BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 143 142 36.73 1.36 2 2 143 141 37.46 1.34 3 2 217 215 37.45 1.34 4 3 14 11 220.49 92.85 5 3 57 54 130.28 47.74 6 3 139 136 237.79 12.64 7 3 143 140 37.47 1.34 8 3 144 141 37.47 1.34 9 3 147 144 37.47 1.34 10 3 216 213 37.48 1.34 11 3 217 214 37.48 1.34 12 4 139 135 237.97 12.62 13 5 9 4 564.11 55.12 14 5 11 6 593.61 40.38 15 5 137 132 277.00 3.95 16 5 139 134 259.84 1.71 17 6 137 131 332.98 0.45 18 6 138 132 327.55 2.86 19 7 136 129 347.98 2.23 20 7 137 130 343.94 0.26 21 8 11 3 906.20 0.59 22 8 62 54 395.97 0.74 23 8 64 56 397.61 1.36 24 8 65 57 396.61 1.46 25 8 85 77 392.57 0.37 26 8 86 78 388.94 0.48 27 8 136 128 363.15 1.58 28 8 137 129 348.06 2.20 29 10 62 52 398.02 1.26 30 10 63 53 398.09 1.28 31 10 64 54 398.07 1.27 32 11 59 48 398.10 1.22 33 11 60 49 398.06 1.24 34 11 61 50 398.08 1.28 35 11 62 51 398.03 1.26 36 11 137 126 396.40 1.66 37 11 139 128 396.06 1.82 38 11 213 202 137.53 0.61 39 12 212 200 137.64 0.62 40 12 213 201 137.55 0.61 41 12 226 214 108.06 0.12 42 13 59 46 398.22 1.24 43 13 60 47 398.19 1.25 44 13 211 198 139.07 0.01 45 13 212 199 137.65 0.62 46 13 213 200 137.59 0.61 47 13 226 213 109.03 0.34 48 13 228 215 106.97 0.39 49 14 57 43 398.56 1.29 50 14 58 44 398.31 1.19 51 14 59 45 398.23 1.24 52 14 139 125 397.18 1.69 53 14 210 196 146.20 0.83 54 14 211 197 140.00 0.42 55 14 213 199 137.75 0.58 56 14 216 202 137.59 0.59 57 15 57 42 398.79 1.25 58 15 58 43 398.57 1.31 59 15 208 193 155.75 0.73 60 15 209 194 155.69 0.71 61 15 210 195 149.85 0.85 62 16 56 40 399.01 1.24 63 16 57 41 398.88 1.28 64 16 148 132 385.38 1.82 65 16 151 135 383.57 1.54 66 16 152 136 383.46 1.57 67 16 155 139 360.45 0.92 68 16 208 192 157.41 1.56 69 16 209 193 155.74 0.74 70 16 211 195 155.05 0.44 71 17 55 38 399.26 1.22 72 17 56 39 399.06 1.26 73 17 58 41 398.99 1.30 74 17 206 189 161.29 2.79 75 17 207 190 161.12 2.75 76 17 208 191 157.41 1.56 77 18 52 34 401.51 1.17 78 18 53 35 401.33 1.08 79 18 54 36 399.49 1.17 80 18 55 37 399.44 1.19 81 18 152 134 383.58 1.54 82 18 155 137 376.12 1.89 83 18 206 188 161.38 2.83 84 18 207 189 161.16 2.79 85 18 209 191 159.28 1.96 86 19 24 5 778.50 7.49 87 19 51 32 402.21 1.15 88 19 52 33 401.94 1.09 89 19 53 34 401.40 1.11 90 19 55 36 400.14 0.85 91 19 205 186 164.17 3.98 92 19 206 187 163.68 3.98 93 20 23 3 834.20 1.36 94 20 24 4 816.10 0.57 95 20 48 28 402.24 1.15 96 20 51 31 402.21 1.15 97 20 52 32 401.95 1.08 98 20 164 144 312.62 2.33 99 20 168 148 309.98 1.85 100 20 204 184 164.20 3.97 101 20 205 185 164.21 3.97 102 20 206 186 164.18 3.98 103 20 240 220 79.22 0.41 104 20 241 221 77.47 1.04 105 21 24 3 808.05 3.46 106 21 25 4 807.54 3.71 107 21 51 30 402.22 1.14 108 21 53 32 402.17 1.16 109 21 117 96 398.56 2.96 110 22 26 4 807.53 3.71 111 22 51 29 402.28 1.11 112 22 168 146 310.02 1.83 113 22 241 219 79.00 0.29 114 22 242 220 75.94 1.24 115 22 243 221 60.63 0.06 116 23 26 3 815.96 0.53 117 23 27 4 785.36 11.03 118 23 203 180 169.58 5.63 119 23 205 182 165.66 4.72 120 23 241 218 77.81 0.89 121 24 27 3 834.08 3.96 122 25 29 4 817.89 2.17 123 25 39 14 402.47 1.18 124 25 245 220 53.85 1.60 125 26 29 3 821.25 0.81 126 26 30 4 804.37 4.59 127 26 203 177 170.13 5.46 128 26 241 215 83.39 0.01 129 26 245 219 38.20 7.37 130 27 30 3 820.65 0.85 131 27 31 4 804.37 4.59 132 27 198 171 169.81 5.70 133 27 203 176 170.13 5.46 134 28 31 3 817.88 2.16 135 28 32 4 804.31 4.62 136 28 33 5 749.66 1.98 137 28 34 6 723.33 3.98 138 28 36 8 589.03 52.99 139 29 32 3 808.30 2.25 140 29 245 216 59.55 1.19 141 30 203 173 170.58 5.32 142 31 198 167 170.58 5.32 143 35 39 4 825.86 1.29 144 35 245 210 75.97 1.77 145 36 39 3 862.26 4.47 146 37 40 3 845.14 4.09 147 38 41 3 850.16 1.58 148 38 117 79 397.48 2.98 149 38 118 80 393.95 4.74 150 39 42 3 840.52 6.41 151 39 245 206 76.52 1.60 152 42 245 203 76.62 1.56 153 44 51 7 451.34 7.20 154 44 243 199 79.11 0.32 155 47 50 3 827.05 1.41 156 48 51 3 854.83 3.52 157 48 165 117 312.62 2.33 158 48 168 120 311.56 2.44 159 49 52 3 855.39 3.81 160 49 156 107 383.54 1.55 161 49 161 112 331.07 0.66 162 49 165 116 312.60 2.31 163 50 53 3 871.68 2.58 164 50 156 106 383.53 1.55 165 51 168 117 311.56 2.44 166 51 243 192 70.53 1.79 167 52 152 100 385.29 1.81 168 52 155 103 383.55 1.55 169 52 156 104 382.80 1.37 170 52 164 112 312.63 2.32 171 52 207 155 164.17 3.98 172 53 115 62 406.84 7.45 173 53 117 64 407.30 7.32 174 53 152 99 385.06 1.92 175 54 115 61 406.87 7.46 176 54 116 62 406.94 7.42 177 54 148 94 394.43 2.21 178 54 149 95 390.70 4.08 179 54 152 98 383.57 1.53 180 55 116 61 406.97 7.50 181 55 129 74 407.75 7.08 182 55 206 151 163.16 3.73 183 56 129 73 407.61 7.16 184 56 148 92 390.31 4.26 185 57 133 76 409.48 7.85 186 57 144 87 396.06 1.83 187 57 147 90 387.88 0.64 188 57 148 91 385.31 1.79 189 57 151 94 383.58 1.54 190 57 216 159 137.68 0.55 191 58 129 71 408.93 8.12 192 58 133 75 409.44 7.90 193 58 208 150 155.76 0.73 194 59 75 16 439.42 2.04 195 59 133 74 409.18 8.04 196 59 137 78 409.57 7.85 197 59 139 80 409.56 7.85 198 60 75 15 434.07 0.64 199 61 65 4 820.96 1.27 200 61 66 5 810.69 1.65 201 61 70 9 499.72 5.29 202 61 71 10 499.63 5.25 203 61 72 11 474.39 7.28 204 61 75 14 434.07 0.64 205 62 65 3 827.42 2.02 206 62 66 4 820.99 1.28 207 62 75 13 474.75 7.10 208 62 137 75 426.43 0.03 209 63 66 3 828.84 5.11 210 63 67 4 815.93 3.81 211 64 67 3 823.72 0.08 212 64 68 4 822.47 0.55 213 65 68 3 825.94 1.19 214 65 70 5 760.15 6.27 215 65 75 10 515.31 2.28 216 65 82 17 515.29 2.30 217 65 85 20 508.44 1.12 218 65 86 21 508.43 1.11 219 66 69 3 832.69 2.19 220 66 70 4 739.19 4.17 221 68 84 16 577.59 4.32 222 68 85 17 576.79 3.92 223 69 74 5 826.85 1.40 224 70 74 4 819.28 2.39 225 70 75 5 806.44 4.01 226 70 82 12 599.31 0.53 227 70 83 13 600.31 1.02 228 70 84 14 600.29 1.02 229 71 74 3 826.86 1.40 230 71 75 4 818.27 1.88 231 72 75 3 826.84 1.39 232 72 76 4 818.27 1.88 233 73 76 3 826.88 1.69 234 73 77 4 817.86 1.70 235 74 77 3 825.75 2.27 236 74 78 4 810.71 5.25 237 74 82 8 625.07 2.15 238 74 83 9 625.23 2.23 239 74 84 10 625.50 2.37 240 75 78 3 825.75 2.27 241 75 79 4 800.14 7.19 242 75 80 5 807.34 3.58 243 75 82 7 625.08 2.15 244 75 86 11 625.68 2.49 245 75 137 62 623.70 2.25 246 76 132 56 623.90 2.78 247 77 132 55 624.56 2.59 248 78 81 3 823.22 1.95 249 78 82 4 723.18 1.46 250 78 90 12 624.70 2.63 251 78 132 54 624.56 2.59 252 79 82 3 830.06 4.51 253 79 86 7 673.40 26.36 254 79 132 53 624.56 2.58 255 80 86 6 625.71 2.51 256 80 87 7 625.70 2.51 257 80 88 8 625.07 2.63 258 80 131 51 624.64 2.60 259 80 132 52 624.63 2.60 260 80 133 53 624.60 2.58 261 80 134 54 624.69 2.61 262 80 135 55 624.74 2.61 263 80 136 56 624.74 2.61 264 80 137 57 624.75 2.61 265 81 87 6 625.67 2.52 266 81 88 7 625.40 2.41 267 81 89 8 624.98 2.58 268 81 90 9 624.75 2.60 269 82 85 3 862.68 3.52 270 82 86 4 813.24 0.47 271 82 87 5 711.07 7.51 272 83 87 4 805.73 4.23 273 84 87 3 863.61 0.46 274 86 136 50 624.68 2.65 275 88 135 47 624.78 2.60 276 88 136 48 624.78 2.60 277 91 99 8 624.93 2.60 278 91 128 37 624.83 2.64 279 91 131 40 624.85 2.63 280 92 95 3 828.88 1.24 281 92 96 4 769.03 5.95 282 92 128 36 625.00 2.64 283 93 96 3 831.05 0.41 284 93 128 35 626.88 2.77 285 95 98 3 840.52 0.73 286 96 99 3 840.52 0.73 287 96 123 27 640.82 2.76 288 96 124 28 629.18 3.06 289 96 125 29 629.16 3.06 290 96 127 31 629.17 3.06 291 96 128 32 629.16 3.06 292 97 122 25 649.15 0.96 293 97 123 26 646.81 0.22 294 97 124 27 629.46 2.92 295 97 127 30 640.81 2.75 296 99 127 28 671.50 1.72 297 99 128 29 672.73 2.37 298 99 131 32 672.31 1.96 299 100 127 27 673.12 2.18 300 101 127 26 673.38 2.06 301 102 126 24 673.62 1.97 302 102 127 25 673.62 1.97 303 102 130 28 673.62 1.97 304 102 141 39 673.49 2.00 305 102 145 43 673.51 2.01 306 104 108 4 800.84 0.59 307 104 109 5 794.25 2.69 308 104 112 8 777.39 2.49 309 104 126 22 674.12 1.93 310 104 141 37 673.82 1.99 311 104 142 38 673.73 1.95 312 104 145 41 673.81 1.99 313 105 108 3 821.25 3.28 314 105 109 4 813.70 7.03 315 105 142 37 674.03 1.89 316 106 109 3 821.23 3.26 317 106 110 4 813.73 7.03 318 107 110 3 828.56 0.39 319 107 111 4 815.50 6.15 320 108 111 3 820.70 3.55 321 108 112 4 815.49 6.14 322 108 142 34 674.63 1.78 323 108 145 37 674.60 1.79 324 108 146 38 674.60 1.79 325 109 112 3 820.09 3.85 326 109 113 4 819.47 4.16 327 110 113 3 828.38 1.46 328 110 114 4 820.71 3.50 329 111 114 3 823.41 2.15 330 111 115 4 820.71 3.50 331 111 149 38 674.65 1.80 332 112 115 3 832.36 2.15 333 112 116 4 798.22 0.93 334 112 119 7 675.50 1.66 335 112 126 14 675.46 1.64 336 112 145 33 674.68 1.81 337 112 149 37 674.65 1.80 338 113 116 3 820.63 3.46 339 113 119 6 675.47 1.65 340 115 149 34 674.66 1.80 341 115 152 37 674.65 1.80 342 115 156 41 672.30 2.73 343 116 119 3 822.82 3.52 344 116 125 9 676.03 1.73 345 116 126 10 676.02 1.73 346 116 145 29 674.86 1.82 347 116 148 32 674.76 1.79 348 118 123 5 676.12 1.77 349 118 124 6 676.13 1.77 350 118 125 7 676.03 1.73 351 119 125 6 676.13 1.77 352 119 126 7 676.13 1.77 353 120 123 3 836.88 0.33 354 120 124 4 676.21 1.79 355 120 126 6 676.20 1.80 356 121 124 3 848.56 0.32 357 124 127 3 821.91 2.08 358 124 128 4 813.22 3.66 359 125 128 3 838.57 0.18 360 125 129 4 803.89 1.31 361 126 129 3 813.66 6.20 362 126 130 4 803.89 1.31 363 126 145 19 674.91 1.82 364 127 130 3 825.86 0.12 365 127 131 4 800.37 0.45 366 128 131 3 834.77 0.43 367 128 132 4 800.32 0.43 368 129 132 3 820.91 2.35 369 129 133 4 796.07 0.72 370 130 133 3 820.02 6.86 371 130 134 4 775.50 0.19 372 130 135 5 769.19 2.97 373 130 139 9 677.86 1.05 374 130 141 11 675.25 1.84 375 130 144 14 675.20 1.86 376 130 145 15 675.06 1.85 377 131 135 4 781.52 4.69 378 133 137 4 832.16 4.37 379 133 139 6 795.06 2.07 380 133 144 11 675.38 1.90 381 134 137 3 832.16 4.37 382 134 138 4 807.71 0.73 383 134 139 5 760.58 1.32 384 135 138 3 814.61 5.55 385 139 144 5 773.53 9.46 386 140 143 3 818.35 2.49 387 140 144 4 796.67 2.09 388 141 144 3 808.53 2.42 389 141 145 4 798.12 1.35 390 142 146 4 798.15 1.32 391 143 146 3 818.23 7.17 392 143 147 4 798.48 1.16 393 144 147 3 838.41 2.91 394 144 148 4 816.52 8.03 395 145 148 3 825.51 3.54 396 145 149 4 806.31 2.90 397 146 149 3 828.93 0.42 398 146 150 4 810.75 0.69 399 147 150 3 825.83 0.35 400 147 151 4 806.90 1.24 401 147 216 69 138.87 0.04 402 148 151 3 815.67 4.81 403 148 152 4 806.88 1.24 404 149 152 3 835.03 2.24 405 149 153 4 815.94 5.86 406 150 153 3 827.25 0.20 407 150 154 4 815.94 5.86 408 150 216 66 139.30 0.04 409 150 217 67 139.19 0.04 410 150 218 68 139.12 0.03 411 150 219 69 138.74 0.00 412 151 154 3 819.16 4.25 413 151 155 4 815.93 5.86 414 151 211 60 155.85 0.69 415 151 216 65 139.22 0.05 416 151 220 69 137.42 0.53 417 152 155 3 816.18 2.76 418 152 156 4 816.18 2.76 419 153 156 3 817.58 2.06 420 153 157 4 808.32 1.18 421 154 157 3 819.10 0.48 422 154 158 4 805.78 2.44 423 154 219 65 139.08 0.04 424 154 220 66 138.05 0.35 425 155 158 3 812.32 2.91 426 155 161 6 741.43 1.48 427 155 209 54 161.31 2.78 428 155 220 65 138.03 0.36 429 156 161 5 735.11 4.63 430 156 162 6 761.39 4.17 431 157 162 5 794.13 1.91 432 158 161 3 811.70 2.89 433 158 162 4 817.32 6.36 434 158 184 26 185.53 15.52 435 158 185 27 185.56 15.51 436 159 162 3 819.98 5.29 437 159 163 4 805.42 0.24 438 159 180 21 185.77 15.62 439 159 183 24 185.72 15.60 440 159 184 25 185.53 15.52 441 160 163 3 823.77 3.14 442 160 164 4 817.98 6.04 443 160 180 20 185.77 15.62 444 160 181 21 185.50 15.49 445 161 164 3 822.54 3.77 446 161 165 4 817.50 6.29 447 162 165 3 821.13 4.56 448 162 166 4 805.37 0.23 449 163 166 3 816.89 1.34 450 163 167 4 803.84 0.54 451 163 172 9 458.85 11.70 452 163 176 13 198.34 9.54 453 163 177 14 186.59 15.39 454 163 180 17 186.35 15.40 455 164 167 3 811.74 2.75 456 164 168 4 803.67 0.61 457 164 177 13 187.28 15.04 458 164 204 40 169.22 5.92 459 164 240 76 83.41 0.01 460 165 168 3 814.24 1.98 461 166 170 4 854.94 1.22 462 167 170 3 820.93 2.14 463 167 172 5 775.66 5.43 464 167 240 73 84.30 0.01 465 168 240 72 84.31 0.01 466 168 241 73 84.17 0.04 467 168 242 74 83.51 0.03 468 168 243 75 83.52 0.03 469 169 240 71 84.34 0.01 470 169 242 73 83.52 0.02 471 170 239 69 94.77 1.04 472 170 240 70 88.12 0.77 473 171 237 66 101.06 0.83 474 171 238 67 99.82 1.09 475 171 239 68 95.84 0.50 476 172 237 65 100.33 1.16 477 172 238 66 99.39 0.95 478 172 240 68 95.75 0.47 479 173 176 3 819.12 3.08 480 173 177 4 815.13 5.09 481 173 236 63 101.08 0.79 482 173 237 64 98.61 0.56 483 174 177 3 817.10 4.12 484 174 178 4 817.10 4.11 485 174 195 21 191.16 12.16 486 174 231 57 114.89 0.95 487 174 232 58 114.89 0.94 488 174 233 59 114.84 0.93 489 174 234 60 102.28 0.96 490 174 235 61 101.09 0.79 491 174 236 62 100.17 1.17 492 174 238 64 99.18 0.85 493 175 178 3 825.28 0.07 494 175 179 4 817.12 4.12 495 176 179 3 820.24 2.59 496 176 180 4 817.13 4.12 497 177 180 3 831.96 3.27 498 177 181 4 820.21 2.58 499 177 193 16 213.81 1.20 500 177 195 18 190.00 11.12 501 177 238 61 98.24 0.74 502 178 181 3 820.38 2.67 503 178 182 4 815.40 4.99 504 178 190 12 213.83 1.23 505 179 182 3 815.32 4.94 506 179 183 4 816.86 4.16 507 180 183 3 816.99 4.08 508 180 184 4 816.99 4.08 509 181 184 3 835.14 2.68 510 181 185 4 815.26 4.92 511 181 189 8 581.13 49.98 512 181 190 9 234.75 3.99 513 181 193 12 220.94 2.90 514 181 225 44 122.38 0.44 515 182 185 3 827.44 1.17 516 182 186 4 747.39 8.56 517 182 187 5 467.09 6.25 518 182 190 8 244.59 5.37 519 185 189 4 813.07 3.29 520 185 220 35 138.88 0.01 521 185 221 36 137.57 0.53 522 185 222 37 131.28 1.48 523 185 225 40 123.86 0.29 524 186 189 3 837.94 1.39 525 186 190 4 814.20 2.73 526 187 190 3 814.20 2.73 527 189 192 3 828.78 2.58 528 189 222 33 125.07 1.79 529 189 225 36 115.35 1.07 530 190 193 3 848.62 2.97 531 191 227 36 114.92 0.96 532 191 228 37 114.89 0.95 533 192 208 16 186.32 6.97 534 192 209 17 177.16 4.45 535 192 210 18 174.60 5.71 536 192 225 33 115.08 1.00 537 192 226 34 114.92 0.96 538 192 227 35 114.87 0.95 539 192 228 36 114.87 0.96 540 193 207 14 186.75 6.76 541 193 208 15 186.19 7.03 542 193 209 16 177.17 4.45 543 193 228 35 114.86 0.95 544 193 231 38 102.12 1.20 545 194 207 13 187.92 6.21 546 194 208 14 186.21 7.04 547 194 228 34 114.83 0.95 548 194 230 36 102.98 1.01 549 194 231 37 102.12 1.20 550 194 232 38 101.35 0.88 551 195 204 9 232.75 1.21 552 195 206 11 225.50 2.04 553 195 207 12 187.69 6.35 554 195 231 36 102.23 1.19 555 195 232 37 101.52 0.95 556 195 233 38 101.35 0.88 557 195 234 39 101.11 0.81 558 195 238 43 84.42 0.04 559 196 204 8 232.75 1.21 560 196 205 9 232.69 1.21 561 196 206 10 225.61 2.00 562 196 233 37 101.56 0.97 563 196 234 38 101.30 0.90 564 197 202 5 584.56 58.50 565 197 203 6 425.95 2.17 566 197 204 7 232.75 1.21 567 197 205 8 232.73 1.22 568 197 233 36 102.09 1.20 569 197 234 37 101.30 0.90 570 197 238 41 84.73 0.17 571 198 202 4 765.53 1.61 572 198 203 5 490.72 16.46 573 198 205 7 337.20 50.10 574 199 202 3 806.90 0.29 575 199 203 4 775.71 3.47 576 202 234 32 102.14 1.19 577 202 238 36 84.78 0.15 578 202 239 37 84.74 0.17 579 203 239 36 84.28 0.01 580 203 241 38 77.88 0.84 581 204 207 3 996.21 1.78 582 204 234 30 101.53 1.02 583 204 238 34 84.35 0.01 584 204 239 35 84.17 0.05 585 204 240 36 79.78 0.04 586 209 225 16 114.64 1.02 587 210 225 15 111.19 0.51 588 210 226 16 109.98 0.06 589 210 228 18 107.81 0.04 590 211 215 4 812.41 1.66 591 211 216 5 776.36 4.32 592 211 220 9 163.62 21.15 593 211 224 13 111.21 0.49 594 211 225 14 110.00 0.05 595 212 215 3 812.37 1.68 596 212 216 4 812.32 1.71 597 212 223 11 114.62 1.02 598 212 224 12 111.09 0.55 599 212 225 13 110.18 0.14 600 212 226 14 109.15 0.40 601 213 216 3 828.87 6.57 602 215 218 3 820.55 8.21 603 215 224 9 110.18 0.14 604 220 224 4 771.63 4.60 605 220 225 5 771.63 4.60 606 221 224 3 821.57 2.39 607 221 225 4 831.43 2.54 608 234 237 3 886.03 0.94 609 234 238 4 693.96 33.26