322 97 164 67 337.30 12.13 323 97 167 70 337.18 12.15 324 98 101 3 844.63 1.11 325 98 102 4 831.61 2.91 326 98 126 28 337.19 12.16 327 98 160 62 337.27 12.14 328 98 164 66 337.40 12.10 329 98 167 69 337.19 12.15 330 98 169 71 337.16 12.17 331 98 171 73 337.22 12.17 332 98 181 83 337.14 12.14 333 99 102 3 833.81 1.81 334 99 103 4 811.71 1.95 335 99 119 20 337.53 12.19 336 99 126 27 337.23 12.17 337 100 103 3 836.13 3.17 338 100 104 4 828.81 4.30 339 101 104 3 841.42 0.52 340 101 105 4 828.82 4.30 341 101 160 59 340.16 10.90 342 101 163 62 340.17 10.89 343 101 164 63 340.20 10.88 344 102 105 3 842.05 0.21 345 102 106 4 816.47 4.32 346 102 119 17 340.66 10.64 347 102 128 26 340.44 10.76 348 102 160 58 340.16 10.90 349 103 106 3 842.88 0.62 350 103 107 4 813.97 3.12 351 103 119 16 340.67 10.64 352 104 107 3 826.34 2.94 353 104 108 4 813.33 2.78 354 105 108 3 838.60 6.54 355 105 109 4 831.49 2.99 356 105 156 51 341.05 10.74 357 105 159 54 341.08 10.73 358 105 160 55 340.97 10.79 359 106 109 3 831.88 3.18 360 106 114 8 492.56 2.51 361 106 118 12 341.20 10.69 362 106 119 13 341.19 10.70 363 106 128 22 341.18 10.70 364 106 129 23 341.19 10.70 365 106 130 24 341.18 10.70 366 106 156 50 341.16 10.71 367 109 114 5 819.19 1.52 368 109 130 21 341.25 10.70 369 109 152 43 592.12 4.12 370 109 156 47 341.24 10.70 371 110 150 40 639.46 1.33 372 111 114 3 929.51 6.33 373 111 130 19 631.81 2.46 374 111 131 20 638.54 0.84 375 111 143 32 643.73 0.51 376 111 146 35 643.74 0.51 377 111 150 39 639.83 1.43 378 111 152 41 637.77 0.51 379 112 146 34 643.75 0.51 380 112 150 38 643.75 0.51 381 113 131 18 656.63 5.88 382 113 139 26 663.72 2.28 383 114 118 4 882.75 3.31 384 114 130 16 656.66 5.89 385 114 131 17 656.63 5.88 386 115 118 3 1026.89 5.97 387 115 131 16 668.72 0.14 388 115 134 19 668.78 0.30 389 115 135 20 668.65 0.33 390 115 139 24 668.58 0.29 391 116 132 16 668.89 0.21 392 116 133 17 668.95 0.21 393 116 134 18 668.79 0.29 394 117 120 3 875.92 0.39 395 117 129 12 669.00 0.21 396 117 132 15 668.97 0.23 397 117 133 16 668.97 0.23 398 117 134 17 668.96 0.24 399 118 128 10 669.03 0.21 400 118 129 11 668.98 0.20 401 118 130 12 668.98 0.19 402 118 131 13 669.00 0.21 403 118 132 14 668.98 0.23 404 119 127 8 669.16 0.21 405 119 128 9 669.08 0.24 406 120 126 6 669.22 0.21 407 120 127 7 669.15 0.23 408 120 133 13 669.08 0.25 409 121 126 5 669.22 0.21 410 122 125 3 831.55 3.18 411 122 127 5 674.21 2.27 412 124 182 58 668.25 0.46 413 124 186 62 622.56 0.49 414 125 180 55 668.55 0.38 415 125 181 56 668.33 0.42 416 125 182 57 668.13 0.46 417 126 180 54 668.35 0.42 418 126 181 55 668.28 0.40 419 127 133 6 779.47 2.04 420 127 178 51 668.62 0.36 421 127 179 52 668.50 0.34 422 127 180 53 668.18 0.45 423 128 178 50 668.60 0.35 424 128 179 51 668.47 0.35 425 128 181 53 668.12 0.46 426 129 132 3 797.63 5.41 427 129 133 4 806.47 6.01 428 129 177 48 668.70 0.37 429 129 178 49 668.50 0.35 430 130 143 13 669.59 0.67 431 130 152 22 669.17 0.53 432 130 153 23 669.07 0.51 433 130 156 26 669.02 0.52 434 130 177 47 669.05 0.50 435 131 135 4 835.08 0.62 436 131 139 8 702.68 11.52 437 131 143 12 669.57 0.68 438 131 177 46 669.16 0.54 439 132 135 3 861.36 1.16 440 132 177 45 669.38 0.67 441 133 140 7 766.47 7.74 442 133 177 44 669.33 0.73 443 133 178 45 669.31 0.73 444 135 139 4 816.91 4.27 445 135 140 5 810.65 1.16 446 135 177 42 669.64 0.80 447 136 139 3 820.57 2.50 448 136 140 4 816.97 4.30 449 137 140 3 827.61 4.78 450 137 141 4 822.40 1.58 451 137 176 39 670.58 0.39 452 138 141 3 831.56 0.53 453 138 142 4 822.40 1.58 454 139 142 3 841.03 4.75 455 139 143 4 822.38 1.57 456 140 143 3 843.03 3.75 457 140 144 4 837.98 6.28 458 140 176 36 671.10 0.12 459 140 177 37 671.10 0.12 460 141 144 3 846.49 2.02 461 141 145 4 843.06 3.73 462 141 176 35 671.38 0.03 463 142 145 3 842.99 3.77 464 142 146 4 815.03 1.25 465 143 146 3 829.21 5.45 466 143 147 4 810.28 1.12 467 143 152 9 747.04 7.33 468 143 153 10 746.48 7.05 469 143 176 33 671.50 0.01 470 143 177 34 671.50 0.01 471 144 147 3 842.48 1.12 472 144 148 4 833.56 3.27 473 144 176 32 671.45 0.01 474 145 148 3 854.15 4.71 475 145 149 4 839.85 0.13 476 146 149 3 855.18 4.21 477 146 150 4 837.58 1.00 478 146 152 6 808.22 0.41 479 147 150 3 851.60 6.00 480 147 151 4 816.65 3.85 481 147 152 5 808.16 0.39 482 147 153 6 808.15 0.38 483 148 151 3 839.51 0.05 484 150 155 5 831.31 2.68 485 151 154 3 840.37 1.79 486 151 155 4 834.94 4.50 487 152 155 3 848.03 1.29 488 152 156 4 841.06 2.19 489 153 156 3 847.81 1.18 490 153 157 4 841.06 2.19 491 153 173 20 677.67 0.29 492 153 174 21 678.50 0.26 493 153 175 22 678.58 0.27 494 153 176 23 678.48 0.29 495 153 177 24 678.46 0.28 496 153 178 25 678.25 0.20 497 153 179 26 677.71 0.27 498 154 157 3 841.06 2.19 499 154 158 4 841.05 2.19 500 155 158 3 841.90 1.77 501 155 159 4 822.03 0.77 502 156 159 3 837.88 0.62 503 156 160 4 820.07 1.75 504 156 179 23 678.43 0.26 505 157 160 3 834.94 0.94 506 157 161 4 825.87 3.80 507 157 171 14 677.81 0.33 508 157 173 16 678.59 0.23 509 157 179 22 678.48 0.29 510 158 161 3 836.71 1.82 511 158 162 4 825.34 3.54 512 159 162 3 842.51 4.75 513 159 163 4 825.67 3.37 514 160 163 3 841.99 4.71 515 160 164 4 825.66 3.36 516 160 171 11 678.59 0.23 517 160 179 19 678.71 0.28 518 161 165 4 789.78 13.92 519 161 171 10 678.59 0.23 520 162 165 3 851.24 1.74 521 164 167 3 846.81 3.96 522 165 168 3 848.40 1.03 523 168 183 15 678.65 0.27 524 168 184 16 678.52 0.23 525 169 181 12 678.87 0.29 526 169 182 13 678.83 0.29 527 169 183 14 678.64 0.27 528 169 184 15 678.62 0.28 529 170 181 11 678.90 0.29 530 170 182 12 678.86 0.29 531 170 184 14 678.55 0.26 532 171 179 8 679.08 0.28 533 171 180 9 679.03 0.26 534 171 181 10 678.91 0.29 535 172 179 7 679.08 0.28 536 172 180 8 679.03 0.26 537 172 182 10 679.01 0.28 538 173 178 5 679.24 0.28 539 173 179 6 679.12 0.27 540 174 178 4 679.24 0.28 541 174 179 5 679.19 0.30 542 182 186 4 803.28 3.41 543 183 186 3 838.52 2.20 544 185 190 5 443.56 117.63 545 186 190 4 723.03 10.61 546 187 190 3 874.06 2.70 547 187 217 30 113.39 1.48 548 188 191 3 874.06 2.70 549 188 204 16 186.33 0.49 550 188 214 26 114.75 1.86 551 188 215 27 113.59 1.55 552 188 218 30 112.34 1.90 553 189 192 3 845.64 0.58 554 189 217 28 112.25 1.95 555 189 218 29 112.20 1.96 556 189 221 32 109.98 1.41 557 189 223 34 102.86 1.82 558 190 193 3 876.77 2.27 559 190 194 4 865.95 2.04 560 191 194 3 850.63 1.56 561 191 195 4 843.06 2.21 562 191 218 27 112.20 1.96 563 192 195 3 850.63 1.56 564 192 196 4 826.12 0.99 565 192 218 26 112.20 1.96 566 192 221 29 107.97 1.40 567 192 223 31 97.86 0.61 568 193 196 3 847.05 1.39 569 195 198 3 833.75 0.97 570 195 199 4 750.12 5.46 571 195 202 7 192.68 3.72 572 196 202 6 460.15 127.47 573 196 223 27 97.84 0.61 574 199 202 3 878.86 5.12 575 201 225 24 96.37 0.66 576 202 223 21 97.83 0.62 577 202 224 22 96.81 0.61 578 202 225 23 96.38 0.66 579 203 225 22 96.37 0.67 580 203 227 24 96.36 0.67 581 203 242 39 34.38 0.46 582 204 215 11 113.49 1.50 583 204 218 14 112.19 1.95 584 204 219 15 111.70 2.03 585 204 224 20 97.88 0.61 586 204 225 21 97.79 0.64 587 204 226 22 96.53 0.61 588 204 227 23 96.36 0.67 589 205 227 22 96.37 0.67 590 205 235 30 57.97 0.62 591 205 238 33 46.52 0.12 592 205 239 34 46.15 0.06 593 206 209 3 859.27 7.52 594 206 215 9 114.42 1.94 595 206 227 21 97.62 0.71 596 206 228 22 96.53 0.61 597 206 229 23 96.34 0.67 598 207 215 8 115.22 2.00 599 207 229 22 96.37 0.66 600 208 213 5 115.27 2.00 601 208 214 6 115.27 2.00 602 208 215 7 115.22 2.00 603 208 216 8 115.22 2.00 604 209 215 6 115.25 2.00 605 209 216 7 115.20 2.00 606 209 219 10 114.73 1.90 607 209 228 19 97.85 0.62 608 210 213 3 816.66 3.66 609 210 216 6 115.25 1.99 610 211 216 5 155.38 22.04 611 213 216 3 822.00 6.74 612 215 218 3 845.64 2.54 613 215 219 4 809.34 2.99 614 216 219 3 838.91 0.83 615 216 220 4 812.32 1.51 616 217 220 3 843.54 3.60 617 217 221 4 806.98 4.17 618 218 221 3 816.22 3.46 619 218 224 6 449.52 115.28 620 219 224 5 217.06 3.70 621 219 226 7 97.87 0.61 622 219 227 8 97.88 0.61 623 219 228 9 97.84 0.61 624 221 224 3 840.09 3.40 625 227 238 11 46.34 0.12 626 229 234 5 614.00 60.35 627 229 235 6 709.93 11.61 628 230 234 4 812.93 1.03 629 231 234 3 837.44 3.33 630 231 235 4 830.06 0.35 631 232 235 3 830.06 0.35 632 232 236 4 830.07 0.34 633 233 236 3 835.85 1.03 634 233 237 4 830.08 0.35 635 234 237 3 830.08 0.35 636 234 238 4 821.88 3.75 637 235 238 3 837.25 3.26 638 235 239 4 820.65 3.14 639 236 239 3 830.78 0.03 640 236 240 4 807.85 3.25 641 237 240 3 831.92 8.82 642 237 241 4 816.75 1.24 643 238 241 3 825.45 1.91 644 238 242 4 807.58 3.13 645 239 242 3 807.96 2.94 646 239 243 4 807.54 3.15 647 240 243 3 822.77 4.48 648 240 244 4 807.52 3.14 649 241 244 3 828.59 3.79 650 241 245 4 822.73 4.50 651 242 245 3 847.88 6.83