BSDB

The conformational change and the identification of the mechanical clamp for proteins can be derived by looking at the unfolding scenario. A unfolding scenario shows distance d_u at which a given native contact is broken for the last time. Contacts in the scenario diagrams are identified by the sequential distance |j - i|. A contact is said to be broken if the distance between amino acids i and j exceeds 1.5σ_ij. An accumulation of many contacts unfolding events at a value of d_u indicates emergence of a force peak. In such figure the y-axis shows the sequential separation between two amino acids that make a native contact. The x-axis indicates at which displacement of the pulling tip the contact is broken for the last time. Notice that thermal fluctuations may reinstate a broken contact temporarily and that’s why we seek the last, and thus definitive, rupture event.

As an example we show the unfolding diagram for the first chain of protein 1c4p. This protein contains an α helix (196-210), denoted as I, and eight β strands, denoted as A through H, as labelled consecutively from the N to C termini. These β strands form three β sheets: a, b, and c. The a sheet comprises four strands (A, B, G, E), b sheet two ( C, F), and c-sheet also two ( D, H). These letters are indicated on the unfolding scenario figure and show which secondary structures are involved in a contact that is broken at the distance d_u. The data symbols marked by asterisks correspond to contacts which do not involve any secondary structures. The remaining symbols are diversified and have a meaning identified by the letter symbols placed next to them. The force peak at about 140 Å involves a near simultaneous rupture of the contacts which relate to the terminal strands A and G: A+G (meaning between A and G), A+I, G+I (i.e. with the helix) as well as D+H, A+B, E+G, D+G, C+G, and A+C. Breaking these contacts results in destroying the a β-sheet. Later on, the remaining contacts, such as C+F in the b sheet are broken and the helix is the last to unravel.

ICN I J |I-J| Displacement error 1 3 220 217 25.63 0.42 2 3 224 221 25.79 0.49 3 3 226 223 25.80 0.49 4 4 8 4 805.52 0.57 5 5 8 3 799.82 3.42 6 5 27 22 727.16 2.85 7 5 30 25 741.21 1.91 8 5 32 27 738.82 0.83 9 5 79 74 548.53 0.27 10 5 220 215 31.99 0.53 11 5 223 218 31.97 0.53 12 5 224 219 31.98 0.54 13 6 30 24 745.77 2.79 14 6 31 25 741.89 1.55 15 6 32 26 741.89 1.55 16 7 31 24 747.20 3.49 17 7 32 25 747.18 3.48 18 7 33 26 747.17 3.48 19 8 32 24 747.21 3.48 20 8 33 25 747.21 3.48 21 8 78 70 580.05 0.32 22 8 79 71 580.03 0.33 23 8 129 121 346.79 26.60 24 8 225 217 95.38 0.43 25 9 33 24 747.22 3.48 26 9 35 26 747.21 3.48 27 9 55 46 673.65 1.40 28 9 74 65 580.25 0.34 29 9 75 66 580.23 0.34 30 9 76 67 580.19 0.34 31 9 77 68 580.09 0.32 32 9 78 69 580.06 0.33 33 9 79 70 580.06 0.33 34 10 27 17 747.46 3.44 35 10 32 22 747.43 3.45 36 10 33 23 747.36 3.45 37 10 34 24 747.21 3.48 38 10 35 25 747.21 3.48 39 10 77 67 580.15 0.34 40 10 79 69 580.06 0.33 41 10 81 71 580.03 0.33 42 10 223 213 98.95 1.26 43 11 35 24 747.22 3.48 44 11 37 26 747.20 3.48 45 11 71 60 580.43 0.31 46 11 74 63 580.29 0.32 47 11 77 66 580.20 0.34 48 11 79 68 580.10 0.33 49 11 80 69 580.07 0.33 50 11 81 70 580.05 0.33 51 11 114 103 351.07 25.37 52 12 15 3 790.02 4.76 53 12 20 8 747.99 3.39 54 12 23 11 747.60 3.44 55 12 24 12 747.53 3.46 56 12 27 15 747.52 3.45 57 12 34 22 747.43 3.45 58 12 35 23 747.35 3.45 59 12 36 24 747.23 3.48 60 12 37 25 747.21 3.48 61 12 81 69 580.03 0.32 62 13 37 24 747.22 3.48 63 13 61 48 586.25 0.88 64 13 80 67 580.18 0.34 65 13 81 68 580.07 0.33 66 13 82 69 580.03 0.33 67 13 83 70 579.98 0.32 68 13 84 71 578.00 0.17 69 13 114 101 351.41 25.23 70 14 17 3 829.25 2.98 71 14 20 6 748.38 3.22 72 14 37 23 747.34 3.45 73 14 83 69 579.72 0.30 74 14 84 70 578.03 0.18 75 15 20 5 748.39 3.22 76 15 21 6 748.39 3.22 77 15 24 9 748.36 3.21 78 15 36 21 747.37 3.45 79 15 37 22 747.35 3.45 80 15 38 23 747.29 3.44 81 15 47 32 674.75 1.27 82 16 21 5 750.90 3.97 83 16 38 22 747.34 3.45 84 16 43 27 675.46 1.53 85 17 20 3 789.47 3.40 86 17 21 4 750.94 3.95 87 17 43 26 675.47 1.52 88 17 185 168 192.58 0.93 89 18 21 3 794.47 0.92 90 18 22 4 766.33 8.17 91 18 184 166 192.63 0.96 92 18 212 194 114.89 1.77 93 19 22 3 783.05 1.03 94 19 23 4 775.25 3.71 95 19 83 64 579.70 0.29 96 19 134 115 346.74 26.71 97 19 182 163 194.84 0.07 98 19 184 165 192.88 0.93 99 19 212 193 114.88 1.77 100 19 215 196 114.72 1.80 101 19 216 197 114.73 1.81 102 19 219 200 114.77 1.79 103 20 23 3 780.37 2.70 104 20 24 4 775.25 3.71 105 20 83 63 579.58 0.34 106 21 24 3 782.86 1.46 107 21 25 4 776.81 4.48 108 21 43 22 675.46 1.53 109 21 46 25 674.84 1.22 110 21 47 26 674.71 1.28 111 21 50 29 674.53 1.27 112 22 25 3 784.16 0.81 113 22 26 4 776.81 4.48 114 22 212 190 114.85 1.76 115 22 216 194 114.70 1.79 116 23 26 3 774.08 3.11 117 23 27 4 774.50 2.90 118 23 81 58 579.98 0.35 119 23 216 193 114.46 1.76 120 23 219 196 114.43 1.75 121 23 220 197 113.98 1.85 122 24 27 3 785.20 1.33 123 24 28 4 774.49 2.91 124 24 34 10 747.50 3.43 125 24 36 12 747.33 3.45 126 24 47 23 674.53 1.33 127 24 51 27 674.24 1.31 128 24 54 30 673.78 1.39 129 25 28 3 785.22 1.32 130 25 29 4 772.73 2.47 131 25 47 22 674.49 1.31 132 25 50 25 674.32 1.31 133 25 51 26 674.25 1.32 134 26 29 3 788.37 1.44 135 26 30 4 776.07 0.81 136 26 216 190 114.46 1.76 137 26 220 194 113.80 1.72 138 27 30 3 778.82 0.58 139 27 31 4 777.12 1.33 140 27 32 5 749.91 2.64 141 27 34 7 747.54 3.41 142 27 220 193 113.61 1.68 143 27 223 196 108.21 1.12 144 28 31 3 785.78 1.45 145 28 32 4 753.30 2.34 146 28 34 6 747.57 3.42 147 28 51 23 674.19 1.31 148 28 52 24 674.20 1.32 149 28 54 26 673.71 1.41 150 34 54 20 673.68 1.39 151 34 55 21 673.66 1.40 152 35 55 20 673.66 1.40 153 35 57 22 673.58 1.43 154 35 74 39 580.25 0.33 155 36 44 8 674.74 1.26 156 36 47 11 674.59 1.31 157 36 54 18 673.82 1.41 158 36 55 19 673.72 1.41 159 36 56 20 673.66 1.39 160 36 57 21 673.59 1.43 161 37 44 7 675.35 1.55 162 37 57 20 673.59 1.43 163 37 59 22 673.50 1.39 164 37 61 24 630.33 1.85 165 37 71 34 580.45 0.31 166 38 44 6 675.40 1.54 167 38 57 19 673.71 1.41 168 38 58 20 673.59 1.43 169 38 59 21 673.57 1.42 170 39 58 19 673.69 1.40 171 39 59 20 673.58 1.43 172 39 60 21 673.46 1.38 173 39 102 63 357.06 23.73 174 40 43 3 779.05 1.74 175 40 44 4 777.62 1.99 176 41 44 3 794.15 7.77 177 41 45 4 780.46 2.92 178 41 58 17 673.78 1.40 179 42 45 3 780.94 2.68 180 42 46 4 780.46 2.92 181 43 46 3 809.38 1.96 182 43 47 4 779.96 3.16 183 44 47 3 794.01 0.46 184 44 48 4 781.71 0.39 185 44 56 12 673.82 1.40 186 44 57 13 673.82 1.40 187 44 58 14 673.75 1.39 188 45 48 3 797.85 2.67 189 45 49 4 781.72 0.39 190 46 49 3 775.91 2.79 191 46 50 4 775.91 2.79 192 47 50 3 775.91 2.79 193 47 51 4 765.16 2.58 194 47 54 7 674.01 1.35 195 48 51 3 769.65 0.35 196 48 52 4 763.48 3.43 197 48 54 6 674.01 1.35 198 48 56 8 673.83 1.40 199 49 52 3 786.49 1.20 200 51 54 3 808.36 0.78 201 57 70 13 580.49 0.33 202 57 71 14 580.47 0.34 203 59 66 7 580.99 0.28 204 59 67 8 580.86 0.35 205 59 70 11 580.69 0.40 206 59 71 12 580.69 0.40 207 60 63 3 791.55 0.67 208 60 67 7 581.13 0.24 209 60 102 42 357.00 23.76 210 61 67 6 581.12 0.23 211 61 71 10 580.97 0.27 212 61 84 23 580.08 0.33 213 61 110 49 355.18 24.12 214 61 114 53 354.80 24.29 215 62 102 40 356.41 24.00 216 62 105 43 355.73 24.18 217 62 106 44 355.72 24.20 218 62 109 47 355.71 24.20 219 63 66 3 785.21 2.44 220 63 67 4 755.29 6.48 221 64 67 3 786.34 1.98 222 64 68 4 768.94 0.35 223 64 109 45 355.73 24.16 224 64 113 49 355.38 24.04 225 64 116 52 355.61 24.15 226 65 68 3 778.74 0.80 227 65 69 4 776.89 0.12 228 66 69 3 808.09 0.93 229 66 70 4 765.68 1.96 230 67 70 3 784.00 3.67 231 67 71 4 770.33 4.30 232 67 113 46 355.07 24.14 233 67 114 47 354.80 24.29 234 68 72 4 589.64 3.75 235 68 113 45 355.06 24.13 236 69 72 3 694.27 42.33 237 71 74 3 783.49 2.27 238 71 114 43 351.85 25.15 239 71 117 46 351.08 24.96 240 72 117 45 351.08 24.96 241 74 77 3 885.22 5.13 242 74 117 43 350.97 25.03 243 76 125 49 349.42 25.57 244 77 118 41 349.63 25.56 245 77 122 45 349.42 25.58 246 77 125 48 349.57 25.51 247 78 122 44 349.41 25.61 248 78 125 47 349.45 25.58 249 78 129 51 346.91 26.63 250 78 130 52 346.91 26.63 251 79 130 51 346.87 26.61 252 79 223 144 98.95 1.26 253 80 114 34 351.10 25.36 254 80 115 35 350.72 25.46 255 80 118 38 350.43 25.32 256 80 130 50 347.03 26.62 257 80 131 51 346.80 26.57 258 80 132 52 346.67 26.64 259 80 223 143 108.52 0.96 260 81 132 51 346.67 26.64 261 81 134 53 346.63 26.66 262 81 219 138 114.16 1.88 263 81 223 142 110.81 2.02 264 82 111 29 350.63 25.62 265 82 114 32 351.55 25.16 266 82 115 33 351.12 25.36 267 82 132 50 346.98 26.64 268 82 133 51 346.79 26.57 269 82 134 52 346.64 26.65 270 83 134 51 346.62 26.66 271 85 106 21 355.56 24.20 272 85 107 22 354.62 24.62 273 85 153 68 330.01 11.62 274 85 186 101 193.21 0.77 275 86 102 16 356.38 24.00 276 86 103 17 356.27 24.00 277 86 106 20 355.72 24.20 278 86 107 21 355.68 24.19 279 86 149 63 346.27 19.00 280 86 186 100 193.22 0.77 281 87 149 62 349.33 20.53 282 87 186 99 193.22 0.78 283 88 99 11 357.00 23.68 284 88 102 14 357.00 23.68 285 88 103 15 356.93 23.71 286 89 145 56 355.06 22.66 287 89 146 57 355.04 22.66 288 89 149 60 354.82 22.56 289 89 190 101 193.03 0.86 290 90 94 4 675.99 38.98 291 90 95 5 499.02 42.06 292 90 96 6 500.16 41.50 293 90 99 9 358.66 24.30 294 90 145 55 355.10 22.66 295 90 147 57 355.22 22.61 296 91 94 3 777.36 3.39 297 91 95 4 676.00 38.98 298 91 145 54 355.11 22.66 299 92 95 3 787.14 1.51 300 92 147 55 355.18 22.63 301 95 147 52 367.87 16.28 302 95 148 53 367.85 16.27 303 96 99 3 775.19 1.98 304 96 100 4 775.36 1.89 305 97 100 3 780.53 0.70 306 97 101 4 767.72 1.93 307 98 101 3 781.94 0.01 308 98 102 4 780.52 0.70 309 99 102 3 788.68 3.37 310 99 103 4 770.60 0.45 311 100 103 3 793.84 5.95 312 100 104 4 777.55 2.91 313 100 148 48 401.98 1.39 314 101 104 3 800.79 4.79 315 101 105 4 777.55 2.90 316 102 105 3 800.80 4.79 317 102 106 4 769.67 1.04 318 103 107 4 681.12 35.24 319 103 148 45 406.09 3.71 320 103 152 49 393.69 9.86 321 104 108 4 458.22 0.99 322 104 109 5 452.19 4.02 323 104 148 44 406.18 3.71 324 104 151 47 401.05 6.22 325 104 152 48 388.12 12.65 326 104 155 51 383.45 14.98 327 105 109 4 523.75 34.12 328 105 110 5 664.30 41.20 329 106 110 4 768.86 1.28 330 107 111 4 780.97 0.25 331 107 149 42 399.63 7.12 332 107 152 45 383.64 15.06 333 107 156 49 380.79 16.48 334 108 111 3 783.38 1.46 335 108 112 4 780.97 0.25 336 108 155 47 381.69 16.05 337 108 156 48 380.80 16.48 338 108 159 51 380.63 16.55 339 109 112 3 781.70 0.62 340 109 113 4 771.71 0.22 341 110 113 3 771.71 0.23 342 110 114 4 771.67 0.22 343 111 115 4 730.38 21.95 344 111 133 22 407.10 3.38 345 111 156 45 380.72 16.56 346 111 159 48 374.33 19.74 347 112 115 3 775.13 0.42 348 112 116 4 770.43 1.93 349 112 159 47 374.33 19.74 350 113 116 3 795.36 5.75 351 113 117 4 775.19 0.39 352 114 117 3 779.91 1.97 353 114 118 4 772.48 0.96 354 115 119 4 775.31 0.53 355 115 131 16 407.17 3.40 356 115 163 48 357.44 24.45 357 116 119 3 786.00 4.21 358 116 120 4 775.38 0.49 359 117 120 3 792.74 7.57 360 117 121 4 774.57 2.40 361 118 121 3 778.47 0.45 362 118 122 4 778.09 0.84 363 119 122 3 782.55 1.38 364 119 123 4 778.06 0.86 365 119 167 48 350.57 21.25 366 119 172 53 339.29 15.84 367 120 123 3 778.05 0.86 368 120 124 4 778.05 0.86 369 121 124 3 796.87 0.96 370 121 125 4 767.86 1.68 371 122 125 3 775.33 0.45 372 122 126 4 765.43 2.88 373 122 127 5 487.79 3.41 374 122 128 6 472.25 8.62 375 122 129 7 411.62 1.98 376 122 172 50 340.42 16.34 377 123 126 3 767.02 3.70 378 123 167 44 354.54 23.19 379 123 170 47 348.01 20.05 380 123 172 49 340.62 16.40 381 128 171 43 340.43 16.29 382 129 171 42 340.47 16.30 383 129 172 43 339.45 15.82 384 129 173 44 339.45 15.82 385 130 172 42 339.38 15.83 386 130 173 43 327.24 9.77 387 130 223 93 109.48 1.36 388 131 163 32 361.31 26.12 389 131 167 36 351.55 21.37 390 131 172 41 339.40 15.84 391 131 173 42 327.08 9.85 392 131 174 43 311.54 3.35 393 131 175 44 304.27 0.29 394 132 175 43 303.79 0.45 395 132 177 45 303.70 0.47 396 132 219 87 114.17 1.88 397 132 222 90 112.67 1.32 398 132 223 91 110.96 1.94 399 133 156 23 374.30 20.05 400 133 160 27 361.79 26.11 401 133 174 41 304.12 0.32 402 133 175 42 303.73 0.48 403 133 176 43 303.51 0.49 404 133 177 44 303.51 0.49 405 134 153 19 382.85 16.46 406 134 156 22 381.12 16.84 407 134 177 43 303.15 0.33 408 134 219 85 114.75 1.79 409 135 139 4 986.57 0.69 410 135 153 18 382.49 16.29 411 135 156 21 381.24 16.87 412 135 157 22 381.19 16.90 413 135 176 41 303.17 0.33 414 135 177 42 303.15 0.33 415 135 178 43 302.98 0.32 416 135 179 44 302.98 0.32 417 136 139 3 987.94 0.03 418 136 149 13 862.09 0.69 419 136 153 17 384.56 15.62 420 136 177 41 303.06 0.31 421 136 178 42 301.25 1.15 422 136 179 43 301.25 1.15 423 136 242 106 65.88 0.74 424 137 178 41 301.28 1.16 425 137 179 42 301.25 1.15 426 137 180 43 301.07 1.18 427 137 200 63 131.19 1.53 428 137 238 101 90.10 0.67 429 137 241 104 76.71 0.07 430 137 242 105 65.00 0.32 431 139 150 11 987.88 0.06 432 139 153 14 546.64 24.41 433 139 154 15 492.56 2.64 434 139 157 18 492.53 2.62 435 139 178 39 301.87 0.88 436 140 157 17 497.97 0.10 437 140 234 94 94.64 0.24 438 140 242 102 67.50 0.55 439 141 241 100 89.92 0.58 440 141 242 101 77.89 0.23 441 143 191 48 196.27 0.67 442 149 153 4 963.22 2.99 443 150 153 3 763.49 0.39 444 150 154 4 769.94 2.43 445 151 154 3 776.99 3.06 446 151 155 4 777.03 3.06 447 152 155 3 787.12 1.71 448 152 156 4 778.88 2.15 449 153 156 3 789.94 3.36 450 153 157 4 796.16 0.24 451 154 157 3 799.11 1.13 452 154 158 4 798.61 0.88 453 155 158 3 808.42 0.57 454 155 159 4 846.69 4.03 455 156 159 3 987.24 0.10 456 156 160 4 863.69 1.24 457 157 160 3 987.99 0.01 458 157 161 4 919.72 3.83 459 157 178 21 303.04 0.34 460 157 234 77 91.97 0.83 461 158 162 4 869.82 1.94 462 159 162 3 987.75 0.05 463 159 163 4 841.88 2.71 464 160 163 3 970.48 8.54 465 160 164 4 841.75 2.65 466 160 174 14 303.75 0.43 467 160 175 15 303.74 0.43 468 160 176 16 303.66 0.45 469 160 232 72 92.00 0.83 470 161 164 3 800.41 1.62 471 161 165 4 786.85 1.18 472 161 232 71 92.00 0.83 473 161 234 73 92.00 0.83 474 162 165 3 786.85 1.18 475 162 166 4 789.05 0.17 476 163 166 3 789.47 0.05 477 163 167 4 789.48 0.06 478 163 174 11 309.58 1.36 479 164 167 3 794.00 2.31 480 164 168 4 783.14 3.11 481 164 174 10 309.58 1.36 482 164 231 67 92.00 0.83 483 164 232 68 91.81 0.75 484 165 168 3 801.02 0.43 485 165 169 4 801.68 0.67 486 166 169 3 852.60 1.27 487 167 170 3 800.81 3.70 488 167 171 4 782.40 3.58 489 167 172 5 473.27 0.15 490 167 174 7 361.83 27.12 491 168 171 3 790.75 0.59 492 168 172 4 775.61 6.53 493 173 222 49 112.75 1.35 494 173 223 50 111.27 2.08 495 173 224 51 111.19 2.04 496 173 225 52 110.92 2.02 497 173 227 54 98.78 1.32 498 173 228 55 95.35 0.44 499 173 230 57 91.99 0.83 500 173 231 58 91.63 0.70 501 174 231 57 91.97 0.83 502 174 232 58 91.72 0.76 503 175 222 47 113.87 1.91 504 175 230 55 94.98 0.45 505 175 231 56 91.98 0.83 506 175 232 57 91.72 0.75 507 175 233 58 91.11 0.65 508 176 233 57 91.59 0.70 509 176 234 58 90.45 0.83 510 176 235 59 90.20 0.71 511 177 215 38 114.72 1.81 512 177 218 41 114.70 1.81 513 177 219 42 114.77 1.79 514 177 222 45 114.59 1.76 515 177 235 58 90.19 0.70 516 177 237 60 89.98 0.62 517 178 234 56 90.58 0.80 518 178 235 57 90.48 0.83 519 178 236 58 90.15 0.69 520 178 237 59 90.09 0.68 521 178 238 60 89.97 0.62 522 178 242 64 64.49 0.57 523 179 182 3 875.58 0.96 524 179 215 36 115.16 1.81 525 179 237 58 89.95 0.65 526 179 238 59 89.81 0.60 527 180 204 24 116.59 2.01 528 180 209 29 116.16 2.00 529 180 210 30 116.10 1.99 530 180 237 57 90.17 0.70 531 180 238 58 89.69 0.55 532 181 187 6 193.27 0.80 533 181 191 10 190.12 0.33 534 181 200 19 134.76 6.28 535 181 209 28 116.11 2.03 536 181 210 29 116.09 2.02 537 182 187 5 196.16 0.65 538 182 210 28 115.89 2.07 539 182 211 29 115.88 2.06 540 182 212 30 115.87 2.06 541 182 215 33 115.87 2.06 542 183 187 4 319.81 61.09 543 183 188 5 196.24 0.69 544 183 191 8 193.19 0.82 545 183 192 9 191.34 0.29 546 183 209 26 116.09 2.02 547 184 187 3 562.50 16.39 548 184 188 4 460.09 0.37 549 184 212 28 115.70 1.99 550 185 189 4 762.13 0.74 551 186 189 3 792.28 1.11 552 187 191 4 622.63 69.00 553 189 192 3 788.78 1.08 554 190 196 6 433.75 0.91 555 191 196 5 433.75 0.91 556 191 197 6 312.12 55.00 557 191 200 9 311.31 54.59 558 193 196 3 780.07 4.08 559 193 197 4 772.08 0.09 560 194 197 3 779.13 3.44 561 194 198 4 766.73 3.64 562 195 198 3 789.81 7.23 563 195 199 4 768.99 2.51 564 196 199 3 791.75 1.67 565 197 200 3 781.22 3.60 566 197 201 4 777.06 5.65 567 197 209 12 131.32 0.42 568 198 201 3 780.25 4.05 569 198 202 4 780.24 4.05 570 199 202 3 797.57 0.19 571 199 203 4 783.76 5.79 572 200 203 3 795.74 1.10 573 200 204 4 774.17 1.00 574 200 209 9 158.63 1.96 575 200 241 41 90.10 0.72 576 201 204 3 795.11 1.42 577 201 207 6 162.50 3.89 578 201 208 7 162.50 3.88 579 201 209 8 158.62 1.95 580 203 241 38 90.14 0.71 581 203 244 41 82.33 0.57 582 204 207 3 677.94 41.31 583 204 208 4 621.74 69.41 584 204 209 5 606.46 71.95 585 204 238 34 90.28 0.75 586 204 239 35 90.19 0.71 587 204 241 37 89.91 0.60 588 205 239 34 90.21 0.72 589 205 240 35 90.14 0.70 590 205 241 36 90.14 0.70 591 205 244 39 70.26 0.46 592 206 239 33 90.04 0.65 593 210 214 4 777.33 8.28 594 210 237 27 89.96 0.62 595 211 214 3 789.71 2.10 596 211 215 4 777.34 8.28 597 212 215 3 796.69 2.67 598 212 216 4 770.58 11.66 599 213 216 3 777.34 8.28 600 213 217 4 713.15 40.37 601 214 217 3 778.73 3.37 602 214 218 4 652.14 17.71 603 214 237 23 89.97 0.63 604 215 218 3 694.37 38.82 605 215 219 4 652.14 17.71 606 215 237 22 89.85 0.68 607 216 219 3 759.23 2.25 608 216 220 4 683.92 33.59 609 217 220 3 747.09 20.73 610 217 221 4 687.63 35.42 611 218 221 3 778.42 4.19 612 218 222 4 692.25 37.48 613 218 235 17 90.14 0.69 614 218 237 19 89.86 0.68 615 219 222 3 767.76 0.28 616 219 223 4 683.91 33.59 617 220 223 3 759.00 0.27 618 220 224 4 602.65 2.79 619 221 224 3 752.63 3.45 620 221 226 5 470.03 15.79 621 221 227 6 440.84 4.80 622 222 227 5 433.86 8.29 623 222 230 8 149.75 2.44 624 222 233 11 106.49 8.34 625 222 235 13 90.17 0.70 626 224 227 3 786.19 2.65 627 225 228 3 591.35 0.10 628 227 230 3 758.65 5.44 629 230 233 3 811.14 3.70 630 234 242 8 164.54 1.35 631 236 242 6 373.36 28.82 632 238 241 3 777.45 1.86 633 238 242 4 759.47 4.91 634 240 243 3 775.64 1.20 635 240 244 4 774.55 1.74 636 241 244 3 800.66 2.79