BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 1 27 26 745.41 2.34 2 1 28 27 709.83 1.82 3 2 72 70 622.07 0.34 4 3 27 24 748.05 1.39 5 3 28 25 748.05 1.39 6 3 72 69 622.05 0.34 7 3 121 118 412.82 1.52 8 3 220 217 69.59 0.29 9 4 28 24 748.10 1.36 10 4 68 64 622.19 0.38 11 4 69 65 622.22 0.37 12 4 70 66 622.18 0.36 13 4 71 67 622.09 0.35 14 4 72 68 622.07 0.35 15 4 73 69 622.07 0.35 16 5 22 17 748.53 1.26 17 5 27 22 748.48 1.23 18 5 28 23 748.41 1.26 19 5 29 24 748.07 1.37 20 5 30 25 748.08 1.36 21 5 68 63 622.37 0.39 22 5 71 66 622.17 0.37 23 5 73 68 622.07 0.35 24 6 30 24 748.10 1.36 25 6 32 26 748.02 1.39 26 6 48 42 686.13 2.46 27 6 60 54 622.58 0.33 28 6 61 55 622.40 0.25 29 6 64 58 622.54 0.33 30 6 71 65 622.23 0.36 31 6 73 67 622.16 0.36 32 6 74 68 622.07 0.35 33 6 75 69 622.05 0.34 34 7 10 3 796.64 5.86 35 7 15 8 749.13 1.21 36 7 18 11 748.83 1.19 37 7 19 12 748.75 1.16 38 7 22 15 748.78 1.18 39 7 29 22 748.53 1.26 40 7 30 23 748.42 1.26 41 7 31 24 748.10 1.35 42 7 32 25 748.07 1.37 43 7 75 68 622.06 0.34 44 7 77 70 622.04 0.34 45 8 32 24 748.33 1.24 46 8 52 44 627.94 1.47 47 8 60 52 623.03 0.40 48 8 75 67 622.16 0.36 49 8 76 68 622.07 0.35 50 8 77 69 622.05 0.34 51 8 78 70 620.95 0.07 52 8 108 100 429.85 2.98 53 9 12 3 837.94 1.62 54 9 15 6 749.25 1.21 55 9 32 23 748.41 1.26 56 9 33 24 748.09 1.35 57 9 77 68 622.07 0.35 58 9 78 69 621.09 0.10 59 10 15 5 749.25 1.21 60 10 16 6 749.31 1.24 61 10 31 21 748.51 1.26 62 10 32 22 748.48 1.24 63 11 16 5 750.72 0.88 64 11 31 20 748.56 1.26 65 11 33 22 748.41 1.24 66 11 35 24 748.29 1.26 67 11 36 25 709.26 6.96 68 11 39 28 687.30 2.59 69 12 15 3 825.69 8.12 70 12 16 4 779.82 6.48 71 12 180 168 186.63 1.43 72 12 181 169 186.64 1.42 73 13 16 3 807.80 1.83 74 13 17 4 788.29 2.48 75 13 179 166 186.63 1.43 76 13 181 168 186.64 1.42 77 13 207 194 94.86 2.57 78 14 17 3 799.60 3.17 79 14 18 4 789.83 1.73 80 14 174 160 263.89 0.74 81 14 177 163 189.00 1.19 82 14 179 165 186.63 1.43 83 14 207 193 94.86 2.58 84 14 210 196 94.73 2.54 85 15 18 3 795.33 1.02 86 15 19 4 792.35 0.48 87 15 77 62 622.01 0.34 88 16 19 3 795.33 1.02 89 16 20 4 792.34 0.47 90 16 39 23 687.29 2.59 91 16 42 26 687.14 2.54 92 16 43 27 686.97 2.50 93 17 20 3 800.44 1.54 94 17 21 4 792.34 0.47 95 17 207 190 94.85 2.57 96 17 208 191 94.82 2.56 97 17 211 194 93.28 2.75 98 18 21 3 806.51 1.49 99 18 22 4 788.19 2.55 100 18 75 57 622.07 0.35 101 18 127 109 411.72 1.32 102 18 172 154 272.61 0.33 103 18 211 193 93.28 2.75 104 18 214 196 90.92 3.85 105 19 22 3 792.93 0.12 106 19 23 4 779.86 6.42 107 19 29 10 748.64 1.25 108 19 31 12 748.49 1.25 109 19 43 24 686.84 2.48 110 20 23 3 793.26 0.05 111 20 24 4 780.19 6.58 112 20 43 23 686.84 2.48 113 21 24 3 780.19 6.58 114 21 25 4 780.18 6.58 115 21 211 190 93.29 2.75 116 21 215 194 90.89 3.87 117 22 25 3 807.56 1.10 118 22 26 4 780.09 6.62 119 22 27 5 765.60 4.96 120 22 29 7 749.65 1.19 121 22 73 51 622.13 0.38 122 22 75 53 622.06 0.34 123 22 215 193 90.88 3.86 124 22 218 196 90.42 3.87 125 23 26 3 780.03 6.66 126 23 27 4 773.36 3.58 127 23 29 6 749.13 1.45 128 23 43 20 686.82 2.48 129 25 215 190 90.89 3.87 130 25 219 194 90.42 3.88 131 25 221 196 90.37 3.89 132 27 73 46 622.07 0.35 133 27 218 191 90.42 3.88 134 27 219 192 90.35 3.90 135 28 68 40 622.21 0.37 136 29 45 16 686.28 2.49 137 29 46 17 686.26 2.48 138 29 68 39 622.34 0.41 139 30 46 16 686.26 2.48 140 30 48 18 686.16 2.46 141 30 68 38 622.36 0.40 142 31 36 5 687.35 2.56 143 31 39 8 687.25 2.58 144 31 40 9 686.93 2.43 145 31 43 12 686.83 2.43 146 31 45 14 686.51 2.41 147 31 46 15 686.34 2.49 148 31 47 16 686.25 2.48 149 31 48 17 686.18 2.47 150 32 48 16 686.15 2.46 151 32 60 28 623.00 0.41 152 33 36 3 802.71 6.78 153 33 48 15 686.30 2.48 154 33 49 16 686.14 2.48 155 33 50 17 685.99 2.47 156 34 50 16 686.15 2.46 157 34 51 17 685.99 2.46 158 36 39 3 801.43 2.35 159 36 40 4 789.90 1.83 160 36 47 11 686.39 2.48 161 36 48 12 686.39 2.48 162 36 49 13 686.23 2.44 163 37 40 3 801.41 2.35 164 37 41 4 790.53 2.15 165 37 47 10 686.43 2.47 166 37 49 12 686.38 2.48 167 38 41 3 802.70 1.72 168 38 42 4 790.55 2.15 169 39 42 3 803.58 1.27 170 39 43 4 789.94 1.84 171 40 43 3 800.06 3.02 172 40 44 4 789.78 1.89 173 40 45 5 687.70 1.95 174 40 46 6 687.70 1.95 175 40 47 7 686.53 2.42 176 41 44 3 796.29 4.51 177 46 67 21 622.33 0.41 178 46 68 22 622.33 0.40 179 48 60 12 622.84 0.44 180 48 63 15 622.59 0.34 181 48 64 16 622.57 0.34 182 48 67 19 622.58 0.34 183 49 63 14 623.06 0.39 184 50 55 5 623.64 0.38 185 50 60 10 623.07 0.39 186 50 63 13 623.08 0.38 187 51 54 3 804.01 0.06 188 51 55 4 623.70 0.40 189 52 60 8 623.29 0.30 190 52 76 24 622.31 0.35 191 52 78 26 622.12 0.34 192 52 99 47 435.16 3.30 193 52 100 48 435.14 3.29 194 52 104 52 431.22 2.43 195 52 108 56 430.18 2.87 196 53 80 27 622.12 0.34 197 53 96 43 435.32 3.29 198 53 99 46 435.16 3.30 199 53 100 47 435.14 3.29 200 56 59 3 804.25 0.00 201 56 60 4 797.96 1.67 202 57 60 3 797.97 1.66 203 57 61 4 797.96 1.67 204 57 104 47 430.35 2.85 205 57 107 50 429.92 2.98 206 57 108 51 429.94 2.98 207 58 61 3 818.91 0.56 208 58 62 4 784.83 4.90 209 58 107 49 429.92 2.98 210 58 110 52 429.89 2.98 211 58 111 53 429.73 2.98 212 59 62 3 818.91 0.56 213 59 63 4 787.81 2.51 214 60 63 3 795.99 0.94 215 60 64 4 787.81 2.51 216 60 108 48 429.91 2.98 217 61 64 3 798.43 7.81 218 61 65 4 786.80 2.00 219 61 71 10 622.84 0.57 220 61 108 47 429.88 2.99 221 61 111 50 420.72 0.04 222 61 112 51 415.99 2.08 223 61 115 54 415.72 1.95 224 62 65 3 799.56 7.25 225 62 66 4 786.80 2.00 226 63 66 3 804.39 1.51 227 63 67 4 790.34 5.23 228 64 67 3 796.17 2.31 229 64 68 4 779.06 1.00 230 65 68 3 804.66 0.40 231 65 69 4 765.30 0.64 232 65 70 5 623.31 0.47 233 65 71 6 622.81 0.59 234 65 115 50 415.50 2.02 235 68 71 3 862.56 2.36 236 71 112 41 415.81 2.08 237 71 115 44 415.50 2.02 238 71 116 45 414.81 2.35 239 72 116 44 415.34 2.10 240 72 119 47 413.19 1.54 241 72 121 49 412.95 1.56 242 72 122 50 412.82 1.52 243 72 123 51 412.81 1.52 244 73 116 43 415.53 2.02 245 73 121 48 413.04 1.58 246 73 123 50 412.53 1.65 247 73 218 145 90.16 3.73 248 74 108 34 429.79 3.00 249 74 109 35 429.09 3.19 250 74 112 38 415.94 2.06 251 74 116 42 415.60 2.00 252 74 123 49 412.93 1.57 253 74 124 50 412.51 1.65 254 74 125 51 412.51 1.64 255 75 125 50 412.48 1.65 256 75 127 52 411.74 1.32 257 75 218 143 90.17 3.71 258 76 100 24 435.07 3.30 259 76 101 25 432.18 3.72 260 76 104 28 430.33 2.85 261 76 105 29 429.88 2.99 262 76 108 32 429.85 2.98 263 76 125 49 412.51 1.65 264 76 127 51 411.74 1.32 265 77 127 50 411.74 1.32 266 78 100 22 435.11 3.29 267 79 100 21 435.14 3.31 268 79 101 22 435.12 3.30 269 79 181 102 186.62 1.43 270 80 96 16 435.47 3.23 271 80 97 17 435.30 3.31 272 80 100 20 435.18 3.31 273 80 101 21 435.15 3.31 274 81 97 16 435.46 3.24 275 81 141 60 337.70 0.30 276 81 144 63 337.46 0.38 277 82 89 7 436.04 3.25 278 82 93 11 435.57 3.25 279 82 96 14 435.54 3.25 280 82 97 15 435.51 3.25 281 83 139 56 338.94 0.01 282 83 140 57 338.81 0.01 283 83 141 58 338.50 0.08 284 83 181 98 202.75 1.92 285 83 184 101 188.23 1.63 286 84 88 4 731.77 17.86 287 84 89 5 442.61 0.07 288 84 93 9 436.14 3.29 289 84 140 56 338.92 0.00 290 84 142 58 338.87 0.01 291 85 88 3 786.85 7.28 292 85 89 4 643.83 61.83 293 85 140 55 338.94 0.01 294 85 142 57 338.89 0.01 295 86 89 3 785.97 0.30 296 86 142 56 338.89 0.01 297 89 93 4 793.05 4.13 298 89 94 5 793.01 4.12 299 89 142 53 338.87 0.01 300 89 143 54 338.71 0.07 301 90 93 3 794.26 3.52 302 90 94 4 796.13 5.67 303 91 94 3 802.02 2.73 304 91 95 4 796.66 5.41 305 92 95 3 797.52 4.99 306 92 96 4 796.68 5.42 307 93 96 3 796.69 5.42 308 93 97 4 796.18 5.67 309 94 97 3 829.00 3.06 310 94 98 4 797.01 5.25 311 94 143 49 337.52 0.39 312 94 146 52 337.62 0.35 313 95 98 3 797.01 5.25 314 95 99 4 796.16 5.67 315 95 103 8 571.01 27.54 316 96 99 3 816.41 2.06 317 96 100 4 801.03 2.01 318 97 100 3 820.43 1.64 319 97 101 4 791.51 4.62 320 97 143 46 337.45 0.38 321 97 144 47 337.47 0.38 322 97 147 50 335.01 0.98 323 98 101 3 809.30 3.93 324 98 102 4 770.59 4.30 325 98 103 5 581.65 31.69 326 98 143 45 337.44 0.38 327 98 146 48 337.17 0.27 328 98 147 49 332.66 2.11 329 99 103 4 580.48 32.27 330 99 104 5 572.63 36.20 331 100 104 4 512.03 8.21 332 101 105 4 784.35 5.62 333 101 144 43 337.44 0.39 334 101 147 46 332.54 2.14 335 101 148 47 332.01 1.89 336 101 151 50 312.47 2.71 337 102 105 3 791.78 3.69 338 102 106 4 793.90 2.65 339 102 150 48 324.95 0.68 340 102 151 49 312.65 2.62 341 102 154 52 306.64 0.17 342 103 106 3 798.29 0.47 343 103 107 4 798.31 0.48 344 104 107 3 800.96 0.06 345 104 108 4 793.95 2.66 346 105 108 3 809.89 3.79 347 105 109 4 793.97 2.65 348 105 126 21 412.77 1.66 349 105 151 46 307.01 0.01 350 105 154 49 294.92 4.01 351 106 109 3 808.36 4.54 352 106 110 4 793.96 2.65 353 106 154 48 294.89 4.00 354 107 110 3 794.28 2.49 355 107 111 4 790.53 0.93 356 108 111 3 803.74 1.55 357 108 112 4 796.68 2.15 358 109 112 3 808.71 1.06 359 109 113 4 805.95 2.48 360 109 124 15 413.01 1.58 361 109 126 17 412.95 1.57 362 109 158 49 276.62 0.03 363 109 162 53 276.55 0.01 364 110 114 4 826.09 4.34 365 110 162 52 276.55 0.01 366 111 114 3 852.20 2.50 367 111 115 4 826.10 4.34 368 112 115 3 826.10 4.34 369 112 116 4 826.07 4.35 370 113 116 3 814.10 1.64 371 113 117 4 810.43 3.47 372 113 162 49 276.48 0.01 373 113 167 54 276.31 0.02 374 114 117 3 804.25 4.40 375 114 118 4 790.23 1.10 376 115 118 3 792.60 1.43 377 115 119 4 787.23 1.26 378 116 119 3 792.60 1.43 379 116 120 4 784.23 0.21 380 116 121 5 509.60 1.62 381 116 122 6 413.06 1.59 382 116 123 7 423.57 3.66 383 116 124 8 413.06 1.59 384 116 167 51 276.10 0.05 385 117 120 3 787.20 1.28 386 117 165 48 276.31 0.02 387 117 167 50 276.23 0.00 388 120 165 45 276.27 0.02 389 120 166 46 276.18 0.01 390 121 166 45 276.23 0.01 391 121 218 97 90.50 3.88 392 121 219 98 90.47 3.89 393 121 220 99 90.03 3.76 394 122 166 44 276.31 0.02 395 122 167 45 276.01 0.05 396 122 168 46 276.01 0.05 397 123 168 45 276.05 0.06 398 123 217 94 90.66 3.79 399 123 218 95 90.25 3.77 400 123 219 96 90.15 3.72 401 123 220 97 90.13 3.72 402 123 222 99 69.95 0.46 403 123 223 100 69.27 0.12 404 124 162 38 276.51 0.00 405 124 167 43 276.34 0.03 406 124 168 44 276.22 0.00 407 124 169 45 275.88 0.07 408 124 170 46 275.53 0.10 409 125 170 45 273.85 0.81 410 125 172 47 272.96 0.51 411 125 214 89 90.93 3.85 412 125 217 92 90.67 3.79 413 125 218 93 90.33 3.63 414 126 151 25 306.04 0.42 415 126 154 28 291.00 5.28 416 126 155 29 278.53 0.82 417 126 158 32 277.08 0.13 418 126 169 43 276.34 0.04 419 126 170 44 276.05 0.07 420 126 171 45 273.75 0.80 421 126 172 46 273.75 0.81 422 127 148 21 331.90 1.89 423 127 151 24 307.00 0.00 424 127 171 44 273.80 0.80 425 127 172 45 273.54 0.78 426 127 174 47 272.52 0.48 427 128 132 4 774.28 0.07 428 128 148 20 331.88 1.89 429 128 171 43 274.42 0.48 430 128 172 44 273.81 0.79 431 128 173 45 272.95 0.62 432 128 174 46 272.95 0.62 433 129 132 3 791.34 6.09 434 129 144 15 337.46 0.38 435 129 148 19 332.11 1.94 436 129 173 44 273.61 0.81 437 129 174 45 273.51 0.79 438 130 133 3 805.67 0.58 439 130 134 4 774.26 0.07 440 130 174 44 273.69 0.78 441 130 175 45 273.31 0.77 442 130 176 46 273.42 0.83 443 130 233 103 55.77 2.21 444 130 236 106 41.31 0.29 445 130 237 107 35.62 0.01 446 131 134 3 764.93 4.60 447 131 135 4 764.91 4.61 448 131 136 5 339.06 0.03 449 131 141 10 338.44 0.04 450 131 144 13 337.47 0.38 451 131 145 14 336.92 0.32 452 131 148 17 332.70 2.11 453 131 149 18 332.62 2.12 454 132 148 16 332.62 2.12 455 132 149 17 332.58 2.13 456 132 152 20 332.06 1.90 457 132 171 39 276.38 0.04 458 132 173 41 275.67 0.12 459 133 152 19 332.56 2.14 460 133 173 40 276.34 0.04 461 133 229 96 66.09 0.62 462 133 237 104 38.86 1.27 463 134 145 11 337.57 0.35 464 134 237 103 44.08 1.10 465 135 145 10 337.55 0.34 466 136 139 3 799.05 5.06 467 136 141 5 338.57 0.01 468 138 191 53 165.36 2.87 469 138 195 57 162.27 1.32 470 139 185 46 188.26 1.61 471 139 191 52 165.38 2.86 472 139 195 56 162.30 1.32 473 141 144 3 794.82 5.26 474 141 145 4 786.84 4.69 475 142 145 3 801.95 2.85 476 142 146 4 800.22 2.00 477 143 146 3 802.68 3.23 478 143 147 4 791.48 2.35 479 144 147 3 800.18 2.02 480 144 148 4 795.54 0.30 481 145 148 3 809.52 0.24 482 145 149 4 789.51 2.72 483 146 149 3 809.53 0.24 484 146 150 4 791.60 1.68 485 147 150 3 801.88 3.64 486 147 151 4 798.58 1.97 487 148 151 3 801.91 0.29 488 149 152 3 812.59 2.02 489 150 153 3 805.30 1.23 490 150 154 4 796.40 4.78 491 151 154 3 800.80 2.59 492 151 155 4 790.21 1.68 493 151 171 20 276.33 0.04 494 152 155 3 802.37 3.38 495 152 156 4 794.61 0.51 496 152 171 19 276.39 0.04 497 152 229 77 59.00 1.09 498 153 156 3 800.03 0.79 499 153 157 4 790.18 1.73 500 154 157 3 799.18 4.21 501 154 158 4 788.50 2.64 502 155 158 3 816.88 0.12 503 155 159 4 788.52 2.63 504 155 169 14 276.41 0.04 505 155 170 15 276.39 0.04 506 155 171 16 276.36 0.03 507 155 227 72 59.09 1.05 508 155 228 73 59.02 1.09 509 156 159 3 795.15 0.01 510 156 160 4 788.53 2.63 511 156 227 71 59.09 1.05 512 157 160 3 808.80 3.81 513 157 161 4 789.20 2.96 514 158 161 3 801.46 3.12 515 158 162 4 789.20 2.96 516 158 169 11 276.40 0.04 517 159 162 3 811.55 4.71 518 159 163 4 783.31 5.91 519 159 169 10 276.40 0.04 520 159 226 67 59.80 0.69 521 159 227 68 58.86 1.01 522 162 165 3 798.55 4.70 523 162 166 4 786.52 1.31 524 162 167 5 276.56 0.01 525 162 169 7 276.44 0.01 526 163 166 3 793.52 1.96 527 163 167 4 648.32 64.78 528 163 168 5 429.88 42.04 529 168 222 54 78.71 0.12 530 168 223 55 69.56 0.27 531 168 225 57 59.41 0.88 532 168 226 58 58.76 1.04 533 169 226 57 59.00 1.08 534 169 227 58 58.96 1.07 535 170 217 47 90.70 3.77 536 170 225 55 67.94 0.18 537 170 226 56 59.02 1.09 538 170 227 57 58.96 1.07 539 170 228 58 57.59 1.60 540 171 228 57 58.75 1.16 541 171 229 58 56.12 2.10 542 171 230 59 56.08 2.12 543 172 210 38 94.82 2.57 544 172 213 41 93.97 2.38 545 172 214 42 93.75 2.44 546 172 217 45 91.36 3.46 547 172 230 58 55.75 2.21 548 172 232 60 55.52 2.22 549 173 229 56 56.50 2.11 550 173 230 57 56.18 2.13 551 173 231 58 55.61 2.24 552 173 232 59 55.56 2.25 553 174 177 3 873.39 0.80 554 174 210 36 94.83 2.58 555 174 232 58 55.47 2.25 556 175 232 57 55.58 2.25 557 175 233 58 53.44 1.75 558 176 185 9 189.94 2.89 559 176 195 19 103.48 1.25 560 176 199 23 103.21 1.11 561 176 204 28 103.06 1.03 562 176 205 29 103.06 1.03 563 176 236 60 45.12 0.59 564 177 182 5 781.33 1.04 565 177 204 27 103.05 1.03 566 177 205 28 102.80 1.15 567 177 206 29 102.78 1.14 568 177 207 30 102.75 1.14 569 177 210 33 102.59 1.17 570 178 182 4 801.74 1.66 571 178 186 8 344.36 79.80 572 178 204 26 103.03 1.05 573 178 205 27 102.81 1.16 574 178 206 28 102.72 1.18 575 178 207 29 102.59 1.21 576 179 182 3 810.88 1.46 577 179 183 4 803.05 2.31 578 179 186 7 575.17 29.27 579 179 207 28 102.56 1.20 580 180 183 3 811.49 1.77 581 180 184 4 826.57 0.74 582 181 184 3 877.02 7.10 583 182 185 3 822.02 3.02 584 182 186 4 811.84 2.08 585 182 204 22 103.09 1.05 586 183 186 3 803.99 2.17 587 183 187 4 794.71 1.33 588 184 187 3 803.66 2.01 589 185 191 6 167.53 1.50 590 185 192 7 162.23 1.30 591 185 195 10 162.24 1.30 592 185 204 19 105.08 0.51 593 186 192 6 162.01 1.25 594 186 204 18 103.24 1.11 595 188 191 3 815.84 0.46 596 188 192 4 783.22 5.06 597 189 192 3 798.20 0.61 598 189 193 4 702.03 35.54 599 189 194 5 781.02 0.60 600 190 194 4 803.90 1.98 601 191 194 3 826.23 3.81 602 191 195 4 802.41 2.73 603 192 195 3 803.23 2.82 604 192 196 4 792.50 1.02 605 192 204 12 110.86 1.12 606 193 196 3 809.66 0.40 607 193 197 4 784.04 5.32 608 194 197 3 810.73 1.41 609 194 198 4 802.97 2.47 610 195 198 3 802.98 2.48 611 195 199 4 802.97 2.47 612 195 204 9 117.84 0.72 613 195 236 41 55.47 2.29 614 196 199 3 802.96 2.48 615 196 202 6 133.84 6.53 616 196 204 8 117.65 0.60 617 197 239 42 53.78 1.72 618 198 236 38 55.48 2.27 619 198 239 41 41.06 0.41 620 199 202 3 785.70 2.19 621 199 203 4 714.55 28.96 622 199 204 5 437.99 46.38 623 199 233 34 55.66 2.29 624 199 234 35 55.55 2.25 625 199 236 37 55.29 2.33 626 200 234 34 55.54 2.23 627 200 235 35 55.50 2.23 628 200 236 36 55.45 2.25 629 200 239 39 35.72 0.01 630 201 234 33 55.50 2.23 631 203 209 6 799.10 4.11 632 205 209 4 793.71 6.80 633 205 210 5 793.70 6.80 634 206 209 3 802.70 2.31 635 206 210 4 802.70 2.31 636 207 210 3 804.49 1.42 637 207 211 4 793.71 6.80 638 208 211 3 806.16 7.66 639 208 212 4 799.06 4.11 640 209 212 3 815.84 4.29 641 210 213 3 819.37 2.52 642 210 214 4 800.95 3.27 643 210 232 22 54.41 1.95 644 211 214 3 800.95 3.27 645 211 215 4 800.95 3.27 646 212 215 3 806.38 0.55 647 212 216 4 800.95 3.27 648 213 216 3 800.96 3.25 649 213 217 4 800.96 3.25 650 213 230 17 55.67 2.19 651 213 232 19 53.72 1.47 652 214 217 3 801.30 3.41 653 214 218 4 796.22 0.87 654 215 218 3 802.71 0.39 655 215 219 4 786.42 4.03 656 216 219 3 801.10 9.38 657 216 221 5 303.10 78.58 658 216 222 6 141.94 2.00 659 217 222 5 141.94 2.00 660 217 225 8 123.55 1.02 661 217 230 13 55.65 2.18 662 219 222 3 820.04 1.72 663 220 223 3 798.77 12.35 664 222 225 3 693.56 17.93 665 225 228 3 868.23 6.49 666 231 237 6 139.42 1.28 667 233 236 3 809.76 5.53 668 233 237 4 786.40 2.10 669 235 238 3 786.83 1.74 670 235 239 4 713.35 34.42 671 236 239 3 808.86 12.75