BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 38 41 3 822.43 4.34 2 38 82 44 191.53 0.47 3 38 177 139 191.52 0.47 4 39 62 23 192.22 0.31 5 39 64 25 192.13 0.35 6 39 65 26 191.70 0.38 7 39 66 27 191.53 0.47 8 39 80 41 191.44 0.45 9 39 81 42 191.44 0.45 10 39 82 43 191.52 0.47 11 39 87 48 192.38 0.33 12 39 131 92 192.23 0.30 13 40 43 3 781.23 6.34 14 40 59 19 192.22 0.31 15 40 61 21 192.25 0.30 16 40 66 26 192.22 0.30 17 40 78 38 192.07 0.35 18 40 80 40 191.90 0.43 19 40 259 219 51.95 0.10 20 41 177 136 193.37 0.62 21 41 213 172 195.03 0.23 22 41 214 173 195.03 0.23 23 41 259 218 51.96 0.10 24 42 177 135 193.40 0.62 25 42 179 137 193.37 0.62 26 42 205 163 193.38 0.62 27 42 217 175 193.84 0.83 28 42 257 215 54.63 1.42 29 42 258 216 54.65 1.43 30 42 259 217 51.98 0.12 31 43 258 215 54.09 1.16 32 43 259 216 51.95 0.10 33 44 47 3 802.25 1.98 34 44 48 4 768.82 0.47 35 44 59 15 197.15 0.04 36 44 78 34 195.36 0.03 37 44 258 214 54.12 1.17 38 45 78 33 195.36 0.04 39 45 179 134 196.28 0.48 40 45 254 209 57.50 2.02 41 45 255 210 57.38 2.00 42 45 256 211 55.83 1.51 43 46 57 11 197.77 0.29 44 46 68 22 197.97 0.18 45 46 78 32 197.13 0.06 46 46 254 208 57.50 2.02 47 47 57 10 201.84 0.22 48 48 57 9 201.50 0.07 49 48 59 11 201.77 0.05 50 48 68 20 201.36 0.01 51 48 78 30 201.35 0.01 52 49 54 5 667.81 49.74 53 49 57 8 202.47 0.04 54 49 58 9 202.48 0.04 55 49 59 10 202.23 0.07 56 50 58 8 202.59 0.07 57 50 59 9 202.50 0.05 58 51 54 3 813.63 2.48 59 51 58 7 473.94 7.67 60 52 55 3 798.00 2.55 61 52 58 6 634.95 66.11 62 52 104 52 503.46 1.63 63 52 107 55 504.64 1.96 64 54 57 3 828.60 5.18 65 54 58 4 762.84 2.37 66 55 58 3 810.58 0.91 67 55 70 15 635.62 65.22 68 55 71 16 635.62 65.22 69 55 107 52 513.86 4.33 70 56 69 13 640.19 62.98 71 56 70 14 635.62 65.21 72 56 71 15 635.62 65.22 73 56 72 16 635.70 65.25 74 56 77 21 640.16 62.94 75 57 68 11 640.27 62.96 76 57 69 12 640.31 63.00 77 57 77 20 640.18 62.94 78 58 68 10 642.67 61.83 79 58 69 11 640.72 62.80 80 58 104 46 526.11 1.27 81 58 107 49 526.03 1.26 82 59 67 8 644.63 60.92 83 59 68 9 643.20 61.59 84 59 78 19 644.58 60.88 85 60 66 6 644.70 60.91 86 60 67 7 644.67 60.92 87 60 69 9 644.65 60.91 88 60 100 40 529.50 0.85 89 60 102 42 531.48 0.24 90 60 103 43 530.64 0.17 91 60 145 85 525.62 1.37 92 61 66 5 645.03 60.75 93 62 65 3 802.18 0.29 94 62 67 5 724.00 21.29 95 62 100 38 537.52 2.67 96 62 102 40 543.34 5.59 97 62 143 81 536.88 2.25 98 64 81 17 665.73 50.29 99 64 87 23 641.26 38.06 100 64 131 67 548.63 7.90 101 64 137 73 548.83 7.99 102 64 140 76 548.41 7.99 103 65 79 14 665.87 50.27 104 65 80 15 665.70 50.20 105 65 81 16 665.53 50.20 106 65 126 61 548.34 8.21 107 65 140 75 548.65 8.10 108 65 141 76 548.58 8.11 109 65 143 78 548.60 8.12 110 65 146 81 548.00 8.22 111 66 78 12 667.08 49.74 112 66 79 13 665.84 50.23 113 66 80 14 665.66 50.19 114 67 76 9 667.37 49.77 115 67 78 11 666.95 49.69 116 67 79 12 665.74 50.19 117 67 145 78 546.61 9.11 118 68 76 8 667.39 49.77 119 68 77 9 667.25 49.76 120 68 78 10 667.05 49.68 121 69 74 5 765.08 1.56 122 69 75 6 712.94 27.00 123 69 76 7 667.40 49.77 124 69 77 8 667.39 49.77 125 69 103 34 546.94 9.14 126 69 107 38 546.89 9.16 127 69 110 41 544.35 10.34 128 69 145 76 544.12 10.47 129 70 73 3 811.63 0.83 130 70 74 4 783.80 7.25 131 70 75 5 713.56 27.30 132 70 76 6 667.80 49.57 133 70 77 7 667.48 49.73 134 71 74 3 798.52 0.10 135 71 107 36 544.88 10.17 136 71 193 122 406.15 1.44 137 73 193 120 406.16 1.43 138 73 194 121 405.97 1.43 139 73 195 122 405.50 1.51 140 74 110 36 543.77 10.64 141 74 193 119 406.16 1.44 142 74 194 120 405.91 1.44 143 75 147 72 543.73 10.63 144 75 148 73 543.60 10.59 145 75 149 74 543.60 10.59 146 75 182 107 455.32 1.81 147 75 194 119 405.97 1.41 148 75 195 120 405.75 1.40 149 76 79 3 854.33 0.30 150 76 125 49 544.03 10.52 151 76 145 69 544.22 10.45 152 76 182 106 455.31 1.80 153 77 181 104 455.38 1.79 154 77 182 105 455.32 1.80 155 77 201 124 369.75 0.96 156 77 254 177 69.48 0.28 157 78 179 101 455.45 1.78 158 78 180 102 455.44 1.78 159 78 181 103 455.33 1.81 160 78 201 123 369.62 0.99 161 78 254 176 63.45 0.90 162 79 125 46 544.28 10.26 163 79 126 47 544.33 10.29 164 79 146 67 545.59 9.61 165 79 179 100 455.45 1.78 166 79 180 101 455.42 1.78 167 80 178 98 455.47 1.77 168 80 179 99 455.43 1.78 169 81 125 44 548.40 8.25 170 81 126 45 548.54 8.31 171 81 127 46 548.86 8.23 172 81 130 49 550.37 8.33 173 81 131 50 552.83 9.80 174 81 177 96 455.54 1.79 175 81 178 97 455.50 1.79 176 81 180 99 455.47 1.79 177 81 202 121 367.23 0.43 178 82 87 5 799.81 1.72 179 82 131 49 555.63 10.92 180 82 175 93 455.73 1.84 181 82 176 94 455.67 1.83 182 82 177 95 455.55 1.79 183 83 87 4 810.99 3.52 184 83 88 5 811.00 3.52 185 83 130 47 556.88 11.55 186 83 131 48 558.73 12.00 187 83 166 83 457.91 1.90 188 83 167 84 457.89 1.90 189 83 170 87 457.63 1.90 190 83 175 92 455.72 1.84 191 83 176 93 455.67 1.83 192 83 178 95 455.58 1.80 193 84 87 3 831.01 2.00 194 84 88 4 811.00 3.52 195 84 170 86 457.69 1.88 196 84 175 91 455.69 1.84 197 85 88 3 820.26 1.10 198 85 170 85 457.81 1.93 199 86 133 47 569.16 12.89 200 87 130 43 569.07 12.89 201 87 131 44 568.91 12.97 202 87 132 45 569.14 12.88 203 87 133 46 569.89 12.52 204 87 137 50 569.09 12.90 205 88 129 41 569.14 12.88 206 88 130 42 569.14 12.88 207 88 132 44 569.15 12.88 208 88 133 45 570.15 12.64 209 88 170 82 457.88 1.93 210 89 132 43 570.15 12.63 211 89 133 44 570.18 12.64 212 90 132 42 570.43 12.71 213 91 132 41 570.60 12.69 214 91 134 43 606.74 16.68 215 91 135 44 606.76 16.69 216 91 138 47 583.93 19.09 217 93 128 35 587.69 21.03 218 93 138 45 609.32 17.93 219 93 142 49 608.50 15.83 220 95 142 47 615.63 18.00 221 96 139 43 615.79 18.02 222 96 142 46 615.70 18.01 223 97 112 15 614.91 17.62 224 97 122 25 614.31 18.48 225 97 124 27 615.84 18.01 226 97 142 45 615.77 17.98 227 97 144 47 615.77 17.98 228 97 147 50 615.77 17.98 229 98 112 14 615.87 18.02 230 98 139 41 616.46 17.96 231 98 140 42 616.39 17.93 232 98 142 44 616.15 17.94 233 98 143 45 615.88 18.02 234 98 144 46 615.88 18.02 235 99 109 10 616.19 17.99 236 99 110 11 616.18 17.99 237 99 111 12 616.01 18.01 238 99 112 13 616.04 18.03 239 99 143 44 616.25 17.94 240 99 144 45 615.97 18.00 241 100 103 3 824.23 2.73 242 100 109 9 616.34 17.98 243 100 143 43 616.32 17.92 244 100 145 45 616.26 17.93 245 101 109 8 616.44 17.95 246 103 107 4 795.68 3.23 247 103 109 6 619.87 16.30 248 103 145 42 616.53 17.97 249 104 107 3 829.91 1.36 250 108 114 6 813.02 0.57 251 108 193 85 406.14 1.43 252 109 114 5 819.46 3.17 253 110 114 4 814.30 0.58 254 110 115 5 813.68 0.90 255 110 144 34 618.52 17.64 256 110 193 83 406.15 1.43 257 110 194 84 406.06 1.40 258 111 114 3 825.98 4.07 259 111 144 33 618.75 17.66 260 112 115 3 826.42 0.34 261 112 116 4 813.80 0.33 262 112 122 10 618.62 17.66 263 112 144 32 618.77 17.66 264 113 116 3 813.80 0.33 265 113 191 78 406.69 1.42 266 114 190 76 406.70 1.42 267 114 191 77 406.59 1.45 268 114 193 79 406.51 1.46 269 114 194 80 406.37 1.50 270 115 118 3 806.46 4.00 271 115 121 6 619.03 17.82 272 115 122 7 618.99 17.82 273 115 144 29 619.19 17.73 274 115 147 32 619.07 17.78 275 115 148 33 618.84 17.71 276 115 149 34 618.62 17.66 277 115 190 75 406.72 1.42 278 115 194 79 406.39 1.51 279 116 122 6 621.05 17.83 280 116 191 75 406.78 1.42 281 118 190 72 406.86 1.40 282 119 152 33 618.57 17.65 283 120 149 29 618.94 17.68 284 120 150 30 618.91 17.68 285 120 151 31 618.75 17.61 286 120 190 70 406.88 1.41 287 120 196 76 406.19 1.52 288 120 230 110 282.50 0.67 289 121 148 27 619.33 17.65 290 121 149 28 618.98 17.69 291 121 150 29 618.95 17.68 292 121 155 34 617.51 17.66 293 122 144 22 619.77 17.55 294 122 147 25 619.57 17.62 295 123 147 24 619.71 17.64 296 123 148 25 619.40 17.68 297 123 150 27 619.06 17.62 298 124 127 3 789.39 4.50 299 124 128 4 784.22 5.82 300 124 141 17 620.02 17.60 301 124 142 18 620.02 17.61 302 124 146 22 619.88 17.61 303 124 147 23 619.77 17.64 304 124 159 35 460.08 0.97 305 125 146 21 619.89 17.61 306 125 148 23 619.88 17.61 307 125 180 55 455.38 1.82 308 125 181 56 455.34 1.81 309 125 182 57 455.34 1.80 310 126 141 15 620.08 17.60 311 126 146 20 619.91 17.61 312 127 130 3 805.46 4.78 313 127 141 14 620.28 17.54 314 127 163 36 457.95 1.91 315 127 167 40 457.94 1.90 316 128 131 3 825.68 2.89 317 128 132 4 812.02 0.49 318 128 138 10 620.33 17.53 319 128 141 13 620.32 17.52 320 128 142 14 620.33 17.54 321 129 132 3 835.88 0.82 322 129 167 38 457.94 1.90 323 130 167 37 457.90 1.90 324 131 137 6 621.21 17.20 325 131 138 7 621.18 17.21 326 131 141 10 620.44 17.57 327 132 138 6 624.35 16.33 328 133 137 4 789.09 2.81 329 134 137 3 802.00 3.65 330 134 138 4 794.24 0.41 331 135 138 3 817.42 0.86 332 135 139 4 800.66 2.23 333 136 139 3 819.60 0.23 334 136 140 4 800.64 2.25 335 137 140 3 821.75 0.84 336 137 141 4 819.63 1.19 337 138 141 3 822.72 0.36 338 138 142 4 820.94 0.53 339 139 142 3 820.94 0.53 340 140 143 3 823.82 0.23 341 141 146 5 777.12 8.32 342 141 147 6 777.12 8.32 343 142 147 5 776.96 8.25 344 143 146 3 827.62 2.17 345 143 147 4 807.67 0.85 346 144 147 3 801.73 2.12 347 148 159 11 704.78 18.67 348 148 181 33 455.36 1.81 349 148 182 34 455.32 1.80 350 148 200 52 369.88 0.94 351 149 190 41 406.71 1.42 352 149 194 45 406.35 1.51 353 149 195 46 406.17 1.45 354 149 196 47 401.03 2.09 355 149 197 48 393.36 0.69 356 149 230 81 282.45 0.68 357 150 154 4 782.38 6.07 358 150 155 5 776.84 8.70 359 150 159 9 704.89 18.62 360 150 200 50 376.19 1.90 361 150 230 80 282.24 0.64 362 151 154 3 800.22 2.11 363 151 155 4 782.38 6.07 364 151 230 79 282.47 0.69 365 151 231 80 282.19 0.67 366 151 232 81 279.91 0.49 367 152 155 3 815.66 4.87 368 152 156 4 782.38 6.07 369 153 156 3 819.81 6.95 370 153 157 4 782.23 6.00 371 153 158 5 782.21 6.02 372 153 232 79 281.53 0.93 373 153 233 80 278.19 0.55 374 153 234 81 265.99 1.68 375 154 157 3 821.99 5.86 376 154 158 4 776.83 8.68 377 154 159 5 772.53 10.71 378 154 160 6 772.53 10.71 379 154 200 46 380.01 1.33 380 154 228 74 283.06 0.67 381 154 230 76 282.47 0.69 382 154 234 80 253.31 1.35 383 155 159 4 776.51 8.71 384 155 160 5 722.46 9.87 385 157 160 3 812.34 1.64 386 157 161 4 799.14 8.22 387 157 236 79 221.16 0.92 388 158 161 3 815.66 2.73 389 158 162 4 802.97 6.32 390 158 228 70 283.04 0.69 391 158 234 76 252.84 1.13 392 158 235 77 251.97 1.10 393 158 236 78 217.36 0.97 394 158 237 79 178.01 18.68 395 158 238 80 177.75 18.58 396 158 241 83 139.30 0.58 397 159 162 3 815.66 2.73 398 159 163 4 802.97 6.32 399 159 180 21 455.65 1.84 400 159 200 41 370.19 0.95 401 159 201 42 370.13 0.93 402 159 202 43 369.95 1.02 403 159 228 69 282.99 0.66 404 160 163 3 810.83 2.37 405 160 164 4 803.30 6.14 406 161 164 3 812.84 1.21 407 161 165 4 793.10 1.04 408 161 236 75 229.34 5.00 409 161 238 77 178.00 18.70 410 162 165 3 803.97 2.83 411 162 166 4 799.12 4.05 412 162 202 40 369.87 1.02 413 162 224 62 284.08 0.45 414 162 226 64 283.43 0.66 415 162 238 76 177.87 18.64 416 163 166 3 824.45 4.60 417 163 167 4 808.48 8.73 418 163 202 39 369.83 1.03 419 164 167 3 812.80 10.43 420 164 168 4 808.47 8.74 421 165 168 3 830.30 2.16 422 165 169 4 818.61 3.67 423 165 222 57 284.52 0.34 424 165 224 59 284.11 0.47 425 165 238 73 178.00 18.69 426 165 239 74 177.93 18.67 427 166 169 3 818.62 3.67 428 166 170 4 818.61 3.67 429 166 176 10 455.93 1.82 430 166 178 12 455.87 1.81 431 166 204 38 354.62 1.07 432 167 170 3 815.56 3.81 433 167 171 4 809.61 0.83 434 168 171 3 807.60 3.31 435 168 172 4 799.67 4.14 436 169 172 3 807.09 2.27 437 169 173 4 796.97 2.79 438 169 174 5 762.19 5.90 439 169 176 7 691.69 3.73 440 169 206 37 352.16 1.24 441 169 222 53 284.50 0.34 442 169 224 55 284.40 0.33 443 170 173 3 799.71 4.16 444 170 174 4 773.62 0.18 445 170 176 6 761.25 6.36 446 173 210 37 342.31 1.80 447 174 206 32 352.06 1.19 448 174 210 36 329.00 0.96 449 175 206 31 351.89 1.17 450 175 207 32 351.57 1.24 451 175 210 35 287.85 0.02 452 175 211 36 287.86 0.02 453 176 204 28 352.25 1.20 454 176 205 29 352.12 1.24 455 176 206 30 351.84 1.19 456 177 204 27 352.20 1.22 457 177 205 28 351.90 1.17 458 177 207 30 351.79 1.17 459 178 202 24 365.49 0.05 460 178 203 25 355.18 0.81 461 178 204 26 352.11 1.24 462 179 201 22 369.61 0.99 463 179 202 23 365.75 0.33 464 179 203 24 355.18 0.81 465 179 256 77 56.76 1.71 466 180 201 21 369.58 1.00 467 180 202 22 361.55 2.35 468 181 200 19 369.76 0.96 469 181 201 20 369.61 0.99 470 182 197 15 389.49 1.17 471 182 199 17 370.37 1.02 472 182 200 18 369.78 0.96 473 182 201 19 369.76 0.96 474 183 197 14 392.81 0.47 475 183 198 15 391.50 0.17 476 183 199 16 378.23 2.27 477 183 201 18 369.92 0.96 478 183 251 68 85.80 0.69 479 183 252 69 85.06 0.34 480 184 195 11 406.30 1.50 481 184 197 13 396.51 0.10 482 184 198 14 392.82 0.46 483 185 195 10 406.28 1.49 484 185 196 11 406.04 1.51 485 185 198 13 394.03 0.97 486 186 196 10 406.26 1.49 487 186 197 11 406.18 1.46 488 186 198 12 404.43 1.01 489 186 230 44 282.66 0.67 490 189 192 3 786.60 0.50 491 189 193 4 674.43 14.29 492 189 194 5 406.44 1.51 493 189 195 6 406.35 1.51 494 190 193 3 807.27 0.88 495 190 194 4 639.94 20.05 496 190 196 6 406.35 1.51 497 196 230 34 282.53 0.67 498 197 200 3 849.10 2.30 499 197 230 33 282.39 0.59 500 198 229 31 282.76 0.72 501 198 230 32 282.59 0.69 502 199 227 28 283.12 0.68 503 199 228 29 283.03 0.66 504 199 229 30 282.62 0.66 505 199 230 31 282.40 0.59 506 200 227 27 283.10 0.68 507 200 228 28 282.84 0.74 508 201 226 25 283.12 0.68 509 201 227 26 282.99 0.68 510 201 248 47 87.92 0.49 511 201 252 51 85.11 0.35 512 201 256 55 80.08 2.26 513 202 225 23 283.40 0.59 514 202 226 24 283.12 0.68 515 202 248 46 87.95 0.51 516 203 223 20 284.03 0.45 517 203 224 21 283.92 0.47 518 203 225 22 283.22 0.68 519 203 245 42 87.98 0.58 520 203 248 45 87.97 0.57 521 203 256 53 57.18 1.88 522 204 223 19 284.04 0.45 523 204 224 20 283.74 0.56 524 205 217 12 285.68 0.69 525 205 221 16 284.42 0.39 526 205 222 17 284.14 0.45 527 205 223 18 284.03 0.47 528 205 257 52 55.53 1.40 529 206 221 15 284.41 0.39 530 206 222 16 284.14 0.45 531 207 212 5 286.66 0.70 532 207 213 6 286.53 0.70 533 207 216 9 285.88 0.68 534 207 217 10 285.81 0.67 535 207 221 14 284.50 0.34 536 208 216 8 286.00 0.72 537 208 217 9 285.90 0.68 538 208 218 10 285.88 0.68 539 208 221 13 284.51 0.34 540 209 212 3 819.89 4.66 541 212 215 3 826.57 4.31 542 212 216 4 443.01 79.14 543 213 216 3 806.91 7.72 544 214 217 3 817.27 2.54 545 214 259 45 54.84 1.53 546 214 260 46 51.95 0.12 547 217 221 4 637.78 14.62 548 217 260 43 51.98 0.14 549 217 264 47 51.58 0.20 550 217 268 51 48.85 0.12 551 218 221 3 794.55 5.06 552 218 264 46 51.58 0.20 553 218 268 50 48.86 0.12 554 219 264 45 51.58 0.20 555 219 267 48 49.40 0.14 556 219 268 49 48.86 0.12 557 221 268 47 48.83 0.12 558 222 273 51 20.98 0.56 559 223 245 22 88.11 0.57 560 223 268 45 47.32 0.83 561 223 273 50 21.72 0.29 562 224 238 14 177.84 18.63 563 224 273 49 20.65 0.39 564 225 242 17 112.70 0.89 565 225 243 18 101.48 0.60 566 225 244 19 94.25 2.25 567 225 245 20 88.17 0.55 568 225 248 23 88.16 0.55 569 226 238 12 177.55 18.48 570 226 241 15 128.02 3.12 571 226 242 16 116.40 0.96 572 226 243 17 112.69 0.90 573 226 248 22 94.17 2.25 574 227 241 14 129.88 4.00 575 227 243 16 121.73 5.40 576 227 248 21 88.17 0.54 577 227 251 24 87.89 0.54 578 228 234 6 635.64 23.55 579 228 238 10 177.54 18.48 580 228 241 13 132.12 2.88 581 229 234 5 674.90 28.06 582 229 241 12 137.46 0.24 583 231 234 3 850.10 0.79 584 234 241 7 711.82 7.91 585 235 240 5 725.41 14.70 586 235 241 6 725.41 14.70 587 237 240 3 837.39 2.12 588 237 241 4 820.41 0.00 589 238 241 3 820.41 0.00 590 238 242 4 805.08 2.23 591 239 242 3 814.97 2.72 592 242 272 30 32.34 0.67 593 242 273 31 27.59 1.71 594 243 247 4 804.81 1.70 595 243 248 5 798.40 4.17 596 243 272 29 32.58 0.60 597 244 247 3 814.94 3.36 598 244 248 4 805.88 7.89 599 244 269 25 47.33 0.84 600 244 270 26 47.11 0.87 601 244 271 27 44.20 0.31 602 244 272 28 32.04 0.82 603 245 248 3 807.22 7.22 604 245 249 4 798.49 4.17 605 245 256 11 70.35 2.58 606 245 269 24 47.16 0.89 607 246 249 3 805.90 7.87 608 246 250 4 798.49 4.17 609 246 269 23 47.40 0.81 610 246 270 24 47.14 0.89 611 247 250 3 802.98 1.92 612 247 251 4 798.49 4.17 613 248 251 3 799.31 3.76 614 248 252 4 798.49 4.17 615 248 256 8 375.06 145.25 616 249 252 3 815.60 2.32 617 249 253 4 726.71 6.23 618 249 254 5 688.20 20.66 619 249 255 6 633.11 21.95 620 249 256 7 375.08 145.23 621 249 269 20 47.40 0.81 622 252 256 4 880.70 0.78 623 255 258 3 838.09 4.40 624 255 265 10 383.83 145.55 625 256 269 13 47.08 0.85 626 257 260 3 799.16 3.85 627 257 265 8 656.82 15.97 628 257 268 11 361.27 156.94 629 257 269 12 47.30 0.86 630 258 265 7 691.26 30.26 631 260 264 4 789.34 0.27 632 260 265 5 735.31 21.08 633 260 268 8 721.23 15.71 634 261 264 3 807.41 0.43 635 261 265 4 788.90 0.05 636 262 265 3 789.34 0.27 637 262 266 4 788.95 0.07 638 263 266 3 795.75 2.94 639 263 267 4 789.34 0.27 640 264 267 3 814.50 1.93 641 264 268 4 790.90 0.89 642 265 268 3 819.46 0.09 643 265 269 4 768.08 4.76 644 266 270 4 767.72 5.96 645 267 270 3 843.36 1.78 646 267 271 4 801.41 6.29