BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 40 76 36 263.43 0.60 2 42 50 8 681.78 0.99 3 43 50 7 681.78 0.99 4 43 52 9 681.59 1.03 5 43 71 28 264.56 0.49 6 43 77 34 264.45 0.48 7 43 78 35 264.31 0.53 8 44 50 6 681.78 0.99 9 44 52 8 681.76 0.99 10 44 78 34 264.92 0.49 11 44 80 36 265.26 0.40 12 44 86 42 265.22 0.42 13 44 140 96 359.46 46.69 14 44 141 97 265.28 0.40 15 44 142 98 265.28 0.40 16 45 48 3 830.73 1.42 17 45 50 5 714.20 12.72 18 45 141 96 265.39 0.42 19 45 142 97 265.43 0.41 20 46 141 95 391.95 30.37 21 46 142 96 265.44 0.41 22 46 143 97 265.44 0.41 23 46 144 98 265.44 0.41 24 47 141 94 432.20 12.17 25 48 141 93 434.19 11.94 26 49 87 38 609.29 4.38 27 49 88 39 609.16 4.28 28 49 89 40 621.18 10.30 29 49 98 49 628.70 7.77 30 49 139 90 438.65 10.43 31 49 140 91 435.06 12.21 32 49 141 92 434.25 11.97 33 50 72 22 664.24 1.71 34 50 139 89 483.09 1.24 35 50 140 90 459.56 12.72 36 51 72 21 664.46 1.80 37 51 137 86 520.72 3.12 38 51 138 87 512.09 5.55 39 51 139 88 506.58 2.98 40 52 69 17 663.38 1.52 41 52 70 18 664.12 1.75 42 52 71 19 664.12 1.78 43 52 72 20 664.49 1.82 44 52 113 61 663.61 1.56 45 52 137 85 522.72 3.99 46 52 138 86 517.76 4.59 47 52 140 88 515.88 3.66 48 53 69 16 664.44 1.91 49 53 70 17 664.24 1.72 50 53 135 82 527.08 4.39 51 53 136 83 525.99 4.33 52 53 137 84 523.13 4.16 53 54 57 3 951.30 2.43 54 54 67 13 664.45 1.92 55 54 68 14 664.45 1.91 56 54 69 15 664.53 1.86 57 54 132 78 527.20 4.38 58 54 133 79 527.23 4.38 59 54 134 80 527.22 4.38 60 54 135 81 527.19 4.38 61 54 136 82 527.08 4.39 62 55 68 13 664.61 1.84 63 55 69 14 664.60 1.83 64 55 70 15 664.65 1.82 65 55 111 56 664.73 1.80 66 55 112 57 664.66 1.79 67 55 134 79 527.21 4.38 68 56 67 11 664.66 1.86 69 56 68 12 664.63 1.85 70 56 134 78 527.45 4.42 71 57 66 9 670.27 0.83 72 57 67 10 664.65 1.86 73 57 130 73 528.29 4.48 74 58 65 7 680.74 1.70 75 58 66 8 670.36 0.84 76 59 64 5 733.11 19.20 77 59 65 6 682.41 2.54 78 60 64 4 783.80 6.16 79 60 65 5 708.45 8.83 80 60 66 6 698.36 5.36 81 60 116 56 664.65 1.77 82 60 199 139 324.32 1.84 83 61 64 3 822.58 1.67 84 61 65 4 810.58 7.24 85 61 196 135 324.69 1.69 86 61 197 136 324.69 1.71 87 61 199 138 324.55 1.76 88 62 197 135 324.72 1.71 89 63 118 55 664.30 1.68 90 63 119 56 664.27 1.68 91 64 116 52 664.74 1.73 92 64 117 53 664.50 1.65 93 64 118 54 664.27 1.69 94 64 199 135 324.26 1.86 95 65 116 51 664.68 1.70 96 65 117 52 664.52 1.63 97 65 119 54 664.38 1.61 98 65 130 65 533.91 2.04 99 66 104 38 664.98 1.64 100 66 114 48 664.95 1.63 101 66 115 49 664.85 1.61 102 66 116 50 664.51 1.59 103 67 114 47 664.91 1.61 104 67 115 48 664.78 1.56 105 67 117 50 664.53 1.56 106 67 129 62 528.06 4.37 107 67 130 63 528.23 4.43 108 68 112 44 665.65 1.49 109 68 113 45 665.21 1.65 110 68 114 46 664.91 1.60 111 69 112 43 665.67 1.50 112 69 113 44 665.60 1.49 113 69 115 46 665.21 1.67 114 69 132 63 527.46 4.30 115 69 138 69 526.29 4.19 116 70 111 41 665.89 1.56 117 70 112 42 665.73 1.52 118 71 76 5 684.75 0.07 119 71 77 6 680.06 2.42 120 71 78 7 676.45 1.77 121 71 111 40 666.35 1.79 122 71 112 41 665.87 1.58 123 71 113 42 665.87 1.57 124 72 76 4 805.15 15.70 125 73 76 3 814.02 11.26 126 73 111 38 667.88 1.83 127 74 110 36 669.58 2.46 128 74 111 37 667.92 1.81 129 75 110 35 669.60 2.48 130 76 110 34 669.84 2.44 131 76 111 35 669.69 2.49 132 77 107 30 669.95 2.44 133 77 108 31 669.94 2.44 134 77 109 32 669.90 2.45 135 77 110 33 669.87 2.44 136 78 86 8 681.69 0.07 137 78 103 25 669.82 2.48 138 78 113 35 669.73 2.48 139 80 84 4 753.08 2.14 140 80 85 5 706.26 5.48 141 80 86 6 682.50 0.10 142 80 103 23 669.99 2.44 143 80 143 63 523.90 3.81 144 80 171 91 442.38 0.20 145 81 84 3 841.11 5.79 146 81 143 62 524.54 3.62 147 81 171 90 442.41 0.21 148 82 147 65 522.59 4.00 149 82 149 67 493.47 3.08 150 82 171 89 442.40 0.21 151 82 172 90 441.88 0.03 152 82 175 93 438.25 0.33 153 83 147 64 521.66 3.54 154 83 171 88 442.41 0.21 155 83 172 89 441.81 0.01 156 83 281 198 15.19 0.79 157 84 143 59 524.83 3.52 158 84 144 60 524.45 3.65 159 84 145 61 523.88 3.73 160 84 147 63 521.74 3.50 161 84 171 87 442.40 0.21 162 85 100 15 673.25 3.06 163 85 101 16 673.08 3.08 164 85 102 17 672.39 2.83 165 85 143 58 524.80 3.51 166 85 144 59 524.22 3.77 167 85 145 60 523.88 3.73 168 86 100 14 673.27 3.08 169 86 101 15 673.12 3.10 170 86 103 17 673.08 3.08 171 86 113 27 671.51 2.62 172 86 115 29 671.40 2.59 173 86 140 54 527.59 4.47 174 86 141 55 527.19 4.34 175 87 99 12 673.61 2.93 176 87 100 13 673.58 2.93 177 87 140 53 527.62 4.49 178 87 141 54 527.52 4.50 179 87 144 57 525.40 3.81 180 88 98 10 673.69 2.96 181 88 99 11 673.65 2.95 182 88 101 13 673.61 2.94 183 88 125 37 577.64 12.54 184 88 129 41 574.43 11.09 185 88 138 50 527.96 4.48 186 88 139 51 527.84 4.51 187 88 140 52 527.62 4.49 188 89 96 7 681.76 0.04 189 89 97 8 674.99 3.39 190 89 98 9 673.73 2.94 191 89 125 36 577.71 12.56 192 89 139 50 527.96 4.48 193 90 96 6 681.89 0.02 194 90 97 7 681.72 0.06 195 90 125 35 577.75 12.49 196 90 128 38 577.39 12.44 197 91 94 3 834.13 1.97 198 91 95 4 754.37 16.13 199 91 96 5 683.78 0.91 200 91 128 37 577.66 12.45 201 92 95 3 825.91 2.00 202 92 97 5 701.20 7.71 203 92 124 32 656.59 2.40 204 92 128 36 577.70 12.44 205 97 124 27 577.84 12.50 206 97 125 28 577.79 12.52 207 98 125 27 577.71 12.56 208 99 124 25 577.81 12.51 209 99 125 26 576.83 12.15 210 101 115 14 671.45 2.62 211 101 117 16 670.87 2.69 212 101 125 24 576.89 12.19 213 101 129 28 564.06 6.28 214 102 115 13 671.46 2.62 215 102 167 65 441.82 0.00 216 102 168 66 441.42 0.20 217 102 171 69 441.39 0.21 218 103 113 10 671.59 2.66 219 103 114 11 671.50 2.64 220 103 115 12 671.46 2.62 221 104 114 10 671.58 2.67 222 104 116 12 671.48 2.62 223 104 167 63 441.84 0.03 224 104 174 70 438.23 0.20 225 104 200 96 323.97 1.88 226 105 109 4 774.53 16.25 227 105 112 7 672.91 2.04 228 105 113 8 671.62 2.68 229 105 114 9 671.57 2.66 230 106 109 3 833.08 6.29 231 106 174 68 434.12 1.72 232 109 112 3 874.10 2.62 233 115 138 23 527.67 4.48 234 116 167 51 441.41 0.18 235 116 199 83 324.25 1.86 236 117 126 9 567.32 7.87 237 117 129 12 549.33 1.05 238 118 126 8 674.25 3.43 239 118 163 45 441.40 0.18 240 118 164 46 441.40 0.18 241 119 126 7 674.26 3.43 242 119 127 8 674.26 3.42 243 119 130 11 549.76 0.84 244 120 123 3 804.22 5.82 245 120 126 6 683.89 1.37 246 120 127 7 704.88 9.12 247 121 127 6 766.64 6.45 248 123 126 3 826.49 2.35 249 123 127 4 804.72 2.47 250 124 127 3 823.83 0.05 251 124 128 4 811.85 6.04 252 125 128 3 824.41 4.88 253 125 129 4 807.13 1.30 254 126 129 3 821.22 0.55 255 126 130 4 807.12 1.31 256 127 130 3 818.77 1.41 257 127 131 4 818.77 1.40 258 128 131 3 827.32 2.03 259 128 132 4 818.78 1.40 260 128 138 10 528.48 4.54 261 129 132 3 827.31 2.04 262 129 138 9 528.29 4.59 263 132 136 4 756.47 13.34 264 132 137 5 710.19 8.89 265 132 138 6 689.77 1.31 266 133 136 3 817.42 0.88 267 146 279 133 35.18 0.21 268 147 153 6 803.84 1.35 269 147 157 10 622.12 51.15 270 147 172 25 442.41 0.21 271 148 153 5 803.84 1.35 272 149 153 4 822.60 3.52 273 149 176 27 441.09 0.10 274 149 179 30 436.09 0.82 275 149 182 33 419.63 1.21 276 150 153 3 826.57 1.54 277 151 179 28 438.25 0.27 278 151 181 30 428.32 0.66 279 151 182 31 419.63 1.21 280 152 181 29 433.49 1.84 281 152 182 30 415.05 1.09 282 152 183 31 411.51 0.67 283 152 184 32 399.98 3.15 284 153 157 4 888.02 3.31 285 154 157 3 889.95 2.35 286 154 184 30 377.89 1.27 287 154 249 95 125.14 0.56 288 154 250 96 125.11 0.55 289 154 251 97 125.09 0.56 290 155 184 29 377.57 1.39 291 155 185 30 377.57 1.39 292 155 249 94 125.24 0.51 293 156 184 28 376.79 1.01 294 156 185 29 370.97 3.91 295 156 187 31 370.72 3.90 296 156 243 87 151.43 10.47 297 156 244 88 151.40 10.46 298 156 249 93 125.25 0.51 299 156 250 94 125.11 0.55 300 156 251 95 125.11 0.55 301 157 172 15 442.81 0.07 302 157 176 19 442.50 0.15 303 157 184 27 376.79 1.01 304 157 185 28 370.87 3.96 305 157 186 29 369.48 3.54 306 157 187 30 369.47 3.53 307 157 251 94 125.01 0.56 308 157 253 96 124.84 0.59 309 158 187 29 366.01 2.01 310 158 243 85 151.41 10.46 311 158 250 92 125.12 0.55 312 158 251 93 125.09 0.56 313 158 252 94 124.82 0.59 314 158 253 95 124.84 0.59 315 159 169 10 442.93 0.03 316 159 172 13 442.93 0.03 317 159 173 14 442.90 0.03 318 159 186 27 366.63 2.30 319 159 187 28 365.96 1.99 320 159 188 29 352.33 8.63 321 159 189 30 351.10 9.25 322 159 243 84 151.44 10.48 323 159 253 94 124.72 0.62 324 160 169 9 443.25 0.14 325 160 189 29 332.36 0.12 326 160 234 74 161.03 6.25 327 160 239 79 160.66 6.21 328 160 243 83 151.56 10.42 329 160 252 92 124.83 0.60 330 160 253 93 124.81 0.60 331 160 254 94 123.60 0.30 332 160 255 95 122.36 0.90 333 160 260 100 115.12 0.16 334 160 264 104 102.00 0.94 335 161 165 4 769.93 16.36 336 161 166 5 711.12 13.04 337 161 169 8 443.25 0.14 338 161 188 27 340.22 2.54 339 161 189 28 332.34 0.10 340 161 190 29 332.01 0.09 341 161 191 30 331.83 0.18 342 161 255 94 119.84 0.34 343 161 260 99 111.81 1.25 344 162 165 3 824.12 0.70 345 162 191 29 331.81 0.19 346 162 255 93 121.71 0.81 347 162 256 94 119.22 0.29 348 162 257 95 119.22 0.29 349 162 260 98 109.84 0.26 350 163 166 3 855.55 4.84 351 163 190 27 332.08 0.12 352 163 191 28 331.85 0.20 353 163 192 29 330.45 0.85 354 163 199 36 324.31 1.84 355 164 167 3 853.47 1.42 356 164 168 4 849.50 0.57 357 164 199 35 324.31 1.84 358 165 168 3 869.07 1.01 359 165 169 4 835.50 0.81 360 165 255 90 119.20 0.29 361 165 256 91 119.19 0.29 362 166 169 3 835.50 0.81 363 166 170 4 831.28 0.95 364 166 188 22 340.19 2.55 365 166 190 24 331.82 0.18 366 166 199 33 324.27 1.86 367 166 200 34 324.26 1.86 368 166 204 38 322.99 1.24 369 167 170 3 835.50 0.81 370 167 171 4 828.41 2.39 371 167 200 33 324.25 1.86 372 168 171 3 830.15 2.25 373 168 172 4 824.57 0.47 374 168 255 87 119.20 0.29 375 168 281 113 15.19 0.79 376 169 172 3 827.43 0.96 377 169 173 4 809.73 0.99 378 169 188 19 338.91 3.15 379 169 253 84 122.38 0.91 380 169 254 85 122.29 0.87 381 169 255 86 119.22 0.29 382 169 281 112 33.20 1.15 383 170 173 3 821.35 1.83 384 170 174 4 809.74 0.99 385 170 200 30 324.24 1.87 386 170 204 34 321.30 1.79 387 170 207 37 314.33 0.21 388 171 174 3 834.55 7.95 389 171 175 4 817.61 2.94 390 172 175 3 827.62 7.94 391 172 176 4 817.60 2.93 392 172 253 81 122.34 0.89 393 172 281 109 32.95 1.27 394 173 176 3 822.90 0.29 395 173 177 4 817.60 2.93 396 173 186 13 365.91 1.95 397 173 207 34 313.51 0.20 398 173 214 41 305.73 2.46 399 174 177 3 841.31 2.52 400 174 178 4 817.50 2.98 401 174 203 29 322.73 1.24 402 174 207 33 313.50 0.20 403 175 178 3 825.60 1.62 404 175 179 4 817.50 2.97 405 176 179 3 820.55 0.88 406 176 182 6 545.35 69.89 407 176 184 8 376.74 0.98 408 176 186 10 370.20 4.09 409 176 213 37 306.39 2.36 410 177 207 30 313.64 0.12 411 177 211 34 306.48 2.33 412 177 213 36 306.38 2.37 413 177 214 37 306.35 2.37 414 178 211 33 306.51 2.33 415 179 182 3 822.64 12.20 416 184 213 29 306.40 2.35 417 185 213 28 306.38 2.35 418 186 213 27 306.38 2.36 419 186 214 28 305.92 2.46 420 186 215 29 305.82 2.51 421 187 215 28 305.69 2.45 422 187 242 55 151.57 10.43 423 187 243 56 151.44 10.48 424 188 204 16 323.35 1.34 425 188 214 26 305.94 2.48 426 188 215 27 305.65 2.46 427 188 216 28 302.06 0.98 428 188 217 29 300.99 1.52 429 189 217 28 298.03 0.28 430 189 219 30 294.81 1.00 431 189 234 45 161.09 6.22 432 189 238 49 160.84 6.23 433 189 239 50 160.67 6.21 434 189 242 53 157.19 7.67 435 189 243 54 151.56 10.42 436 190 198 8 324.70 1.71 437 190 199 9 324.69 1.71 438 190 204 14 324.17 1.75 439 190 216 26 302.06 0.98 440 190 217 27 297.94 0.32 441 190 218 28 293.07 1.74 442 190 219 29 292.27 2.10 443 191 219 28 283.89 2.10 444 191 234 43 161.03 6.25 445 191 260 69 116.94 0.69 446 192 197 5 324.76 1.71 447 192 198 6 324.71 1.71 448 192 219 27 285.61 1.24 449 192 220 28 281.55 1.04 450 192 221 29 281.53 1.05 451 193 197 4 784.74 8.36 452 193 220 27 281.76 0.97 453 193 221 28 281.29 0.93 454 193 222 29 281.24 0.95 455 194 197 3 812.12 2.73 456 194 220 26 281.62 1.03 457 194 222 28 281.22 0.96 458 195 198 3 848.33 3.66 459 195 201 6 784.24 8.12 460 195 220 25 281.62 1.03 461 195 224 29 218.86 4.09 462 198 201 3 836.99 0.94 463 198 218 20 293.03 1.74 464 198 220 22 281.62 1.03 465 198 227 29 200.11 7.96 466 199 204 5 807.51 2.09 467 200 203 3 814.41 0.59 468 200 204 4 807.51 2.09 469 201 204 3 832.43 0.82 470 201 205 4 807.52 2.10 471 201 218 17 297.87 0.29 472 201 227 26 200.10 7.96 473 202 205 3 820.07 3.43 474 202 206 4 807.52 2.10 475 203 206 3 826.49 3.79 476 203 207 4 807.11 2.31 477 204 207 3 831.65 2.58 478 204 208 4 807.87 1.93 479 204 214 10 305.78 2.49 480 204 216 12 302.06 0.98 481 204 218 14 298.08 0.31 482 205 208 3 824.08 5.70 483 205 209 4 817.48 2.95 484 205 216 11 305.26 2.47 485 205 218 13 302.16 0.93 486 206 209 3 825.15 0.89 487 206 210 4 807.03 2.27 488 207 210 3 821.08 2.94 489 207 211 4 801.00 5.27 490 207 214 7 305.82 2.44 491 208 211 3 821.56 5.01 492 208 214 6 305.89 2.41 493 208 216 8 305.72 2.48 494 211 214 3 823.65 6.67 495 217 229 12 194.81 10.41 496 217 230 13 194.81 10.40 497 217 238 21 160.97 6.27 498 217 242 25 158.18 7.18 499 218 227 9 200.07 7.98 500 218 229 11 194.81 10.41 501 218 230 12 194.81 10.40 502 219 230 11 194.74 10.38 503 219 231 12 194.72 10.37 504 219 232 13 194.52 10.28 505 219 233 14 194.25 10.19 506 219 234 15 186.32 6.33 507 219 238 19 164.18 4.67 508 220 223 3 811.79 3.52 509 220 224 4 844.20 0.46 510 220 227 7 200.15 7.95 511 220 230 10 194.81 10.40 512 220 231 11 194.74 10.38 513 220 232 12 194.50 10.29 514 221 232 11 194.46 10.29 515 221 233 12 194.46 10.29 516 223 227 4 723.50 9.27 517 223 231 8 194.80 10.40 518 224 227 3 822.28 2.06 519 227 230 3 1028.02 0.10 520 227 231 4 851.40 3.16 521 230 238 8 172.85 0.38 522 231 238 7 772.81 11.04 523 233 238 5 814.14 6.67 524 234 238 4 813.62 6.92 525 234 239 5 798.29 0.74 526 234 260 26 119.28 0.26 527 234 264 30 105.80 0.49 528 234 267 33 102.00 0.99 529 235 238 3 824.99 4.17 530 235 239 4 820.31 3.58 531 235 267 32 102.11 1.05 532 236 239 3 833.82 3.16 533 236 240 4 813.63 6.92 534 236 267 31 101.78 1.04 535 236 270 34 98.94 2.19 536 236 271 35 93.67 0.95 537 237 240 3 820.58 3.45 538 237 241 4 810.42 0.39 539 238 241 3 822.08 2.71 540 238 242 4 814.24 1.50 541 239 242 3 818.42 0.88 542 239 243 4 778.22 8.78 543 239 264 25 105.72 0.52 544 239 267 28 101.73 1.05 545 239 268 29 101.56 1.12 546 239 271 32 92.45 1.40 547 240 243 3 836.63 3.04 548 240 271 31 90.86 0.57 549 243 250 7 161.59 7.49 550 243 252 9 125.20 0.54 551 243 273 30 80.75 0.79 552 244 248 4 724.20 6.90 553 244 250 6 161.09 7.24 554 244 273 29 80.75 0.81 555 245 248 3 804.12 1.57 556 245 250 5 178.75 11.30 557 245 273 28 80.74 0.81 558 246 271 25 86.61 0.43 559 246 272 26 82.17 1.30 560 246 273 27 79.01 1.67 561 246 277 31 62.83 0.70 562 250 273 23 76.34 2.84 563 250 277 27 45.20 0.48 564 251 276 25 52.60 0.99 565 251 277 26 45.20 0.47 566 251 278 27 45.07 0.50 567 252 268 16 93.70 0.95 568 252 273 21 67.95 0.73 569 252 277 25 45.26 0.45 570 252 278 26 44.92 0.49 571 252 279 27 44.91 0.50 572 253 279 26 44.68 0.42 573 253 281 28 38.50 0.24 574 254 264 10 102.22 1.04 575 254 268 14 98.28 2.56 576 254 278 24 44.98 0.52 577 254 279 25 44.84 0.47 578 254 280 26 37.80 0.09 579 254 281 27 35.71 0.47 580 255 261 6 717.93 8.53 581 255 281 26 31.97 0.55 582 256 261 5 789.16 1.93 583 257 260 3 805.95 4.12 584 257 261 4 802.77 3.04 585 258 261 3 810.78 0.96 586 258 262 4 802.77 3.05 587 259 262 3 805.01 4.17 588 259 263 4 802.76 3.05 589 260 263 3 817.00 1.08 590 260 264 4 813.91 0.55 591 261 264 3 815.92 0.54 592 261 265 4 816.12 0.66 593 261 280 19 44.84 0.47 594 262 265 3 830.39 4.11 595 262 266 4 816.15 0.64 596 263 266 3 816.13 0.65 597 263 267 4 815.12 1.16 598 264 267 3 818.50 0.54 599 264 268 4 815.10 1.16 600 265 268 3 816.12 0.63 601 265 269 4 804.47 6.28 602 265 278 13 45.30 0.43 603 266 269 3 823.76 3.20 604 266 270 4 800.15 4.12 605 267 270 3 812.15 1.36 606 267 271 4 800.11 4.16 607 268 271 3 822.88 4.00 608 268 272 4 791.04 8.69 609 268 273 5 719.75 9.55 610 268 278 10 96.03 19.41 611 269 272 3 822.02 4.42 612 269 278 9 170.09 5.80 613 273 277 4 798.65 5.99 614 273 278 5 778.40 5.78 615 274 277 3 826.75 0.27 616 274 278 4 826.88 0.20 617 275 278 3 832.26 2.50