BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 13 12 139.50 8.18 2 1 20 19 95.27 0.93 3 1 23 22 90.02 0.07 4 1 24 23 63.77 13.16 5 1 27 26 63.77 13.16 6 2 5 3 820.41 4.74 7 3 6 3 816.86 2.84 8 3 7 4 808.32 1.44 9 4 7 3 819.78 1.38 10 4 8 4 811.25 2.90 11 5 8 3 821.34 2.15 12 5 9 4 803.43 3.89 13 6 9 3 827.75 1.05 14 6 10 4 798.00 1.30 15 6 13 7 772.36 0.06 16 7 10 3 816.63 3.77 17 7 11 4 821.10 1.54 18 8 94 86 135.82 9.85 19 8 95 87 135.82 9.85 20 8 96 88 135.91 9.81 21 8 97 89 135.93 9.79 22 9 12 3 815.95 8.44 23 9 13 4 792.75 2.79 24 9 23 14 135.84 9.75 25 9 27 18 135.86 9.73 26 9 49 40 131.87 7.96 27 9 50 41 131.65 7.85 28 9 51 42 131.99 7.90 29 9 95 86 135.91 9.81 30 9 98 89 136.00 9.77 31 10 13 3 793.12 2.96 32 10 14 4 792.75 2.79 33 11 15 4 810.13 2.07 34 12 15 3 818.60 2.16 35 12 18 6 139.00 8.30 36 12 23 11 136.09 9.74 37 12 26 14 136.08 9.75 38 12 50 38 135.68 9.67 39 13 19 6 136.09 9.74 40 13 20 7 136.08 9.74 41 13 23 10 136.02 9.77 42 15 18 3 805.96 3.67 43 15 19 4 421.05 111.83 44 17 212 195 122.92 3.44 45 18 22 4 786.97 3.08 46 18 23 5 787.93 2.60 47 18 26 8 772.33 0.29 48 19 22 3 812.95 4.45 49 19 23 4 804.80 0.38 50 20 23 3 814.69 2.33 51 20 24 4 816.53 6.25 52 21 24 3 827.22 0.91 53 21 25 4 819.53 4.75 54 22 25 3 819.53 4.75 55 22 26 4 819.53 4.75 56 23 26 3 826.65 8.30 57 23 27 4 819.53 4.74 58 23 50 27 729.88 0.28 59 23 51 28 730.05 0.37 60 24 27 3 803.61 4.34 61 24 28 4 799.26 2.18 62 25 28 3 806.41 2.94 63 25 29 4 798.02 1.56 64 25 209 184 182.78 0.48 65 25 210 185 182.34 0.67 66 26 29 3 809.58 4.21 67 26 50 24 764.89 1.73 68 26 51 25 764.89 1.74 69 26 52 26 741.44 1.46 70 26 53 27 747.16 4.22 71 26 206 180 187.62 1.94 72 26 209 183 186.49 1.38 73 26 210 184 182.72 0.49 74 27 51 24 769.62 0.20 75 27 53 26 747.16 4.22 76 27 98 71 606.72 0.72 77 29 52 23 770.16 0.04 78 29 53 24 770.12 0.06 79 29 54 25 770.11 0.06 80 29 120 91 552.36 0.49 81 29 209 180 197.97 0.53 82 30 52 22 770.21 0.03 83 30 54 24 770.11 0.05 84 30 56 26 770.11 0.05 85 30 118 88 552.45 0.52 86 30 119 89 552.39 0.49 87 30 120 90 552.37 0.50 88 30 121 91 552.38 0.51 89 31 47 16 770.26 0.01 90 31 52 21 770.24 0.02 91 31 54 23 770.20 0.03 92 31 55 24 770.11 0.05 93 31 56 25 770.11 0.05 94 31 121 90 552.38 0.50 95 31 123 92 552.37 0.50 96 31 209 178 210.21 1.21 97 32 56 24 770.11 0.05 98 32 58 26 770.11 0.05 99 32 107 75 553.08 0.55 100 32 115 83 552.56 0.51 101 32 116 84 552.53 0.52 102 32 119 87 552.51 0.51 103 32 121 89 552.48 0.52 104 32 122 90 552.38 0.50 105 32 123 91 552.38 0.50 106 33 36 3 900.93 3.48 107 33 40 7 770.38 0.01 108 33 44 11 770.36 0.02 109 33 47 14 770.36 0.02 110 33 55 22 770.25 0.01 111 33 56 23 770.20 0.03 112 33 57 24 770.15 0.03 113 33 58 25 770.12 0.05 114 33 123 90 552.40 0.50 115 33 125 92 552.37 0.50 116 34 58 24 770.14 0.04 117 34 107 73 562.95 4.38 118 34 112 78 552.95 0.55 119 34 122 88 552.52 0.51 120 34 123 89 552.44 0.49 121 34 124 90 552.39 0.51 122 34 125 91 552.37 0.50 123 34 126 92 552.33 0.50 124 34 135 101 447.67 1.06 125 35 40 5 770.64 0.00 126 35 58 23 770.20 0.02 127 35 59 24 770.14 0.04 128 35 125 90 552.38 0.50 129 35 126 91 552.35 0.50 130 36 40 4 770.64 0.00 131 36 41 5 770.62 0.00 132 36 57 21 770.24 0.01 133 36 59 23 770.15 0.04 134 36 74 38 627.43 2.09 135 36 83 47 624.59 2.04 136 36 84 48 624.28 2.12 137 36 87 51 624.06 2.08 138 37 41 4 787.00 4.83 139 37 71 34 627.46 2.10 140 37 83 46 625.91 2.10 141 38 42 4 807.45 1.10 142 38 70 32 627.44 2.10 143 38 71 33 627.44 2.10 144 38 194 156 251.55 0.54 145 39 42 3 844.07 1.36 146 39 43 4 812.32 1.33 147 39 125 86 552.46 0.52 148 39 127 88 552.42 0.50 149 39 149 110 440.92 1.36 150 39 150 111 440.88 1.36 151 39 194 155 251.52 0.56 152 39 197 158 251.42 0.53 153 39 198 159 251.16 0.64 154 39 201 162 251.27 0.55 155 40 43 3 813.27 0.86 156 40 44 4 805.00 2.31 157 40 125 85 552.38 0.51 158 41 44 3 819.40 2.20 159 41 45 4 813.25 0.87 160 41 71 30 627.43 2.09 161 41 72 31 627.43 2.09 162 41 73 32 627.33 2.06 163 41 74 33 627.41 2.08 164 42 45 3 807.28 2.12 165 42 46 4 804.98 2.31 166 42 72 30 627.42 2.09 167 42 195 153 251.43 0.54 168 42 198 156 251.17 0.62 169 42 199 157 251.15 0.60 170 43 46 3 825.71 3.48 171 43 47 4 804.23 2.65 172 43 123 80 552.51 0.52 173 43 125 82 552.42 0.50 174 43 198 155 251.16 0.63 175 43 202 159 236.63 0.34 176 44 47 3 806.33 2.68 177 44 48 4 804.18 2.62 178 44 55 11 770.39 0.03 179 44 57 13 770.22 0.02 180 44 91 47 622.50 1.54 181 45 48 3 818.44 3.37 182 45 49 4 812.01 1.30 183 45 72 27 627.42 2.09 184 45 73 28 627.15 1.99 185 45 91 46 622.52 1.55 186 45 94 49 622.10 1.62 187 46 49 3 814.96 0.18 188 46 50 4 812.01 1.30 189 46 199 153 251.15 0.61 190 46 202 156 236.65 0.33 191 47 50 3 820.22 4.62 192 47 51 4 805.85 4.38 193 47 52 5 793.28 1.46 194 47 55 8 770.41 0.04 195 47 123 76 552.47 0.52 196 47 202 155 236.65 0.33 197 47 205 158 226.84 0.04 198 47 206 159 224.12 1.26 199 48 51 3 820.08 10.17 200 48 55 7 770.44 0.04 201 48 91 43 622.50 1.54 202 48 94 46 622.00 1.65 203 48 95 47 621.67 1.53 204 49 94 45 622.00 1.65 205 50 206 156 224.16 1.24 206 51 98 47 617.91 1.49 207 52 55 3 856.01 2.06 208 52 98 46 617.91 1.49 209 52 209 157 210.42 1.31 210 53 98 45 617.89 1.48 211 53 99 46 617.87 1.49 212 54 99 45 617.84 1.50 213 55 99 44 617.84 1.50 214 55 100 45 617.72 1.48 215 55 101 46 617.71 1.48 216 56 101 45 617.57 1.45 217 56 103 47 617.44 1.49 218 57 84 27 623.13 1.66 219 57 87 30 623.09 1.69 220 57 88 31 622.62 1.47 221 57 91 34 622.63 1.47 222 57 100 43 617.70 1.47 223 57 101 44 617.58 1.46 224 57 102 45 617.43 1.50 225 57 103 46 616.77 1.80 226 58 103 45 616.57 1.70 227 58 107 49 562.96 4.38 228 59 83 24 624.75 1.96 229 59 84 25 624.06 2.09 230 59 103 44 617.18 1.56 231 59 104 45 616.57 1.70 232 59 105 46 616.52 1.71 233 60 105 45 616.53 1.71 234 60 106 46 616.52 1.71 235 61 82 21 626.06 2.08 236 61 83 22 626.02 2.06 237 62 80 18 626.21 2.04 238 62 81 19 626.16 2.03 239 62 82 20 626.06 2.08 240 62 83 21 626.06 2.08 241 63 67 4 818.39 2.79 242 63 68 5 811.38 0.70 243 63 74 11 627.46 2.07 244 63 78 15 626.49 1.97 245 63 79 16 626.40 1.95 246 63 80 17 626.25 2.02 247 63 81 18 626.24 2.02 248 63 83 20 626.13 2.06 249 63 86 23 626.12 2.06 250 64 67 3 828.80 1.49 251 64 79 15 626.49 1.96 252 65 68 3 835.13 4.66 253 65 79 14 626.54 1.95 254 66 69 3 847.88 0.70 255 67 70 3 851.89 2.31 256 67 71 4 741.57 22.10 257 67 83 16 626.22 2.05 258 68 74 6 627.59 2.06 259 68 75 7 627.57 2.06 260 68 76 8 627.56 2.06 261 68 79 11 627.00 2.02 262 69 189 120 252.14 0.62 263 69 191 122 251.69 0.58 264 70 189 119 252.06 0.66 265 70 191 121 251.66 0.58 266 70 194 124 251.65 0.58 267 71 74 3 835.07 0.62 268 71 83 12 625.95 2.10 269 73 87 14 624.54 2.00 270 73 90 17 624.27 1.99 271 73 91 18 623.70 1.77 272 74 78 4 748.97 28.12 273 74 83 9 625.98 2.08 274 74 86 12 625.02 1.92 275 74 87 13 625.58 1.96 276 75 78 3 809.12 1.95 277 76 79 3 818.19 2.58 278 78 81 3 812.91 4.04 279 78 86 8 626.15 2.01 280 81 86 5 789.35 3.07 281 81 89 8 780.75 1.80 282 82 85 3 820.96 2.43 283 82 86 4 801.44 1.74 284 82 104 22 617.38 1.54 285 83 86 3 814.03 1.03 286 83 87 4 801.44 1.74 287 84 87 3 809.72 1.78 288 84 88 4 801.43 1.74 289 84 102 18 617.47 1.51 290 84 103 19 617.45 1.51 291 84 104 20 617.23 1.53 292 85 88 3 827.39 0.78 293 85 89 4 809.83 0.98 294 85 102 17 617.50 1.50 295 85 104 19 617.45 1.51 296 86 89 3 823.37 1.23 297 86 90 4 817.74 2.96 298 87 90 3 824.81 0.57 299 87 91 4 816.51 2.21 300 88 91 3 826.29 0.21 301 88 92 4 808.76 2.10 302 88 100 12 617.77 1.50 303 88 101 13 617.74 1.49 304 88 102 14 617.54 1.47 305 89 92 3 825.18 3.42 306 89 93 4 821.00 1.33 307 90 93 3 821.00 1.32 308 90 94 4 820.99 1.32 309 91 94 3 816.82 3.45 310 91 95 4 811.03 0.55 311 91 100 9 617.85 1.50 312 92 95 3 807.99 0.10 313 92 96 4 730.84 25.68 314 92 100 8 617.89 1.49 315 95 98 3 838.46 3.43 316 103 107 4 844.95 1.89 317 107 111 4 799.76 2.39 318 107 112 5 661.43 6.75 319 107 115 8 636.77 2.50 320 108 111 3 820.92 2.46 321 108 112 4 789.40 1.11 322 108 134 26 447.77 1.07 323 109 112 3 836.96 3.01 324 109 113 4 807.23 5.60 325 110 113 3 807.26 5.58 326 110 114 4 804.16 4.04 327 111 114 3 850.00 2.72 328 111 115 4 804.80 3.57 329 112 115 3 818.64 2.39 330 112 116 4 804.80 3.58 331 112 134 22 447.73 1.06 332 112 135 23 447.69 1.09 333 112 138 26 447.44 0.97 334 113 116 3 817.05 1.59 335 113 117 4 801.05 1.67 336 113 138 25 447.43 0.97 337 113 142 29 446.93 1.02 338 114 117 3 806.25 0.90 339 114 118 4 801.03 1.68 340 115 118 3 824.98 0.67 341 115 119 4 795.44 1.12 342 116 119 3 791.06 2.06 343 116 122 6 552.54 0.49 344 116 138 22 447.31 0.94 345 116 139 23 447.18 0.94 346 116 142 26 446.81 0.97 347 116 144 28 446.50 0.94 348 117 142 25 446.81 0.96 349 119 122 3 837.61 3.35 350 119 144 25 446.49 0.93 351 120 144 24 446.48 0.95 352 120 145 25 446.46 0.94 353 120 209 89 210.21 1.21 354 121 144 23 446.48 0.95 355 121 145 24 446.44 0.95 356 121 147 26 446.14 0.96 357 121 205 84 227.13 0.18 358 121 208 87 216.77 1.72 359 121 209 88 212.57 0.24 360 122 135 13 447.50 1.02 361 122 139 17 447.06 0.88 362 122 144 22 446.51 0.94 363 122 145 23 446.46 0.96 364 122 146 24 446.28 0.92 365 122 147 25 446.19 0.94 366 123 147 24 444.68 0.24 367 123 201 78 250.28 0.62 368 123 205 82 233.18 1.05 369 124 132 8 447.62 1.02 370 124 135 11 447.55 1.02 371 124 146 22 446.40 0.95 372 124 147 23 446.28 0.92 373 124 148 24 442.30 0.70 374 124 149 25 442.30 0.70 375 124 201 77 250.56 0.77 376 125 201 76 250.69 0.83 377 126 131 5 788.13 3.40 378 126 132 6 676.76 9.94 379 126 135 9 651.66 12.68 380 127 132 5 801.88 0.40 381 127 150 23 441.41 1.15 382 127 151 24 441.04 1.33 383 128 131 3 826.44 3.33 384 128 132 4 804.19 1.55 385 129 132 3 816.40 1.82 386 129 133 4 810.57 4.74 387 129 170 41 361.18 1.98 388 129 174 45 358.13 1.93 389 129 177 48 270.22 5.94 390 129 178 49 268.51 6.78 391 129 179 50 256.77 1.26 392 129 180 51 256.79 1.27 393 130 133 3 817.07 1.50 394 130 134 4 812.27 3.89 395 130 177 47 268.55 6.77 396 131 134 3 823.37 1.65 397 131 135 4 805.46 0.49 398 132 135 3 818.97 5.71 399 132 136 4 799.89 2.46 400 132 148 16 446.47 0.96 401 132 149 17 446.43 0.95 402 132 150 18 446.39 0.95 403 132 170 38 361.12 1.96 404 132 182 50 256.64 1.25 405 133 136 3 816.49 1.65 406 133 137 4 803.29 4.26 407 133 170 37 361.12 1.96 408 133 171 38 360.93 2.02 409 133 174 41 357.00 1.37 410 133 176 43 339.83 0.26 411 133 177 44 268.55 6.77 412 134 137 3 803.28 4.26 413 134 138 4 803.28 4.26 414 135 138 3 823.32 1.80 415 135 139 4 803.22 4.29 416 135 146 11 446.69 1.01 417 136 139 3 815.79 1.97 418 136 140 4 803.22 4.29 419 136 146 10 446.70 1.00 420 136 161 25 363.01 1.25 421 136 169 33 361.11 1.95 422 136 170 34 361.04 1.93 423 136 171 35 360.68 1.92 424 137 140 3 820.50 2.56 425 137 141 4 804.98 5.19 426 138 141 3 804.46 5.46 427 138 142 4 798.41 8.48 428 139 143 4 746.27 32.61 429 139 144 5 446.83 0.99 430 139 146 7 446.67 1.00 431 139 165 26 362.55 1.38 432 140 143 3 796.47 7.51 433 140 165 25 362.53 1.37 434 140 169 29 361.10 1.95 435 140 242 102 21.52 2.54 436 140 243 103 21.52 2.53 437 143 163 20 362.50 1.37 438 143 165 22 362.50 1.37 439 144 165 21 362.51 1.38 440 145 161 16 362.56 1.36 441 145 162 17 362.53 1.37 442 145 163 18 362.50 1.38 443 145 165 20 362.50 1.39 444 145 208 63 224.07 1.18 445 146 160 14 362.77 1.32 446 146 161 15 362.56 1.36 447 147 158 11 363.12 1.21 448 147 159 12 362.98 1.23 449 147 160 13 362.77 1.32 450 147 201 54 250.56 0.77 451 147 204 57 234.08 0.93 452 147 205 58 233.81 0.91 453 147 208 61 230.87 0.10 454 148 158 10 363.12 1.21 455 148 159 11 363.00 1.22 456 148 170 22 360.91 2.00 457 148 201 53 250.94 0.71 458 149 157 8 363.15 1.20 459 149 158 9 363.11 1.21 460 149 197 48 251.50 0.56 461 149 201 52 251.36 0.58 462 150 156 6 363.27 1.20 463 150 157 7 363.21 1.22 464 150 182 32 253.50 0.41 465 150 197 47 251.51 0.57 466 151 155 4 367.87 3.43 467 151 156 5 363.27 1.20 468 151 189 38 252.26 0.62 469 151 194 43 251.63 0.56 470 152 155 3 826.02 2.87 471 152 182 30 254.02 0.10 472 152 185 33 252.56 0.60 473 152 186 34 252.46 0.61 474 152 189 37 252.27 0.62 475 153 188 35 252.34 0.61 476 153 189 36 252.27 0.62 477 153 190 37 252.27 0.62 478 154 190 36 252.25 0.62 479 154 193 39 251.96 0.58 480 154 223 69 143.09 16.46 481 154 224 70 141.60 16.74 482 154 225 71 141.59 16.75 483 155 193 38 251.86 0.61 484 155 222 67 144.40 15.89 485 156 193 37 251.68 0.58 486 156 197 41 251.66 0.57 487 156 221 65 144.47 15.93 488 156 222 66 144.24 15.84 489 157 197 40 251.62 0.56 490 157 219 62 149.48 17.75 491 157 220 63 146.24 16.34 492 157 221 64 144.30 15.84 493 158 200 42 251.42 0.56 494 158 201 43 251.41 0.57 495 158 204 46 251.03 0.69 496 158 219 61 146.52 16.33 497 158 220 62 144.50 15.90 498 159 170 11 361.11 1.95 499 159 173 14 360.38 2.02 500 159 218 59 152.35 16.33 501 159 219 60 145.62 16.04 502 159 234 75 76.23 0.30 503 160 204 44 244.42 1.17 504 160 208 48 233.22 1.05 505 160 216 56 154.49 15.51 506 160 217 57 154.35 15.45 507 161 165 4 583.26 60.15 508 161 168 7 361.18 1.99 509 161 169 8 361.03 1.93 510 161 170 9 360.85 1.84 511 161 234 73 75.91 0.16 512 162 165 3 813.87 6.89 513 162 208 46 230.87 0.10 514 162 216 54 174.41 6.24 515 165 168 3 741.87 22.97 516 165 236 71 74.95 0.47 517 165 243 78 21.75 2.42 518 166 236 70 75.09 0.41 519 167 217 50 174.19 6.79 520 167 234 67 75.53 0.35 521 167 235 68 75.09 0.41 522 167 236 69 75.04 0.39 523 168 172 4 727.29 27.62 524 168 236 68 74.42 0.20 525 168 243 75 24.05 1.28 526 169 172 3 774.22 17.55 527 169 173 4 774.22 17.55 528 169 240 71 41.38 0.66 529 169 242 73 32.81 1.38 530 169 243 74 21.79 2.40 531 170 173 3 814.55 1.92 532 170 174 4 743.40 19.59 533 171 174 3 797.94 2.02 534 171 175 4 727.38 27.58 535 171 240 69 41.06 0.81 536 171 241 70 41.06 0.81 537 171 242 71 34.54 2.15 538 172 236 64 75.20 0.35 539 172 237 65 74.19 0.08 540 172 240 68 41.24 0.72 541 172 243 71 33.53 1.70 542 173 234 61 79.35 1.33 543 174 178 4 845.12 4.15 544 174 180 6 273.99 4.04 545 179 182 3 879.77 1.22 546 180 183 3 879.12 1.54 547 182 186 4 796.16 1.17 548 183 186 3 826.18 3.27 549 184 187 3 862.68 1.34 550 185 188 3 890.63 0.85 551 189 194 5 734.59 31.31 552 190 193 3 823.64 4.49 553 190 194 4 800.02 1.39 554 191 194 3 833.56 1.31 555 191 195 4 749.43 23.92 556 192 195 3 814.94 8.79 557 192 196 4 804.03 10.91 558 193 196 3 831.93 2.71 559 193 197 4 831.60 2.87 560 193 222 29 143.14 16.43 561 193 224 31 134.28 20.32 562 194 197 3 831.61 2.87 563 194 198 4 817.50 4.17 564 195 198 3 832.30 2.52 565 195 199 4 803.35 2.91 566 196 199 3 823.59 1.83 567 196 200 4 811.35 1.10 568 196 229 33 80.61 1.53 569 197 200 3 825.70 0.78 570 197 201 4 810.45 1.56 571 197 222 25 143.07 16.41 572 197 229 32 80.88 1.52 573 198 201 3 810.53 1.60 574 198 202 4 815.26 0.79 575 199 202 3 825.21 0.54 576 199 203 4 815.27 0.78 577 200 203 3 824.81 5.55 578 200 204 4 815.23 0.76 579 200 220 20 144.49 15.89 580 200 229 29 80.58 1.54 581 201 204 3 816.70 1.49 582 201 205 4 815.25 0.78 583 202 205 3 815.25 0.79 584 202 206 4 809.18 3.82 585 203 206 3 812.31 2.25 586 203 207 4 809.18 3.81 587 204 207 3 812.30 2.25 588 204 208 4 809.17 3.82 589 204 214 10 195.50 4.33 590 204 220 16 144.51 15.89 591 205 208 3 810.36 4.42 592 205 209 4 794.48 0.60 593 206 209 3 820.91 6.64 594 206 210 4 740.31 26.48 595 207 210 3 810.12 1.05 596 207 211 4 733.33 29.98 597 207 212 5 476.66 89.75 598 207 214 7 250.65 23.25 599 208 211 3 789.01 3.90 600 217 234 17 75.44 0.29 601 218 231 13 79.84 1.46 602 218 232 14 79.56 1.32 603 218 234 16 76.23 0.30 604 219 231 12 79.84 1.46 605 219 232 13 79.54 1.33 606 219 234 15 76.25 0.31 607 220 230 10 80.26 1.43 608 220 231 11 79.84 1.46 609 221 229 8 80.91 1.53 610 221 230 9 80.24 1.44 611 221 232 11 80.09 1.40 612 222 227 5 263.02 1.72 613 222 228 6 82.30 1.93 614 222 229 7 80.86 1.50 615 223 226 3 828.00 4.17 616 223 227 4 363.93 48.73 617 223 228 5 82.41 1.98 618 224 227 3 722.91 28.11 619 236 243 7 31.02 2.28 620 236 244 8 26.55 0.04 621 237 240 3 810.31 5.27 622 240 243 3 670.52 1.18