BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 2 218 216 106.87 0.76 2 2 219 217 106.83 0.78 3 2 220 218 106.75 0.77 4 2 228 226 52.66 2.16 5 2 232 230 40.25 1.14 6 3 31 28 317.45 98.98 7 3 218 215 106.95 0.79 8 3 219 216 106.87 0.77 9 3 220 217 106.87 0.76 10 3 232 229 54.30 0.15 11 4 29 25 319.38 98.15 12 4 30 26 318.72 98.40 13 4 31 27 317.50 98.98 14 4 32 28 317.48 98.98 15 4 220 216 106.88 0.77 16 4 222 218 106.81 0.78 17 4 225 221 77.48 0.43 18 4 228 224 73.89 0.32 19 4 229 225 71.31 0.24 20 4 232 228 57.72 0.96 21 5 32 27 317.56 98.96 22 5 34 29 318.74 98.34 23 5 106 101 317.47 98.97 24 5 198 193 107.59 0.84 25 5 219 214 107.59 0.84 26 5 220 215 106.92 0.78 27 5 221 216 106.87 0.77 28 5 222 217 106.86 0.76 29 6 9 3 825.37 4.47 30 6 10 4 816.80 0.18 31 6 25 19 319.46 98.11 32 6 30 24 319.38 98.14 33 6 32 26 319.30 98.14 34 6 33 27 318.81 98.37 35 6 34 28 318.87 98.32 36 6 222 216 106.89 0.77 37 6 225 219 87.40 0.73 38 7 11 4 849.77 0.27 39 7 34 27 319.42 98.08 40 7 200 193 107.59 0.83 41 7 221 214 107.55 0.82 42 7 222 215 107.11 0.81 43 7 223 216 106.91 0.78 44 8 11 3 866.65 2.31 45 8 16 8 319.94 97.81 46 8 17 9 319.67 97.95 47 8 34 26 319.69 97.95 48 8 36 28 319.66 97.94 49 8 49 41 319.76 97.91 50 8 55 47 565.54 3.60 51 9 21 12 319.94 97.82 52 9 22 13 319.93 97.84 53 9 25 16 320.22 97.98 54 10 13 3 855.83 1.65 55 10 14 4 787.83 1.41 56 10 21 11 320.12 97.89 57 10 24 14 460.46 27.95 58 10 25 15 320.19 97.95 59 10 225 215 106.38 0.83 60 11 14 3 821.20 2.67 61 11 16 5 569.47 26.80 62 11 80 69 620.39 0.30 63 11 223 212 107.31 0.90 64 12 80 68 621.37 0.53 65 12 223 211 107.41 0.85 66 12 224 212 107.17 0.83 67 13 24 11 621.66 0.60 68 13 80 67 621.41 0.54 69 13 84 71 621.32 0.58 70 13 224 211 107.17 0.83 71 14 20 6 622.72 1.07 72 14 21 7 622.52 1.02 73 14 24 10 622.52 1.02 74 14 80 66 621.43 0.54 75 14 84 70 621.32 0.58 76 15 18 3 853.15 3.15 77 15 21 6 708.57 14.53 78 15 76 61 621.25 0.59 79 15 77 62 621.26 0.60 80 15 79 64 621.45 0.53 81 15 80 65 621.45 0.53 82 15 84 69 621.40 0.54 83 16 21 5 753.69 4.33 84 16 49 33 621.50 0.58 85 16 55 39 621.52 0.54 86 16 56 40 621.46 0.53 87 16 79 63 621.53 0.54 88 17 21 4 809.06 1.37 89 17 35 18 621.50 0.58 90 17 36 19 621.50 0.58 91 17 47 30 621.32 0.60 92 17 48 31 621.36 0.62 93 17 49 32 621.52 0.57 94 17 90 73 619.92 0.39 95 18 21 3 843.35 0.33 96 18 22 4 819.46 3.52 97 18 87 69 620.88 0.26 98 18 90 72 618.77 0.10 99 18 95 77 573.33 0.47 100 19 22 3 841.51 1.25 101 19 23 4 820.75 2.85 102 19 88 69 620.70 0.23 103 19 90 71 618.88 0.15 104 19 94 75 575.18 1.38 105 19 95 76 573.33 0.46 106 19 98 79 549.71 1.19 107 20 23 3 843.77 3.25 108 20 24 4 827.32 0.42 109 20 84 64 621.45 0.53 110 21 24 3 843.77 3.25 111 21 25 4 827.32 0.43 112 22 25 3 838.53 5.87 113 22 26 4 822.10 3.05 114 22 33 11 621.59 0.58 115 22 95 73 573.36 0.48 116 23 26 3 822.95 3.47 117 23 27 4 822.10 3.05 118 23 98 75 549.69 1.18 119 24 27 3 828.36 0.77 120 24 28 4 822.10 3.04 121 25 28 3 827.88 5.92 122 25 29 4 821.82 2.89 123 25 30 5 783.61 10.89 124 25 33 8 622.99 0.03 125 25 225 200 82.39 2.00 126 26 29 3 832.56 8.26 127 26 30 4 783.72 10.84 128 26 33 7 705.78 15.21 129 26 99 73 549.57 1.23 130 26 102 76 504.35 20.33 131 26 103 77 504.29 20.32 132 28 225 197 82.40 2.00 133 28 226 198 82.14 1.90 134 28 229 201 73.97 0.33 135 29 232 203 63.36 0.57 136 30 33 3 885.38 1.43 137 30 225 195 81.37 1.51 138 31 103 72 504.30 20.33 139 31 104 73 504.29 20.33 140 31 232 201 59.16 0.30 141 32 104 72 504.25 20.32 142 32 105 73 504.20 20.31 143 32 106 74 504.17 20.29 144 32 129 97 361.32 29.41 145 32 131 99 361.13 29.42 146 32 151 119 222.52 9.21 147 32 219 187 107.66 0.82 148 33 95 62 573.47 0.53 149 33 99 66 554.37 1.16 150 33 103 70 504.31 20.32 151 33 104 71 504.25 20.32 152 33 105 72 503.85 20.18 153 33 106 73 503.84 20.18 154 34 106 72 502.57 20.39 155 34 108 74 502.46 20.44 156 35 47 12 700.28 16.56 157 35 92 57 619.36 1.10 158 35 95 60 573.50 0.52 159 35 96 61 573.47 0.52 160 35 99 64 558.98 2.23 161 35 105 70 503.81 20.17 162 35 106 71 502.48 20.43 163 35 107 72 502.17 20.57 164 35 108 73 501.17 20.88 165 36 46 10 700.83 16.47 166 36 47 11 700.28 16.56 167 36 48 12 700.27 16.56 168 36 49 13 699.81 16.79 169 36 108 72 498.61 21.05 170 36 110 74 498.25 21.17 171 37 45 8 701.15 16.34 172 37 46 9 700.83 16.47 173 37 92 55 621.62 0.40 174 37 107 70 502.01 20.66 175 37 108 71 501.17 20.88 176 37 109 72 498.56 21.04 177 37 110 73 498.49 21.05 178 37 111 74 498.23 21.17 179 37 112 75 497.98 21.13 180 38 45 7 701.27 16.41 181 38 46 8 700.86 16.47 182 38 48 10 700.78 16.47 183 38 49 11 700.74 16.48 184 38 50 12 700.73 16.48 185 38 51 13 700.17 16.69 186 38 63 25 699.48 16.99 187 38 110 72 498.31 21.12 188 38 111 73 498.16 21.18 189 38 112 74 497.97 21.14 190 39 43 4 707.59 13.28 191 39 44 5 701.41 16.37 192 39 45 6 701.30 16.41 193 39 63 24 699.50 17.00 194 39 64 25 699.43 16.97 195 39 111 72 498.18 21.18 196 39 112 73 497.97 21.14 197 39 113 74 497.97 21.14 198 39 116 77 483.49 21.32 199 40 43 3 826.72 5.79 200 40 44 4 701.79 16.18 201 40 46 6 701.32 16.41 202 40 63 23 699.51 17.00 203 40 64 24 699.44 16.96 204 40 66 26 699.29 16.98 205 40 68 28 698.08 16.71 206 40 71 31 692.26 14.10 207 41 63 22 699.58 16.98 208 41 64 23 699.44 16.96 209 41 65 24 699.40 16.96 210 41 66 25 699.33 16.99 211 41 67 26 699.20 16.94 212 42 67 25 699.30 16.99 213 42 68 26 699.19 16.93 214 43 116 73 490.56 22.25 215 44 68 24 698.39 16.55 216 44 91 47 622.35 0.27 217 45 91 46 622.01 0.39 218 45 92 47 621.86 0.41 219 45 112 67 498.03 21.16 220 45 116 71 476.95 20.53 221 45 117 72 441.81 10.70 222 45 120 75 418.72 7.41 223 46 68 22 697.08 16.21 224 46 71 25 689.74 15.25 225 46 72 26 691.85 14.18 226 46 89 43 622.47 0.21 227 46 90 44 622.03 0.40 228 46 91 45 621.81 0.42 229 46 92 46 621.73 0.39 230 47 89 42 622.04 0.41 231 47 90 43 621.97 0.41 232 47 92 45 621.62 0.40 233 47 95 48 573.48 0.52 234 48 53 5 778.75 3.23 235 48 56 8 700.94 17.19 236 48 57 9 700.95 17.18 237 48 63 15 700.21 17.30 238 48 89 41 622.03 0.41 239 49 110 61 498.31 21.12 240 50 55 5 731.73 1.95 241 51 62 11 700.39 17.36 242 51 63 12 700.38 17.36 243 52 61 9 700.75 17.19 244 52 62 10 700.40 17.36 245 52 63 11 700.39 17.36 246 53 56 3 857.34 1.97 247 53 57 4 750.59 7.37 248 53 59 6 700.98 17.24 249 53 60 7 700.97 17.24 250 53 61 8 700.96 17.24 251 53 63 10 700.40 17.36 252 53 66 13 700.32 17.32 253 53 74 21 684.86 14.12 254 54 57 3 847.80 0.24 255 54 59 5 701.01 17.23 256 54 60 6 700.96 17.24 257 54 61 7 700.97 17.24 258 56 76 20 667.15 5.72 259 56 79 23 635.75 3.29 260 57 74 17 670.54 6.96 261 57 75 18 670.41 7.01 262 57 76 19 669.92 7.09 263 57 79 22 640.45 0.96 264 57 89 32 623.15 0.18 265 58 74 16 670.56 6.97 266 58 75 17 670.53 6.97 267 59 74 15 686.11 13.56 268 62 65 3 826.70 3.37 269 62 66 4 819.13 0.29 270 63 66 3 825.89 9.18 271 63 71 8 722.23 6.85 272 66 70 4 827.40 5.26 273 66 71 5 806.43 2.61 274 66 74 8 786.95 4.08 275 67 70 3 839.93 0.99 276 67 71 4 832.88 2.52 277 68 71 3 836.82 0.55 278 68 72 4 820.33 3.77 279 68 91 23 622.90 0.10 280 69 72 3 841.13 2.71 281 69 73 4 835.88 1.02 282 70 73 3 835.88 1.01 283 70 74 4 823.16 5.35 284 71 74 3 830.29 1.79 285 71 89 18 623.02 0.10 286 72 91 19 622.99 0.10 287 74 89 15 623.23 0.14 288 75 78 3 873.72 1.94 289 75 84 9 623.82 0.14 290 75 85 10 623.63 0.11 291 75 86 11 623.45 0.09 292 75 87 12 623.39 0.12 293 76 79 3 899.41 0.41 294 76 87 11 623.39 0.12 295 76 88 12 623.36 0.12 296 76 89 13 623.36 0.12 297 77 80 3 849.92 3.24 298 77 81 4 820.34 1.77 299 77 84 7 623.77 0.11 300 77 87 10 623.40 0.12 301 78 81 3 832.50 0.21 302 78 84 6 646.18 11.05 303 78 85 7 623.71 0.14 304 80 84 4 821.74 0.90 305 81 84 3 852.80 4.59 306 81 85 4 817.52 1.82 307 82 85 3 826.72 4.34 308 84 87 3 842.22 1.76 309 90 95 5 803.41 5.76 310 91 94 3 837.79 4.87 311 91 95 4 822.24 1.12 312 92 95 3 837.32 6.74 313 92 96 4 818.93 0.53 314 92 120 28 363.82 28.79 315 93 96 3 858.80 1.07 316 93 97 4 832.89 5.38 317 93 120 27 363.82 28.80 318 94 97 3 832.90 5.38 319 94 98 4 827.15 2.51 320 95 98 3 838.82 3.32 321 95 99 4 827.14 2.51 322 96 99 3 824.46 0.05 323 96 100 4 819.25 0.63 324 96 105 9 504.15 20.29 325 96 107 11 503.82 20.13 326 96 120 24 363.82 28.79 327 96 121 25 363.69 28.75 328 96 124 28 362.53 29.19 329 96 126 30 362.31 29.23 330 97 100 3 820.68 0.59 331 97 124 27 362.53 29.19 332 98 102 4 824.16 1.15 333 99 102 3 824.16 1.15 334 99 103 4 790.93 2.98 335 99 105 6 601.58 68.95 336 100 124 24 362.52 29.19 337 100 126 26 362.10 29.25 338 104 129 25 361.17 29.41 339 105 126 21 362.13 29.17 340 105 129 24 361.32 29.41 341 105 130 25 361.14 29.42 342 105 131 26 361.14 29.42 343 106 131 25 361.13 29.42 344 106 132 26 361.08 29.40 345 106 133 27 360.88 29.50 346 106 153 47 222.29 9.13 347 106 198 92 172.72 14.48 348 106 221 115 107.59 0.83 349 107 117 10 419.46 1.03 350 107 121 14 363.69 28.71 351 107 130 23 361.32 29.41 352 107 131 24 361.16 29.43 353 107 132 25 360.86 29.51 354 107 133 26 360.86 29.51 355 108 133 25 360.83 29.52 356 108 134 26 360.74 29.48 357 108 135 27 360.81 29.52 358 109 112 3 881.54 1.33 359 109 114 5 784.89 5.76 360 109 117 8 554.57 59.41 361 109 133 24 360.88 29.52 362 109 134 25 360.81 29.52 363 109 139 30 339.21 28.56 364 109 143 34 317.67 27.13 365 110 136 26 351.45 33.82 366 110 139 29 343.81 30.88 367 111 138 27 350.60 34.24 368 111 139 28 344.47 31.21 369 112 117 5 827.09 1.63 370 112 139 27 349.52 33.71 371 113 116 3 840.14 0.30 372 113 117 4 827.10 1.63 373 114 117 3 830.28 2.01 374 114 118 4 827.10 1.63 375 114 139 25 344.50 31.20 376 114 142 28 317.50 24.47 377 114 143 29 314.91 25.77 378 114 146 32 308.28 24.42 379 115 118 3 830.28 2.01 380 115 119 4 822.31 0.74 381 115 146 31 266.17 3.39 382 116 119 3 831.02 2.39 383 116 120 4 822.30 0.74 384 117 120 3 831.25 1.74 385 117 121 4 824.30 1.74 386 117 132 15 361.80 29.39 387 118 121 3 824.34 1.77 388 118 122 4 827.30 0.29 389 118 146 28 264.98 3.45 390 118 147 29 226.90 9.85 391 118 150 32 226.02 10.13 392 119 122 3 833.36 3.32 393 119 123 4 827.30 0.29 394 120 123 3 835.50 3.81 395 120 124 4 825.46 0.65 396 121 124 3 831.81 1.96 397 121 125 4 825.43 0.64 398 121 126 5 771.72 5.04 399 121 127 6 751.80 2.30 400 121 130 9 361.97 29.34 401 121 132 11 361.81 29.39 402 121 150 29 225.55 10.37 403 122 125 3 827.81 0.53 404 122 127 5 768.20 4.16 405 122 149 27 226.03 10.13 406 122 150 28 225.99 10.15 407 126 129 3 836.37 0.11 408 126 130 4 831.03 2.56 409 127 130 3 842.98 1.75 410 127 150 23 222.87 9.25 411 128 151 23 222.75 9.27 412 129 151 22 222.71 9.28 413 130 151 21 222.51 9.21 414 131 151 20 222.46 9.19 415 131 152 21 222.44 9.19 416 131 153 22 222.34 9.14 417 131 173 42 222.03 9.29 418 131 198 67 220.38 9.25 419 132 143 11 305.98 21.45 420 132 150 18 222.72 9.27 421 132 151 19 222.47 9.19 422 132 152 20 222.16 9.05 423 132 153 21 222.16 9.07 424 133 153 20 222.03 9.01 425 133 154 21 222.00 9.01 426 133 155 22 222.00 9.01 427 134 139 5 786.58 3.85 428 134 140 6 727.58 6.50 429 135 140 5 802.35 9.15 430 136 139 3 805.16 4.95 431 136 140 4 813.00 3.83 432 137 140 3 827.91 2.69 433 137 141 4 802.41 9.12 434 138 141 3 846.67 1.45 435 138 142 4 830.17 4.75 436 139 142 3 831.98 5.65 437 139 143 4 828.49 5.60 438 140 143 3 840.91 0.61 439 140 144 4 828.49 5.59 440 141 144 3 821.75 3.60 441 141 145 4 819.50 4.73 442 142 145 3 840.77 0.45 443 142 146 4 814.55 0.01 444 143 146 3 819.97 0.84 445 143 147 4 795.88 6.42 446 143 150 7 775.69 9.46 447 143 152 9 515.46 134.57 448 144 148 4 787.90 3.39 449 144 152 8 515.58 134.58 450 144 170 26 258.17 24.65 451 145 148 3 839.90 0.52 452 147 150 3 843.05 1.05 453 147 152 5 840.32 0.31 454 148 152 4 867.46 1.59 455 151 173 22 326.94 28.71 456 151 196 45 326.94 28.71 457 151 219 68 136.53 10.21 458 152 170 18 327.56 28.69 459 152 171 19 327.23 28.71 460 152 172 20 327.07 28.75 461 152 173 21 326.95 28.71 462 153 171 18 327.38 28.75 463 153 173 20 326.98 28.72 464 153 175 22 327.12 28.79 465 153 198 45 326.94 28.71 466 154 158 4 818.71 1.42 467 154 159 5 755.04 4.53 468 154 165 11 327.58 28.69 469 154 169 15 327.68 28.70 470 154 171 17 327.58 28.68 471 154 173 19 327.37 28.76 472 154 174 20 327.28 28.73 473 154 175 21 327.27 28.73 474 155 158 3 830.82 4.63 475 155 159 4 809.84 1.50 476 155 175 20 327.29 28.73 477 156 159 3 825.48 1.97 478 156 160 4 771.44 5.57 479 156 161 5 757.00 3.55 480 156 162 6 745.21 6.90 481 156 174 18 327.62 28.70 482 156 175 19 327.57 28.72 483 156 176 20 327.38 28.77 484 156 180 24 327.57 28.71 485 156 186 30 327.65 28.71 486 157 160 3 845.80 4.78 487 157 180 23 327.60 28.71 488 158 169 11 327.88 28.71 489 159 164 5 392.10 60.72 490 159 165 6 327.77 28.75 491 159 169 10 327.86 28.71 492 160 164 4 807.70 2.46 493 161 164 3 813.94 3.60 494 161 165 4 816.46 1.97 495 162 165 3 841.11 3.69 496 162 174 12 327.87 28.80 497 162 189 27 328.05 28.79 498 162 190 28 327.94 28.81 499 162 195 33 327.94 28.80 500 163 167 4 803.44 4.52 501 164 167 3 808.99 0.67 502 164 168 4 692.38 21.16 503 164 169 5 666.72 8.33 504 165 169 4 515.10 64.53 505 165 170 5 515.10 64.53 506 165 171 6 328.22 28.82 507 165 173 8 328.01 28.79 508 165 174 9 327.79 28.83 509 165 195 30 328.15 28.78 510 166 170 4 328.28 28.86 511 166 171 5 328.28 28.86 512 171 195 24 425.04 44.04 513 172 195 23 425.04 44.04 514 172 196 24 425.05 44.04 515 173 196 23 425.05 44.04 516 173 198 25 425.06 44.05 517 174 190 16 425.20 44.05 518 174 195 21 425.17 44.05 519 174 196 22 425.12 44.07 520 174 197 23 425.09 44.05 521 174 198 24 425.07 44.06 522 175 198 23 425.08 44.06 523 175 200 25 425.06 44.05 524 175 221 46 135.65 10.42 525 176 180 4 787.63 3.00 526 176 181 5 787.30 2.84 527 176 186 10 425.46 44.06 528 176 197 21 425.20 44.06 529 176 198 22 425.13 44.07 530 176 199 23 425.07 44.06 531 176 200 24 425.06 44.06 532 177 180 3 830.49 3.99 533 177 181 4 803.08 4.70 534 177 199 22 425.10 44.07 535 178 181 3 824.44 0.96 536 178 182 4 808.59 1.18 537 178 199 21 425.19 44.05 538 178 200 22 425.13 44.07 539 178 201 23 425.08 44.06 540 178 208 30 140.56 10.29 541 179 182 3 829.40 3.15 542 179 183 4 811.86 0.65 543 180 183 3 822.79 0.15 544 180 186 6 623.79 54.42 545 181 187 6 425.49 44.06 546 181 199 18 425.22 44.05 547 181 211 30 140.55 10.29 548 181 212 31 140.43 10.34 549 181 215 34 137.24 10.25 550 181 217 36 137.12 10.25 551 182 211 29 140.55 10.29 552 183 186 3 827.86 7.44 553 184 188 4 825.83 6.40 554 185 188 3 825.83 6.40 555 185 189 4 792.45 0.66 556 186 189 3 827.14 5.74 557 186 190 4 796.72 1.47 558 187 190 3 843.27 2.31 559 187 191 4 812.12 3.31 560 187 197 10 425.49 43.98 561 187 215 28 137.69 10.53 562 187 216 29 137.10 10.25 563 187 217 30 137.10 10.25 564 188 191 3 821.88 1.56 565 188 192 4 813.68 2.53 566 189 192 3 819.69 5.53 567 189 193 4 813.69 2.53 568 190 193 3 824.78 3.02 569 190 194 4 810.11 4.32 570 190 195 5 782.45 2.64 571 190 197 7 603.07 44.63 572 191 194 3 819.95 1.23 573 191 195 4 800.24 6.24 574 191 197 6 764.84 11.45 575 191 216 25 137.09 10.25 576 191 218 27 137.00 10.29 577 196 218 22 136.57 10.20 578 196 219 23 136.56 10.20 579 197 216 19 137.08 10.24 580 197 217 20 136.75 10.22 581 197 218 21 136.59 10.21 582 197 219 22 136.55 10.21 583 198 217 19 136.76 10.23 584 198 219 21 136.49 10.23 585 198 221 23 135.84 10.34 586 199 212 13 137.75 10.52 587 199 217 18 136.87 10.27 588 199 219 20 136.53 10.21 589 199 220 21 136.12 10.20 590 199 221 22 135.67 10.42 591 200 221 21 135.61 10.44 592 201 205 4 901.30 3.64 593 201 208 7 249.61 43.90 594 201 209 8 140.56 10.29 595 201 212 11 140.53 10.30 596 201 220 19 136.41 10.22 597 201 221 20 135.99 10.26 598 202 222 20 135.02 10.70 599 202 223 21 134.98 10.68 600 203 209 6 805.99 5.00 601 204 209 5 806.00 5.00 602 204 224 20 135.05 10.67 603 204 227 23 134.65 10.70 604 205 208 3 841.69 2.18 605 205 209 4 835.01 5.51 606 206 209 3 838.86 3.60 607 206 210 4 831.02 7.52 608 206 227 21 134.71 10.67 609 206 230 24 134.09 10.96 610 206 231 25 115.64 19.59 611 207 210 3 835.38 5.34 612 207 211 4 819.14 1.58 613 208 211 3 832.52 3.91 614 208 212 4 828.13 6.10 615 209 212 3 835.10 3.44 616 209 213 4 813.85 0.97 617 209 220 11 136.85 10.23 618 209 222 13 136.78 10.23 619 209 227 18 134.67 10.67 620 209 228 19 116.89 19.45 621 209 231 22 110.70 20.73 622 210 213 3 828.19 6.08 623 210 214 4 813.87 0.96 624 210 231 21 108.75 21.71 625 211 214 3 826.75 2.07 626 211 215 4 813.87 0.96 627 212 215 3 835.86 2.52 628 212 217 5 776.44 19.64 629 212 220 8 136.86 10.23 630 212 228 16 116.90 19.46 631 213 228 15 115.41 20.21 632 213 231 18 101.83 21.03 633 213 235 22 51.13 0.15 634 214 235 21 52.43 0.78 635 220 228 8 115.01 20.21 636 222 225 3 889.35 0.89 637 222 228 6 802.95 5.63 638 224 227 3 841.93 3.91 639 224 228 4 832.17 0.21 640 225 228 3 841.75 3.99 641 225 229 4 820.98 3.07 642 226 229 3 835.94 0.42 643 226 230 4 832.63 0.59 644 227 230 3 833.27 0.92 645 227 231 4 832.63 0.59 646 228 231 3 833.27 0.92 647 228 232 4 832.61 0.60 648 229 232 3 831.61 0.09 649 229 233 4 831.32 0.01 650 230 233 3 834.63 1.61 651 230 234 4 831.34 0.01 652 231 234 3 834.58 1.64 653 231 235 4 823.79 3.79 654 232 235 3 831.54 0.09 655 232 236 4 823.77 3.79 656 233 236 3 831.54 0.10 657 233 237 4 823.87 3.27 658 234 237 3 840.59 2.50 659 234 238 4 830.93 0.04 660 235 238 3 839.52 3.03 661 235 239 4 819.05 1.93 662 236 239 3 830.54 0.15 663 236 240 4 819.05 1.93 664 237 240 3 831.24 0.38