BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 27 26 590.36 1.79 2 1 47 46 547.87 0.97 3 1 48 47 547.86 0.97 4 2 27 25 590.38 1.80 5 2 29 27 590.00 1.76 6 2 44 42 548.03 0.96 7 2 45 43 547.99 0.97 8 2 46 44 547.88 0.97 9 2 47 45 547.88 0.97 10 2 48 46 547.87 0.97 11 3 20 17 590.54 1.75 12 3 26 23 590.56 1.76 13 3 27 24 590.53 1.76 14 3 28 25 590.45 1.74 15 3 29 26 590.36 1.79 16 3 46 43 547.98 0.97 17 3 48 45 547.88 0.97 18 3 50 47 547.87 0.97 19 3 225 222 80.05 0.19 20 4 29 25 590.48 1.76 21 4 30 26 590.00 1.76 22 4 41 37 548.10 0.96 23 4 46 42 548.03 0.96 24 4 48 44 547.94 0.98 25 4 49 45 547.87 0.97 26 4 50 46 547.87 0.97 27 5 8 3 840.96 3.71 28 5 13 8 591.44 1.51 29 5 16 11 591.24 1.52 30 5 17 12 591.11 1.48 31 5 20 15 591.19 1.51 32 5 28 23 590.56 1.76 33 5 30 25 590.50 1.77 34 5 31 26 590.32 1.78 35 5 32 27 590.27 1.80 36 5 49 44 547.94 0.98 37 5 50 45 547.89 0.97 38 6 32 26 590.30 1.81 39 6 38 32 548.50 0.97 40 6 41 35 548.33 0.96 41 6 49 43 548.02 0.96 42 6 50 44 547.95 0.98 43 6 51 45 547.88 0.97 44 6 52 46 547.87 0.97 45 6 53 47 547.75 0.96 46 6 61 55 449.62 0.71 47 6 65 59 449.59 0.70 48 7 10 3 827.93 1.19 49 7 13 6 591.53 1.54 50 7 32 25 590.48 1.76 51 7 33 26 590.22 1.79 52 7 52 45 547.89 0.97 53 7 53 46 547.66 0.92 54 8 13 5 591.66 1.59 55 8 14 6 591.54 1.53 56 8 17 9 591.54 1.54 57 8 31 23 590.54 1.76 58 8 32 24 590.52 1.77 59 8 33 25 590.45 1.75 60 9 33 24 590.52 1.77 61 10 13 3 807.60 4.51 62 10 14 4 802.72 6.41 63 10 33 23 590.54 1.76 64 11 15 4 813.46 2.88 65 11 214 203 88.00 0.90 66 12 15 3 836.17 3.62 67 12 16 4 816.13 4.21 68 12 52 40 547.95 0.98 69 12 214 202 87.98 0.89 70 13 16 3 836.40 4.17 71 13 17 4 827.49 1.46 72 13 52 39 547.90 0.97 73 14 17 3 832.44 1.01 74 14 18 4 827.49 1.46 75 15 18 3 848.57 3.90 76 15 19 4 827.15 1.66 77 15 214 199 87.92 0.85 78 15 215 200 87.86 0.84 79 15 218 203 86.20 0.12 80 16 19 3 839.77 4.65 81 16 20 4 831.27 0.41 82 16 50 34 547.95 0.98 83 16 52 36 547.90 0.97 84 16 217 201 86.23 0.13 85 16 218 202 86.20 0.12 86 16 221 205 85.66 0.10 87 17 20 3 839.77 4.65 88 17 21 4 831.28 0.42 89 17 28 11 590.70 1.72 90 17 31 14 590.54 1.76 91 18 21 3 837.33 2.79 92 18 22 4 823.56 4.29 93 19 22 3 838.68 3.46 94 19 23 4 823.56 4.29 95 19 218 199 86.19 0.12 96 19 222 203 85.54 0.07 97 20 23 3 848.52 1.47 98 20 26 6 590.82 1.70 99 20 27 7 590.76 1.72 100 20 28 8 590.68 1.73 101 20 50 30 547.89 0.97 102 20 222 202 85.30 0.01 103 23 26 3 853.11 0.66 104 23 222 199 85.55 0.07 105 23 226 203 80.09 0.19 106 25 226 201 80.08 0.19 107 26 222 196 85.27 0.01 108 26 225 199 80.08 0.18 109 26 226 200 79.97 0.21 110 28 31 3 945.49 4.58 111 29 44 15 548.01 0.97 112 29 46 17 547.94 0.98 113 30 41 11 548.10 0.96 114 30 46 16 548.03 0.96 115 32 36 4 612.36 6.71 116 32 37 5 610.03 7.87 117 32 38 6 572.38 10.96 118 32 40 8 548.41 0.93 119 32 41 9 548.34 0.96 120 33 36 3 843.41 5.90 121 36 40 4 839.30 3.81 122 37 40 3 847.15 0.10 123 38 41 3 843.05 2.02 124 38 61 23 449.62 0.71 125 38 64 26 449.59 0.71 126 39 42 3 833.19 6.71 127 39 43 4 811.42 1.38 128 39 64 25 449.59 0.72 129 40 43 3 819.14 2.70 130 40 44 4 811.21 1.27 131 41 44 3 815.70 0.96 132 41 45 4 809.79 1.98 133 41 46 5 758.82 13.70 134 41 49 8 547.97 0.96 135 41 64 23 449.57 0.71 136 41 65 24 449.32 0.69 137 41 70 29 448.38 0.92 138 42 45 3 814.50 5.78 139 42 64 22 449.54 0.70 140 42 68 26 448.43 0.92 141 42 70 28 448.36 0.92 142 46 49 3 906.78 1.89 143 46 70 24 448.32 0.94 144 47 70 23 448.31 0.93 145 47 71 24 448.31 0.93 146 48 70 22 448.35 0.94 147 48 71 23 448.31 0.93 148 48 73 25 448.11 0.91 149 48 225 177 80.06 0.20 150 48 228 180 79.03 0.04 151 49 65 16 448.65 1.01 152 49 70 21 448.38 0.94 153 49 71 22 448.35 0.94 154 49 72 23 448.13 0.92 155 49 73 24 448.11 0.91 156 50 73 23 448.12 0.90 157 50 221 171 85.67 0.12 158 50 225 175 80.08 0.19 159 51 58 7 449.75 0.78 160 51 61 10 449.62 0.71 161 51 73 22 448.28 0.96 162 51 74 23 448.14 0.92 163 51 75 24 448.11 0.91 164 51 76 25 447.94 0.96 165 52 75 23 448.14 0.92 166 52 76 24 447.92 0.96 167 52 105 53 238.35 0.51 168 52 221 169 86.14 0.10 169 53 57 4 821.49 3.97 170 53 58 5 697.54 31.91 171 53 61 8 604.87 78.22 172 53 76 23 448.09 0.91 173 54 57 3 842.15 4.98 174 54 58 4 784.73 2.85 175 54 76 22 448.20 0.93 176 55 58 3 847.01 2.54 177 55 76 21 448.25 0.95 178 55 77 22 448.17 0.93 179 55 78 23 448.01 0.92 180 55 83 28 400.31 2.19 181 57 60 3 846.79 0.07 182 57 61 4 824.01 4.73 183 58 61 3 826.33 2.66 184 58 62 4 818.79 6.07 185 58 74 16 448.38 0.95 186 58 76 18 448.19 0.96 187 58 79 21 447.84 0.96 188 58 83 25 397.23 1.39 189 59 62 3 827.02 3.00 190 59 63 4 821.72 5.67 191 59 79 20 446.99 1.39 192 59 83 24 397.01 1.41 193 59 86 27 259.85 4.49 194 59 87 28 251.35 0.25 195 59 90 31 251.34 0.24 196 60 63 3 832.77 2.03 197 60 64 4 830.89 1.10 198 61 64 3 832.77 2.03 199 61 65 4 832.76 2.04 200 62 65 3 838.35 4.82 201 62 66 4 827.72 2.68 202 62 72 10 448.44 0.92 203 62 74 12 448.38 0.94 204 62 79 17 443.87 0.43 205 62 87 25 251.09 0.21 206 62 90 28 250.93 0.26 207 62 99 37 249.69 0.07 208 62 101 39 249.50 0.09 209 63 66 3 834.21 0.56 210 63 67 4 827.49 1.83 211 63 90 27 250.93 0.26 212 64 67 3 830.44 1.27 213 64 68 4 827.41 1.79 214 64 70 6 790.99 11.12 215 65 68 3 828.42 2.28 216 65 69 4 818.69 2.58 217 65 70 5 763.72 15.38 218 65 72 7 448.45 0.93 219 66 69 3 828.96 2.01 220 66 87 21 251.06 0.20 221 66 90 24 250.91 0.27 222 66 91 25 250.91 0.27 223 66 94 28 250.46 0.05 224 66 97 31 250.06 0.01 225 66 99 33 249.59 0.04 226 67 94 27 250.43 0.03 227 69 94 25 250.27 0.01 228 69 97 28 249.62 0.04 229 70 97 27 249.62 0.04 230 71 96 25 250.11 0.00 231 71 97 26 249.69 0.07 232 71 98 27 249.48 0.04 233 71 228 157 79.08 0.01 234 71 229 158 78.28 0.34 235 72 98 26 249.69 0.07 236 72 99 27 249.22 0.09 237 72 100 28 249.05 0.07 238 72 228 156 79.78 0.30 239 73 100 27 248.77 0.17 240 73 105 32 229.19 0.18 241 73 221 148 85.75 0.09 242 73 224 151 85.16 0.04 243 73 225 152 84.48 0.22 244 73 228 155 79.73 0.33 245 74 79 5 846.48 2.55 246 74 99 25 249.61 0.11 247 74 100 26 249.31 0.14 248 74 101 27 248.69 0.17 249 74 102 28 248.68 0.17 250 74 105 31 241.13 1.98 251 75 102 27 248.58 0.21 252 75 105 30 228.90 0.34 253 76 79 3 1023.67 3.79 254 78 83 5 832.02 0.70 255 79 83 4 830.66 0.03 256 79 84 5 823.93 3.76 257 79 101 22 249.74 0.14 258 80 83 3 835.62 2.50 259 80 84 4 835.59 2.48 260 81 84 3 837.60 1.52 261 81 85 4 837.57 1.50 262 82 85 3 837.57 1.50 263 82 86 4 837.57 1.50 264 83 86 3 835.89 2.33 265 83 87 4 835.89 2.33 266 84 87 3 833.36 1.06 267 84 88 4 823.86 3.69 268 84 101 17 250.24 0.07 269 84 234 150 65.22 0.40 270 84 236 152 60.18 0.14 271 85 88 3 829.57 0.83 272 85 89 4 826.64 0.64 273 86 89 3 829.18 0.63 274 86 90 4 827.99 1.29 275 87 90 3 831.86 1.45 276 87 91 4 831.87 1.45 277 87 99 12 250.31 0.03 278 87 101 14 250.20 0.05 279 87 234 147 60.21 0.12 280 88 91 3 840.24 2.74 281 88 92 4 832.83 0.96 282 88 234 146 63.34 1.42 283 89 92 3 839.57 2.41 284 90 93 3 856.75 1.84 285 90 94 4 841.98 5.54 286 91 94 3 841.98 5.54 287 91 95 4 818.98 5.96 288 91 97 6 250.52 0.07 289 91 99 8 250.33 0.03 290 91 233 142 60.21 0.12 291 91 234 143 60.21 0.12 292 94 97 3 838.48 2.56 293 95 232 137 63.22 1.39 294 97 233 136 60.06 0.10 295 98 228 130 79.86 0.26 296 98 229 131 79.18 0.01 297 98 231 133 68.83 0.44 298 98 232 134 60.18 0.12 299 98 233 135 60.09 0.09 300 99 228 129 79.91 0.25 301 99 233 134 60.16 0.12 302 99 234 135 59.00 0.07 303 99 235 136 58.79 0.18 304 100 105 5 855.10 0.15 305 100 224 124 85.49 0.10 306 100 228 128 79.99 0.22 307 100 235 135 57.37 0.24 308 100 237 137 52.26 0.65 309 100 241 141 36.67 0.11 310 101 234 133 61.74 1.19 311 101 235 134 59.02 0.06 312 101 236 135 56.84 0.49 313 101 237 136 53.05 0.39 314 102 105 3 857.62 0.66 315 102 237 135 53.19 0.18 316 102 238 136 52.20 0.68 317 103 146 43 133.17 0.28 318 103 237 134 55.91 0.96 319 104 109 5 772.12 5.92 320 104 110 6 754.79 2.31 321 104 113 9 771.60 6.15 322 104 143 39 129.63 2.03 323 105 217 112 88.14 0.76 324 105 220 115 86.31 0.07 325 105 221 116 87.39 0.58 326 105 224 119 86.42 0.10 327 105 238 133 51.88 0.67 328 106 109 3 828.31 0.27 329 106 110 4 823.90 1.94 330 106 216 110 88.19 0.73 331 106 217 111 88.19 0.74 332 107 110 3 825.37 1.21 333 107 111 4 820.66 0.32 334 107 219 112 88.30 0.72 335 107 220 113 88.25 0.71 336 107 223 116 88.27 0.70 337 107 238 131 52.20 0.68 338 107 242 135 42.06 2.27 339 108 111 3 840.57 9.54 340 108 112 4 822.22 0.37 341 108 209 101 124.92 3.15 342 109 112 3 839.39 4.09 343 109 113 4 822.23 0.38 344 109 209 100 124.92 3.15 345 110 113 3 838.03 4.78 346 110 114 4 825.16 1.84 347 110 238 128 52.42 0.67 348 110 239 129 52.20 0.68 349 110 242 132 50.88 0.99 350 111 114 3 830.71 0.93 351 111 115 4 825.15 2.54 352 111 242 131 50.88 1.00 353 111 244 133 46.49 0.23 354 112 115 3 826.51 1.87 355 112 116 4 822.24 4.02 356 112 207 95 135.54 0.97 357 112 209 97 130.25 1.72 358 113 116 3 825.06 2.61 359 113 117 4 825.06 2.61 360 113 143 30 139.25 2.44 361 113 146 33 139.97 2.09 362 113 147 34 138.84 2.25 363 113 150 37 147.20 0.04 364 114 117 3 843.04 5.87 365 114 118 4 824.88 2.71 366 114 244 130 50.21 1.01 367 115 118 3 826.77 1.71 368 115 119 4 824.83 2.68 369 115 207 92 136.04 0.83 370 116 119 3 831.16 0.50 371 116 120 4 821.20 4.47 372 116 147 31 143.69 0.67 373 116 151 35 170.41 9.29 374 116 196 80 139.24 2.15 375 116 198 82 159.65 0.37 376 116 207 91 136.06 0.83 377 117 120 3 848.84 5.28 378 117 150 33 145.97 0.23 379 117 154 37 371.70 46.06 380 118 122 4 876.45 2.76 381 119 122 3 876.45 2.76 382 119 123 4 841.52 1.61 383 119 198 79 205.69 23.39 384 119 205 86 182.84 3.03 385 120 123 3 876.45 2.76 386 120 151 31 498.26 60.56 387 120 154 34 634.12 1.09 388 120 155 35 634.12 1.09 389 120 198 78 279.01 0.36 390 121 154 33 655.84 2.15 391 121 155 34 651.53 0.01 392 121 158 37 721.46 0.73 393 122 200 78 352.56 0.79 394 123 155 32 721.87 0.77 395 123 198 75 330.00 3.93 396 123 199 76 352.50 0.80 397 123 200 77 352.56 0.78 398 123 205 82 346.31 1.23 399 124 155 31 721.99 0.77 400 124 161 37 721.88 0.70 401 124 162 38 721.94 0.72 402 124 199 75 352.72 0.71 403 124 200 76 352.66 0.74 404 125 155 30 722.13 0.82 405 125 161 36 721.91 0.70 406 125 162 37 721.96 0.72 407 125 198 73 352.79 0.70 408 125 199 74 352.75 0.71 409 126 151 25 721.90 0.76 410 126 155 29 722.16 0.83 411 126 161 35 721.99 0.73 412 126 180 54 721.75 0.79 413 126 181 55 721.74 0.78 414 126 197 71 352.91 0.68 415 126 198 72 352.85 0.67 416 127 173 46 721.81 0.79 417 127 180 53 721.79 0.78 418 127 181 54 721.74 0.80 419 127 183 56 721.72 0.79 420 127 196 69 353.19 0.71 421 127 197 70 352.94 0.66 422 128 144 16 721.88 0.77 423 128 147 19 722.00 0.77 424 128 148 20 721.94 0.75 425 128 151 23 722.17 0.83 426 128 180 52 721.90 0.77 427 128 181 53 721.83 0.79 428 128 182 54 721.72 0.79 429 128 183 55 721.72 0.79 430 128 194 66 353.80 0.82 431 128 195 67 353.58 0.86 432 128 196 68 353.20 0.71 433 129 183 54 721.72 0.79 434 129 185 56 721.64 0.77 435 129 194 65 353.82 0.81 436 129 195 66 353.61 0.86 437 129 197 68 353.38 0.76 438 130 140 10 721.88 0.77 439 130 141 11 721.96 0.77 440 130 144 14 722.01 0.76 441 130 182 52 721.90 0.78 442 130 183 53 721.83 0.79 443 130 184 54 721.66 0.78 444 130 185 55 721.65 0.78 445 130 186 56 721.66 0.78 446 130 193 63 353.95 0.84 447 130 194 64 353.82 0.81 448 131 185 54 721.66 0.77 449 131 190 59 354.54 0.92 450 131 192 61 353.99 0.85 451 131 193 62 353.96 0.84 452 132 136 4 815.75 4.95 453 132 141 9 722.06 0.78 454 132 185 53 721.84 0.78 455 132 186 54 721.68 0.77 456 132 187 55 721.63 0.78 457 132 190 58 354.93 0.97 458 132 191 59 354.32 0.83 459 132 192 60 354.05 0.84 460 133 136 3 867.35 3.95 461 133 186 53 721.83 0.78 462 133 187 54 721.64 0.78 463 133 190 57 601.96 0.76 464 133 191 58 362.57 3.10 465 134 186 52 721.84 0.78 466 134 187 53 721.82 0.78 467 134 188 54 721.72 0.79 468 136 141 5 761.28 6.75 469 136 186 50 721.87 0.75 470 138 186 48 721.96 0.76 471 139 184 45 721.95 0.76 472 139 186 47 721.89 0.79 473 140 144 4 792.11 0.17 474 140 145 5 787.69 1.53 475 140 175 35 722.18 0.69 476 140 177 37 722.22 0.71 477 140 182 42 722.06 0.75 478 140 184 44 721.87 0.80 479 141 144 3 837.10 9.29 480 141 145 4 817.38 0.64 481 142 145 3 844.47 5.61 482 142 146 4 817.38 0.64 483 143 146 3 837.22 9.23 484 143 147 4 817.35 0.64 485 143 194 51 353.95 0.83 486 144 147 3 823.03 2.19 487 144 148 4 818.30 0.15 488 144 182 38 722.02 0.75 489 144 194 50 353.91 0.83 490 145 148 3 831.12 6.55 491 145 149 4 817.88 0.07 492 145 177 32 722.21 0.68 493 146 149 3 841.09 1.57 494 146 150 4 819.95 0.46 495 147 150 3 841.09 1.56 496 147 151 4 819.97 0.47 497 147 194 47 353.88 0.83 498 147 196 49 353.56 0.89 499 148 151 3 841.08 1.56 500 148 152 4 819.97 0.47 501 148 177 29 722.21 0.68 502 148 180 32 722.15 0.70 503 148 182 34 721.85 0.79 504 149 152 3 828.93 4.42 505 149 153 4 819.41 0.73 506 149 177 28 722.21 0.68 507 149 178 29 722.21 0.68 508 150 153 3 826.67 1.84 509 150 154 4 819.39 0.72 510 151 154 3 842.74 0.72 511 151 155 4 819.39 0.72 512 151 180 29 722.03 0.74 513 151 196 45 353.22 0.72 514 151 198 47 353.08 0.77 515 152 155 3 833.67 0.93 516 152 156 4 819.38 0.73 517 152 178 26 722.16 0.70 518 152 180 28 721.88 0.78 519 153 156 3 833.64 0.92 520 155 158 3 834.58 3.50 521 155 161 6 733.37 2.90 522 155 180 25 722.07 0.69 523 161 180 19 721.83 0.82 524 161 181 20 721.83 0.82 525 163 173 10 722.46 0.55 526 165 168 3 891.65 1.83 527 165 173 8 723.21 0.18 528 169 172 3 854.34 6.21 529 171 174 3 819.14 6.42 530 172 183 11 723.63 0.53 531 173 181 8 723.75 0.51 532 173 183 10 723.65 0.53 533 174 182 8 723.73 0.51 534 174 183 9 723.61 0.51 535 175 180 5 762.18 1.53 536 175 181 6 724.02 0.43 537 175 182 7 723.75 0.49 538 176 179 3 834.67 3.50 539 176 180 4 762.18 1.53 540 177 180 3 829.47 6.11 541 177 182 5 744.52 10.35 542 185 190 5 820.16 2.42 543 187 190 3 848.28 2.27 544 190 193 3 930.43 0.96 545 191 211 20 352.65 0.83 546 191 212 21 352.48 0.77 547 192 210 18 352.68 0.82 548 192 211 19 352.52 0.79 549 193 208 15 352.81 0.77 550 193 209 16 352.76 0.78 551 193 210 17 352.53 0.80 552 194 208 14 352.68 0.82 553 194 209 15 352.62 0.82 554 195 206 11 352.81 0.78 555 195 207 12 352.68 0.82 556 195 208 13 352.55 0.79 557 196 206 10 352.69 0.81 558 196 207 11 352.67 0.81 559 197 205 8 352.81 0.80 560 197 206 9 352.67 0.84 561 198 204 6 352.85 0.81 562 198 205 7 352.74 0.84 563 198 207 9 352.70 0.85 564 199 203 4 754.28 14.09 565 199 204 5 352.80 0.83 566 200 203 3 842.91 6.42 567 211 216 5 567.25 107.19 568 213 216 3 850.90 7.54 569 214 217 3 857.40 4.29 570 215 218 3 842.11 2.46 571 215 219 4 847.18 2.62 572 216 219 3 898.79 1.36 573 217 220 3 851.62 3.10 574 217 221 4 836.40 0.29 575 218 221 3 842.13 2.44 576 218 222 4 826.11 5.45 577 219 222 3 820.89 2.58 578 219 223 4 819.75 2.27 579 220 223 3 830.83 2.07 580 220 224 4 812.89 1.17 581 220 238 18 51.04 0.94 582 221 224 3 821.00 2.29 583 221 225 4 812.56 0.98 584 222 225 3 818.92 1.26 585 222 226 4 815.46 0.48 586 223 226 3 839.46 1.41 587 223 227 4 826.52 5.05 588 223 238 15 51.02 0.94 589 223 241 18 32.17 0.18 590 223 242 19 31.23 0.64 591 224 227 3 830.12 3.27 592 224 228 4 802.50 2.81 593 224 238 14 50.55 0.83 594 224 241 17 32.06 0.23 595 225 228 3 837.79 7.10 596 227 230 3 832.31 2.18 597 227 241 14 32.06 0.23 598 228 231 3 853.82 0.27 599 231 235 4 827.90 6.79 600 231 240 9 100.95 2.30 601 231 241 10 93.59 1.04 602 235 240 5 760.24 10.09 603 237 240 3 893.88 2.60 604 238 241 3 845.59 1.56 605 238 242 4 832.58 2.25 606 239 242 3 839.52 0.29